CN116711548A - Cutting propagation method for dulcamara twig - Google Patents
Cutting propagation method for dulcamara twig Download PDFInfo
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Abstract
The invention discloses a method for cutting and breeding a long-handle dulcamara twig, which is characterized by comprising the following steps of: selecting the upper section part of the current annual semi-lignified branch of the long-handled dulcimum and the twigs with different ages of 10-25 days as cutting slips and trimming, and S2: cutting in a nursery greenhouse for 7 months, and S3: pure loess is selected as a matrix of the current annual semi-lignified branch, and turfy soil is selected as the matrix: perlite = 3:1 as a matrix for shoots of different ages; selecting a substrate container, and S4: selecting a disinfectant, namely selecting a rooting agent prepared by mixing IBA and NAA of which the concentration is 100-300 mg/L, and S5: immersing the treated spike base into a growth agent container, and S6: cutting the treated cutting slips, and S7: curing after cutting, S8: recording the growth condition of the cutting slips after cutting. The invention determines the matrix combination, the rooting agent concentration and the optimal cutting selection so as to realize the optimal cutting propagation method of the duying twig.
Description
Technical Field
The invention belongs to the technical field of plant cultivation, and particularly relates to a cutting propagation method for a twig of Duying longifolia.
Background
The long-stem dulcimum is perennial arbor, is mainly distributed in the areas of two guangdong, yunnan and Hainan in China, starts to be firm in 8 years, has high economic value and medicinal value, the wood can be used for manufacturing furniture, farm tools and the like, the bark can be used as dye, the fruit can be eaten, the root can remove blood stasis and detumescence, and the leaf extract has the effects of resisting oxidation, resisting aging, whitening, diminishing inflammation, inhibiting cancers and the like, and is an important water source protection tree species.
In a natural state, long-handle dulcamara seeds have hard shells due to seed coats, the germination rate is low, and the sources of the seeds are deficient, so that the population quantity is small, and the resources are deficient. Therefore, with the continuous excavation of the value of Duying on the long stem, it is urgent to increase the survival rate of tree species to obtain more seedlings.
As one of asexual propagation, cutting can maintain excellent target characters, compared with a grafting propagation mode, the method does not need to consider tedious screening and cultivation of stocks, and is not limited by seasons and propagation places, so that the method is an ideal asexual propagation way of duying.
Therefore, a method for cutting and breeding the twig of Duying longicorn is provided.
Disclosure of Invention
In order to solve the technical problems, the invention designs a cutting propagation method for the long-shank dulcamara twig, which is characterized in that different parts of the current semi-lignified branch and the shoots of different ages are selected, cutting experiments are carried out by adopting rooting agents with different concentrations and matrixes with different types, and the optimal cutting selection, matrix combination and rooting agent concentration are determined, so that the optimal cutting propagation effect of the long-shank dulcamara twig is realized.
In order to achieve the technical effects, the invention is realized by the following technical scheme: the cutting propagation method of the dulcamara twig is characterized by comprising the following steps of:
s1: selecting the upper section of the current annual semi-lignified branch of the long-handled dulcimum and the twigs with different days of 10-25 days as cutting slips, and trimming;
s2: selecting 7 months to carry out cuttage in a nursery greenhouse;
s3: pure loess is selected as a matrix of the current annual semi-lignified branch, and turfy soil is selected as the matrix: perlite = 3:1 as a matrix for shoots of different ages; selecting a substrate container;
s4: a rooting agent is selected; the rooting agent is prepared by mixing IBA and NAA with the concentration of 100mg/L equivalent; the rooting agent is prepared by mixing IBA and NAA of 100 mg/L-300 mg/L in equal quantity;
s5: immersing the treated cutting base into a growth agent container;
s6: cutting the treated cutting slips;
s7: maintaining the cutting slips after cutting;
s8: recording the growth condition of the cutting slips after cutting.
Further, in step S1, a healthy, pest-free long-handled Duying half-lignified branch with 2-3 lateral buds is selected.
Further, in the step S1, the flatness of the cut is ensured during cutting, the position of the cut at the lower end of morphology is about 0.5 cm minus or plus 0.1 cm away from the base internode, the inclination angle is 40 minus or plus 5 degrees, the cut at the upper end of morphology is 1 cm minus or plus 0.1 cm away from the top internode and is flatly cut, 1-2 leaves are reserved at the upper end of the cutting, the leaves are cut out by 1/2 size, and the length of each cutting is 8 cm minus or plus 0.5 cm to 15 cm minus or plus 0.5 cm; for tender branches, the tender branches must be broken off from the base parts of the branches, the original tissues of the base parts of the branches are reserved, 1-2 blades are reserved, the blades are cut off by 1/2 size, and the cut root parts of the cutting shoots are immersed into clear water for standby.
Further, in the step S4, disinfectant such as carbendazim, potassium permanganate and the like is selected to carry out basin soaking treatment on soil, and the soil is fully soaked in water and placed for 2 days for standby; the rooting agent used by the current annual semi-lignified upper-section branches of the long-handled dulcamara is prepared by mixing IBA and NAA with the same amount, and the concentration is 100mg/L; the rooting agent used by the shoots of 25 days old of Duying longpetiolus is prepared by mixing IBA and NAA with equal amount, and the concentration is 100mg/L.
In step S5, the base part of the cutting shoot which is collected and processed is immersed in a rooting agent container which is prepared by mixing IBA and NAA in equal quantity, and the rooting agent container stands for 28 to 30 minutes.
Further, the cutting in the step S6 comprises the following steps:
s6.1: before cutting, watering the cutting matrix to ensure that the cutting matrix is moist;
s6.2: selecting the upper, middle and lower three sections of cuttings of the current annual semi-lignified branches from pure loess and pure loess: sand = 1: 1. pure loess: cutting in a sandy soil=2:1 matrix, and selecting tender branch cuttings of different ages in 10-25 days to be in pure loess, pure sandy soil and turfy soil: cutting in perlite=3:1 medium;
s6.3: cutting the treated cutting slips into the matrix respectively, inserting the lower end of the cutting slips into 2-3 cm of the cutting slips in the matrix, punching holes in the matrix by using wood rods slightly thicker than the cutting slips in the cutting process to help cutting, compacting the matrix by hands after cutting, cutting 15-20 cutting slips per pot of matrix, and watering the cutting slips thoroughly after cutting, and carrying out film coating treatment.
Further, the method is characterized in that the maintenance after cutting in the step S7 comprises the following steps:
s7.1: placing the cutting slips subjected to cutting in a shady place;
s7.2: and (3) regularly checking and cleaning dead leaves and fallen leaves in the matrix, preventing the basal parts of the cutting slips from being spoiled, reducing watering after the cutting slips root, and spraying the leaves by using a watering can during watering.
Further, recording the growth condition of the cutting slips after cutting in the step S8 comprises the following steps:
s8.1: after the cutting slips grow in various environments and matrixes for a period of time, recording the growth conditions under various conditions, including growth speed, survival rate of the cutting slips, growth conditions of new roots and the like;
s8.2: analyzing the recorded data, and comparing growth conditions under different conditions, including the influences of different semi-lignified branch parts, different day-old shoots, different matrix combinations and different rooting agent concentrations on the growth of cuttings;
the beneficial effects of the invention are as follows:
1. different parts of the current annual semi-lignified branch and twigs of different ages are selected and trimmed, so that the growth vigor and survival probability of the cutting slips can be increased;
2. by selecting the combination of rooting agents with different concentrations and different types of matrixes, an environment more favorable for the growth of cutting slips can be created.
3. Through a scientific cutting method and environmental control, the growth of cutting slips can be further promoted, the success rate of cutting is improved, and the optimal cutting propagation effect of the duying twigs is realized.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed for the description of the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a flow chart of the steps of the cutting propagation method of the invention;
fig. 2 is a flowchart of a cutting step of the present invention;
FIG. 3 is a flow chart of the step of curing after cutting of the cutting slips of the invention;
FIG. 4 is a flow chart of the steps of the cutting propagation method for comparing and screening the optimal Duchesnea longifolia;
fig. 5 is a view of the rooting condition of the cutting in the present invention.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1:
referring to fig. 1 to 5, a duying cutting propagation method is characterized by comprising the following steps:
s1: selecting the upper section of the current annual semi-lignified branch of the long-handled dulcimum and the twigs with different days of 10-25 days as cutting slips, and trimming; selecting a long-handle dulcimum-shaped current semi-lignified branch which is robust and free of plant diseases and insect pests and has 2-3 side buds, ensuring the smoothness of a cut when cutting the cutting slips, enabling the position of the cut at the lower end of morphology to be at a position which is about 0.5 cm+/-0.1 cm away from a basal section, enabling the inclination angle to be 40+/-5 degrees, enabling the cut at the upper end of morphology to be 1 cm+/-0.1 cm away from a top section, reserving 1-2 blades at the upper end of the cutting slips, shearing the blades by 1/2, enabling the length of each cutting slip to be 8+/-0.5 cm to 15+/-0.5 cm, reserving the original tissue of the basal section of the cutting slips when cutting the tender slips, reserving 1-2 blades, shearing the blades by 1/2, and immersing the root of the cut cutting slips into clear water for standby.
S2: and selecting 7 months to cut in a nursery greenhouse.
S3: pure loess is selected as a matrix of the current annual semi-lignified branch, and turfy soil is selected as the matrix: perlite = 3:1 as a matrix for shoots of different ages; a substrate container is selected.
S4: selecting a disinfectant; the rooting agent is prepared by mixing IBA and NAA of 100 mg/L-300 mg/L in equal quantity; soaking soil in disinfectant such as carbendazim, potassium permanganate, etc., and standing for 2 days; the rooting agent used by the current annual semi-lignified upper-section branches of the long-handled dulcamara is prepared by mixing IBA and NAA with the same amount, and the concentration is 100mg/L; the rooting agent used by the shoots of 25 days old of Duying longpetiolus is prepared by mixing IBA and NAA with equal amount, and the concentration is 100mg/L.
S5: immersing the treated cutting base into a growth agent container; immersing the base part of the cutting shoot which is collected and processed into a rooting agent container which is prepared by mixing IBA and NAA in equal quantity, and standing for 28-30 min.
S6: cutting the treated cutting slips; the cutting slip comprises the following steps:
s6.1: before cutting, watering the cutting matrix to ensure that the cutting matrix is moist;
s6.2: selecting the upper, middle and lower three sections of cuttings of the current annual semi-lignified branches from pure loess and pure loess: sand = 1: 1. pure loess: cutting in a matrix with sandy soil=2:1, and selecting shoots with different ages of 10-25 days to be in pure loess, pure sandy soil and turfy soil: cutting in perlite=3:1 medium;
s6.3: cutting the treated cutting slips into the matrix respectively, punching holes in the matrix by using wood rods which are slightly thicker than the cutting slips in the cutting slips 2-3 cm cutting process, compacting the matrix by hands after cutting, cutting 15-20 cutting slips by using each pot of matrix, and watering the cutting slips thoroughly after cutting and coating the cutting slips.
S7: maintaining the cutting slips after cutting; the maintenance after cutting of the cutting slips comprises the following steps:
s7.1: placing the cutting wood subjected to cutting in a shady place, keeping the humidity in a greenhouse between 80% and 90%, and controlling the temperature between 25 ℃ and 28 ℃;
s7.2: and (3) regularly checking and cleaning dead leaves and fallen leaves in the matrix, preventing the basal parts of the cutting slips from being spoiled, reducing watering after the cutting slips root, and spraying the leaves by using a watering can during watering.
S8: recording the growth condition of the cutting slips after cutting; recording the growth condition of the cutting slips after cutting, comprising the following steps:
s8.1: after the cutting slips grow in various environments and matrixes for a period of time, recording the growth conditions under various conditions, including growth speed, survival rate of the cutting slips, growth conditions of new roots and the like;
s8.2: analyzing the recorded data, and comparing growth conditions under different conditions, including the influences of different semi-lignified branch parts, different day-old shoots, different matrix combinations and different rooting agent concentrations on the growth of cuttings;
example 2:
cutting the current annual semi-lignified branch into an upper section, a middle section and a lower section, and cutting the three sections of different positions into pure loess and pure loess: sandy soil=1:1, pure loess: cutting in sandy soil=2:1, wherein the rooting agent concentration is 100mg/L, 200mg/L and 300mg/L under the condition that IBA and NAA are mixed in equal amount, and L9 (3 4 ) The uniform horizontal orthogonal test is carried out for 9 experimental treatments, and the specific design is shown in table 1; 3 replicates were set for each treatment, 20 plants were cut per replicate, and shoots of different ages were planted in pure loess, pure sandy soil, peatmoss: cutting is carried out in a perlite=3:1 matrix, the concentration and treatment mode of the rooting agent are the same as those of the lignified branches, and the specific design is shown in table 2.
TABLE 1 design scheme for orthogonal cutting experiments of current annual semi-lignified shoots of Duying
TABLE 2 design scheme for orthogonal cutting experiments of different days old shoots of Duying
The data processing method comprises the following steps: and comprehensively analyzing the collected test data by adopting Microsoft Excel and Spss data processing software.
Survival rate (%) =number of surviving cutting shoots ≡total number of cutting shoots x 100%.
Rooting rate (%) =rooting cutting number ≡cutting total number×100%.
Callus formation rate (%) =number of calli generated ≡total number of cuttings x 100%.
Average root number (bar) =total number of roots/total number of rooting cuttings.
Average root length (cm) =root length sum ∈root number sum.
Rooting index: rooting rate x average rooting number x average root length.
After 30 days of the experiment, the callus occurrence and rooting condition of the current annual semi-lignified branches of the long-handled dulcimum under different treatment conditions are observed, the results are shown in table 3, after observation once every week, the periodic observation shows that the callus of the current annual semi-lignified branch treatment 1 starts to form after 40 days of cutting, other treatments form the callus after 47 days at most, after 60 days of cutting, the adventitious roots of treatment 1, treatment 5 and treatment 9 are generated, the formation of the callus of the other treatment groups is less, the adventitious roots of the treatment 1 and treatment 5 are vigorous after 70 days, the adventitious roots of the treatment 1 and treatment 5 are mainly formed at the callus of the cutting, the adventitious roots of the treatment 9 are mainly formed at the skin part of the cutting, and the root of the cutting root of the other treatment groups is rotten, so that the death phenomenon occurs mostly. As can be seen from the comparison of experimental treatment results, under the condition of the same rooting agent concentration, the growth of the upper section of the semi-lignified branch in pure loess in the current year has remarkable advantages, the survival rate is remarkably higher than that of the other groups, and the growth speed is also higher; under the same substrate conditions, the survival rate gradually decreases with the increase of the rooting agent concentration. The cutting experiment observation of the whole current annual semi-lignified branch shows that the current annual semi-lignified branch is difficult to survive in a matrix containing sandy soil due to poor water retention performance of sandy soil, the temperature difference between the morning and evening in a culture area is large, the noon temperature is high, the lignified branch is caused to lose water rapidly, and the formation of callus is delayed, so that the current annual semi-lignified branch dies gradually in a new environment. The observation of the rooting condition of each group of cutting shoots in the pure loess shows that the rooting types of the cutting shoots are two types, namely root of the bark and root of the callus, the root of the callus is mainly used under the condition of a low-concentration rooting agent, and the root of the bark is mainly used under the condition of a high-concentration rooting agent. In the experimental process, the dead cutting slips which are withered are timely removed, and the other cutting slips which are polluted are prevented from being mildewed and spoiled.
After 15 days of the experiment, calli and rooting conditions of shoots of different days old of long-handled dulcis under different treatment conditions were observed, and the results are shown in Table 3. After that, the calli of the three groups c, d and h are observed once a week, and in the cutting processing groups with different ages, the calli of the three groups a, e and i are observed to be formed after 25 days after cutting, the calli of the three groups a, e and i are formed at the latest, and the calli of the three groups a, e and i are formed less after 32 days; the adventitious roots appear first in the treatment c 40 days after cutting, and the adventitious roots appear in the treatment d and the treatment h after two to three days gradually, and the adventitious roots appear in the treatment a and the treatment e at the latest, and the adventitious roots appear in the treatment d and the treatment h after cutting for 50 days. And after 55 days of cuttage, the adventitious roots grow vigorously after the treatment c of the adventitious roots. According to observation of a tender branch cutting experiment, the tender branch of 25 days old is easier to form larger callus, adventitious roots can grow out and grow quickly after cutting, under the condition of the same rooting agent concentration and cutting selection, the callus is difficult to grow out due to the fact that the ventilation performance of pure loess is poor, the callus cannot grow out under the condition of pure loess, the callus cannot grow out under the condition of pure sandy soil, and the time for callus to appear in a mixed matrix of turfy soil and perlite is short compared with the time for callus to grow out; in a mixed matrix of turfy soil and perlite: under the condition that the rooting agent concentration is 100mg/L, the survival rate and the rooting rate of the branches at 25 days old are the highest and are 96.7% and 33.3%; the rooting rate of the 20-day-old branches is 20% as low as 300mg/L rooting agent; the average survival rate of 10-day-old branches at 200mg/L is relatively low and is 91.7%, and the young branches have remarkable advantages in survival rate and callus formation time in comprehensive comparison, wherein the rooting effect on the cutting is large, the cutting is selected and the matrix is selected, and the rooting agent has the effect of determining whether roots grow out of the callus or the epidermis in the lignified branches.
TABLE 3 callus formation and rooting under different treatments
| Treatment of | Stage of callus formation | Start rooting period | Rooting vigorous period |
| 1 | 2022.8.24 | 2022.9.14 | 2022.9.24 |
| 2 | 2022.9.1 | 2022.9.20 | 2022.9.30 |
| 3 | 2022.8.27 | Without any means for | Without any means for |
| 4 | 2022.8.26 | Without any means for | Without any means for |
| 5 | 2022.8.24 | 2022.9.14 | 2022.9.24 |
| 6 | 2022.9.1 | 2022.9.20 | 2022.9.30 |
| 7 | 2022.9.1 | 2022.9.20 | 2022.9.30 |
| 8 | 2022.8.26 | Without any means for | Without any means for |
| 9 | 2022.8.25 | 2022.9.14 | 2022.9.24 |
| a | 2022.9.22 | Without any means for | Without any means for |
| b | 2022.9.18 | Without any means for | Without any means for |
| c | 2022.9.15 | 2022.9.30 | 2022.10.5 |
| d | 2022.9.17 | 2022.9.30 | 2022.10.5 |
| e | 2022.9.22 | 2022.10.11 | 2022.10.19 |
| f | 2022.9.18 | 2022.10.6 | 2022.10.11 |
| g | 2022.9.18 | 2022.10.7 | 2022.10.11 |
| h | 2022.9.15 | 2022.9.30 | 2022.10.6 |
| i | Without any means for | Without any means for | Without any means for |
TABLE 4 Effect of different treatments on cutting of Duying woodized shoots
As can be seen from table 4, at different treatment levels, the survival rates of the current perennial semi-lignified shoots were significantly different, with the survival rate of treatment 1 reaching 76.7% at the highest, treatment 5 reaching 61.7% at the next, treatment 9 reaching 55.0% all significantly higher than the other groups, and the survival rate of treatment 4 being only 3.3% at the lowest; the rooting rate has obvious difference under different treatments, wherein the rooting rate of the treatment 1 reaches 51.7% at the highest, the rooting rate of the treatment 9 reaches 35.0% at the next, the rooting rate of the treatment 5 reaches 33.3%, which is obviously higher than that of other groups, and the rooting rate of the treatment 3 and the rooting rate of the treatment 8 are 0.0% at the lowest and do not grow roots; there was no significant difference in the number of calli formed, with more roots per treatment 5; there was a significant difference in the longest root and shortest root, where the longest root under treatment 1 was 114.04mm, the shortest root was 2.04mm, the longest root under treatment 5 was 119.32mm, the shortest root was 3.34mm, the longest root under treatment 9 was 66.04mm, the shortest root was 3.08mm, and three groups of treatments 3, 4, 8 were not longest and shortest roots since they were not long, and there was a significant difference in root whisker count as a function of how many roots were grown per seedling of the long root plants, where the average root count of treatment 6 was 7 at most, treatments 3 and 8 were not long, and were the average root whisker count. When the annual semi-lignified branches are subjected to cuttage, under the treatment of different matrixes, rooting agent concentration and cuttage parts, the three groups of treatments 1, 5 and 9 are excellent, and the three treated matrixes are all pure loess, so that the pure loess matrix is more suitable for the cuttage of the annual semi-lignified branches, wherein the loess: the rooting rate survival rate of sandy soil=2:1 under the substrate condition is obviously reduced, the death rate of plants is higher, and the roots are more deteriorated, the water retention of the plants is not good due to poor water retention under the sandy soil condition, the survival rate, the rooting rate and the longest root and the shortest root are not obviously different in pure loess, the root number is obviously different in treatment 1 and treatment 9, the rooting agent concentration of treatment 9 can be presumed to be high, the elongation growth of the roots is more favorable, and the survival rate, the rooting rate and the root number are obviously different in the mixed substrate of loess and sandy soil due to different proportions, wherein the loess: sandy soil=1:1 is superior to loess: sand=2:1.
TABLE 5 Effect of different treatments on cutting of Duying shoots
As can be seen from table 5, the survival rates under the different treatments were significantly different, wherein treatments a, e, i were significantly different from the remaining 5 treatments, wherein treatment g was not significantly different from the remaining groups, the survival rates of treatments c and g were up to 96.7%, the survival rates of treatments b and d were next up to 91.7%, and the survival rate of treatment i was the lowest, 65.0%; the rooting rate under different treatments also has a significant difference, wherein the treatment c and the treatment d have a significant difference from the treatments f, g and h and a significant difference from the treatments a, b and e, wherein the rooting rate of the treatment c and the treatment d reaches 33.3% at the highest, the rooting rate of the treatment h reaches 20.0% at the next highest, and the rooting rate of the treatment a and the treatment e is 0.0% at the lowest; in the formation of calli, there is a significant difference in the effect of different treatments on the formation of calli from cuttings but not on the growth of roots, wherein the number of calli formed in treatments b and f is the greatest, 23 and 17 respectively, followed by treatments c and g, 12 and 11 respectively, and the number of calli formed in treatments a, e, i is the least, 3, 2 respectively, whereby it can be seen that in a pure sandy soil matrix calli are formed but not as much as the rest, since there is insufficient nutrient supply after calli formation resulting in no differentiation and no growth; there was a significant difference between the different treatments on the longest root and the shortest root, represented on the longest root, where the longest roots for treatments d and h were significantly higher than the other groups, the longest root for treatment d reached 179.4mm, followed by treatment h reached 127.8mm; the average root number of the tender branch cutting shoots under different treatments also has obvious influence, wherein the root number of the three groups of the treatment groups c, d and h is obviously higher than the root number of the treatment group f, the treatment group f is obviously higher than the treatment groups a, b and e, the average root number of the treatment group h reaches 4.5 roots at most, and the treatment groups c and d reach 4.1 roots, wherein the treatment group a and the treatment group e do not grow roots. In the tender branch cutting, the survival rate of the tender branch cutting slips is obviously different from that of pure loess under the treatment of two other substrates, wherein the peatmoss: perlite=3:1 and pure sandy soil have no obvious difference in survival rate, and the pure loess has strong viscosity and poor air permeability, and cannot effectively transport moisture, so that shoots cannot differentiate to form callus, and therefore cannot survive; rooting rate under different treatments, wherein the matrix is turfy soil: the rooting condition in the perlite=3:1 matrix is best, and the rooting rate of pure loess is lowest, because the turfy soil has better ventilation condition, so that the conditions of transporting nutrient substances and moisture are better, thereby promoting the growth of roots and the formation of callus; in the formation of the callus, the statistical data is the condition that the callus is formed but not grown, the data show that the quantity of the callus formed but not grown in the treatment b and the treatment f is significantly more than that in the treatment c and the treatment h, the callus formation number of the three groups of the treatment a, the treatment e and the treatment i is the lowest, the substrate conditions of the treatment b and the treatment f are pure sandy soil, and the substrate conditions of the treatment c and the treatment h are turfy soil: perlite=3:1, the substrate conditions for treatment a, e, i are pure loess, so that it can be presumed that pure loess is not suitable for cutting shoots, pure sandy soil cannot be redifferentiated so that roots cannot be formed, but the method has significant advantages for callus formation; on the rooting cutting slips, the root whisker number of each plant is also different, wherein the treatment a and the treatment e do not grow roots, the average root whisker number of the treatments c, d and h is obviously higher than that of the other groups, the corresponding matrixes are mixed matrixes of peatmoss and perlite, the average root whisker number of the treatment h is higher than that of the other two groups, the shoots of 20 days old are more suitable for growing under the high concentration of rooting agent, in conclusion, the shoot cutting is more suitable for cutting in the mixed matrixes of peatmoss and perlite, and the shoots of 10 days old are less suitable for cutting in the shoots of different days old, and the shoots of 10 days old are less than that of the other two treatments.
In the cutting cultivation of the current annual semi-lignified branch of the long-handled dulcimum, the cutting slips under the condition of the pure loess matrix have obvious differences in survival rate and rooting rate and in the formation of callus, the survival rate and rooting rate of the upper-section cutting slips with the rooting agent concentration of 100mg/L are superior to those of the other two groups in the three treatments of the pure loess, the survival rate of treatment 1 is up to 76.7%, the rooting rate is up to 51.7%, and 6 plants are treated on the formation of callus, so that the upper section of the current annual semi-lignified branch can better survive and root under the conditions of the pure loess matrix and the rooting agent concentration of 100mg/L.
In the cutting cultivation of branches of different ages of long-handled dulcimum twigs, the substrate condition is turfy soil: perlite=3:1, rooting agent concentration of 100mg/L, rooting rate of 25 days old cuttings, and better callus formation.
Claims (8)
1. The cutting propagation method of the dulcamara twig is characterized by comprising the following steps of:
s1: selecting the upper section of the current annual semi-lignified branch of the long-handled dulcimum and the twigs with different days of 10-25 days as cutting slips, and trimming;
s2: selecting 7 months to carry out cuttage in a nursery greenhouse;
s3: pure loess is selected as a matrix of the current annual semi-lignified branch, and turfy soil is selected as the matrix: perlite = 3:1 as a matrix for shoots of different ages; selecting a substrate container;
s4: a rooting agent is selected; the rooting agent is prepared by mixing IBA and NAA of 100 mg/L-300 mg/L in equal quantity;
s5: immersing the treated spike base into a growth agent container;
s6: cutting the treated cutting slips;
s7: maintaining the cutting slips after cutting;
s8: recording the growth condition of the cutting slips after cutting.
2. The method for cutting and breeding the twig of the dulcis thunbergii according to claim 1, wherein in the step S1, the current annual semi-lignified twig of the dulcis thunbergii which is strong, free of diseases and insect pests and has 2 to 3 lateral buds is selected.
3. The method for cutting and breeding the long-handle dulcimum branches according to claim 1, wherein in the step S1, flatness of cuts is ensured when cutting the cutting slips, the position of the cuts at the lower end of morphology is about 0.5 cm+/-0.1 cm away from base internodes, the inclination angle is 40+/-5 degrees, the cuts at the upper end of morphology are 1 cm+/-0.1 cm away from top internodes, 1-2 leaves are reserved at the upper end of the cutting slips, the leaves are cut off by 1/2 size, and the length of each cutting slip is 8+/-0.5 cm-15+/-0.5 cm; for tender branches, the tender branches must be broken off from the base parts of the branches, the original tissues of the base parts of the branches are reserved, 1-2 blades are reserved, the blades are cut off by 1/2 size, and the cut root parts of the cutting shoots are immersed into clear water for standby.
4. The method for cutting and breeding the dulcamara twig according to claim 1, wherein in the step S4, disinfectants such as carbendazim, potassium permanganate and the like are selected to carry out basin soaking treatment on soil, and the soil is fully soaked in water and placed for 2 days for standby; the rooting agent used by the current annual semi-lignified upper-section branches of the long-handled dulcamara is prepared by mixing IBA and NAA with the same amount, and the concentration is 100mg/L; the rooting agent used by the shoots of 25 days old of Duying longpetiolus is prepared by mixing IBA and NAA with equal amount, and the concentration is 100mg/L.
5. The method for cutting and breeding dulcamara twig according to claim 1, wherein in the step S5, the collected and processed base part of the spike is immersed in a rooting agent container which is prepared by mixing IBA and NAA in equal quantity, and is kept stand for 28-30 min.
6. The method for cutting propagation of dulcamara twig according to claim 1, wherein the cutting in step S6 comprises the steps of:
s6.1: before cutting, watering the cutting matrix to ensure that the cutting matrix is moist;
s6.2: selecting the upper, middle and lower three sections of cuttings of the current annual semi-lignified branches from pure loess and pure loess: sand = 1: 1. pure loess: cutting in a sandy soil=2:1 matrix, and selecting twigs of different ages of 10-25 days to obtain pure loess, pure sandy soil and turfy soil: cutting in perlite=3:1 medium;
s6.3: cutting the treated cutting slips into the matrix respectively, inserting the lower end of the cutting slips into 2-3 cm of the cutting slips in the matrix, punching holes in the matrix by using wood rods slightly thicker than the cutting slips in the cutting process to help cutting, compacting the matrix by hands after cutting, cutting 15-20 cutting slips per pot of matrix, and watering the cutting slips thoroughly after cutting, and carrying out film coating treatment.
7. The method for cutting propagation of dulcamara twig as set forth in claim 1, wherein the maintenance after cutting in step S7 comprises the steps of:
s7.1: and placing the cutting slips subjected to cutting in a shady place.
S7.2: and (3) watering regularly, checking and cleaning dead leaves and fallen leaves in the matrix regularly, preventing the basal parts of the cutting slips from being spoiled, and reducing watering after rooting of the cutting slips, wherein a watering can is used for spraying leaves during watering.
8. The method for cutting propagation of dulcamara twig according to claim 1, wherein the recording of the growth condition after cutting in step S8 comprises the steps of:
s8.1: after the cutting slips grow in various environments and matrixes for a period of time, recording the growth conditions under various conditions, including growth speed, survival rate of the cutting slips, growth conditions of new roots and the like;
s8.2: and analyzing the recorded data, and comparing the growth conditions under different conditions, including the influences of different semi-lignified branch parts, different day-old shoots, different matrix combinations and different rooting agent concentrations on the growth of cuttings.
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