CN102668854A - Cutting propagation method for momordica grosvenori - Google Patents
Cutting propagation method for momordica grosvenori Download PDFInfo
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- CN102668854A CN102668854A CN2012101733392A CN201210173339A CN102668854A CN 102668854 A CN102668854 A CN 102668854A CN 2012101733392 A CN2012101733392 A CN 2012101733392A CN 201210173339 A CN201210173339 A CN 201210173339A CN 102668854 A CN102668854 A CN 102668854A
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Abstract
The invention discloses a cutting propagation method for momordica grosvenori. The method comprises the following operation steps of: using one section of semi-ligneous vine with one leaf as a cutting slip, dipping the stem base of the cutting slip in a root inducer for 30-60 min, taking the stem base of the cutting slip out, airing in the shade until the surface does not drip water, inserting the stem base of the cutting slip in a fine sand seedbed for cultivation, spraying a root growth promoting nutrient agent every 5 days on the same day of cutting and after that day till rooting and seedling, wherein brassinolide and heteroauxin are used as the root inducer for momordica grosvenori, and bacillus subtilis fertilizer and nucleotide are used as the root growth promoting nutrient agent during the culturing process. The cutting slip starts rooting and sprouting after culturing for 17-19 days and are out of seedling and planted in field after 30 days; and the seedling rate is more over 95%. The method provided by the invention can quickly obtain seedlings of the momordica grosvenori, effectively resolves the problems of high producing cost, late fruitage and slow popularization, and offers effective provenance guarantee for the deep development of the medical value of the momordica grosvenori.
Description
Technical field
The present invention relates to plant cuttage breeding field, especially a kind of Momordica grosvenori cuttage breeding method.
Background technology
Momordica grosvenori (Siraitia grosvenorii (Swingle) C.Jeffrey) is a Curcurbitaceae Momordica grosvenori platymiscium, is the medicine-food two-purpose fruit, has the merit of heat-clearing, cough-relieving apophlegmatic.Main product is in Guangxi, and all there is introducing and planting in Guangdong, Hunan, Fujian.Momordica grosvenori contains multiple sweet glycosides, and wherein the mogroside V is non-sugared sweet substance, and sugariness is 300～400 times of sucrose, for having the natural sweetener of health-care efficacy.
Bacillus subtilis has the organic ability of decomposition: when decomposing propagation; Can disengage highly active amylase; The macromolecular substances that difficulty is decomposed resolves into available small-molecule substance, produces abundant metabolism product: materials such as synthetic multiple organic acid, enzyme, physiologically active; Have simultaneously and suppress harmful pathogen growth: can suppress harmful microbe growth and breedings such as harmful bacterium, pathogen.Bacillus subtilis bacterial manure is used on crops such as root-mustard, radish, wheat, and its growth is all had facilitation in various degree.
Nucleotide have induce with the activated plant body in growth and system of defense; Strengthen cell enlargement; Regulate growth and development of plant, strengthen photosynthesis and absorb transformation function; Disease-resistant, antitoxin, the detoxifcation of enhancing crop, degeneration-resistant, cold-resistant, drought resisting, ability resistant to lodging are replenished multiple nutritional components and the required element of growth, promote crop growth, strong sprout.Also has simultaneously good disease-resistant bactericidal activity.
In Momordica grosvenori was produced, prematurity, precocity fruiting can improve Lo Han Guo fruit output and quality.Cultivating and produce Momordica grosvenori seedling high-quality, that yield positive results morning is the thing that the orchard worker dreams of, and also is that Momordica grosvenori plantation development is required.Momordica grosvenori seminal propagation,, male and female plant ratio loaded down with trivial details because of its operation is 3:7, and can't distinguish during seedling and discarded by the orchard worker.In the production, the most ancient propagation method is the potato seed breeding, expands into little potato piece through keeping down the vines of a creeping plant and forms.Continue to use the history of centuries.Drawbacks such as it has: incubation rate is low, potato seed is not of uniform size, and fall ill easily in the plantation back, it is slow to yield positive results have seriously restricted the development of Momordica grosvenori.Momordica grosvenori tissue cultivating seedling breeding is to utilize modern biotechnology, chooses that bud point tissue is cultivated, root induction Cheng Miao again.Exist: apparatus expensive, specification requirement are high, production cost is high, detoxification is incomplete, seedling raise period is long, and it is late to yield positive results, and are prone to cause problems such as virus disease and excessive growth, cause the Momordica grosvenori underproduction, even the mistake receipts.The Momordica grosvenori cottage propagation is short because of its time, genetic character good, be prone to grasp, and cost is low, and the result early etc. is liked by the orchard worker deeply.Exist problems such as many with material, that survival rate is low at present, suppress its application and popularization.Utilize the somatotropin of variety classes and variable concentrations, in the various plants cuttage, succeed.Wherein, used hormone or hormone combinations and concentration thereof are the key problem in technology of cuttage success or not, and hormone and concentration that different plants are used differ widely.In cuttage, mostly pay attention to inserting the initial HORMONE TREATMENT of fringe and the screening of matrix species, in cuttage, normal slotting fringe dehydration, the phenomenon of here withering too early of occurring causes that survival rate is not high, seedling quality difference and to go out the garden time long.Be not reported to inserting the additional effective nutriment of fringe in the cuttage process.
Summary of the invention
The technical problem that the present invention will solve provides all higher Momordica grosvenori cuttage breeding method of a kind of material-saving, rooting rate and planting percent.
The present invention is that to solve the problems of the technologies described above the technical scheme that is adopted following:
A kind of Momordica grosvenori cuttage breeding method of the present invention, its operating procedure is following:
Tendril with the Momordica grosvenori semi-lignified does to insert fringe, will insert the fringe base portion and be soaked in 30min～60min in the root induction agent, pulls out; Dry in the air in the cool and do not drip to the surface; Cuttage is cultivated in the fine sand seedbed, the same day after the cuttage and the short root nutritional agents of later every 5d spray once, until take root, Cheng Miao.
Momordica grosvenori cuttage breeding method of the present invention, described root induction agent contains the material of following concentration:
Brassin lactones 0.8～1.0 mg/L, heteroauxin 50～60mg/kg.This root induction agent is used for cuttage rooting and induces.
Momordica grosvenori cuttage breeding method of the present invention, described short root nutritional agents contains following concentration material:
Fertile 1.0～1.2 ml/L of gemma hay bacillus wherein contain the effective bacterium number of bacillus subtilis>=2 * 10
8It is individual/ml,
Nucleotide 40mg/kg～60mg/ kg.Should be used for passing through Foliage Absorption after the cuttage, the nutriment of supplying with or regulating by short root nutritional agents.
It is that green tendril nutrition to strong green semi-lignified, no damage by disease and insect is complicated and confused that Momordica grosvenori cuttage breeding method of the present invention, said slotting fringe adopt the leaf look, and will to insert that fringe cuts be one to save and be with a leaf, and long 3cm～5cm is a benchmark with the joint, the long 1cm in upper end, the long 2cm～4cm in lower end.
Momordica grosvenori cuttage breeding method of the present invention, said seedbed condition of culture is: 25 ± 2 ℃ of temperature, air humidity 77% ± 3%, shade rate 75～85%.
Momordica grosvenori cuttage breeding method of the present invention, said fine sand seedbed are the river sands that adopted 1mm～1.5mm standard screen, and the river sand ulking thickness is 15cm.
The inventive method adopts brassin lactones (BR)+heteroauxin (IAA) as the agent of Momordica grosvenori root induction; These two kinds of somatotropin combinations; Under suitable concentration, can activate the effect enzyme that Momordica grosvenori is inserted the fringe cells in vivo, promote Momordica grosvenori to insert taking root of fringe and sprout and growth; The present invention also adopts the fertile and short root nutritional agents of nucleotide conduct of gemma hay bacillus, can promote cuttage rooting and the sprouting sprouting of Momordica grosvenori one joint with a leaf effectively, cultivates to begin to take root, sprout sprouting in 17 days to 19 days.Use the inventive method and can obtain the Momordica grosvenori seedling fast, solve effectively that the breeding production cost is high, the result is late and promote slow problem, for deeply developing its pharmaceutical value strong provenance guarantee is provided.
Embodiment
Below in conjunction with embodiment the present invention is done further description.
Embodiment 1
The green tendril of getting Momordica grosvenori semi-lignified, no damage by disease and insect makes to insert fringe; It is cut into the slotting fringe of the joint of the long 3cm in lower end, the long 1cm in upper end with a leaf; It is in the root induction agent that slotting fringe base portion is soaked in the water-soluble of brassinolide-containing 0.8mg/L, heteroauxin 60mg/kg; 30min adopts to join promptly at present at present and uses.Pull out, dry in the air slightly, drop removes surperficial water droplet.Cuttage is on the fine sand seedbed, and the river sand of 1.5mm standard screen was adopted in the seedbed, and the river sand ulking thickness is 15cm; Insert the fringe mounted blade, not overlapping, reach the short root nutritional agents of every 5d spray after the cuttage once, connect spray with 6 times, the short root nutritional agents of employing contains gemma hay bacillus fertilizer 1.0 ml/L and (contains the effective bacterium number of bacillus subtilis>=2 * 10
8Individual/ml), nucleotide 40mg/ kg.In temperature is 25 ℃, and air humidity 77%% under the condition of shade rate 80%, was cultivated 17 days, and slotting fringe begins to take root, sprout sprouting, cuttage 30d statistics, and rooting rate is 98%, planting percent is 98%, newborn robust plant, green, root is sturdy, and is many from inserting the mitogenetic one-level root of fringe.
Embodiment 2
The strong green tendril of getting Momordica grosvenori semi-lignified, no damage by disease and insect makes to insert fringe; It is cut into the slotting fringe of the joint of the long 4cm in lower end, the long 1cm in upper end with a leaf; It is in the root induction agent that slotting fringe base portion is soaked in the water-soluble of brassinolide-containing 1.0 mg/L, heteroauxin 40mg/kg; 45min adopts promptly at present and uses.Pull out, dry in the air slightly, drop removes surperficial water droplet.Cuttage is on the fine sand seedbed, and the river sand of 1.2mm standard screen was adopted in the seedbed, and the river sand ulking thickness is 15cm; Insert the fringe mounted blade, not overlapping, reach the short root nutritional agents of every 5d spray after the cuttage once, connect spray with 6 times, the short root nutritional agents of employing contains gemma hay bacillus fertilizer 1.1 ml/L and (contains the effective bacterium number of bacillus subtilis>=2 * 10
8Individual/ml), nucleotide 50mg/ kg.In temperature is 27 ℃, and air humidity 80% under the condition of shade rate 85%, was cultivated 19 days; Slotting fringe begins to take root, sprout sprouting, cuttage 30d statistics, and rooting rate 97% is 96% for planting percent, newborn plant is more sturdy; Green, root is thinner, and mitogenetic secondary root is more, and it is moderate to grow.
Embodiment 3
Get the green tendril of Momordica grosvenori semi-lignified, no damage by disease and insect; It is cut into the long 4cm in lower end, and the joint of the long 1cm in upper end is with the slotting fringe of a leaf, and it is in the root induction agent that slotting fringe base portion is soaked in the water-soluble of brassinolide-containing 0.9 mg/L, heteroauxin 50mg/kg; 60min adopts promptly at present and uses.Pull out, dry in the air slightly, drop removes surperficial water droplet.Cuttage is on the fine sand seedbed, and the river sand of 1mm standard screen was adopted in the seedbed, and the river sand ulking thickness is 15cm; Insert the fringe mounted blade, not overlapping, reach the short root nutritional agents of every 5d spray after the cuttage once, connect spray with 6 times, the short root nutritional agents of employing contains gemma hay bacillus fertilizer 1.2 ml/L and (contains the effective bacterium number of bacillus subtilis>=2 * 10
8Individual/ml), nucleotide 60mg/ kg.In temperature is 23 ℃, and air humidity 75% under the condition of shade rate 78%, was cultivated 19 days, and slotting fringe begins to take root, germinate; Cuttage 30d statistics, rooting rate is 96%, and planting percent is 95%, and newborn plant is more sturdy; Green, internode is longer, and root is thick, long, and one-level root and secondary root are all more.
Claims (6)
1. a Momordica grosvenori cuttage breeding method is characterized in that, its operating procedure is following:
Tendril with the Momordica grosvenori semi-lignified does to insert fringe, will insert the fringe base portion and be soaked in 30min～60min in the root induction agent, pulls out; Dry in the air in the cool and do not drip to the surface; Cuttage is cultivated in the fine sand seedbed, the same day after the cuttage and the short root nutritional agents of later every 5d spray once, until take root, Cheng Miao.
2. according to the said Momordica grosvenori cuttage breeding method of claim 1, it is characterized in that said root induction agent contains the material of following concentration:
Brassin lactones 0.8～1.0 mg/L, heteroauxin 50～60mg/kg.
3. according to the said Momordica grosvenori cuttage breeding method of claim 1, it is characterized in that said short root nutritional agents contains following concentration material:
Fertile 1.0～1.2 ml/L of gemma hay bacillus wherein contain the effective bacterium number of bacillus subtilis>=2 * 10
8It is individual/ml,
Nucleotide 40mg/kg～60mg/kg.
4. according to the said Momordica grosvenori cuttage breeding method of claim 1, it is characterized in that it is green nutrition branches and tendrils to strong green semi-lignified, no damage by disease and insect that said slotting fringe adopts the leaf look; And will insert fringe cut be one the joint be with a leaf, long 3cm～5cm is a benchmark with the joint; The long 1cm in upper end, the long 2cm～4cm in lower end.
5. according to the said Momordica grosvenori cuttage breeding method of claim 1, it is characterized in that said seedbed condition of culture is: 25 ± 2 ℃ of temperature, air humidity 77% ± 3%, shade rate 75-85%.
6. according to the said Momordica grosvenori cuttage breeding method of claim 1, it is characterized in that said fine sand seedbed is the river sand that adopted 1mm～1.5mm standard screen, the river sand ulking thickness is 15cm.
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102919045A (en) * | 2012-11-22 | 2013-02-13 | 季永飞 | Fructus momordicae high-yield cultivation technology allowing fructus momordicae to be planted one time and harvested two times in year |
| CN103772060A (en) * | 2014-02-20 | 2014-05-07 | 广西壮族自治区药用植物园 | Inducer for enlarging and promoting sweetness of siraitia grosvenorii and application method thereof |
| CN104969806A (en) * | 2015-07-10 | 2015-10-14 | 吴伦忠 | Momordica grosvenori root-knot nematode integrated control method |
| CN104996146A (en) * | 2015-07-27 | 2015-10-28 | 广西鹿寨方田贸易有限公司 | Momordica grosvenori seedling growing method |
| CN105532234A (en) * | 2015-12-25 | 2016-05-04 | 蒙奇同 | Method for cultivating red-pulp hylocereus polyrhizus |
| CN106069348A (en) * | 2016-07-29 | 2016-11-09 | 桂林实力科技有限公司 | The method for culturing seedlings of Fructus Momordicae |
| CN106561425A (en) * | 2016-10-31 | 2017-04-19 | 广西壮族自治区药用植物园 | Cutting propagation method for kmeria septentrionalis |
| CN106718498A (en) * | 2016-11-24 | 2017-05-31 | 枞阳县弘阳园林绿化有限公司 | A kind of cuttage and seedling culture method of cuckoo |
| CN106747907A (en) * | 2016-11-18 | 2017-05-31 | 钟山县橙浩水果种植专业合作社 | A kind of tribute mandarin orange nursery dedicated set compound |
| CN107173125A (en) * | 2017-06-09 | 2017-09-19 | 柳州市跃坤农业开发有限公司 | The implantation methods of Momordica grosvenori |
| CN107484526A (en) * | 2017-08-31 | 2017-12-19 | 秦福玉 | A kind of method of Momordica grosvenori cuttage and seedling culture |
| CN107493888A (en) * | 2017-08-31 | 2017-12-22 | 秦福玉 | Momordica grosvenori cuttage breeding method |
| CN107668037A (en) * | 2016-08-01 | 2018-02-09 | 江苏龙灯化学有限公司 | A kind of plant growth regulator composition |
| CN111296451A (en) * | 2020-04-07 | 2020-06-19 | 江西瑞博特生物科技有限公司 | Rooting agent for cutting seedling as well as preparation method and application thereof |
| CN112136615A (en) * | 2020-09-27 | 2020-12-29 | 湖南雨泉农业发展有限公司 | High-yield fructus momordicae planting method |
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Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102919045A (en) * | 2012-11-22 | 2013-02-13 | 季永飞 | Fructus momordicae high-yield cultivation technology allowing fructus momordicae to be planted one time and harvested two times in year |
| CN103772060A (en) * | 2014-02-20 | 2014-05-07 | 广西壮族自治区药用植物园 | Inducer for enlarging and promoting sweetness of siraitia grosvenorii and application method thereof |
| CN104969806A (en) * | 2015-07-10 | 2015-10-14 | 吴伦忠 | Momordica grosvenori root-knot nematode integrated control method |
| CN104996146A (en) * | 2015-07-27 | 2015-10-28 | 广西鹿寨方田贸易有限公司 | Momordica grosvenori seedling growing method |
| CN105532234A (en) * | 2015-12-25 | 2016-05-04 | 蒙奇同 | Method for cultivating red-pulp hylocereus polyrhizus |
| CN105532234B (en) * | 2015-12-25 | 2018-07-10 | 蒙奇同 | The breeding method of red heart Hylocereus undatus |
| CN106069348A (en) * | 2016-07-29 | 2016-11-09 | 桂林实力科技有限公司 | The method for culturing seedlings of Fructus Momordicae |
| CN107668037A (en) * | 2016-08-01 | 2018-02-09 | 江苏龙灯化学有限公司 | A kind of plant growth regulator composition |
| CN107668037B (en) * | 2016-08-01 | 2022-11-18 | 江苏龙灯化学有限公司 | Plant growth regulator composition |
| CN106561425A (en) * | 2016-10-31 | 2017-04-19 | 广西壮族自治区药用植物园 | Cutting propagation method for kmeria septentrionalis |
| CN106747907A (en) * | 2016-11-18 | 2017-05-31 | 钟山县橙浩水果种植专业合作社 | A kind of tribute mandarin orange nursery dedicated set compound |
| CN106718498A (en) * | 2016-11-24 | 2017-05-31 | 枞阳县弘阳园林绿化有限公司 | A kind of cuttage and seedling culture method of cuckoo |
| CN107173125A (en) * | 2017-06-09 | 2017-09-19 | 柳州市跃坤农业开发有限公司 | The implantation methods of Momordica grosvenori |
| CN107484526A (en) * | 2017-08-31 | 2017-12-19 | 秦福玉 | A kind of method of Momordica grosvenori cuttage and seedling culture |
| CN107493888A (en) * | 2017-08-31 | 2017-12-22 | 秦福玉 | Momordica grosvenori cuttage breeding method |
| CN111296451A (en) * | 2020-04-07 | 2020-06-19 | 江西瑞博特生物科技有限公司 | Rooting agent for cutting seedling as well as preparation method and application thereof |
| CN111296451B (en) * | 2020-04-07 | 2021-07-13 | 江西瑞博特生物科技有限公司 | Rooting agent for cutting seedling as well as preparation method and application thereof |
| CN112136615A (en) * | 2020-09-27 | 2020-12-29 | 湖南雨泉农业发展有限公司 | High-yield fructus momordicae planting method |
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Application publication date: 20120919 |