CN109122264A - A kind of method that additional uv b radiation improves Greenhouse Water Culture romaine lettuce Flavonoid Content - Google Patents
A kind of method that additional uv b radiation improves Greenhouse Water Culture romaine lettuce Flavonoid Content Download PDFInfo
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- CN109122264A CN109122264A CN201810964530.6A CN201810964530A CN109122264A CN 109122264 A CN109122264 A CN 109122264A CN 201810964530 A CN201810964530 A CN 201810964530A CN 109122264 A CN109122264 A CN 109122264A
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- romaine lettuce
- radiation
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- flavonoid content
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/14—Measures for saving energy, e.g. in green houses
Abstract
The invention discloses a kind of methods that additional uv b radiation improves Greenhouse Water Culture romaine lettuce Flavonoid Content to sterilize method includes the following steps: taking romaine lettuce seed, is placed in on the 1/2 suddenly gauze of Glan nutrient solution infiltration；Gauze and romaine lettuce seed are placed in vessel, with ventilative preservative film sealing with holes, culture under 15-20 DEG C of temperature, white light is placed in extremely to germinate, use 1/2 Glan culture solution water planting 2-3 weeks suddenly after being cultivated for 5-7 days instead, then uv b radiation 3-4h is added to romaine lettuce young plant daily, so after 6-8 days, romaine lettuce is harvested.Method of the invention can be such that the Flavonoid Content of Greenhouse Water Culture romaine lettuce significantly improves and keep stable, wherein Flavonoid Content increases 30% or more after uv b radiation 6 days, increase the nutritional quality of romaine lettuce, and do not inhibit Growth of Lettuce, biomass is unaffected.
Description
Technical field
The invention belongs to ecological agriculture production fields, and in particular to a kind of additional uv b radiation raising Greenhouse Water Culture lettuce class
The method of flavones content.
Background technique
Romaine lettuce (Lactuca sativa L) is being commonly called as Leaf lettuce, belongs to composite family Lactuca, is annual or biennial
Herbaceous plant, leaf long inverted ovoid, intensively at wild cabbage shape leaf-head；Rich in vitamin C, vitamin E, carotenoid and Polyphenols
Antioxidant can be eaten raw, be the most frequently used vegetable variety for making salad, it is considered to be the most popular healthy vegetable of China and foreign countries' cuisines
One of dish.
Romaine lettuce has effects that clear liver and gallbladder, heat clearing and tranquillizing, nourishing the stomach.It is suitable for slimmer, is suitable for high cholesterol, neurasthenia
Person, liver and bladder disease and patients with gastric disease are edible.Lettuce element in cauline leaf also ease pain hypnosis, reduce cholesterol the effects of, can be used for
Calm and treatment insomnia, the ingredients such as mannitol can promote blood circulation, and play the role of diuresis.
Currently, the appearance of Greenhouse Water Culture vegetables technology well solves since soil and heavy metal pollution of water body are serious
This problem, and solve the problems, such as that land utilization ratio is limited, while also can in extreme regions such as cold, desert, islands
Fresh vegetables is produced, reduces the cost and exhaust gas discharge of other places long-distance transport vegetables, this is a kind of production method of low-carbon environment-friendly.
But the artificial light source on usual Greenhouse Water Culture shelf is single, is all cool white light and warm yellow light, does not meet the institute of nature light
There is ingredient, seriously affects vegetable yields and quality.
Sunlight includes ultraviolet light, visible light and infrared ray, and the ultraviolet radioactive in nature is divided into according to wave band difference
Three kinds, UV-A (315-400nm), UV-B (280-315nm) and UV-C (200-280nm), UV-C and big portion in solar radiation
UV-B is divided to be absorbed by ozone layer without reaching earth surface, only UV-A and a small amount of UV-B eventually arrive at earth surface, although
UV-B content in sunlight is not high, but the UV-B in sunlight plays indispensable role to plant growth and development.
A large amount of polyphenol substances, which can be generated, after ultraviolet light in plant reception sunlight protects itself.Plant is under uv b radiation
The metabolite of generation not only assists in plant and resists adverse circumstance, insect pest, moreover it is possible to improve the nutritive value of many food plants.Institute
To be a kind of scientific and effective method by light source, light quality regulation improvement greenhouse luminous environment, have to greenhouse vegetable production development
Positive effect, using becoming increasingly popular.
Flavonoids is a kind of Polyphenols health substance, generally existing in various veterinary antibiotics.Flavonoids can be divided into Huang
Keto-alcohol, flavonoids, anthocyan, flavanone, -3 alcohols of flavane and isoflavones element are formed after plant receives UV-B signal
A kind of secondary metabolite.Flavonoids is not only the important metabolite that plant resists adverse circumstance, and some researches show that people in recent years
Class is related to human diseases to the absorption of flavonoids, such as the prevention of cancer, pulmonary disease.Mankind itself cannot synthesize class Huang
Ketone, therefore vegetable and fruit is the important sources of human consumption's flavonoids.
Summary of the invention
The purpose of the present invention is to provide a kind of method that additional uv b radiation improves Greenhouse Water Culture romaine lettuce Flavonoid Content,
This method can be such that the nutritional quality of Greenhouse Water Culture romaine lettuce is improved.
The purpose of the invention is achieved by the following technical solution:
A kind of method that additional uv b radiation improves Greenhouse Water Culture romaine lettuce Flavonoid Content, comprising the following steps:
Romaine lettuce seed is taken, is sterilized, is placed in on the 1/2 suddenly gauze of Glan nutrient solution infiltration, gauze and romaine lettuce seed is set
In vessel, sealed with ventilative preservative film with holes；It is placed in culture under 15-20 DEG C of temperature, white light and is cultivated for 5- to germinateing
It uses 1/2 Glan culture solution water planting 2-3 weeks suddenly after 7 days instead, uv b radiation 3-4h, such 6-8 then is added to romaine lettuce young plant daily
After it, Flavonoid Content is significantly increased in romaine lettuce, at this moment can harvest romaine lettuce；
The romaine lettuce seed disinfection is preferably sterilized with 30% liquor natrii hypochloritis；
The Glan nutrient solution suddenly of described 1/2, formula is FH2PO4136g/L, KNO3101g/L, MgSO4246.5g/L
MnCl·4H2O 1.81g/L, H2BO32.86g/L CuSO4·5H2O 0.08g/L, ZnSO40.22g/L, H2MnO4·H2O
0.02g/L, Ca (NO4)2236g/L, Fe-EDTA 2.78g/L；
The gauze and vessel is also all sterilized with 30% hydrogenperoxide steam generator in advance；
The uv b radiation can be carried out continuously, and can also be interrupted progress, intensity 0.6-0.8Wm-2；
Preferably, the uv b radiation is carried out continuously between daily 11:30-14:30.This is the biology of simulating plant
Clock, natural conditions lower high noon are the sunlight middle-ultraviolet lamp strongest moment.Meanwhile 3 hours uv b radiation intensity reaches far away high
Dosage UV-B stress, not will cause DNA damage, will not influence Growth of Lettuce.Moderately adding uv b radiation can overcome the disadvantages that greenhouse water
Training romaine lettuce receives the disadvantage of sunlight deficiency.
The present invention has the following advantages and effects with respect to the prior art:
1, method of the invention can be such that the Flavonoid Content of Greenhouse Water Culture romaine lettuce significantly improves and keep stable, wherein
Flavonoid Content increases 30% or more after uv b radiation 6 days, increases the nutritional quality of romaine lettuce, and does not inhibit Growth of Lettuce,
Biomass is unaffected.
2, the technology of the present invention conclusion can provide selection additional time, light intensity for the greenhouse production artificially feed technology of romaine lettuce
Reference data also provides fundamental basis for the further exploitation of agricultural production source material, improves industrialized agriculture luminous environment.
Detailed description of the invention
Fig. 1 is romaine lettuce in additional UV-B treated fresh weight change curve.
Fig. 2 is the change curve of romaine lettuce Flavonoid Content after additional UV-B processing.
Specific embodiment
Present invention will now be described in further detail with reference to the embodiments and the accompanying drawings, but embodiments of the present invention are unlimited
In this.
In following embodiment, the measurement of romaine lettuce blade Flavonoid Content uses following methods:
Using Flavonoid Content in spectrophotometer method measurement romaine lettuce blade.The fresh romaine lettuce blade of blade 0.1g is weighed, is added
Enter 1% acidic alcohol of 1ml, proof press, which is smashed, is placed on 30 DEG C, and 13000g is centrifuged after 2h is extracted in 100rpm/min shaking table
10min, takes 0.9ml extracting solution, and control takes 1% acidic alcohol of 0.9ml to sequentially add 5% and 10%, add every time shake up it is rear quiet
6min is set, is eventually adding after 4%NaOH solution shakes up and stands 15min, 2ml prepare liquid is taken to measure OD520.Standard curve checks in class
Flavonoid concentration simultaneously calculates content.
Statistical analysis uses following steps:
It is for statistical analysis using software SPSS13.6.It is analyzed with one-way analysis of variance (ANOVA) as a result, using
Tekey ' s Multiple range test detects the difference between average value, and significant difference is defined as the otherness of p < 0.05 to be indicated with *；And it is aobvious
It writes difference and is defined as < 0.01, the opposite sex indicates that all data are expressed as line chart with * *.
Embodiment
A kind of method that additional uv b radiation improves Greenhouse Water Culture romaine lettuce Flavonoid Content, comprising the following steps:
Romaine lettuce seed is taken, 5min is sterilized with 30% liquor natrii hypochloritis, decontamination liquid is cleaned with pure water, with 30% peroxidating
Hydrogen solution sterilizes glass culture dish and gauze and uses clear water washing and sterilizing liquid, and gauze is layered on culture dish bottom and the lattice suddenly of addition 1/2
Blue culture solution wetting, romaine lettuce seed is uniformly laid on gauze, is placed under white light and is cultivated 7 days with the preservative film sealing of Zha Dong,
It is transferred to containing being cultivated 2 weeks in the 1/2 suddenly hydroponic device of Glan culture solution, the daily 11:30-14:30 in 8 days before harvesting is additional
0.6-0.8W·m-2UV-B takes romaine lettuce the 3rd and the 4th pair of crop leaf measuring Flavonoid Content in every two days.
UV-B fluorescent tube is placed in parallel above romaine lettuce, and measuring its radiation intensity with UV radiation intensity instrument is 0.6-0.8Wm-2, a sample is taken within every two days, wherein adding UV-B with nothing under the same treatment time is control, the result is shown in Figure 1 and Fig. 2.
Adding low dosage UV-B in Greenhouse Water Culture romaine lettuce production process it can be seen from Fig. 1 and Fig. 2 can induce romaine lettuce conjunction
At more flavonoids, the nutrient composition content of romaine lettuce is improved, solves the deficiency that Greenhouse Water Culture romaine lettuce receives less UV-B；
Meanwhile the accumulation of biomass will not be reduced because of additional low dosage UV-B in vegetables, and the increase of romaine lettuce yield is made to be maintained at phase
In the state of stabilization, guarantee to improve romaine lettuce nutritional quality while romaine lettuce yield.Wherein, UV-B flavonoids after the 4th day is added
Content is obviously increased compared with without additional UV-B, and the 4th day lettuce class flavones content increases 25-26%, increases 37-38% within the 6th day.
At the same time, UV-B does not impact the biomass of romaine lettuce, and romaine lettuce yield grows steadily.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (7)
1. a kind of method that additional uv b radiation improves Greenhouse Water Culture romaine lettuce Flavonoid Content, it is characterised in that including following step
It is rapid:
Romaine lettuce seed is taken, is sterilized, is placed in on the 1/2 suddenly gauze of Glan nutrient solution infiltration；Gauze and romaine lettuce seed are placed in device
It in ware, is sealed with ventilative preservative film with holes, is placed in culture under 15-20 DEG C of temperature, white light and is cultivated for 5-7 days to germinateing
After use 1/2 Glan culture solution water planting 2-3 weeks suddenly instead, uv b radiation 3-4h then added to romaine lettuce young plant daily, such 6-8 days
Afterwards, romaine lettuce is harvested.
2. according to the method described in claim 1, it is characterized by: the uv b radiation, is carried out continuously, or interruption carries out.
3. according to the method described in claim 1, it is characterized by: the uv b radiation, intensity 0.6-0.8Wm-2。
4. according to the method described in claim 1, it is characterized by: the uv b radiation, connects between daily 11:30-14:30
It is continuous to carry out.
5. according to the method described in claim 1, it is characterized by: the romaine lettuce seed disinfection, molten with 30% sodium hypochlorite
Liquid disinfectant.
6. according to the method described in claim 1, it is characterized by: the gauze and vessel, preparatory 30% hydrogen peroxide
Solution disinfection.
7. according to the method described in claim 1, it is characterized by: described 1/2 Glan nutrient solution suddenly, formula is FH2PO4
136g/L, KNO3101g/L, MgSO4246.5g/L, MnCl4H2O 1.81g/L, H2BO32.86g/L CuSO4·5H2O
0.08g/L, ZnSO40.22g/L, H2MnO4·H2O 0.02g/L, Ca (NO4)2236g/L, Fe-EDTA 2.78g/L.
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| CN201810964530.6A CN109122264A (en) | 2018-08-23 | 2018-08-23 | A kind of method that additional uv b radiation improves Greenhouse Water Culture romaine lettuce Flavonoid Content |
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| CN201810964530.6A CN109122264A (en) | 2018-08-23 | 2018-08-23 | A kind of method that additional uv b radiation improves Greenhouse Water Culture romaine lettuce Flavonoid Content |
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| CN110249921A (en) * | 2019-06-27 | 2019-09-20 | 浙江海洋大学 | Improve the breeding method of flavonoids and aldehydes matter content in Farfugium japonicum |
| CN111418380A (en) * | 2020-04-15 | 2020-07-17 | 厦门大学 | Illumination culture method for promoting green stalk vegetable and four-nine heart vegetable to increase green |
| CN115177648A (en) * | 2022-08-24 | 2022-10-14 | 暨南大学 | Method for rapidly promoting accumulation of flavones and selenium-rich conversion in picked chrysanthemum leaves and application |
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| CN111418380A (en) * | 2020-04-15 | 2020-07-17 | 厦门大学 | Illumination culture method for promoting green stalk vegetable and four-nine heart vegetable to increase green |
| CN115177648A (en) * | 2022-08-24 | 2022-10-14 | 暨南大学 | Method for rapidly promoting accumulation of flavones and selenium-rich conversion in picked chrysanthemum leaves and application |
| CN115177648B (en) * | 2022-08-24 | 2023-11-24 | 暨南大学 | Method for rapidly promoting accumulation of flavonoids in picked chrysanthemum leaves and selenium-enriched conversion and application |
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