CN107996304A - A kind of temperature adjusting method for improving celery petiole Quantitative Determination of Apigenin - Google Patents
A kind of temperature adjusting method for improving celery petiole Quantitative Determination of Apigenin Download PDFInfo
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- CN107996304A CN107996304A CN201711413432.5A CN201711413432A CN107996304A CN 107996304 A CN107996304 A CN 107996304A CN 201711413432 A CN201711413432 A CN 201711413432A CN 107996304 A CN107996304 A CN 107996304A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/24—Devices or systems for heating, ventilating, regulating temperature, illuminating, or watering, in greenhouses, forcing-frames, or the like
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/25—Greenhouse technology, e.g. cooling systems therefor
Abstract
The present invention relates to a kind of temperature adjusting method for improving celery petiole Quantitative Determination of Apigenin, this temperature adjusting method for improving celery petiole Quantitative Determination of Apigenin：Daily mean temperature be respectively improve under conditions of 12 DEG C and 16 DEG C celery petiole Quantitative Determination of Apigenin day and night temperature control be 12 DEG C, 9 DEG C, 6 DEG C in any one.Improve celery petiole Quantitative Determination of Apigenin daytime temperature/night temperature control be specially：In celery growth early period, 10 ~ 25d after seedling separation, daytime temperature/night temperature control is any one in 22 DEG C/13 DEG C and 23 DEG C/11 DEG C；25 ~ 40d after celery growth mid-term, seedling separation, daytime temperature/night temperature control are any one in 20 DEG C/11 DEG C and 19 DEG C/13 DEG C；In the celery growth later stage, 40 ~ 55d after seedling separation, daytime temperature/night temperature control is any one in 20 DEG C/11 DEG C and 21 DEG C/9 DEG C.The present invention is greatly improved the content of apiolin in celery petiole, improves the quality of celery.
Description
First, technical field
The present invention relates to Installation Vegetable Cultivation field, and in particular to a kind of temperature tune for improving celery petiole Quantitative Determination of Apigenin
Prosecutor method.
2nd, background technology
Celery（Apium graveolensL.）To form 2 years raw herbaceous plant of fertile tender leaf handle in Umbelliferae apium, originate in
Mediterranean, its wild species are thin from the east of Sweden, Algeria, Egypt, Ethiopic humid region to small Asia
Asia, Caucasia, Pakistan, Himalaya Region are all distributed.From ancient Greek, Roman period, its wild species and cultigen
With regard to as medicinal material or spices utilization.China introduces and more early, the ancient book using celery《Tang can want》In be just related to the description of Hu Qin.
Present celery is widely distributed in China, and there are plantation in north and south various regions.Celery belongs to half cold resistance vegetables, and happiness cools, and is adapted to gentle
Weather, with reference to facilities protection measure, can accomplish that the four seasons produce, year-round supply.
Facility cultivation was quickly grown in recent years, it changes the environment such as warm, wet, light, water, fertilizer, gas by various technological means
Condition, relatively controllable or even most suitable environment is provided for plant, therefore breaks the Seasonal Regularity of traditional agriculture production, is realized high
Production, efficient and High-quality Cultivation.Facility cultivation is produced as being decided by two kinds of factors of genotypic and environment, determine cultivar it
Afterwards, there is provided suitable greenhouse just becomes the key of furnished equipments.Many scholars are own through to the warm indoor environment factor and crop
Studied in terms of growth, yield, the relation of quality.Zhang Fu plaster etc. with regard in greenhouse not equality of temperature luminous environment to cucumber photosynthetic generation
Thank, the formation of photosynthate and distribution and law of nutritive absorption etc. have carried out more systematic research.Poplar is strong etc. again to have studied day
Common high and low temperature coerces the influence to greenhouse tomato physiological property in light greenhouse.Luo Xinlan etc. have studied different Ventilation modes
Lower greenhouse tomato Photosynthetic Rate and multiple envirment factors such as temperature, photosynthetically active radiation and CO in facility2Between concentration
Changing rule, establishes tomato cultivation model.
Temperature has facility cultivation crop growth material impact, and the physiology that temperature can significantly affect crop is special
Property, nutritious substances accumulation and distribution and fruit yield, quality etc., the high low and high temperature of temperature or the duration of low temperature and
The day-night change of temperature is all an important factor for influencing crop growth.In the case that average daily temperature is certain, round the clock
The temperature difference（Difference between day and night temperature, DIF）Also crop growth is significantly affected
And quality, day and night temperature are one of the influence form of temperature to plant growth, day and night temperature is to plant growth, endogenous hormones, life
Reason characteristic, yield and quality etc. have a significant impact.
It is the material base of crop organ's formation that assimilation quotient, which is produced with distribution, and day and night temperature adjusts organ " source-storehouse " pass
System and plant forms.Oneself has the experimental results to show, day and night temperature is to various plants such as tomato, cucumber, youth-and-old-age, pimentos
Panel length influences significantly, and most researchers think, growth of the positive DIF to plant panel length, which has, to be promoted to make
With negative DIF then produces inhibitory action to it.Mao Liping has found when studying influence of the day and night temperature to tomato seedling,
Tomato plant height, stem are thick and SL A increases with the increase of day and night temperature.Yang Bo etc. have studied temperature control to non-
The influence of continent chrysanthemum test tube seedling growth, it was demonstrated that the form growth of African Chrysanthemum test tube seedling is optimal during 0 DEG C of day and night temperature processing.Bai Song has found
The stem of intermittent warming group is slightly all higher than control group round the clock, and has with the low and increased trend of night temperature drop.Heuvelink research cards
Real, negative DIF processing can substantially reduce tomato plant stem fresh weight, the number of blade and branch amount than positive DIF processing, lead
Cause blade is thinning, and specific leaf area also significantly reduces.Wu Shu etc. is it has also been found that the wheat not of the same race single plant under 15 DEG C of day and night temperatures is total
Leaf area is larger than what no day and night temperature compareed.
Temperature mainly influences plant strain growth by adjusting Endogenous Hormones, and plant endogenous hormones are more quick to temperature
Sense, endogenous hormones can actively change secretory volume with the change of environment temperature, to adapt to external environment condition.Thingnaes think with
Negative DIF processing is compared, and GA levels drastically reduce under positive DIF in arabidopsis stem, and IAA levels then significantly carry
It is high.Precious strong wait of Zheng carries out day and night temperature experiment to Bowring cattleya, in the newborn blade for finding 25 DEG C/20 DEG C, 30 DEG C/25 DEG C processing
The horizontal rise of GA3, ZR and ABA, IAA contents are reduced；GA3, ZR and ABA are horizontal in the lower blade of 35 DEG C/30 DEG C processing reduces, IAA
Content raises, and hinders bud differentiation.
Photosynthetic metabolite is material base and the energy source of vine growth and development, and day and night temperature can adjust plant photosynthetic organs
Function.Day and night temperature all has an impact the Chlorophyll synthesis of blade, activities ofPSⅡ, the exchange of leaf gas etc..Mao Liping etc. is studied
Show that, when daily mean temperature is 20 DEG C, with the raising of day and night temperature, Nonsto-matal factor limitation declines, and leaf photosynthesis can be to obtain
Improve, add Net Photosynthetic Rate.The result of study such as strong shows poplar again, mean temperature be 18 DEG C when, tomato chlorophyll content with
Day and night temperature has certain positive correlation, and chlorophyll content rise can promote photosynthetic rate to raise to a certain extent.Grape
For blade under positive DIF, RuBP Ayaization enzymatic activitys are higher, and soluble protein and Photosystem I I activity are higher, and with warm round the clock
Difference increase and improve, under negative DIF then photosystem activities reduce.Day and night temperature can also produce plant root vigor
Influence.Xu Panpan has found that tree peony test tube seedling root activity is higher under temperature match curing conditions, constant temperature bar on the research of tree peony test tube seedling
It is relatively low under part；But as day and night temperature increases, root activity is to fall after rising.The researchs such as Zheng Weijie are found, are 3 in day and night temperature
DEG C when oncidiumLuridum test tube seedling form growth it is preferable, its root growth state is optimal when day and night temperature is 12 DEG C.Many research reports
Road, the length of the photosynthesis time of plant growth later stage functional leaf have decisive role to yield composition.Daily mean temperature is 17
DEG C when, tomato seedling dry weight and relative growth rate are higher under 9 DEG C of day and night temperatures, then relatively low under 4 DEG C of day and night temperatures processing.Poplar
The rich result of study waited shows, the organ of African Chrysanthemum test tube seedling is done, fresh weight and day and night temperature are in certain negative correlation, i.e., with
Increasing for day and night temperature and decline.Guo Jin waits research to confirm by force, and day and night temperature increases and is effectively facilitated tamato fruit soluble sugar
Increase with organic acid content.The researchs such as Xiong find that positive DIF has facilitation to the dry matter and sugared content of cucumber.
Shiow and Mary simulates the influence that different day and night temperatures grow strawberry, it is believed that fruit is optimal at 18 DEG C/12 DEG C, whole strain
Grown at 25 DEG C/12 DEG C most suitable.Palace lantern lily flower-shape when day and night temperature is small is preferable, and colored quality is higher.Wen Liangjuan has found
Day and night temperature ambassador's phase tangerine pericarp color is bright-coloured, fruit juice is more, pulp is delicate, flavor is dense, solid content is high.
3rd, the content of the invention
The object of the present invention is to provide a kind of temperature adjusting method for improving celery petiole Quantitative Determination of Apigenin, this raising celery
The temperature adjusting method of petiole Quantitative Determination of Apigenin is used to solve in traditional celery cultural method petiole that Quantitative Determination of Apigenin is low, influences celery
The problem of vegetable matter.
To achieve these goals, the concrete technical scheme that the present invention uses is as follows：This raising celery petiole apiolin
The temperature adjusting method of content：It is respectively under conditions of 12 DEG C and 16 DEG C in daily mean temperature, improves celery petiole Quantitative Determination of Apigenin
Day and night temperature control be 12 DEG C, 9 DEG C, 6 DEG C in any one.
Daytime temperature/night temperature control of raising celery petiole Quantitative Determination of Apigenin is specially in such scheme：In celery growth early period,
10 ~ 25d after seedling separation, daytime temperature/night temperature control are any one in 22 DEG C/13 DEG C and 23 DEG C/11 DEG C；In celery growth mid-term, divide
25 ~ 40d of after seedling, daytime temperature/night temperature control are any one in 20 DEG C/11 DEG C and 19 DEG C/13 DEG C；In the celery growth later stage, seedling separation
40 ~ 55d afterwards, daytime temperature/night temperature control are any one in 20 DEG C/11 DEG C and 21 DEG C/9 DEG C.
Further, the specific control method of daytime temperature/night temperature of raising celery petiole Quantitative Determination of Apigenin is in such scheme：
Celery growth early period, daytime temperature/night temperature control are 22 DEG C/13 DEG C, and in celery growth mid-term, daytime temperature/night temperature control is 20 DEG C/11
DEG C, in the celery growth later stage, daytime temperature/night temperature control is 20 DEG C/11 DEG C.
Further, the specific control method of daytime temperature/night temperature of raising celery petiole Quantitative Determination of Apigenin is in such scheme：
Celery growth early period, daytime temperature/night temperature control are 23 DEG C/11 DEG C, and in celery growth mid-term, daytime temperature/night temperature control is 20 DEG C/11
DEG C, in the celery growth later stage, daytime temperature/night temperature control is 21 DEG C/9 DEG C.
Beneficial effect：
1st, the present invention is by celery accurate temperature regulation, and provides suitable day and night temperature, daytime temperature/night gentle appropriate regulator
In period, be greatly improved the content of apiolin in celery petiole, improves the quality of celery, and apiolin has well anti-oxidant
Property, can be with reducing blood lipid, blood pressure lowering, anticancer, protection maincenter, protection internal organ etc., it has good application in terms of health care
Prospect.
2nd, the present invention quickly develops offer technical guarantee for health celery.
4th, embodiment
The present invention is described further below：
Embodiment 1：
This temperature adjusting method for improving celery petiole Quantitative Determination of Apigenin：It is respectively the condition of 12 DEG C and 16 DEG C in daily mean temperature
Under, the day and night temperature control for improving celery petiole Quantitative Determination of Apigenin is any one in 12 DEG C, 9 DEG C, 6 DEG C.
Embodiment 2：
This temperature adjusting method for improving celery petiole Quantitative Determination of Apigenin：10 ~ 25d after celery growth early period, that is, seedling separation, daytime
Temperature/night temperature control is any one in 22 DEG C/13 DEG C and 23 DEG C/11 DEG C；25 ~ 40d after celery growth mid-term, that is, seedling separation, daytime
Temperature/night temperature control is any one in 20 DEG C/11 DEG C and 19 DEG C/13 DEG C；40 ~ 55d after celery growth later stage, that is, seedling separation, daytime
Temperature/night temperature control is any one in 20 DEG C/11 DEG C and 21 DEG C/9 DEG C.
Embodiment 3：
This temperature adjusting method for improving celery petiole Quantitative Determination of Apigenin：In celery growth early period, daytime temperature/night temperature control is 22
DEG C/13 DEG C, in celery growth mid-term, daytime temperature/night temperature control is 20 DEG C/11 DEG C, in the celery growth later stage, daytime temperature/night temperature control
For 20 DEG C/11 DEG C.
Embodiment 4：
This temperature adjusting method for improving celery petiole Quantitative Determination of Apigenin：In celery growth early period, daytime temperature/night temperature control is 23
DEG C/11 DEG C, in celery growth mid-term, daytime temperature/night temperature control is 20 DEG C/11 DEG C, in the celery growth later stage, daytime temperature/night temperature control
For 21 DEG C/9 DEG C.
In order to verify the effect of the present invention, confidentiality experiment has been carried out, it is specific as follows：
Experiment 1：
For trying celery kind：The crisp celery of glass, is purchased from Shandong Shouguang vegetable seeds Co., Ltd.
On 2 5th, 2016, celery seed soaked 30min sterilizations in 55 DEG C of water, is then dipped in 25 DEG C of water and soaks 24h,
Seedlings nursing plate is planted in after draining（Length×width×height=50cm × 34cm × 12cm）In, seedlings nursing plate is placed on heliogreenhouse
It is interior.The configuration proportion of seedling medium is turf: perlite=1: 3.Nutrient solution in experiment is matched somebody with somebody using Agricultural University Of South China leaf vegetables A
Side, a great number of elements are shown in Table 1, and trace element is shown in Table 2, pH6.4 ~ 7.2.
1 macro-element nutrients formula of liquid of table（mg·L- 1）
2 microelement nutritious liquid formula of table（mg·L- 1）
| Compound name | Na2Fe-EDTA | H3BO3 | MnSO4·4H2O | ZnSO4·7H2O | CuSO4·5H2O | （NH4）6Mo7O24·4H2O |
| Dosage | 20 | 2.86 | 2.13 | 0.22 | 0.08 | 0.02 |
Celery is higher to nutrient demand since increment is big, but especially not resistance to fertilizer, and therefore, the concentration of nutrient solution is controlled not
Scope more than 2.5ms/cm.Just during sowing, nutrient solution concentration will be in the scope of 1.0 ~ 1.5ms/cm, when grow to about 3 ~
After 4 weeks, nutrient solution concentration can be brought up to 1.5 ~ 2.0ms/cm, after growth 2 ~ 3 weeks, nutrient solution concentration can be improved again
To 1.8 ~ 2.5ms/cm, and one week nutrient solution concentration being maintained to before harvesting is not less than 1.8ms/cm, and at this time may not be used
Harvest can be maintained by adding nutrition.
On March 27th, 2016, when seedling is up to 3 leaves, by its seedling separation into 10cm × 10cm nutritive cubes, in nutritive cube still
Turf: perlite=1: 3 matrix is filled, nutrient supply is carried out using Agricultural University Of South China's leaf vegetables A formula nutritionals liquid.By seedling separation
Plant afterwards is transferred in growth cabinet into trip temperature regulation and control and handles.One group of control is equipped with the same time, and moisture and nutrient condition maintain
In same nutrient solution level of supply, adjoining tree is placed in normal growth in experiment greenhouse.
Experiment daily mean temperature is separately designed as 12 DEG C and 16 DEG C, sets 15 DEG C, 12 DEG C, 9 DEG C, 6 DEG C, 3 DEG C and 0 totally six
Day and night temperature, i.e. daytime temperature/night temperature is respectively：28℃/13℃、24℃/9℃、25℃/13℃、21℃/9℃、22℃/13℃、18
DEG C/9 DEG C, 19 DEG C/13 DEG C, 15 DEG C/9 DEG C, 16 DEG C/13 DEG C, 12 DEG C/9 DEG C, 16 DEG C/16 DEG C, 12 DEG C/12 DEG C totally 12 processing, it is real
Temperature is realized within ± 0.5 DEG C in border, each 30 plants of processing, in triplicate.The intensity of illumination of growth cabinet is disposed as
6000Lx, 75% or so, the photoperiod is set as 12h/12h for relative air humidity control（Day night）, thermoperiod is same with the photoperiod
Step, moisture and nutrient condition maintain same nutrient solution level of supply.
55 d concentrate harvest after celery seedling separation, utilize HPLC methods, that is, high effective liquid chromatography for measuring Quantitative Determination of Apigenin.
As shown in table 3：After 55d concentrates harvest after celery seedling separation, CK and based on different daily mean temperatures and day and night temperature base
There is some difference between Quantitative Determination of Apigenin in celery petiole under 12 treatment conditions on plinth.T10, that is, temperature difference is 12 DEG C, daytime
Temperature/night temperature is 21 DEG C/9 DEG C combined treatments, and celery petiole Quantitative Determination of Apigenin highest, it is 9 DEG C, daytime temperature/night temperature with T7, that is, temperature difference
Difference is not notable between 22 DEG C/13 DEG C combined treatments but the two is all remarkably higher than other combined treatments.Secondly, in celery petiole
Quantitative Determination of Apigenin it is higher be the T8 i.e. temperature difference be 9 DEG C and daytime temperature/night temperature be 18 DEG C/9 DEG C combined treatments and the T5 i.e. temperature difference be 6 DEG C and
Daytime temperature/night temperature is 19 DEG C/13 DEG C combined treatments.Under other combined treatments, celery petiole Quantitative Determination of Apigenin is relatively low.
The result shows that compared with CK, T10, T7, T8, T5 combined treatment petiole Quantitative Determination of Apigenin has been respectively increased 11.75%,
10.10%th, 6.89%, 5.67%, illustrate that T10, T7, T8, T5 combined treatment have certain rush to the raising of celery petiole Quantitative Determination of Apigenin
Into effect, show 12 DEG C, 9 DEG C, 6 DEG C of three day and night temperatures improve celery petiole Quantitative Determination of Apigenin and are obviously promoted effect.
Influence of the different temperature difference of table 3 to celery petiole Quantitative Determination of Apigenin
Note：Same column data is compared by order from high to low successively circulation in table, and lowercase letter indication difference is notable after data（P
＜ 0.05）.
Experiment 2：
Test process method is same（One）.When seedling is up to 3 leaves, by its seedling separation into 15cm × 15cm nutritive cubes, in nutritive cube
Still fill turf: perlite=1: 3 matrix, nutrient supply is carried out using Agricultural University Of South China's leaf vegetables A formula nutritionals liquid.Seedling separation
Plant is transferred in growth cabinet into trip temperature regulation and control and handles by 10d afterwards.Each experiment is equipped with one group of control, moisture and nutrient
Condition maintains same nutrient solution level of supply, and adjoining tree is placed in normal growth in experiment greenhouse.
Temperature setting：In result（One）12 DEG C, 9 DEG C, 6 DEG C three screened have celery petiole Quantitative Determination of Apigenin
On the basis of the temperature difference for remarkably promoting effect, daily mean temperature is separately designed as 8 DEG C, 10 DEG C, 12 DEG C, 14 DEG C, 16 DEG C, i.e. daytime temperature/night
Temperature is respectively：17℃/5℃、19℃/7℃、21℃/9℃、23℃/11℃、25℃/13℃、14℃/5℃、16℃/7℃、18
DEG C/9 DEG C, 20 DEG C/11 DEG C, 22 DEG C/13 DEG C, 11 DEG C/5 DEG C, 13 DEG C/7 DEG C, 15 DEG C/9 DEG C, 17 DEG C/11 DEG C, 19 DEG C/13 DEG C totally 15
A processing, studies different daytime temperature/influence of the night temperature to celery petiole Quantitative Determination of Apigenin.
Three experiments are set, study different daytime temperature/night temperature respectively to celery different growing stage petiole Quantitative Determination of Apigenin
Influence.25 d after celery seedling separation of experiment one cut receipts, study different daytime temperature/night temperature to celery growth petiole early period Quantitative Determination of Apigenin
Influence；40 d after celery seedling separation of experiment two cut receipts, study different daytime temperature/night temperature and celery growth mid-term petiole apiolin is contained
The influence of amount；55 d after celery seedling separation of experiment three cut receipts, and research studies different daytime temperature/night temperature to celery growth later stage petiole celery
The influence of dish cellulose content.Control group synchronously cuts receipts with test group.
Influence of the different temperature difference of table 4 to celery growth petiole early period Quantitative Determination of Apigenin
Note：Same column data is compared by order from high to low successively circulation in table, and lowercase letter indication difference is notable after data（P
＜ 0.05）.
As shown in table 4：After celery seedling separation after 25 d harvests, CK and based on being set on the basis of 12 DEG C, 9 DEG C, 6 DEG C of three temperature difference
There is some difference for Quantitative Determination of Apigenin in celery growth petiole early period under the 15 different daytime temperature/night temperature treatment conditions put.T10
I.e. the temperature difference is 9 DEG C and daytime temperature/night temperature is 22 DEG C/13 DEG C combined treatments, and celery petiole Quantitative Determination of Apigenin highest, it is temperature with T14
Difference is 12 DEG C, daytime temperature/night temperature is that difference is not notable between 23 DEG C/11 DEG C combined treatments but the two is all remarkably higher than at other combinations
Reason.Secondly, what Quantitative Determination of Apigenin was higher in celery petiole is that the T13 i.e. temperature difference is 12 DEG C and daytime temperature/night temperature is 21 DEG C/9 DEG C combinations
Processing and T9, that is, temperature difference are 9 DEG C and daytime temperature/night temperature is 20 DEG C/11 DEG C combined treatments.And under remaining 11 combined treatments, leaf of Herba Apii graveolentis
Handle Quantitative Determination of Apigenin is relatively low.The result shows that compared with CK, T10, T14 combined treatment petiole Quantitative Determination of Apigenin are respectively increased
16.35%th, 14.48%, illustrate that T10, T14 combined treatment have certain promotion to the raising of celery growth petiole early period Quantitative Determination of Apigenin
Effect, i.e., 22 DEG C/13 DEG C, 23 DEG C/11 DEG C two daytime temperature/night temperature celery growth petiole early period Quantitative Determination of Apigenin is improved have it is notable
Ground facilitation.
Influence of the different temperature difference of table 5 to celery growth mid-term petiole Quantitative Determination of Apigenin
Note：Same column data is compared by order from high to low successively circulation in table, and lowercase letter indication difference is notable after data（P
＜ 0.05）.
As shown in table 5：After celery seedling separation after 40 d harvests, CK and based on being set on the basis of 12 DEG C, 9 DEG C, 6 DEG C of three temperature difference
There is some difference for Quantitative Determination of Apigenin in celery growth mid-term petiole under the 15 different daytime temperature/night temperature treatment conditions put.T9
I.e. the temperature difference is 9 DEG C and daytime temperature/night temperature is 20 DEG C/11 DEG C combined treatments, celery petiole Quantitative Determination of Apigenin highest, itself and T5, that is, temperature difference
It is that difference is not notable between 19 DEG C/13 DEG C combined treatments but the two is all remarkably higher than at other combinations for 6 DEG C and daytime temperature/night temperature
Reason.Secondly, what Quantitative Determination of Apigenin was higher in celery petiole is that the T10 i.e. temperature difference is 9 DEG C and daytime temperature/night temperature is 22 DEG C/13 DEG C combinations
Processing and T14, that is, temperature difference are 12 DEG C, daytime temperature/night temperature is 23 DEG C/11 DEG C combined treatments.And under remaining 11 combined treatments, celery
Petiole Quantitative Determination of Apigenin is relatively low.The result shows that compared with CK, T9, T5 combined treatment petiole Quantitative Determination of Apigenin are respectively increased
17.80%th, 16.84%, illustrate that T9, T5 combined treatment have certain promotion to make the raising of celery growth mid-term petiole Quantitative Determination of Apigenin
With, i.e., 20 DEG C/11 DEG C, 19 DEG C/13 DEG C two daytime temperature/night temperature to celery growth mid-term petiole Quantitative Determination of Apigenin improve have significantly
Facilitation.
Influence of the different temperature difference of table 6 to celery growth later stage petiole Quantitative Determination of Apigenin
Note：Same column data is compared by order from high to low successively circulation in table, and lowercase letter indication difference is notable after data（P
＜ 0.05）.
As shown in table 6：After celery seedling separation after 55 d harvests, CK and based on being set on the basis of 12 DEG C, 9 DEG C, 6 DEG C of three temperature difference
There is some difference for Quantitative Determination of Apigenin in celery growth later stage petiole under the 15 different daytime temperature/night temperature treatment conditions put.T9
I.e. the temperature difference be 9 DEG C, daytime temperature/night temperature be 20 DEG C/11 DEG C combined treatments, celery petiole Quantitative Determination of Apigenin highest, itself and T13, that is, temperature difference
It is that difference is not notable between 21 DEG C/9 DEG C combined treatments but the two is all remarkably higher than at other combinations for 12 DEG C and daytime temperature/night temperature
Reason.Secondly, what Quantitative Determination of Apigenin was higher in celery petiole is that the T10 i.e. temperature difference is 9 DEG C and daytime temperature/night temperature is 22 DEG C/13 DEG C combinations
Processing.And under remaining 12 combined treatments, celery petiole Quantitative Determination of Apigenin is relatively low.The result shows that compared with CK, T9, T13 group
Close processing petiole Quantitative Determination of Apigenin and be respectively increased 14.50%, 13.11%, illustrate T9, T13 combined treatment to celery petiole celery
Cellulose content, which improves, certain facilitation, i.e., 20 DEG C/11 DEG C, 21 DEG C/9 DEG C two daytime temperature/night temperature are to celery growth later stage leaf
Handle Quantitative Determination of Apigenin improves and is obviously promoted effect.
Experiment 3：
In result（Two）What is screened has celery different growing stage petiole Quantitative Determination of Apigenin the daytime for the effect that remarkably promotes
Temperature/night temperature, result is respectively adopted in 10 d after celery growth early period, that is, seedling separation（Two）Screen to celery growth leaf early period
Quantitative Determination of Apigenin has two kinds of suitable daytime temperature/night temperature of facilitation in handle（I.e. 22 DEG C/13 DEG C and 23 DEG C/11 DEG C）Into trip temperature
Result is respectively adopted in 25 d after regulation and control, growth medium, that is, seedling separation（Two）Screen to apiolin in celery growth mid-term petiole
Content has two kinds of suitable daytime temperature/night temperature of facilitation（I.e. 20 DEG C/11 DEG C and 19 DEG C/13 DEG C）Into trip temperature regulation and control, growth
Result is respectively adopted in 40 d after later stage, that is, seedling separation（Two）What is screened has Quantitative Determination of Apigenin in celery growth later stage petiole
The suitable daytime temperature/night temperature of two kinds of facilitation（I.e. 20 DEG C/11 DEG C and 21 DEG C/9 DEG C）Regulate and control into trip temperature, the 55 d collection after seedling separation
Middle harvesting, studies different suitable daytime temperature/influence of night temperature combination to celery petiole Quantitative Determination of Apigenin.One group of control is equipped with the same time,
Moisture and nutrient condition maintain same nutrient solution level of supply, and adjoining tree is placed in normal growth in experiment greenhouse.
7 different growing stage of table difference daytime temperature/night temperature combines the influence to celery petiole Quantitative Determination of Apigenin
As shown in table 7：Result is respectively adopted in celery growth early period, growth medium, Later growth（Two）Screen to celery
Suitable daytime temperature/night temperature that Quantitative Determination of Apigenin has facilitation in dish growth different times petiole regulates and controls into trip temperature, it is combined
Respectively handle that there is some difference to Quantitative Determination of Apigenin in celery petiole with CK afterwards.CK is substantially less than each combined treatment, shows each
Combined treatment, which improves celery petiole Quantitative Determination of Apigenin, has certain promotion, and Quantitative Determination of Apigenin increase rate is 12.03%
Between ~ 27.61%.Processing 1 i.e. growth uses 22 DEG C/13 DEG C early period（Daytime temperature/night temperature）+ growth medium uses 20 DEG C/11 DEG C（Daytime
Temperature/night temperature）+ Later growth uses 20 DEG C/11 DEG C（Daytime temperature/night temperature）23 DEG C/11 DEG C are used with processing 6 i.e. growth early period（Daytime
Temperature/night temperature）+ growth medium uses 20 DEG C/11 DEG C（Daytime temperature/night temperature）+ Later growth uses 21 DEG C/9 DEG C（Daytime temperature/night temperature）It is right
Quantitative Determination of Apigenin facilitation is most notable in celery petiole, improves 27.61% and 24.63% than CK respectively, be significantly higher than other 6
A processing and CK, are more satisfactory combined treatments.
Above-mentioned as shown by data, there is some difference for celery petiole Quantitative Determination of Apigenin under temperature difference control technique.
First, there is some difference to celery petiole Quantitative Determination of Apigenin for different day and night temperature control techniques.It is suitably warm round the clock
Poor level is 12 DEG C, 9 DEG C and 6 DEG C, and celery petiole Quantitative Determination of Apigenin is significantly improved under the day and night temperature.
Secondly, there is celery different growing stage petiole Quantitative Determination of Apigenin certain poor in different daytime temperature/night temperature control technique
It is different.Suitable celery growth early period（10 ~ 25 d i.e. after seedling separation）Daytime temperature/night temperature is 22 DEG C/13 DEG C and 23 DEG C/11 DEG C, the daytime temperature/night
Celery growth petiole early period Quantitative Determination of Apigenin is significantly improved under temperature；Suitable celery growth mid-term（25 ~ 40 d i.e. after seedling separation）Daytime
Temperature/night temperature is 20 DEG C/11 DEG C and 19 DEG C/13 DEG C, and significantly improving celery growth mid-term petiole apiolin under the daytime temperature/night temperature contains
Amount；The suitable celery growth later stage（40 ~ 55 d i.e. after seedling separation）Daytime temperature/night temperature is 20 DEG C/11 DEG C and 21 DEG C/9 DEG C, the daytime temperature/night
Celery growth later stage petiole Quantitative Determination of Apigenin is significantly improved under temperature.
Finally, celery different growing stage uses different daytime temperature/night temperature combinatorial regulation to Quantitative Determination of Apigenin in celery petiole
There is some difference.Temperature control technique uses 22 DEG C/13 DEG C early period for growth（Daytime temperature/night temperature）+ growth medium uses 20
℃/11℃（Daytime temperature/night temperature）+ Later growth uses 20 DEG C/11 DEG C（Daytime temperature/night temperature）23 DEG C/11 DEG C are used with growth early period
（Daytime temperature/night temperature）+ growth medium uses 20 DEG C/11 DEG C（Daytime temperature/night temperature）+ Later growth uses 21 DEG C/9 DEG C（Daytime temperature/night
Temperature）, Quantitative Determination of Apigenin in celery petiole is significantly improved under the combination condition.
Therefore, in celery production, to improve celery petiole Quantitative Determination of Apigenin, temperature control technique correct method is celery
Grow and use 22 DEG C/13 DEG C early period（Daytime temperature/night temperature）+ growth medium uses 20 DEG C/11 DEG C（Daytime temperature/night temperature）+ Later growth
Using 20 DEG C/11 DEG C（Daytime temperature/night temperature）23 DEG C/11 DEG C are used with growth early period（Daytime temperature/night temperature）+ growth medium using 20 DEG C/
11℃（Daytime temperature/night temperature）+ Later growth uses 21 DEG C/9 DEG C（Daytime temperature/night temperature）It is adapted to the most.
Celery is a kind of health-care vegetable for eating medicine dual-purpose, and the apiolin in celery has good inoxidizability, can drop
Blood fat, blood pressure lowering, anticancer, protection maincenter, protection internal organ etc., it has a good application prospect in terms of health care, positive day
Benefit causes the common concern of researcher.The intelligent Cultivation in Greenhouse area in China is progressively expanding at present, has huge
Development potentiality, the technology of temperature regulation and control plant growth is also being continuously improved, and vegetable crop is improved by varying day and night temperature
Yield and quality, be significant research direction.Temperature can regulate and control growth and development and the yield composition of celery round the clock, to celery
Vegetable matter, which includes Quantitative Determination of Apigenin, has certain influence.Therefore, regulate and control in the production of health celery using this kind of temperature
Technology, it is possible to increase celery petiole Quantitative Determination of Apigenin.
Claims (4)
- A kind of 1. temperature adjusting method for improving celery petiole Quantitative Determination of Apigenin, it is characterised in that：It is respectively 12 in daily mean temperature DEG C and 16 DEG C under conditions of, improve celery petiole Quantitative Determination of Apigenin day and night temperature control be 12 DEG C, 9 DEG C, 6 DEG C in it is any one It is a.
- 2. the temperature adjusting method according to claim 1 for improving celery petiole Quantitative Determination of Apigenin, it is characterised in that：It is described Temperature be regulated to daytime temperature/night temperature, in celery growth early period, 10 ~ 25d after seedling separation, daytime temperature/night temperature control for 22 DEG C/13 DEG C and Any one in 23 DEG C/11 DEG C；25 ~ 40d after celery growth mid-term, seedling separation, daytime temperature/night temperature control are 20 DEG C/11 DEG C and 19 DEG C/13 DEG C in any one；In the celery growth later stage, 40 ~ 55d after seedling separation, daytime temperature/night temperature control for 20 DEG C/11 DEG C and 21 DEG C/ Any one in 9 DEG C.
- 3. the temperature adjusting method according to claim 2 for improving celery petiole Quantitative Determination of Apigenin, it is characterised in that：It is described Temperature be regulated to daytime temperature/night temperature, in celery growth early period, daytime temperature/night temperature control is 22 DEG C/13 DEG C, in celery growth mid-term, Daytime temperature/night temperature control is 20 DEG C/11 DEG C, and in the celery growth later stage, daytime temperature/night temperature control is 20 DEG C/11 DEG C.
- 4. the temperature adjusting method according to claim 2 for improving celery petiole Quantitative Determination of Apigenin, it is characterised in that：It is described Temperature be regulated to daytime temperature/night temperature, in celery growth early period, daytime temperature/night temperature control is 23 DEG C/11 DEG C, in celery growth mid-term, Daytime temperature/night temperature control is 20 DEG C/11 DEG C, and in the celery growth later stage, daytime temperature/night temperature control is 21 DEG C/9 DEG C.
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| CN113079785A (en) * | 2021-04-13 | 2021-07-09 | 黑龙江八一农垦大学 | Microelement application method for improving celery petiole apigenin content |
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| CN113079785A (en) * | 2021-04-13 | 2021-07-09 | 黑龙江八一农垦大学 | Microelement application method for improving celery petiole apigenin content |
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