CN111296451B - Rooting agent for cutting seedling as well as preparation method and application thereof - Google Patents
Rooting agent for cutting seedling as well as preparation method and application thereof Download PDFInfo
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- CN111296451B CN111296451B CN202010264784.4A CN202010264784A CN111296451B CN 111296451 B CN111296451 B CN 111296451B CN 202010264784 A CN202010264784 A CN 202010264784A CN 111296451 B CN111296451 B CN 111296451B
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- auxin
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- rooting agent
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- cutting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N39/00—Biocides, pest repellants or attractants, or plant growth regulators containing aryloxy- or arylthio-aliphatic or cycloaliphatic compounds, containing the group or, e.g. phenoxyethylamine, phenylthio-acetonitrile, phenoxyacetone
- A01N39/02—Aryloxy-carboxylic acids; Derivatives thereof
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- A—HUMAN NECESSITIES
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- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/56—1,2-Diazoles; Hydrogenated 1,2-diazoles
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/12—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, neither directly attached to a ring nor the nitrogen atom being a member of a heterocyclic ring
- A01N47/14—Di-thio analogues thereof
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
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Abstract
The invention is applicable to the technical field of plant cultivation, and provides a rooting agent for cutting seedling as well as a preparation method and an application thereof, wherein the rooting agent comprises the following components in parts by weight: 1 to 99 parts of indole auxin, 1 to 99 parts of naphthoic acid auxin, 0.0001 to 0.0015 part of steroid auxin, 0.1 to 9.9 parts of nutrient auxin and 0.1 to 9.9 parts of thiram bactericide. The rooting agent provided by the invention is simple in raw materials, convenient to prepare, broad-spectrum and efficient in use, has the characteristics of quick rooting, multiple rooting, corrosion and disease resistance and good seedling rate, has the function of inducing the plant cutting callus and cortex on the upper part of a cut to jointly generate roots, is a quick-dipping type plant rooting agent, and can be widely applied to the fields of breeding, transplanting, protecting and the like of agriculture, forestry, gardening, gardens, Chinese herbal medicines and rare or endangered rare and precious plants.
Description
Technical Field
The invention belongs to the technical field of plant cultivation, and particularly relates to a rooting agent for cutting seedling as well as a preparation method and application thereof.
Background
Cutting propagation refers to a propagation method of cutting branches, leaves or a part of roots of plants, inserting the branches, leaves or parts of the roots into a matrix, and enabling the branches, leaves or parts to root, sprout, shoot and grow into new plants. Cuttage is a traditional plant asexual propagation technology, is only used as a propagation means of plants easy to grow roots initially, and through development of many years, the cuttage technology is mature day by day, the application range is wider and wider, and a lot of flowers and trees, fruit trees and economic trees mainly depend on cuttage propagation. In order to improve the survival rate of cuttage, people test the cuttage in various ways, such as leaf cutting, leaf bud cutting, root cutting, twig cutting and hardwood cutting; in the aspect of promoting the cuttage propagation of plants to root, people try a great deal of application of various rooting materials.
In order to solve the problem of rooting of the plant cutting, the prior art mainly adopts various rooting agents sold in the market and various published patents of the rooting agents to achieve the purpose of rooting the cutting. For example, ABT rooting powder sold in the market No. 1-5 (China Lin Ke Ching), Guogong heel (Sichuan Guoguang chemical company), and Genbao No. 1-3 (Shanxi nongda); and the patent publications of the rooting agent are also more.
The rooting agent is evaluated from component design and formula auxiliary agents, the rooting agent adopts a hormone-type component formula or a nutritional component formula or a formula concept of extracting specific functional components from natural plants, although various market commodities and invention patents of the plant rooting agent for inducing the plant to root are numerous, the rooting agent generally has certain specificity and limitation, so that the limitation of application effect or the bias of target plants inevitably exists; although some of the dye baths have a wider application range and a poor treatment effect, the dye baths often need to wait for several hours to tens of hours, which brings practical inconvenience to application or production. For example, from the aspect of producers in the seedling culture industry, higher production benefits are demanded, dipping time is often shortened, and the most popular method is the most convenient and rapid rooting agent dipping method, namely a cutting slip rapid dipping method, but the rapid dipping method is not stable in dealing with the cutting propagation of plants difficult to root, and when the rooting agent is used for cutting treatment, the effect of forming adventitious roots on cutting slips of the plants difficult to root is often unstable; the phenomena of low rooting survival rate, even no root formation, rot of cutting slips and the like are caused, and when the cutting slips are used for rooting in humid and high-temperature climates, the cutting slips are rotten after the roots are not grown. The most typical example is represented by tender branch cuttage of difficultly rooted tree species such as masson pine, phoebe bournei and maple, and the treatment of the quick dipping method by adopting the rooting agent is not successful.
On the other hand, although the rooting powder or the prior art has a certain effect on rooting of the plant cutting, the meristem of the plant cutting, in particular, the parenchyma cell group with a special cambium development, which has formed or has not formed a root primitive body (root primitive group), smoothly undergoes dedifferentiation and redifferentiation into a root primitive body in a blunt cone shape through cell division, invades into the phloem, has no inducing effect on rooting of the bark part leading to the bark hole, has almost no formation period of the callus or adventitious root of the plant cutting, can synchronously root the bark part at the lower end of the cutting and the cortex at the upper part of the incision, and has no effect on rooting of the root part and the callus together.
In summary, the rooting agent is a very important factor for influencing the cutting rooting of the plants, and although the rooting agent products and invention patents of various material components for inducing the plants to root in the prior art are many, the rooting agent generally has certain specificity and limitation. Therefore, the research of the rooting agent with quick rooting, multiple rooting, rot resistance, germ resistance, high efficiency and broad spectrum becomes a hotspot of the research of the rooting agent at present, and the urgent need is broken through.
Disclosure of Invention
The embodiment of the invention aims to provide a rooting agent for raising seedlings by cutting, aiming at solving the problems in the background art.
The embodiment of the invention is realized in such a way that the rooting agent for cutting seedling comprises the following components in parts by weight: 1 to 99 parts of indole auxin, 1 to 99 parts of naphthoic acid auxin, 0.0001 to 0.0015 part of steroid auxin, 0.1 to 9.9 parts of nutrient auxin and 0.1 to 9.9 parts of thiram bactericide.
As a preferable scheme of the embodiment of the invention, the rooting agent comprises the following components in parts by weight: 30 to 79 portions of indole auxin, 35 to 75 portions of naphthoic auxin, 0.0003 to 0.0013 portion of steroid auxin, 0.2 to 5.5 portions of nutrient auxin and 0.5 to 6.5 portions of thiram bactericide.
As another preferable embodiment of the present invention, the indole auxin is one or more of indole acetic acid, indole butyric acid and indole ripester; the naphthoic auxin is one or more of naphthoxyacetic acid, naphthylacetic acid and methyl naphthylacetate.
As another preferred embodiment of the present invention, the steroid auxin is one or more of natural brassinolide, phytosterol, steroid saponin and steroid alkaloid.
As another preferable scheme of the embodiment of the invention, the nutrient auxin is one or more of glycine, leucine, chitosan, amino-oligosaccharide, carboxymethyl chitosan and lentinan.
As another preferable mode of the embodiment of the present invention, the thiram-based bactericide is one or more of thiram, arsine, ziram, ferbam, nickel, arsine, ammonium, and sodium.
Another object of the embodiments of the present invention is to provide a method for preparing the above rooting agent, which comprises the following steps:
weighing indole auxin, naphthoic acid auxin, steroid auxin, nutritional auxin and thiram bactericide according to the weight parts of the components for later use;
the indole auxin, the naphthoic auxin, the steroid auxin, the nutritional auxin and the thiram bactericide are crushed and mixed to obtain the rooting agent.
Another object of the embodiments of the present invention is to provide a hair root agent prepared by the above preparation method.
The other purpose of the embodiment of the invention is to provide an application of the rooting agent in inducing the cutting shoot of the plant to take root.
As another preferable solution of the embodiment of the present invention, the application includes the steps of:
mixing the rooting agent with an ethanol solution with the volume concentration of 50-95% to obtain a rooting agent base solution; the mass ratio of the rooting agent to the ethanol solution is 1 (40-60);
diluting the rooting agent base solution with water to obtain a rooting agent diluent; the mass ratio of the rooting agent base liquid to water is 1 (199-999);
and carrying out quick dipping treatment on the plant cutting shoot by using the rooting agent diluent.
Wherein, the indole auxin can remarkably promote the adventitious root generation induced by the plant cutting.
The naphthoic auxin has small inducing effect on adventitious roots and can obviously promote the growth and elongation of new roots.
One of the phytosterols with higher biological activity is brassinolide, which can be divided into natural brassinolide and chemically synthesized brassinolide from the production process, wherein the natural brassinolide is a plant extract, and the chemically synthesized brassinolide is an industrial synthetic product. The natural brassinolide is a newly discovered steroid hormone, and can promote the elongation and division of cells, enhance the stress resistance of the cells, promote the growth of plants and strengthen the yield of plant nutrients.
Glycine, leucine and other amino acid components can provide nutrients and regulate physiological function, raise crop resistance and promote crop growth.
The broad-spectrum protective Fumei series bactericide has better control range relative to various crops such as downy mildew, epidemic disease, anthracnose, root rot and seedling yellow blight. Has important functions of enhancing the cell vitality and disease resistance of plant cuttings, protecting incision tissues from being infected by pathogenic bacteria and inducing hairy roots.
The rooting agent for cuttage seedling raising provided by the embodiment of the invention has the advantages of simple raw materials, convenience in preparation, broad spectrum and high efficiency in use, has the characteristics of quick rooting, multiple rooting, corrosion and disease resistance and good seedling rate, has the function of inducing the plant cutting callus and cortex on the upper part of a cut to jointly generate roots, is a quick-dipping type plant rooting agent, and can be widely applied to the fields of agriculture, forestry, gardening, gardens, breeding, transplanting and protection of Chinese herbal medicines and rare or endangered rare plants and the like.
Compared with the prior art, the invention has the beneficial effects that:
(1) the rooting agent provided by the embodiment of the invention breaks through the limitation that single rooting exists in callus (or skin hole) in the prior art, can effectively induce the plant cutting callus and the upper cortex of the cut to jointly generate roots, and realizes the consistency of multiple rooting of one cutting.
(2) The bactericide component contained in the rooting agent provided by the embodiment of the invention effectively reduces bacterial and fungal infection in the whole process of cutting and seedling formation of the cutting slips.
(3) The nutritional auxin contained in the rooting agent provided by the embodiment of the invention effectively improves the rooting effect, and can ensure that the plant cutting shoots and roots are quick, the rooting and cutting shoots are more, and the number of root hairs can reach 15-55.
(4) By using the rooting agent provided by the embodiment of the invention, the dipping time required by plant cutting shrinkage is extremely short, the plant cutting shrinkage can be dipped along with the cutting, waiting is not needed, the rooting agent is convenient and quick, the production cost is reduced, and the working efficiency is improved.
(5) The rooting agent provided by the embodiment of the invention is effective for transplanting various large trees, shrubs and herbaceous plants which are difficult to root, and is suitable for cuttage or transplantation of seedlings of various economic forests, Chinese herbal medicines, fruit trees, vegetables and the like.
Drawings
Fig. 1 is a rooting effect diagram of the camellia oleifera cutting slips obtained in example 13 of the present invention.
FIG. 2 is a drawing showing the rooting effect of the cutting of the Camellia nitidissima Hayata obtained in example 14 of the present invention.
FIG. 3 is a graph showing the rooting effect of the rose damascena cutting obtained in example 15 of the present invention.
FIG. 4 is a graph showing the rooting effect of the Broussonetia papyrifera cutting shoot obtained in example 16 of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Example 1
The embodiment provides a rooting agent for raising seedlings by cutting, and the preparation method comprises the following steps:
s1, weighing 10g of indole auxin, 990g of naphthoic auxin, 0.001g of steroid auxin, 1g of nutritional auxin and 1g of thiram bactericide for later use. Wherein the indole auxin is indoleacetic acid; the naphthoic acid auxin is naphthoxyacetic acid; the steroid auxin is phytosterol; the nutrient auxin is glycine; the thiram series bactericide is thiram.
S2, crushing and mixing the indole auxin, the naphthoic auxin, the steroid auxin, the nutritional auxin and the thiram bactericide according to the conventional method to obtain the rooting agent.
Example 2
The embodiment provides a rooting agent for raising seedlings by cutting, and the preparation method comprises the following steps:
s1, 990g of indole auxin, 10g of naphthoic auxin, 0.015g of steroid auxin, 99g of nutritional auxin and 99g of thiram bactericide are weighed for later use. Wherein the indole auxin is a mixture of indole butyric acid and indole ester; the naphthoic auxin is a mixture of naphthylacetic acid and methyl naphthylacetate; the steroid auxin is a mixture of steroid saponin and steroid alkaloid; the nutrient auxin is mixture of leucine and lentinan; the thiram series bactericide is a mixture of thiram and thiram sodium.
S2, crushing and mixing the indole auxin, the naphthoic auxin, the steroid auxin, the nutritional auxin and the thiram bactericide according to the conventional method to obtain the rooting agent.
Example 3
The embodiment provides a rooting agent for raising seedlings by cutting, and the preparation method comprises the following steps:
s1, weighing 300g of indole auxin, 750g of naphthoic auxin, 0.003g of steroid auxin, 2g of nutrient auxin and 5g of thiram bactericide for later use. Wherein the indole auxin is a mixture of indoleacetic acid, indolebutyric acid and indole ester; the naphthoic auxin is a mixture of naphthoxyacetic acid, naphthylacetic acid and methyl naphthylacetate; the steroid auxin is a mixture of phytosterol, steroid saponin and steroid alkaloid; the nutrient auxin is a mixture of chitosan, amino-oligosaccharin and carboxymethyl chitosan; the thiram series bactericide is a mixture of thiram zinc, thiram iron and thiram nickel.
S2, crushing and mixing the indole auxin, the naphthoic auxin, the steroid auxin, the nutritional auxin and the thiram bactericide according to the conventional method to obtain the rooting agent.
Example 4
The embodiment provides a rooting agent for raising seedlings by cutting, and the preparation method comprises the following steps:
s1, weighing 790g of indole auxin, 350g of naphthoic auxin, 0.013g of steroid auxin, 55g of nutrient auxin and 65g of thiram bactericide for later use. Wherein the indole auxin is an indole ester; the naphthoic auxin is methyl naphthylacetate; the steroid auxin is steroid alkaloid; the nutrient auxin is a mixture of glycine and leucine; the thiram series bactericide is thiram.
S2, crushing and mixing the indole auxin, the naphthoic auxin, the steroid auxin, the nutritional auxin and the thiram bactericide according to the conventional method to obtain the rooting agent.
Example 5
The embodiment provides a rooting agent for raising seedlings by cutting, and the preparation method comprises the following steps:
s1, weighing 300g of indole auxin, 690g of naphthoic auxin, 0.005g of steroid auxin, 2g of nutritional auxin and 5g of thiram bactericide for later use. Wherein the indole auxin is indolebutyric acid; the naphthoic auxin is naphthylacetic acid; the steroid auxin is natural brassinolide; the nutrient auxin is amino-oligosaccharide; the thiram series bactericide is thiram.
S2, crushing and mixing the indole auxin, the naphthoic auxin, the steroid auxin, the nutritional auxin and the thiram bactericide according to the conventional method to obtain the rooting agent.
Example 6
The embodiment provides a rooting agent for raising seedlings by cutting, and the preparation method comprises the following steps:
s1, weighing 600g of indole auxin, 390g of naphthoic auxin, 0.012g of steroid auxin, 25g of nutritional auxin and 55g of thiram bactericide for later use. Wherein the indole auxin is indolebutyric acid; the naphthoic auxin is naphthylacetic acid; the steroid auxin is natural brassinolide; the nutrient auxin is amino-oligosaccharide; the thiram series bactericide is thiram.
S2, crushing and mixing the indole auxin, the naphthoic auxin, the steroid auxin, the nutritional auxin and the thiram bactericide according to the conventional method to obtain the rooting agent.
Example 7
The embodiment provides a rooting agent for raising seedlings by cutting, and the preparation method comprises the following steps:
s1, weighing 390g of indole auxin, 650g of naphthoic auxin, 0.003g of steroid auxin, 3g of nutrient auxin and 5g of thiram bactericide for later use. Wherein the indole auxin is indolebutyric acid; the naphthoic auxin is naphthylacetic acid; the steroid auxin is natural brassinolide; the nutrient auxin is amino-oligosaccharide; the thiram series bactericide is thiram.
S2, crushing and mixing the indole auxin, the naphthoic auxin, the steroid auxin, the nutritional auxin and the thiram bactericide according to the conventional method to obtain the rooting agent.
Example 8
The embodiment provides a rooting agent for raising seedlings by cutting, and the preparation method comprises the following steps:
s1, 690g of indole auxin, 350g of naphthoic auxin, 0.011g of steroid auxin, 35g of nutrient auxin and 55g of thiram bactericide are weighed for later use. Wherein the indole auxin is indolebutyric acid; the naphthoic auxin is naphthylacetic acid; the steroid auxin is natural brassinolide; the nutrient auxin is amino-oligosaccharide; the thiram series bactericide is thiram.
S2, crushing and mixing the indole auxin, the naphthoic auxin, the steroid auxin, the nutritional auxin and the thiram bactericide according to the conventional method to obtain the rooting agent.
Example 9
The embodiment provides a rooting agent for raising seedlings by cutting, and the preparation method comprises the following steps:
s1, weighing 590g of indole auxin, 750g of naphthoic auxin, 0.005g of steroid auxin, 3.5g of nutrient auxin and 6g of thiram bactericide for later use. Wherein the indole auxin is indolebutyric acid; the naphthoic auxin is naphthylacetic acid; the steroid auxin is natural brassinolide; the nutrient auxin is amino-oligosaccharide; the thiram series bactericide is thiram.
S2, crushing and mixing the indole auxin, the naphthoic auxin, the steroid auxin, the nutritional auxin and the thiram bactericide according to the conventional method to obtain the rooting agent.
Example 10
The embodiment provides a rooting agent for raising seedlings by cutting, and the preparation method comprises the following steps:
s1, weighing 790g of indole auxin, 450g of naphthoic auxin, 0.013g of steroid auxin, 45g of nutrient auxin and 65g of thiram bactericide for later use. Wherein the indole auxin is indolebutyric acid; the naphthoic auxin is naphthylacetic acid; the steroid auxin is natural brassinolide; the nutrient auxin is amino-oligosaccharide; the thiram series bactericide is thiram.
S2, crushing and mixing the indole auxin, the naphthoic auxin, the steroid auxin, the nutritional auxin and the thiram bactericide according to the conventional method to obtain the rooting agent.
Example 11
The embodiment provides a rooting agent for raising seedlings by cutting, and the preparation method comprises the following steps:
s1, weighing 350g of indole auxin, 600g of naphthoic auxin, 0.006g of steroid auxin, 5.5g of nutrient auxin and 8g of thiram bactericide for later use. Wherein the indole auxin is indolebutyric acid; the naphthoic auxin is naphthylacetic acid; the steroid auxin is natural brassinolide; the nutrient auxin is amino-oligosaccharide; the thiram series bactericide is thiram.
S2, crushing and mixing the indole auxin, the naphthoic auxin, the steroid auxin, the nutritional auxin and the thiram bactericide according to the conventional method to obtain the rooting agent.
Example 12
The embodiment provides a rooting agent for raising seedlings by cutting, and the preparation method comprises the following steps:
s1, weighing 550g of indole auxin, 400g of naphthoic auxin, 0.015g of steroid auxin, 55g of nutritional auxin and 85g of thiram bactericide for later use. Wherein the indole auxin is indolebutyric acid; the naphthoic auxin is naphthylacetic acid; the steroid auxin is natural brassinolide; the nutrient auxin is amino-oligosaccharide; the thiram series bactericide is thiram.
S2, crushing and mixing the indole auxin, the naphthoic auxin, the steroid auxin, the nutritional auxin and the thiram bactericide according to the conventional method to obtain the rooting agent.
Example 13
The embodiment provides an application of the rooting agent provided in the embodiment 5 in cutting seedling raising of camellia oleifera, which comprises the following steps:
s1, mixing the rooting agent provided in the above example 5 with a 50% ethanol solution in a volume concentration ratio of 1:40 by mass to obtain a rooting agent base solution.
And S2, mixing and diluting the rooting agent base solution and purified water according to the mass ratio of 1:199 to obtain a rooting agent diluent for later use.
S3, recovering the annual semi-lignified branches of the woody plant camellia chebula which is difficult to root with leaves, and placing the branches in an indoor water pool to absorb water for fresh keeping; then, cutting the cut shoots into 10-15 cm long cuttings, reserving 1-3 complete leaves at the upper ends of the cuttings, after the cuttings are cut, soaking the cuttings for 30 minutes for sterilization by using 0.2% potassium permanganate disinfectant, taking the soaked cuttings, placing the cuttings at a shady place for drying moisture in the air, tying the cuttings into bundles according to the standard of 30 or 50 cuttings after the moisture on the surface is completely dried, and taking care to ensure that the lower ends of the cuttings are neat; then, immersing the stems with the base part of the whole bundle of cutting slips not less than 3 centimeters into the prepared rooting agent diluent for quick dipping treatment for 5 seconds in the direction of the cut at the lower end; taking out the cutting and breeding on a disinfected cutting bed provided with a full-light automatic intermittent spraying device.
The rooting agent and the quick dipping method are used for treating the cutting slips of the camellia oleifera which is the plant difficult to root, and the cutting slips are subjected to rooting application or test of not less than 200 ten thousand plants, and 6 commercially available plant rooting hormones are synchronously used in the cutting slip rooting treatment process, so that the results show that the rooting agent provided by the embodiment 5 of the invention has a more obvious effect and is superior to the 6 commercially available rooting hormones. The rooting effect graph of the camellia oleifera cutting shoot using the rooting agent provided by the embodiment 5 of the invention is shown in the attached drawing 1, the rooting survival rate averagely reaches 96.6%, adventitious roots begin to occur in 27-33 days after the camellia oleifera cutting shoot is cut, the vegetative roots of the cutting shoot reach the length of 1.6-3.5 centimeters in 49 days, the number of root hairs reaches 20-55, and the camellia oleifera cutting shoot callus and skin pores can be induced to root together.
Example 14
The embodiment provides an application of the rooting agent provided in the embodiment 7 in cutting seedling raising of the camellia nitidissima, which comprises the following steps:
s1, mixing the rooting agent provided in example 7 with a 55% ethanol solution in a volume concentration ratio of 1:50 by mass to obtain a rooting agent base solution.
S2, mixing and diluting the rooting agent base liquid and purified water according to the mass ratio of 1:600 to obtain a rooting agent diluent for later use.
S3, taking back the annual semi-lignified branches of the woody notched Camellia nitidissima which is difficult to root with leaves, and placing the branches in an indoor water pool to absorb water for preservation; then, cutting the cut slips into 9-12 cm long cuttings, reserving 1 complete leaf at the upper end of each cutting slip, after the cutting of the cutting slips is finished, soaking the cutting slips for 50 minutes for sterilization by using 0.3% potassium permanganate disinfectant, taking out the soaked cutting slips, placing the cut slips at a shading position for air drying, and tying the cutting slips into bundles according to the standard of 10 or 30 pieces of cut slips after the surface moisture is completely dried, wherein care must be taken to ensure that the lower ends of the cutting slips are neat; then, immersing the stems of which the base parts are not less than 3 centimeters of the whole bundle of cutting slips into the prepared rooting agent diluent in the direction of the cut at the lower end for quick dipping for 3 seconds; taking out the cutting and breeding on a disinfected cutting bed provided with a full-light automatic intermittent spraying device.
The rooting agent and the quick dipping method are used for treating the cutting slips of the plant Lumai camellia nitidissima difficult to root, and after not less than 50 ten thousand plants of cutting implementation application or experiments, 5 commercially available plant rooting hormones are synchronously used in the cutting slip rooting treatment process, and the results show that the rooting agent provided by the embodiment 7 of the invention has a more obvious effect and is superior to the 5 commercially available rooting hormones. The rooting effect diagram of the Lumai golden camellia using the rooting agent provided by the embodiment 7 of the invention is shown in the attached figure 2, the rooting survival rate averagely reaches 97.5%, adventitious roots begin to occur in the 25 th to 30 th days after cutting of the Lumai golden camellia cutting slips, the nutritional roots of the cutting slips reach the length of 1.1 to 2.1 centimeters in the 45 th day, the number of root hairs reaches 15 to 30, and the Lumai golden camellia cutting slips can be induced to have roots together with skin pores.
Example 15
The embodiment provides an application of the rooting agent provided in the embodiment 9 in cutting seedling of rosa damascena, which comprises the following steps:
s1, mixing the rooting agent provided in example 9 with a 95% ethanol solution in a volume concentration ratio of 1:60 by mass to obtain a rooting agent base solution.
And S2, mixing and diluting the rooting agent base solution and purified water according to the mass ratio of 1:800 to obtain a rooting agent diluent for later use.
S3, taking back the annual semi-lignified branch of the woody plant Damascus rose which is difficult to root with leaves, and placing the branch in an indoor water pool to absorb water for fresh keeping; cutting the cut slips into 12-18 cm long cuttings, reserving 3-4 complete leaves at the upper ends of the cuttings, after the cuttings are cut, soaking the cuttings for 40 minutes for sterilization by using 0.3% potassium permanganate disinfectant, taking the soaked cuttings, placing the cuttings at a shady place for drying moisture in the air, tying the cuttings into bundles according to the standard of 10 or 30 roots after the moisture on the surface of the cuttings is completely dried, and taking care to ensure that the lower ends of the cuttings are neat; then, immersing the stems of which the base parts are not less than 3 centimeters of the whole bundle of cutting slips into the prepared rooting agent diluent in the direction of the cut at the lower end for quick dipping for 3 seconds; taking out the cutting and breeding on a disinfected cutting bed provided with a full-light automatic intermittent spraying device.
The rooting agent and the quick dipping method are used for treating the cutting slips of the damascus roses which are difficult to root, and after not less than 50 thousands of plants are subjected to cutting implementation application or experiments, 5 commercially available plant rooting hormones are synchronously used in the cutting slip rooting treatment process, and the results show that the rooting agent provided by the embodiment 9 of the invention has a more obvious effect and is superior to the 5 commercially available rooting hormones. The rooting effect graph of the rosa damascena cutting shoot using the rooting agent provided by the embodiment 9 of the invention is shown in the attached drawing 3, the rooting survival rate of the rosa damascena cutting shoot averagely reaches 97.5%, adventitious roots begin to occur 25-30 days after the rosa damascena cutting shoot is cut, the nutritional roots of the cutting shoot reach the length of 2.1-3.8 centimeters on 46 days, the number of root hairs reaches 25-35, and the callus and skin pores of the rosa damascena cutting shoot can be induced to jointly generate roots.
Example 16
The embodiment provides an application of the rooting agent provided in the embodiment 11 in paper mulberry cutting seedling, which includes the following steps:
s1, the rooting agent provided in example 11 was mixed with a 55% ethanol solution in a volume concentration ratio of 1:50 by mass to obtain a rooting agent base solution.
And S2, mixing and diluting the rooting agent base solution and purified water according to the mass ratio of 1:999 to obtain a rooting agent diluent for later use.
S3, recovering the annual semi-lignified branches with leaves of the paper mulberry, and placing the branches in an indoor water pool to absorb water for fresh keeping; then, cutting the cut slips into cut slips with the length of 15-20 cm, keeping 3-4 complete leaves at the upper ends of the cut slips, after the cut slips are cut, soaking the cut slips for 30 minutes for sterilization by using 0.2% potassium permanganate disinfectant, taking the soaked cut slips out, placing the cut slips at a shady place for air drying, and tying the cut slips into bundles according to the standard of 10 or 30 pieces of cut slips after the surface moisture is completely dried, wherein care must be taken to ensure that the lower ends of the cut slips are neat; then, immersing the stems of which the base parts are not less than 3 centimeters of the whole bundle of cutting slips into the prepared rooting agent diluent in the direction of the cut at the lower end for quick dipping for 2 seconds; taking out the cutting and breeding on a disinfected cutting bed provided with a full-light automatic intermittent spraying device.
The rooting agent and the quick dipping method are used for treating the cutting slips of the paper mulberry to root, and after the cutting implementation application or test of not less than 250 ten thousand plants, 5 commercially available plant rooting hormones are synchronously used in the cutting slip rooting treatment process, and the results show that the rooting agent provided by the embodiment 11 of the invention has a more obvious effect and is superior to the 5 commercially available rooting hormones. The rooting effect graph of the paper mulberry cutting shoot using the rooting agent provided by the embodiment 11 of the invention is shown in the attached figure 4, the rooting survival rate averagely reaches 98.5%, adventitious roots begin to occur in 20 th to 25 th days after the paper mulberry cutting shoot is subjected to cutting, the vegetative roots of the cutting shoot reach the length of 1.9 to 2.9 centimeters in 36 th day, the number of root hairs reaches 25 to 35, and the paper mulberry cutting shoot callus and the skin hole can be induced to root together.
It should be noted that the specific dipping time in the above embodiment can be selected according to the difficulty of rooting of the tree species and the difference between the hard and tender branches; in addition, when the hair root agent diluent and the hair root agent base liquid are prepared and used, containers other than metal must be used.
In conclusion, the rooting agent provided by the embodiment of the invention is applied to the cutting seedling raising of various plants, cambium cells can be effectively activated, so that the cut forms callus in a short time, the decay of the cut is effectively controlled, a large amount of adventitious root primordium is formed inside the shoot stalk and the medullary ray cambium of the cut in a short time, the callus and the cortical adventitious root appear synchronously, the rooting time is obviously shortened, and the rooting is rapid. In addition, the rooting agent provided by the embodiment of the invention is applied to the cutting seedling production practice of a large batch of difficultly rooted tree species such as camellia oleifera abel, photinia serrulata, golden camellia with concave vein, and megalia gigas, the rooting rate and the seedling survival rate are obviously improved, the average rooting rate reaches 95.6%, and the production practice at the same time also shows that under the induction action of the invention, the callus or adventitious root of the plant cutting shoot can be synchronously rooted together and directly on the callus and cortex (skin pores) at the upper part of the cut; under the same condition, various rooting agents sold in the market at present are used for treating the plant cutting, the rooting rate can only reach 3-25%, and two parts of the cutting stem and the callus are not rooted together; therefore, compared with the prior art, the rooting agent provided by the embodiment of the invention is more suitable for the cutting seedling raising propagation of various plants and the transplanting of large-scale plants.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (6)
1. A rooting agent for cutting seedling is characterized by comprising the following components in parts by weight: 30-79 parts of indole auxin, 35-75 parts of naphthoic auxin, 0.0003-0.0013 part of steroid auxin, 0.2-5.5 parts of nutrient auxin and 0.5-6.5 parts of thiram bactericide;
the indole auxin is indolebutyric acid; the naphthoic auxin is naphthylacetic acid; the steroid auxin is natural brassinolide; the nutrient auxin is amino-oligosaccharide.
2. The rooting agent for cutting seedling as claimed in claim 1, wherein the thiram bactericide is one or more of thiram, arsine, zinc thiram, ferrum thiram, nickel thiram, arsine, ammonium thiram and sodium thiram.
3. A method for preparing a rooting agent according to any one of claims 1 to 2, comprising the steps of:
weighing indole auxin, naphthoic acid auxin, steroid auxin, nutritional auxin and thiram bactericide according to the weight parts of the components for later use; the indole auxin is indolebutyric acid; the naphthoic auxin is naphthylacetic acid; the steroid auxin is natural brassinolide; the nutrient auxin is amino-oligosaccharin;
the indole auxin, the naphthoic auxin, the steroid auxin, the nutritional auxin and the thiram bactericide are crushed and mixed to obtain the rooting agent.
4. A hair-rooting agent obtained by the production method as claimed in claim 3.
5. Use of a rooting agent according to any one of claims 1 to 2 and 4 for inducing rooting of cuttings in plants.
6. Use according to claim 5, characterized in that it comprises the following steps:
mixing the rooting agent with an ethanol solution with the volume concentration of 50-95% to obtain a rooting agent base solution; the mass ratio of the rooting agent to the ethanol solution is 1 (40-60);
diluting the rooting agent base solution with water to obtain a rooting agent diluent; the mass ratio of the rooting agent base liquid to water is 1 (199-999);
and carrying out quick dipping treatment on the plant cutting shoot by using the rooting agent diluent.
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| CN115152441B (en) * | 2022-06-13 | 2023-12-08 | 贵州中医药大学 | Cutting propagation method of blumea balsamifera serving as seedling medicine |
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| US4297125A (en) * | 1979-06-27 | 1981-10-27 | The United States Of America As Represented By The Secretary Of Agriculture | Tree rooting using synthetic auxins |
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Denomination of invention: A Rooting Agent for Cutting Seedlings and Its Preparation Method and Application Effective date of registration: 20230602 Granted publication date: 20210713 Pledgee: Agricultural Bank of China Limited Yihuang County sub branch Pledgor: JIANGXI RUIBOTE BIOTECHNOLOGY CO.,LTD. Registration number: Y2023980042821 |
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Granted publication date: 20210713 Pledgee: Agricultural Bank of China Limited Yihuang County sub branch Pledgor: JIANGXI RUIBOTE BIOTECHNOLOGY CO.,LTD. Registration number: Y2023980042821 |