CN105145603A - Composite wheat seed soaking agent as well as preparation method, usage method and application thereof - Google Patents
Composite wheat seed soaking agent as well as preparation method, usage method and application thereof Download PDFInfo
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- CN105145603A CN105145603A CN201510500471.3A CN201510500471A CN105145603A CN 105145603 A CN105145603 A CN 105145603A CN 201510500471 A CN201510500471 A CN 201510500471A CN 105145603 A CN105145603 A CN 105145603A
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Abstract
The invention discloses a composite wheat seed soaking agent as well as a preparation method, a usage method and an application thereof and belongs to the field of wheat seed soaking agents. The composite wheat seed soaking agent mainly comprises uniconazole, bachmedesh and diphenylurea. The invention further discloses the preparation method, the usage method and the application of the composite wheat seed soaking agent. The uniconazole, the bachmedesh and the diphenylurea are subjected to ternary compounding, then a compound is diluted with a corn pollen liquid and used for processing wheat seeds, so that the negative effects caused by usage of single component can be eliminated more effectively, the obvious synergistic interaction function is realized through compatibility of four components, germinated seedlings are short and strong, root systems are developed, the lodging resistance is enhanced, and stress resistance such as insect resistance, drought resistance, salt tolerance and the like is remarkably improved. With the adoption of the composite wheat seed soaking agent for processing the wheat seeds, the wheat planting range can be broadened, and effects of production increase, yield stabilization and the like are realized.
Description
Technical field
The present invention relates to seed soaking agent, particularly relate to being active ingredient with various plants growth regulator and zasiokaurin liquid, urging strong at short root and improve the compound wheat seed soaking agent having remarkable synergy effect in lodging resistance, resistance etc. of a kind of applicable wheat, belong to wheat seed soaking agent field.
Background technology
Wheat is one of main cereal crops of China.Wheat is subject to the infringement of the adverse circumstance such as arid, salt damage in kind of a seedling stage, and lodging problem easily appears in Later growth.At present, educate strong sprout and resistant to lodging be the important means expanding wheat planting scope, volume increase, stable yields.
For solving the problem, except field management measure, common method is seed pelleting, seed dressing and seed soaking.Adopt seed soaking agent process seed, production technology is simple, easy to use, treating capacity large, and seed can not only be allowed fully to absorb various active ingredient, also has the effect that vernalization is early sent out.
CN104286025A discloses a kind of composite efficient wheat seed soaking agent and using method thereof, involved plant growth regulator is uniconazole P and indolebutyric acid, there is certain effect in wheat growth-promoting, do not improve effect of the resistance such as wheat resistance to insects.CN104012534A discloses the cold-resistant conditioning agent of a grow wheat, and involved plant growth regulator is salicylic acid and brassin lactones, and this conditioning agent is urged in lodging resistance of strengthening, increasing wheat etc. barely satisfactory at short root, have much room for improvement.CN104186065A discloses grow wheat strong sprout degeneration-resistant seed-soaking method, and involved plant growth regulator is chlormequat, and it does not substantially have effect in promotion growth of wheat roots.
In order to overcome the many defects existing for existing wheat seed soaking agent, promote growth of wheat roots, improve the lodging resistance of Wheat Species seedling stage stress resistance and Later growth, urgently need a kind of root of can effectively urging of development to urge to strengthen, increase the lodging resistance of wheat and the compound wheat seed soaking agent of resistance.
Summary of the invention
Technical problem to be solved by this invention overcomes the deficiencies in the prior art, provides a kind of root of can effectively urging to urge to strengthen, increase lodging resistance and improve the compound wheat seed soaking agent of the anti-adversities such as resistance to insects, drought resistance and salt resistance.
Technical problem to be solved by this invention is achieved through the following technical solutions:
A kind of compound wheat seed soaking agent, is grouped into by following one-tenth: uniconazole P, Bachmedesh, diphenylurea and acetic acid.
Preferably, by mass percentage, the consumption of each composition is: uniconazole P 0.4%-0.5%, Bachmedesh 1.2%-1.5%, diphenylurea 0.5%-0.6%, surplus are acetic acid;
Preferred, the consumption of each composition is: uniconazole P 0.5%, Bachmedesh 1.5%, diphenylurea 0.5%.
Uniconazole P is a kind of plant growth regulator with the short strong effect of dwarfing, effectively can reduce plant height thus improve lodging resistance, but is used alone the counter productive that uniconazole P has speed of germination reduction, root length shortens.In order to eliminate the counter productive using single plant growth regulator, it is compatible that uniconazole P and DCPTA, diethyl aminoethyl hexanoate, 6-BA, Bachmedesh, diphenylurea etc. are had the composition that hestening rooting germinates by the present invention, expect that screening obtains one and effectively can not only reduce plant height, effectively can also urge the compound wheat seed soaking agent that root urgees to strengthen, increase lodging resistance.The present invention is found by screening experiment, uniconazole P and Bachmedesh, diphenylurea being carried out the composite compound wheat seed soaking agent obtained arranges in pairs or groups after zasiokaurin liquid uses, and is reducing plant height, hestening rooting and in resistance to insects, drought resistance, salt resistance etc., is all significantly being better than other compounded combination.Uniconazole P and Bachmedesh, diphenylurea are carried out composite, effectively can eliminate the counter productive using single medicament, uniconazole P did not lose efficacy to the suppression of plant height and overcame the defect that Bachmedesh and diphenylurea make plant height excessively rapid growth, uniconazole P to the suppression of root growth effectively by Bachmedesh and diphenylurea clear up, three's compatibility significantly enhances again the resistance of wheat together, has extremely significant Synergistic effect.
Impact between plant growth regulator is very complicated, may there is the multiple impact such as synergy, antagonism, so that likely there is the situation that binary built is better than ternary built.In addition, from the viewpoint of cost and preparation processing, reduce medicament kind and also there is obvious advantage; Given this, the present invention designs binary built test further and does further inspection to the effect of ternary built.Result of the test is visible, and the Be very effective that uniconazole P, Bachmedesh and diphenylurea carry out ternary built is better than other binary built scheme (difference reaches pole significance level); Composite score is visible, and the composite effect of uniconazole P, Bachmedesh and diphenylurea all obviously will be better than the compound scheme than other, confirms and uniconazole P and Bachmedesh and diphenylurea is carried out compositely have significant Synergistic effect.
To be made up of uniconazole P, Bachmedesh and diphenylurea and to have on the basis of Synergistic determining compound wheat seed soaking agent, the present invention finds further, the different amounts proportioning of uniconazole P, Bachmedesh and diphenylurea, for the impact also highly significant of effect of soaking seed.The present invention is found by a large amount of screening experiments, and three kinds of compositions carry out compatibility according to following amount ranges and have good synergy:
By mass percentage, uniconazole P 0.4%-0.5%, Bachmedesh 1.2%-1.5%, diphenylurea 0.5%-0.6%, surplus are acetic acid;
Wherein, three kinds of compositions carry out compatibility according to following consumption and have best synergy:
Uniconazole P 0.5%, Bachmedesh 1.5%, diphenylurea 0.5%, surplus are acetic acid.
Invention further provides a kind of method preparing described compound wheat seed soaking agent, the method comprises: join in acetic acid by uniconazole P, Bachmedesh and diphenylurea, stir, to obtain final product.
In order to reach better technique effect, described acetic acid is preferably the acetic acid of content >=75%.
The present invention still further provides described compound wheat seed soaking agent and urgees to strengthen, increase lodging resistance and improve the application in resistance at reduction Plant Height in Wheat, short root, comprise: by described compound wheat seed soaking agent dilute with water, obtain the compound wheat seed soaking agent after diluting; Compound wheat seed soaking agent after being diluted by wheat seed is soaked seed;
Wherein, by described compound wheat seed soaking agent dilute with water 5000-15000 doubly, be preferably 8000-12000 doubly, be more preferably 10000 times;
According to mass ratio meter, the ratio of wheat seed and the compound wheat seed soaking agent liquid after diluting is preferably 1:3; Described immersion is preferably soaked seed 24 hours at 20 DEG C of-30 DEG C of temperature.
The present invention finds very unexpectedly, after uniconazole P, Bachmedesh, diphenylurea and zasiokaurin liquid phase compatibility, can the raising seed soaking agent of the highly significant effect urging to strengthen at short root, increase lodging resistance and improve in the resistance such as resistance to insects, drought resistance and salt resistance.
Zasiokaurin contains protein 30.43%, fat 2.5%, starch 22.4%, reducing sugar 6.88%, non reducing sugar 7.31%, nucleic acid 1.63%.Containing 10 multivitamin such as Va, Vb, Vc, Ve in zasiokaurin, more than the 20 kind of mineral elements such as calcium, iron, potassium, sodium, 18 material such as seed amino acid and flavonoids such as proline, asparatate, glutamic acid, lysine.In North China of China, Wheat Maize Rotation is the common tillage method in wheat planting district.Zasiokaurin is rich in multiple beneficial material, makes pollen liquid after being collected by zasiokaurin, and for the treatment of the wheat seed connecing stubble sowing, arrangement of time is reasonable, can also turn waste into wealth.Use zasiokaurin liquid to carry out to wheat seed the growth rate that seed soaking can improve wheat, but be used alone zasiokaurin liquid and make resistance to insects and the lodging resistance in later stage (plant height increases too fast) that seed soaking can reduce wheat.
In order to screen the optimum amount proportioning of uniconazole P, Bachmedesh, diphenylurea and zasiokaurin liquid, the consumption proportion of the present invention to four kinds of constituents screens, and more different consumptions is for the impact that wheat reduces plant height, short root urgees to strengthen, increase the aspect such as lodging resistance and resistance, result of the test shows, the good compound scheme of effect is uniconazole P 0.4%-0.5%+ Bachmedesh 1.2%-1.5%+ diphenylurea 0.5%-0.6%, with zasiokaurin liquid (zasiokaurin stoste dilutes 5 times) dilution 10000 times, this consumption proportion is adopted to promote will significantly be better than in the effect such as root growth and raising resistance other consumption proportion (difference reaches significance level), wherein, uniconazole P 0.5%+ Bachmedesh 1.5%+ diphenylurea 0.5%, the effect of diluting 10000 times with zasiokaurin liquid (zasiokaurin stoste dilutes 5 times) is that in all compound schemes, effect is best, significantly be better than other consumption proportion (difference reaches pole significance level) in this compound scheme.
Therefore, present invention also offers another compound wheat seed soaking agent, be made up of composition I and composition II; According to mass ratio meter, composition I: composition II=1:5000-15000; Preferably, composition I: composition II=1:7500-12500; Most preferred, composition I: composition II=1:10000.
Described composition I is made up of uniconazole P, Bachmedesh, diphenylurea and acetic acid; By mass percentage, in composition I, the consumption of each composition is: uniconazole P 0.4%-0.5%, Bachmedesh 1.2%-1.5%, diphenylurea 0.5%-0.6%, surplus are acetic acid; Preferably, the consumption of each composition is: uniconazole P 0.5%, Bachmedesh 1.5%, diphenylurea 0.5%;
Described composition II is zasiokaurin liquid.
Described zasiokaurin liquid obtains after zasiokaurin stoste dilute with water, and preferably, described zasiokaurin liquid obtains after zasiokaurin stoste dilute with water 2-20 times; Preferred, described zasiokaurin liquid obtains after zasiokaurin stoste dilute with water 2-8 times; Most preferred, described zasiokaurin liquid obtains after zasiokaurin stoste dilute with water 5 times.
Described zasiokaurin stoste preferably prepares in accordance with the following methods:
(1) zasiokaurin is gathered;
(2) deposit freezing at the temperature of-10 DEG C ~-20 DEG C for zasiokaurin;
(3) zasiokaurin after freezing depositing is joined in hot water stir, after cooling and get final product.
Preferably, gather zasiokaurin in step (1) when corn full-bloom stage, be exposed to the sun the zasiokaurin that the same day gathers 1-2 hour in the sun;
Preferably, deposit freezing at the temperature of-20 DEG C for zasiokaurin in step (2);
Preferably, in step (3), the freezing zasiokaurin deposited at least 30 days is joined in 80-100 DEG C of hot water of 3-20 times of quality and stir, after cooling and get final product; Preferred further, the freezing zasiokaurin deposited at least 30 days is joined in 100 DEG C of hot water of 10 times of quality and stir.
The invention provides a kind of method preparing this compound wheat seed soaking agent, comprising: composition I and composition II are mixed, to obtain final product.
Accordingly, the invention provides this compound wheat seed soaking agent to urge to strengthen, increase lodging resistance or improve the application in resistance at reduction Plant Height in Wheat, short root; Described application comprises: soaked seed by the described compound wheat seed soaking agent of wheat seed; Wherein, according to mass ratio meter, the ratio of wheat seed and compound wheat seed soaking agent liquid is preferably 1:3; Described immersion is preferably soaked seed 24 hours at 20 DEG C of-30 DEG C of temperature.
Uniconazole P and Bachmedesh, diphenylurea carry out composite by compound wheat seed soaking agent of the present invention, zasiokaurin liquid is coordinated to use again, effectively can eliminate the counter productive using single medicament, four compatibilities have extremely significant Synergistic effect together, after its process wheat seed, seedling after sprouting short strong (plant height reduces 40%-60%, stem foot wide increase 100%-200%), well developed root system (the long 5%-15% of increasing of root, root number increase 10%-20%); Effectively urge root to urge to strengthen, increase lodging resistance, improve resistance (rate of damage reduces 75%-95%, drought resistance enhancing 1-2 times, salt resistance enhancing 1-2 doubly) simultaneously, finally reach the object expanding planting range, volume increase, stable yields.
Embodiment
Further describe the present invention below in conjunction with specific embodiment, advantage and disadvantage of the present invention will be more clear along with description.It should be understood that described embodiment is only exemplary, any restriction is not formed to scope of the present invention.It will be understood by those skilled in the art that and can modify to the details of technical solution of the present invention and form or replace down without departing from the spirit and scope of the present invention, but these amendments or replacement all fall into protection scope of the present invention.
The preparation of Preparative Example 1 zasiokaurin stoste
(1) in the sunny morning (9 .-11 point) of corn full-bloom stage, the maize male ears of interval clip (interlacing or every strain) loose powder about 50%, shakeout on clean Polypropylence Sheet or paper noon on the same day (11 .-14 point), be exposed to the sun 1-2 hour in the sun;
(2) pollen is shaken to cause drop concentrated on clean Polypropylence Sheet or paper gathering, sieve the impurity such as medicine of deflorating, load totally airtight vessel, deposit in freezer compartment of refrigerator (temperature is adjusted to-20 DEG C);
(3) the freezing zasiokaurin deposited 30 days is taken out, add 100 DEG C of hot water of 10 times of quality fast and stir, naturally cooling to room temperature, obtain zasiokaurin stoste.
The preparation of Preparative Example 2 zasiokaurin stoste
(1) in the sunny morning (9 .-11 point) of corn full-bloom stage, the maize male ears of interval clip (interlacing or every strain) loose powder about 50%, shakeout on clean Polypropylence Sheet or paper noon on the same day (11 .-14 point), be exposed to the sun 2-3 hour in the sun;
(2) pollen is shaken to cause drop concentrated on clean Polypropylence Sheet or paper gathering, sieve the impurity such as medicine of deflorating, load totally airtight vessel, deposit in freezer compartment of refrigerator (temperature is adjusted to-15 DEG C);
(3) the freezing zasiokaurin deposited 40 days is taken out, add 90 DEG C of hot water of 6 times of quality fast and stir, naturally cooling to room temperature, obtain zasiokaurin stoste.
The preparation of Preparative Example 3 zasiokaurin stoste
(1) in the sunny morning (9 .-11 point) of corn full-bloom stage, the maize male ears of interval clip (interlacing or every strain) loose powder about 50%, shakeout on clean Polypropylence Sheet or paper noon on the same day (11 .-14 point), be exposed to the sun 2-3 hour in the sun;
(2) pollen is shaken to cause drop concentrated on clean Polypropylence Sheet or paper gathering, sieve the impurity such as medicine of deflorating, load totally airtight vessel, deposit in freezer compartment of refrigerator (temperature is adjusted to-10 DEG C);
(3) the freezing zasiokaurin deposited 38 days is taken out, add 80 DEG C of hot water of 12 times of quality fast and stir, naturally cooling to room temperature, obtain zasiokaurin stoste.
The preparation of embodiment 1 compound wheat seed soaking agent
Each composition (unit: g): uniconazole P 0.5, Bachmedesh 1.5, diphenylurea 0.5 is taken by following quality; Above-mentioned each composition is joined in acetic acid (content >=75%) and supplies acetic acid to 100g, stir, to obtain final product.
The preparation of embodiment 2 compound wheat seed soaking agent
Each composition (unit: g): uniconazole P 0.4, Bachmedesh 1.2, diphenylurea 0.5 is taken by following quality; Above-mentioned each composition is joined in acetic acid and supplies acetic acid (content >=75%) to 100g, stir, to obtain final product.
The preparation of embodiment 3 compound wheat seed soaking agent
Each composition (unit: g): uniconazole P 0.5, Bachmedesh 1.5, diphenylurea 0.6 is taken by following quality; Above-mentioned each composition is joined in acetic acid and supplies acetic acid (content >=75%) to 100g, stir, to obtain final product.
The preparation of embodiment 4 compound wheat seed soaking agent
Each composition (unit: g): uniconazole P 0.4, Bachmedesh 1.5, diphenylurea 0.6 is taken by following quality; Above-mentioned each composition is joined in acetic acid and supplies acetic acid (content >=75%) to 100g, stir, to obtain final product.
The preparation of embodiment 5 compound wheat seed soaking agent
Each composition (unit: g): uniconazole P 0.5, Bachmedesh 1.2, diphenylurea 0.5 is taken by following quality; Above-mentioned each composition is joined in acetic acid and supplies acetic acid (content >=75%) to 100g, stir, to obtain final product.
The preparation of embodiment 6 compound wheat seed soaking agent
The preparation of composition I: take each composition (unit: g): uniconazole P 0.5, Bachmedesh 1.5, diphenylurea 0.5 by following quality; Above-mentioned each composition is joined in acetic acid (content >=75%) and supplies acetic acid to 100g, stir, to obtain final product.
The preparation of composition II: the zasiokaurin stoste dilute with water 5 times prepared by Preparative Example 1, obtains zasiokaurin liquid.
Composition I and composition II are mixed according to the mass ratio of 1:10000, to obtain final product.
The preparation of embodiment 7 compound wheat seed soaking agent
The preparation of composition I: take each composition (unit: g): uniconazole P 0.4, Bachmedesh 1.2, diphenylurea 0.5 by following quality; Above-mentioned each composition is joined in acetic acid and supplies acetic acid (content >=75%) to 100g, stir, to obtain final product.
The preparation of composition II: the zasiokaurin stoste dilute with water 8 times prepared by Preparative Example 2, obtains zasiokaurin liquid.
Composition I and composition II are mixed according to the mass ratio of 1:7500, to obtain final product.
The preparation of embodiment 8 compound wheat seed soaking agent
The preparation of composition I: take each composition (unit: g): uniconazole P 0.5, Bachmedesh 1.5, diphenylurea 0.6 by following quality; Above-mentioned each composition is joined in acetic acid and supplies acetic acid (content >=75%) to 100g, stir, to obtain final product.
The preparation of composition II: the zasiokaurin stoste dilute with water 8 times prepared by Preparative Example 3, obtains zasiokaurin liquid.
Composition I and composition II are mixed according to the mass ratio of 1:12500, to obtain final product.
The screening test of the constituent of test example 1 compound wheat seed soaking agent
One, test method
(1) the formula composition of each process compound seed soaking agent
Find that according to primary screening test the plant growth regulator such as DCPTA, diethyl aminoethyl hexanoate, 6-BA, Bachmedesh, diphenylurea and zasiokaurin liquid have effect of hestening rooting germination; The compositions such as uniconazole P and DCPTA, diethyl aminoethyl hexanoate, 6-BA, Bachmedesh, diphenylurea, zasiokaurin liquid are carried out according to the mode of table 1 the compound wheat seed soaking agent that compatibility obtains different disposal by this experiment.
Table 1 respectively processes the formula composition of compound seed soaking agent and prepares and extension rate
| Process | The compound method of seed soaking agent | Extension rate | Dilution |
| 1 | Uniconazole P 0.5g, Bachmedesh 1.5g, diphenylurea 0.5g, 100g supplied by acetic acid | 10000 | Zasiokaurin stoste dilutes 5 times |
| 2 | Uniconazole P 0.5g, DCPTA 1.5g, diphenylurea 0.5g, 100g supplied by acetic acid | 10000 | Zasiokaurin stoste dilutes 5 times |
| 3 | Uniconazole P 0.5g, diethyl aminoethyl hexanoate 12g, diphenylurea 0.5g, 100g supplied by acetic acid | 10000 | Zasiokaurin stoste dilutes 5 times |
| 4 | Uniconazole P 0.5g, Bachmedesh 1.5g, 6-BA0.5g, 100g supplied by acetic acid | 10000 | Zasiokaurin stoste dilutes 5 times |
| 5 | Uniconazole P 0.5g, DCPTA 1.5g, 6-BA0.5g, 100g supplied by acetic acid | 10000 | Zasiokaurin stoste dilutes 5 times |
| 6 | Uniconazole P 0.5g, diethyl aminoethyl hexanoate 12g, 6-BA0.5g, 100g supplied by acetic acid | 10000 | Zasiokaurin stoste dilutes 5 times |
| 7 | Blank (acetic acid) | 10000 | Zasiokaurin stoste dilutes 5 times |
| 8 | Uniconazole P 0.5g, Bachmedesh 1.5g, diphenylurea 0.5g, 100g supplied by acetic acid | 10000 | Running water |
| 9 | Uniconazole P 0.5g, DCPTA 1.5g, diphenylurea 0.5g, 100g supplied by acetic acid | 10000 | Running water |
| 10 | Uniconazole P 0.5g, diethyl aminoethyl hexanoate 12g, diphenylurea 0.5g, 100g supplied by acetic acid | 10000 | Running water |
| 11 | Uniconazole P 0.5g, Bachmedesh 1.5g, 6-BA0.5g, 100g supplied by acetic acid | 10000 | Running water |
| 12 | Uniconazole P 0.5g, DCPTA 1.5g, 6-BA0.5g, 100g supplied by acetic acid | 10000 | Running water |
| 13 | Uniconazole P 0.5g, diethyl aminoethyl hexanoate 12g, 6-BA0.5g, 100g supplied by acetic acid | 10000 | Running water |
| 14 | Blank (acetic acid) | 10000 | Running water |
| 15 | Uniconazole P 3g, indolebutyric acid 30g, white vitriol 534g | 5000 | Running water |
(2) sand culture test
The wheat seed of capital winter 17 kind is selected to carry out sand culture test.Select the quartz sand that particle diameter is 0.1mm-0.5mm, water content is 60%-80% to be matrix, length, width and height are the transparent plastic germination box with cover of 12cm × 12cm × 6cm is that culture vessel carries out germination test.Test totally 15 process (see table 1), acetic acid is blank, often kind of seed soaking agent all carry out unified seed soaking (seed disinfection, clean after, after the seed soaking agent of each process converts dilution by extension rate, every 3kg liquid leaching 1kg seed, soaks seed 24 hours for 20 DEG C-30 DEG C, the clean liquid sowing of filter after seed soaking), repeat for 4 times, random district group arranges.Every box sows 50, temperature 25 DEG C-30 DEG C, and humidity 40%-60%, light: cultivate under the condition of dark=7:5, from the 2d that sprouts, water running water 10ml/ box every day.After planting 7d dichotomy gets 25 strain seedling, measures plant height, stem foot is wide, root is long, root number.
(3) Drought resistant experiment
The wheat seed of capital winter 17 kind is selected to carry out Drought resistant experiment.Culture vessel is the culture dish of diameter 10cm, and the water content of matrix soil is 15% of saturation moisture content, and 50 seeds broadcast by each culture dish.Test totally 15 process (see table 1), acetic acid is blank, and often kind of seed soaking agent all carries out unified seed soaking (same to sand culture test), and repeat for 4 times, random district group arranges.Temperature 25 DEG C-30 DEG C, humidity 20%-40%, light: cultivate under the condition of dark=7:5, after planting 10d dichotomy gets 25 strain seedling, measures root long.
(4) Salt tolerance
The wheat seed of capital winter 17 kind is selected to carry out Salt tolerance.Test totally 15 process (see table 1), acetic acid is blank, and often kind of seed soaking agent all carries out unified seed soaking (same to sand culture test).Each culture dish (diameter 10cm) spreads 3 layers of blotting paper, pours 1mol/LNaCl aqueous solution 10ml into, puts into 50 selected, the seed of sprouting consistent, and repeat for 4 times, random district group arranges.Temperature 25 DEG C-30 DEG C, humidity 40%-60%, light: cultivate under the condition of dark=7:5, after planting 7d dichotomy gets 25 strain seedling, measures root long.
(5) Insect resistance assay
Select the wheat seed of capital winter 17 kind to carry out Insect resistance assay, carried out in 2013 in Huang Changpu village, Tongzhou District, Beijing City, preceding crop is corn, and test totally 15 process (see table 1), acetic acid is blank, and seeding quantity is 12.5kg/ mu.The group arrangement of random district, repeats for 4 times, every plot area 30m
2, between Qu Yuqu, establish protection row 1m.Sowing 45 Tian Houmei communities takes diagonal to fix a point, 3 samplings, look into the capable length of 1m at often, investigation basic seedling amount and killed seedling number, the seedling bitten by soil insect (as cutworm, grub, wireworm, mole cricket etc.) or kill is killed seedling, calculates rate of damage (rate of damage=killed seedling number/basic seedling amount × 100%).
Two, result of the test
Sand culture test the results are shown in Table 2-table 6, and resistance test (drought resisting, salt tolerant, pest-resistant) the results are shown in Table 7-table 10.
Result of the test shows, by the seed soaking agent (processing 1) after uniconazole P and Bachmedesh, diphenylurea and zasiokaurin liquid compatibility, compared to contrast (process 14), the plant height reduction by 44.5% of wheat seedling, the wide increase of stem foot 167.9%, the long increase by 7.4% of root, root number increase by 10.8%, and resistance to insects increases (rate of damage reduces by 93.3%), drought resistance strengthens (the long increase of root 148.3%), salt resistance enhancing (the long increase by 157.7% of root).Uniconazole P can be alleviated by Bachmedesh, diphenylurea and zasiokaurin liquid the suppression of root, does not lose efficacy to the suppression of plant height; Visible according to result of the test, zasiokaurin liquid of being arranged in pairs or groups by compound seed soaking agent after uniconazole P and Bachmedesh, diphenylurea compatibility uses, at reduction plant height, short root is short strong, increase the composite composition that the Be very effective of the aspect such as lodging resistance and resistance is better than other and (namely process 2-15, difference reaches pole significance level), this illustrates, uniconazole P and Bachmedesh, diphenylurea and zasiokaurin liquid phase compatibility is had extremely significant Synergistic effect.
Table 2 sand culture test result
| Process | Plant height (cm) | Stem foot wide (mm) | Root long (cm) | Root number |
| 1 | 5.21±0.1Ff | 3.49±0.13Aa | 11.4±0.36Aa | 5.02±0.18Aa |
| 2 | 6.38±0.11Ee | 2.33±0.07BCb | 10.43±0.41BCbcd | 4.29±0.09CDcd |
| 3 | 6.45±0.14Ee | 2.36±0.07Bb | 10.12±0.35Cd | 4.16±0.14DEd |
| 4 | 6.46±0.16Ee | 2.31±0.06BCbc | 10.15±0.28Cd | 4.23±0.13Dd |
| 5 | 8.74±0.31Cc | 1.77±0.07Gf | 7.83±0.23Ef | 3.63±0.14Gf |
| 6 | 8.68±0.25Cc | 1.74±0.07Gf | 8.16±0.28Ef | 3.65±0.05Gf |
| 7 | 10.89±0.27Aa | 2.32±0.06BCb | 10.73±0.35Bb | 4.69±0.08Bb |
| 8 | 6.32±0.17Ee | 2±0.03EFe | 10.24±0.26BCcd | 4.67±0.13CDd |
| 9 | 7.44±0.19Dd | 2.14±0.08Dd | 9.19±0.33De | 3.87±0.1Fe |
| 10 | 7.66±0.27Dd | 2.21±0.05CDcd | 9.2±0.24De | 3.95±0.06EFe |
| 11 | 7.69±0.31Dd | 2.14±0.07DEd | 9.04±0.2De | 3.88±0.11Fe |
| 12 | 8.77±0.28Cc | 1.78±0.04Gf | 8±0.17Ef | 3.59±0.12Gf |
| 13 | 8.7±0.09Cc | 1.75±0.07Gf | 8.09±0.23Ef | 3.59±0.12Gf |
| 14 | 9.75±0.19Bb | 1.97±0.06Fe | 10.61±0.42BCbc | 4.44±0.1Cc |
| 15 | 8.75±0.29Cc | 1.77±0.07Gf | 8.08±0.26Ef | 3.52±0.06Gf |
Table 3 sand culture test plant height variance analysis table
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| Between district's group | 0.0943 | 3 | 0.0314 | 0.618 | 0.607 |
| Between process | 129.3859 | 14 | 9.2418 | 181.756 | 0 |
| Error | 2.1356 | 42 | 0.0508 | ||
| Total variation | 131.6158 | 59 |
The wide variance analysis table of table 4 sand culture test stem foot
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| Between district's group | 0.001 | 3 | 0.0003 | 0.066 | 0.9778 |
| Between process | 10.9197 | 14 | 0.78 | 149.774 | 0 |
| Error | 0.2187 | 42 | 0.0052 | ||
| Total variation | 11.1394 | 59 |
The long variance analysis table of table 5 sand culture test root
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| Between district's group | 0.7875 | 3 | 0.2625 | 3.373 | 0.0271 |
| Between process | 78.8251 | 14 | 5.6304 | 72.347 | 0 |
| Error | 3.2686 | 42 | 0.0778 | ||
| Total variation | 82.8812 | 59 |
Table 6 sand culture test root number variance analysis table
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| Between district's group | 0.042 | 3 | 0.014 | 1.122 | 0.351 |
| Between process | 12.3475 | 14 | 0.882 | 70.672 | 0 |
| Error | 0.5241 | 42 | 0.0125 | ||
| Total variation | 12.9136 | 59 |
Table 7 drought resisting, salt tolerant, Insect resistance assay result
| Process | Drought resistant experiment root long (cm) | Salt tolerance root long (cm) | Rate of damage (%) |
| 1 | 6.49±0.19Aa | 7.9±0.18Aa | 0.5±0.01Hh |
| 2 | 5.52±0.15Dcd | 7.04±0.31Bb | 2.84±0.1Cc |
| 3 | 5.59±0.12CDc | 7±0.25Bb | 2.84±0.04Cc |
| 4 | 5.81±0.04BCb | 6.99±0.14Bb | 2.74±0.1Cc |
| 5 | 5.2±0.15Ee | 6.3±0.1Cc | 1.99±0.06Dd |
| 6 | 5.35±0.12DEde | 6.35±0.09Cc | 2±0.05Dd |
| 7 | 4.5±0.06Hh | 4.84±0.09Ff | 11.71±0.21Aa |
| 8 | 5.95±0.09Bb | 7.47±0.29Bb | 0.88±0.02Gg |
| 9 | 4.92±0.15Ff | 5.91±0.06Dd | 1.13±0.02Ff |
| 10 | 4.92±0.08Ff | 5.84±0.16Dd | 1.11±0.02Ff |
| 11 | 4.88±0.08FGf | 5.92±0.12Dd | 1.12±0.02Ff |
| 12 | 4.68±0.18GHg | 5.42±0.03Ee | 1.66±0.02Ee |
| 13 | 4.68±0.13GHgh | 5.41±0.16Ee | 1.6±0.05Ee |
| 14 | 4.24±0.1Ii | 4.83±0.09Ff | 8.97±0.36Bb |
| 15 | 4.68±0.17FGHg | 5.4±0.21Ee | 1.6±0.05Ee |
The long variance analysis table of table 8 Drought resistant experiment root
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| Between district's group | 0.0635 | 3 | 0.0212 | 1.328 | 0.278 |
| Between process | 21.3899 | 14 | 1.5278 | 95.815 | 0 |
| Error | 0.6697 | 42 | 0.0159 | ||
| Total variation | 22.1231 | 59 |
The long variance analysis table of table 9 Salt tolerance root
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| Between district's group | 0.2612 | 3 | 0.0871 | 3.494 | 0.0237 |
| Between process | 49.5783 | 14 | 3.5413 | 142.123 | 0 |
| Error | 1.0465 | 42 | 0.0249 | ||
| Total variation | 50.886 | 59 |
Table 10 Insect resistance assay rate of damage variance analysis table
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| Between district's group | 0.0551 | 3 | 0.0184 | 1.382 | 0.2615 |
| Between process | 561.4419 | 14 | 40.103 | 3018.9 | 0 |
| Error | 0.5579 | 42 | 0.0133 | ||
| Total variation | 562.0549 | 59 |
The inspection test of the constituent of test example 2 compound wheat seed soaking agent
One, test method
(1) the formula composition of each process compound seed soaking agent
The result of the constituent screening test of test example 1 shows, can eliminate the inhibitory action of uniconazole P to root system after Bachmedesh, diphenylurea and zasiokaurin liquid are composite, and other compound scheme does not all have similar effect.In view of the impact between plant growth regulator is very complicated, the multiple impact such as synergy, antagonism may be there is, the situation that binary built is better than ternary built may be there is.In addition, from the viewpoint of cost and preparation processing, reduce medicament kind and also have obvious advantage, so design binary built and ternary built test according to the situation of test example 1, the consumption with reference to table 11 designs.
Table 11 respectively processes the formula composition of compound seed soaking agent and prepares and extension rate
| Process | The compound method of seed soaking agent | Extension rate | Dilution |
| 1 | Uniconazole P 0.5g, Bachmedesh 1.5g, diphenylurea 0.5g, 100g supplied by acetic acid | 10000 | Zasiokaurin stoste dilutes 5 times |
| 2 | Uniconazole P 0.5g, Bachmedesh 1.5g, 100g supplied by acetic acid | 10000 | Zasiokaurin stoste dilutes 5 times |
| 3 | Uniconazole P 0.5g, DCPTA 1.5g, 100g supplied by acetic acid | 10000 | Zasiokaurin stoste dilutes 5 times |
| 4 | Uniconazole P 0.5g, diethyl aminoethyl hexanoate 12g, 100g supplied by acetic acid | 10000 | Zasiokaurin stoste dilutes 5 times |
| 5 | Uniconazole P 0.5g, diphenylurea 0.5g, 100g supplied by acetic acid | 10000 | Zasiokaurin stoste dilutes 5 times |
| 6 | Uniconazole P 0.5g, 6-BA0.5g, 100g supplied by acetic acid | 10000 | Zasiokaurin stoste dilutes 5 times |
| 7 | Uniconazole P 0.5g, 100g supplied by acetic acid | 10000 | Zasiokaurin stoste dilutes 5 times |
| 8 | Blank (acetic acid) | 10000 | Zasiokaurin stoste dilutes 5 times |
(2) sand culture test
Concrete grammar is with test example 1, and measurement index is that root is long.
(3) Drought resistant experiment
Concrete grammar is with test example 1.
(4) Salt tolerance
Concrete grammar is with test example 1.
(5) Insect resistance assay
Concrete grammar is with test example 1.
Two, result of the test
Result of the test is in Table 12-table 16.
Visible according to result of the test, the compound scheme that the composite Be very effective of uniconazole P, Bachmedesh, diphenylurea and pollen liquid is better than other (namely processes 2-8, difference reaches pole significance level), binding tests example 1 considers, the composite effect of uniconazole P, Bachmedesh and diphenylurea obviously will be better than other compound scheme, and this shows there is obvious Synergistic effect between three plant growth regulators in this compound scheme and zasiokaurin liquid.
Table 12 constituent inspection test result
| Process | Sand culture test root long (cm) | Drought resistant experiment root long (cm) | Salt tolerance root long (cm) | Rate of damage (%) |
| 1 | 12.2±0.28Aa | 6.76±0.24Aa | 7.35±0.1Aa | 0.69±0.02Ee |
| 2 | 8.81±0.28CDc | 5.84±0.2BCbc | 6.15±0.13BCbc | 1.74±0.04Dd |
| 3 | 8.74±0.05CDc | 5.63±0.17Ccd | 6.01±0.15BCcd | 2.22±0.07Cc |
| 4 | 8.77±0.26CDc | 5.98±0.24Bb | 6.17±0.27BCbc | 1.73±0.07Dd |
| 5 | 8.92±0.37Cc | 5.8±0.1BCbc | 6.25±0.13Bb | 1.71±0.05Dd |
| 6 | 8.29±0.34Dd | 5.56±0.17Cd | 5.89±0.12Cd | 2.17±0.09Cc |
| 7 | 6.32±0.11Ee | 4.82±0.18De | 5.34±0.13De | 6.07±0.27Bb |
| 8 | 11±0.32Bb | 4.69±0.12De | 4.98±0.17Ef | 8.34±0.2Aa |
The long variance analysis table of table 13 sand culture test root
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| Between district's group | 0.0987 | 3 | 0.0329 | 0.414 | 0.7445 |
| Between process | 87.8425 | 7 | 12.5489 | 158.079 | 0 |
| Error | 1.6671 | 21 | 0.0794 | ||
| Total variation | 89.6083 | 31 |
The long variance analysis table of table 14 Drought resistant experiment root
The long variance analysis table of table 15 Salt tolerance root
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| Between district's group | 0.0366 | 3 | 0.0122 | 0.455 | 0.7165 |
| Between process | 13.7669 | 7 | 1.9667 | 73.434 | 0 |
| Error | 0.5624 | 21 | 0.0268 | ||
| Total variation | 14.3658 | 31 |
Table 16 Insect resistance assay rate of damage variance analysis table
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| Between district's group | 0.0221 | 3 | 0.0074 | 0.415 | 0.7438 |
| Between process | 197.6435 | 7 | 28.2348 | 1593.791 | 0 |
| Error | 0.372 | 21 | 0.0177 | ||
| Total variation | 198.0376 | 31 |
The consumption screening test of the constituent of test example 3 compound wheat seed soaking agent
One, test method
(1) the consumption design of the constituent of compound wheat seed soaking agent
According to consumption design 15 processed group of table 17.
Table 17 respectively processes the compound method of seed soaking agent
| Process | The compound method of seed soaking agent | Extension rate | Dilution |
| 1 | Uniconazole P 0.5g, Bachmedesh 1.5g, diphenylurea 0.5g, 100g supplied by acetic acid | 10000 | Zasiokaurin stoste dilutes 5 times |
| 2 | Uniconazole P 0.3g, Bachmedesh 1.5g, diphenylurea 0.5g, 100g supplied by acetic acid | 10000 | Zasiokaurin stoste dilutes 5 times |
| 3 | Uniconazole P 0.4g, Bachmedesh 1.5g, diphenylurea 0.5g, 100g supplied by acetic acid | 10000 | Zasiokaurin stoste dilutes 5 times |
| 4 | Uniconazole P 0.6g, Bachmedesh 1.5g, diphenylurea 0.5g, 100g supplied by acetic acid | 10000 | Zasiokaurin stoste dilutes 5 times |
| 5 | Uniconazole P 0.5g, Bachmedesh 0.9g, diphenylurea 0.5g, 100g supplied by acetic acid | 10000 | Zasiokaurin stoste dilutes 5 times |
| 6 | Uniconazole P 0.5g, Bachmedesh 1.2g, diphenylurea 0.5g, 100g supplied by acetic acid | 10000 | Zasiokaurin stoste dilutes 5 times |
| 7 | Uniconazole P 0.5g, Bachmedesh 1.8g, diphenylurea 0.5g, 100g supplied by acetic acid | 10000 | Zasiokaurin stoste dilutes 5 times |
| 8 | Uniconazole P 0.5g, Bachmedesh 1.5g, diphenylurea 0.4g, 100g supplied by acetic acid | 10000 | Zasiokaurin stoste dilutes 5 times |
| 9 | Uniconazole P 0.5g, Bachmedesh 1.5g, diphenylurea 0.6g, 100g supplied by acetic acid | 10000 | Zasiokaurin stoste dilutes 5 times |
| 10 | Uniconazole P 0.5g, Bachmedesh 1.5g, diphenylurea 0.7g, 100g supplied by acetic acid | 10000 | Zasiokaurin stoste dilutes 5 times |
| 11 | Uniconazole P 0.5g, Bachmedesh 1.5g, diphenylurea 0.5g, 100g supplied by acetic acid | 10000 | Zasiokaurin stoste 1 times dilution |
| 12 | Uniconazole P 0.5g, Bachmedesh 1.5g, diphenylurea 0.5g, 100g supplied by acetic acid | 10000 | Zasiokaurin stoste 2 times dilution |
| 13 | Uniconazole P 0.5g, Bachmedesh 1.5g, diphenylurea 0.5g, 100g supplied by acetic acid | 10000 | Zasiokaurin stoste 8 times dilution |
| 14 | Uniconazole P 0.5g, Bachmedesh 1.5g, diphenylurea 0.5g, 100g supplied by acetic acid | 10000 | Zasiokaurin stoste 9 times dilution |
| 15 | Uniconazole P 0.6g, Bachmedesh 0.9g, diphenylurea 0.7g, 100g supplied by acetic acid | 10000 | Zasiokaurin stoste dilutes 5 times |
(2) sand culture test
Concrete grammar is with test example 1, and measurement index is that root is long.
(3) Drought resistant experiment
Concrete grammar is with test example 1.
(4) Salt tolerance
Concrete grammar is with test example 1.
(5) Insect resistance assay
Concrete grammar is with test example 1.
Two, result of the test
Result of the test is in Table 18-table 22.
Visible according to result of the test, the good compound scheme of effect is: uniconazole P 0.4%-0.5%+ Bachmedesh 1.2%-1.5%+ diphenylurea 0.5%-0.6% collocation zasiokaurin liquid (zasiokaurin stoste dilutes 5 times), and this compound scheme is better than extraneous other scheme (difference reaches significance level) of this compound scheme at promotion root growth, resistance Be very effective; Wherein, the effect that uniconazole P 0.5%+ Bachmedesh 1.5%+ diphenylurea 0.5% arranges in pairs or groups zasiokaurin liquid (zasiokaurin stoste dilutes 5 times) is best in compound scheme, is significantly better than other formula (difference reaches pole significance level) in this compound scheme.
Table 18 constituent consumption result of the test
| Process | Sand culture test root long (cm) | Drought resistant experiment root long (cm) | Salt tolerance root long (cm) | Rate of damage (%) |
| 1 | 10.95±0.2Aa | 7.86±0.21Aa | 6.94±0.05Aa | 0.79±0.03Ee |
| 2 | 7.71±0.21Cc | 6.06±0.25DEFde | 5.75±0.18DEFGHfgh | 1.74±0.06Cc |
| 3 | 8.31±0.24Bb | 6.72±0.23BCb | 5.93±0.1BCDEFcdef | 1.4±0.05Dd |
| 4 | 6.78±0.21Dd | 6.11±0.18DEFde | 5.52±0.08GHij | 1.71±0.06Cc |
| 5 | 7.51±0.21Cc | 6.17±0.13DEFcd | 5.8±0.09CDEFGdefg | 1.71±0.04Cc |
| 6 | 8.57±0.19Bb | 6.82±0.22Bb | 6.04±0.16BCbc | 1.39±0.03Dd |
| 7 | 7.6±0.37Cc | 6.02±0.13EFde | 5.71±0.19EFGHghi | 1.7±0.04Cc |
| 8 | 7.41±0.15Cc | 6.4±0.05CDc | 5.79±0.18CDEFGefg | 1.72±0.03Cc |
| 9 | 8.38±0.29Bb | 6.82±0.12Bb | 5.96±0.06BCDEbcde | 1.38±0.03Dd |
| 10 | 7.55±0.19Cc | 6.11±0.24DEFde | 5.54±0.08GHhij | 1.72±0.04Cc |
| 11 | 7.54±0.23Cc | 6.13±0.27DEFcde | 5.67±0.24FGHghij | 1.69±0.04Cc |
| 12 | 8.38±0.24Bb | 6.83±0.29Bb | 6.01±0.09BCDbcd | 1.39±0.05Dd |
| 13 | 8.3±0.26Bb | 6.96±0.07Bb | 6.14±0.2Bb | 1.44±0.01Dd |
| 14 | 7.61±0.22Cc | 6.23±0.27DEcd | 5.7±0.03EFGHghij | 2±0.06Bb |
| 15 | 6.19±0.2Ee | 5.85±0.27Fe | 5.5±0.19Hj | 2.23±0.07Aa |
The long variance analysis table of table 19 sand culture test root
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| Between district's group | 0.1442 | 3 | 0.0481 | 0.885 | 0.4567 |
| Between process | 62.3021 | 14 | 4.4501 | 81.924 | 0 |
| Error | 2.2815 | 42 | 0.0543 | ||
| Total variation | 64.7278 | 59 |
The long variance analysis table of table 20 Drought resistant experiment root
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| Between district's group | 0.2827 | 3 | 0.0942 | 2.379 | 0.0833 |
| Between process | 15.5996 | 14 | 1.1143 | 28.129 | 0 |
| Error | 1.6637 | 42 | 0.0396 | ||
| Total variation | 17.546 | 59 |
The long variance analysis table of table 21 Salt tolerance root
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| Between district's group | 0.0461 | 3 | 0.0154 | 0.72 | 0.5457 |
| Between process | 7.0375 | 14 | 0.5027 | 23.53 | 0 |
| Error | 0.8973 | 42 | 0.0214 | ||
| Total variation | 7.9809 | 59 |
Table 22 Insect resistance assay rate of damage variance analysis table
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| Between district's group | 0.0042 | 3 | 0.0014 | 0.658 | 0.5825 |
| Between process | 6.0204 | 14 | 0.43 | 203.17 | 0 |
| Error | 0.0889 | 42 | 0.0021 | ||
| Total variation | 6.1135 | 59 |
Claims (10)
1. a compound wheat seed soaking agent, is characterized in that, is grouped into by following one-tenth: uniconazole P, Bachmedesh, diphenylurea and acetic acid.
2. according to compound wheat seed soaking agent according to claim 1, it is characterized in that, by mass percentage, the consumption of each composition is: uniconazole P 0.4%-0.5%, Bachmedesh 1.2%-1.5%, diphenylurea 0.5%-0.6%, surplus are acetic acid; Preferably, the consumption of each composition is: uniconazole P 0.5%, Bachmedesh 1.5%, diphenylurea 0.5%; Described acetic acid is preferably the acetic acid of content >=75%.
3. prepare a method for compound wheat seed soaking agent described in claim 1 or 2, it is characterized in that, the method comprises: join in acetic acid by uniconazole P, Bachmedesh and diphenylurea, stir, to obtain final product.
4. a compound wheat seed soaking agent, is characterized in that: be made up of composition I and composition II; Wherein, described composition I is made up of uniconazole P, Bachmedesh, diphenylurea and acetic acid, and described composition II is zasiokaurin liquid; Preferably, described acetic acid is preferably the acetic acid of content >=75%.
5. according to compound wheat seed soaking agent according to claim 4, it is characterized in that: by mass percentage, in composition I, the consumption of each composition is: uniconazole P 0.4%-0.5%, Bachmedesh 1.2%-1.5%, diphenylurea 0.5%-0.6%, surplus are acetic acid; Preferably, the consumption of each composition is: uniconazole P 0.5%, Bachmedesh 1.5%, diphenylurea 0.5%;
According to mass ratio meter, composition I: composition II=1:5000-15000; Preferably, composition I: composition II=1:7500-12500; Most preferred, composition I: composition II=1:10000.
6. according to compound wheat seed soaking agent according to claim 4, it is characterized in that: described zasiokaurin liquid obtains after zasiokaurin stoste dilute with water;
Preferably, described zasiokaurin stoste prepares in accordance with the following methods:
(1) zasiokaurin is gathered;
(2) deposit freezing at the temperature of-10 DEG C ~-20 DEG C for zasiokaurin;
(3) joined in hot water by the zasiokaurin after freezing depositing and stir, cooling, to obtain final product.
7. according to compound wheat seed soaking agent according to claim 6, it is characterized in that: described zasiokaurin liquid obtains after zasiokaurin stoste dilute with water 2-20 times; Preferably, described zasiokaurin liquid obtains after zasiokaurin stoste dilute with water 2-8 times; Most preferred, described zasiokaurin liquid obtains after zasiokaurin stoste dilute with water 5 times;
Described zasiokaurin stoste prepares in accordance with the following methods:
(1) gather zasiokaurin when corn full-bloom stage, be exposed to the sun the zasiokaurin that the same day gathers 1-2 hour in the sun;
(2) deposit freezing at the temperature of-20 DEG C for the zasiokaurin after being exposed to the sun;
(3) the freezing zasiokaurin deposited at least 30 days is joined in 80-100 DEG C of hot water of 3-20 times of quality and stir, after cooling and get final product; Preferably, the freezing zasiokaurin deposited at least 30 days is joined in 100 DEG C of hot water of 10 times of quality.
8. prepare a method for the compound wheat seed soaking agent described in claim 4 or 5, it is characterized in that, comprising: composition I and composition II are mixed, to obtain final product.
9. claim 1-3 compound wheat seed soaking agent described in any one reducing Plant Height in Wheat, short root urgees to strengthen, increase lodging resistance or improve the application in resistance; Preferably, described application comprises: by described compound wheat seed soaking agent dilute with water, obtains the compound wheat seed soaking agent after diluting; Compound wheat seed soaking agent after being diluted by wheat seed is soaked seed; Wherein, according to mass ratio meter, the ratio of wheat seed and the compound wheat seed soaking agent liquid after diluting is preferably 1:3; Described immersion is preferably soaked seed 24 hours at 20 DEG C of-30 DEG C of temperature.
10. claim 4-7 compound wheat seed soaking agent described in any one reducing Plant Height in Wheat, short root urgees to strengthen, increase lodging resistance or improve the application in resistance; Preferably, described application comprises: soaked seed by the described compound wheat seed soaking agent of wheat seed; Wherein, according to mass ratio meter, the ratio of wheat seed and compound wheat seed soaking agent is preferably 1:3; Described immersion is preferably soaked seed 24 hours at 20 DEG C of-30 DEG C of temperature.
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| CN107183059A (en) * | 2016-03-15 | 2017-09-22 | 陕西韦尔奇作物保护有限公司 | A kind of plant growth regualting composition of the ester containing chrysanthemin |
| CN107197875A (en) * | 2016-03-17 | 2017-09-26 | 陕西韦尔奇作物保护有限公司 | A kind of compound botanical growth regulating composition of the ester containing chrysanthemin |
| CN107197865A (en) * | 2016-03-18 | 2017-09-26 | 陕西韦尔奇作物保护有限公司 | A kind of volume increase agricultural chemicals of the ester containing chrysanthemin |
| CN107223664A (en) * | 2016-03-25 | 2017-10-03 | 陕西韦尔奇作物保护有限公司 | A kind of composition containing DCPTA |
| CN106818873A (en) * | 2016-12-27 | 2017-06-13 | 唐林元 | A kind of preparation method for improving wheat frost resistance spacetabs type seed soak |
| CN110256149A (en) * | 2019-06-28 | 2019-09-20 | 三峡大学 | A kind of activating additive and its preparation method and application suitable for planting unboiled water soil restoration of the ecosystem substrate |
| CN110226504A (en) * | 2019-07-12 | 2019-09-13 | 山西省农业科学院农作物品种资源研究所 | A kind of cultural method promoting Resistance of Wheat To Adversity |
| CN110226473A (en) * | 2019-07-12 | 2019-09-13 | 山西省农业科学院农作物品种资源研究所 | A kind of implantation methods of High-yield Wheat |
| CN110226473B (en) * | 2019-07-12 | 2021-09-07 | 山西省农业科学院农作物品种资源研究所 | High-yield wheat planting method |
| CN110226504B (en) * | 2019-07-12 | 2021-09-07 | 山西省农业科学院农作物品种资源研究所 | Cultivation method for improving stress resistance of wheat |
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