CN108094168A - A kind of method that selenium-rich romaine lettuce is produced under the conditions of plant factor - Google Patents
A kind of method that selenium-rich romaine lettuce is produced under the conditions of plant factor Download PDFInfo
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- A—HUMAN NECESSITIES
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- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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- A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
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Abstract
The invention discloses a kind of methods that selenium-rich romaine lettuce is produced under the conditions of plant factor, include the following steps：S1：After making romaine lettuce germination, nursery, culture to the seedling for exposing 3~4 true leaves are carried out；S2：Seedling replanting is simultaneously colonized；S3：After transplant planting, the seedling of 3~4 true leaves is positioned over to selenium concentration is 0.05~0.5mg/L, intensity of illumination is 125~575 μm of olm‑2·s‑1, cultivate 25~30 days under red and blue LED lamp after obtain selenium-rich romaine lettuce.The present invention is using red and blue LED Control Console, when external source selenium concentration is 0.20mg/L, biomass, comprehensive nutrient quality, organic selenium content and the conversion ratio highest of romaine lettuce；Intensity of illumination is 300 μm of olm‑2·s‑1When, biomass, comprehensive nutrient quality, the content of Organic Selenium and the conversion ratio highest of selenium-rich romaine lettuce；It is 6 in red blue light matter proportioning:Under the conditions of 1, biomass, nutritional quality, Se content and the accumulation of romaine lettuce are maximum, and external source selenium concentration is 0.20mg/L in nutrient solution, intensity of illumination is 300 μm of olm‑2·s‑1, red blue light matter proportioning be 6:1 is the optimum combination of romaine lettuce selenium-rich production under the conditions of plant factor.
Description
Technical field
The present invention relates to a kind of modern agriculture planting technologies, and in particular to the cultivation of selenium-rich romaine lettuce under the conditions of a kind of plant factor
Culture method.
Background technology
Selenium is that human body and a kind of necessary trace element of animal cannot synthesize in vivo, it is necessary to be absorbed from food.At present
China common people are generally relatively low to the intake of selenium, about 26mg/d, less than the lower limit for the physiology necessary amount recommended, belong to
Low selenium scope.For human body is made to improve to the scope recommended the intake of selenium, expert advice is using rich selenium vegetables as the battalion of selenium
Replenishers are supported, it is horizontal to the intake of selemium nutrition so as to improve human body.
Just one of factor important in plant growth and development process is the energy source of photosynthesis of plant, it passes through
The growth and development of plant, morphogenesis and physiology generation are influenced in terms of light quality, light intensity (photon flux density) and light application time three
It thanks, key effect is played during the yield and quality of crop is formed.The adjusting of warm Interior Illumination Environment is made to improving greenhouse
The yield and quality of object play a crucial role.
Romaine lettuce (Lactucasativa) is also known as Leaf lettuce, belongs to composite family Lactuca, is planted for annual or biennial herb
Object, as a kind of worldwide vegetable.Romaine lettuce nutritive value is high, have adjust nervous system, promote blood circulation, Li Shui disappears
Swollen and fat-eliminating slimming and other effects.In recent years, romaine lettuce becomes one of main leaf vegetables kind in facility cultivation.
Suitable external source selenium processing and luminous environment can improve the yield of vegetables, improve its nutritional quality.But so far not yet
It sees to cooperate with luminous environment regulation and control on the processing of water planting selenium-rich and improves romaine lettuce yield, improves nutritional quality and improve selenium-rich characteristic
Report.
The content of the invention
The present invention, using red and blue LED lamp and the mode of water planting, carries out romaine lettuce seedling different under the conditions of plant factor
Selenium concentration, intensity of illumination, the processing of light quality proportioning, filter out the selenium concentration and the ring of light for being conducive to that selenium-rich romaine lettuce is produced in plant factor
Border is combined, and so as to improve the yield and quality of selenium-rich romaine lettuce, produces the selenium-rich of high yield and high quality under the conditions of plant factor for people
Romaine lettuce provides a kind of production technology.
The technical solution adopted in the present invention：A kind of method that selenium-rich romaine lettuce is produced under the conditions of plant factor, including as follows
Step：
S1：After making romaine lettuce germination, nursery, culture to the seedling for exposing 3~4 true leaves are carried out；
S2：Seedling replanting is simultaneously colonized；
S3：After transplant planting, the seedling of 3~4 true leaves is positioned over selenium concentration as 0.05~0.5mg/L, intensity of illumination
For 125~575 μm of olm-2·s-1, cultivate 25~30 days under red and blue LED lamp after obtain selenium-rich romaine lettuce.
In step S3, red blue light matter proportioning is 2:1~6:1.
In step S3, red light wavelength is 655~660nm, peak value 657.5nm；Blue light wavelength be 455~460nm, peak value
457.5nm。
In step S1, training method is specially in suspension culture culture.
In step S1, for the nutrient solution a great number of elements formula using 1/2 Glan formula suddenly, trace element is general formulation,
Include B：0.5mg·L-1, Mn：0.5mg·L-1, Zn：0.05mg·L-1, Cu：0.02mg·L-1, Mo：0.01mg·L-1。
In step S1, nutrient solution is changed without after Seeding planting, is supplemented the nutrient solution with deionized water within every 15~16 days
To original volume.
In step S3, selenium concentration 0.20mg/L, intensity of illumination are 300 μm of olm-2·s-1, red blue light matter proportioning is 6:
1。
In step S3, when periodicity of illumination is 12~16 small, cultivation temperature is 25 DEG C, and medial humidity control is 85%.
The illumination time started is 5~7 points, and the end time is 17~23 points.
Compared with prior art, the beneficial effects of the invention are as follows：(1) selenium-rich romaine lettuce is produced under the conditions of a kind of plant factor
Cultural method under the conditions of plant factor, using red and blue LED Control Console and the mode of water planting, carries out romaine lettuce seedling optimal
Selenium concentration, intensity of illumination, light quality proportioning combined treatment, can produce the good selenium-rich romaine lettuce of yield height, good quality, ornamental value, real
Water planting selenium-rich romaine lettuce is efficiently produced in existing plant factor.(2) in plant factor selenium-rich romaine lettuce is produced using red and blue LED Control Console
It is an efficient and easy-to-use new technology, advantage protrudes.
Description of the drawings
Fig. 1 is influence of the various concentration selenium to lettuce quality.
Wherein：The selenium concentration CK of test process：0mg/L、T1：0.05mg/L、T2：0.20mg/L、T3：0.35mg/L、T4：
0.5mg/L.Significantly (α=0.05), Fig. 2-Fig. 9 is similarly for different lowercase letter indication differences on figure column.
Fig. 2 is influence of the various concentration selenium to romaine lettuce oxidation resistance.
Fig. 3 is for various concentration selenium to romaine lettuce overground part beneficial to the influence of Mineral Elements Content.
Fig. 4 is influence of the different selenium concentrations to the full selenium accumulation of romaine lettuce overground part.
Fig. 5 is influence of the different illumination intensity to lettuce quality.
Fig. 6 is influence of the different illumination intensity to romaine lettuce oxidation resistance.
Fig. 7 is for different illumination intensity to romaine lettuce overground part beneficial to the influence of Mineral Elements Content.
Fig. 8 is for different illumination intensity to romaine lettuce overground part beneficial to the influence of Mineral Element Accumulation amount.
Fig. 9 is influence of the different light intensity to the full selenium accumulation of romaine lettuce overground part.
Figure 10 different light mediums are with the influence for comparing lettuce quality.
Wherein：T1：R:B=2:1、T2：R:B=4:1、T3：R:B=6:1、T4：80%4R/1B+20%G, on figure column not
With lowercase letter indication difference significantly (α=0.05), Figure 11 is similarly.
Figure 11 different light mediums are with the influence for comparing romaine lettuce selenium total amount and full selenium accumulation.
Specific embodiment
The technical solution further illustrated the present invention with reference to specific embodiment.
Embodiment
1. material
Test in July, 9~2017 in 2016 College of Horticulture of Agricultural University Of South China controllable environment room and vegetable growing with
It is carried out in Physiological Experiment room.It is " the Italian romaine lettuce of resistance to bolting " for examination romaine lettuce kind, test process uses red and blue LED Control Console,
Wherein red light wavelength is 657.5nm, blue light wavelength 457.5nm.Experiment is blue plastic tub with cultivation case, and basin inner volume is
60cm×40cm×12cm.For nutrient solution a great number of elements formula using 1/2 Glan formula (pH value ≈ 6.0) suddenly, trace element is logical
With formula, that is, include B：0.5mg·L-1, Mn：0.5mg·L-1, Zn：0.05mg·L-1, Cu：0.02mg·L-1, Mo：
0.01mg·L-1.Its step is：Step S1：After making romaine lettuce germination, nursery, culture to the children for exposing 3~4 true leaves are carried out
Seedling；Step S2 seedling replantings are simultaneously colonized；The seedling of 3~4 true leaves is positioned over different selenium concentrations, intensity of illumination, red by step S3
The culture of blue light matter proportioning obtains selenium-rich romaine lettuce after 25~30 days.Entire breeding time is changed without nutrient solution after romaine lettuce field planting, every 15d
Original volume is supplemented to deionized water, training method is specially in suspension culture culture.All cultivation techniques of the present invention are equal
In semi-enclosed indoor progress, from the influence of temperature, illumination condition, the humidity under in any natural conditions etc..
2. test method and result
Influence of the 2.1 various concentration selenium to lettuce quality, Nutrient Absorption and Se content
It tests and was carried out in September in 2016 to November 12 in College of Horticulture of Agricultural University Of South China controllable environment room within 25th.Examination
It tests and sets 4 processing altogether, external source selenium (Na in nutrient solution2SeO3) concentration is respectively：
T1=0.05mg/L, T2=0.20mg/L, T3=0.35mg/L, T4=0.5mg/L and 1 control treatment CK=
0mg/L.Red blue light matter proportioning is R：B=4：1, intensity of illumination is unified for 350 μm of olm-2·s-1, photoperiod 16h/d, put down
Equal temperature control is 25 DEG C, and medial humidity control is 85%.Romaine lettuce seed is positioned over vernalization in growth cabinet, vernalization condition
For：25 degrees Celsius.Seed shows money or valuables one carries unintentionally after 48h, and point is multicast to sponge block center.September is sowed on the 25th, October 12 three leaves wholeheartedly when
Selecting the uniform romaine lettuce seedling replanting of growing way extremely the 1/2 of loading 23L, Glan is formulated in hydroponic box suddenly, is positioned over LED light plate
Underface 50cm at carry out test process.November 12 harvested, measure Growth of Lettuce development, nutritional quality, Nutrient Absorption and
The indexs such as different shape selenium element content.
Influence of 2.2 different illumination intensities to lettuce quality, Nutrient Absorption and Se content
Test on January 2,15 days to 2017 November in 2016 College of Horticulture of Agricultural University Of South China controllable environment room into
Row.External source selenium (the Na in nutrient solution2SeO3) concentration is illumination under conditions of 0.20mg/L, 5 different illumination intensities is set to handle
Respectively T1=125 μm of olm-2·s-1, T2=200 μm of olm-2·s-1, T3=300 μm of olm-2·s-1, T4=425 μ
mol·m-2·s-1, T5=575 μm of olm-2·s-1.The LED illumination condition of above-mentioned processing is that red blue light matter proportioning is R：B=
4：1, light application time 16h/d；Environment mean temperature is 25 DEG C, medial humidity 85%.Romaine lettuce seed is positioned over artificial climate
Vernalization in case, vernalization condition are：25 degrees Celsius.Seed shows money or valuables one carries unintentionally after 48h, and point is multicast to sponge block center.November 15 was sowed,
December 2 three leaves wholeheartedly when select select growing way uniform romaine lettuce seedling replanting to be packed into the 1/2 of 23L suddenly Glan formula plant
It trains in case, is positioned over progress test process at the underface 50cm of LED light plate.On January 12nd, 2017 harvests, and measures Growth of Lettuce
The indexs such as development, nutritional quality, Nutrient Absorption and different shape selenium element content.
2.3 different light mediums are with the influence for comparing lettuce quality, Nutrient Absorption and Se content
Test on July 15,2 days to 2017 June in 2017 College of Horticulture of Agricultural University Of South China controllable environment room into
Row.External source selenium (the Na in nutrient solution2SeO3) concentration is 0.20mg/L, intensity of illumination is 300 μm of olm-2·s-1Under conditions of,
4 different light medium proportionings is set to handle, are respectively that red blue light matter proportioning is R:B=2:1、4:1、6:1, red bluish-green light quality, which matches, is
80%4R/1B+20%G；Light application time is 16h/d, and environment mean temperature is 25 DEG C, medial humidity 85%.Romaine lettuce seed is put
Vernalization in growth cabinet is placed in, vernalization condition is：25 degrees Celsius.Seed shows money or valuables one carries unintentionally after 48h, and point is multicast to sponge block center.June 2
Day sowed, June 20 three leaves wholeheartedly when select and select the uniform romaine lettuce seedling replanting of growing way to being packed into the 1/2 of 23L
In Huo Gelan formula cultivation casees, progress test process at the underface 50cm of LED light plate is positioned over.July 15 harvested, and measured life
The indexs such as dish growth and development, nutritional quality, Nutrient Absorption and different shape selenium element content.
Test data carries out data analysis using SPSSStatistics17.0, and variance is carried out using LSD multiple comparison graphs
Analysis, statistical result are showed in the form of " average value ± standard error ", mapped using GrapPadPrism6.And by data record
Enter table 1~8 and Fig. 1~11.
Influence of the 1 various concentration selenium of table to Growth of Lettuce
Note：Red blue light matter proportioning is R:B=4:1, external source selenium Na2SeO3Concentration is CK=0mg/L, T1=0.05mg/L, T2
=0.20mg/L, T3=0.35mg/L, T5=0.5mg/L.Same column difference lowercase letter indication difference reaches 5% level of signifiance,
Similarly hereinafter.
Influence of the 2 various concentration selenium of table to romaine lettuce Contents of Photosynthetic Pigments
Influence of the 3 external source selenium of table to different shape selenium element content in romaine lettuce
Influence of 4 different illumination intensity of table to Growth of Lettuce
Note：In the case where external source selenium concentration is 0.20mg/L, T1=125 μm of olm of different intensities of illumination is set-2·s-1、T2
=200 μm of olm-2·s-1, T3=300 μm of olm-2·s-1, T4=425 μm of olm-2·s-1, T5=575 μm of olm-2·
s-1。
Influence of 5 different illumination intensity of table to romaine lettuce Photosynthetic Pigment
Influence of 6 different illumination intensity of table to different shape selenium element content in romaine lettuce
7 different light medium of table is with the influence for comparing Growth of Lettuce
Note：External source selenium concentration is 0.20mg/L, and intensity of illumination is 300 μm of olm-2·s-1, light quality proportioning T1：R:B=2:
1、T2：R:B=4:1、T3：R:B=6:1、T4：80%4R/1B+20%G, similarly hereinafter.
8 different light medium of table is with the influence for comparing romaine lettuce photosynthetic pigments
3. result and analysis
Influence of the 3.1 various concentration selenium to lettuce quality, Nutrient Absorption and Se content
As shown in Table 1, from the point of view of comprehensive growth indexes, romaine lettuce yield shows elder generation with the increase of external source selenium concentration in nutrient solution
Increase the trend then reduced.Low concentration selenium plays a driving role to the growth of plant, and high concentration can then inhibit the life of plant
It is long.Influence of the various concentration selenium to romaine lettuce blade Contents of Photosynthetic Pigments is as shown in table 2.Compared with other processing, the light of T4 processing
It is maximum with pigment content.As shown in Figure 1, romaine lettuce soluble protein content is issued to maximum 7.48mg/g, T2, T3 in T2 processing
The lower romaine lettuce blade middle reaches content in bleeding sap from stem of processing is significantly higher than other three processing.Vc contents and soluble sugar content are with selenium
The rise of concentration and show first increase after downward trend, the two reach when T3 handle maximum.
Various concentration selenium is as shown in Figure 3 beneficial to the influence of Mineral Elements Content to romaine lettuce overground part.Low concentration in nutrient solution
Selenium the content of the full zinc of romaine lettuce overground part and full iron is influenced little, but the selenium of high concentration can improve overground part beneficial to mineral element
Content, as shown in table 3 and fig. 4, organic selenium content is with external source in dry sample for influence of the various concentration selenium to selenium element absorption in romaine lettuce
The increase of selenium concentration first increases to be reduced afterwards, and organic selenium content reaches 22.01 μ g/g of maximum in the lower dry sample of T2 processing, is significantly higher than
Other processing.Organic Selenium conversion ratio is consistent with the variation tendency of organic selenium content in dry sample in romaine lettuce overground part dry sample, in T2
Conversion ratio highest during processing.In conclusion T2 processing is put up the best performance in terms of Growth of Lettuce and improving quality is promoted, suitable concentration
Selenium can improve the biomass of romaine lettuce, comprehensive nutrient quality, improve organic selenium content and conversion ratio.
Influence of 3.2 different illumination intensities to lettuce quality, Nutrient Absorption and Se content
Influence of the different illumination intensity to Growth of Lettuce index is as shown in table 4.Every growth indexes of romaine lettuce are with light
Increase according to the increase of intensity, T3 processing shows more excellent in terms of growth indexes；As shown in table 5, each Contents of Photosynthetic Pigments is in
Reveal the trend continuously decreased with the increase of intensity of illumination, compared with T1 processing, the lower chlorophyll a of T2, T3, T4 and T5 processing
Content has been remarkably decreased 20.28%, 48.95%, 58.39%, 26.57% respectively.Different illumination intensity is to the shadow of lettuce quality
It rings as shown in figure 5, within the specific limits, the soluble sugar and Vc contents of romaine lettuce increase with the increase of light intensity, and light intensity has
Too strong to reduce free aminoacid content, T4 soluble protein contents are maximum, and difference is not notable between other each processing.Suitable light
The nutritional quality of romaine lettuce can be improved according to intensity.The increase of light intensity is also beneficial to improve the anti-oxidant of selenium-rich romaine lettuce in a certain range
Ability.
As shown in Figure 7 and Figure 8, different illumination intensity to romaine lettuce overground part beneficial to the totality of Mineral Elements Content and accumulation
The trend reduced is shown, reaches maximum when T3 is handled.Different illumination intensity is to the influence of selenium element absorption and ground in romaine lettuce
Dry sample full selenium accumulation in top is as shown in table 6 and Fig. 9.Full Se content, inorganic Se content, organic selenium content are equal in selenium-rich romaine lettuce dry sample
First increase with the increase of light intensity and reduce afterwards, the organic selenium content highest under T3 processing, and it is notable with other four processing differences.
3.3 different light mediums are with the influence for comparing lettuce quality and Se content
As shown in table 7, in terms of growth indexes, long, the maximum leaf leaf width of maximum leaf leaf, the overground part dry weight and fresh weight of plant seedlings of selenium-rich romaine lettuce
The trend of rising all is showed with the increase of feux rouges ratio, (red blue proportioning is R in T4 processing:B=4:1 time increase by 20%
Green light) be issued to maximum, it is but not notable with T3 processing differences.As shown in table 8, chlorophyll a, chlorophyll b, the Ye Lv of T4 processing
Plain total amount and carotenoid content are significantly better than other three processing, and illustrate increases green light under red blue proportioning to improve
Chlorophyll content.Different light medium is as shown in Figure 10 with the influence for comparing lettuce quality, the V of T3 processingCContent is significantly higher than other
Three processing, the soluble sugar and soluble protein content of T4 processing are significantly higher than other three processing, and what is respectively handled is free
Amino acid content difference is not notable.Different light medium proportioning under each processing selenium total amount it is as shown in figure 11, T3 processing selenium total amount and
Full selenium accumulation is maximum, and is significantly higher than other 3 processing.
4. conclusion
Under red blue light proportioning, nutrient solution adds selenium that can significantly improve the biomass of romaine lettuce, and external source selenium concentration is 0.2mg/L
When, romaine lettuce biomass is maximum, and nutritional quality is optimal；Add selenium that can promote absorption and the product of the full zinc of romaine lettuce overground part and full iron ion
It is tired, and in the case where external source selenium concentration is the processing of 0.2mg/L, full Se content and Organic Selenium conversion ratio are maximum in dry sample.Plant factor
Under the conditions of use 300 μm of olm-2·s-1Light intensity can promote selenium-rich Growth of Lettuce, improving quality；Romaine lettuce overground part iron and zinc
Constituent content accumulation reaches maximum, and full Se content, the conversion ratio highest of Organic Selenium.Red blue light matter proportioning is 6:Under conditions of 1,
Biomass, nutritional quality, Se content and the accumulation of romaine lettuce are maximum.In conclusion external source selenium 0.2mg/L, light intensity is 300 μ
mol·m-2·s-1, red blue light matter proportioning is 6:1 is the optimal combination of production selenium-rich romaine lettuce under the conditions of plant factor.
For those skilled in the art, technical solution that can be as described above and design are made other each
Kind is corresponding to be changed and deforms, and all these change and deform the protection model that should all belong to the claims in the present invention
Within enclosing.
Claims (9)
1. the method for selenium-rich romaine lettuce is produced under the conditions of a kind of plant factor, it is characterised in that：Include the following steps：
S1：After making romaine lettuce germination, nursery, culture to the seedling for exposing 3~4 true leaves are carried out；
S2：Seedling replanting is simultaneously colonized；
S3：After transplant planting, by the seedling of 3~4 true leaves be positioned over selenium concentration be 0.05~0.5mg/L, intensity of illumination 125
~575 μm of olm-2·s-1, cultivate 25~30 days under red and blue LED lamp after obtain selenium-rich romaine lettuce.
2. the method for selenium-rich romaine lettuce is produced under the conditions of a kind of plant factor according to claim 1, it is characterised in that：Step
In S3, red blue light matter proportioning is 2:1~6:1.
3. the method for selenium-rich romaine lettuce is produced under the conditions of a kind of plant factor according to claim 1, it is characterised in that：Step
In S3, red light wavelength is 655~660nm, peak value 657.5nm；Blue light wavelength is 455~460nm, peak value 457.5nm.
4. the method for selenium-rich romaine lettuce is produced under the conditions of a kind of plant factor according to claim 1, it is characterised in that：Step
In S1, training method is specially in suspension culture culture.
5. the method for selenium-rich romaine lettuce is produced under the conditions of a kind of plant factor according to claim 4, it is characterised in that：Step
In S1, the nutrient solution a great number of elements formula is general formulation using 1/2 Glan formula suddenly, trace element, that is, includes B：
0.5mg·L-1, Mn：0.5mg·L-1, Zn：0.05mg·L-1, Cu：0.02mg·L-1, Mo：0.01mg·L-1。
6. the method for selenium-rich romaine lettuce is produced under the conditions of a kind of plant factor according to claim 1, it is characterised in that：Step
In S1, nutrient solution is changed without after Seeding planting, the nutrient solution is supplemented to original volume with deionized water within every 15~16 days.
7. the method for selenium-rich romaine lettuce is produced under the conditions of a kind of plant factor according to claim 1, it is characterised in that：Step
In S3, selenium concentration 0.20mg/L, intensity of illumination are 300 μm of olm-2·s-1, red blue light matter proportioning is 6:1.
8. the method for selenium-rich romaine lettuce is produced under the conditions of a kind of plant factor according to claim 1, it is characterised in that：Step
In S3, when periodicity of illumination is 12~16 small, cultivation temperature is 25 DEG C, and medial humidity control is 85%.
9. the method for selenium-rich romaine lettuce is produced under the conditions of a kind of plant factor according to claim 1, it is characterised in that：Illumination
Time started is 5~7 points, and the end time is 17~23 points.
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| CN109122249A (en) * | 2018-06-25 | 2019-01-04 | 华南农业大学 | A kind of method that LED intermittent illumination cultivates cucumber seedling |
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| CN110881333A (en) * | 2019-12-24 | 2020-03-17 | 成都市三禾田生物技术有限公司 | Method for improving organic selenium content of broccoli sprouting vegetable |
| CN111406628A (en) * | 2020-04-30 | 2020-07-14 | 京东方后稷科技(北京)有限公司 | Artificial illumination planting method for butter lettuce |
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| CN114128435A (en) * | 2021-10-21 | 2022-03-04 | 湖南美食流智慧农业科技有限公司 | Selenium-rich lettuce and cultivation method thereof |
| CN114375781A (en) * | 2021-04-21 | 2022-04-22 | 北京农学院 | Selenium-rich lettuce hydroponic production method |
| CN114586561A (en) * | 2022-03-18 | 2022-06-07 | 华南农业大学 | Method for cultivating tomato seedlings by LED pulse illumination |
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| CN109122264A (en) * | 2018-08-23 | 2019-01-04 | 华南师范大学 | A kind of method that additional uv b radiation improves Greenhouse Water Culture romaine lettuce Flavonoid Content |
| CN109769677A (en) * | 2019-03-22 | 2019-05-21 | 福建省中科生物股份有限公司 | A kind of plant factor's Radix Isatidis ciltivating process |
| CN110881333A (en) * | 2019-12-24 | 2020-03-17 | 成都市三禾田生物技术有限公司 | Method for improving organic selenium content of broccoli sprouting vegetable |
| CN111406628A (en) * | 2020-04-30 | 2020-07-14 | 京东方后稷科技(北京)有限公司 | Artificial illumination planting method for butter lettuce |
| CN111406631A (en) * | 2020-05-08 | 2020-07-14 | 山东省农业可持续发展研究所 | High-yield and high-quality cultivation method of collard under plant factory conditions |
| CN114375781A (en) * | 2021-04-21 | 2022-04-22 | 北京农学院 | Selenium-rich lettuce hydroponic production method |
| CN114128435A (en) * | 2021-10-21 | 2022-03-04 | 湖南美食流智慧农业科技有限公司 | Selenium-rich lettuce and cultivation method thereof |
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