CN110881333A

CN110881333A - Method for improving organic selenium content of broccoli sprouting vegetable

Info

Publication number: CN110881333A
Application number: CN201911350652.7A
Authority: CN
Inventors: 洪汉君; 肖乾龙
Original assignee: CHENGDU SANHERB BIOTECH CO LTD
Current assignee: CHENGDU SANHERB BIOTECH CO LTD
Priority date: 2019-12-24
Filing date: 2019-12-24
Publication date: 2020-03-17

Abstract

The invention provides a method for improving the organic selenium content of broccoli sprouting vegetables, which comprises the steps of applying exogenous selenium to the broccoli sprouting vegetables under LED illumination, supplementing light for 16 hours by using an LED every day, and keeping the illumination intensity at 50 +/-5 mu mol.m^‑2·s^‑1(ii) a The exogenous selenium is applied to the broccoli sprouting vegetable under different LED illumination conditions, and the method has a remarkable promoting effect on improving the organic selenium content of the broccoli sprouting vegetable.

Description

Method for improving organic selenium content of broccoli sprouting vegetable

Technical Field

The invention relates to a broccoli sprouting vegetable cultivation technology, in particular to a method for improving the organic selenium content of broccoli sprouting vegetable, and belongs to the technical field of cultivation.

Background

Selenium is one of the important vital elements, and proper selenium intake is associated with reducing the risk of prostate cancer, skin cancer, colorectal cancer, liver cancer, breast cancer and lung cancer. Selenium has anticancer, antiaging, antitumor and immunity enhancing effects. If the daily intake of selenium is insufficient, diseases such as osteopathia, keshan disease, cardiovascular and cerebrovascular diseases and the like can be caused.

The broccoli is a brassica plant of cruciferae and contains a large amount of substances beneficial to human health, such as VC, glucosinolates, flavonoids, phenolic substances and the like, wherein the glucosinolates in the broccoli have extremely strong anticancer activity and are easy to hydrolyze under the action of endogenous myrosinase to generate degradation products. The degradation product sulforaphane of 4-methylsulfinylbutylthioglucoside (sulforaphane) is the strongest enzyme inducer discovered so far, and can inactivate oncogene

The broccoli sprout vegetable has fresh, tender, sweet and crisp taste and unique flavor, and has higher content of nutrient substances than the mature inflorescence of broccoli, thereby being deeply loved by people. The broccoli has strong selenium enrichment capacity, can convert selenium in an inorganic form into selenium in an organic form with bioactivity, and plays an important role in human health. A large number of researches show that the application of the exogenous selenium can improve the content of organic selenium in the selenium-rich plants. Light with different light qualities or wavelengths has obviously different biological effects, including plant morphogenesis, photosynthesis, organ growth and development, quality formation and the like. The LED light source is beneficial to the maximization production of vegetable yield, the regulation and control of shape, the optimization of flavor and the synthesis of pigment, and the accumulation of bioactive substances beneficial to human health. Therefore, according to the characteristics of absorbing and accumulating selenium of broccoli sprout vegetables and the special status in human dietary structure, exogenous selenium is utilized to treat the broccoli sprout vegetables under the condition of LED illumination, so that the broccoli sprout vegetables are developed and utilized as selenium-rich foods, and have wider prospects.

Disclosure of Invention

Aiming at the phenomenon, the invention aims to improve the glucosinolate content and the organic selenium content of the broccoli sprouting vegetable and enhance the medicinal and health-care effects of the broccoli sprouting vegetable by carrying out exogenous selenium treatment on the broccoli sprouting vegetable under the illumination of an LED.

The technical scheme of the invention is as follows: a method for improving the content of organic selenium in broccoli sprout vegetables is characterized in that the broccoli sprout vegetables are cultivated under red and blue LED illumination, LED light is supplemented for 16 hours every day, and the illumination intensity is kept at 50 +/-5 mu mol.m^-2·s^-1The red light wavelength is 650-660nm, the blue light wavelength is 450-460nm, and the ratio of the photosynthetic photon density of the red light to the photosynthetic photon density of the blue light is 1:2(1R 2B). In contrast to white light, a 100. mu. mol/L solution of sodium selenite was applied under a white light LED with 1R 2B.

The invention has the beneficial effects that: (1) according to the invention, exogenous selenium is applied to broccoli sprouting vegetables under the LED, so that the method has a remarkable promoting effect on improving the organic selenium content of the broccoli sprouting vegetables; (2) the artificial light quality regulation and control is combined with selenium treatment, so that the contents of glucosinolate and organic selenium in the broccoli are improved and controlled, the market popularization is facilitated, and great economic benefits are generated.

Detailed Description

Example 1

Test method 1

The experiment was carried out at the gardening academy of southern China agricultural university from 11 to 12 months in 2018. Weighing 10g of broccoli seeds with good plumpness, uniform size and no damage, disinfecting the broccoli seeds for 15min by using a 5% sodium hypochlorite solution, soaking the broccoli seeds for 5 hours by using tap water, uniformly spreading the broccoli seeds on a cotton pad after the broccoli seeds fully absorb water and the seed coats fully expand, paving two layers of gauze on the cotton, paving a layer of 35 x 25 filter paper on the cotton pad, and spraying tap water on the seedling tray (the length of the bottom is equal to the length of the bottom, and the width of the bottom is equal to 35 x 25) which is thoroughly soaked. After the cultivation in the shade for 4 days, the plants are respectively placed under different lights to shine, namely white light, white light + selenium and 1R2B + selenium. There were three treatments, each of which was repeated four times. The moisture was kept every day after sowing. 1L of 100 mu mol/L sodium selenite solution is filled at the bottom of each basin, and 10ml of 100 mu mol/L sodium selenite solution is sprayed in the morning and at the evening respectively the next day (white exposure) after the seeds are sowed. The relative humidity in the culture room is controlled at about 80%, the temperature is (25 +/-2) DEG C, and the illumination is 16h d^-1Culturing, sowing, and sampling seven days later.

2 test results and analysis

2.1 Effect of photoplasm and selenium treatment on Broccoli sprout growth

TABLE 1

As can be seen from table 1, the selenium-added treatment resulted in an adverse stress on the growth of broccoli sprout vegetables, and the elongation of hypocotyl of broccoli sprout vegetables was significantly inhibited, compared to white light. The fresh weight of each broccoli sprout treated by selenium under the illumination of 1R2B has no significant difference with that of white light, and the fresh weight of each broccoli sprout treated by the white light selenium treatment is significantly reduced. The water content of broccoli sprout vegetables is reduced by the light-mass ratio and the selenium treatment, and no obvious difference exists in the dry weight of a single plant. The biomass of broccoli sprouting vegetable under different light quality conditions is shown to have strong response to selenium supplement.

2.2 Effect of photoplasm and selenium treatment on Broccoli content in broccoli sprouts

Results of measuring the content of glucosinolates in broccoli sprouting vegetables by HPLC

TABLE 2

As shown in table 2, broccoli sprouting vegetable mainly contains aliphatic glucosinolates. Compared with white light, the content of glucoraphanin, aliphatic glucosinolate, indole glucosinolate and total glucosinolate in broccoli sprout vegetables is respectively reduced by 0.04%, 7.75%, 4.81% and 7.47% through the white light selenium adding treatment, the content of each component of glucosinolate is increased through the selenium adding treatment under the illumination of 1R2B, and the content of glucoraphanin, aliphatic glucosinolate, indole glucosinolate and total glucosinolate is respectively increased by 41.89%, 20.45%, 5.54% and 19.07%.

2.3 Effect of light quality and selenium treatment on selenium content in broccoli sprout

The broccoli sprout has strong selenium enrichment capacity, the organic selenium content of the sprout vegetable can be improved by selenium treatment, wherein the organic selenium content of 1R1B selenium treatment is 1.50 times that of white light selenium treatment.

Claims

1. A method for improving the content of organic selenium in broccoli sprouting vegetables is characterized in that exogenous selenium is applied to the broccoli sprouting vegetables under different LED illumination.

2. The method for increasing the organic selenium content in broccoli sprouts as claimed in claim 1, wherein the seeds are sprayed with 10ml of 100 μmol/L sodium selenite solution in the morning and at the evening (white exposure) the next day after sowing.

3. The method for increasing the organic selenium content of broccoli sprouting vegetables according to claim 1, wherein broccoli sprouting vegetables are cultivated in an LED with a red light to blue light photon density ratio of 1:2(1R2B) and exogenous selenium is applied thereto; wherein the red light wavelength is 650-660nm, and the blue light wavelength is 450-460 nm.