CN103030466A - Dendrobium nutrient solution formula for facilitating fast growth and high quality of dendrobium transplanting test-tube plantlets and application of dendrobium nutrient solution formula - Google Patents
Dendrobium nutrient solution formula for facilitating fast growth and high quality of dendrobium transplanting test-tube plantlets and application of dendrobium nutrient solution formula Download PDFInfo
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- 230000012010 growth Effects 0.000 title abstract description 15
- 241001523681 Dendrobium Species 0.000 title abstract description 13
- 235000015097 nutrients Nutrition 0.000 title abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 23
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims abstract description 12
- 230000003203 everyday effect Effects 0.000 claims abstract description 12
- 238000002791 soaking Methods 0.000 claims abstract description 4
- 240000004638 Dendrobium nobile Species 0.000 claims description 53
- 230000000050 nutritive effect Effects 0.000 claims description 51
- 239000007921 spray Substances 0.000 claims description 29
- 238000000034 method Methods 0.000 claims description 13
- 239000011159 matrix material Substances 0.000 claims description 12
- 238000005507 spraying Methods 0.000 claims description 12
- 230000003442 weekly effect Effects 0.000 claims description 8
- 239000012895 dilution Substances 0.000 claims description 6
- 238000010790 dilution Methods 0.000 claims description 6
- 239000002253 acid Substances 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 230000007935 neutral effect Effects 0.000 claims description 3
- 229910019142 PO4 Inorganic materials 0.000 claims description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 2
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 2
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Inorganic materials [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 claims description 2
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 claims description 2
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 2
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 2
- 239000010452 phosphate Substances 0.000 claims description 2
- 239000011591 potassium Substances 0.000 claims description 2
- 229910052700 potassium Inorganic materials 0.000 claims description 2
- 239000011573 trace mineral Substances 0.000 claims description 2
- 235000013619 trace mineral Nutrition 0.000 claims description 2
- 230000004083 survival effect Effects 0.000 abstract description 6
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 abstract 2
- 102000018997 Growth Hormone Human genes 0.000 abstract 1
- 108010051696 Growth Hormone Proteins 0.000 abstract 1
- 239000003242 anti bacterial agent Substances 0.000 abstract 1
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 abstract 1
- 239000006013 carbendazim Substances 0.000 abstract 1
- 239000000122 growth hormone Substances 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 201000010099 disease Diseases 0.000 description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 7
- 241000196324 Embryophyta Species 0.000 description 6
- 241000607479 Yersinia pestis Species 0.000 description 6
- 210000003608 fece Anatomy 0.000 description 5
- 239000010871 livestock manure Substances 0.000 description 5
- -1 every day Substances 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 230000008635 plant growth Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 241001076416 Dendrobium tosaense Species 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000012092 media component Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000019786 weight gain Nutrition 0.000 description 1
- 239000004563 wettable powder Substances 0.000 description 1
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Abstract
The invention discloses a dendrobium nutrient solution formula for facilitating fast growth and high quality of dendrobium transplanting test-tube plantlets and an application of the dendrobium nutrient solution formula. A nutrient solution comprises the following components by volume percentage: 70-75% of Knudson C culture medium macroelements, 8-15% of microelements, 0.75-1.25% of 200mg/ml growth hormone NAA (naphthalene acetic acid) and 15-20% of carbendazim antibacterial agent with a mass fraction of 0.6-0.7%. Root-soaking treatment is conducted on the test-tube plantlets before transplanting, water is sprayed for once every day after the test-tube plantlets are transplanted, and the dendrobium nutrient solution is sprayed for once every week, so that the fast growth and the high quality of the cultivating plantlets are facilitated. The two problems of low transplanting survival rate and slow growth of the test-tube plantlets are solved, therefore, not only is the transplanting survival rate of the dendrobium test-tube plantlets increased, but also the fast growth, high yield, stable yield and high quality of the dendrobium cultivating plantlets are facilitated; and a technical support is provided for establishing an artificial large-area dendrobium cultivating base and achieving industrial dendrobium production.
Description
Technical field
The present invention relates to the plant growing field, be specifically related to promote the stem of noble dendrobium to transplant stem of noble dendrobium nutrient solution prescription and the application thereof of test-tube plantlet fast-growing high-quality.
Background technology
The stem of noble dendrobium is the orchid family Dendrobium Sw, is a kind of rare Chinese medicine commonly used.Approximately there are 76 kinds of Dendrobium Sws in China, and being made at present medicinal former plant is 45 kinds.Because of the development of medicinal demand, the wild Dendrodium resource is on the brink of extinction, and setting up a kind of efficient artificial culture production technology is the current essential important topic that solves.Stem of noble dendrobium tissue culture quick breeding seeding raising technology is already ripe, and the problem that mainly faces is the low and two slow hang-ups of growing of test-tube plantlet bottle outlet transplanting survival rate.At present, although seedling age, transplanting time, the transplanting condition of stem of noble dendrobium test-tube seedling transplanting had research, test-tube plantlet bottle outlet transplanting survival rate is lower and poor growth still.The development of stem of noble dendrobium nutrient solution prescription and application thereof promote cultivation seedling fast-growing high-quality.
Summary of the invention
The purpose of this invention is to provide a kind of method that promotes the stem of noble dendrobium test-tube plantlet fast-growing that plant tissue culture is transplanted, solve the test-tube seedling transplanting surviving rate and hang down and a poky difficult problem.
The present invention realizes that the goal of the invention technical scheme is as follows:
A kind of stem of noble dendrobium that promotes is transplanted the nutritive medium of test-tube plantlet fast-growing, and its characteristics are, this nutritive medium contains following component with volume ratio: Knudson C substratum macroelement 70-75%, micro-8-15%; The derosal antiseptic-germicide 15-20% of 200mg/mL tethelin NAA 0.75-1.25%, massfraction 0.6-0.7%.
Described Knudson C substratum macroelement consists of: liquid containing ammonium sulfate 500mg, potassium primary phosphate 250mg, sal epsom 200mg, four water-calcium nitrate 1000mg in the 1L nutritive medium; Described trace element consists of: contain four water manganous sulfate 7.5mg, iron vitriol 25mg in the 1L nutritive medium.
As preferably, the proportioning of described tethelin NAA is 1.0%.
A kind of stem of noble dendrobium that promotes is transplanted the method for test-tube plantlet fast-growing, may further comprise the steps:
(1) through the test-tube plantlet of tissue culture, natural light closes bottle and tempers 2-3 after week before transplanting, and takes out and cleans, and soaks root with stem of noble dendrobium nutritive medium and processes 1-5 hour; This step nutritive medium is 10 times of acquisitions of above-mentioned nutritive medium dilution;
(2) with soaking the test-tube plantlet of root processing in the step (1), transplant to matrix and grow, every day, spray water was 1-2 time, sprayed weekly stem of noble dendrobium nutritive medium 1 time, and the each amount of spraying of described spray water is the 10-15ml/ strain, and the described stem of noble dendrobium nutritive medium at every turn amount of spraying is the 5ml/ strain; This step nutritive medium is 1000 times of acquisitions of above-mentioned nutritive medium dilution.
As preferably, to soak the root treatment time be 2 hours to stem of noble dendrobium nutritive medium in the described step (1); Matrix in the described step (2) is neutral or acid sandstone.
The present invention compared with the prior art, its beneficial effect is embodied in:
(1) contain in the stem of noble dendrobium nutritive medium of the present invention for the required various elements of stem of noble dendrobium test-tube plantlet fast-growing, its balanced nutritive ingredient can not only promote the Fast Growth of dendrobe cultivation seedling, and has improved the quality of dendrobe cultivation seedling.
(2) the present invention uses the nutritive medium of different concns to spray processing to transplanting to soak before test-tube plantlet is transplanted after root is processed and transplanted, soaking root before wherein transplanting processes and can not only provide available nutrient for transplanting test-tube plantlet, and can effectively prevent the disease and pest of test-tube seedling transplanting back root part, improve transplanting survival rate; Repeatedly spray processing after the transplanting, can guarantee on the one hand the stem of noble dendrobium transplanted seedling required nutrient of growing, and can stem, the leaf disease insect pest of stem of noble dendrobium transplanted seedling effectively be prevented and treated.
Embodiment
Below preferred embodiment the present invention is described further by following.
1, stem of noble dendrobium compounding:
Prepare by following volume ratio: Knudson C substratum macroelement 70-75%, micro-8-15%; Its optimum ratio of 200mg/mL tethelin NAA 0.75-1.25%(is 1.0%), derosal antiseptic-germicide 15-20%.Wherein, the wettable powder derosal antiseptic-germicide that the derosal antiseptic-germicide is produced available from Tianjin Baisheng Chemical Co., Ltd., its chemical composition is derosal (N-(2-benzimidazolyl base)-Urethylane); Knudson C nutrient media components is as shown in table 1;
The stem of noble dendrobium nutritive medium that soaks the root processing for transplanting test-tube plantlet bottle outlet is with 10 times of top nutritive medium dilutions, and the stem of noble dendrobium nutritive medium that sprays is with 1000 times of top nutritive medium dilutions.
2, stem of noble dendrobium test-tube seedling transplanting
(1) in Best Times 4, the May of annual stem of noble dendrobium test-tube seedling transplanting, choose the healthy and strong stem of noble dendrobium test-tube plantlet of the high approximately 4-5 ㎝ of stem of tissue culture, tame hardening.First test-tube plantlet is moved to the natural light place and close bottle exercise 2-3 week, carefully take out test-tube plantlet and clean, soak root with stem of noble dendrobium nutritive medium and process 1-5 hour (Best Times 2 hours).
(2) will soak the test-tube plantlet of root processing in the step (1), transplant to neutral or acid sandstone matrix in clump cultivation mode and grow, in the moistening ventilative environment in cool place, every day, spray water was 1-2 time, sprayed weekly stem of noble dendrobium nutritive medium 1 time; The each amount of spraying of spray water is the 10-15ml/ strain, and the stem of noble dendrobium nutritive medium at every turn amount of spraying is the 5ml/ strain.
Embodiment 1:
Choosing Herba Dendrobii test-tube plantlet and dendrobium officinale test-tube plantlet is example, experimental group: soak root with stem of noble dendrobium nutritive medium before transplanting and process, transplant to matrix, every day, spray water was 1-2 time, spray weekly stem of noble dendrobium nutritive medium 1 time, the each amount of spraying of spray water is the 10-15ml/ strain, and the stem of noble dendrobium nutritive medium at every turn amount of spraying is the 5ml/ strain; Control group: soaks root without stem of noble dendrobium nutritive medium before transplanting and process, transplanting only sprays water or spray farm manure to the matrix is contrast.The control group of wherein spraying water is to spray water 1-2 time every day, each 10-15 mL/ strain; Spray the farm manure control group is to spray water 1-2 time every day, and each 10-15 mL/ strain sprays weekly farm manure 1 time, each 5-10mL/ strain, and farm manure mainly mixes just liquid for people, domestic animal.Other cultivation condition of experimental group and control group is identical.The result shown in table 2, table 3, experimental group cultivation seedling, upgrowth situation is good, plant year rate of body weight gain reach more than 90%; And the control group that sprays farm manure is cultivated seedling, because nutritive ingredient is unbalanced, and content of microorganisms is many, easily grows disease and pest, causes mashed root; Only spray the control group cultivation seedling of water, growing way is general, and easily grows disease and pest.
Table 2 nutritional condition is to Herba Dendrobii test-tube seedling transplanting affects on the growth
Annotate: in the above-mentioned table, the rear Plant Leaf look of cultivation is light green, the generous stretching, extension of blade, and cane is sturdy, well developed root system, and what growth potential was strong is to grow in order; The Plant Leaf look green after planting, blade is wider, stretching, extension, and cane is thicker, and what root system was flourishing is that upgrowth situation is general; The Plant Leaf look yellowish green after planting, the blade face is narrow, and cane is thin and delicate, and what growth potential was weak is that upgrowth situation is poor.Identical in the following table.
Table 3 nutritional condition is transplanted affects on the growth to dendrobium officinale test-tube plantlet
Embodiment 2
Choosing the Herba Dendrobii test-tube plantlet is example, and the stem of noble dendrobium test-tube seedling transplanting of experimental group soaks root to be processed 2 hours, does not do before the stem of noble dendrobium test-tube seedling transplanting of control group and soaks the root processing; Transplant to the matrix all so that only water spray is as contrast, spray water 1-2 every day, at every turn 10-15 mL; Other cultivation condition is identical.The plant growth condition that result's stem of noble dendrobium nutritive medium as shown in table 4 soaks the root processing does not more soak the good of root processing; And the plant disease and pest of experimental group obviously is less than the disease and pest of control group, root especially, and the root of experimental group is disease-free.
Table 4 soaks root to be processed Herba Dendrobii test-tube seedling transplanting affects on the growth
Annotate: in the table, upgrowth situation is better, after expression is planted the plant strain growth gesture between above-mentioned good with generally between.Identical in the following table.
Embodiment 3
Choosing the Herba Dendrobii test-tube plantlet is example, experimental group: soak root with stem of noble dendrobium nutritive medium before transplanting and processed 2 hours, transplant to matrix, every day, spray water was 1-2 time, sprayed weekly stem of noble dendrobium nutritive medium 1 time; The each amount of spraying of spray water is the 10-15ml/ strain, and the stem of noble dendrobium nutritive medium at every turn amount of spraying is the 5ml/ strain; Control group: soaks root processing 2 hours with stem of noble dendrobium nutritive medium before transplanting, transplant to matrix, every day is spray water 1-2 time only, and each amount of spraying is the 10-15ml/ strain; Other cultivation condition is identical.The result is as shown in table 5, and the plant growth condition of experimental group is good than the indices of control group.
Table 5 spray nutritive medium is to Herba Dendrobii test-tube seedling transplanting affects on the growth
Embodiment 4
Choosing the Herba Dendrobii test-tube plantlet is example, experimental group: soak root with the stem of noble dendrobium nutritive medium that adds the derosal antiseptic-germicide before transplanting and process, transplant to matrix, every day, spray water was 1-2 time, sprayed weekly the stem of noble dendrobium nutritive medium 1 time of interpolation derosal antiseptic-germicide, and consumption is the same; Control group: soak root with the stem of noble dendrobium nutritive medium that does not add the derosal antiseptic-germicide before transplanting and process, transplant to matrix, every day, spray water was 1-2 time, the stem of noble dendrobium nutritive medium 1 time of spray interpolation derosal antiseptic-germicide weekly, and consumption is the same.The result is as shown in table 6, and the plant growth condition of experimental group is good, and transplanting survival rate is high, and the plant of control group, the due to illness worm harm of root and cauline leaf causes surviving rate lower.
Table 6 derosal antiseptic-germicide is to Herba Dendrobii test-tube seedling transplanting affects on the growth
Claims (6)
1. one kind promotes the stem of noble dendrobium to transplant the nutritive medium of test-tube plantlet fast-growing high-quality, it is characterized in that, this nutritive medium contains following proportioning component with volume ratio: Knudson C substratum macroelement 70-75%, micro-8-15%; The derosal antiseptic-germicide 15-20% of 200mg/mL tethelin NAA 0.75-1.25%, massfraction 0.6-0.7%.
2. a kind of stem of noble dendrobium that promotes according to claim 1 is transplanted the nutritive medium of test-tube plantlet fast-growing high-quality, and it is characterized in that: the proportioning of described tethelin NAA is 1.0%.
3. a kind of stem of noble dendrobium that promotes according to claim 1 is transplanted the nutritive medium of test-tube plantlet fast-growing high-quality, it is characterized in that, described Knudson C substratum macroelement consists of: liquid containing ammonium sulfate 500mg, potassium primary phosphate 250mg, sal epsom 200mg, four water-calcium nitrate 1000mg in the 1L nutritive medium; Described trace element consists of: contain four water manganous sulfate 7.5mg, iron vitriol 25mg in the 1L nutritive medium.
4. one kind promotes the stem of noble dendrobium to transplant the method for test-tube plantlet fast-growing high-quality, it is characterized in that, may further comprise the steps:
(1) through the test-tube plantlet of tissue culture, natural light closes bottle and tempers 2-3 after week before transplanting, and takes out and cleans, and soaks root with stem of noble dendrobium nutritive medium and processes 1-5 hour; This step nutritive medium is 10 times of acquisitions of the described nutritive medium dilution of claim 1;
(2) with soaking the test-tube plantlet of root processing in the step (1), transplant to matrix and grow, every day, spray water was 1-2 time, sprayed weekly stem of noble dendrobium nutritive medium 1 time, and the each amount of spraying of described spray water is the 10-15ml/ strain, and the described stem of noble dendrobium nutritive medium at every turn amount of spraying is the 5ml/ strain; This step nutritive medium is 1000 times of acquisitions of the described nutritive medium dilution of claim 1.
5. a kind of stem of noble dendrobium that promotes according to claim 4 is transplanted the method for test-tube plantlet fast-growing high-quality, it is characterized in that, it is 2 hours that the middle stem of noble dendrobium nutritive medium of described step (1) soaks the root treatment time.
6. a kind of stem of noble dendrobium that promotes according to claim 4 is transplanted the method for test-tube plantlet fast-growing high-quality, it is characterized in that, the matrix in the described step (2) is neutral or acid sandstone.
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Cited By (7)
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| CN103918536A (en) * | 2014-04-30 | 2014-07-16 | 成都润惠农业开发有限公司 | Planting method for Dendrobium officinale |
| CN104370646A (en) * | 2014-11-13 | 2015-02-25 | 柳州市天姿园艺有限公司 | Nutrient solution capable of preventing and treating black spot of Dendrobium officinale |
| CN104429868A (en) * | 2014-12-27 | 2015-03-25 | 福建农林大学 | Method for cultivating dendrobium candidum and anoectochilus roxburghii through rock in forest land |
| CN104478553A (en) * | 2014-11-17 | 2015-04-01 | 柳州市天姿园艺有限公司 | Nutrient solution for control of dendrobium officinale sooty mold |
| CN106083382A (en) * | 2016-06-23 | 2016-11-09 | 松桃如阿雅观光农业产业开发有限公司 | A kind of Fructus Citri grandis nursery agent |
| CN107771658A (en) * | 2016-08-27 | 2018-03-09 | 杜名胜 | A kind of method for culturing seedlings for improving Dendrobidium huoshanness transplanting survival rate |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103782886A (en) * | 2014-02-13 | 2014-05-14 | 云南新维特生物科技有限公司 | Greenhouse cultivating method and application of Dendrobium officinale |
| CN103782886B (en) * | 2014-02-13 | 2015-12-30 | 云南新维特生物科技有限公司 | A kind of greenhouse gardening method of dendrobium candidum and application |
| CN103918536A (en) * | 2014-04-30 | 2014-07-16 | 成都润惠农业开发有限公司 | Planting method for Dendrobium officinale |
| CN104370646A (en) * | 2014-11-13 | 2015-02-25 | 柳州市天姿园艺有限公司 | Nutrient solution capable of preventing and treating black spot of Dendrobium officinale |
| CN104478553A (en) * | 2014-11-17 | 2015-04-01 | 柳州市天姿园艺有限公司 | Nutrient solution for control of dendrobium officinale sooty mold |
| CN104429868A (en) * | 2014-12-27 | 2015-03-25 | 福建农林大学 | Method for cultivating dendrobium candidum and anoectochilus roxburghii through rock in forest land |
| CN106083382A (en) * | 2016-06-23 | 2016-11-09 | 松桃如阿雅观光农业产业开发有限公司 | A kind of Fructus Citri grandis nursery agent |
| CN107771658A (en) * | 2016-08-27 | 2018-03-09 | 杜名胜 | A kind of method for culturing seedlings for improving Dendrobidium huoshanness transplanting survival rate |
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