CN113455508B - Rooting agent for Wenchang cone cutting slips and use method thereof - Google Patents
Rooting agent for Wenchang cone cutting slips and use method thereof Download PDFInfo
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- CN113455508B CN113455508B CN202110553420.2A CN202110553420A CN113455508B CN 113455508 B CN113455508 B CN 113455508B CN 202110553420 A CN202110553420 A CN 202110553420A CN 113455508 B CN113455508 B CN 113455508B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
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- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A01N33/22—Nitro compounds containing oxygen or sulfur attached to the carbon skeleton containing the nitro group having at least one oxygen or sulfur atom and at least one nitro group directly attached to the same aromatic ring system
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
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- A—HUMAN NECESSITIES
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- A01N39/00—Biocides, pest repellants or attractants, or plant growth regulators containing aryloxy- or arylthio-aliphatic or cycloaliphatic compounds, containing the group or, e.g. phenoxyethylamine, phenylthio-acetonitrile, phenoxyacetone
- A01N39/02—Aryloxy-carboxylic acids; Derivatives thereof
- A01N39/04—Aryloxy-acetic acids; Derivatives thereof
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Abstract
Rooting agent for Wenchang cone cutting and use method thereof comprise the following steps: naphthalene acetic acid, indolebutyric acid, compound sodium nitrophenolate, 2,4-D and deionized water, wherein the content of naphthalene acetic acid is 500-2000 mg/L, indolebutyric acid is 500-2000 mg/L, compound sodium nitrophenolate is 0.5-3.5 mg/L, and the content of 2,4-D is 0.5-2.5 mg/L, and the sheared cutting root is immersed in a rooting agent for 20-40 s, then inserted into a light matrix and placed on a seedling bed for seedling cultivation; through orthogonal tests and data processing analysis, the rooting agent plays an important role in the process of forming roots of the Wenchang cone cutting shoots, the single use of the compound sodium nitrophenolate has obvious rooting effect on the cutting shoots, and the compound formula of the compound sodium nitrophenolate and the naphthylacetic acid can obviously improve the rooting effect of the cutting shoots. In addition, the compound sodium nitrophenolate has a larger influence on the rooting rate of the cutting shoots, and has a smaller influence on the area of the callus. Can realize the efficient cutting seedling of the Wenchang cone.
Description
Technical Field
The invention relates to the technical field of plant cutting, in particular to a rooting agent for Wenchang cone cutting and a use method thereof.
Background
The Wenchang cone (Castanopsis wenchangensis) is a plant of the genus Cone (Castanopsis) of the family Fagaceae, has a very small species group in Hainan, has high nut nutritive value, has long eating history in Hainan Wenchang and Hainan regions, is an excellent special dry fruit economic tree species resource, and has very local characteristics. The Wenchang cone has small distribution range and small population and individual quantity, and in recent years, wenchang cone resources and habitat are often interfered by typhoon and manually damaged, especially the manual damage is extremely serious, and the quantity of germplasm resources is drastically reduced; in addition, the nuts of the Wenchang cone are eaten by birds, mice or other animals, the offspring and seedlings are updated slowly, so that ecological unbalance is caused, and the protection and the seed expansion of the Wenchang cone population are urgent.
Compared with other asexual propagation methods such as grafting, tissue culture and the like, the rapid propagation of seedlings by the cutting (cutting) technology has the characteristics of high propagation speed, high quality, capability of effectively regulating and utilizing the stage development rule of trees and the like. The growth substances of different plants play different roles in cutting, and after different concentrations and different types of rooting agents are used for treating different types of cutting slips for different time, certain influence is generated on endogenous hormone synthesis and distribution, and finally, the difference of rooting effects of the cutting slips is caused. Up to now, no research has been done on the amphibian cutting.
The invention provides a rooting agent for Wenchang cone cutting slips and a use method thereof, and solves the technical problems.
Disclosure of Invention
A rooting agent for cutting Wenchang cone comprises naphthylacetic acid, indolebutyric acid, compound sodium nitrophenolate and deionized water.
Preferably, the rooting agent for the Wenchang cone cutting shoots further comprises 2,4-D (2, 4-dichlorophenoxyacetic acid).
Preferably, the content of the naphthalene acetic acid in the rooting agent for the Wenchang cone cutting shoots is 500-2000 mg/L, the content of the indolebutyric acid is 500-2000 mg/L and the content of the compound sodium nitrophenolate is 0.5-3.5 mg/L.
Preferably, the content of 2,4-D of the rooting agent for the Wenchang cone cutting shoots is 0.5-2.5 mg/L.
Preferably, the content of the naphthalene acetic acid is 900-1500 mg/L, the content of the indolebutyric acid is 900-1500 mg/L, the content of the compound sodium nitrophenolate is 2-3.5 mg/L, and the content of the 2,4-D is 0.8-1.5 mg/L.
Preferably, the content of the naphthalene acetic acid in the rooting agent for the Wenchang cone cutting shoots is 1000mg/L, the content of the indolebutyric acid is 1000mg/L, the content of the compound sodium nitrophenolate is 2.5mg/L, and the content of the 2,4-D is 1mg/L.
The application method of the rooting agent for the Wenchang cone cutting shoots comprises the following steps:
firstly, filling purchased light matrixes into a tree seedling raising tray for standby;
step two, cutting and sterilizing the cutting slips successively;
step three, immersing the sheared cutting base into a rooting agent for 20-40 s;
and step four, inserting the cutting slips into the sterilized light matrix, and placing the cutting slip seedling raising trays on a seedbed in an integrated manner.
Preferably, the cutting length for shearing in the second step is 3-5 cm and the cutting is one leaf and one bud.
Preferably, the method for using the rooting agent for the Wenchang cone cutting shoots comprises the step two of putting the cutting shoots cut in the step two into a 5% carbendazim solution for disinfection.
Preferably, in the method for using the rooting agent for the Wenchang cone cutting shoots, the cut cutting shoot basal part is immersed in the rooting agent for 20-40 s at a speed of 1-2 cm.
Preferably, in the method for using the rooting agent for the Wenchang cone cutting shoots, the depth of the cutting shoots inserted into the light matrix in the fourth step is 1 cm-2 cm, and each hole is one plant.
Preferably, the application method of the rooting agent for the Wenchang cone cutting shoots further comprises the step five of arranging a sunshade net on the seedbed, wherein the area of the sunshade net is 60% -80%.
Preferably, the method for using the rooting agent for the Wenchang cone cutting shoots carries out micro-spray irrigation on the cutting shoots in the step five at intervals of 25-35 min.
The advantages are as follows: the patent is focused on the rooting agent for the Wenchang cone cutting, the data such as the survival rate, the rooting rate, the callus generation rate and the like of the rooting agent corresponding to different contents are counted according to the analysis of the plant characteristics of the Wenchang cone and through multiple tests, and the rooting agent suitable for the Wenchang cone is obtained through data analysis. Is beneficial to the effective and rapid asexual reproduction of the Wenchang cone and solves the problems of small number and low survival rate of the current Wenchang cone.
Description of the drawings:
the embodiments are further described below with reference to the accompanying drawings, in which:
FIG. 1 is a graph showing the effect of different exogenous hormone proportioning treatments on survival rate and rooting rate of Wenchang cone;
FIG. 2 is the effect of different exogenous hormone ratios on the area and rate of callus generation of Wenchang cone;
FIG. 3 is the effect of different exogenous hormone proportioning treatments on the number of primary adventitious roots of Wenchang Cone tassel strips;
the invention will be further described in the following detailed description in conjunction with the above-described figures.
Detailed Description
1. Overview of the test site
The test is located in the nursery garden (20 degrees 0 '38.71' N, 110 degrees 21 '58.07' E) of tropical gardening institute of agricultural sciences, hainan province, the average annual temperature is 23.8 ℃, the maximum temperature is 28.1 ℃, the minimum temperature is 18.5 ℃, the annual average sunshine hours are over 2060h, the annual average precipitation is 1664mm, the annual average relative humidity is 84.0%, and the low latitude tropical northern border of the ground belongs to the tropical marine climate.
2. Instrument and reagent
FA2004B electronic balance (ten-thousandth), shanghai Ping Xuan scientific instruments limited; electronic digital vernier calipers for Mitutoyo, sanfeng, japan, yifeng electronic instruments, inc. of Wujiang; picus Nxt electronic single-channel pipettor, shanghai Sharp analytical instruments, inc. A (naphthalene acetic acid), B (indolebutyric acid), C (2, 4-D), D (compound sodium nitrophenolate) and professional rooting agent, which are purchased from Shanghai Guangrui Biotechnology Co., ltd; deionized water is self-made in the laboratory.
3. Test method
The test was performed in a water-saving micro-sprinkler environment (once every 30 min). Filling the purchased light matrix into a 30-hole tree seedling tray for standby. The length of the cutting head is 3-4 cm. Cutting the cutting slips, immediately putting the cutting slips into a 5% carbendazim solution for disinfection. Then dipping the base of the cutting into rooting agents with different treatments for 30s, wherein the length of the base of the cutting is about 1.5 cm. The treated cutting is inserted into the sterilized light matrix, 1 plant is planted in each hole, and the depth is about 1.5 cm. After the treatment is finished, the cutting seedling raising trays are placed on a seedling bed in an integrated way, and 75% of sunshade net is arranged on the seedling bed.
4. Test design
Test using L25 (5) 6 ) Orthogonal test protocol designs, but only four factors and five levels, are detailed in table 1. The four factors are respectively: a (naphthalene acetic acid), B (indolebutyric acid), C (2, 4-D), D (compound sodium nitrophenolate). There were 25 treatments (denoted A1-a25 respectively), a blank CK (treated with clear water), and 1 professional rooting agent control. Each treatment was repeated 3 times with 30 cuttings per repeat. The test time was continued for 60d.
Table 1L 25 (5 6 ) Orthogonal test plan design
Note that: the rooting agent in the treatment is a special rooting agent purchased in the market, and the rooting rate is more than 90%.
5. Measurement method
The test starts at 25 at 4/2020 and ends at 25/2020. After 60d cutting, the survival rate of cutting slips is counted, and the callus generation rate and the number of primary adventitious roots are counted by 10 randomly strains. Based on this statistics, the calculation is performed: (1) Callus production rate = (number of long callus pieces/number of surviving shoots) ×100%; (2) Survival rate = (number of viable shoots/total number of shoots) ×100% (no rooting at this time is counted as cutting non-survival, 60 days); (3) Rooting rate= (rooting number/total number of branches) ×100%; (4) Callus area (S) =pi dh (d, callus diameter; h, callus length); the scoring criteria for different grades are set according to the statistics of different growth indexes, see (Table 2)
Table 2 scoring criteria for each index
6. Results and analysis
(1) Influence of different treatments on survival rate of Wenchang cone cutting shoots
As can be seen from FIG. 1, the survival rate of different treatments is 24.44% -86.57% after 60d cutting, and the survival rate is less than or equal to 40% and is 33.33%, 37.78%, 40.00%, 24.44% and 40.00% respectively by A10, A18, A23, A24 and A25; wherein, the survival rate of A24 is lowest, and the survival rate of A7 is highest, which reaches 86.57% (figure 1). Analysis of variance results showed (table 3) that the differences between different exogenous hormone proportioning treatments were not significant, p=0.056 (p > 0.05); further LSD multiplex comparisons found that control treatments showed very significant differences in performance with a10, a16, a24, rooting agent showed very significant differences in performance with a16, a24 (P < 0.01); the differences between A2 and A21, A22 are not significant, and the differences between A7 and A16 are only insignificant (P > 0.05).
(2) Influence of different treatments on rooting rate of Wenchang cone cutting shoots
The effect difference of different exogenous hormone ratio treatments on the rooting rate of the Wenchang cone cutting shoots reaches a very significant level (P < 0.01) (Table 3), the treatments with the rooting rate of more than or equal to 60 percent are only A7, A16, A19 and A20, the rooting rates are respectively 83.27 percent, 78.33 percent, 60.00 percent and 77.78 percent (shown in figure 1), and the rooting rates of other treatments are all below 60 percent. The rooting rate of the treatment control treatment is 0, the rooting rate of the A24 treatment is only 6.67%, the rooting rate is extremely low, and the rooting agent (professional) has the same value as the rooting rate of the treatment A1 and is lower than 50%.
(3) Influence of different treatments on the callus area of Wenchang cone cutting slips
After 60 days of cuttage, as shown in FIG. 2, the callus produced area of different exogenous hormone ratios is 9.61-84.22 mm 2 Between them, the maximum value is A7 treatment, 84.22mm 2 The callus area value obtained by ck treatment is minimum and is only 9.61mm 2 The method comprises the steps of carrying out a first treatment on the surface of the The maximum and minimum differ by a factor of 8.58, wherein the area of callus generation of only A7, A11, A14 and A16 is 70mm 2 The above values are respectively: 84.22mm 2 、72.15mm 2 、 73.15mm 2 And 78.37mm 2 . Analysis of variance results shows that different exogenous hormones are matchedThe area difference generated from the treated calli was not significant (p=0.104, > 0.05); multiple comparisons of LSD found that the area of calli generated by the fresh water treatment was significantly different from A8, a11, a14, a20, A8 and A6 (p<0.05 A) is provided; while none of the other treatments were significant (table 3).
(4) Influence of different treatments on the callus generation rate of Wenchang cone cutting slips
The effect of different treatments on the callus generation rate of the Wenchang cone cutting slips is great, and FIG. 2 shows that the callus generation rate of the A7 treatment reaches 75.66 percent, and the callus generation rate of the A16 treatment reaches 77.78 percent; whereas calli treated with A10, A18 and A24 had a yield of 33.33%, 37.78% and 24.44%, respectively, the highest and lowest differences were about 3.30 times; analysis of variance results showed (see table 3) that the differences between different exogenous hormone proportioning treatments were not significant (p=0.067, > 0.05); further LSD multiplex comparisons found that ck was very different from a10, a16, a24, the (professional) rooting agent was very different from A2, a21, a22 and a24 (P < 0.01), and none of the other treatments was significant.
TABLE 3 analysis of variance for different treatments
(5) Influence of different treatments on the number of first-stage adventitious roots of Wenchang cone cutting shoots
The different exogenous hormone proportioning treatments were very significant in the number of primary adventitious roots of the Wenchang cone cuttings (P=0.001, <0.01, table 3). ck can not generate adventitious roots, the A18, A23 and A24 processes generate 1.67, 1.54 and 1.00 of primary adventitious roots respectively, and the other processes are all over 2; wherein, the number of primary adventitious roots treated by the rooting agent (special), A1, A3, A7, A8, A11, A14, A15, A16, A19 and A20 reaches more than 7, and the number of primary adventitious roots treated by the rooting agent (special) is 12.21 (figure 3). The LSD multiple comparison shows that the (professional) rooting agent is not significantly different from A11, A14 and A17, A5 and A9, A7 and A16 (p is more than 0.05), and the difference between other treatments reaches extremely significant level.
(6) Comprehensive evaluation of growth conditions of Wenchang cone cutting shoots by different treatments
The cutting is processed by different exogenous hormone proportions, the degree of difference shown by each index is different, the comprehensive judgment is difficult to be directly carried out on each index, and in order to carry out comprehensive evaluation on the performance of the cutting in different treatments, the score judgment is carried out on the indexes by referring to each treatment scoring standard. Statistical results (see table 4) show that the CK has a combined score of only 13.67, the combined score for different formulations is between 20.11 and 48.33, and the score of a24 is the smallest and is only 20.11; the total score of both treatments A7 and A16 was greater than 40 points, with the treatment A7 value being 48.33 points at maximum, 3.54 and 2.40 times CK (13.67 points) and A24 (20.11 points), respectively.
As shown in figures 1 and 2, the survival rate, the callus generation rate and the rooting rate of the treated A7 and A16 are all over 75 percent; the number of the first-stage fixed roots of A7 and A16 is also more, and the number of the first-stage fixed roots is respectively 12.21 and 11.00. Therefore, both treatments A7 and A16 can be used for cutting seedling, but the rooting rate of A7 is 83.27% as the highest.
TABLE 4 comprehensive evaluation scores for different treatments
| Processing number | Score value | Processing number | Score value | Processing number | Score value | Processing number | Score value |
| ck | 13.67 | A6 | 27.22 | A13 | 23.00 | A20 | 38.34 |
| Rooting agent | 27.07 | A7 | 48.33 | A14 | 37.00 | A21 | 32.99 |
| A1 | 35.00 | A8 | 31.00 | A15 | 35.02 | A22 | 32.63 |
| A2 | 33.22 | A9 | 30.67 | A16 | 43.64 | A23 | 24.33 |
| A3 | 25.00 | A10 | 23.34 | A17 | 32.67 | A24 | 20.11 |
| A4 | 31.00 | A11 | 39.56 | A18 | 22.04 | A25 | 26.00 |
| A5 | 27.05 | A12 | 28.99 | A19 | 39.00 |
(7) Influence of different growth hormones on Wenchang cone cutting index
To determine the effect of individual exogenous hormones on the Wenchang cone cutting index, the results of the orthogonal experiments (scores of each treatment) were analyzed in a very poor manner. The extremely poor (R) value reflects the effect of each influence factor on the growth index, with the larger the R value, the more pronounced the effect.
As shown in Table 5, the four exogenous hormones have similar influence on the survival rate, callus generation rate and comprehensive index of Wenchang cone cutting shoots, and R value sequences are that indolebutyric acid > sodium nitrophenolate > naphthalene acetic acid >2,4-D, namely B > D > A > C; in the comprehensive index, the R value of indolebutyric acid is 9.92 the maximum, the influence on cutting shoots is the maximum, and the R value is far greater than the R values of other three hormones; from the viewpoint of survival rate, the R value of the B hormone is 2.40, the influence on survival rate is the greatest, and the next is the D hormone, which is 2.00. For the callus production rate, the influence of B hormone on the callus production rate is the largest, the R value is 3.99, the influence of C on the callus production rate is smaller, and the R value is 1.11.
The R values are ordered as follows when the number of the four exogenous hormones generated on the first-class adventitious roots of the Wenchang cone cutting shoots is as follows: indoleacetic acid > naphthylacetic acid >2,4-D > complex sodium nitrophenolate, namely B > A > C > D, and the influence of B hormone on the number of adventitious roots is the greatest, the R value is 3.55, the influence of D hormone is the least, and the R value is 1.34. From the rooting rate, the R values are ordered as: complex sodium nitrophenolate > naphthylacetic acid = indolebutyric acid = 2,4-D, i.e., D > a = B = C; the maximum R value of the D hormone is 2.80, the effect results of the other three hormones on the rooting rate of the cutting shoots are consistent, and the R values are 2.00, so that the effect of the D hormone on rooting green is great. In the callus generation area, the influence of C hormone is the largest, the R value is 3.60, the A hormone is the next smallest, the D hormone is the smallest, the R value is only 0.80, and the influence is the smallest in all indexes, so that the influence is small and can be ignored in production.
TABLE 5 treatment margin R values for different hormones
7. Conclusion and discussion
Hormone concentration and variety are important factors influencing cutting rooting, and rooting capacity can be improved by adopting exogenous hormone to treat cutting slips. The former studies on plant cutting techniques have been conducted mostly by using a hormone alone, or only Naphthalene Acetic Acid (NAA), or only ABT rooting powder, or only IBA rooting powder, and the like, and the fact that only about 70% rooting rate can be obtained by using a single hormone in some experiments has been fully confirmed. If the thin-stalk A Ding Feng is soaked for 2 hours by 300 mg.L-1 NAA, the rooting rate is 78%; the cutting shoots are treated by 3000 mg.L-1 IBA, the rooting rate is 57.5%, and the 60S tender branch cutting shoots are soaked by 93.2 mmole K-IBA solution, and the rooting rate is 72.2%.
The research results of the former show that the mixed use of different exogenous hormones can lead the cutting to obtain higher rooting rate than the single use, but the Wenchang cone cutting test has not been researched and reported in the past, the test adopts the method of processing different exogenous hormones in proportion, the rooting rate can reach 83.27% for the first time, and the number of first-stage adventitious roots reaches 12.21. According to the comparison analysis, the research purpose of the plants easy to root can be achieved, and the high survival rate can be obtained by adopting a single exogenous hormone, and the survival rate is more than 85%; such as: the cutting shoots are treated by the geranium (Pvlurgonium hortorum) with 2000 mg.L-1 IBA, the rooting rate reaches 97.79%, the tender branch cutting shoots are treated by the Ginkgo biloba solution with 10umolIBA, the rooting rate reaches 88.89%, but for the Wenchang cone in the study, the rooting rate is 46.67% and 0 respectively by adopting a market professional rooting agent and a clear water contrast CK, and the rooting rate is about doubled by using 2,4-D and sodium nitrophenolate for the first time in the study and mixing with the naphthalene acetic acid and the indolebutyric acid in proportion, and the rooting rate is up to 83.27%, which is probably the reason that the rooting rate can be obtained by mixing in a high proportion, which is about 1.78 times of the formula of the market professional rooting agent.
Different exogenous hormone treatments have major and minor differences in the influence of different indexes in the formation process of adventitious roots of plant cuttings. In order to further clarify the influence of different indexes on cutting rooting in the forming process of the first-stage adventitious roots of the Wenchang cone cutting shoots by naphthalene acetic acid, indolebutyric acid, 2,4-D and compound sodium nitrophenolate, the orthogonal test results are subjected to extremely poor analysis. The analysis result shows that the four growth hormones to be tested have the same influence on the comprehensive index, survival rate and callus generation rate of the cutting slips, namely, the indolebutyric acid is more sodium nitrophenolate is more sodium naphthylacetate is more 2,4-D, the indolebutyric acid has the greatest influence on the two indexes, and the influence is less 2,4-D; the indolebutyric acid has larger influence on the primary adventitious root number, is similar to the survival rate and the callus generation rate, but the effect of the naphthylacetic acid on the primary root number is not small, and the sodium nitrophenolate is the compound sodium with the smallest influence after the second order. Unlike the traditional concept in the prior art, naphthalene acetic acid plays a leading role in the cutting process of Wenchang cone (indolebutyric acid plays a leading role in the traditional concept).
In conclusion, indolebutyric acid, naphthylacetic acid, compound sodium nitrophenolate and 2,4-D play an important role in the process of forming roots of the Wenchang cone cutting shoots, and especially the effect of the compound sodium nitrophenolate which is frequently ignored is obvious. Most of plant cuttage tests reported at present only apply naphthalene acetic acid and indolebutyric acid, and 2,4-D and sodium nitrophenolate are rarely applied. The result shows that the single use of the compound sodium nitrophenolate has obvious rooting effect on the cuttings, and the compound formula of the compound sodium nitrophenolate and the naphthylacetic acid can obviously improve the rooting effect of the cuttings. In addition, the compound sodium nitrophenolate has a larger influence on the rooting rate of the cutting shoots, and has a smaller influence on the area of the callus.
The rooting agent disclosed by the invention can realize efficient cutting seedling of the Wenchang cone, reduce the seedling cost of the Wenchang cone, promote the wide application of the Wenchang cone cutting seedling in forestation, and prove that the ideal cutting effect can be obtained by using 2,4-D and compound sodium nitrophenolate in a mixed mode.
The foregoing examples illustrate only a few embodiments of the invention and are described in detail herein without thereby limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of protection of the present invention is to be determined by the appended claims.
Claims (5)
1. A rooting agent for Wenchang cone cutting spikes is characterized in that: consists of 1000mg/L of naphthalene acetic acid, 1000mg/L of indolebutyric acid, 2.5mg/L of compound sodium nitrophenolate and 1mg/L of 2,4-D;
the application method of the rooting agent for the Wenchang cone cutting shoots comprises the following steps:
firstly, filling purchased light matrixes into a tree seedling raising tray for standby;
step two, cutting and sterilizing the cutting slips successively;
step three, immersing the sheared cutting base into a rooting agent for 20-40 s;
and step four, inserting the cutting slips into the sterilized light matrix, and placing the cutting slip seedling raising trays on a seedbed in an integrated manner.
2. The rooting agent for Wenchang cone cutting shoots according to claim 1, which is characterized in that: the length of the cutting slips used for shearing in the second step is 3-5 cm, and the cutting slips are one leaf and one bud; and placing the sheared cutting into a 5% carbendazim solution for disinfection.
3. The rooting agent for Wenchang cone cutting shoots according to claim 1, which is characterized in that: and thirdly, immersing the sheared cutting base 1 cm-2 cm into a rooting agent for 20 s-40 s.
4. The rooting agent for Wenchang cone cutting shoots according to claim 1, which is characterized in that: and step four, inserting the cutting slips into the light matrix to a depth of 1 cm-2 cm, wherein each hole is one plant.
5. The rooting agent for Wenchang cone cutting shoots according to claim 1, which is characterized in that: the method further comprises the step five of arranging a sunshade net on the seedbed, wherein the sunshade area of the sunshade net is 60% -80%; and step five, cutting shoots are subjected to micro-spray irrigation every 25 to 35 minutes.
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| CN103416434A (en) * | 2013-07-12 | 2013-12-04 | 江苏农景生态建设有限公司 | Plant rooting agent and preparation method thereof |
| CN103478132B (en) * | 2013-09-23 | 2015-09-09 | 甘肃省农业科学院经济作物与啤酒原料研究所 | A kind of Radix Angelicae Sinensis root-naked transplanting of nursing young plants in hothouses is urged root and is delayed seedling agent and its preparation method and application |
| CN105660741A (en) * | 2014-11-22 | 2016-06-15 | 天津佳瑞兴科技发展有限公司 | Growth regulator for xylophyta |
| CN106818484A (en) * | 2017-02-14 | 2017-06-13 | 唐春艳 | A kind of method for improving castanopsis concinna rooting rate |
| CN108781812A (en) * | 2018-06-22 | 2018-11-13 | 海南省农业科学院热带园艺研究所 | A kind of oil tea quickly rich root seedling cuttage and seedling culture method, seedling medium, root-growing agent |
| CN111296451B (en) * | 2020-04-07 | 2021-07-13 | 江西瑞博特生物科技有限公司 | Rooting agent for cutting seedling as well as preparation method and application thereof |
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