CN113142058B - Cutting propagation method for viburnum sargentii - Google Patents
Cutting propagation method for viburnum sargentii Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/22—Improving land use; Improving water use or availability; Controlling erosion
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/40—Afforestation or reforestation
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Abstract
The invention provides a cutting propagation method of viburnum sargentii, which comprises the steps of sequentially carrying out incising treatment, disinfection treatment and rooting treatment on the base of a stem segment of the viburnum sargentii, then cutting the base of the stem segment in a cutting medium to obtain a cutting seedling after the viburnum sargentii roots, and transplanting the cutting seedling together with the cutting medium. The method is simple, efficient and rapid, the rooting rate of the viburnum sargenti is as high as 95%, the seedling rate is high, the technical problems that the viburnum sargenti is low in rooting rate and seedling rate and the viburnum sargenti is in short supply in the seedling market are solved, and the application of the viburnum sargenti in garden greening is wider.
Description
Technical Field
The invention relates to the field of plant propagation, and particularly relates to a method for cutting propagation of viburnum sargentii.
Background
Viburnum sargentii (Latin scientific name: viburnum macrocephalum fort.) is a Caprifoliaceae family. Viburnum belonging to deciduous leaf or semi-evergreen shrub has height of 4 m, grey brown or grey gray bark, paper leaf, small teeth at edge, net-connected lateral edges, and umbellate inflorescence, and is composed of large white flowers with diameter of 16 cm, and is beautiful and blossoms in 4-5 months. Viburnum sargentii is mainly used for greening tree species in cities and courtyards, and its ball flower flock is accumulated like snowballs, bunched in oval green leaves, and erects on both sides of streets and courtyards to form a beautiful landscape, so that its arched flower branches can form a flower gallery. If the plant is planted in front of a court and in front of a window below a wall, the plant is also suitable for being used as a central tree of a large flower bed, is a wild woody flower and a precious excellent landscaping and ornamental tree species, and has high economic and research values.
The seed setting rate and the survival rate of the viburnum sargenti are low, so that few research reports aiming at the reproduction of the viburnum sargenti are reported at present. Viburnum sargentii is generally bred by adopting a grafting method, but the cost of the grafting method is higher; in the prior art, the viburnum sargentii is bred by adopting a cutting technology, but the rooting rate of the existing cutting technology is low and is only 40% -50%, so that the seedling rate of the viburnum sargentii is low, the viburnum sargentii is relatively short in the seedling market, and the application of the viburnum sargentii in garden greening is limited to a great extent. Therefore, the problem of the viburnum sargentii germchit propagation with high rooting rate and high survival rate needs to be solved urgently.
In view of the above, the present invention is particularly proposed.
Disclosure of Invention
Based on the defects, the invention aims to provide a method for cutting propagation of viburnum sargentii, which is simple, efficient and rapid, and through the cutting propagation method, the rooting rate of viburnum sargentii is as high as 95%, the lowest rooting rate is more than 60%, the seedling rate is higher, the technical problems that the viburnum sargentii is low in rooting rate and seedling rate and the viburnum sargentii is in short supply in the seedling market are solved, and the application of the viburnum sargentii in garden greening is wider.
The invention is realized by the following steps:
a cutting propagation method of viburnum sargenti comprises the following steps: sequentially carrying out incising treatment, disinfection treatment and rooting treatment on the base of the stem segment of viburnum sargentii, then cutting the base of the stem segment in a cutting medium to obtain a cutting seedling after the viburnum sargentii roots, and transplanting the cutting seedling together with the cutting medium. The method is simple, efficient and rapid, and the rooting rate of the viburnum sargentii is as high as 95 percent, the lowest rooting rate is more than 60 percent, and the seedling rate is higher through the cutting propagation method.
Said scoring of said stem segment base comprises scoring 1-3, preferably 2, of said stem segment base; the invention promotes the respiration rate of plant cell tissue by incising the base of the stem segment, on one hand, the cells can be promoted to be converted into meristem, and on the other hand, the transportation of soluble compounds to the lower part of the branch can be enhanced, thereby accelerating the cell division, improving the rooting capacity of the cuttings and effectively promoting the rooting. However, when the nicking treatment is higher than 3, the surface of the base of the stem segment is damaged to a higher degree, so that the stem segment is easy to be infected with mould, the stem segment is rotted and died, and the rooting rate is greatly reduced;
preferably, the carving mode of the stem segment base is longitudinal carving, and the longitudinal carving is parallel to the direction of the stem segment. The nicking mode has a great influence on the rooting rate, the longitudinal nicking can ensure a good rooting rate, the transverse nicking can lead to a low rooting rate, and when a plurality of nicks are transversely nicked, even the nicks can be caused, and the rooting rate is zero.
The rooting promoter adopted in the rooting treatment is 2,4-D and potassium indolebutyrate. The 2,4-D and the potassium indolebutyrate are combined for use, so that the synergistic effect can be realized, and the rooting agent is favorable for robustly rooting, more roots, longer roots and higher rooting rate.
The rooting treatment comprises soaking in a 2,4-D solution and dipping in a potassium indolebutyrate solution. The 2,4-D and the potassium indolebutyrate are combined to be used as the rooting promoter for treatment, the two hormones are used in a combined mode, so that the hormone is higher in stability and synergized, the rooting effect is better, the root primordium is formed faster, the rooting period is obviously shortened from 40 days to 30 days, if the 2,4-D solution is used for soaking, the root primordium forms cluster callus due to more callus formed on the root, the induced root system is fewer and shorter, the callus is more serious, and the death rate during transplanting is higher; when only the indole potassium butyrate solution is used for treatment, the rooting rate is low, the rooting rate is only about 50%, and the problems of few roots, thin and weak roots, undeveloped lateral roots and the like exist.
The concentration of the 2,4-D solution is 199-201PPM, and the concentration of the indole potassium butyrate is 100PPM-300PPM; the soaking time in the 2,4-D solution is 5-8min;
preferably, the concentration of the 2,4-D solution is 200PPM, and the concentration of the indole potassium butyrate is 200PPM; the soaking time in the 2,4-D solution is 5min. Under the condition, the rooting rate is as high as 95.0%.
In one embodiment, the 2,4-D solution is at a concentration of 200PPM and the potassium indolebutyrate is at a concentration of 100PPM; the soaking time in the 2,4-D solution is 5min.
In one embodiment, the 2,4-D solution is at a concentration of 200PPM and the potassium indolebutyrate is at a concentration of 300PPM; the soaking time in the 2,4-D solution is 5min.
In one embodiment, the 2,4-D solution is at a concentration of 200PPM and the potassium indolebutyrate is at a concentration of 100PPM; the soaking time in the 2,4-D solution is 8min.
The cutting medium is a mixed cutting medium comprising fine sand, peat soil and vermiculite; the volume ratio of the fine sand to the peat soil to the vermiculite is 2 (1-2) to 1, preferably 2. In the mixed cutting matrix, fine sand has a good water permeable effect, peat soil has a good air permeable effect, vermiculite has a good water retention effect, and the fine sand, the peat soil and the vermiculite are used in a matched manner according to a certain proportion, so that each matrix can achieve the best characteristics, and the high rooting rate of viburnum sargentii is facilitated.
The disinfectant adopted in the disinfection treatment comprises any one of carbendazim, potassium permanganate and thiophanate methyl;
preferably, the disinfectant is carbendazim, and the soaking time is 7-9min, more preferably 8min;
preferably, the disinfectant is potassium permanganate, and the soaking time is 3-5min;
preferably, the disinfectant is thiophanate methyl, and the soaking time is 12-15min.
Preferably, the disinfection treatment comprises soaking in 50% carbendazim solution for 8min.
Preferably, the viburnum sargentii cutting propagation method comprises the following steps: 2 stems of stem segments are carved and cut, the stems are soaked in 50% carbendazim solution for 8min, the stems are soaked in 200PPM 2,4-D solution for 5min after being dried, then the stems are dipped in 200PPM potassium indolebutyrate solution, the bases of the stem segments are inserted into a mixed cutting matrix of fine sand, peat soil and vermiculite with the volume ratio of 2 (1-2): 1, water is sprayed regularly for moisturizing, the stem segments are cultured for 25-40 days, cutting seedlings are obtained after the viburnum sargentii roots, and the cutting seedlings and the cutting matrix are transplanted. By adopting the preferable technical scheme, the viburnum sargentii has the advantages of faster and better rooting, high rooting rate and high seedling rate.
The length of the stem section is 10-15cm, the cutting row spacing is 5-8cm when the stem section is cut in the mixed cutting medium, and the cutting density is 140-160 plants/m 2 。
And after the viburnum sargentii is subjected to cuttage rooting in the matrix, transplanting the cuttage seedlings and the cuttage matrix into a pot, shading to avoid strong light irradiation, spraying water every day for moisturizing, and fertilizing and maintaining for about 15 days.
The fertilizer adopts compound fertilizer water solution with the proportion of N-P-K15-15-15, and the fertilizer application mode is that 2000 times of water is prepared into solution and then is sprayed.
The invention has the following beneficial effects:
(1) The method is simple, efficient and rapid, the rooting rate of the viburnum sargentii is as high as 95%, the lowest rooting rate is more than 60%, the seedling rate is high, the technical problems that the viburnum sargentii is low in rooting rate and seedling rate and the viburnum sargentii is short in the seedling market are solved, and the application of the viburnum sargentii in garden greening is wider.
(2) The method comprises the steps of firstly adopting the base of the stem segment to cut and treat, promoting cells to be converted into meristem, and simultaneously strengthening the transportation of soluble compounds to the lower part of branches, so as to accelerate cell division, forming a foundation for high rooting rate, then soaking the base in a disinfectant solution for a proper time, and jointly using two hormones, namely 2,4-D and potassium indolebutyrate, so that the hormone has higher stability and synergy, the rooting effect is better, the root primordium is formed faster, and the rooting period is obviously shortened.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
Example 1
A cutting propagation method of viburnum sargentii comprises the following steps: 2 stems are cut at the base of the stem section in a longitudinal cutting mode, the stem section is soaked in 50% carbendazim solution for 8min, the stem section is treated in 200PPM 2,4-D solution for 5min after being dried, then the stem section is dipped in 200PPM potassium indolebutyrate solution quickly, the stem section is inserted into a mixed cutting matrix of fine sand, peat soil and vermiculite with the volume ratio of 2.
Example 2
A method for cutting and propagating viburnum sargentii comprises the following steps: 1 stem section base is incised in a longitudinal incised mode, the stem section base is soaked in potassium permanganate solution with the concentration of 0.3% for 5min, the stem section base is treated in 2,4-D solution of 200PPM for 8min after being dried, then the stem section base is dipped in 100PPM potassium indolebutyrate solution quickly, the stem section is inserted into mixed cutting medium of fine sand, peat soil and vermiculite with the volume ratio of 2.
Example 3
A method for cutting and propagating viburnum sargentii comprises the following steps: 3 stems are cut at the base of the stem segment in a longitudinal cutting mode, the stem segment is soaked in 50% carbendazim solution for 9min, the stem segment is treated in 200PPM 2,4-D solution for 6min after being dried, then the stem segment is dipped in 300PPM potassium indolebutyrate solution quickly, the stem segment is inserted into a mixed cutting medium of fine sand, peat soil and vermiculite according to the volume ratio of 2.
Example 4 Effect of base-cutting of Stem segments on rooting of Viburnum sargentii by cutting
Keeping two to three leaves on a stem section with the length of 15cm, longitudinally cutting the base part of the stem section in parallel with the stem section, wherein the length of the longitudinal cutting is about 2cm of the base part, respectively longitudinally cutting 200 stem sections in 50 batches, wherein 4 stem sections in each batch are longitudinally cut 1, 2, 3 and 4, taking 50 non-cutting stem sections as comparative examples of 50 batches of cutting stem sections, respectively carrying out the same treatment on 50 batches of non-cutting stem sections and the 50 batches of four cutting stem sections, and carrying out the treatment by adopting the treatment method after the base part of the stem section is cut in the example 1 to obtain the average root number, the average root length and the root percentage of the 50 batches of non-cutting stem sections and the 50 batches of four cutting stem sections, wherein the root results are shown in the table 1:
TABLE 1 table of the effect of stem base cutting on rooting
Wherein the average rooting number is the number of new roots born in one of 50 stem segments.
Therefore, the rooting rate of 1-3 incised wounds is higher than that of non-incised wounds, the average rooting number, the average root length and the rooting rate are highest when the number of incised wounds is 2, and the average rooting number, the average root length and the rooting rate are greatly reduced when the number of incised wounds is more than 3, mainly because the incised wounds are large in number, the damage degree of the surface of a base part is high, mildew is easily infected, and the rot and the death are caused. Therefore, 1-3 stems are cut at the base of the stem segment, the rooting of the plant can be effectively improved, and the rooting rate is obviously increased.
Example 5 Effect of disinfectant soak time on Viburnum viburnum Diels cuttage rooting
Soaking seven groups of cutting slips in a disinfectant for 5min, 6min, 7min, 8min, 9min, 10min and 11min respectively, and studying the influence of soaking time on rooting of viburnum sargentii, wherein each group contains 50 cutting slips, and the disinfectant is a carbendazim solution with the concentration of 50%. After soaking, the seven groups of cutting slips are all treated by the method of the disinfectant of the embodiment 1, wherein the slips are taken from the cutting slips nursery of cymbidium faberi ltd. The average root number and average root length of the seven groups of cutting slips and the rooting results of the seven groups of cutting slips are shown in table 2:
TABLE 2 table of the effect of carbendazim disinfectant soaking time on root
Therefore, the soaking time is within the range of 7min to 9min, the rooting rate is high, when the soaking time is less than 7min, the rooting rate is reduced, along with the lengthening of the soaking time, when the soaking time is more than 9min, all rooting indexes are reduced, and the longer the soaking time is, the better the rooting index is. Therefore, when the carbendazim disinfectant solution is soaked for 7-9min, the rooting of plants can be effectively improved, and the rooting rate is remarkably increased.
Example 6 effects of rooting Accelerator treatment on rooting of viburnum sargentii cuttage
The 12 groups of stem segments are respectively soaked in 2,4-D solution of 200PPM for 3min, 5min, 8min and 10min and then are respectively dipped in potassium indolebutyrate solution of 100PPM, 200PPM and 300PPM, wherein 50 stem segments in each group are subjected to cuttage by adopting the method after the treatment of the rooting promoter in the embodiment 1, and the rooting result of each group of stem segments is shown in the table 3:
TABLE 3 rooting Accelerator treatment to rooting Effect TABLE
Therefore, when the seeds are soaked in 2,4-D solution of 200PPM for 3min and then quickly dipped in potassium indolebutyrate solutions of 100PPM, 200PPM and 300PPM respectively, the rooting rate is low; soaking in 200PPM 2,4-D solution for 5min, and quickly dipping in 100PPM, 200PPM and 300PPM potassium indolebutyrate solutions respectively to obtain high rooting rate, wherein the rooting rate is highest and is as high as 95% when the solution is soaked in 200PPM 2,4-D solution for 5min and then dipped in 200PPM potassium indolebutyrate solution; firstly soaking in 200PPM 2,4-D solution for 8min, and then quickly dipping in 100PPM potassium indolebutyrate solution, wherein the rooting rate is 75%, and the rooting rate is reduced after the concentration of the potassium indolebutyrate solution is increased; the rooting agent is soaked in 2,4-D solution of 200PPM for 10min, and then quickly dipped in potassium indolebutyrate solutions of 100PPM, 200PPM and 300PPM respectively, so that the rooting rate is low.
Example 7 Effect of matrix on rooting of Viburnum sargentii cuttings
Mixing fine sand, peat soil and vermiculite according to 7 different proportions (volume ratios) to prepare a substrate for cuttage, respectively inserting 7 groups of viburnum sargentii ear strips into the mixed cuttage substrate with the 7 proportions, wherein each group contains 50 viburnum sargentii ear strips, regularly spraying water for moisturizing, and counting the rooting condition of the viburnum sargentii ear strips in the substrates with different proportions after culturing for 35 days, wherein the ear strips are taken from the ear picking gardens of the cymbidium sargentii group company Limited. The results are shown in Table 4:
TABLE 4 influence of substrate on root results Table
Therefore, when the volume ratio of the fine sand to the peat soil to the vermiculite is 2. Therefore, under the condition of the substrate, the rooting rate is higher, and the seedling rate is higher.
Example 8 transplantation and management of viburnum sargentii
In examples 1 to 7, the viburnum sargentii is planted in the matrix for rooting, the cutting seedlings and the cutting matrix are transplanted into a pot, the pot is shaded to avoid strong light irradiation, water is sprayed every day for moisture preservation, and fertilization maintenance is performed for about 15 days.
The fertilizer adopts compound fertilizer water solution with the proportion of N-P-K15-15-15, and the fertilizer application mode is that 2000 times of water is prepared into solution and then is sprayed.
The supporting management measures in examples 1 to 7 are as follows:
viburnum sargentii is excavated out from the matrix after the matrix is cut and rooted by using a small shovel, the root system is not damaged as much as possible, the matrix with the root is transplanted into a prepared pot, decomposed chicken manure is added into the soil commonly used in the transplanting pot, and after transplanting, the soil of the base is gently extruded, so that the firmness is ensured, the viburnum sargentii is placed in shady place, and once water spraying is carried out every day for moisturizing so as to avoid death due to lack. Sterilizing with a carbendazim solution every 7 days, wherein the carbendazim solution is a solution obtained by diluting 50% carbendazim by 1000 times, fertilizing management is performed every 15 days, the fertilizer used is a compound fertilizer aqueous solution with a proportion of N-P-K15-15-15, shading treatment is removed after curing for 15 days, full light culture is performed, fertilizing is performed for 30 days, the fertilizer aqueous solutions are all compound fertilizer aqueous solutions with a proportion of N-P-K15-15-15, and finally viburnum sargentii is cured into commercial seedlings for sale.
In conclusion, the method is simple, efficient and rapid, the rooting rate of the viburnum sargentii is as high as 95%, the lowest rooting rate is more than 75%, the seedling rate is high, the technical problems that the viburnum sargentii is low in rooting rate and seedling rate and the viburnum sargentii is short in the seedling market are solved, and the application of the viburnum sargentii in garden greening is wider.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (11)
1. A method for cutting and propagating viburnum sargentii is characterized by comprising the following steps: sequentially carrying out incising treatment, disinfection treatment and rooting treatment on the base of the stem segment of viburnum sargentii, then cutting the base of the stem segment in a cutting matrix to obtain a cutting seedling after the viburnum sargentii takes root, and transplanting the cutting seedling together with the cutting matrix;
said scoring of said stem segment base comprises scoring 1-3 of said stem segment base;
the rooting treatment comprises soaking in 2,4-D solution and dipping in indole potassium butyrate solution;
the concentration of the 2,4-D solution is 199-201PPM, and the concentration of the indole potassium butyrate is 100PPM-300PPM; the soaking time in the 2,4-D solution is 5-8min;
the cutting medium is a mixed cutting medium comprising fine sand, peat soil and vermiculite; the volume ratio of the fine sand to the peat soil to the vermiculite is 2 (1-2) to 1.
2. The method for cutting propagation of viburnum sargenti according to claim 1, wherein the cutting treatment of the base of the stem segment comprises cutting 2 pieces of the base of the stem segment;
the stem segment base is carved in a longitudinal carving mode, and the longitudinal carving is a carving parallel to the direction of the stem segment.
3. The method for cutting propagation of viburnum sargenti according to claim 1, wherein the concentration of the 2,4-D solution is 200PPM, and the concentration of the potassium indolebutyrate is 200PPM; the soaking time in the 2,4-D solution is 5min.
4. The cutting propagation method of viburnum sargenti according to claim 1, wherein the volume ratio of the fine sand to the peat soil to the vermiculite is 2.
5. The cutting propagation method of viburnum sargenti according to claim 1, wherein the disinfectant adopted in the disinfection treatment comprises any one of carbendazim, potassium permanganate and thiophanate methyl.
6. The cutting propagation method of viburnum sargenti according to claim 5, wherein the disinfectant is carbendazim, and the soaking time is 7-9min.
7. The cutting propagation method of viburnum sargenti according to claim 5, wherein the disinfectant is carbendazim, and the soaking time is 8min.
8. The cutting propagation method for viburnum sargenti according to claim 5, wherein the disinfectant is potassium permanganate, and the soaking time is 3-5min.
9. The cutting propagation method of viburnum sargenti according to claim 5, wherein the disinfectant is thiophanate methyl, and the soaking time is 12-15min.
10. The cutting propagation method of viburnum sargenti according to claim 5, wherein the disinfection treatment comprises soaking in 50% carbendazim solution for 8min.
11. The cutting propagation method of viburnum sargenti according to claim 1, wherein the steps comprise: 2 stems are cut at the base part of the stem segment, soaked in 50% carbendazim solution for 8min, soaked in 200PPM 2,4-D solution for 5min after drying, dipped in 200PPM potassium indolebutyrate solution, then the stem segment base part is inserted in mixed cutting medium of fine sand, peat soil and vermiculite with the volume ratio of 2 (1-2): 1, water is sprayed regularly for moisturizing, the stem segment base part is cultured for 25-40 days, cutting seedlings are obtained after the viburnum sargentii roots, and the cutting seedlings and the cutting medium are transplanted.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202110554514.1A CN113142058B (en) | 2021-05-20 | 2021-05-20 | Cutting propagation method for viburnum sargentii |
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