CN105145579A - Composite cutting rooting agent for caragana as well as preparation method and application of agent - Google Patents
Composite cutting rooting agent for caragana as well as preparation method and application of agent Download PDFInfo
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Abstract
The invention discloses a composite cutting rooting agent for caragana as well as a preparation method and application of the agent, and belongs to the field of plant growth regulators. The agent mainly comprises indole-3-butyric acid potassium, lentinan and a grape vine leaching solution. The method comprises the following steps: compounding indole-3-butyric acid potassium, lentinan and sodium dehydroacetate; mixing the Kyoho grape vine leaching solution with the compound according to a certain ratio; soaking caragana cuttings in the mixture. The agent has the advantages that indole-3-butyric acid potassium, lentinan and sodium dehydroacetate are compatible and have an extremely remarkable synergistic effect, so that the defect that a single component is utilized can be effectively overcome, and sprouted caragana plants are thick and strong, developed in root system, and higher in stress resistance.
Description
Technical field
The present invention relates to cuttage root-taking agent, particularly relate to a kind of compound cuttage root-taking agent being active ingredient with various plants growth regulator and grape branch leaching liquor of applicable caragana, the invention further relates to this compound cuttage root-taking agent promoting the application in caragana cuttage rooting, raising cuttage survival rate, raising biocidal property and enhancing resistance etc., belonging to plant growth regulator field.
Background technology
Pulse family Caragana (Caraganasinica (Buc'hoz) Rehd) is mainly distributed in Arid Area to the north of Chinese Yellow river valley, southwest and northwest are then centered by Qinghai-Tibet Platean, North China, the Northwest's revegetation, enhancement of environment, check winds and fix drifting sand, the high quality forage resource of water and soil conservation, there is very strong drought adaptation ability, and also effect is improved to herbaceous plant Population Niche, Functional diversities of soil microbial communities etc. under shrubbery.Under the environment that Desert Regions, the Inner Mongol is extremely arid, the sexual propagation of caragana plant lacks provenance, and vegetative propagation cuttage survival rate is low.At present, improving cuttings survival rate is the important means expanding caragana cultivation scope.
For solving the problem, except Routine Management measure, common method is mechanical treatment, yellow process, immersion process, heat and urge root process, drug treating.Employing drug treating cuttings, production technology is simple, easy to use, treating capacity large, and cuttings can not only be allowed fully to absorb various active ingredient, also has the effect that vernalization is early sent out.
CN101707958A discloses the cuttage breeding method of desert caragana, involved plant growth regulator is indolebutyric acid, heteroauxin, No. ABT1, root-inducing powder, No. ABT2, root-inducing powder, this cuttage agent has certain effect in raising caragana survival rate, but not obvious in the effect improving the resistance such as caragana salt resistance.CN103155865A discloses a kind of caragana quick clone training seedling-growing method, and involved plant growth regulator is salicylic acid, and it does not substantially have effect in raising caragana cuttings drought resistance etc.
In order to overcome the agent of existing caragana cuttage root-taking in the deficiency promoting existence in caragana root growth, raising Seedling Stage resistance etc., need a kind of caragana compound cuttage root-taking agent that can promote cuttage rooting, raising cuttage survival rate, improve biocidal property, increase resistance of development badly.
Summary of the invention
Technical problem to be solved by this invention overcomes the deficiencies in the prior art, provides a kind of caragana compound cuttage root-taking agent that can promote cuttage rooting, raising survival rate, improve biocidal property and enhancing resistance.
Another technical problem to be solved by this invention is to provide a kind of method preparing the agent of described caragana compound cuttage root-taking.
Technical problem to be solved by this invention is achieved through the following technical solutions:
The agent of a kind of caragana compound cuttage root-taking, is grouped into by following one-tenth: butyric acid potassium, lentinan, dehydro sodium acetate and water.
Preferably, by mass percentage, butyric acid potassium 6-12%, lentinan 0.05-0.1%, dehydro sodium acetate 0.2-0.4%, surplus are water;
Preferred, the consumption of each composition is: butyric acid potassium 9%, lentinan 0.075%, dehydro sodium acetate 0.3%.
Butyric acid potassium is mainly used in cuttage rooting, can induce the formation of root substance, promotes Cell Differentiation and division, is conducive to the differentiation of the generation of new root and fibrovascular system, promotes the formation of cuttings adventive root, but root of its induction is carefully dredged, bifurcated is many.
Lentinan is from the fruit body of mushroom, be separated the polysaccharide obtained, it is a kind of important human immunity conditioning agent, can be used as the ancillary drug for the treatment of tumor disease, agricultural production is mainly used to the virus disease of prevention and control crop, plant or cell can be induced to produce and accumulate resisting substance and pathogenesis-related proteins.Lentinan and environmentally compatible, and chemical pesticide that is efficient, environmental protection is coordinated to use the consumption that can reduce chemical pesticide, but be subject to the impact of environmental condition, effect is unstable, is used alone and has limitation.
Dehydro sodium acetate is a kind of crystalline powder of white, and be the food preservative that health ministry approval uses, have the bactericidal action of wide spectrum, antibacterial range extensively and not must affect by pH value, under acid, neutral, alkaline environment, all have good antibacterial effect.
In order to eliminate the deficiency using single plant growth regulator, butyric acid potassium and Nafusaku, astragalus polyose, lentinan, potassium sorbate, dehydro sodium acetate etc. are had hestening rooting by the present invention, antibacterial composition is compatible, expect that screening obtains one and effectively can not only improve survival rate, effectively can also urge the caragana compound cuttage root-taking agent that root urgees to strengthen, increase resistance.
The present invention is found by screening experiment, butyric acid potassium and lentinan, dehydro sodium acetate being carried out the composite caragana compound cuttage root-taking agent obtained arranges in pairs or groups after grape branch leaching liquor uses, and is improving survival rate, hestening rooting and in biocidal property, drought resistance, salt resistance etc., is all significantly being better than other compounded combination.Three's compatibility significantly enhances the resistance of caragana together, has extremely significant Synergistic effect.Butyric acid potassium to the harmful effect of root can by lentinan and dehydro sodium acetate alleviate, it is still remained valid to the growth-promoting effect of root bar.
Impact between plant growth regulator is very complicated, may there is the multiple impact such as synergy, antagonism, so that likely there is the situation that binary built is better than ternary built.In addition, from the viewpoint of cost and preparation processing, reduce medicament kind and also there is obvious advantage; Given this, the present invention designs binary built test further and does further inspection to the effect of ternary built.Result of the test is visible, and the Be very effective that butyric acid potassium, lentinan and dehydro sodium acetate carry out ternary built is better than other binary built scheme (difference reaches pole significance level); Composite score is visible, and the composite effect of butyric acid potassium, lentinan and dehydro sodium acetate all obviously will be better than other compound scheme, confirms and butyric acid potassium and lentinan and dehydro sodium acetate is carried out compositely have significant Synergistic effect.
To be made up of butyric acid potassium, lentinan and dehydro sodium acetate and to have on the basis of Synergistic determining the agent of caragana compound cuttage root-taking, the present invention finds further, the different amounts proportioning of butyric acid potassium, lentinan and dehydro sodium acetate, for the impact also highly significant soaking effect.The present invention is found by a large amount of screening experiments, and three kinds of compositions carry out compatibility according to following amount ranges and have good synergy:
By mass percentage, butyric acid potassium 6-12%, lentinan 0.05-0.1%, dehydro sodium acetate 0.2-0.4%, surplus are water;
Wherein, three kinds of compositions carry out compatibility according to following consumption and have best synergy:
Butyric acid potassium 9%, lentinan 0.075%, dehydro sodium acetate 0.3%, surplus are water.
Invention further provides a kind of method preparing the agent of described caragana compound cuttage root-taking, the method comprises: butyric acid potassium, lentinan and dehydro sodium acetate are added to the water, and stirring and dissolving, to obtain final product.
In order to reach better technique effect, described water is preferably distilled water.
The present invention still further provides the agent of described compound cuttage root-taking and is promoting the application in caragana cuttage rooting, raising cuttage survival rate, raising biocidal property, enhancing resistance etc., specifically comprise: by described compound cuttage root-taking agent dilute with water, obtain the caragana compound cuttage root-taking agent after diluting; Caragana compound cuttage root-taking agent after caragana cuttings being diluted is soaked;
Wherein, preferably, described dilution is by described compound cuttage root-taking agent dilute with water 10000-20000 doubly, is preferably 12500-17500 doubly, is more preferably 15000 times.
The present invention finds further, after compatible to butyric acid potassium, lentinan, dehydro sodium acetate and grape branch leaching liquor, can the raising caragana cuttage survival rate of highly significant, short root short strong, improve biocidal property, strengthen the anti-adversity such as drought resistance and salt resistance.
Grape is a kind of by the fruit of extensively cultivating, and by the end of the year 2010, Chinese Grape area under cultivation will reach 8,280,000 mu, and gross yield reaches 8,430,000 tons, and kyoto grape is one of main breed.Will inevitably produce a large amount of discarded branches in the production of a large amount of grape, pruning process, if do not process, not only contaminated environment, also causes the waste of resource.Grape branch contain protein 8.1%, soluble sugar 10.2%, also containing 10 multivitamin such as Va, Vb, Vc, Ve, more than the 10 kind of mineral element such as Cu, K, Mg, Mn, 17 seed amino acids and the materials such as polyphenol, flavonoids such as methionine, asparatate, glutamic acid, lysine.In view of grape branch is rich in multiple beneficial material, make grape branch leaching liquor after being collected by grape branch, for the treatment of caragana cuttings, can turn waste into wealth.Be used alone grape branch leaching liquor and the growth rate that immersion treatment can improve caragana is carried out to caragana, but the biocidal property of caragana can be reduced.The present invention finds, grape branch leaching liquor and butyric acid potassium, lentinan, dehydro sodium acetate is compatible, and growth promotion effect of grape branch leaching liquor can perform to maximum, its to the deficiency of caragana biocidal property by will to minimum.
In order to screen the optimum amount proportioning of butyric acid potassium, lentinan, dehydro sodium acetate and grape branch leaching liquor, the consumption proportion of the present invention to four kinds of constituents screens, and more different consumptions is for cuttage rooting, the impact improving the aspects such as survival rate, raising biocidal property, increase resistance; Result of the test shows, the good compound scheme of effect is: butyric acid potassium 6-12%+ lentinan 0.05-0.1%+ dehydro sodium acetate 0.2-0.4%, with grape branch leaching liquor (grape branch leaching liquor stoste dilutes 5 times) dilution 15000 times; This consumption proportion is adopted to promote will significantly be better than in the effect such as root growth and raising resistance other consumption proportion (difference reaches significance level), wherein the effect of " butyric acid potassium 9%+ lentinan 0.075%+ dehydro sodium acetate 0.3%; with grape branch leaching liquor (grape branch leaching liquor stoste dilutes 5 times) dilution 15000 times " is that in all compound schemes, effect is best, is significantly better than other consumption proportion (difference reaches pole significance level) in this compound scheme.
Therefore, present invention also offers another caragana compound cuttage root-taking agent, be made up of composition I and composition II; According to mass ratio meter, composition I: composition II=1:10000-20000; Preferably, composition I: composition II=1:12500-17500; Most preferred, composition I: composition II=1:15000.
Wherein, described composition I is made up of butyric acid potassium, lentinan, dehydro sodium acetate and water; By mass percentage, in composition I, the consumption of each composition is: butyric acid potassium 6-12%, lentinan 0.05-0.1%, dehydro sodium acetate 0.2-0.4%, surplus are water; Preferably, the consumption of each composition is: butyric acid potassium 9%, lentinan 0.075%, dehydro sodium acetate 0.3%, surplus are water;
Described composition II is grape branch leaching liquor.
The preparation of described grape branch leaching liquor comprises: grape branch flooding is obtained grape branch lixiviate stoste; By grape branch lixiviate stoste dilute with water, to obtain final product; Preferably, by grape branch leaching liquor stoste dilute with water 2-20 doubly, preferred, by grape branch leaching liquor stoste dilute with water 2-8 doubly, most preferred, by grape branch leaching liquor stoste dilute with water 5 times.
As a kind of reference scheme, described grape branch lixiviate stoste prepares in accordance with the following methods:
(1) collect the grape winter and cut bar, natural seasoning;
(2) be added to the water after being pulverized by the branch of natural seasoning lixiviate, to obtain final product.
In order to reach better technique effect, the invention provides a kind of preparation method of preferred grape branch leaching liquor stoste, comprising the following steps:
(1) the grape winter is cut bar naturally to dry, pulverize and sieve;
(2) be added to the water in grape branch powder, lixiviate 0.5-3h under the temperature condition of 15-25 DEG C, filter, get filtrate, to obtain final product;
Wherein, preferably, according to g:ml meter, grape branch powder: water=1:9; At 20 DEG C of water-bath lixiviate 1h.
Invention further provides a kind of method preparing this caragana compound cuttage root-taking agent, comprising: composition I and composition II are mixed, to obtain final product.
Present invention also offers this compound cuttage root-taking agent and promote the application in the cuttage rooting, raising cuttage survival rate, raising biocidal property, enhancing resistance etc. of caragana; Concrete, described application comprises: the described caragana compound cuttage root-taking agent of caragana cuttings soaked; Wherein, cuttings root 3-4cm is soaked with caragana taking root liquid after the dilution of grape branch leaching liquor; Described immersion preferably soaks 2h at 20-30 DEG C of temperature.
Embodiment
Further describe the present invention below in conjunction with specific embodiment, advantage and disadvantage of the present invention will be more clear along with description.It should be understood that described embodiment is only exemplary, any restriction is not formed to scope of the present invention.It will be understood by those skilled in the art that and can modify to the details of technical solution of the present invention and form or replace down without departing from the spirit and scope of the present invention, but these amendments or replacement all fall into protection scope of the present invention.
The preparation of Preparative Example 1 grape branch leaching liquor
(1) the grape winter is cut bar naturally to dry, pulverized 40 mesh sieves, and kept in Dark Place;
(2) take grape branch powder 1g, add water by the solid-liquid ratio of 1:8 (g:ml), at 20 DEG C of water-bath lixiviate 1h, filter while hot, make grape branch leaching liquor stoste;
(3) by grape branch leaching liquor stoste dilute with water 5 times, to obtain final product.
The preparation of Preparative Example 2 grape branch leaching liquor
(1) the grape winter is cut bar naturally to dry, pulverized 30 mesh sieves, and kept in Dark Place;
(2) take grape branch powder 1g, add water by the solid-liquid ratio of 1:9 (g:ml), at 25 DEG C of water-bath lixiviate 2h, filter while hot, make grape branch leaching liquor stoste;
(3) by grape branch leaching liquor stoste dilute with water 2 times, to obtain final product.
The preparation of Preparative Example 3 grape branch leaching liquor
(1) the grape winter is cut bar naturally to dry, pulverized 50 mesh sieves, and kept in Dark Place;
(2) take grape branch powder 1g, add water by the solid-liquid ratio of 1:10 (g:ml), at 30 DEG C of water-bath lixiviate 0.5h, filter while hot, make grape branch leaching liquor stoste;
(3) by grape branch leaching liquor stoste dilute with water 20 times, to obtain final product.
The preparation of Preparative Example 4 Grape Skin leaching liquor
(1) Grape Skin is dried naturally, pulverize and sieve, keep in Dark Place;
(2) take Grape Skin powder 1g, add water by the solid-liquid ratio of 1:8 (g:ml), at 20 DEG C of water-bath lixiviate 1h, filter while hot, make Grape Skin leaching liquor stoste;
(3) by Grape Skin leaching liquor stoste dilute with water 5 times, to obtain final product.
The preparation of Preparative Example 5 grape leaf leaching liquor
(1) grape leaf is dried naturally, pulverize and sieve, keep in Dark Place;
(2) take grape leaf powder 1g, add water by the solid-liquid ratio of 1:8 (g:ml), at 20 DEG C of water-bath lixiviate 1h, filter while hot, make grape leaf leaching liquor stoste;
(3) by grape leaf leaching liquor stoste dilute with water 5 times, to obtain final product.
The preparation of embodiment 1 caragana compound cuttage root-taking agent
Each composition (unit: g): butyric acid potassium 6, lentinan (purchased from this high-new bio tech ltd of secret promise of Beijing, trade name: viral crack) 0.075, dehydro sodium acetate 0.2 is taken by following quality; Above-mentioned each composition is joined in water (distilled water) and supplies water to 100g, stir, to obtain final product.
The preparation of embodiment 2 caragana compound cuttage root-taking agent
Each composition (unit: g): butyric acid potassium 6, lentinan 0.075, dehydro sodium acetate 0.3 is taken by following quality; Above-mentioned each composition is joined in water (distilled water) and supplies water to 100g, stir, to obtain final product.
The preparation of embodiment 3 caragana compound cuttage root-taking agent
Each composition (unit: g): butyric acid potassium 9, lentinan 0.05, dehydro sodium acetate 0.3 is taken by following quality; Above-mentioned each composition is joined in water (distilled water) and supplies water to 100g, stir, to obtain final product.
The preparation of embodiment 4 caragana compound cuttage root-taking agent
Each composition (unit: g): butyric acid potassium 12, lentinan 0.05, dehydro sodium acetate 0.3 is taken by following quality; Above-mentioned each composition is joined in water (distilled water) and supplies water to 100g, stir, to obtain final product.
The preparation of embodiment 5 caragana compound cuttage root-taking agent
Each composition (unit: g): butyric acid potassium 9, lentinan 0.075, dehydro sodium acetate 0.3 is taken by following quality; Above-mentioned each composition is joined in water (distilled water) and supplies water to 100g, stir, to obtain final product.
The preparation of embodiment 6 caragana compound cuttage root-taking agent
The preparation of composition I: take each composition (unit: g): butyric acid potassium 9, lentinan 0.075, dehydro sodium acetate 0.3 by following quality; Above-mentioned each composition is joined in water (distilled water) and supplies water to 100g, stir, to obtain final product.
The preparation of composition II: grape branch leaching liquor prepared by Preparative Example 2.
Composition I and composition II are mixed according to the mass ratio of 1:15000, to obtain final product.
The preparation of embodiment 7 caragana compound cuttage root-taking agent
The preparation of composition I: take each composition (unit: g): butyric acid potassium 12, lentinan 0.1, dehydro sodium acetate 0.4 by following quality; Above-mentioned each composition is joined in water (distilled water) and supplies water to 100g, stir, to obtain final product.
The preparation of composition II: grape branch leaching liquor prepared by Preparative Example 2.
Composition I and composition II are mixed according to the mass ratio of 1:20000, to obtain final product.
The preparation of embodiment 8 caragana compound cuttage root-taking agent
The preparation of composition I: take each composition (unit: g): butyric acid potassium 12, lentinan 0.075, dehydro sodium acetate 0.3 by following quality; Above-mentioned each composition is joined in water (distilled water) and supplies water to 100g, stir, to obtain final product.
The preparation of composition II: grape branch leaching liquor prepared by Preparative Example 3.
Composition I and composition II are mixed according to the mass ratio of 1:15000, to obtain final product.
The screening test of the constituent of test example 1 compound caragana cuttage root-taking agent
One, test method
(1) the formula composition of each process compound cuttage root-taking agent
According to primary screening test, find the materials such as butyric acid potassium, lentinan, dehydro sodium acetate and the composite effect that there is hestening rooting and germinate of grape branch leaching liquor;
The compositions such as butyric acid potassium and heteroauxin, Nafusaku, lentinan, dehydro sodium acetate, grape branch leaching liquor, Grape Skin leaching liquor, grape leaf leaching liquor are carried out according to the mode of table 1 the compound caragana cuttage root-taking agent that compatibility obtains different disposal by this test.
Table 1 respectively processes the formula composition of compound cuttage root-taking agent and prepares and extension rate
(2) sand culture test
Get clean river sand tap water number time, then with the clean also 135 DEG C of high-temperature sterilization 2h of distilled water flushing.Naturally, after cooling, weigh and load in flowerpot.This test selects caragana spray to be test material, test totally 14 process (see table 1), distilled water is blank, often kind of cuttage root-taking agent all carries out unified immersion treatment (after spray sterilization, after the cuttage root-taking agent of each process converts dilution by extension rate, soak cuttings root 3-4cm; 2h is soaked at 20 DEG C of-30 DEG C of temperature), repeat for 4 times, random district group arranges.Each process 100 strain, after inserting, water spray, sets up shade net, keeps stem stalk moistening, avoids because bed surface temperature is too high and the dehydration of stem stalk is become dry.After cuttage, 45d dichotomy gets 50 strain cuttings, measures young sprout length, root length, root number, statistics survival rate.
(3) Drought resistant experiment
Caragana spray is selected to carry out Drought resistant experiment.Test totally 14 process (see table 1), unified immersion treatment (same to sand culture test) is all carried out in often kind of cuttage root-taking agent.Rinsed well by cuttage seeding root system after being cultured to 45d, be planted in flowerpot, and move into outdoor, grow under field conditions (factors), rainfall time shift enters indoor.After each normal plants, Severe drought (maxmun field capacity is 21.08%, arrange that soil moisture content is field capacity 35%) is set.The strain of every basin 10, repeat for 4 times, random district group arranges.The gross weight of each process flowerpot and plant is determined by weight method, after reaching cleanup standard, in pelvic surface of sacrum covered with plastic film, prevent moisture from evaporating, continue to weigh every day, fine day illumination, rainy day hides, supplement plant water consumption on daytime with gardens universal nutrient solution when every day afternoon 6, make each process be stabilized in the soil moisture content of design, after 60d, measure young sprout length, root is long.
(4) Salt tolerance
Caragana spray is selected to carry out Salt tolerance.Test totally 14 process (see table 1), unified immersion treatment (same to sand culture test) is all carried out in often kind of cuttage root-taking agent.Rinsed well by cuttage seeding root system after being cultured to 45d, be planted in flowerpot, and move into outdoor, grow under field conditions (factors), rainfall time shift enters indoor.With the gardens universal nutrient solution supply plant nutrition containing 100mmol/L sodium chloride, can normal growth.The strain of every basin 10, repeat for 4 times, random district group arranges, and measures young sprout length, root is long after 60d.
Two, result of the test
Sand culture test the results are shown in Table 2-table 7, and resistance test (drought resisting, salt tolerant) the results are shown in Table 8-table 12.
Result of the test shows, by butyric acid potassium, lentinan, cuttage root-taking agent (process 1) after dehydro sodium acetate and grape branch leaching liquor compatibility, compared to contrast (process 14), the long increase by 55.65% of young sprout of cuttage seeding, the long increase by 132.18% of root, root number increases by 229.29%, rotting rate reduces 416.5%, survival rate increases by 484.17%, drought resistance strengthens, and (the long growth rate of young sprout increases by 320%, the long growth rate of root increases by 540%), salt resistance strengthens, and (the long growth rate of young sprout increases by 333%, the long growth rate of root increases by 520%).Butyric acid potassium stimulates and effectively can be alleviated by lentinan, dehydro sodium acetate and grape branch leaching liquor the harmful effect of root, and it is remained valid to the facilitation of cutting.
Visible according to result of the test, by arranging in pairs or groups after butyric acid potassium, lentinan, dehydro sodium acetate compatibility, grape branch leaching liquor uses, urge strong at the growth of promotion young sprout, short root, the process that the aspects such as increase resistance are better than other (namely processes 2-14, difference reaches pole significance level), this illustrates, has Synergistic effect extremely significantly by compatible to butyric acid potassium and lentinan, dehydro sodium acetate and grape branch leaching liquor.
Table 2 sand culture test result
| Process | The long cm of young sprout | The long cm of root | Root number | Rotting rate % | Survival rate % |
| 1 | 17.9±0.32Aa | 6.71±0.15Aa | 12.48±0.53Aa | 15.2±0.46Hi | 25.47±0.12Aa |
| 2 | 12.0±0.37CDEdefgh | 4.95±0.01Cd | 9.3±0.29CDc | 20.95±0.57Ggh | 16.67±0.64Df |
| 3 | 12.72±0.7BCDbcdef | 5.73±0.19Bbc | 9.44±0.31BCc | 19.63±0.95Gh | 19.8±0.78Bd |
| 4 | 12.88±1.32BCDbcd | 5.91±0.19Bb | 9.69±0.37BCbc | 28.32±0.93CDcd | 20.79±0.64Bb |
| 5 | 12.93±0.96BCDbcd | 4.91±0.18Cd | 8.47±0.09Ed | 26.56±1.08DEde | 16.47±0.09Df |
| 6 | 13.63±0.24Bb | 5.57±0.31Bc | 9.47±0.26BCc | 29.97±1.15Cc | 20.62±0.21Bbc |
| 7 | 13.63±0.79Bb | 5.6±0.31Bc | 9.7±0.32BCbc | 24.26±0.78EFef | 19.88±0.5Bcd |
| 8 | 13.24±1.14BCbc | 4.88±0.04Cd | 8.49±0.33Ed | 27.02±1.37CDEde | 17.21±0.79CDef |
| 9 | 11.71±0.74DEfgh | 5.09±0.15Cd | 9.97±0.31Bb | 30.01±1.06Cc | 20.79±0.33Bb |
| 10 | 10.97±0.21Eh | 3.13±0.15Ef | 6.42±0.5Ff | 48.66±1.13Bb | 8.68±0.33Eg |
| 11 | 12.2±1.3BCDEcdefg | 3.94±0.15De | 8.71±0.39DEd | 20.47±0.34Ggh | 17.85±0.92Ce |
| 12 | 12.85±0.63BCDbcde | 3.66±0.41De | 6.58±0.3Fef | 22.62±1.58FGfg | 16.92±0.82CDf |
| 13 | 11.75±0.33DEefgh | 4.85±0.13Cd | 6.92±0.34Fe | 22.29±1.44FGfgh | 17.1±0.47CDef |
| 14 | 11.5±0.97DEgh | 2.89±0.08Ef | 3.79±0.17GFg | 78.51±1.35Aa | 4.36±0.21Fh |
The long variance analysis table of table 3 sand culture test young sprout
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| Between district's group | 3.1464 | 3 | 1.0488 | 1.721 | 0.1785 |
| Between process | 143.528 | 13 | 11.0406 | 18.118 | 0.0000 |
| Error | 23.7654 | 39 | 0.6094 | ||
| Total variation | 170.4397 | 55 |
The long variance analysis table of table 4 sand culture test root
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| Between district's group | 0.1196 | 3 | 0.0399 | 0.959 | 0.4217 |
| Between process | 62.2209 | 13 | 4.7862 | 115.113 | 0.0000 |
| Error | 1.6216 | 39 | 0.0416 | ||
| Total variation | 63.9621 | 55 |
Table 5 sand culture test root number variance analysis table
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| Between district's group | 0.1871 | 3 | 0.0624 | 0.519 | 0.6715 |
| Between process | 224.1444 | 13 | 17.2419 | 143.588 | 0.0000 |
| Error | 4.6831 | 39 | 0.1201 | ||
| Total variation | 229.0146 | 55 |
Table 6 sand culture test rotting rate variance analysis table
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| Between district's group | 0.621 | 3 | 0.207 | 0.168 | 0.9172 |
| Between process | 13476.52 | 13 | 1036.655 | 842.501 | 0.0000 |
| Error | 47.9876 | 39 | 1.2305 |
| Total variation | 13525.13 | 55 |
Table 7 sand culture test survival rate variance analysis table
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| Between district's group | 1.806 | 3 | 0.602 | 2.081 | 0.1184 |
| Between process | 1433.226 | 13 | 110.2481 | 381.159 | 0.0000 |
| Error | 11.2805 | 39 | 0.2892 | ||
| Total variation | 1446.312 | 55 |
Table 8 drought resisting, Salt tolerance result
The long relative growth rate variance analysis table of table 9 Drought resistant experiment young sprout
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| Between district's group | 0.0003 | 3 | 0.0001 | 0.393 | 0.7585 |
| Between process | 0.1204 | 13 | 0.0093 | 39.474 | 0.0000 |
| Error | 0.0091 | 39 | 0.0002 |
| Total variation | 0.1298 | 55 |
The long relative growth rate variance analysis table of table 10 Drought resistant experiment root
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| Between district's group | 0.002 | 3 | 0.0007 | 9.448 | 0.0001 |
| Between process | 0.5565 | 13 | 0.0428 | 619.965 | 0.0000 |
| Error | 0.0027 | 39 | 0.0001 | ||
| Total variation | 0.5611 | 55 |
The long relative growth rate variance analysis table of table 11 Salt tolerance young sprout
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| Between district's group | 0.0007 | 3 | 0.0002 | 1.011 | 0.3983 |
| Between process | 0.0451 | 13 | 0.0035 | 14.594 | 0.0000 |
| Error | 0.0093 | 39 | 0.0002 | ||
| Total variation | 0.0551 | 55 |
The long relative growth rate variance analysis table of table 12 Salt tolerance root
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| Between district's group | 0.0023 | 3 | 0.0008 | 13.081 | 0.0000 |
| Between process | 0.3474 | 13 | 0.0267 | 454.533 | 0.0000 |
| Error | 0.0023 | 39 | 0.0001 | ||
| Total variation | 0.352 | 55 |
The inspection test of the constituent of test example 2 caragana compound cuttage root-taking agent
One, test method
(1) the formula composition of each process compound cuttage root-taking agent
The result of the constituent screening test of test example 1 shows, the harmful effect of butyric acid potassium to root system can be eliminated after lentinan, dehydro sodium acetate and grape branch leaching liquor are composite, and survival rate is higher, effect of butyric acid potassium and lentinan is not fully exerted.In view of the impact between plant growth regulator is very complicated, there is the multiple impact such as synergy, antagonism, the situation that binary built is better than ternary built may be there is.In addition, from the viewpoint of cost and preparation processing, reduce medicament kind and also have obvious advantage, so design binary built and ternary built test according to the situation of test example 1, the consumption with reference to table 13 designs.
Table 13 respectively processes the formula composition of compound cuttage root-taking agent and prepares and extension rate
(2) sand culture test
Concrete grammar is with test example 1.
(3) Drought resistant experiment
Concrete grammar is with test example 1.
(4) Salt tolerance
Concrete grammar is with test example 1.
Two, result of the test
Result of the test is in Table 14-table 22.
Visible according to result of the test, the compound scheme that the composite Be very effective of butyric acid potassium, lentinan, dehydro sodium acetate and leaching liquor is better than other (namely processes 2-7, difference reaches pole significance level), binding tests example 1 considers, the composite effect of butyric acid potassium, lentinan and dehydro sodium acetate obviously will be better than other compound scheme, and this shows there is obvious Synergistic effect between three kinds of materials in this compound scheme and grape branch leaching liquor.
Table 14 sand culture test result
| Process | The long cm of young sprout | The long cm of root | Survival rate % |
| 1 | 17.71±0.24Aa | 6.59±0.35Aa | 21.36±0.64Aa |
| 2 | 14.5±0.44Bb | 4.47±0.08Bb | 20.01±0.71Bb |
| 3 | 12.47±0.35Dd | 3.67±0.54Cc | 16.53±0.78Dd |
| 4 | 11.58±0.25Ee | 3.73±0.52Cc | 17.26±0.56CDd |
| 5 | 13.58±0.25Cc | 4.75±0.18Bb | 18.5±0.66Cc |
| 6 | 11.1±0.35EFf | 3.68±0.41Cc | 16.96±0.35Dd |
| 7 | 10.6±0.32Fg | 2.86±0.08Dd | 4.4±0.28Ee |
The long variance analysis table of table 15 sand culture test young sprout
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| Between district's group | 0.3763 | 3 | 0.1254 | 1.29 | 0.3083 |
| Between process | 145.7707 | 6 | 24.2951 | 249.789 | 0.0000 |
| Error | 1.7507 | 18 | 0.0973 | ||
| Total variation | 147.8977 | 27 |
The long variance analysis table of table 16 sand culture test root
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| Between district's group | 1.3356 | 3 | 0.4452 | 5.772 | 0.006 |
| Between process | 34.5514 | 6 | 5.7586 | 74.666 | 0.0000 |
| Error | 1.3882 | 18 | 0.0771 | ||
| Total variation | 37.2752 | 27 |
Table 17 sand culture test survival rate analytical table
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| Between district's group | 0.1081 | 3 | 0.036 | 0.089 | 0.9652 |
| Between process | 747.8421 | 6 | 124.6404 | 307.458 | 0.0000 |
| Error | 7.297 | 18 | 0.4054 | ||
| Total variation | 755.2473 | 27 |
Table 18 drought resisting, Salt tolerance result
The long relative growth rate variance analysis table of table 19 Drought resistant experiment young sprout
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| Between district's group | 0.0007 | 3 | 0.0002 | 0.705 | 0.5614 |
| Between process | 0.0717 | 6 | 0.0119 | 38.456 | 0.0000 |
| Error | 0.0056 | 18 | 0.0003 | ||
| Total variation | 0.0779 | 27 |
The long relative growth rate variance analysis table of table 20 Drought resistant experiment root
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| Between district's group | 0.0004 | 3 | 0.0001 | 1.949 | 0.1579 |
| Between process | 0.3116 | 6 | 0.0519 | 739.441 | 0.0000 |
| Error | 0.0013 | 18 | 0.0001 | ||
| Total variation | 0.3133 | 27 |
The long relative growth rate variance analysis table of table 21 Salt tolerance young sprout
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| Between district's group | 0.0021 | 3 | 0.0007 | 17 | 0.0000 |
| Between process | 0.071 | 6 | 0.0118 | 283.972 | 0.0000 |
| Error | 0.0007 | 18 | 0 | ||
| Total variation | 0.0739 | 27 |
The long relative growth rate variance analysis table of table 22 Salt tolerance root
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| Between district's group | 0 | 3 | 0 | 0.049 | 0.9852 |
| Between process | 0.2784 | 6 | 0.0464 | 635.413 | 0.0000 |
| Error | 0.0013 | 18 | 0.0001 | ||
| Total variation | 0.2797 | 27 |
The consumption screening test of the constituent of test example 3 caragana compound cuttage root-taking agent
One, test method
(1) the consumption design of the constituent of caragana compound cuttage root-taking agent
According to consumption design 15 processed group of table 23.
Table 23 respectively processes the compound method of cuttage root-taking agent
(2) sand culture test
Concrete grammar is with test example 1.
(3) Drought resistant experiment
Concrete grammar is with test example 1.
(4) Salt tolerance
Concrete grammar is with test example 1.
Two, result of the test
Result of the test is in Table 24-table 31.
Visible according to result of the test, the good compound scheme of effect is: butyric acid potassium 6%-12%+ lentinan 0.05%-0.1%+ dehydro sodium acetate 0.2%-0.4%+ grape branch leaching liquor (grape branch leaching liquor stoste dilutes 5 times); This compound scheme is better than extraneous other scheme (difference reaches significance level) of this compound scheme at promotion root growth, resistance Be very effective; Wherein, the effect of butyric acid potassium 9%+ lentinan 0.075%+ dehydro sodium acetate 0.3%+ grape branch leaching liquor (grape branch leaching liquor stoste dilutes 5 times) is best in compound scheme, is significantly better than other formula (difference reaches pole significance level) in this compound scheme.
Table 24 sand culture test result
| Process | The long cm of young sprout | The long cm of root |
| 1 | 17.5±0.32Aa | 6.6±0.33Aa |
| 2 | 13.21±0.36Bb | 5.3±0.17Bb |
| 3 | 11.16±0.35Cc | 3.29±0.19BCbc |
| 4 | 13.33±0.3Cc | 5.35±0.13BCbc |
| 5 | 11.4±0.52Dd | 3.46±0.26BCc |
| 6 | 10.39±0.52Dd | 3.4±0.12BCc |
| 7 | 14.61±0.33Dd | 5.47±0.4Cc |
| 8 | 13.41±0.11DEd | 5.61±0.32Cc |
| 9 | 14.59±0.54EFe | 5.61±0.32Dd |
| 10 | 13.67±0.41Fe | 5.49±0.45Dd |
| 11 | 16.13±0.58Gf | 5.89±0.08Ee |
| 12 | 12.57±0.23Gf | 4.37±0.26Ee |
| 13 | 12.45±0.39Gf | 4.17±0.2Ee |
| 14 | 11.35±0.26Gf | 3.32±0.17Ee |
| 15 | 11.28±0.28Hg | 3.41±0.27Ee |
The long variance analysis table of table 25 sand culture test young sprout
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| Between district's group | 0.3178 | 3 | 0.1059 | 0.688 | 0.5645 |
| Between process | 218.3349 | 14 | 15.5953 | 101.259 | 0.0000 |
| Error | 6.4686 | 42 | 0.154 | ||
| Total variation | 225.1213 | 59 |
The long variance analysis table of table 26 sand culture test root
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| Between district's group | 0.8983 | 3 | 0.2994 | 5.517 | 0.0028 |
| Between process | 71.4669 | 14 | 5.1048 | 94.059 | 0.0000 |
| Error | 2.2794 | 42 | 0.0543 | ||
| Total variation | 74.6447 | 59 |
Table 27 drought resisting, Salt tolerance result
The long relative growth rate variance analysis table of table 28 Drought resistant experiment young sprout
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| Between district's group | 0.0006 | 3 | 0.0002 | 1.972 | 0.1329 |
| Between process | 0.1297 | 14 | 0.0093 | 84.939 | 0.0000 |
| Error | 0.0046 | 42 | 0.0001 | ||
| Total variation | 0.1349 | 59 |
The long relative growth rate variance analysis table of table 29 Drought resistant experiment root
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| Between district's group | 0.0006 | 3 | 0.0002 | 2.377 | 0.0834 |
| Between process | 0.1686 | 14 | 0.012 | 149.813 | 0.0000 |
| Error | 0.0034 | 42 | 0.0001 | ||
| Total variation | 0.1726 | 59 |
The long relative growth rate variance analysis table of table 30 Salt tolerance young sprout
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| Between district's group | 0.0006 | 3 | 0.0002 | 1.921 | 0.1409 |
| Between process | 0.0956 | 14 | 0.0068 | 63.621 | 0.0000 |
| Error | 0.0045 | 42 | 0.0001 | ||
| Total variation | 0.1007 | 59 |
The long relative growth rate variance analysis table of table 31 Salt tolerance root
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| Between district's group | 0.0005 | 3 | 0.0002 | 2.367 | 0.0844 |
| Between process | 0.1765 | 14 | 0.0126 | 174.418 | 0.0000 |
| Error | 0.003 | 42 | 0.0001 | ||
| Total variation | 0.1801 | 59 |
Claims (10)
1. a caragana compound cuttage root-taking agent, is characterized in that, is grouped into by following one-tenth: butyric acid potassium, lentinan, dehydro sodium acetate and water.
2. according to caragana compound cuttage root-taking according to claim 1 agent, it is characterized in that: by mass percentage, the consumption of each composition is: butyric acid potassium 6-12%, lentinan 0.05-0.1%, dehydro sodium acetate 0.2-0.4%, surplus are water; Preferably, the consumption of each composition is: butyric acid potassium 9%, lentinan 0.075%, dehydro sodium acetate 0.3%.
3. prepare a method for caragana compound cuttage root-taking agent described in claim 1 or 2, it is characterized in that, the method comprises: butyric acid potassium, lentinan and dehydro sodium acetate are added to the water, and stirring and dissolving, to obtain final product.
4. a caragana compound cuttage root-taking agent, is characterized in that: be made up of composition I and composition II; Wherein, described composition I is made up of butyric acid potassium, lentinan, dehydro sodium acetate and water; Described composition II is grape branch leaching liquor.
5. according to caragana compound cuttage root-taking according to claim 4 agent, it is characterized in that: by mass percentage, in composition I, the consumption of each composition is: butyric acid potassium 6-12%, lentinan 0.05-0.1%, dehydro sodium acetate 0.2-0.4%, surplus are water; Preferably, the consumption of each composition is: butyric acid potassium 9%, lentinan 0.075%, dehydro sodium acetate 0.3%;
According to mass ratio meter, composition I: composition II=1:10000-20000; Preferably, composition I: composition II=1:12500-17500; Most preferred, composition I: composition II=1:15000.
6. according to caragana compound cuttage root-taking according to claim 4 agent, it is characterized in that: the preparation of described grape branch leaching liquor comprises: kyoto grape branch flooding is obtained grape branch lixiviate stoste; By grape branch lixiviate stoste dilute with water, to obtain final product; Preferably, by grape branch leaching liquor stoste dilute with water 2-20 doubly, preferred, by grape branch leaching liquor stoste dilute with water 2-8 doubly, most preferred, by grape branch leaching liquor stoste dilute with water 5 times.
7., according to caragana compound cuttage root-taking according to claim 6 agent, it is characterized in that:
Described grape branch lixiviate stoste prepares in accordance with the following methods:
(1) collect the grape winter and cut bar, natural seasoning;
(2) be added to the water after being pulverized by the branch of natural seasoning lixiviate, to obtain final product;
Preferably, in step (2), be added to the water after the branch of natural seasoning is pulverized, 15-25 DEG C of water-bath lixiviate 0.5-3h; Wherein according to g:ml meter, grape branch powder: water=1:9.
8. prepare a method for the caragana compound cuttage root-taking agent described in claim 4 or 5, it is characterized in that, comprising: composition I and composition II are mixed, to obtain final product.
9. caragana compound cuttage root-taking agent described in claim 1 or 2 is in the application promoting caragana cuttage rooting, improve the survival rate of cuttings, improve biocidal property or promote in resistance; Preferably, described application comprises: by described caragana compound cuttage root-taking agent dilute with water, obtains the caragana compound cuttage root-taking agent after diluting; Caragana compound cuttage root-taking agent after caragana cuttings being diluted is soaked; Preferably, by described caragana compound cuttage root-taking agent dilute with water 10000-20000 doubly, be more preferably 12500-17500 doubly, most preferably be 15000 times, obtain the caragana compound cuttage root-taking agent after diluting; Wherein, cuttings biology bottom 3-4cm is soaked with caragana taking root liquid after the dilution of grape branch leaching liquor; Described immersion preferably soaks 2h at 20-30 DEG C of temperature.
10. claim 4-7 caragana compound cuttage root-taking agent described in any one is in the application promoting caragana cuttage rooting, improve survival rate, improve biocidal property or promote in resistance; Preferably, described application comprises: the described caragana compound cuttage root-taking agent of caragana cuttings soaked; Wherein, after mixing with grape branch leaching liquor, soak cuttings biology bottom 3-4cm with caragana taking root liquid; Described immersion preferably soaks 2h at 20-30 DEG C of temperature.
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| CN111296451A (en) * | 2020-04-07 | 2020-06-19 | 江西瑞博特生物科技有限公司 | Rooting agent for cutting seedling as well as preparation method and application thereof |
| CN114009437A (en) * | 2021-09-01 | 2022-02-08 | 天津市汉邦植物保护剂有限责任公司 | Granules containing indolebutyric acid and lentinan, and preparation method and application thereof |
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| CN106070326A (en) * | 2016-06-11 | 2016-11-09 | 徐伟明 | A kind of special powder and cottage method thereof of taking root of Huanghua Pear |
| CN111296451A (en) * | 2020-04-07 | 2020-06-19 | 江西瑞博特生物科技有限公司 | Rooting agent for cutting seedling as well as preparation method and application thereof |
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| CN114009437A (en) * | 2021-09-01 | 2022-02-08 | 天津市汉邦植物保护剂有限责任公司 | Granules containing indolebutyric acid and lentinan, and preparation method and application thereof |
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