CN113412737B - Efficient cutting propagation seedling raising method for celastrus angulatus - Google Patents
Efficient cutting propagation seedling raising method for celastrus angulatus Download PDFInfo
- Publication number
- CN113412737B CN113412737B CN202110804283.5A CN202110804283A CN113412737B CN 113412737 B CN113412737 B CN 113412737B CN 202110804283 A CN202110804283 A CN 202110804283A CN 113412737 B CN113412737 B CN 113412737B
- Authority
- CN
- China
- Prior art keywords
- cutting
- rooting
- seedling raising
- ppm
- celastrus angulatus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/25—Dry fruit hulls or husks, e.g. chaff or coir
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
Abstract
The invention belongs to the technical field of plant breeding, and particularly discloses a high-efficiency cutting propagation seedling raising method for celastrus angulatus, which comprises the following steps: firstly, preparing a substrate; secondly, selecting cutting slips; thirdly, rooting treatment; fourthly, cuttage and management; fifthly, rooting and transplanting; sixthly, strong seedling culture. The method is simple and convenient to operate and low in cost, well solves the problems that the cutting slips of the first crown vines are susceptible to infection and black rot, greatly improves the cutting rooting effect, and has the rooting rate as high as 86%; the growth period is relatively short, the propagation efficiency is high, a large number of high-quality container seedlings can be quickly obtained, and the vigorous demands of landscaping and ecological restoration markets are met.
Description
Technical Field
The invention relates to the technical field of plant breeding, in particular to a high-efficiency cutting propagation seedling raising method for celastrus angulatus.
Background
The first Guanteng Bauhina corembsa is named as deep-cracked Bauhinia, is perennial evergreen woody liana of Bauhina genus of Fabaceae Bauhinia, and is produced in places such as Guangdong, Hainan and hong Kong, the flowering phase is 4-6 months, and the fruit phase is 9-12 months. The shape of the stem of the crow's foot is unique, is similar to the shape of a sheep hoof and is exactly similar to the nail of the same genus (Bauhiana purpurea), but the ratio of the leaves is about half smaller, and the ratio of the flowers is more delicate and lovely. The raceme grows on the lateral branches, and the petals are white, have pink veins, are gorgeous and emit aromatic odor; the pod is long and round in belt shape, is hung on the top, is red and lovely, has excellent landscape value of flower, leaf and fruit viewing, and can be widely applied to pavilion corridors, fences, wall walls or urban vertical greening and rock slope restoration.
The first Guanteng is loved by light, warm and high-temperature humid climate, is barren-resistant, has strong adaptability, is generally scattered in valley sparse forests or hillside sunny places, and has less artificial cultivation and landscape application. Currently, limited research reports indicate that: the setting rate of the first crown vines is not high, the seeds are difficult to germinate, the germination accelerating treatment process of the seeds is complex, and the seedling period of the seedlings is long. The first crown vine grows fast, the lateral branches are more, a large number of clone seedlings can be obtained quickly by utilizing the cutting propagation, but the cutting rooting is difficult, the cutting shoot is easy to be infected and generates black rot, the rooting rate is not high, the rooting quality is not good, and the requirement of the asexual propagation of the first crown vine cannot be met by adopting the common cutting method.
Disclosure of Invention
The invention aims to provide an efficient cutting propagation seedling raising method of the celastrus angulatus which can efficiently take roots, is quick in seedling formation and low in production cost, and solves the problems that the celastrus angulatus is easy to black rot in the cuttage and low in rooting rate in the prior art.
In order to achieve the purpose, the invention adopts the following technical scheme:
an efficient cutting propagation seedling raising method for celastrus angulatus comprises the following steps:
firstly, preparing a substrate: preparing a rooting matrix, and placing the rooting matrix on a seedbed in a greenhouse; preparing a growth substrate, and placing the growth substrate on a nursery land outside a greenhouse;
II, selecting cutting slips: selecting 2-3-year-old first crown vine branches which are robust in growth and free of diseases and insect pests every 4-5 months, shearing 1-2-year-old lateral branches on the branches to serve as scion branches, shearing 1-2 cuttings according to the length of xylem of the scion branches, ensuring that each cutting has the length of 7-10 cm, and reserving 2-3 pairs of small leaves or 2-3 small short branches with leaves;
thirdly, rooting treatment: the upper part and the lower part of each cutting slip are cut evenly, and the base part of each cutting slip is soaked in a rooting agent solution;
fourthly, cuttage and management:
(1) punching a hole on a rooting substrate, inserting the well-treated cutting slips into the hole, and thoroughly watering and rooting by using 1000-1500 ppm of carbendazim aqueous solution; then, opening a timing spray device for spraying and moisturizing;
(2) watering 1000-1500 ppm of carbendazim aqueous solution every 7 days to prevent germ breeding and cutting infection, and regularly checking a seedbed;
fifthly, rooting and transplanting:
(1) when the cutting culture is carried out for 2-3 weeks, the phloem of the base part of the cutting shoot expands to produce a root primordium and differentiates to form an adventitious root; when the adventitious roots continue to extend, gradually reducing the spraying frequency until the humidity of the white sky air in the shed is reduced to 60%, ventilating, and hardening the seedlings for 1-2 weeks; therefore, the adventitious roots of the cutting slips can be distributed in the holes to form stable root balls, and the rooting rate is counted to be 86%.
(2) Taking the rooted plantlets out and transplanting the rooted plantlets into the growth substrate, and then, thoroughly watering the rooted plantlets by using 1000-1500 ppm of carbendazim aqueous solution to fix roots;
sixthly, strong seedling culture: in order to promote the vigorous growth of the cutting seedlings, after 2 weeks of transplanting and field planting, the operations of spraying fertilizer on the leaf surfaces and dressing fertilizer on the roots are alternately carried out until the end of the year, and then the strong cutting seedlings meeting the requirements of engineering seedlings can be cultivated.
In some specific technical schemes, the rooting matrix is formed by uniformly mixing vermiculite and carbonized rice hull particles according to the volume ratio of 4: 1; the growth matrix is formed by uniformly mixing garden soil, peat soil, farmyard organic fertilizer and plain river sand according to the volume ratio of 4:3:1:2 or formed by uniformly mixing garden soil, peat soil, farmyard organic fertilizer and carbonized rice hull particles according to the volume ratio of 4:3:1: 2.
In some specific technical schemes, in the matrix preparation step, the rooting matrix is split-packaged by a 32-hole cuttage deep hole disc and then placed on a seedbed in a plastic greenhouse; the growth medium is separately packaged by 1 gallon plastic flowerpots and then placed on the nursery ground outside the greenhouse.
In some specific technical schemes, the growth matrix is packaged and sterilized by being thoroughly poured by potassium permanganate 1000ppm solution.
In some specific technical schemes, in the rooting treatment step, the rooting agent solution is prepared from 300-400 ppm of naphthylacetic acid, 400-600 ppm of indolebutyric acid, 50ppm of vitamins and 2000-3000 ppm of carbendazim.
In some specific technical schemes, in the rooting treatment step, the trimmed cutting slips are bundled into small bundles, the base parts of the cutting slips are soaked in a rooting agent solution for 15-20 min, and then the cutting slips are taken out and aired in a shade place for 5-10 min.
In some specific technical schemes, in the cutting and management step, the depth of the holes punched on the rooting substrate is 1/3-1/2 of the length of the cutting slips.
In some specific technical solutions, in the step of cutting and managing, the operation of spraying and moisturizing is: the fog spraying interval in the daytime is controlled by closing the water fog formed on the cutting blades and opening the cutting blades when the water fog disappears, and the fog spraying is closed at night to ensure that the humidity of the air in the sky is 90 percent.
In some specific technical schemes, in the cuttage and management step, when the seedbed is regularly checked, black rot cuttings or fallen leaves exist, and the cuttings or the fallen leaves are timely cleaned.
In some specific technical schemes, in the step of strong seedling culture, the operation of spraying the fertilizer on the leaf surfaces is that the compound aqueous solution fertilizer with 2000-3000 ppm of urea and 250-500 ppm of ammonium molybdate is sprayed on the leaf surfaces for 1 time every 20 days, and the compound aqueous solution fertilizer is continuously sprayed for 4-6 times; the operation of the root topdressing is to topdress 3000-5000 ppm aqueous solution fertilizer for the roots for 1 time and continuously dress for 3-5 times.
Compared with the conventional cutting propagation in the prior art, the invention provides the high-efficiency cutting propagation seedling raising method for the celastrus angulatus, which has the following beneficial effects:
1. the invention adopts different substrate formulas to respectively carry out cuttage rooting culture and transplantation strong seedling growth. The screened rooting matrix is loose and breathable, has good water-retaining property and does not contain germs and the like, is favorable for the differentiation of the root primordium of the cutting slips and the growth of the root system, and particularly can adsorb exogenous pollutants in the matrix and harmful endogenous substances formed by the oxidation of cuts of the cutting slips by utilizing the physical adsorption and chemical adsorption characteristics of carbonized rice husk particles, thereby effectively reducing the infection and black rot conditions of the cutting slips. The mixed matrix material in the screened growth matrix for transplanting is wide in source, rich in organic matter content, convenient to ventilate and drain, low in cost and very suitable for large-scale container seedling culture of the celastrus angulatus.
2. According to the invention, 1-2 year-old lateral branches on the main branches of the first crown vines are selected as cutting slips, the first crown vines grow fast, the main branches grow long, the number of the lateral branches is large, sufficient nutrients and endogenous hormones are accumulated in the lateral branches due to flowering requirements, and the first crown vines have higher physiological activity and rooting potential than the main branches. Under the condition of less initial materials, the lateral branch cuttage also has the advantage of high propagation coefficient, a large amount of clone seedlings can be quickly obtained, the main branches of the stock plants can still grow normally, and more cuttings for cuttage can be continuously germinated.
3. The invention adopts a mode of lower horizontal shearing instead of the commonly adopted 45-degree oblique shearing when the cutting is processed, because the medulla of the first crown vine cutting is in a semitransparent cross shape, the medulla has large water content and is not full of wood, the cut is easy to be infected or harmful substances are generated due to the activity change of peroxidase, and the horizontal shearing can effectively reduce the cut area of the cutting, thereby reducing the occurrence of infection and black rot.
4. The rooting agent solution adopted by the invention consists of a compounded growth regulator and a sterilization protective agent, the screened combination of 'naphthylacetic acid 300-400 ppm + indolebutyric acid 400-600 ppm' can effectively induce the phloem of the cutting shoot to differentiate to form an adventitious root, the vitamins and carbendazim with proper concentration can obviously reduce the incision infection and black rot condition of the cutting shoot, the use of the rooting agent solution greatly improves the cutting rooting effect of the celastrus angulatus, and the rooting rate reaches 86%.
5. The invention adopts the plastic greenhouse for spray cuttage, can maintain the high-temperature and high-humidity environment in the greenhouse, and has important effects on the water balance and the root system development in the rooting process of the first crown vine cutting slips. The continuous sterilization protection of the carbendazim can effectively prevent the breeding of germs on the seedbed and the occurrence of the infection of the cutting slips.
6. The gradual dehumidification and ventilation hardening operation adopted by the invention can improve the root quality of the cutting slips and the environmental adaptability of the cutting seedlings, is convenient for transplanting the rooted plantlets, and has the transplanting survival rate of more than 98 percent.
7. According to the method, 1-2-year-old fine lateral branches are used as cutting slips, the obtained cutting seedlings are tender and slow in later-stage seedling formation, strong seedling cultivation is performed through appropriate types and concentrations of leaf fertilizers and root topdressing, efficient absorption of nutrient elements such as nitrogen, phosphorus and potassium required by growth and development of the cutting seedlings can be continuously supplemented, and particularly, the requirement of the first crown vines (leguminous plants) on molybdenum fertilizers can be effectively supplemented through leaf surface spraying of ammonium molybdate fertilizers, so that nitrogen fixation and growth and development of nodules are promoted.
In addition, the method is simple and convenient to operate and low in cost, the problems that the cutting slips of the celastrus sinensis are susceptible to infection and black rot are well solved, the cutting rooting effect is greatly improved, and the rooting rate is as high as 86%; the growth period is relatively short, the propagation efficiency is high, a large number of high-quality container seedlings can be obtained quickly, and the vigorous demands of landscaping and ecological restoration markets are met.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic diagram of a conventional main branch cutting method of celastrus orbiculatus;
FIG. 2 shows the growth of the cutting slips of celastrus angulatus after the cutting of the main branches is conventionally adopted;
FIG. 3 is a schematic view of the medulla of the main branch of Stephania cepharantha;
FIG. 4 shows the case of the main branch of Stephania coronaria after being cut;
FIG. 5 shows the breeding situation of the main branch of Stephania cepharantha after 45-degree oblique cutting;
FIG. 6 is a growth comparison of lateral branches as cuttings and main branches as cuttings;
FIG. 7 shows the growth of lateral branches as cuttings;
FIG. 8 is a comparison of rooting by 45 degree oblique and horizontal shears according to the present invention.
Detailed Description
The technical solutions of the present invention will be described clearly and completely below, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In order to better illustrate the technical advantages of the invention, the invention is described in further detail below with reference to different method examples and the accompanying drawings.
In the research and development process of the invention, 4 cuttage method tests are carried out in sequence, and each test method is implemented as follows:
the cuttage method comprises the following steps: conventional main branch cuttage
(1) Selecting strong 1-2 year-old first crown vine branches, cutting the main branches into a plurality of short cuttings, ensuring that each cutting has 2 nodes, and cutting off lateral branches on the nodes.
(2) Selecting garden soil and peat soil as a cutting matrix, uniformly mixing according to the volume ratio of 3:1, subpackaging by using nutrient cups, vertically inserting the pruned cutting slips into the matrix, and thoroughly watering and sterilizing by using a carbendazim 1000ppm solution.
(3) Setting up a small sunshelter, shading with a shading net with shading degree of 50%, periodically checking the dryness and wetness of the cutting slips and the pot soil, and spraying water in time for humidification. And performing conventional water and fertilizer maintenance management after the cutting rooting.
And (2) a second cuttage method: rooting agent and main branch cuttage
The method is consistent with the first cutting method in steps, and is different from the first cutting method in that: soaking the base part of the cutting shoot in 800ppm solutions of naphthylacetic acid and indolebutyric acid for 15 min; and (3) spraying and moisturizing the cuttings by adopting a timing spraying device, wherein the spraying time is 9-18 hours per day, and the spraying is carried out for 5min every 3 hours.
A third cuttage method: rooting agent and lateral branch cuttage
The method is basically consistent with the first cutting method in steps, and is different from the first cutting method in that:
(1) and (3) cutting lignified lateral branches growing for 1-2 years on the branches to serve as cutting slips, and ensuring that each cutting slip has 2-3 leaves or small short branches with leaves.
(2) The base of the cutting is soaked in 800ppm solutions of naphthylacetic acid and indolebutyric acid for 15 min.
(3) And (3) spraying and moisturizing the cuttings by adopting a timing spraying device, wherein the spraying time is 9: 00-18: 00 every day, and the cuttings are sprayed for 5min every 3 hours.
(4) And pouring a carbendazim solution of 1000ppm for sterilization every 8-10 days after cuttage.
The cuttage method comprises the following steps: the method of the invention
An efficient cutting propagation seedling raising method for celastrus angulatus comprises the following steps:
firstly, preparing a substrate:
(1) rooting substrate: vermiculite and carbonized rice hull particles are uniformly mixed according to the volume ratio of 4:1 to form a rooting matrix, and the rooting matrix is subpackaged by a 32-hole cuttage deep hole disc and placed on a seedbed in a plastic greenhouse.
(2) Growing a substrate: garden soil, peat soil, farmyard organic fertilizer and carbonized rice hull particles (which can be replaced by plain river sand) are uniformly mixed according to the volume ratio of 4:3:1:2 to form a growth matrix, the growth matrix is thoroughly poured and sterilized by potassium permanganate solution of 1000ppm, and then 1 gallon plastic flowerpots are subpackaged and placed on nursery lands outside a greenhouse.
II, selecting cutting slips: selecting 2-3-year-old first crown vine branches which are strong in growth and free of diseases and insect pests every 4-5 months, shearing 1-2-year-old lateral branches on the branches to serve as scion branches, shearing 1-2 cuttings according to the length of xylem of the scion branches, ensuring that each cutting has the length of 7-10 cm, and reserving 2-3 pairs of small leaves or 2-3 small short branches with leaves.
Thirdly, rooting treatment: and evenly shearing the upper part and the lower part of each cutting, bundling the trimmed cutting into small bundles, soaking the base part of the cutting in a rooting agent solution for 15-20 min, and then taking out and airing in a shade place for 5-10 min. Wherein the rooting agent solution is prepared from 300-400 ppm of naphthylacetic acid, 400-600 ppm of indolebutyric acid, 50ppm of vitamins and 2000-3000 ppm of carbendazim.
Fourthly, cuttage and management:
(1) firstly, punching a hole on a rooting substrate by using a small wood stick, wherein the depth is 1/3-1/2 of the length of a cutting slip, vertically inserting the processed cutting slip into the hole, and thoroughly watering and rooting by using 1000-1500 ppm aqueous solution of carbendazim; and then, opening a timing spray device in the greenhouse for spraying and moisturizing, so that a high-temperature and high-humidity environment in the greenhouse can be maintained, and the method plays an important role in water balance and root system development in the rooting process of the celastrus angulatus cutting. The fog spraying interval in the daytime is controlled by closing the water fog formed on the cutting blades and opening the water fog when the water fog disappears, the fog spraying is closed at night, and the humidity of the air in the greenhouse in the daytime is ensured to be about 90 percent.
(2) 1000-1500 ppm of carbendazim aqueous solution is poured into the tray once every 7 days, and the seedbed germ breeding and the cuttings infection can be effectively avoided through the continuous sterilization protection of the carbendazim. The seedbed is inspected regularly, and when black and rot cuttings or fallen leaves exist, the cuttings or the fallen leaves need to be cleaned in time.
Fifthly, rooting and transplanting:
(1) when the cutting culture is carried out for 2-3 weeks, the phloem of the base part of the cutting shoot expands to generate a milky root primordium and differentiates to form milky adventitious roots; when the adventitious roots continue to extend, gradually reducing the spraying frequency in the greenhouse, lifting the greenhouse for ventilation when the humidity of the white sky air in the greenhouse is reduced to 60%, hardening the seedlings for 1-2 weeks in such a way, enabling the adventitious roots of the cutting slips to be fully distributed in holes and form stable root clusters, and counting the rooting rate to 86%.
(2) Carefully taking the rooted plantlets out of the hole tray, transplanting the rooted plantlets into a plastic pot filled with the growth substrate, and then thoroughly watering the rooted plantlets with 1000-1500 ppm of carbendazim aqueous solution.
Sixthly, strong seedling culture: in order to promote vigorous growth of cutting seedlings, after 2 weeks of transplanting and field planting, spraying 2000-3000 ppm of urea and 250-500 ppm of ammonium molybdate compound aqueous solution fertilizer on leaf surfaces for 1 time every 20 days, and continuously spraying 4-6 times; meanwhile, 3000-5000 ppm of monopotassium phosphate aqueous solution fertilizer is applied to roots of the plants for 1 time every month, and the application is continuously carried out for 3-5 times. Alternately carrying out the operations of spraying fertilizer on the leaf surfaces and dressing fertilizer on the roots till the end of the year, and culturing to obtain the robust cutting seedlings meeting the requirements of the engineering seedlings.
And (3) counting and comparing the four cutting methods, wherein the growth conditions of the cutting seedlings refer to attached figures 1-8, and the specific rooting data conditions refer to the following table 1.
TABLE 1 rooting data for the four cuttage methods
Note: the average rooting time refers to the time required for the average root length to be more than or equal to 0.5 cm;
the rooting quality index is the comprehensive index for evaluating the high and low rooting quality, namely the rooting rate multiplied by 50% + the average number of roots multiplied by 25% + the average root length multiplied by 25%.
As shown in fig. 1 and fig. 2, the celastrus angulatus is difficult to root by cutting with main branches, and the cutting slips are easy to infect, black rot, wilting and the like.
As shown in figure 3, the medulla of the celastrus angulatus cutting shoot is in a semitransparent cross shape, the medulla has high water content and is not full of wood, and the incision is easy to be infected or generate harmful substances due to the activity change of peroxidase. As shown in fig. 4, after the main branches are cut flatly, the cutting slips do not turn black; as shown in fig. 5, after the main branches are obliquely cut at 45 degrees, the cutting slips turn black; therefore, the medulla of the main branch of the first coronary rattan is not full, and the black rot of the cutting shoot is easily caused by infection; the cut area of the cutting slips can be effectively reduced by adopting flat shearing, so that the occurrence of infection and black rot is reduced.
As shown in fig. 6, the two cuttings on the right side are made by using the main branches and are all blackened, while the cuttings on the left side are made by using the side branches, so that the blackening phenomenon does not occur, and as can be seen, the side branches are not easy to be black and rot, and the main branches are easy to be black and rot. As shown in FIG. 7, the lateral shoot forms callus easily and takes root, but the cut under the cutting shoot is still susceptible to infection and gradually becomes black rot.
As shown in FIG. 8, the 45 ° oblique shearing of the lower cut of the cutting shoot on the left side still causes infection and has a small number of roots, while the flat shearing of the lower cut of the cutting shoot on the right side has little tendency to black rot and has a large number of roots.
The statistics of the rooting data of four embodiments in table 1, which adopt different cutting methods respectively, show that: the main branches are adopted for conventional cuttage, the rooting time of the cutting slips is long, and the cutting slips are easy to black rot or wilting, so that the rooting rate is low, and the rooting quality is not good; the rooting rates of the main branch and the lateral branch in cuttage are obviously different, the lateral branch in cuttage is easy to generate callus, the rooting rate is higher, and the lateral branch can be promoted to take root by treating the indolebutyric acid with the same concentration than by treating the naphthylacetic acid.
Compared with the first three methods, the high-efficiency cutting propagation seedling raising method shortens the rooting time, obviously improves the rooting rate of the cutting and obviously improves the rooting quality. Through reasonable strong seedling cultivation, the young cuttage seedlings sprout quickly and grow rapidly, and the method is suitable for large-scale production and application.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (10)
1. An efficient cutting propagation seedling raising method for celastrus angulatus is characterized by comprising the following steps:
firstly, preparing a substrate: preparing a rooting matrix, and placing the rooting matrix on a seedbed in a greenhouse; preparing a growth substrate, and placing the growth substrate on a nursery land outside a greenhouse;
II, selecting cutting slips: selecting 2-3-year-old first crown vine branches which are robust in growth and free of diseases and insect pests every 4-5 months, shearing 1-2-year-old lateral branches on the branches to serve as scion branches, shearing 1-2 cuttings according to the length of xylem of the scion branches, ensuring that each cutting has a length of 7-10 cm, and reserving 2-3 small short branches with leaves;
thirdly, rooting treatment: the upper part and the lower part of each cutting slip are cut evenly, and the base part of each cutting slip is soaked in a rooting agent solution;
fourthly, cuttage and management:
(1) punching a hole on a rooting substrate, inserting the well-treated cutting slips into the hole, and thoroughly watering and rooting by using 1000-1500 ppm of carbendazim aqueous solution; then, opening a timing spraying device for spraying and moisturizing;
(2) watering 1000-1500 ppm carbendazim aqueous solution every 7 days, and periodically checking the seedbed;
fifthly, rooting and transplanting:
(1) when the cutting culture is carried out for 2-3 weeks, the phloem of the base part of the cutting shoot expands to produce a root primordium and differentiates to form an adventitious root; when the adventitious roots continue to extend, gradually reducing the spraying frequency until the humidity of the air in the white sky in the greenhouse is reduced to 60%, and exercising the seedlings for 1-2 weeks;
(2) taking the rooted plantlets out and transplanting the rooted plantlets into the growth substrate, and then, thoroughly watering the rooted plantlets by using 1000-1500 ppm of carbendazim aqueous solution to fix roots;
sixthly, strong seedling culture: after 2 weeks of transplantation and field planting, alternately carrying out leaf surface spray fertilizer application and root topdressing, and culturing to obtain the robust cutting seedlings meeting the requirements of engineering seedlings at the end of the current year.
2. The efficient cutting propagation seedling raising method for celastrus angulatus according to claim 1, characterized in that: the rooting matrix is formed by uniformly mixing vermiculite and carbonized rice hull particles according to the volume ratio of 4: 1; the growth matrix is formed by uniformly mixing garden soil, peat soil, farmyard organic fertilizer and plain river sand according to the volume ratio of 4:3:1:2 or formed by uniformly mixing garden soil, peat soil, farmyard organic fertilizer and carbonized rice hull particles according to the volume ratio of 4:3:1: 2.
3. The efficient cutting propagation seedling raising method for the celastrus angulatus according to claim 1, characterized by comprising the following steps of: in the matrix preparation step, the rooting matrix is split-packaged by a 32-hole cuttage deep-hole disc and then placed on a seedbed in a greenhouse; the growth medium is separately packaged by 1 gallon plastic flowerpots and then placed on nursery lands outside the greenhouse.
4. The efficient cutting propagation seedling raising method for the celastrus angulatus of the claim 3, which is characterized in that: the growth medium is packaged and sterilized thoroughly by potassium permanganate 1000ppm solution.
5. The efficient cutting propagation seedling raising method for the celastrus angulatus according to claim 1, characterized by comprising the following steps of: in the rooting treatment step, the rooting agent solution is prepared from 300-400 ppm of naphthylacetic acid, 400-600 ppm of indolebutyric acid, 50ppm of vitamin and 2000-3000 ppm of carbendazim.
6. The efficient cutting propagation seedling raising method for the celastrus angulatus according to claim 1, characterized by comprising the following steps of: in the rooting treatment step, the pruned cutting slips are bundled into small bundles, the base parts of the cutting slips are soaked in a rooting agent solution for 15-20 min, and then the cutting slips are taken out and aired in a shade place for 5-10 min.
7. The efficient cutting propagation seedling raising method for the celastrus angulatus according to claim 1, characterized by comprising the following steps of: in the cutting and management step, the depth of the holes punched on the rooting substrate is 1/3-1/2 of the length of the cutting slips.
8. The efficient cutting propagation seedling raising method for celastrus angulatus according to claim 1, characterized in that: in the cuttage and management step, the spray moisturizing operation is as follows: the spraying interval in the daytime is controlled by closing when water mist formed on the cutting blades disappears and opening when the water mist disappears, and the spraying is closed at night to ensure that the humidity of the white sky air in the shed is 90 percent.
9. The efficient cutting propagation seedling raising method for the celastrus angulatus according to claim 1, characterized by comprising the following steps of: in the cuttage and management step, when the seedbed is regularly checked, black rot cuttings or fallen leaves are cleaned in time.
10. The efficient cutting propagation seedling raising method for the celastrus angulatus according to claim 1, characterized by comprising the following steps of: in the step of strong seedling culture, spraying urea 2000-3000 ppm and ammonium molybdate 250-500 ppm compound aqueous solution fertilizer to the leaf surface every 20 days for 1 time, and continuously spraying for 4-6 times; the operation of the root topdressing is to topdress 3000-5000 ppm aqueous solution fertilizer for the roots for 1 time and continuously dress for 3-5 times.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110804283.5A CN113412737B (en) | 2021-07-16 | 2021-07-16 | Efficient cutting propagation seedling raising method for celastrus angulatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110804283.5A CN113412737B (en) | 2021-07-16 | 2021-07-16 | Efficient cutting propagation seedling raising method for celastrus angulatus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113412737A CN113412737A (en) | 2021-09-21 |
| CN113412737B true CN113412737B (en) | 2022-07-15 |
Family
ID=77721072
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110804283.5A Active CN113412737B (en) | 2021-07-16 | 2021-07-16 | Efficient cutting propagation seedling raising method for celastrus angulatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113412737B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113826513B (en) * | 2021-09-24 | 2023-02-03 | 广州华苑园林股份有限公司 | Asexual rapid propagation seedling raising method for celastrus orbiculatus |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101438661B (en) * | 2008-12-16 | 2010-10-27 | 中国科学院华南植物园 | Method for greening roof |
| TWI455681B (en) * | 2011-09-30 | 2014-10-11 | Zhan Wen Liao | Methods for rapidly growing a tree |
| CN102919023B (en) * | 2012-11-09 | 2014-03-26 | 中国科学院华南植物园 | Method for breeding bauhinia galpinii efficiently and rapidly |
| CN104604484B (en) * | 2015-01-16 | 2018-05-15 | 中国科学院华南植物园 | A kind of method of barren hill greening |
| CN106888948A (en) * | 2017-01-17 | 2017-06-27 | 深圳市铁汉生态环境股份有限公司 | A kind of seed propagation method of first hat rattan |
| CN111456046A (en) * | 2020-05-13 | 2020-07-28 | 岭南生态文旅股份有限公司 | Light ecological slope restoration structure and restoration method |
-
2021
- 2021-07-16 CN CN202110804283.5A patent/CN113412737B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN113412737A (en) | 2021-09-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101518189B (en) | Cuttage propagation method of Virginia oak | |
| CN102113445B (en) | Method for raising seedlings of Pinus kesiya var.langbianensis by efficient twig cuttage propagation | |
| Hernández et al. | Cultivation systems | |
| CN102349430B (en) | Method for breeding seedlings of grapes by using containers | |
| CN113826513A (en) | Asexual rapid propagation seedling raising method for celastrus orbiculatus | |
| CN111758529A (en) | Cuttage seedling raising method for taxus chinensis in greenhouse container and medium | |
| CN111264328A (en) | Cultivation method of small bougainvillea spectabilis pot culture | |
| CN113412737B (en) | Efficient cutting propagation seedling raising method for celastrus angulatus | |
| CN113951140A (en) | Method for promoting rapid propagation of seedlings of paris polyphylla young plants | |
| CN111685127B (en) | Inducer for promoting vegetative propagation of plants and application thereof | |
| CN112889504A (en) | Method for raising seedlings of black chokeberry by cutting in greenhouse with turfy soil plug | |
| CN104255382A (en) | Planting method for raising survival rate of greening trees by twice transplanting | |
| CN108207474B (en) | Cutting seedling raising method for compound-color crape myrtle | |
| CN107409896B (en) | Sowing and seedling raising method for golden panda trees | |
| CN110558130B (en) | Cutting method of cauliflower | |
| CN110024676B (en) | Rapid rooting and seedling raising method for water culture cutting of betula alnoides | |
| GB2609995A (en) | Cultivation method of seedling from seed of wild ancient tea tree and method for preparing seedling tea | |
| CN111887082A (en) | Bougainvillea spectabilis native cold-resistant variety breeding method and application | |
| Chowdhuri | Performance evaluation of different growth regulators on propagation of Chinese juniper (Juniperus chinensis Var. pyramidalis) in subtropical zone | |
| CN104584828A (en) | Cutting seedling method for choerospondias axillaris twigs | |
| CN105075602A (en) | Method for producing seed potatoes | |
| CN111183808A (en) | Cuttage propagation method for nitraria twigs | |
| CN113519331B (en) | Method for large-area large-scale planting of taxus chinensis | |
| CN109729859B (en) | Grafting method for dwarfing and annual flowering of cananga odorata | |
| CN117441592A (en) | Reproduction method of rare camellia plants |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20210921 Assignee: Guangdong Chuanglin Seedling Co.,Ltd. Assignor: HUAYUAN LANDSCAPE ARCHITECTURE Co.,Ltd. Contract record no.: X2023980035410 Denomination of invention: An Efficient Cutting Propagation and Seedling Raising Method for the First Crown Vine Granted publication date: 20220715 License type: Common License Record date: 20230510 |