CN108739331A - A kind of cultural method of selenium-rich romaine lettuce - Google Patents
A kind of cultural method of selenium-rich romaine lettuce Download PDFInfo
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- CN108739331A CN108739331A CN201810771131.8A CN201810771131A CN108739331A CN 108739331 A CN108739331 A CN 108739331A CN 201810771131 A CN201810771131 A CN 201810771131A CN 108739331 A CN108739331 A CN 108739331A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/15—Leaf crops, e.g. lettuce or spinach
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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Abstract
The present invention provides a kind of cultural methods of selenium-rich romaine lettuce.The cultural method includes the following steps：1) it uses the first mill water culture nutrient solution to carry out water planting 7~14 days to romaine lettuce seedling, obtains romaine lettuce seedling；Se content is 0.1~0.2mg/L in first mill water culture nutrient solution；2) the second mill water culture nutrient solution is used to carry out water planting 7~21 days to the romaine lettuce seedling that step 1) obtains；Se content is 0.3~0.5mg/L in second mill water culture nutrient solution；3) water planting is carried out to the romaine lettuce seedling that step 2) obtains using first mill water culture nutrient solution.The cultural method of selenium-rich romaine lettuce provided by the invention carries out water planting selenium-rich to romaine lettuce, and selenium rich ability is stronger, while also reducing the pollution of pollution, heavy metal-polluted soil to vegetables to soil.
Description
Technical field
The present invention relates to water planting technical field of cultivation, more particularly, to a kind of cultural method of selenium-rich romaine lettuce.
Background technology
Selenium is minimum multifunctional life nutritional element necessary to a kind of human body and animal, by domestic and international the world of medicine and nutrition educational circles
It is respectfully called " kindling material of life ", there are the multi-efficiencies such as cancer-resisting, anti-oxidant and raising immunity.It is investigated according to expert, people needs
Lifelong selenium-supply is wanted, Diet cures more than the doctors.The researchs such as Finley also indicate that, feed Biological Selenium is most safely and effectively selenium-supply mode.
Necessity of the vegetables as diet, occupies very important status in resident's daily life.It is prior
It is that vegetables are converted into Organic Selenium and play a crucial role in nature selenium circular biological chain and inorganic selenium.Rich selenium vegetables
In selenium mainly exist in the form of Organic Selenium, physiological activity is high, is easily absorbed by the body.Therefore the selenium in vegetables is human nutrition
The main source of selenium directly influences the selemium nutrition situation of the mankind.Currently used vegetables selenium-rich method has：(1) soil spraying selenium,
By directly applying selenium fertilizer into soil, selenium is converted by plant roots and stems Absorption And Metabolism, improves the content of selenium in crop, but soil
Selenium meeting contaminated soil is applied, the requirement of ecological, environmental protective is unable to reach.(2) selenium is dressed seed, and Se-containing nutritive liquid or selenium fertilizer are applied to plant
On seed, and then the content of selenium in product is improved, but selenium amount specific leaf area used in the method sprays mostly 20 times or so, and selenium is dressed seed
To a certain extent can contaminated soil, the Se content in crop cannot quantify control in a certain range, food cannot be effectively ensured
With safety.(3) stalk injection selenium fertilizer, this method is excessively troublesome for agricultural production, can not promote.And water planting is as nothing
One kind in soil cultivation, with its yield is high, quality is good, water-saving saving of labor, no pollution, without the absolute pressures such as territory restriction, green safe
Sexual clorminance becomes the modernization vegetable growing technology of current most broad prospect of application.Research shows that：Vegetable aquaculture has selenium
Very high absorption rate, therefore be the effective way of human body selenium-supply using vegetable aquaculture selenium-rich, how to be effectively enriched with
The water planting romaine lettuce of machine selenium becomes the technical field and is badly in need of the technical barrier solved.
Invention content
The first object of the present invention is to provide a kind of cultural method of selenium-rich romaine lettuce, which includes following step
Suddenly：
1) it uses the first mill water culture nutrient solution to carry out water planting 7~14 days to romaine lettuce seedling, obtains romaine lettuce seedling；First water
Se content is 0.1~0.2mg/L in cultivation nutrient fluid；
2) the second mill water culture nutrient solution is used to carry out water planting 7~21 days to the romaine lettuce seedling that step 1) obtains；Second water
Se content is 0.3~0.5mg/L in cultivation nutrient fluid；
3) water planting is carried out to the romaine lettuce seedling that step 2) obtains using first mill water culture nutrient solution.
In a preferred embodiment of the invention, other than Se content is different, the first water planting nutrient solution and the second Aquaponic
Other components of liquid can be the same or different.Wherein, in parts by weight, the first water planting nutrient solution and/or the second water planting
Nutrient solution preferably further includes following component：0.2~1.0 calcium nitrate, 0.1~0.6 potassium nitrate, 0.05~0.3 ammonium dihydrogen phosphate,
0.1~0.7 magnesium sulfate, 0.01~0.03EDTA chelates, 0.002~0.006 boric acid, 0.002~0.004 manganese sulfate,
0.0002~0.0005 zinc sulfate, 0.00006~0.0001 copper sulphate, 0.00002~0.0001 ammonium molybdate and 1000 water.It is above-mentioned
The pH value of first water planting nutrient solution and the second water planting nutrient solution is 5.5~6.5.
Wherein, parts by weight can be the unit of weight well known in the art such as μ g, mg, g, kg in the present invention, can also be it
Multiple, such as 1/10,1/100,10 times, 100 times.
Wherein, in water planting nutrient solution selenium concentration by adjust into water planting nutrient solution be added selenium compound for example sodium selenite or
The amounts of DL- selenomethionines is adjusted.
Wherein, EDTA chelates preferably can be EDTA-Na or EDTA-Fe.
Wherein, further include following component in above-mentioned water planting nutrient solution：0.01~0.05 ferrous sulfate.
More preferably, in parts by weight, the first water planting nutrient solution and/or the second water planting nutrient solution further include it is following at
Point：0.2~0.3 calcium nitrate, 0.4~0.6 potassium nitrate, 0.05~0.06 ammonium dihydrogen phosphate, 0.1~0.3 magnesium sulfate, 0.01~
0.02 ferrous sulfate, 0.01~0.03 disodium ethylene diamine tetraacetate, 0.002~0.005 boric acid, 0.002~0.003 manganese sulfate,
0.0002~0.0005 zinc sulfate, 0.00006~0.0001 copper sulphate, 0.00002~0.00006 ammonium molybdate and 1000 water.
In a preferred embodiment of the invention, in parts by weight, the first water planting nutrient solution and/or the second Aquaponic
Liquid further includes following component：0.9~1.0 calcium nitrate, 0.2~0.5 potassium nitrate, 0.1~0.3 potassium dihydrogen phosphate, 0.5~0.7
Magnesium sulfate, 0.01~0.03EDTA-Fe, 0.003~0.006 boric acid, 0.002~0.004 manganese sulfate, 0.0002~0.0005 sulphur
Sour zinc, 0.00008~0.0001 copper sulphate, 0.00002~0.0001 ammonium molybdate and 1000 water.
Wherein, more preferably the first water planting nutrient solution and the second water planting nutrient solution in addition to selenium concentration Bu Tong other than, it is other at split-phase
Together, i.e., further include following component：0.2~0.3 calcium nitrate, 0.4~0.6 potassium nitrate, 0.05~0.06 ammonium dihydrogen phosphate, 0.1
~0.3 magnesium sulfate, 0.01~0.02 ferrous sulfate, 0.01~0.03 disodium ethylene diamine tetraacetate, 0.002~0.005 boric acid,
0.002~0.003 manganese sulfate, 0.0002~0.0005 zinc sulfate, 0.00006~0.0001 copper sulphate, 0.00002~
0.00006 ammonium molybdate and 1000 water.
In a preferred embodiment of the invention, above-mentioned cultural method includes the following steps：1) the first water planting is used to seek
Nutrient solution carries out water planting 7~14 days to romaine lettuce seedling, obtains romaine lettuce seedling；In first mill water culture nutrient solution Se content be 0.1~
0.2mg/L；In parts by weight, the first water planting nutrient solution further includes following component：0.2~0.3 calcium nitrate, 0.4~0.6 nitric acid
Potassium, 0.05~0.06 ammonium dihydrogen phosphate, 0.1~0.3 magnesium sulfate, 0.01~0.02 ferrous sulfate, 0.01~0.03 ethylenediamine tetraacetic
Acetic acid disodium, 0.002~0.005 boric acid, 0.002~0.003 manganese sulfate, 0.0002~0.0005 zinc sulfate, 0.00006~
0.0001 copper sulphate, 0.00002~0.00006 ammonium molybdate and 1000 water；
2) the second mill water culture nutrient solution is used to carry out water planting 7~21 days to the romaine lettuce seedling that step 1) obtains；Second water
Se content is 0.3~0.5mg/L in cultivation nutrient fluid；In parts by weight, second mill water culture nutrient solution further includes following component：
0.2~0.3 calcium nitrate, 0.4~0.6 potassium nitrate, 0.05~0.06 ammonium dihydrogen phosphate, 0.1~0.3 magnesium sulfate, 0.01~0.02
Ferrous sulfate, 0.01~0.03 disodium ethylene diamine tetraacetate, 0.002~0.005 boric acid, 0.002~0.003 manganese sulfate,
0.0002~0.0005 zinc sulfate, 0.00006~0.0001 copper sulphate, 0.00002~0.00006 ammonium molybdate and 1000 water；
3) the romaine lettuce seedling obtained to step 2) using first mill water culture nutrient solution carries out water planting, when romaine lettuce has 15~
20 true leaves can harvest.
Wherein, step 1) is typically usually to start first week after water planting to second week in the seedling separation growth period of romaine lettuce.
Step 2) is typically usually to start the third week after water planting to 4th week in the field planting growth period of romaine lettuce.Step 3) is typically
In the field planting growth period of romaine lettuce, usually start the third week after water planting to 4th week.When romaine lettuce seedling grows to 15~
It can be harvested when 20 true leaves.
In a preferred embodiment of the invention, above-mentioned cultural method further includes seeding and seedling raising step, and the sowing is educated
Seedling step specifically comprises the following steps：
Seedling medium containing romaine lettuce seed is placed in seedlings nursing plate, is cultivated 7~10 days at 15~25 DEG C of temperature, makes life
Dish seedling grows up to two leaves wholeheartedly, then the romaine lettuce seedling is inserted into the field planting hole on seedling-distributing plate, and the seedling-distributing plate is placed in
Water planting is carried out in breeding apparatus containing the mill water culture nutrient solution.
Wherein, the seedling medium is preferably made of multiple containing foraminate matrix fritter.The matrix is sponge, rock
Cotton, coconut palm chaff or diameter are less than the solid particle matrix of 2.5mm.Solid particle matrix can be vermiculite.Nursery used in seeding and seedling raising
Matrix should have fine air permeability, water-retaining property and buffer capacity.
Wherein, per unit area preferably has 300~330 field planting holes, further preferred 320 field planting on the seedling-distributing plate
Hole.Ensure there is seed in each field planting hole.
In seeding and seedling raising step, seed can be poured into the sucker of seeder, uniformly be rocked, seed is made to be adsorbed on suction
In the hole of disk, it is ensured that each hole has seed, and keeps the quality of every seed, and seedling medium is tipped upside down on seeder
On sucker (porose one downwards), bottom cover is covered, seeder is turned to and raised bottom cover, checks each seedling medium
There is seed in hole, seedling medium is moved in seedlings nursing plate, watering to seedling medium is sufficiently humidified so as to, in the case where temperature is 15~25 DEG C
It cultivates 7~10 days, romaine lettuce seedling grows up to two leaves wholeheartedly.
Wherein, romaine lettuce kind preferred cream romaine lettuce kind in the present invention, seed quality should meet the requirement of GB16715 standards,
Seed coat agent used in capsuled seed should meet the requirement of NY/T391 standards.
Wherein, it sows in the seeding and seedling raising step and is generally carried out in the morning.
Wherein, it should be noted that the control of temperature, illumination cannot be too strong in the seeding and seedling raising step during seeding and seedling raising, it is necessary
When can be used sunshade net adjust illumination.
In a preferred embodiment of the invention, when carrying out water planting to romaine lettuce seedling, preferably by the romaine lettuce seedling
The 70%~90% of root immerses in the mill water culture nutrient solution.
In a preferred embodiment of the invention, in step 2), the romaine lettuce seedling that step 1) obtains is displaced to unit
Area density is that the field planting plate is placed in second mill water culture nutrient solution and carries out water planting on the field planting plate in 25~30 holes.Often
Block, which is colonized on plate, contains 10~15 romaine lettuce seedlings.
In above-mentioned steps 1), 2) and 3) in, further include daily management.Daily management includes：Temperature and humidity is controlled, is adjusted
Whole illumination is periodically divulged information, the content of total nitrogen, total phosphorus and potassium in periodic detection water, and nutrient is replenished in time, and observation vegetables are long
Gesture periodically exchanges the weaker romaine lettuce of growing way to the relatively high region of rich nutritive material content, according to romaine lettuce growing way situation and
Water quality detection is as a result, ensure romaine lettuce growing way.
Wherein, the adjusting of illumination mainly uses inside and outside shading net in the daily management, and divulges information generally using top day
Window, exhaust fan and interior cycle.
When romaine lettuce seedling is continued water planting to romaine lettuce in step 3) there are 20 or so true leaves to harvest.
Romaine lettuce after harvesting lists after should packing in time, to ensure its fresh quality.If cannot list in time, 0 can be put into
It is stored in~5 DEG C of environment, storage life is generally 5~7 days.
Compared with prior art, the beneficial effects of the invention are as follows：
1, the present invention carries out water planting selenium-rich using specific mill water culture nutrient solution to romaine lettuce, with traditional soil selenium-rich method phase
Than selenium rich ability is stronger, while also reducing the pollution of pollution, heavy metal-polluted soil to vegetables to soil.
2, the present invention carries out sublevel for water planting romaine lettuce different stages of growth using the mill water culture nutrient solution containing selenium of various concentration
Section selenium-rich water planting fast implements the conversion that external source adds the organism organic selenium that selenium is high to physiological activity, is easily absorbed by the body, conversion
Rate reaches 80% or more, improves the nutritional qualities such as soluble protein, vitamin C, the chlorophyll in water planting romaine lettuce, grasps simultaneously
Make that method is easy, easy to operate and quick harvest.The formula nutritional of the present invention is balanced, can meet romaine lettuce nutritional need, and simultaneously
The amount of application for reducing nutrient improves romaine lettuce yield, saves fertilizer.The reasonable employment of the fertilizer such as magnesium, boron can enhance anti-
Sick ability increases yield, improves quality.
3, the selenium-rich water planting romaine lettuce that the present invention cultivates, realizes the effective way of human body selenium-supply, to push selenium-rich water planting
The sound development of vegetables industry makes the new measure for pushing whole people's supply Se scientific engineering, horizontal, complete to improving health of the masses
Kind national health policy promotes fitness-for-all significant.
Description of the drawings
Fig. 1 is that external source inorganic selenium is added in the embodiment of the present invention 1 --- the selenium-rich water planting romaine lettuce Se form of sodium selenite point
The liquid chromatogram atomic fluorescence mass spectrogram of analysis, wherein 1 selenomethionine, 2 selenocystines.
Fig. 2 is that external source Organic Selenium --- the selenium-rich water planting romaine lettuce selenium shape of DL- selenomethionines is added in the embodiment of the present invention 2
The liquid chromatogram atomic fluorescence mass spectrogram of state analysis, wherein 1 selenomethionine, 2 selenocystines.
Specific implementation mode
With reference to embodiment, the embodiment of the present invention is furthur described in detail.Following embodiment is used for
Illustrate the present invention, but is not limited to the scope of the present invention.
Embodiment 1
A kind of cultural method of selenium-rich romaine lettuce is present embodiments provided, is specifically comprised the following steps：
(1) seeding and seedling raising
Seed is poured into the sucker of seeder, is uniformly rocked, seed is made to be adsorbed in the hole of sucker, it is ensured that Mei Gekong
There is seed in hole, and keeps the quality of every seed；Seedling medium is tipped upside down on the sucker of seeder (porose one towards
Under), bottom cover is covered, seeder is turned to and raised bottom cover, checks in the hole of each breeding matrix there is seed；By nursery
Matrix moves in seedlings nursing plate, and watering to seedling medium is sufficiently humidified so as to, and 7~10 days romaine lettuce seedling are cultivated at being 15~25 DEG C in temperature
It grows to two leaves wholeheartedly；The cream romaine lettuce kind of this prosperous (China) seed Co., Ltd of romaine lettuce variety selection Rake, seed quality are answered
Meet the requirement of GB16715 standards, seed coat agent should meet the requirement of NY/T391 standards used in capsuled seed；Sowing generally the morning into
Row；Seedling medium used has fine air permeability, water-retaining property and buffer capacity, is composed of (on every piece alienable fritter
Have an aperture), as sponge, rock wool, coconut palm chaff or diameter are less than the solid particle matrix (such as vermiculite) of 2.5mm；It should be noted during nursery
The control for temperature of anticipating, illumination cannot be too strong, sunshade net can be used to adjust illumination when necessary.
(2) it is divided into seedlings growth period
When seedling separation, seedling medium is broken into two with one's hands, is then inserted into seedling in the seedling-distributing plate that per unit area density is 320 holes, point
Hand is light when seedling, not damage the seedling, seedling-distributing plate should stick with seedling, and seedling-distributing plate should be put into the first mill water culture nutrient solution after sticking with
In culture pond.
Mill water culture nutrient solution containing selenium is prepared by the raw material of following parts by weight：1000 water, 0.236 calcium nitrate, 0.404 nitric acid
Potassium, 0.057 ammonium dihydrogen phosphate, 0.123 magnesium sulfate, 0.0139 ferrous sulfate, 0.0186 disodium ethylene diamine tetraacetate, 0.00286
Boric acid, 0.00213 manganese sulfate, 0.00022 zinc sulfate, 0.00006 copper sulphate, 0.00002 ammonium molybdate, 0.0002 sodium selenite.
Romaine lettuce seedling separation growth period be beginning first week of water planting to second week, uses Se content for the first water of 0.2mg/L
Cultivation nutrient fluid carries out water planting.
(3) it is colonized growth period
When field planting, seedling is taken out from seedling-distributing plate, is displaced on the field planting plate that per unit area density is 25 holes, when field planting
Hand is light, not damage the seedling, and field planting plate should stick with seedling, and field planting plate should be put into the left sides the 10cm containing sodium selenite after sticking with
In the culture pond of right the second deep mill water culture nutrient solution, 15 romaine lettuce are planted on every block of field planting plate.
Mill water culture nutrient solution containing selenium is prepared by the raw material of following parts by weight：1000 water, 0.236 calcium nitrate, 0.404 nitric acid
Potassium, 0.057 ammonium dihydrogen phosphate, 0.123 magnesium sulfate, 0.0139 ferrous sulfate, 0.0186 disodium ethylene diamine tetraacetate, 0.00286
Boric acid, 0.00213 manganese sulfate, 0.00022 zinc sulfate, 0.00006 copper sulphate, 0.00002 ammonium molybdate, 0.0005 sodium selenite.
Growth period is colonized from third Zhou Zhi five weeks, uses Se content for the second mill water culture nutrient solution of 0.5mg/L progress water
Training.
(4) harvest time in later stage
It is changed to the first mill water culture nutrient solution after field planting growth period, when there are romaine lettuce 20 or so true leaves can harvest.
First mill water culture nutrient solution is prepared by the raw material of following parts by weight：1000 water, 0.236 calcium nitrate, 0.404 nitric acid
Potassium, 0.057 ammonium dihydrogen phosphate, 0.123 magnesium sulfate, 0.0139 ferrous sulfate, 0.0186 disodium ethylene diamine tetraacetate, 0.00286
Boric acid, 0.00213 manganese sulfate, 0.00022 zinc sulfate, 0.00006 copper sulphate, 0.00002 ammonium molybdate, 0.0002 sodium selenite.
Harvest time in later stage is start water planting the 6th week to the 7th week, and Se content is used to be sought for the first water planting of 0.2mg/L
Nutrient solution carries out water planting.
Romaine lettuce after harvesting lists after should packing in time, to ensure its fresh quality, if cannot list in time, can be put into
It is stored in 0-5 DEG C of environment, storage life is generally 5-7 days.
Embodiment 2
A kind of cultural method of selenium-rich romaine lettuce is present embodiments provided, is specifically comprised the following steps：
(1) seeding and seedling raising
Seed is poured into the sucker of seeder, is uniformly rocked, seed is made to be adsorbed in the hole of sucker, it is ensured that Mei Gekong
There is a seed in hole, and keeps the quality of every seed, seedling medium is tipped upside down on the sucker of seeder (porose one towards
Under), bottom cover is covered, seeder is turned to and raised bottom cover, checks in the hole of each breeding matrix there is seed, by nursery
Matrix moves in seedlings nursing plate, and watering to seedling medium is sufficiently humidified so as to, and 7~10 days romaine lettuce seedling are cultivated at being 15~25 DEG C in temperature
It grows to two leaves wholeheartedly；The cream romaine lettuce kind of this prosperous (China) seed Co., Ltd of romaine lettuce variety selection Rake, seed quality are answered
Meet the requirement of GB16715 standards, seed coat agent should meet the requirement of NY/T391 standards used in capsuled seed；Sowing generally the morning into
Row；Seedling medium used has fine air permeability, water-retaining property and buffer capacity, is composed of (on every piece alienable fritter
Have an aperture), as sponge, rock wool, coconut palm chaff or diameter are less than the solid particle matrix (such as vermiculite) of 2.5mm；It should be noted during nursery
The control for temperature of anticipating, illumination cannot be too strong, sunshade net can be used to adjust illumination when necessary.
(2) it is divided into seedlings growth period
When seedling separation, seedling medium is broken into two with one's hands, is then inserted into seedling in the seedling-distributing plate that per unit area density is 320 holes, point
Hand is light when seedling, not damage the seedling, seedling-distributing plate should stick with seedling, and seedling-distributing plate should be put into the first mill water culture nutrient solution after sticking with
In culture pond.
Mill water culture nutrient solution containing selenium is prepared by the raw material of following parts by weight：1000 water, 0.236 calcium nitrate, 0.404 nitric acid
Potassium, 0.057 ammonium dihydrogen phosphate, 0.123 magnesium sulfate, 0.0139 ferrous sulfate, 0.0186 disodium ethylene diamine tetraacetate, 0.00286
Boric acid, 0.00213 manganese sulfate, 0.00022 zinc sulfate, 0.00006 copper sulphate, 0.00002 ammonium molybdate, 0.0002DL- seleno eggs
Propylhomoserin.
Romaine lettuce seedling separation growth period be beginning first week of water planting to second week, uses Se content for the water containing selenium of 0.2mg/L
Cultivation nutrient fluid carries out water planting.
(3) it is colonized growth period
When field planting, seedling is taken out from seedling-distributing plate, is displaced on the field planting plate that per unit area density is 25 holes, when field planting
Hand is light, not damage the seedling, and field planting plate should stick with seedling, and field planting plate should be put into containing DL- selenomethionines after sticking with
In the culture pond of second mill water culture nutrient solution of 10cm or so depths, 15 romaine lettuce are planted on every block of field planting plate.
Mill water culture nutrient solution containing selenium is prepared by the raw material of following parts by weight：1000 water, 0.236 calcium nitrate, 0.404 nitric acid
Potassium, 0.057 ammonium dihydrogen phosphate, 0.123 magnesium sulfate, 0.0139 ferrous sulfate, 0.0186 disodium ethylene diamine tetraacetate, 0.00286
Boric acid, 0.00213 manganese sulfate, 0.00022 zinc sulfate, 0.00006 copper sulphate, 0.00002 ammonium molybdate, 0.0005DL- seleno eggs
Propylhomoserin.
Growth period is colonized from third Zhou Zhi five weeks, uses Se content for the mill water culture nutrient solution containing selenium of 0.5mg/L progress water
Training.
(4) harvest time in later stage
Mill water culture nutrient solution containing selenium is replaced after being colonized growth period；When there are romaine lettuce 20 or so true leaves can harvest.
Mill water culture nutrient solution containing selenium is prepared by the raw material of following parts by weight：1000 water, 0.236 calcium nitrate, 0.404 nitric acid
Potassium, 0.057 ammonium dihydrogen phosphate, 0.123 magnesium sulfate, 0.0139 ferrous sulfate, 0.0186 disodium ethylene diamine tetraacetate, 0.00286
Boric acid, 0.00213 manganese sulfate, 0.00022 zinc sulfate, 0.00006 copper sulphate, 0.00002 ammonium molybdate, 0.0002DL- seleno eggs
Propylhomoserin.
Harvest time in later stage is start water planting the 6th week to the 7th week, is carried out using the mill water culture nutrient solution containing selenium of 0.2mg/L
Water planting.
Romaine lettuce after harvesting lists after should packing in time, to ensure its fresh quality, if cannot list in time, can be put into
It is stored in 0-5 DEG C of environment, storage life is generally 5-7 days.
Experimental example
Data Detection and analysis：
(1) biological yield and nutriment to the selenium-rich water planting romaine lettuce for not applying selenium water planting romaine lettuce group and Examples 1 and 2 respectively
Matter is measured, and the results are shown in Table 1.Compared with the control group, addition external source selenium increases the biological yield of water planting romaine lettuce, carries
The content of vitamin C, soluble protein, chlorophyll, improves the quality of romaine lettuce in high water planting romaine lettuce.
The measurement result of 1 selenium-rich water planting romaine lettuce biological yield of table and nutritional quality
(2) in Examples 1 to 2 the testing results such as total selenium of water planting romaine lettuce, Organic Selenium, organic Se form respectively such as 2 and of table
Shown in Fig. 1, Fig. 2.As shown in Table 2, the addition of external source inorganic selenium and Organic Selenium greatly increases total selenium and Organic Selenium in water planting romaine lettuce
Content, total Se content are 1182.54 times and 1090.48 times of control group respectively, and organic selenium content is control group respectively
1079.49 times and 958.12 times, overall absorption high conversion rate is up to 80% or more.As seen from Figure 1, Figure 2, embodiment 1 and embodiment 2
Selenomethionine ratio is up to 88% or more in middle water planting romaine lettuce, is secondly selenocystine, it was reported that selenomethionine can
Caused by tumor suppressor p 53 is activated, illustrates that selenium-rich romaine lettuce has preferable healthcare function.
The testing result (mg/kg, DW) of total selenium, Organic Selenium and sorption enhanced rate in 2 water planting romaine lettuce of table
| Total Se content | Inorganic Se content | Organic selenium content | Sorption enhanced rate/% | |
| Control group | 0.0126 | 0.0009 | 0.0117 | 92.86 |
| Embodiment 1 | 14.90 | 2.27 | 12.63 | 84.77 |
| Embodiment 2 | 13.74 | 2.53 | 11.21 | 81.59 |
The present invention adds various concentration external source selenium in water planting romaine lettuce different stages of growth, is improving biological yield and improvement
While quality, also converting external source addition selenium preparation to the biology that physiological activity is high, is easily absorbed by the body by organism has
Machine selenium, and operating method is simple, the period is short.
Finally, method of the invention is only preferable embodiment, is not intended to limit the scope of the present invention.It is all
Within the spirit and principles in the present invention, any modification, equivalent replacement, improvement and so on should be included in the protection of the present invention
Within the scope of.
Claims (10)
1. a kind of cultural method of selenium-rich romaine lettuce, which is characterized in that include the following steps：
1) it uses the first mill water culture nutrient solution to carry out water planting 7~14 days to romaine lettuce seedling, obtains romaine lettuce seedling；The first water planting battalion
Se content is 0.1~0.2mg/L in nutrient solution；
2) the second mill water culture nutrient solution is used to carry out water planting 7~21 days to the romaine lettuce seedling that step 1) obtains；The second water planting battalion
Se content is 0.3~0.5mg/L in nutrient solution；
3) water planting is carried out to the romaine lettuce seedling that step 2) obtains using first mill water culture nutrient solution.
2. cultural method according to claim 1, which is characterized in that in parts by weight, the first water planting nutrient solution and
Two water planting nutrient solutions further include following component：0.2~1.0 calcium nitrate, 0.1~0.6 potassium nitrate, 0.05~0.3 ammonium dihydrogen phosphate,
0.1~0.7 magnesium sulfate, 0.01~0.03EDTA chelates, 0.002~0.006 boric acid, 0.002~0.004 manganese sulfate,
0.0002~0.0005 zinc sulfate, 0.00006~0.0001 copper sulphate, 0.00002~0.0001 ammonium molybdate and 1000 water.
3. cultural method according to claim 2, which is characterized in that in parts by weight, the first water planting nutrient solution and
Two water planting nutrient solutions further include following component：0.01~0.05 ferrous sulfate.
4. cultural method according to any one of claim 1 to 3, which is characterized in that the first water planting nutrient solution and/or
Second water planting nutrient solution further includes following component：0.2~0.3 calcium nitrate, 0.4~0.6 potassium nitrate, 0.05~0.06 biphosphate
Ammonium, 0.1~0.3 magnesium sulfate, 0.01~0.02 ferrous sulfate, 0.01~0.03 disodium ethylene diamine tetraacetate, 0.002~0.005
Boric acid, 0.002~0.003 manganese sulfate, 0.0002~0.0005 zinc sulfate, 0.00006~0.0001 copper sulphate, 0.00002~
0.00006 ammonium molybdate and 1000 water；
And/or the first water planting nutrient solution and/or the second water planting nutrient solution further include following component：0.9~1.0 calcium nitrate,
0.2~0.5 potassium nitrate, 0.1~0.3 potassium dihydrogen phosphate, 0.5~0.7 magnesium sulfate, 0.01~0.03EDTA-Fe, 0.003~
0.006 boric acid, 0.002~0.004 manganese sulfate, 0.0002~0.0005 zinc sulfate, 0.00008~0.0001 copper sulphate,
0.00002~0.0001 ammonium molybdate and 1000 water.
5. cultural method according to any one of claim 1 to 4, which is characterized in that include the following steps：
1) it uses the first mill water culture nutrient solution to carry out water planting 7~14 days to romaine lettuce seedling, obtains romaine lettuce seedling；The first water planting battalion
Se content is 0.1~0.2mg/L in nutrient solution；In parts by weight, the first water planting nutrient solution further includes following component：0.2~0.3
Calcium nitrate, 0.4~0.6 potassium nitrate, 0.05~0.06 ammonium dihydrogen phosphate, 0.1~0.3 magnesium sulfate, 0.01~0.02 ferrous sulfate,
0.01~0.03 disodium ethylene diamine tetraacetate, 0.002~0.005 boric acid, 0.002~0.003 manganese sulfate, 0.0002~0.0005
Zinc sulfate, 0.00006~0.0001 copper sulphate, 0.00002~0.00006 ammonium molybdate and 1000 water；
2) the second mill water culture nutrient solution is used to carry out water planting 7~21 days to the romaine lettuce seedling that step 1) obtains；The second water planting battalion
Se content is 0.3~0.5mg/L in nutrient solution；In parts by weight, second mill water culture nutrient solution further includes following component：0.2~
0.3 calcium nitrate, 0.4~0.6 potassium nitrate, 0.05~0.06 ammonium dihydrogen phosphate, 0.1~0.3 magnesium sulfate, 0.01~0.02 sulfuric acid are sub-
Iron, 0.01~0.03 disodium ethylene diamine tetraacetate, 0.002~0.005 boric acid, 0.002~0.003 manganese sulfate, 0.0002~
0.0005 zinc sulfate, 0.00006~0.0001 copper sulphate, 0.00002~0.00006 ammonium molybdate and 1000 water；
3) water planting is carried out to the romaine lettuce seedling that step 2) obtains using first mill water culture nutrient solution, when romaine lettuce has 15~20
True leaf can harvest.
6. cultural method according to any one of claim 1 to 5, which is characterized in that the cultural method further includes broadcasting
Kind nursery step, the seeding and seedling raising step specifically comprise the following steps：
Seedling medium containing romaine lettuce seed is placed in seedlings nursing plate, is cultivated 7~10 days at 15~25 DEG C of temperature, keeps romaine lettuce young
Seedling grow up to two leaves wholeheartedly, then by the romaine lettuce seedling be inserted into seedling-distributing plate on field planting hole in, by the seedling-distributing plate be placed in containing
Water planting is carried out in the breeding apparatus of the mill water culture nutrient solution.
7. cultural method according to claim 6, which is characterized in that the seedling medium contains foraminate matrix by multiple
Fritter forms；The matrix is the solid particle matrix that sponge, rock wool, coconut palm chaff or diameter are less than 2.5mm.
8. the cultural method described according to claim 6 or 7, which is characterized in that on the seedling-distributing plate per unit area have 300~
330 field planting holes, preferably 320 field planting holes.
9. cultural method according to any one of claim 1 to 8, which is characterized in that when carrying out water planting to romaine lettuce seedling,
The 70%~90% of the root of the romaine lettuce seedling is immersed in the mill water culture nutrient solution.
10. cultural method according to any one of claim 1 to 9, which is characterized in that in step 2), step 1) is obtained
To romaine lettuce seedling be displaced to per unit area density be 25~30 holes field planting plate on, the field planting plate is placed in second water
Water planting is carried out in cultivation nutrient fluid；Contain 10~15 romaine lettuce seedlings on every block of field planting plate.
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