CN105145603B - Compound wheat seed soaking agent and preparation method thereof, application method and application - Google Patents
Compound wheat seed soaking agent and preparation method thereof, application method and application Download PDFInfo
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- CN105145603B CN105145603B CN201510500471.3A CN201510500471A CN105145603B CN 105145603 B CN105145603 B CN 105145603B CN 201510500471 A CN201510500471 A CN 201510500471A CN 105145603 B CN105145603 B CN 105145603B
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Abstract
The invention discloses compound wheat seed soaking agent and preparation method thereof, application method and application, belongs to wheat seed soaking agent field.The compound wheat seed soaking agent of the present invention mainly includes uniconazole P, Bachmedesh and acardite.The present invention further discloses preparation method, application method and its application of the compound wheat seed soaking agent.The present invention can more effectively eliminate uniconazole P and Bachmedesh, acardite progress ternary built the counter productive using single component with zasiokaurin liquid dilution post processing wheat seed again, four kinds of compositions are compatible to have the effect of extremely significant Synergistic, seedling after sprouting is short strong, well developed root system, lodging resistance strengthens, and the anti-adversity such as insect resistace, drought resistance and salt tolerance is obviously improved.The compound wheat seed soaking agent processing wheat seed of the present invention can expand wheat planting scope, and with volume increase, stable yields and other effects.
Description
Technical field
The present invention relates to seed soaking agent, more particularly to a kind of suitable wheat with various plants growth regulator and zasiokaurin
Liquid promotees to strengthen and improves lodging resistance, resistance etc. for active ingredient, in promoting root growth the compound wheat leaching of notable synergy effect
Kind agent, belongs to wheat seed soaking agent field.
Background technology
Wheat is one of main cereal crops of China.Wheat is easily encroached in kind of seedling stage by adverse circumstances such as arid, salt damagies, growth
Easily there is lodging problem in later stage.At present, educate strong sprout and it is resistant to lodging be to expand wheat planting scope, volume increase, the important means of stable yields.
To solve the above problems, in addition to field management measure, common method is seed pelleting, seed dressing and seed soaking.Adopt
Seed is handled with seed soaking agent, simple production process, easy to use, treating capacity is big, seed can not only be allowed to fully absorb various effective
The effect that composition, also vernalization are early sent out.
CN104286025 A disclose a kind of composite efficient wheat seed soaking agent and its application method, involved plant
Growth regulator is uniconazole P and indolebutyric acid, has certain effect in terms of wheat growth-promoting, does not improve wheat and resist
The effect of resistance such as worm property.CN104012534 A disclose a kind of wheat cold-resistant conditioning agent, involved plant growth regulating
Agent is salicylic acid and brassin lactones, and it is barely satisfactory that the conditioning agent in promoting root growth promotees strong, increase wheat lodging resistance etc., needs
Improve.CN104186065 A disclose a kind of degeneration-resistant seed-soaking method of wheat strong sprout, and involved plant growth regulator is short
Strong element, it does not have effect substantially in terms of growth of wheat roots is promoted.
In order to overcome many defects present in existing wheat seed soaking agent, promote growth of wheat roots, improve Wheat Species
The lodging resistance of seedling stage stress resistance and Later growth, it would be highly desirable to need to develop it is a kind of can effectively promoting root growth promote it is strong, increase wheat anti-fall
The compound wheat seed soaking agent of property and resistance.
The content of the invention
The technical problems to be solved by the invention are overcome the deficiencies in the prior art, there is provided one kind effectively promoting root growth can promote
Strong, increase lodging resistance and the compound wheat seed soaking agent for improving the anti-adversities such as insect resistace, drought resistance and salt tolerance.
The technical problems to be solved by the invention are achieved through the following technical solutions：
A kind of compound wheat seed soaking agent, is made up of following composition：Uniconazole P, Bachmedesh, acardite and acetic acid.
Preferably, by mass percentage, the dosage of each composition is：Uniconazole P 0.4%-0.5%, Bachmedesh 1.2%-
1.5%th, acardite 0.5%-0.6%, surplus are acetic acid；
It is furthermore preferred that the dosage of each composition is：Uniconazole P 0.5%, Bachmedesh 1.5%, acardite 0.5%.
Uniconazole P is a kind of plant growth regulator for having and downgrading and promoting strong effect, can effectively reduce plant height so as to improve
Lodging resistance, but uniconazole P is used alone and has the counter productive that speed of germination reduces, root long shortens.Single plant is used in order to eliminate
The counter productive of thing growth regulator, the present invention have uniconazole P and DCPTA, diethyl aminoethyl hexanoate, 6-BA, Bachmedesh, acardite etc.
The composition for having hestening rooting to germinate is compatible, it is expected that screening obtains one kind and can not only effectively reduce plant height, additionally it is possible to effective promoting root growth
Promote the compound wheat seed soaking agent of strong increase lodging resistance.The present invention is had found by screening experiment, by uniconazole P and Bachmedesh, two
After the compound wheat seed soaking agent collocation zasiokaurin liquid use that phenylurea is compounded to obtain, reduce plant height, hestening rooting and
Other compounded combinations are significantly better than in insect resistace, drought resistance, salt tolerance etc..By uniconazole P and Bachmedesh, hexichol
Urea is compounded, and can effectively eliminate the counter productive using single medicament, and suppression of the uniconazole P to plant height is not failed and overcome
The defects of Bachmedesh and acardite make plant height excessively rapid growth, suppression of the uniconazole P to root growth effectively by Bachmedesh and
Acardite is cleared up, and three's compatibility significantly enhances the resistance of wheat again together, has extremely significant Synergistic effect.
Influence between plant growth regulator is extremely complex, it is understood that there may be a variety of influences such as synergy, antagonism, can so that having
That binary built is better than ternary built can be present.In addition, from the aspect of cost and preparation processing, medicament kind is reduced
With obvious advantage；In consideration of it, further the experiment of design binary built is further to the effect work of ternary built by the present invention
Inspection.Result of the test is visible, and uniconazole P, Bachmedesh and acardite carry out the significant effect of ternary built better than others two
First compound scheme (difference reaches the pole level of signifiance)；Composite score is visible, the compounding effect of uniconazole P, Bachmedesh and acardite
Fruit is intended to be substantially better than than other compound schemes, confirms uniconazole P and Bachmedesh and acardite carrying out compounding with notable
Synergistic effect.
It is being determined that compound wheat seed soaking agent is made up of the basis with Synergistic uniconazole P, Bachmedesh and acardite
On, the present invention is it has furthermore been found that the different amounts proportioning of uniconazole P, Bachmedesh and acardite, the influence for effect of soaking seed
Also highly significant.The present invention has found that three kinds of compositions carry out compatibility according to following amount ranges to be had by substantial amounts of screening experiment
Preferable synergy：
By mass percentage, uniconazole P 0.4%-0.5%, Bachmedesh 1.2%-1.5%, acardite 0.5%-
0.6%th, surplus is acetic acid；
Wherein, three kinds of compositions carry out compatibility according to following dosage has best synergy：
Uniconazole P 0.5%, Bachmedesh 1.5%, acardite 0.5%, surplus are acetic acid.
Invention further provides a kind of method for preparing the compound wheat seed soaking agent, this method includes：Alkene is imitated
Azoles, Bachmedesh and acardite are added in acetic acid, are stirred, and are produced.
In order to reach more preferably technique effect, described acetic acid is preferably the acetic acid of content >=75%.
The present invention still further provides the compound wheat seed soaking agent reduce Plant Height in Wheat, promoting root growth promotees that strong, increase is anti-
The application in property and raising resistance aspect, including：The compound wheat seed soaking agent is diluted with water, answering after being diluted
Close wheat seed soaking agent；Wheat seed is soaked seed with the compound wheat seed soaking agent after dilution；
Wherein, 5000-15000 times, preferably 8000-12000 times is diluted with water in the compound wheat seed soaking agent, it is more excellent
Elect 10000 times as；
According to mass ratio meter, the ratio of wheat seed and the compound wheat seed soaking agent liquid after dilution is preferably 1:3；Described
Immersion is preferably soaked seed 24 hours at a temperature of 20 DEG C -30 DEG C.
The present invention is found surprisingly that very much, after uniconazole P, Bachmedesh, acardite and zasiokaurin liquid phase compatibility, energy
The raising seed soaking agent of enough highly significants promotees strong, increase lodging resistance in promoting root growth and improves insect resistace, drought resistance and salt tolerance etc.
Effect in terms of resistance.
Zasiokaurin contains protein 30.43%, fat 2.5%, starch 22.4%, reduced sugar 6.88%, non-reducing sugar
7.31%th, nucleic acid 1.63%.Contain the kinds more than 20 such as 10 multivitamins, calcium, iron, potassium, sodium such as Va, Vb, Vc, Ve in zasiokaurin
The material such as 18 kinds of amino acid such as mineral element, proline, asparatate, glutamic acid, lysine and flavone compound.
North China of China, Wheat Maize Rotation are the common tillage methods of wheat planting district.Zasiokaurin is rich in multiple beneficial thing
Matter, pollen liquid is made after zasiokaurin is collected, the wheat seed of stubble sowing is connect for handling, arrangement of time is reasonable, moreover it is possible to becomes
Waste be changed into values.The speed of growth of wheat can be improved by carrying out seed soaking to wheat seed using zasiokaurin liquid, but is used alone beautiful
Rice pollen liquid, which makees seed soaking, can reduce the insect resistace and the lodging resistance in later stage of wheat (plant height increases too fast).
In order to screen the optimum amount of uniconazole P, Bachmedesh, acardite and zasiokaurin liquid proportioning, the present invention is to four kinds
The consumption proportion of constituent is screened, and more different dosages reduces plant height for wheat, promoting root growth promotees strong, increase lodging resistance
With the influence of resistance etc.；Result of the test shows that the preferable compound scheme of effect is uniconazole P 0.4%-0.5%+ chrysanthemum second
Amine ester 1.2%-1.5%+ acardite 0.5%-0.6%, with zasiokaurin liquid (zasiokaurin stoste dilutes 5 times) dilution 10000
Times, other consumption proportions will be significantly better than in terms of promoting root growth and improving resistance and other effects using the consumption proportion
(difference reaches the level of signifiance), wherein, uniconazole P 0.5%+ Bachmedesh 1.5%+ acardite 0.5%, with zasiokaurin liquid (corn
Pollen stoste dilutes 5 times) 10000 times of effect of dilution is that effect is best in all compound schemes, it is significantly better than the compounding side
Other consumption proportions in case (difference reaches the pole level of signifiance).
Therefore, present invention also offers another compound wheat seed soaking agent, it is made up of composition I and composition II；According to matter
Amount is than meter, composition I:Composition II=1:5000-15000；Preferably, composition I:Composition II=1:7500-12500；Most preferably,
Composition I:Composition II=1:10000.
The composition I is made up of uniconazole P, Bachmedesh, acardite and acetic acid；By mass percentage, it is each in composition I
The dosage of composition is：Uniconazole P 0.4%-0.5%, Bachmedesh 1.2%-1.5%, acardite 0.5%-0.6%, surplus are second
Acid；Preferably, the dosage of each composition is：Uniconazole P 0.5%, Bachmedesh 1.5%, acardite 0.5%；
The composition II is zasiokaurin liquid.
Described zasiokaurin liquid is obtained after zasiokaurin stoste is diluted with water, it is preferred that described zasiokaurin
Liquid is obtained after zasiokaurin stoste is diluted with water into 2-20 times；It is furthermore preferred that described zasiokaurin liquid is by zasiokaurin
Stoste obtains after being diluted with water 2-8 times；Most preferably, described zasiokaurin liquid is that zasiokaurin stoste is diluted with water into 5 times
After obtain.
The zasiokaurin stoste is preferably prepared in accordance with the following methods：
(1) zasiokaurin is gathered；
(2) zasiokaurin is freezed to storage at a temperature of -10 DEG C~-20 DEG C；
(3) zasiokaurin freezed after depositing is added in hot water and stirred, produced after cooling.
Preferably, zasiokaurin is gathered in corn full-bloom stage in step (1), by the zasiokaurin of same day collection in sunlight
Under be exposed to the sun 1-2 hours；
Preferably, zasiokaurin is freezed to storage at a temperature of -20 DEG C in step (2)；
Preferably, the zasiokaurin for freezing storage at least 30 days is added to the 80-100 of 3-20 times of quality in step (3)
Stir in DEG C hot water, produced after cooling；It is further preferred that the zasiokaurin for freezing storage at least 30 days is added to 10
Stirred in 100 DEG C of hot water of times quality.
The invention provides a kind of method for preparing the compound wheat seed soaking agent, including：Composition I and composition II are mixed
It is even, produce.
Accordingly, strong, increase lodging resistance is promoted in reduction Plant Height in Wheat, promoting root growth the invention provides the compound wheat seed soaking agent
Or the application in raising resistance aspect；The application includes：Wheat seed is soaked with described compound wheat seed soaking agent
Kind；Wherein, according to mass ratio meter, the ratio of wheat seed and compound wheat seed soaking agent liquid is preferably 1:3；Described immersion is excellent
Choosing is soaked seed 24 hours at a temperature of 20 DEG C -30 DEG C.
The compound wheat seed soaking agent of the present invention is compounded uniconazole P and Bachmedesh, acardite, then coordinates zasiokaurin
Liquid uses, and can effectively eliminate the counter productive using single medicament, and four compatibilities have extremely significant Synergistic work(together
Effect, after handling wheat seed with it, seedling short strong (plant height reduction 40%-60%, the wide increase 100%- of stem foot after sprouting
200%), well developed root system (root long increase 5%-15%, root bar number increase 10%-20%)；Effective promoting root growth promotees to strengthen, it is anti-fall to increase
Property, while resistance (percentage of injury reduces 75%-95%, drought resistance strengthens 1-2 times, 1-2 times of salt tolerance enhancing) is improved, finally reach
To expand planting range, volume increase, stable yields purpose.
Embodiment
The invention will now be further described with reference to specific embodiments, advantages of the present invention and feature will be with description and
It is apparent.It should be understood that the embodiment is only exemplary, any restrictions are not formed to the scope of the present invention.This area
Technical staff should be understood that without departing from the spirit and scope of the invention can to the details of technical solution of the present invention and
Form is modified or replaced, but these modifications or substitutions each fall within protection scope of the present invention.
The preparation of the zasiokaurin stoste of Preparative Example 1
(1) in the sunny morning (9. -11 point) of corn full-bloom stage, interval clip (interlacing or every strain) loose powder about 50%
Maize male ears, shakeout on clean plastic cloth or paper, be exposed to the sun in the sun 1-2 hours in noon on the same day (11. -14 point)；
(2) pollen is shaken to cause drop in concentrating harvesting on clean plastic cloth or paper, weeds out the impurity such as flower pesticide, load clean closed
Vessel, deposit in freezer compartment of refrigerator (temperature is adjusted to -20 DEG C)；
(3) the zasiokaurin taking-up for depositing 30 days will be freezed, rapidly joins 100 DEG C of hot water of 10 times of quality and stir equal
It is even, room temperature is naturally cooled to, obtains zasiokaurin stoste.
The preparation of the zasiokaurin stoste of Preparative Example 2
(1) in the sunny morning (9. -11 point) of corn full-bloom stage, interval clip (interlacing or every strain) loose powder about 50%
Maize male ears, shakeout on clean plastic cloth or paper, be exposed to the sun in the sun 2-3 hours in noon on the same day (11. -14 point)；
(2) pollen is shaken to cause drop in concentrating harvesting on clean plastic cloth or paper, weeds out the impurity such as flower pesticide, load clean closed
Vessel, deposit in freezer compartment of refrigerator (temperature is adjusted to -15 DEG C)；
(3) the zasiokaurin taking-up for depositing 40 days will be freezed, rapidly joins 90 DEG C of hot water of 6 times of quality and stir,
Room temperature is naturally cooled to, obtains zasiokaurin stoste.
The preparation of the zasiokaurin stoste of Preparative Example 3
(1) in the sunny morning (9. -11 point) of corn full-bloom stage, interval clip (interlacing or every strain) loose powder about 50%
Maize male ears, shakeout on clean plastic cloth or paper, be exposed to the sun in the sun 2-3 hours in noon on the same day (11. -14 point)；
(2) pollen is shaken to cause drop in concentrating harvesting on clean plastic cloth or paper, weeds out the impurity such as flower pesticide, load clean closed
Vessel, deposit in freezer compartment of refrigerator (temperature is adjusted to -10 DEG C)；
(3) the zasiokaurin taking-up for depositing 38 days will be freezed, rapidly joins 80 DEG C of hot water of 12 times of quality and stir,
Room temperature is naturally cooled to, obtains zasiokaurin stoste.
The preparation of the compound wheat seed soaking agent of embodiment 1
Each composition (unit is weighed by following quality：g)：Uniconazole P 0.5, Bachmedesh 1.5, acardite 0.5；Will be above-mentioned each
Composition, which is added in acetic acid (content >=75%), supplies acetic acid to 100g, stirs, produces.
The preparation of the compound wheat seed soaking agent of embodiment 2
Each composition (unit is weighed by following quality：g)：Uniconazole P 0.4, Bachmedesh 1.2, acardite 0.5；Will be above-mentioned each
Composition, which is added in acetic acid, supplies acetic acid (content >=75%) to 100g, stirs, produces.
The preparation of the compound wheat seed soaking agent of embodiment 3
Each composition (unit is weighed by following quality：g)：Uniconazole P 0.5, Bachmedesh 1.5, acardite 0.6；Will be above-mentioned each
Composition, which is added in acetic acid, supplies acetic acid (content >=75%) to 100g, stirs, produces.
The preparation of the compound wheat seed soaking agent of embodiment 4
Each composition (unit is weighed by following quality：g)：Uniconazole P 0.4, Bachmedesh 1.5, acardite 0.6；Will be above-mentioned each
Composition, which is added in acetic acid, supplies acetic acid (content >=75%) to 100g, stirs, produces.
The preparation of the compound wheat seed soaking agent of embodiment 5
Each composition (unit is weighed by following quality：g)：Uniconazole P 0.5, Bachmedesh 1.2, acardite 0.5；Will be above-mentioned each
Composition, which is added in acetic acid, supplies acetic acid (content >=75%) to 100g, stirs, produces.
The preparation of the compound wheat seed soaking agent of embodiment 6
The preparation of composition I：Each composition (unit is weighed by following quality：g)：Uniconazole P 0.5, Bachmedesh 1.5, acardite
0.5；Above-mentioned each composition is added in acetic acid (content >=75%) and supplies acetic acid to 100g, stirs, produces.
The preparation of composition II：Zasiokaurin stoste prepared by Preparative Example 1 is diluted with water 5 times, obtains zasiokaurin
Liquid.
By composition I and composition II according to 1:10000 mass ratio is well mixed, and is produced.
The preparation of the compound wheat seed soaking agent of embodiment 7
The preparation of composition I：Each composition (unit is weighed by following quality：g)：Uniconazole P 0.4, Bachmedesh 1.2, acardite
0.5；Above-mentioned each composition is added in acetic acid and supplies acetic acid (content >=75%) to 100g, stirs, produces.
The preparation of composition II：Zasiokaurin stoste prepared by Preparative Example 2 is diluted with water 8 times, obtains zasiokaurin
Liquid.
By composition I and composition II according to 1:7500 mass ratio is well mixed, and is produced.
The preparation of the compound wheat seed soaking agent of embodiment 8
The preparation of composition I：Each composition (unit is weighed by following quality：g)：Uniconazole P 0.5, Bachmedesh 1.5, acardite
0.6；Above-mentioned each composition is added in acetic acid and supplies acetic acid (content >=75%) to 100g, stirs, produces.
The preparation of composition II：Zasiokaurin stoste prepared by Preparative Example 3 is diluted with water 8 times, obtains zasiokaurin
Liquid.
By composition I and composition II according to 1:12500 mass ratio is well mixed, and is produced.
The screening test of the constituent of the compound wheat seed soaking agent of test example 1
First, test method
(1) the formula composition of compound seed soaking agent is respectively handled
The plant growth regulatings such as DCPTA, diethyl aminoethyl hexanoate, 6-BA, Bachmedesh, acardite are found according to primary screening test
Agent and zasiokaurin liquid have the effect of hestening rooting germination；This experiment is by uniconazole P and DCPTA, diethyl aminoethyl hexanoate, 6-BA, chrysanthemum second
The compositions such as amine ester, acardite, zasiokaurin liquid carry out compatibility in the way of table 1 and obtain the compound wheat seed soaking of different disposal
Agent.
Table 1 respectively handles the formula composition and preparation and extension rate of compound seed soaking agent
| Processing | The compound method of seed soaking agent | Extension rate | Dilution |
| 1 | Uniconazole P 0.5g, Bachmedesh 1.5g, acardite 0.5g, acetic acid supply 100g | 10000 | Zasiokaurin stoste dilutes 5 times |
| 2 | Uniconazole P 0.5g, DCPTA 1.5g, acardite 0.5g, acetic acid supply 100g | 10000 | Zasiokaurin stoste dilutes 5 times |
| 3 | Uniconazole P 0.5g, diethyl aminoethyl hexanoate 12g, acardite 0.5g, acetic acid supply 100g | 10000 | Zasiokaurin stoste dilutes 5 times |
| 4 | Uniconazole P 0.5g, Bachmedesh 1.5g, 6-BA0.5g, acetic acid supply 100g | 10000 | Zasiokaurin stoste dilutes 5 times |
| 5 | Uniconazole P 0.5g, DCPTA 1.5g, 6-BA0.5g, acetic acid supply 100g | 10000 | Zasiokaurin stoste dilutes 5 times |
| 6 | Uniconazole P 0.5g, diethyl aminoethyl hexanoate 12g, 6-BA0.5g, acetic acid supply 100g | 10000 | Zasiokaurin stoste dilutes 5 times |
| 7 | Blank (acetic acid) | 10000 | Zasiokaurin stoste dilutes 5 times |
| 8 | Uniconazole P 0.5g, Bachmedesh 1.5g, acardite 0.5g, acetic acid supply 100g | 10000 | Running water |
| 9 | Uniconazole P 0.5g, DCPTA 1.5g, acardite 0.5g, acetic acid supply 100g | 10000 | Running water |
| 10 | Uniconazole P 0.5g, diethyl aminoethyl hexanoate 12g, acardite 0.5g, acetic acid supply 100g | 10000 | Running water |
| 11 | Uniconazole P 0.5g, Bachmedesh 1.5g, 6-BA0.5g, acetic acid supply 100g | 10000 | Running water |
| 12 | Uniconazole P 0.5g, DCPTA 1.5g, 6-BA0.5g, acetic acid supply 100g | 10000 | Running water |
| 13 | Uniconazole P 0.5g, diethyl aminoethyl hexanoate 12g, 6-BA0.5g, acetic acid supply 100g | 10000 | Running water |
| 14 | Blank (acetic acid) | 10000 | Running water |
| 15 | Uniconazole P 3g, indolebutyric acid 30g, white vitriol 534g | 5000 | Running water |
(2) sand culture test
Sand culture test is carried out from the wheat seed of the kind of capital winter 17.Be 0.1mm-0.5mm from particle diameter, water content be
60%-80% quartz sand is matrix, and a height of 12cm × 12cm of length and width × 6cm transparent plastic germination box with cover is culture vessel
Carry out germination test.Experiment handles (being shown in Table 1) for totally 15, and acetic acid is blank control, and every kind of seed soaking agent carries out unified seed soaking
Processing (seed disinfection, after cleaning, after the seed soaking agent that respectively handles converts dilution by extension rate, per 3kg decoctions leaching 1kg seeds, 20
DEG C -30 DEG C are soaked seed 24 hours, and net decoction sowing is filtered after seed soaking), 4 repetitions, random district's groups arrangement.50 are sowed per box, in temperature
25 DEG C -30 DEG C, humidity 40%-60% of degree, light:Secretly=7:Cultivated under conditions of 5, from the 2d that sprouts, poured daily originally
Water 10ml/ boxes.After planting 7d takes 25 plants of seedling with dichotomy, and measurement plant height, stem foot are wide, root long, root bar number.
(3) Drought resistant experiment
Drought resistant experiment is carried out from the wheat seed of the kind of capital winter 17.Culture vessel be diameter 10cm culture dish, matrix
The water content of soil is the 15% of saturation moisture content, and each culture dish broadcasts 50 seeds.Experiment handles (being shown in Table 1) for totally 15, second
Acid is blank control, and every kind of seed soaking agent carries out unified seed soaking (same to sand culture test), 4 repetitions, random district's groups arrangement.
In 25 DEG C -30 DEG C, humidity 20%-40% of temperature, light:Secretly=7:Cultivated under conditions of 5, after planting 10d dichotomies
25 plants of seedling are taken, measure root long.
(4) Salt tolerance
Salt tolerance is carried out from the wheat seed of the kind of capital winter 17.Experiment handles (being shown in Table 1) for totally 15, and acetic acid is blank
Control, every kind of seed soaking agent carry out unified seed soaking (same to sand culture test).Each culture dish (diameter 10cm) spreads 3 layers of water suction
Paper, 1mol/LNaCl aqueous solution 10ml are poured into, be put into 50 selected, the seed for sprouting consistent, 4 repetitions, random district's groups row
Row.In 25 DEG C -30 DEG C, humidity 40%-60% of temperature, light:Secretly=7:Cultivated under conditions of 5, after planting 7d is with two points
Method takes 25 plants of seedling, measures root long.
(5) Insect resistance assay
Insect resistance assay is carried out from the wheat seed of the kind of capital winter 17, was entered in 2013 in Tongzhou District, Beijing City Huang Changpu villages
OK, preceding crop is corn, and experiment handles (being shown in Table 1) for totally 15, and acetic acid is blank control, and application rate is 12.5kg/ mus.At random
District's groups arrange, 4 repetitions, per plot area 30m2, protection row 1m is set between Qu Yuqu.Sowing is taken diagonally after 45 days per cell
Line is pinpointed, 3 points of samplings, and every is looked into 1m rows length, investigates basic seedling amount and killed seedling number, by subterranean pest-insect (such as cutworm, grub,
Wireworm, mole cricket etc.) seedling that bites or kill is killed seedling, calculate percentage of injury (percentage of injury=killed seedling number/basic seedling amount ×
100%).
2nd, result of the test
Sand culture test the results are shown in Table 2- tables 6, and resistance experiment (drought resisting, salt tolerant, pest-resistant) the results are shown in Table 7- tables 10.
Result of the test shows, by the seed soaking agent (processing after uniconazole P and Bachmedesh, acardite and zasiokaurin liquid compatibility
1) the wide increase by 167.9% of 44.5%, stem foot, root long increase, are reduced compared to control (processing 14), the plant height of wheat seedling
7.4%th, root bar number increase by 10.8%, insect resistace increase (percentage of injury reduces by 93.3%), drought resistance enhancing (root long increase
148.3%), salt tolerance enhancing (root long increase by 157.7%).Suppression of the uniconazole P to root can by Bachmedesh, acardite and
Zasiokaurin liquid is alleviated, and the suppression to plant height is not failed；It is visible according to result of the test, by uniconazole P and Bachmedesh, hexichol
Compound seed soaking agent collocation zasiokaurin liquid after urea compatibility uses, and is reducing plant height, promoting root growth promotees to strengthen, and increases lodging resistance and resistance
Etc. significant effect form (handling 2-15, difference reach the pole level of signifiance) better than other compoundings, this illustrates, alkene is imitated
Azoles has extremely significant Synergistic effect with Bachmedesh, acardite and zasiokaurin liquid phase compatibility.
The sand culture test result of table 2
| Processing | Plant height (cm) | Stem foot is wide (mm) | Root long (cm) | Root bar number |
| 1 | 5.21±0.1Ff | 3.49±0.13Aa | 11.4±0.36Aa | 5.02±0.18Aa |
| 2 | 6.38±0.11Ee | 2.33±0.07BCb | 10.43±0.41BCbcd | 4.29±0.09CDcd |
| 3 | 6.45±0.14Ee | 2.36±0.07Bb | 10.12±0.35Cd | 4.16±0.14DEd |
| 4 | 6.46±0.16Ee | 2.31±0.06BCbc | 10.15±0.28Cd | 4.23±0.13Dd |
| 5 | 8.74±0.31Cc | 1.77±0.07Gf | 7.83±0.23Ef | 3.63±0.14Gf |
| 6 | 8.68±0.25Cc | 1.74±0.07Gf | 8.16±0.28Ef | 3.65±0.05Gf |
| 7 | 10.89±0.27Aa | 2.32±0.06BCb | 10.73±0.35Bb | 4.69±0.08Bb |
| 8 | 6.32±0.17Ee | 2±0.03EFe | 10.24±0.26BCcd | 4.67±0.13CDd |
| 9 | 7.44±0.19Dd | 2.14±0.08Dd | 9.19±0.33De | 3.87±0.1Fe |
| 10 | 7.66±0.27Dd | 2.21±0.05CDcd | 9.2±0.24De | 3.95±0.06EFe |
| 11 | 7.69±0.31Dd | 2.14±0.07DEd | 9.04±0.2De | 3.88±0.11Fe |
| 12 | 8.77±0.28Cc | 1.78±0.04Gf | 8±0.17Ef | 3.59±0.12Gf |
| 13 | 8.7±0.09Cc | 1.75±0.07Gf | 8.09±0.23Ef | 3.59±0.12Gf |
| 14 | 9.75±0.19Bb | 1.97±0.06Fe | 10.61±0.42BCbc | 4.44±0.1Cc |
| 15 | 8.75±0.29Cc | 1.77±0.07Gf | 8.08±0.26Ef | 3.52±0.06Gf |
The sand culture test plant height analysis of variance table of table 3
| Source of variation | Quadratic sum | The free degree | It is square | F values | The level of signifiance |
| Between district's groups | 0.0943 | 3 | 0.0314 | 0.618 | 0.607 |
| Between processing | 129.3859 | 14 | 9.2418 | 181.756 | 0 |
| Error | 2.1356 | 42 | 0.0508 | ||
| Total variation | 131.6158 | 59 |
The wide analysis of variance table of sand culture test stem foot of table 4
| Source of variation | Quadratic sum | The free degree | It is square | F values | The level of signifiance |
| Between district's groups | 0.001 | 3 | 0.0003 | 0.066 | 0.9778 |
| Between processing | 10.9197 | 14 | 0.78 | 149.774 | 0 |
| Error | 0.2187 | 42 | 0.0052 | ||
| Total variation | 11.1394 | 59 |
The sand culture test root long analysis of variance table of table 5
| Source of variation | Quadratic sum | The free degree | It is square | F values | The level of signifiance |
| Between district's groups | 0.7875 | 3 | 0.2625 | 3.373 | 0.0271 |
| Between processing | 78.8251 | 14 | 5.6304 | 72.347 | 0 |
| Error | 3.2686 | 42 | 0.0778 | ||
| Total variation | 82.8812 | 59 |
The sand culture test root bar number analysis of variance table of table 6
| Source of variation | Quadratic sum | The free degree | It is square | F values | The level of signifiance |
| Between district's groups | 0.042 | 3 | 0.014 | 1.122 | 0.351 |
| Between processing | 12.3475 | 14 | 0.882 | 70.672 | 0 |
| Error | 0.5241 | 42 | 0.0125 | ||
| Total variation | 12.9136 | 59 |
The drought resisting of table 7, salt tolerant, Insect resistance assay result
| Processing | Drought resistant experiment root long (cm) | Salt tolerance root long (cm) | Percentage of injury (%) |
| 1 | 6.49±0.19Aa | 7.9±0.18Aa | 0.5±0.01Hh |
| 2 | 5.52±0.15Dcd | 7.04±0.31Bb | 2.84±0.1Cc |
| 3 | 5.59±0.12CDc | 7±0.25Bb | 2.84±0.04Cc |
| 4 | 5.81±0.04BCb | 6.99±0.14Bb | 2.74±0.1Cc |
| 5 | 5.2±0.15Ee | 6.3±0.1Cc | 1.99±0.06Dd |
| 6 | 5.35±0.12DEde | 6.35±0.09Cc | 2±0.05Dd |
| 7 | 4.5±0.06Hh | 4.84±0.09Ff | 11.71±0.21Aa |
| 8 | 5.95±0.09Bb | 7.47±0.29Bb | 0.88±0.02Gg |
| 9 | 4.92±0.15Ff | 5.91±0.06Dd | 1.13±0.02Ff |
| 10 | 4.92±0.08Ff | 5.84±0.16Dd | 1.11±0.02Ff |
| 11 | 4.88±0.08FGf | 5.92±0.12Dd | 1.12±0.02Ff |
| 12 | 4.68±0.18GHg | 5.42±0.03Ee | 1.66±0.02Ee |
| 13 | 4.68±0.13GHgh | 5.41±0.16Ee | 1.6±0.05Ee |
| 14 | 4.24±0.1Ii | 4.83±0.09Ff | 8.97±0.36Bb |
| 15 | 4.68±0.17FGHg | 5.4±0.21Ee | 1.6±0.05Ee |
The Drought resistant experiment root long analysis of variance table of table 8
| Source of variation | Quadratic sum | The free degree | It is square | F values | The level of signifiance |
| Between district's groups | 0.0635 | 3 | 0.0212 | 1.328 | 0.278 |
| Between processing | 21.3899 | 14 | 1.5278 | 95.815 | 0 |
| Error | 0.6697 | 42 | 0.0159 | ||
| Total variation | 22.1231 | 59 |
The Salt tolerance root long analysis of variance table of table 9
| Source of variation | Quadratic sum | The free degree | It is square | F values | The level of signifiance |
| Between district's groups | 0.2612 | 3 | 0.0871 | 3.494 | 0.0237 |
| Between processing | 49.5783 | 14 | 3.5413 | 142.123 | 0 |
| Error | 1.0465 | 42 | 0.0249 | ||
| Total variation | 50.886 | 59 |
The Insect resistance assay percentage of injury analysis of variance table of table 10
| Source of variation | Quadratic sum | The free degree | It is square | F values | The level of signifiance |
| Between district's groups | 0.0551 | 3 | 0.0184 | 1.382 | 0.2615 |
| Between processing | 561.4419 | 14 | 40.103 | 3018.9 | 0 |
| Error | 0.5579 | 42 | 0.0133 | ||
| Total variation | 562.0549 | 59 |
The inspection test of the constituent of the compound wheat seed soaking agent of test example 2
First, test method
(1) the formula composition of compound seed soaking agent is respectively handled
The result of the constituent screening test of test example 1 shows, after Bachmedesh, acardite and zasiokaurin liquid compounding
Inhibitory action of the uniconazole P to root system can be eliminated, other compound schemes are without similar effect.In view of plant growth regulator
Between influence it is extremely complex, it is understood that there may be a variety of influences such as synergy, antagonism, it is understood that there may be binary built is better than ternary built
Situation.In addition, from the aspect of cost and preparation processing, reducing medicament kind also has obvious advantage, so according to examination
Situation design binary built and the ternary built experiment of example 1 are tested, is designed with reference to the dosage of table 11.
Table 11 respectively handles the formula composition and preparation and extension rate of compound seed soaking agent
| Processing | The compound method of seed soaking agent | Extension rate | Dilution |
| 1 | Uniconazole P 0.5g, Bachmedesh 1.5g, acardite 0.5g, acetic acid supply 100g | 10000 | Zasiokaurin stoste dilutes 5 times |
| 2 | Uniconazole P 0.5g, Bachmedesh 1.5g, acetic acid supply 100g | 10000 | Zasiokaurin stoste dilutes 5 times |
| 3 | Uniconazole P 0.5g, DCPTA 1.5g, acetic acid supply 100g | 10000 | Zasiokaurin stoste dilutes 5 times |
| 4 | Uniconazole P 0.5g, diethyl aminoethyl hexanoate 12g, acetic acid supply 100g | 10000 | Zasiokaurin stoste dilutes 5 times |
| 5 | Uniconazole P 0.5g, acardite 0.5g, acetic acid supply 100g | 10000 | Zasiokaurin stoste dilutes 5 times |
| 6 | Uniconazole P 0.5g, 6-BA0.5g, acetic acid supply 100g | 10000 | Zasiokaurin stoste dilutes 5 times |
| 7 | Uniconazole P 0.5g, acetic acid supply 100g | 10000 | Zasiokaurin stoste dilutes 5 times |
| 8 | Blank (acetic acid) | 10000 | Zasiokaurin stoste dilutes 5 times |
(2) sand culture test
For specific method with test example 1, measurement index is root long.
(3) Drought resistant experiment
Specific method is the same as test example 1.
(4) Salt tolerance
Specific method is the same as test example 1.
(5) Insect resistance assay
Specific method is the same as test example 1.
2nd, result of the test
Result of the test is shown in Table 12- tables 16.
Visible according to result of the test, the significant effect that uniconazole P, Bachmedesh, acardite and pollen liquid compound is better than other
Compound scheme (handling 2-8, difference reaches the pole level of signifiance), binding tests example 1 considers, uniconazole P, Bachmedesh and
The compounding effect of acardite will be substantially better than other compound schemes, and this shows the three plant growths regulation in the compound scheme
There is obvious Synergistic effect between agent and zasiokaurin liquid.
The constituent inspection test result of table 12
| Processing | Sand culture test root long (cm) | Drought resistant experiment root long (cm) | Salt tolerance root long (cm) | Percentage of injury (%) |
| 1 | 12.2±0.28Aa | 6.76±0.24Aa | 7.35±0.1Aa | 0.69±0.02Ee |
| 2 | 8.81±0.28CDc | 5.84±0.2BCbc | 6.15±0.13BCbc | 1.74±0.04Dd |
| 3 | 8.74±0.05CDc | 5.63±0.17Ccd | 6.01±0.15BCcd | 2.22±0.07Cc |
| 4 | 8.77±0.26CDc | 5.98±0.24Bb | 6.17±0.27BCbc | 1.73±0.07Dd |
| 5 | 8.92±0.37Cc | 5.8±0.1BCbc | 6.25±0.13Bb | 1.71±0.05Dd |
| 6 | 8.29±0.34Dd | 5.56±0.17Cd | 5.89±0.12Cd | 2.17±0.09Cc |
| 7 | 6.32±0.11Ee | 4.82±0.18De | 5.34±0.13De | 6.07±0.27Bb |
| 8 | 11±0.32Bb | 4.69±0.12De | 4.98±0.17Ef | 8.34±0.2Aa |
The sand culture test root long analysis of variance table of table 13
| Source of variation | Quadratic sum | The free degree | It is square | F values | The level of signifiance |
| Between district's groups | 0.0987 | 3 | 0.0329 | 0.414 | 0.7445 |
| Between processing | 87.8425 | 7 | 12.5489 | 158.079 | 0 |
| Error | 1.6671 | 21 | 0.0794 | ||
| Total variation | 89.6083 | 31 |
The Drought resistant experiment root long analysis of variance table of table 14
The Salt tolerance root long analysis of variance table of table 15
| Source of variation | Quadratic sum | The free degree | It is square | F values | The level of signifiance |
| Between district's groups | 0.0366 | 3 | 0.0122 | 0.455 | 0.7165 |
| Between processing | 13.7669 | 7 | 1.9667 | 73.434 | 0 |
| Error | 0.5624 | 21 | 0.0268 | ||
| Total variation | 14.3658 | 31 |
The Insect resistance assay percentage of injury analysis of variance table of table 16
| Source of variation | Quadratic sum | The free degree | It is square | F values | The level of signifiance |
| Between district's groups | 0.0221 | 3 | 0.0074 | 0.415 | 0.7438 |
| Between processing | 197.6435 | 7 | 28.2348 | 1593.791 | 0 |
| Error | 0.372 | 21 | 0.0177 | ||
| Total variation | 198.0376 | 31 |
The dosage screening test of the constituent of the compound wheat seed soaking agent of test example 3
First, test method
(1) the dosage design of the constituent of compound wheat seed soaking agent
According to dosage 15 treatment groups of design of table 17.
Table 17 respectively handles the compound method of seed soaking agent
| Processing | The compound method of seed soaking agent | Extension rate | Dilution |
| 1 | Uniconazole P 0.5g, Bachmedesh 1.5g, acardite 0.5g, acetic acid supply 100g | 10000 | Zasiokaurin stoste dilutes 5 times |
| 2 | Uniconazole P 0.3g, Bachmedesh 1.5g, acardite 0.5g, acetic acid supply 100g | 10000 | Zasiokaurin stoste dilutes 5 times |
| 3 | Uniconazole P 0.4g, Bachmedesh 1.5g, acardite 0.5g, acetic acid supply 100g | 10000 | Zasiokaurin stoste dilutes 5 times |
| 4 | Uniconazole P 0.6g, Bachmedesh 1.5g, acardite 0.5g, acetic acid supply 100g | 10000 | Zasiokaurin stoste dilutes 5 times |
| 5 | Uniconazole P 0.5g, Bachmedesh 0.9g, acardite 0.5g, acetic acid supply 100g | 10000 | Zasiokaurin stoste dilutes 5 times |
| 6 | Uniconazole P 0.5g, Bachmedesh 1.2g, acardite 0.5g, acetic acid supply 100g | 10000 | Zasiokaurin stoste dilutes 5 times |
| 7 | Uniconazole P 0.5g, Bachmedesh 1.8g, acardite 0.5g, acetic acid supply 100g | 10000 | Zasiokaurin stoste dilutes 5 times |
| 8 | Uniconazole P 0.5g, Bachmedesh 1.5g, acardite 0.4g, acetic acid supply 100g | 10000 | Zasiokaurin stoste dilutes 5 times |
| 9 | Uniconazole P 0.5g, Bachmedesh 1.5g, acardite 0.6g, acetic acid supply 100g | 10000 | Zasiokaurin stoste dilutes 5 times |
| 10 | Uniconazole P 0.5g, Bachmedesh 1.5g, acardite 0.7g, acetic acid supply 100g | 10000 | Zasiokaurin stoste dilutes 5 times |
| 11 | Uniconazole P 0.5g, Bachmedesh 1.5g, acardite 0.5g, acetic acid supply 100g | 10000 | The 1 times of dilution of zasiokaurin stoste |
| 12 | Uniconazole P 0.5g, Bachmedesh 1.5g, acardite 0.5g, acetic acid supply 100g | 10000 | The 2 times of dilutions of zasiokaurin stoste |
| 13 | Uniconazole P 0.5g, Bachmedesh 1.5g, acardite 0.5g, acetic acid supply 100g | 10000 | The 8 times of dilutions of zasiokaurin stoste |
| 14 | Uniconazole P 0.5g, Bachmedesh 1.5g, acardite 0.5g, acetic acid supply 100g | 10000 | The 9 times of dilutions of zasiokaurin stoste |
| 15 | Uniconazole P 0.6g, Bachmedesh 0.9g, acardite 0.7g, acetic acid supply 100g | 10000 | Zasiokaurin stoste dilutes 5 times |
(2) sand culture test
For specific method with test example 1, measurement index is root long.
(3) Drought resistant experiment
Specific method is the same as test example 1.
(4) Salt tolerance
Specific method is the same as test example 1.
(5) Insect resistance assay
Specific method is the same as test example 1.
2nd, result of the test
Result of the test is shown in Table 18- tables 22.
Visible according to result of the test, the preferable compound scheme of effect is：Uniconazole P 0.4%-0.5%+ Bachmedeshs
1.2%-1.5%+ acardite 0.5%-0.6% collocation zasiokaurins liquid (zasiokaurin stoste dilutes 5 times), the compound scheme exists
Promote root growth, resistance significant effect better than other schemes outside the compound scheme scope (difference reaches the level of signifiance)；Its
In, the collocation zasiokaurin of uniconazole P 0.5%+ Bachmedesh 1.5%+ acardite 0.5% liquid (zasiokaurin stoste dilutes 5 times)
Effect is best in compound scheme, the other formulas being significantly better than in the compound scheme (difference reaches the pole level of signifiance).
The constituent dosage result of the test of table 18
| Processing | Sand culture test root long (cm) | Drought resistant experiment root long (cm) | Salt tolerance root long (cm) | Percentage of injury (%) |
| 1 | 10.95±0.2Aa | 7.86±0.21Aa | 6.94±0.05Aa | 0.79±0.03Ee |
| 2 | 7.71±0.21Cc | 6.06±0.25DEFde | 5.75±0.18DEFGHfgh | 1.74±0.06Cc |
| 3 | 8.31±0.24Bb | 6.72±0.23BCb | 5.93±0.1BCDEFcdef | 1.4±0.05Dd |
| 4 | 6.78±0.21Dd | 6.11±0.18DEFde | 5.52±0.08GHij | 1.71±0.06Cc |
| 5 | 7.51±0.21Cc | 6.17±0.13DEFcd | 5.8±0.09CDEFGdefg | 1.71±0.04Cc |
| 6 | 8.57±0.19Bb | 6.82±0.22Bb | 6.04±0.16BCbc | 1.39±0.03Dd |
| 7 | 7.6±0.37Cc | 6.02±0.13EFde | 5.71±0.19EFGHghi | 1.7±0.04Cc |
| 8 | 7.41±0.15Cc | 6.4±0.05CDc | 5.79±0.18CDEFGefg | 1.72±0.03Cc |
| 9 | 8.38±0.29Bb | 6.82±0.12Bb | 5.96±0.06BCDEbcde | 1.38±0.03Dd |
| 10 | 7.55±0.19Cc | 6.11±0.24DEFde | 5.54±0.08GHhij | 1.72±0.04Cc |
| 11 | 7.54±0.23Cc | 6.13±0.27DEFcde | 5.67±0.24FGHghij | 1.69±0.04Cc |
| 12 | 8.38±0.24Bb | 6.83±0.29Bb | 6.01±0.09BCDbcd | 1.39±0.05Dd |
| 13 | 8.3±0.26Bb | 6.96±0.07Bb | 6.14±0.2Bb | 1.44±0.01Dd |
| 14 | 7.61±0.22Cc | 6.23±0.27DEcd | 5.7±0.03EFGHghij | 2±0.06Bb |
| 15 | 6.19±0.2Ee | 5.85±0.27Fe | 5.5±0.19Hj | 2.23±0.07Aa |
The sand culture test root long analysis of variance table of table 19
| Source of variation | Quadratic sum | The free degree | It is square | F values | The level of signifiance |
| Between district's groups | 0.1442 | 3 | 0.0481 | 0.885 | 0.4567 |
| Between processing | 62.3021 | 14 | 4.4501 | 81.924 | 0 |
| Error | 2.2815 | 42 | 0.0543 | ||
| Total variation | 64.7278 | 59 |
The Drought resistant experiment root long analysis of variance table of table 20
| Source of variation | Quadratic sum | The free degree | It is square | F values | The level of signifiance |
| Between district's groups | 0.2827 | 3 | 0.0942 | 2.379 | 0.0833 |
| Between processing | 15.5996 | 14 | 1.1143 | 28.129 | 0 |
| Error | 1.6637 | 42 | 0.0396 | ||
| Total variation | 17.546 | 59 |
The Salt tolerance root long analysis of variance table of table 21
| Source of variation | Quadratic sum | The free degree | It is square | F values | The level of signifiance |
| Between district's groups | 0.0461 | 3 | 0.0154 | 0.72 | 0.5457 |
| Between processing | 7.0375 | 14 | 0.5027 | 23.53 | 0 |
| Error | 0.8973 | 42 | 0.0214 | ||
| Total variation | 7.9809 | 59 |
The Insect resistance assay percentage of injury analysis of variance table of table 22
| Source of variation | Quadratic sum | The free degree | It is square | F values | The level of signifiance |
| Between district's groups | 0.0042 | 3 | 0.0014 | 0.658 | 0.5825 |
| Between processing | 6.0204 | 14 | 0.43 | 203.17 | 0 |
| Error | 0.0889 | 42 | 0.0021 | ||
| Total variation | 6.1135 | 59 |
Claims (20)
1. a kind of compound wheat seed soaking agent, it is characterised in that be made up of following composition：Uniconazole P, Bachmedesh, acardite and
Acetic acid；By mass percentage, the dosage of each composition is：Uniconazole P 0.4%-0.5%, Bachmedesh 1.2%-1.5%, hexichol
Urea 0.5%-0.6%, surplus are acetic acid.
2. according to the compound wheat seed soaking agent described in claim 1, it is characterised in that：The dosage of each composition is：Uniconazole P
0.5%th, Bachmedesh 1.5%, acardite 0.5%.
3. according to the compound wheat seed soaking agent described in claim 1, it is characterised in that：Described acetic acid is the second of content >=75%
Acid.
A kind of 4. method for preparing compound wheat seed soaking agent described in claim 1, it is characterised in that this method includes：Alkene is imitated
Azoles, Bachmedesh and acardite are added in acetic acid, are stirred, and are produced.
A kind of 5. compound wheat seed soaking agent, it is characterised in that：It is made up of composition I and composition II；Wherein, the composition I is imitated by alkene
Azoles, Bachmedesh, acardite and acetic acid composition, by mass percentage, the dosage of each composition is in composition I：Uniconazole P
0.4%-0.5%, Bachmedesh 1.2%-1.5%, acardite 0.5%-0.6%, surplus are acetic acid；The composition II is corn
Pollen liquid.
6. according to the compound wheat seed soaking agent described in claim 5, it is characterised in that：Described acetic acid is the second of content >=75%
Acid.
7. according to the compound wheat seed soaking agent described in claim 5, it is characterised in that：The dosage of I each composition of composition is：Uniconazole P
0.5%th, Bachmedesh 1.5%, acardite 0.5%；According to mass ratio meter, composition I:Composition II=1:5000-15000.
8. according to the compound wheat seed soaking agent described in claim 7, it is characterised in that：Composition I:Composition II=1:7500-
12500。
9. according to the compound wheat seed soaking agent described in claim 8, it is characterised in that：Composition I:Composition II=1:10000.
10. according to the compound wheat seed soaking agent described in claim 5, it is characterised in that：Described zasiokaurin liquid is by corn
Pollen stoste obtains after being diluted with water；
The zasiokaurin stoste is prepared in accordance with the following methods：
(1) zasiokaurin is gathered；
(2) zasiokaurin is freezed to storage at a temperature of -10 DEG C~-20 DEG C；
(3) zasiokaurin freezed after depositing is added in hot water and stirred, cooled down, produce.
11. according to the compound wheat seed soaking agent described in claim 10, it is characterised in that：Described zasiokaurin liquid is by corn
Pollen stoste obtains after being diluted with water 2-20 times.
12. according to the compound wheat seed soaking agent described in claim 11, it is characterised in that：Described zasiokaurin liquid is by corn
Pollen stoste obtains after being diluted with water 2-8 times.
13. according to the compound wheat seed soaking agent described in claim 12, it is characterised in that：Described zasiokaurin liquid is by corn
Pollen stoste obtains after being diluted with water 5 times.
14. according to the compound wheat seed soaking agent described in claim 10, it is characterised in that：
The zasiokaurin stoste is prepared in accordance with the following methods：
(1) zasiokaurin is gathered in corn full-bloom stage, the zasiokaurin that the same day gathers is exposed to the sun 1-2 hours in the sun；
(2) zasiokaurin after being exposed to the sun is freezed to storage at a temperature of -20 DEG C；
(3) zasiokaurin for freezing storage at least 30 days is added in 80-100 DEG C of hot water of 3-20 times of quality and stirred,
Produced after cooling.
15. according to the compound wheat seed soaking agent described in claim 14, it is characterised in that：In step (3) by freezing storage at least
The zasiokaurin of 30 days is added in 100 DEG C of hot water of 10 times of quality.
A kind of 16. method for preparing the compound wheat seed soaking agent described in claim 5, it is characterised in that including：By the He of composition I
Composition II is well mixed, produces.
17. compound wheat seed soaking agent described in claim 1-3 any one reduce Plant Height in Wheat, promoting root growth promote strengthen, increase it is anti-fall
Property or improve resistance in terms of in application.
18. according to the application described in claim 17, it is characterised in that the application includes：By the compound wheat seed soaking agent
It is diluted with water, the compound wheat seed soaking agent after being diluted；Wheat seed is soaked with the compound wheat seed soaking agent after dilution
Kind；Wherein, according to mass ratio meter, the ratio of wheat seed and the compound wheat seed soaking agent liquid after dilution is 1:3；Described leaching
Bubble is soaked seed 24 hours at a temperature of 20 DEG C -30 DEG C.
19. compound wheat seed soaking agent described in claim 5-15 any one reduce Plant Height in Wheat, promoting root growth promote strengthen, increase it is anti-fall
Property or improve resistance in terms of in application.
20. according to the application described in claim 19, it is characterised in that the application includes：Wheat seed is used into described answer
Wheat seed soaking agent is closed to be soaked seed；Wherein, according to mass ratio meter, the ratio of wheat seed and compound wheat seed soaking agent is 1:3；
Described immersion is soaked seed 24 hours at a temperature of 20 DEG C -30 DEG C.
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| CN101194610A (en) * | 2007-12-26 | 2008-06-11 | 中国科学院东北地理与农业生态研究所 | Method for preventing and treating bald tip of corn |
| CN101642132B (en) * | 2009-08-27 | 2012-01-25 | 华南农业大学 | Cold-resistant seed soaking agent for direct sowing of rice as well as preparation method and applications thereof |
| CN101774852B (en) * | 2010-01-12 | 2013-01-16 | 湖南农大海特农化有限公司 | Multifunctional composite seed coating agent |
| CN102365957B (en) * | 2011-09-13 | 2016-06-29 | 辽宁师范大学 | A kind of Uniconazole microemulsion, preparation method and application |
| CN103299911B (en) * | 2013-07-16 | 2015-02-18 | 葛建 | Method for obtaining virus-free seedlings of pure cymbidium efficiently |
| CN104025754B (en) * | 2014-05-16 | 2015-05-20 | 和县绿丰种业有限公司 | Wuyueman seed germination accelerating method |
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