CN103030466B - Dendrobium nutrient solution formula for facilitating fast growth and high quality of dendrobium transplanting test-tube plantlets and application of dendrobium nutrient solution formula - Google Patents
Dendrobium nutrient solution formula for facilitating fast growth and high quality of dendrobium transplanting test-tube plantlets and application of dendrobium nutrient solution formula Download PDFInfo
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- 230000012010 growth Effects 0.000 title abstract description 16
- 241001523681 Dendrobium Species 0.000 title abstract description 13
- 235000015097 nutrients Nutrition 0.000 title abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 23
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims abstract description 12
- 230000003203 everyday effect Effects 0.000 claims abstract description 12
- 238000002791 soaking Methods 0.000 claims abstract description 3
- 240000004638 Dendrobium nobile Species 0.000 claims description 53
- 230000000050 nutritive effect Effects 0.000 claims description 39
- 239000007921 spray Substances 0.000 claims description 29
- 235000016709 nutrition Nutrition 0.000 claims description 14
- 238000005507 spraying Methods 0.000 claims description 13
- 238000007654 immersion Methods 0.000 claims description 12
- 239000011159 matrix material Substances 0.000 claims description 12
- 230000035764 nutrition Effects 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 11
- 230000003442 weekly effect Effects 0.000 claims description 8
- 239000012895 dilution Substances 0.000 claims description 6
- 238000010790 dilution Methods 0.000 claims description 6
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 4
- 239000002253 acid Substances 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 230000007935 neutral effect Effects 0.000 claims description 3
- 229910019142 PO4 Inorganic materials 0.000 claims description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 2
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 2
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Inorganic materials [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 claims description 2
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 claims description 2
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 2
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 2
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 2
- 239000010452 phosphate Substances 0.000 claims description 2
- 239000011591 potassium Substances 0.000 claims description 2
- 229910052700 potassium Inorganic materials 0.000 claims description 2
- 239000011573 trace mineral Substances 0.000 claims description 2
- 235000013619 trace mineral Nutrition 0.000 claims description 2
- 230000004083 survival effect Effects 0.000 abstract description 6
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 abstract 2
- 102000018997 Growth Hormone Human genes 0.000 abstract 1
- 108010051696 Growth Hormone Proteins 0.000 abstract 1
- 239000003242 anti bacterial agent Substances 0.000 abstract 1
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 abstract 1
- 239000006013 carbendazim Substances 0.000 abstract 1
- 239000000122 growth hormone Substances 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 201000010099 disease Diseases 0.000 description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 7
- 241000196324 Embryophyta Species 0.000 description 6
- 241000607479 Yersinia pestis Species 0.000 description 6
- 210000003608 fece Anatomy 0.000 description 5
- 239000010871 livestock manure Substances 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 3
- 230000008635 plant growth Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 241001076416 Dendrobium tosaense Species 0.000 description 2
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- 241001465754 Metazoa Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- -1 every day Substances 0.000 description 1
- 239000012092 media component Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000019786 weight gain Nutrition 0.000 description 1
- 239000004563 wettable powder Substances 0.000 description 1
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Abstract
The invention discloses a dendrobium nutrient solution formula for facilitating fast growth and high quality of dendrobium transplanting test-tube plantlets and an application of the dendrobium nutrient solution formula. A nutrient solution comprises the following components by volume percentage: 70-75% of Knudson C culture medium macroelements, 8-15% of microelements, 0.75-1.25% of 200mg/ml growth hormone NAA (naphthalene acetic acid) and 15-20% of carbendazim antibacterial agent with a mass fraction of 0.6-0.7%. Root-soaking treatment is conducted on the test-tube plantlets before transplanting, water is sprayed for once every day after the test-tube plantlets are transplanted, and the dendrobium nutrient solution is sprayed for once every week, so that the fast growth and the high quality of the cultivating plantlets are facilitated. The two problems of low transplanting survival rate and slow growth of the test-tube plantlets are solved, therefore, not only is the transplanting survival rate of the dendrobium test-tube plantlets increased, but also the fast growth, high yield, stable yield and high quality of the dendrobium cultivating plantlets are facilitated; and a technical support is provided for establishing an artificial large-area dendrobium cultivating base and achieving industrial dendrobium production.
Description
Technical field
The present invention relates to plant growing field, be specifically related to promote the stem of noble dendrobium to transplant stem of noble dendrobium nutrient solution prescription and the application thereof of test-tube plantlet fast-growing high-quality.
Background technology
The stem of noble dendrobium is the orchid family Dendrobium Sw, is a kind of conventional rare Chinese medicine.Approximately there are 76 kinds of Dendrobium Sws in China, and being made at present medicinal former plant is 45 kinds.Because of the development of medicinal demand, wild Dendrodium resource is on the brink of extinction, and setting up a kind of efficient artificial culture production technology is the current essential important topic solving.Stem of noble dendrobium tissue culture quick breeding seeding raising technology is already ripe, and the problem mainly facing is two hang-ups that test-tube plantlet bottle outlet transplanting survival rate is low and growth is slow.At present, although seedling age, transplanting time, transplanting condition to stem of noble dendrobium test-tube seedling transplanting had research, still lower and poor growth of test-tube plantlet bottle outlet transplanting survival rate.The development of stem of noble dendrobium nutrient solution prescription and application thereof, promote cultivation seedling fast-growing high-quality.
Summary of the invention
The object of this invention is to provide a kind of method of stem of noble dendrobium test-tube plantlet fast-growing that promotes that plant tissue culture is transplanted, solve the low and poky difficult problem of test-tube seedling transplanting surviving rate.
It is as follows that the present invention realizes goal of the invention technical scheme:
Promote the stem of noble dendrobium to transplant a nutritive medium for test-tube plantlet fast-growing, its feature is, this nutritive medium contains following component with volume ratio: Knudson C substratum macroelement 70-75%, micro-8-15%; The derosal antiseptic-germicide 15-20% of 200mg/mL tethelin NAA 0.75-1.25%, massfraction 0.6-0.7%.
Described Knudson C substratum macroelement consists of: liquid containing ammonium sulfate 500mg, potassium primary phosphate 250mg, magnesium sulfate 200mg, four water-calcium nitrate 1000mg in 1L nutritive medium; Described trace element consists of: in 1L nutritive medium, contain four water manganous sulfate 7.5mg, iron vitriol 25mg.
As preferably, the proportioning of described tethelin NAA is 1.0%.
Promote the stem of noble dendrobium to transplant a method for test-tube plantlet fast-growing, comprise the following steps:
(1) through the test-tube plantlet of tissue culture, before transplanting, natural light closed after bottle exercise 2-3 week, took out and cleaned, and used stem of noble dendrobium nutrition immersion root to process 1-5 hour; This step nutritive medium is 10 times of acquisitions of above-mentioned nutritive medium dilution;
(2) by soaking the test-tube plantlet of root processing in step (1), transplant to matrix and grow, every day, spray water 1-2 time, sprayed weekly stem of noble dendrobium nutritive medium 1 time, and the each amount of spraying of described spray water is 10-15ml/ strain, and the described stem of noble dendrobium nutritive medium at every turn amount of spraying is 5ml/ strain; This step nutritive medium is 1000 times of acquisitions of above-mentioned nutritive medium dilution.
As preferably, in described step (1), the stem of noble dendrobium nutrition immersion root treatment time is 2 hours; Matrix in described step (2) is neutral or acid sandstone.
Compared with the prior art, its beneficial effect is embodied in the present invention:
(1) in stem of noble dendrobium nutritive medium of the present invention, contain for the required various elements of stem of noble dendrobium test-tube plantlet fast-growing, its balanced nutritive ingredient can not only promote the Fast Growth of dendrobe cultivation seedling, and has improved the quality of dendrobe cultivation seedling.
(2) the present invention uses the nutritive medium of different concns to soak after root is processed and transplanted and spray processing transplanting before test-tube plantlet is transplanted, before wherein transplanting, soak root processing and can not only provide available nutrient for transplanting test-tube plantlet, and can effectively prevent the disease and pest of test-tube seedling transplanting back root part, improve transplanting survival rate; After transplanting, repeatedly spray processing, can ensure on the one hand the stem of noble dendrobium transplanted seedling required nutrient of growing, and can effectively prevent and treat the stem of stem of noble dendrobium transplanted seedling, leaf disease insect pest.
Embodiment
Below preferred embodiment the present invention is described further by following.
1, stem of noble dendrobium compounding:
Prepare by following volume ratio: Knudson C substratum macroelement 70-75%, micro-8-15%; Its optimum ratio of 200mg/mL tethelin NAA 0.75-1.25%(is 1.0%), derosal antiseptic-germicide 15-20%.Wherein, the wettable powder derosal antiseptic-germicide that derosal antiseptic-germicide is produced purchased from Tianjin Baisheng Chemical Co., Ltd., its chemical composition is derosal (N-(2-benzimidazolyl base)-Urethylane); Knudson C nutrient media components is as shown in table 1;
The stem of noble dendrobium nutritive medium that soaks root processing for transplanting test-tube plantlet bottle outlet is by 10 times of nutritive medium dilutions above, and the stem of noble dendrobium nutritive medium spraying is by 1000 times of nutritive medium dilutions above.
2, stem of noble dendrobium test-tube seedling transplanting
(1) in Best Times 4, the May of annual stem of noble dendrobium test-tube seedling transplanting, choose the healthy and strong stem of noble dendrobium test-tube plantlet of the high about 4-5 ㎝ of stem of tissue culture, tame hardening.First test-tube plantlet is moved to natural light place and close bottle exercise 2-3 week, carefully take out test-tube plantlet and clean, use stem of noble dendrobium nutrition immersion root to process 1-5 hour (Best Times 2 hours).
(2) will in step (1), soak the test-tube plantlet of root processing, transplant to neutral or acid sandstone matrix and grow in clump cultivation mode, in the moistening ventilative environment in cool place, every day, spray water 1-2 time, sprayed weekly stem of noble dendrobium nutritive medium 1 time; The each amount of spraying of spray water is 10-15ml/ strain, and the stem of noble dendrobium nutritive medium at every turn amount of spraying is 5ml/ strain.
Embodiment 1:
Choosing Herba Dendrobii test-tube plantlet and dendrobium officinale test-tube plantlet is example, experimental group: transplant front with the processing of stem of noble dendrobium nutrition immersion root, transplant to matrix, every day spray water 1-2 time, spray weekly stem of noble dendrobium nutritive medium 1 time, the each amount of spraying of spray water is 10-15ml/ strain, and the stem of noble dendrobium nutritive medium at every turn amount of spraying is 5ml/ strain; Control group: without the processing of stem of noble dendrobium nutrition immersion root, transplant to matrix and only spray water or spray farm manure for contrast before transplanting.The control group of wherein spraying water is to spray water 1-2 time every day, each 10-15 mL/ strain; Spray farm manure control group is to spray water 1-2 time every day, and each 10-15 mL/ strain, sprays weekly farm manure 1 time, each 5-10mL/ strain, and farm manure is mainly for people, domestic animal mix just liquid.Other cultivation condition of experimental group and control group is identical.Result as shown in table 2, table 3, experimental group cultivation seedling, upgrowth situation is good, plant year rate of body weight gain reach more than 90%; And the control group that sprays farm manure is cultivated seedling, because nutritive ingredient is unbalanced, and content of microorganisms is many, easily grows disease and pest, causes rotten; The control group cultivation seedling that only sprays water, growing way is general, and easily grows disease and pest.
The impact of table 2 nutritional condition on the growth of Herba Dendrobii test-tube seedling transplanting
Note: in above-mentioned table, the rear Plant Leaf look of cultivation is light green, the generous stretching, extension of blade, and cane is sturdy, well developed root system, what growth potential was strong is to grow in order; After planting, Plant Leaf look green, blade is wider, stretching, extension, and cane is thicker, and what root system was more flourishing is that upgrowth situation is general; After planting, Plant Leaf look yellowish green, blade face is narrow, and cane is thin and delicate, and what growth potential was weak is that upgrowth situation is poor.Identical in following table.
Table 3 nutritional condition is transplanted the impact of growth on dendrobium officinale test-tube plantlet
Embodiment 2
Choosing Herba Dendrobii test-tube plantlet is example, and the stem of noble dendrobium test-tube seedling transplanting of experimental group soaks root to be processed 2 hours, before the stem of noble dendrobium test-tube seedling transplanting of control group, does not do and soaks root processing; Transplant to after matrix all only to spray water as contrast, spray water 1-2 every day, each 10-15 mL; Other cultivation condition is identical.The plant growth condition of result stem of noble dendrobium nutrition as shown in table 4 immersion root processing does not more soak the good of root processing; And the plant disease and pest of experimental group is obviously less than the disease and pest of control group, especially root, the root of experimental group is disease-free.
Table 4 soaks root and processes the impact on the growth of Herba Dendrobii test-tube seedling transplanting
Note: in table, upgrowth situation is better, after representing to plant plant strain growth gesture between above-mentioned good with generally between.Identical in following table.
Embodiment 3
Choosing Herba Dendrobii test-tube plantlet is example, experimental group: before transplanting, process 2 hours with stem of noble dendrobium nutrition immersion root, transplant to matrix, every day, spray water 1-2 time, sprayed weekly stem of noble dendrobium nutritive medium 1 time; The each amount of spraying of spray water is 10-15ml/ strain, and the stem of noble dendrobium nutritive medium at every turn amount of spraying is 5ml/ strain; Control group: before transplanting, process 2 hours with stem of noble dendrobium nutrition immersion root, transplant to matrix, every day only spray water 1-2 time, each amount of spraying is 10-15ml/ strain; Other cultivation condition is identical.Result is as shown in table 5, and the plant growth condition of experimental group is good compared with the indices of control group.
Table 5 sprays the impact of nutritive medium on the growth of Herba Dendrobii test-tube seedling transplanting
Embodiment 4
Choosing Herba Dendrobii test-tube plantlet is example, experimental group: before transplanting, with the stem of noble dendrobium nutrition immersion root processing of adding derosal antiseptic-germicide, transplant to matrix, every day spray water 1-2 time, the stem of noble dendrobium nutritive medium 1 time of spray interpolation derosal antiseptic-germicide weekly, consumption is the same; Control group: before transplanting, with not adding the stem of noble dendrobium nutrition immersion root processing of derosal antiseptic-germicide, transplant to matrix, every day spray water 1-2 time, the stem of noble dendrobium nutritive medium 1 time of spray interpolation derosal antiseptic-germicide weekly, consumption is the same.Result is as shown in table 6, and the plant growth condition of experimental group is good, and transplanting survival rate is high, and the plant of control group, the due to illness worm harm of root and cauline leaf, causes surviving rate lower.
The impact of table 6 derosal antiseptic-germicide on the growth of Herba Dendrobii test-tube seedling transplanting
Claims (6)
1. promote the stem of noble dendrobium to transplant a nutritive medium for test-tube plantlet fast-growing high-quality, it is characterized in that, this nutritive medium contains following proportioning component with volume ratio: Knudson C substratum macroelement 70-75%, micro-8-15%; The derosal antiseptic-germicide 15-20% of 200mg/mL tethelin NAA 0.75-1.25%, massfraction 0.6-0.7%.
2. a kind of nutritive medium that promotes stem of noble dendrobium transplanting test-tube plantlet fast-growing high-quality according to claim 1, is characterized in that: the proportioning of described tethelin NAA is 1.0%.
3. a kind of nutritive medium that promotes stem of noble dendrobium transplanting test-tube plantlet fast-growing high-quality according to claim 1, it is characterized in that, described Knudson C substratum macroelement consists of: liquid containing ammonium sulfate 500mg, potassium primary phosphate 250mg, magnesium sulfate 200mg, four water-calcium nitrate 1000mg in 1L nutritive medium; Described trace element consists of: in 1L nutritive medium, contain four water manganous sulfate 7.5mg, iron vitriol 25mg.
4. promote the stem of noble dendrobium to transplant a method for test-tube plantlet fast-growing high-quality, it is characterized in that, comprise the following steps:
(1) through the test-tube plantlet of tissue culture, before transplanting, natural light closed after bottle exercise 2-3 week, took out and cleaned, and used stem of noble dendrobium nutrition immersion root to process 1-5 hour; This step nutritive medium is 10 times of acquisitions of nutritive medium dilution described in claim 1;
(2) by soaking the test-tube plantlet of root processing in step (1), transplant to matrix and grow, every day, spray water 1-2 time, sprayed weekly stem of noble dendrobium nutritive medium 1 time, and the each amount of spraying of described spray water is 10-15ml/ strain, and the described stem of noble dendrobium nutritive medium at every turn amount of spraying is 5ml/ strain; This step nutritive medium is 1000 times of acquisitions of nutritive medium dilution described in claim 1.
5. a kind of method that promotes stem of noble dendrobium transplanting test-tube plantlet fast-growing high-quality according to claim 4, is characterized in that, in described step (1), the stem of noble dendrobium nutrition immersion root treatment time is 2 hours.
6. a kind of method that promotes stem of noble dendrobium transplanting test-tube plantlet fast-growing high-quality according to claim 4, is characterized in that, the matrix in described step (2) is neutral or acid sandstone.
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| CN103782886B (en) * | 2014-02-13 | 2015-12-30 | 云南新维特生物科技有限公司 | A kind of greenhouse gardening method of dendrobium candidum and application |
| CN103918536B (en) * | 2014-04-30 | 2016-01-13 | 成都润惠农业开发有限公司 | The implantation methods of dendrobium candidum |
| CN104370646A (en) * | 2014-11-13 | 2015-02-25 | 柳州市天姿园艺有限公司 | Nutrient solution capable of preventing and treating black spot of Dendrobium officinale |
| CN104478553A (en) * | 2014-11-17 | 2015-04-01 | 柳州市天姿园艺有限公司 | Nutrient solution for control of dendrobium officinale sooty mold |
| CN104429868B (en) * | 2014-12-27 | 2017-01-11 | 福建农林大学 | Method for cultivating dendrobium candidum and anoectochilus roxburghii through rock in forest land |
| CN106083382A (en) * | 2016-06-23 | 2016-11-09 | 松桃如阿雅观光农业产业开发有限公司 | A kind of Fructus Citri grandis nursery agent |
| CN107771658A (en) * | 2016-08-27 | 2018-03-09 | 杜名胜 | A kind of method for culturing seedlings for improving Dendrobidium huoshanness transplanting survival rate |
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| CN101653096A (en) * | 2008-08-20 | 2010-02-24 | 汉中植物研究所 | Method for tissue-culture breeding and wild-simulated cultivation of dendrobium candidum |
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Effective date of registration: 20200805 Address after: 530031 business place of agricultural and sideline products wholesale market in Suwei Town, Jiangnan District, Nanning City, Guangxi Zhuang Autonomous Region Patentee after: Guangxi nongfengshan Dendrobium officinale Technology Co., Ltd Address before: No.2501, unit 2, shangshangyuan, no.708-1, west side of Yinhai Avenue, Liangqing District, Nanning City, Guangxi Zhuang Autonomous Region Patentee before: Guangxi zhuoshang Agricultural Technology Co., Ltd |
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