CN106818873A - A kind of preparation method for improving wheat frost resistance spacetabs type seed soak - Google Patents
A kind of preparation method for improving wheat frost resistance spacetabs type seed soak Download PDFInfo
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- CN106818873A CN106818873A CN201611221168.0A CN201611221168A CN106818873A CN 106818873 A CN106818873 A CN 106818873A CN 201611221168 A CN201611221168 A CN 201611221168A CN 106818873 A CN106818873 A CN 106818873A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/26—Phosphorus; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/06—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing keto or thioketo groups as part of a ring, e.g. cyclohexanone, quinone; Derivatives thereof, e.g. ketals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/06—Aluminium; Calcium; Magnesium; Compounds thereof
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Pest Control & Pesticides (AREA)
- Inorganic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Soil Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a kind of preparation method for improving wheat frost resistance spacetabs type seed soak, belong to the technical field of antifreeze.Raising wheat frost resistance spacetabs type seed soak prepared by the present invention is during wheat seed is soaked, parcel wheat seed, slow release amino acid and calcium, wheat is promoted to take root, while wheat seed absorbing irritants, excite the increase of wheat seed inside superoxide dismutase and freeze proof enzyme matrix, lure that wheat improves frost resistance in the starting stage into, the content of chlorophyll is improved simultaneously, so as to efficiently solve carry out covering to wheat and easily cause wheat during tradition is antifreeze to wheat at present that enough sunlight can not be obtained, photosynthesis cannot be carried out, cause Wheat Development bad, harsh environment is needed to require wheat winter irrigation simultaneously, and have resulted in frozen soil, the problem damaged to wheat.The present invention improves the content of wheat chlorophyll and Proline, increased SOD activity, and enhancing root system activity improves the cold tolerance of wheat.
Description
Technical field
The invention discloses a kind of preparation method for improving wheat frost resistance spacetabs type seed soak, belong to antifreeze
Technical field.
Background technology
Low Temperature in Spring freeze injury is one of major weather disaster of China Yellow River-Huai River region and middle and lower reach of Yangtze River area of wheat Wheat Production,
It occurs frequently, occurring area is big, harm weight, has a strong impact on and govern Wheat Production.With global warming, China goes out
The phenomenon of existing winter warm cold spell in spring is frequent, so as to cause wheat Spring cold injury constantly to occur." cold spell in later spring " is common Low Temperature in Spring
Freeze injury, it is multiple to be born in mid-March to mid or late April, because now Differentiation of Wheat Ears is to the tetrad phase, its to cryogenic freezing most
It is sensitivity, freeze injury easily occurs.Previous karyotype studies show, wheat by freeze injury degree not only with kind, cooling intensity and low temperature
Duration length is relevant, also has much relations with the aspect such as date of seeding, the amount of broadcasting, soil and water and fertilizer management measure.
Traditional method for preventing wheat freeze proof has two kinds:1st, covering is increased, it is ensured that weak seedling safe overwintering:(1)Covering straw
Stalk.Before winter in wheat in the ranks, wheat bran, broken wheat straw etc. are spread, there is soil moisture conservation, antifreeze effect, can foster and apply fertilizer ground with improved soil after rotting
Power, is wheatland drought resisting, antifreeze, volume increase effective measures.(2)Blinding miscellaneous fertilizers.After wheat enters Wintering Period, soil is spread fertilizer over the fields along ridge miscellaneous
Fertilizer, can be preserved soil moisture with wind sheltering, heat it is antifreeze, and for wheat seeding turn green growth supplement nutrient;2nd, in good time winter irrigation, prevents frost.The in good time winter
Fill, be the effective measures for preventing wheat winter freeze calamity, winter irrigation can form good soil moisture environment, the soil in regulation topsoil
Nutrient, improves the thermal capacity of soil, can typically improve 1~3 DEG C of ground temperature, while native seam can be made up, promotes many tillers, grows up
Tiller, educate strong sprout.Winter irrigation will be watered at 4 DEG C or so with daily mean temperature and is advisable, and had if less than this temperature winter irrigation and frozen
Harmful danger.Winter irrigation will also depending on soil property, the growth of cereal crop seedlings, soil moisture content and flexibly grasp, clay suitably should be filled early, sand ground should evening fill, rice stubble
Ground or the good wheatland of soil moisture can not be filled.
Current conventional method is antifreeze to being carried out after wheat growth, covering is carried out to wheat easily causes wheat to obtain
Enough sunlight, carries out photosynthesis, causes Wheat Development bad, needs harsh environment to require wheat winter irrigation, and have resulted in
Frozen soil, damages to wheat.
The content of the invention
The technical problems to be solved by the invention:For current tradition it is antifreeze to wheat during wheat cover easily
Causing wheat can not obtain enough sunlight, it is impossible to carry out photosynthesis, cause Wheat Development bad, while being needed to wheat winter irrigation
Harsh environment requirement, and frozen soil is had resulted in, the problem damaged to wheat.The present invention using chicken bone as protein source,
Abundant aminoacids solution is obtained by digesting, under sour environment and ethylenediamine effect, is combined with free calcium, form amino acid chela
Calcium is closed, then sodium alginate is used as tackifier, then adds hypericin etc. as stimulant, finally mixed with nutrients
Compound, so as to be obtained improve wheat frost resistance spacetabs type seed soak.Raising wheat frost resistance spacetabs type prepared by the present invention
Seed soak wraps up wheat seed during wheat seed is soaked, and slow release amino acid and calcium promote wheat to take root,
Wheat seed absorbing irritants, excite the increase of wheat seed inside superoxide dismutase and freeze proof enzyme matrix simultaneously, lure into
Wheat improves frost resistance in the starting stage, while the content of chlorophyll is improved, it is anti-to wheat so as to efficiently solve tradition at present
Covering is carried out to wheat during jelly easily cause wheat that enough sunlight can not be obtained, carry out photosynthesis, cause Wheat Development not
It is good, while needing harsh environment to require wheat winter irrigation, and frozen soil is had resulted in, the problem damaged to wheat.The present invention
The raising wheat frost resistance spacetabs type seed soak of gained significantly improves the content of wheat chlorophyll and Proline, increases
Add SOD activity, reduced MDA contents, enhanced root system activity, improve the cold tolerance of wheat.
In order to solve the above technical problems, the present invention is using technical scheme as described below:
(1)Take during chicken bone is put into 50 DEG C of drying boxes and dry 1~2h, dried chicken bone is put into pulverizer carries out powder
It is broken, 50 mesh sieves are crossed, collect sieving particle, in mass ratio 1:4:7~1:4:9, taking complex enzyme, sieving particle and mass fraction is
10% lactic acid solution is put into enzymatic vessel, and design temperature is 33~37 DEG C, with 6~8h of 130r/min stirring enzymolysis;It is described multiple
Synthase is trypsase and pepsin in mass ratio 1:1 mixes;
(2)After above-mentioned enzymolysis terminates, the enzymolysis mixture in enzymatic vessel is put into centrifuge, with 1200r/min centrifugations point
From 10~15min, supernatant is collected, counted by weight, it is 15% chlorine to take 60~65 portions of supernatants, 40~45 parts of mass fractions
Change calcium solution, 12~16 parts of glycine and 4~7 parts of ethylenediamines, be put into reactor, 80~85 DEG C of design temperature, with 150r/
2~3h of min stirring reactions;
(3)After above-mentioned reaction terminates, room temperature is naturally cooled to, discharging is collected out material, filtered to going out material, collected
Filtrate, by the sodium alginate of filtered fluid and filtered fluid quality 30~35% be put into mixer with 200r/min stirrings 10~
15min, adds the hypericin of sodium alginate quality 10~15%, continues to stir 40~45min, collects stirring mixture,
Obtain spacetabs type seed soak base fluid;
(4)Count by weight, take 45~50 parts of above-mentioned spacetabs type seed soak base fluids, 8~11 parts of distilled water, 5~7 parts of phosphorus
Sour hydrogen dipotassium and 1~3 part of 6-benzyl aminopurine, are put into glass reaction kettle, are protected using oxygen, with 160r/min stirring 1~
2h, collects out material, you can must improve wheat frost resistance spacetabs type seed soak.
Application process of the invention is:By wheat seed and the raising wheat frost resistance spacetabs type seed leaching obtained by the present invention
Bubble liquid in mass ratio 1:3~1:5 ratio is put into container and is stirred, and the temperature in control container is 3~5 DEG C, in sun
After placing 5~7h under light, after seed is dried naturally can sowing, after testing, the raising wheat frost resistance obtained by the present invention is slow
Chlorophyll content and root activity that type seed immersion liquid energy dramatically increases wheat are released, in the jointing stage, the chlorophyll of wheat contains
Amount increased 2~3mg/g, and the root activity of wheat increased 17~23%.
Compared with other method, Advantageous Effects are the present invention:
(1)Raising wheat frost resistance spacetabs type seed soak obtained by the present invention can increase the chlorophyll content of wheat, carry
The photosynthesis of wheat high, enhancing wheat resists the ability of freeze injury, and what reduction wheat root was subject to injures;
(2)Raising wheat frost resistance spacetabs type seed immersion formula of liquid obtained by the present invention is simple, easy to use, and will not be to small
Wheat produces injury.
Specific embodiment
Take during chicken bone is put into 50 DEG C of drying boxes and dry 1~2h, dried chicken bone is put into pulverizer carries out powder
It is broken, 50 mesh sieves are crossed, collect sieving particle, in mass ratio 1:4:7~1:4:9, taking complex enzyme, sieving particle and mass fraction is
10% lactic acid solution is put into enzymatic vessel, and design temperature is 33~37 DEG C, with 6~8h of 130r/min stirring enzymolysis;It is described multiple
Synthase is trypsase and pepsin in mass ratio 1:1 mixes;After above-mentioned enzymolysis terminates, by the enzymolysis in enzymatic vessel
Mixture is put into centrifuge, with 1200r/min 10~15min of centrifugation, collects supernatant, is counted by weight, takes 60
~65 parts of supernatants, 40~45 parts of mass fractions are 15% calcium chloride solution, 12~16 parts of glycine and 4~7 parts of ethylenediamines, are put
In entering reactor, 80~85 DEG C of design temperature, with 2~3h of 150r/min stirring reactions;After above-mentioned reaction terminates, natural cooling
To room temperature, material is collected out in discharging, is filtered to going out material, collects filtered fluid, by filtered fluid and filtered fluid quality 30~
35% sodium alginate is put into mixer and stirs 10~15min with 200r/min, adds sodium alginate quality 10~15%
Hypericin, continue stir 40~45min, collect stirring mixture, obtain spacetabs type seed soak base fluid;By weight
Meter, takes 45~50 parts of above-mentioned spacetabs type seed soak base fluids, 8~11 parts of distilled water, 5~7 parts of dipotassium hydrogen phosphates and 1~3 part
6-benzyl aminopurine, is put into glass reaction kettle, is protected using oxygen, and 1~2h is stirred with 160r/min, collects out material, i.e.,
Wheat frost resistance spacetabs type seed soak can be improved.
Example 1
Take during chicken bone is put into 50 DEG C of drying boxes and dry 1h, dried chicken bone is put into pulverizer and is crushed, cross 50
Mesh sieve, collects sieving particle, in mass ratio 1:4:7, take complex enzyme, sieving particle and lactic acid solution that mass fraction is 10% and put
Enter in enzymatic vessel, design temperature is 33 DEG C, with 130r/min stirring enzymolysis 6h;The complex enzyme is trypsase and pepsin
In mass ratio 1:1 mixes;After above-mentioned enzymolysis terminates, the enzymolysis mixture in enzymatic vessel is put into centrifuge, with
1200r/min centrifugation 10min, collect supernatant, count by weight, and taking 60 portions of supernatants, 40 parts of mass fractions is
15% calcium chloride solution, 12 parts of glycine and 4 parts of ethylenediamines, are put into reactor, 80 DEG C of design temperature, are stirred with 150r/min
Reaction 2h;After above-mentioned reaction terminates, room temperature is naturally cooled to, discharging is collected out material, filtered to going out material, collected
Filtrate, the sodium alginate of filtered fluid and filtered fluid quality 30% is put into mixer and stirs 10min with 200r/min, is added
The hypericin of sodium alginate quality 10%, continues to stir 40min, collects stirring mixture, obtains spacetabs type seed immersion liquid-based
Liquid;Count by weight, take 45 parts of above-mentioned spacetabs type seed soak base fluids, 8 parts of distilled water, 5 parts of dipotassium hydrogen phosphates and 1 part of 6-
Benayl aminopurine, is put into glass reaction kettle, is protected using oxygen, and 1h is stirred with 160r/min, collects out material, you can must carry
Wheat frost resistance spacetabs type seed soak high.
By wheat seed and the raising wheat frost resistance spacetabs type seed soak in mass ratio 1 obtained by the present invention:3 ratio
Example is put into container and is stirred, and the temperature in control container is 3 DEG C, after placing 5h in the sun, seed is dried naturally
Can sow afterwards, after testing, the raising wheat frost resistance spacetabs type seed immersion liquid energy obtained by the present invention dramatically increases wheat
Chlorophyll content and root activity, in the jointing stage, the chlorophyll content of wheat increased 2mg/g, and the root activity of wheat increases
Add 17%.
Example 2
Take during chicken bone is put into 50 DEG C of drying boxes and dry 2h, dried chicken bone is put into pulverizer and is crushed, cross 50
Mesh sieve, collects sieving particle, in mass ratio 1:4:9, take complex enzyme, sieving particle and lactic acid solution that mass fraction is 10% and put
Enter in enzymatic vessel, design temperature is 37 DEG C, with 130r/min stirring enzymolysis 8h;The complex enzyme is trypsase and pepsin
In mass ratio 1:1 mixes;After above-mentioned enzymolysis terminates, the enzymolysis mixture in enzymatic vessel is put into centrifuge, with
1200r/min centrifugation 15min, collect supernatant, count by weight, and taking 65 portions of supernatants, 45 parts of mass fractions is
15% calcium chloride solution, 16 parts of glycine and 7 parts of ethylenediamines, are put into reactor, 85 DEG C of design temperature, are stirred with 150r/min
Reaction 3h;After above-mentioned reaction terminates, room temperature is naturally cooled to, discharging is collected out material, filtered to going out material, collected
Filtrate, the sodium alginate of filtered fluid and filtered fluid quality 35% is put into mixer and stirs 15min with 200r/min, is added
The hypericin of sodium alginate quality 15%, continues to stir 45min, collects stirring mixture, obtains spacetabs type seed immersion liquid-based
Liquid;Count by weight, take 50 parts of above-mentioned spacetabs type seed soak base fluids, 11 parts of distilled water, 7 parts of dipotassium hydrogen phosphates and 3 parts
6-benzyl aminopurine, is put into glass reaction kettle, is protected using oxygen, and 2h is stirred with 160r/min, collects out material, you can
Improve wheat frost resistance spacetabs type seed soak.
By wheat seed and the raising wheat frost resistance spacetabs type seed soak in mass ratio 1 obtained by the present invention:5 ratio
Example is put into container and is stirred, and the temperature in control container is 5 DEG C, after placing 7h in the sun, seed is dried naturally
Can sow afterwards, after testing, the raising wheat frost resistance spacetabs type seed immersion liquid energy obtained by the present invention dramatically increases wheat
Chlorophyll content and root activity, in the jointing stage, the chlorophyll content of wheat increased 3mg/g, and the root activity of wheat increases
Add 23%.
Example 3
Take during chicken bone is put into 50 DEG C of drying boxes and dry 2h, dried chicken bone is put into pulverizer and is crushed, cross 50
Mesh sieve, collects sieving particle, in mass ratio 1:4:8, take complex enzyme, sieving particle and lactic acid solution that mass fraction is 10% and put
Enter in enzymatic vessel, design temperature is 35 DEG C, with 130r/min stirring enzymolysis 7h;The complex enzyme is trypsase and pepsin
In mass ratio 1:1 mixes;After above-mentioned enzymolysis terminates, the enzymolysis mixture in enzymatic vessel is put into centrifuge, with
1200r/min centrifugation 13min, collect supernatant, count by weight, and taking 63 portions of supernatants, 42 parts of mass fractions is
15% calcium chloride solution, 14 parts of glycine and 5 parts of ethylenediamines, are put into reactor, 82 DEG C of design temperature, are stirred with 150r/min
Reaction 2.5h;After above-mentioned reaction terminates, room temperature is naturally cooled to, discharging is collected out material, filtered to going out material, is collected
Filtered fluid, the sodium alginate of filtered fluid and filtered fluid quality 33% is put into mixer and stirs 12min with 200r/min, then added
Enter the hypericin of sodium alginate quality 13%, continue to stir 43min, collect stirring mixture, obtain spacetabs type seed soak
Base fluid;Count by weight, take 47 parts of above-mentioned spacetabs type seed soak base fluids, 10 parts of distilled water, 6 parts of dipotassium hydrogen phosphates and 2
Part 6-benzyl aminopurine, is put into glass reaction kettle, is protected using oxygen, and 1h is stirred with 160r/min, collects out material, you can
Wheat frost resistance spacetabs type seed soak must be improved.
By wheat seed and the raising wheat frost resistance spacetabs type seed soak in mass ratio 1 obtained by the present invention:4 ratio
Example is put into container and is stirred, and the temperature in control container is 4 DEG C, after placing 6h in the sun, seed is dried naturally
Can sow afterwards, after testing, the raising wheat frost resistance spacetabs type seed immersion liquid energy obtained by the present invention dramatically increases wheat
Chlorophyll content and root activity, in the jointing stage, the chlorophyll content of wheat increased 3mg/g, and the root activity of wheat increases
Add 20%.
Claims (1)
1. a kind of preparation method for improving wheat frost resistance spacetabs type seed soak, it is characterised in that specific preparation process is:
(1)Take during chicken bone is put into 50 DEG C of drying boxes and dry 1~2h, dried chicken bone is put into pulverizer carries out powder
It is broken, 50 mesh sieves are crossed, collect sieving particle, in mass ratio 1:4:7~1:4:9, taking complex enzyme, sieving particle and mass fraction is
10% lactic acid solution is put into enzymatic vessel, and design temperature is 33~37 DEG C, with 6~8h of 130r/min stirring enzymolysis;It is described multiple
Synthase is trypsase and pepsin in mass ratio 1:1 mixes;
(2)After above-mentioned enzymolysis terminates, the enzymolysis mixture in enzymatic vessel is put into centrifuge, with 1200r/min centrifugations point
From 10~15min, supernatant is collected, counted by weight, it is 15% chlorine to take 60~65 portions of supernatants, 40~45 parts of mass fractions
Change calcium solution, 12~16 parts of glycine and 4~7 parts of ethylenediamines, be put into reactor, 80~85 DEG C of design temperature, with 150r/
2~3h of min stirring reactions;
(3)After above-mentioned reaction terminates, room temperature is naturally cooled to, discharging is collected out material, filtered to going out material, collected
Filtrate, by the sodium alginate of filtered fluid and filtered fluid quality 30~35% be put into mixer with 200r/min stirrings 10~
15min, adds the hypericin of sodium alginate quality 10~15%, continues to stir 40~45min, collects stirring mixture,
Obtain spacetabs type seed soak base fluid;
(4)Count by weight, take 45~50 parts of above-mentioned spacetabs type seed soak base fluids, 8~11 parts of distilled water, 5~7 parts of phosphorus
Sour hydrogen dipotassium and 1~3 part of 6-benzyl aminopurine, are put into glass reaction kettle, are protected using oxygen, with 160r/min stirring 1~
2h, collects out material, you can must improve wheat frost resistance spacetabs type seed soak.
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN108925379A (en) * | 2018-07-30 | 2018-12-04 | 成都市农林科学院 | A kind of overwintering grafting seedlings-growing method of wax gourd |
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CN108925379A (en) * | 2018-07-30 | 2018-12-04 | 成都市农林科学院 | A kind of overwintering grafting seedlings-growing method of wax gourd |
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Application publication date: 20170613 |