CN103931429A - Artificial light seedling culture method capable of promoting plant growth and differentiation - Google Patents

Artificial light seedling culture method capable of promoting plant growth and differentiation Download PDF

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Publication number
CN103931429A
CN103931429A CN201410094091.XA CN201410094091A CN103931429A CN 103931429 A CN103931429 A CN 103931429A CN 201410094091 A CN201410094091 A CN 201410094091A CN 103931429 A CN103931429 A CN 103931429A
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differentiation
plant growth
light
growth
promoting plant
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刘珂岑
李超
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YUNNAN YUNQIDAN BIOLOGICAL SCIENCE & TECHNOLOGY Co Ltd
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YUNNAN YUNQIDAN BIOLOGICAL SCIENCE & TECHNOLOGY Co Ltd
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/14Measures for saving energy, e.g. in green houses

Abstract

The invention discloses an artificial light seedling culture method capable of promoting plant growth and differentiation. In the plant growth and differentiation periods, LED SMD lamps are used as light sources, and the light wavelength is controlled and maintained within the range from 610 nm to 660 nm. According to the method, by combining natural light irradiation and enhancing radiation of the specific spectroscopic light source at specific time, performance of plants in photosynthesis dark reaction is enhanced, and therefore the quality of crops is improved, the yield of products is increased, and residual harmful chemicals are reduced to the maximum degree; by the adoption of the high-quality LED SMD lamps used as the light sources, only deep red light of which the wavelength is about 660 nm is maintained, crops are irradiated at the critical crop growth periods (the growth period and the differentiation period), so that the growth and differentiation speed is effectively improved, quality of the crops is improved, economic benefits are increased, the residual harmful chemicals are avoided, and the deep red light can be seen as plant growth light fertilizer.

Description

The artificial light seedling cultivation method of a kind of Promoting plant growth and differentiation
Technical field
The invention belongs to biological technical field, be specifically related to the artificial light seedling cultivation method of a kind of Promoting plant growth and differentiation.
Background technology
At present, aspect the group training of draft seedling that has higher economic worth, what take is all natural daylight.According to the printing opacity illumination of the natural lighting in natural wild country and booth inside, can bring different impacts to the growth of seedling after group training.No matter but which kind of existing illumination condition, all cannot be promoting growth rate, improve growth quality, reduce chemical substance and bring breakthrough aspect residual.This has caused serious obstacle to economic benefit and product quality aspect for the crop later stage with higher economic worth.
For instance, existing market focus product-stem of noble dendrobium from tissue differentiation to transplanting for the first time, just need to expend the time of at least 8 months in sterilization differentiation bottle.In order to improve the growth rate of plant, the method that can't take chemical composition to get involved.So, greatly delay the product speed of crop, be unfavorable for the popularization of high economic worth crop, also affected greatly the process of industrialization and the economic benefit in later stage.Therefore, develop and a kind ofly can promote plant growth rate, improve growth quality, reduce chemical substance residual seedling-cultivating method and be very important.
Summary of the invention
The object of the present invention is to provide the artificial light seedling cultivation method of a kind of Promoting plant growth and differentiation.
The object of the present invention is achieved like this, at plant growth and in the idiophase, adopts LED SMD lamp as light source, and controlling and keeping optical wavelength is 610 ~ 660nm.
The present invention is in conjunction with natural light irradiation, strengthening special spectrum light source is in the radiation of special time, the performance of fortification of plants in photosynthesis dark reaction, thereby promote crop quality and output, reduce to greatest extent the residual of harmful chemical, adopt high-quality LED paster light source, only retain the dark red coloured light of 660nm left and right, in (the growth of plant growth key period, idiophase) crop is irradiated, thereby effectively promote Growth and Differentiation speed, improve crop quality, increase economic efficiency, avoid the residual of harmful chemical simultaneously, can be considered as " the plant growth light fertilizer " of novelty.
Brief description of the drawings
Fig. 1 is the spatial distribution schematic diagram of each coloured light;
Fig. 2 is peculiar spectrum light source of the present invention and the natural daylight radiation response schematic diagram for crop;
Fig. 3 is the application scenarios of the present invention in part producing place.
Embodiment
Below in conjunction with accompanying drawing, the present invention is further illustrated, but never in any form the present invention is limited, and any conversion or the replacement done based on training centre of the present invention, all belong to protection scope of the present invention.
Promoting plant growth under the present invention and the artificial light seedling cultivation method of differentiation are at plant growth and in the idiophase, adopt LED SMD lamp as light source, and controlling and keeping optical wavelength is 610 ~ 660nm.
Described plant is for need to carry out photosynthetic plant.
Described plant is the one in the stem of noble dendrobium, cucumber, tomato, capsicum, potato, eggplant, konjaku, celery, asparagus, fresh kidney beans, onion, cabbage mustard, tulip, hyacinth, chrysanthemum, rose, Chinese rose.
The irradiation time of described light source is 14 ~ 18h.
Described irradiation time is 16h.
Described light source irradiation time is that the front 7 ~ 8h of sunset time is to post sunset 7 ~ 8h.
Described optical wavelength is 660nm.
The growth of plant, all be unable to do without photosynthesis.Effectively photosynthesis, can Promoting plant growth.Process, the intervention that can not have any chemically grown promotion composition simultaneously and residual broken up in order effectively to accelerate plant growth, we have created the method for " utilizing artificial visible ray particular frequency bands spectrum to grow seedlings ".
Natural daylight is a complete spectrum, and wherein 660nm's is dark red only to the plant differentiation growth light source of the most effectively growing.From the angle of light source reliability and cost control, we take high-quality LED paster as light source simultaneously, in the ruddiness region of 610-660nm, and electricity conversion the highest (expectation reaches 42%).What is more important, in irradiation process, light source can not produce uncontrollable thermal source, otherwise can cause crushing blow to plant growth, also be unfavorable for energy-saving and emission-reduction, therefore, our selected LED light source paster shows very good aspect heat endurance, and logical light quantity when joint temperature 85 is spent has reached 93% left and right.
The invention process technique and application method: we adopt such innovation with the bottled seedling of the stem of noble dendrobium.Utilize 660nm ruddiness, do not affecting on the basis of photosynthesis of plant dark reaction the photosynthetic photoreaction of fortification of plants.Due to the bottled seedling that stem of noble dendrobium seedling is sterilizing bottle, we put bottled seedling at shelf higher slice.German Ou Silang LH CPDP LED light source paster (only producing the dark red light of 660nm) is processed into LED light belt by we, replaces original high-pressure sodium light modulation using in green house, and two light belts are installed on every layer of shelf, irradiates every day 16 hours.The selection of irradiation time is that sunset time arrives post sunset 8 hours in first 8 hours, thereby in without natural daylight, also can allow the photosynthesis of plant be strengthened.
The validity of irradiating in order to reach light source, has installed the light screening material that shields external light source on our the creationary shelf testing.Can be improved to if desired spectrum seedling growing box, seedling growing box is easy to manufacture on a large scale, and cost is easy to control.
Range of application: everyly need to carry out photosynthetic plant, in initial vegetative period, can utilize this technique and technology.Economize greatly as green in Yunnan, possesses application space widely at flowers, economic crops, specialized species aspect growing seedlings.Due to the use of this technology and technique, can on cost, bring certain pressure in the early stage, but possess very strong advantage at aspects such as standing cost control and economic benefit liftings.Therefore we advise that this technology and technique should be applied to new species cultivation and have the cultivation industrialized development of the crop of higher economic worth.
Advantage of the present invention:
1, accelerate the growth rate of crop in group training booth.Taking stem of noble dendrobium seedling as example, due to the employing of this technology and technique, shorten the growth time of 20% left and right;
2, improve quality, improve output: because photosynthesis is abundant, under the constant condition of other conditions, crop alimentary material fully ensured, therefore the quality of crop plant is significantly improved.Taking stem of noble dendrobium seedling as example, seedling rooting rate improves 6%(92-98%), transplanting succeed rate promotes 11%(85-96%), seedling later stage infection rate decline 3%(8-5%).Taking cucumber growth as example, the enforcement of this technology and technique can realize volume increase 20%, and quality and appearance are obviously promoted, and Growth and yield is effectively extended season simultaneously;
3,, owing to having substituted original artificial light source, as the high-pressure mercury lamp that greenhouse, booth generally adopt, save the energy 60%.Because LED belongs to low temperature light source, also improve the safety of production process;
4,, owing to having adopted this innovative technology and technique, at this main growing period of crop seedling phase, by no longer adopting any plant growth promoter, therefore avoided to greatest extent the residual of harmful chemical component.
Operation principle of the present invention: the thinking of this technology is in conjunction with natural light irradiation, strengthens the irradiation of special spectrum light source at special time, the performance of fortification of plants in photosynthesis dark reaction, thereby promote crop quality and output, reduce to greatest extent the residual of harmful chemical.Adopt high-quality LED paster light source, only retain the dark red coloured light of 660nm left and right, in the plant growth key period, crop is irradiated, thus effectively promote Growth and Differentiation speed, improve crop quality, increase economic efficiency, avoid the residual of harmful chemical simultaneously.Can be considered as " the plant growth light fertilizer " of novelty.
The course of work of the present invention: LED is paster, and what we needed novelty carries out the selection of special spectrum light source according to plant growth attribute.The photosynthesis of most of green plantss is the most responsive to 660nm.Meanwhile, we can be according to place, the outside natural conditions personalized designs illumination facilities implementing to produce.Can be simply to cover light screening material, but will reach best effect, or suggestion can be manufactured personalized illumination incubator.Finally, the illumination condition that also needs to consider Various Seasonal is adjusted light application time.
Taking the stem of noble dendrobium as examination material, the invention will be further described below, but be not limited to the stem of noble dendrobium, need to carry out photosynthetic plant and all can realize.
Choose 120 of the consistent and sturdy stem of Dendrobium sections of growth, be divided into 2 groups, 60 every group.Clip is about the branch section of 2cm, and base portion is whittled into lozenges, and by the naphthalene of 10PPM acid treatment 12 hours for base portion, then fast numerous intelligent seedbed in natural daylight and the ruddiness seedbed that has plant growth lamp light filling respectively, observes, records the growing state of stem section.Measuring chlorophyll content extraction, in the 3rd 6 12 days cultivating, evenly gets the equal position of each processing blade 0.2g, shred, acetone with 1:1: soaked in absolute ethyl alcohol, be placed in 40 degree insulating boxs and extract and after 24 hours, measure the wavelength 652nm OD of place value, calculate chlorophyll content.Soluble sugar is measured by 3,5 dinitrosalicylic acid methods, the active sulfanilamide (SN) colorimetric method for determining of using of nitrate reductase (NR). obtain following result:
1, cultivate that the stem section under ruddiness is compared with taking root early under natural daylight after 40 days, final radical is many, and rooting rate is up to 100%, and root is many and strengthen.Leaf look dark green, and stem is sturdy, and seedling growing way is vigorous.In whole incubation, under ruddiness, the growing way of material is obviously better than under natural daylight, shows that the artificial light of the present invention (ruddiness) has the effect (table 1) that promotes that the stem of noble dendrobium is taken root.
The stem of noble dendrobium branch section comparison of taking root under the artificial light of table 1 the present invention and natural light irradiation
Process Initial rootage duration/sky Radical/strain at most Mean elements Rooting rate % Have or not lateral root to occur
Natural daylight 11 18 14 89 Have
Ruddiness 8 35 29 100 Have
Note: in table, data are to observe the record data of 40 days, i.e. the stem of noble dendrobium branch section comparison of taking root under ruddiness and natural light irradiation.
2, in stem section process of growth, no matter be under natural daylight or under ruddiness, rear increasing all first falls in chlorophyll content.But chlorophyll content, higher than under natural daylight, illustrates that ruddiness shows obvious facilitation to chlorophyllous formation under ruddiness, and along with this result of increase more obvious (table 2) of cultivated days.Under ruddiness, plant strain growth gesture is better, may be that photosynthesis is more vigorous because plant body inner chlorophyll content is higher, has the synthetic of more carbohydrate, thereby provides due to sufficient matter and energy for the growth of plant.
Chlorophyll and soluble sugar content and nitrate reductase activity comparison under table 2 natural daylight and ruddiness
3, cultivate low than the 15th day of the soluble sugar content of the 9th day, and more than declining under natural daylight under ruddiness, the stem section under ruddiness is taken root also compared with the morning under natural daylight.After 15 days, the soluble sugar content under ruddiness is than high under natural daylight, this may with chlorophyll content under ruddiness higher (table 2), photosynthesis is more vigorous relevant.
4, under ruddiness, in stem section, NR activity is obviously greater than (table 2) under natural daylight.Visible red can promote stem of Dendrobium section nitrogen metabolism.

Claims (7)

1. an artificial light seedling cultivation method for Promoting plant growth and differentiation, is characterized in that at plant growth and in the idiophase, adopts LED SMD lamp as light source, and controlling and keeping optical wavelength is 610 ~ 660nm.
2. the artificial raise seedling method of Promoting plant growth according to claim 1 and differentiation, is characterized in that described plant is for need to carry out photosynthetic plant.
3. the artificial raise seedling method of Promoting plant growth according to claim 1 and 2 and differentiation, is characterized in that described plant is the one in the stem of noble dendrobium, cucumber, tomato, capsicum, potato, eggplant, konjaku, celery, asparagus, fresh kidney beans, onion, cabbage mustard, tulip, hyacinth, chrysanthemum, rose, Chinese rose.
4. the artificial raise seedling method of Promoting plant growth according to claim 1 and differentiation, is characterized in that the irradiation time of described light source is 14 ~ 18h.
5. the artificial raise seedling method of Promoting plant growth according to claim 4 and differentiation, is characterized in that described irradiation time is 16h.
6. according to the artificial raise seedling method of the Promoting plant growth described in claim 1 or 4 and differentiation, it is characterized in that described light source irradiation time is that the front 7 ~ 8h of sunset time is to post sunset 7 ~ 8h.
7. the artificial raise seedling method of Promoting plant growth according to claim 1 and differentiation, is characterized in that described optical wavelength is 660nm.
CN201410094091.XA 2014-03-14 2014-03-14 Artificial light seedling culture method capable of promoting plant growth and differentiation Pending CN103931429A (en)

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Cited By (10)

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CN104969863A (en) * 2015-07-08 2015-10-14 厦门加晟生物科技有限公司 Dendrobium officinale tissue culture propagation method
CN105145146A (en) * 2015-09-18 2015-12-16 湖南农业大学 Method for seedling and planting dendrobii officmalis caulis by adopting LED light source
CN105284446A (en) * 2015-09-29 2016-02-03 湖南绿米科技有限公司 Method utilizing LED plant growth light source to promote seedling nursing of solanaceous vegetables
CN105475009A (en) * 2016-01-25 2016-04-13 深圳市前海博森生物科技有限公司 Seedling cultivation method for strengthening growth vigor of plants by supplementing light
CN105660216A (en) * 2016-03-29 2016-06-15 衢州中恒农业科技有限公司 Technology for culturing hot pepper seedlings by applying LED plant lamps
CN105830685A (en) * 2016-03-21 2016-08-10 衢州中恒农业科技有限公司 Technology for growing cucumber seedlings by using LED plant lamps
CN105900638A (en) * 2016-04-21 2016-08-31 固镇县传红家庭农场 High-quality peanut planting method
CN106793424A (en) * 2017-02-13 2017-05-31 厦门迈信物联科技股份有限公司 One plant growth spectrum Automatic adjustment method
CN108184663A (en) * 2017-12-13 2018-06-22 上海市农业科学院 A kind of method that culture acquisition Chinese rose rooted seedling is handled by LED light
CN109122249A (en) * 2018-06-25 2019-01-04 华南农业大学 A kind of method that LED intermittent illumination cultivates cucumber seedling

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Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104969863A (en) * 2015-07-08 2015-10-14 厦门加晟生物科技有限公司 Dendrobium officinale tissue culture propagation method
CN105145146B (en) * 2015-09-18 2018-11-30 湖南农业大学 A method of dendrobium candidum nursery and plantation are carried out using LED light source
CN105145146A (en) * 2015-09-18 2015-12-16 湖南农业大学 Method for seedling and planting dendrobii officmalis caulis by adopting LED light source
CN105284446A (en) * 2015-09-29 2016-02-03 湖南绿米科技有限公司 Method utilizing LED plant growth light source to promote seedling nursing of solanaceous vegetables
CN105475009A (en) * 2016-01-25 2016-04-13 深圳市前海博森生物科技有限公司 Seedling cultivation method for strengthening growth vigor of plants by supplementing light
CN105830685A (en) * 2016-03-21 2016-08-10 衢州中恒农业科技有限公司 Technology for growing cucumber seedlings by using LED plant lamps
CN105830685B (en) * 2016-03-21 2018-12-11 衢州中恒农业科技有限公司 A method of cucumber seedling-raising is carried out with LED plant lamp
CN105660216A (en) * 2016-03-29 2016-06-15 衢州中恒农业科技有限公司 Technology for culturing hot pepper seedlings by applying LED plant lamps
CN105660216B (en) * 2016-03-29 2018-12-11 衢州中恒农业科技有限公司 A method of pepper seedling raising is carried out with LED plant lamp
CN105900638A (en) * 2016-04-21 2016-08-31 固镇县传红家庭农场 High-quality peanut planting method
CN106793424A (en) * 2017-02-13 2017-05-31 厦门迈信物联科技股份有限公司 One plant growth spectrum Automatic adjustment method
CN108184663A (en) * 2017-12-13 2018-06-22 上海市农业科学院 A kind of method that culture acquisition Chinese rose rooted seedling is handled by LED light
CN109122249A (en) * 2018-06-25 2019-01-04 华南农业大学 A kind of method that LED intermittent illumination cultivates cucumber seedling

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