CN108184663A - A kind of method that culture acquisition Chinese rose rooted seedling is handled by LED light - Google Patents
A kind of method that culture acquisition Chinese rose rooted seedling is handled by LED light Download PDFInfo
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- CN108184663A CN108184663A CN201711327687.XA CN201711327687A CN108184663A CN 108184663 A CN108184663 A CN 108184663A CN 201711327687 A CN201711327687 A CN 201711327687A CN 108184663 A CN108184663 A CN 108184663A
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- chinese rose
- seedling
- led light
- root
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Abstract
A kind of method that culture acquisition Chinese rose rooted seedling is handled by LED light, Chinese rose in vitro cuttings are inoculated in root media, first cultivated under daylight source irradiation, cultivate 5 7d, it is transferred to 15 20d of illumination cultivation under the compound light quality of LED light source again, obtains the test tube seedling taken root, transplants into matrix, hardening obtains Chinese rose rooted seedling.The present invention significantly improves the rooting rate in Chinese rose rooting process, and Root length growth is rapid, the Chinese rose rooted seedling rooting rate of acquisition is high by changing luminous environment condition, radical is more, and root long is long, and seedling quality is good, survival rate 100% after transplanting, and low energy consumption for culture rooting process, reduces production cost.
Description
Technical field
The invention belongs to Chinese rose technical field of tissue culture, and in particular to a kind of to handle culture acquisition Chinese rose by LED light
The method of rooted seedling.
Background technology
Chinese rose also known as catharanthus roseus, Chinese rose are rose family chamiso flowers, native to China, are ten big names of China
One of flower, because its flower hold beautiful, pattern is gorgeous, it is fragrant it is strong fragrant, the four seasons are normally opened, therefore have the laudatory title of " spending middle queen ".
At present, the reproduction technique of Chinese rose has still followed the methods of traditional cuttage, grafting, these methods limit Chinese rose
Breeding amount, it is impossible to meet the needs of the big production development of Chinese rose batch production.Therefore, further grinding to Chinese rose tissue culture technique
Study carefully and the marketization be the field technology development direction.
Light plays an important role to the growth and development of plant.With the development of photoelectric technology, LED (Light
EmittingDiode, light emitting diode) it succeeds in developing so that low energy consumption LED light source becomes a reality in the application of agriculture field.
LED not only has the advantages that small, long lifespan, low energy consumption, luminous efficiency is high, fever is low, the low photoelectric characteristic of fever, but also
Can the growth and development and photomorphogenesis of agro-ecology be promoted according to the accurate configuration for needing to carry out spectrum of agro-ecology, from
And improve its yield and quality.
Correlative study shows that different light medium has the growth and development of variety classes plant a different influences, exclusive excellent of LED
Point becomes one of important light source of Plant Tissue Breeding research.LED light source has been successfully applied to strawberry, lettuce, horse
In the tissue cultures of the various plants such as bell potato, pepper, cucumber, eucalyptus and Spathiphyllum kochii.In groups such as chrysanthemum, Lisianthus, purple dendrobiums
Training Miao Shangye has relevant report, but the proportioning combination of related LED different light mediums not yet appears in the newspapers to Chinese rose tissue-cultured seedling culture of rootage research
Road.
Invention content
The purpose of the present invention is to provide a kind of methods that culture acquisition Chinese rose rooted seedling is handled by LED light, pass through tune
Section root media proportioning, the light quality of LED light source combination and light quantity are cultivated with Chinese rose aseptic seedling is compared, the Chinese rose life of acquisition
Offspring rooting rate is high, and radical is more, and root long is long, and seedling quality is good, survival rate 100% after transplanting, and low energy consumption for culture rooting process,
Reference can be provided to be factory produced.
In order to achieve the above object, the present invention provides following technical solution：
A kind of method that culture acquisition Chinese rose rooted seedling is handled by LED light, includes the following steps：
1) it is inoculated with test tube seedling
The sterile single plant test tube seedling of Chinese rose of plant height >=2.0cm is taken, is inoculated in root media；
2) culture of rootage
The in vitro cuttings being inoculated in step 1) are first cultivated under daylight source, light application time 10-12h/d, temperature
22-27 DEG C, intensity of illumination 1000-2000lx, 5-7d is cultivated, then be transferred to illumination cultivation 15-20d under the compound light quality of LED light source,
Photosynthetically active radiation is 20~60 μm of olm-2·s-1, obtain the test tube seedling taken root；
3) hardening, transplanting
The test tube seedling taken root is taken out, agar is cleaned, transplants into matrix, under greenhouse experiment, shading, moisturizing, temperature 20-25
DEG C, after 10 days, removal shade, per a water is poured within 2-3 days, transplant planting after 20-40 days obtains Chinese rose rooted seedling.
Preferably, contain in the root media：1/4-1/2MS minimal mediums, methyl α-naphthyl acetate 0.05-0.5mg/L, sugarcane
Sugared 20-40g/L, agar 3-7g/L, pH 5.8-6.0.
Wherein, the compound light quality of the LED light source includes, and the feux rouges and wavelength that wavelength is 640-20nm is 450~
The blue light of 464nm.
Further, the light quantity proportioning in the compound light quality is feux rouges：Blue light=1：3-8.
Further, the transplanting medium is peat：Perlite=1-5：1, volume ratio.
Preferably, in step 3), the test tube seedling root long 1.0-1.5cm taken root.
Further, in step 3), 10/plant of Chinese rose rooted seedling radical ＞ of acquisition, root long ＞ 1.8cm.
Luminous environment has extreme influence to the growth and development of plant and morphogenesis, meanwhile, LED is applied in plant tissue culture
Light source can greatly reduce the energy consumption during extensive plant tissue culture, so as to reduce production cost.
LED belongs to cold light source, can be placed in and be immediated vicinity from plant without plant is burnt.LED integrated light-emitting plates
With good photoelectric characteristic, and its optical attenuation has regularity very much, carrys out convenience to the control strip of tissue culture light source, favorably
In the batch production and automated production of tissue culture.Secondly, LED light source has stronger transmittance than traditional fluorescent lamp light source,
Its effective radiation efficiency is also apparently higher than traditional fluorescent lamp.
The present invention is matched using the monochromatic spectrum that LED light source emits by white light, feux rouges, blue light and compound feux rouges-blue light
Combination carries out culture of rootage to Chinese rose, measures and after data statistic analysis, finally filter out the illumination that suitable Chinese rose takes root
Environment, with reference to the present invention culture medium, achieve the purpose that promoted seedling quality and reduce energy consumption, be LED light source in Chinese rose
Technical Reference is provided in propagation in scale.
The compound light quality of feux rouges-blue light in the present invention, the monochromatic red-light spectrum (615~650nm) of LED transmittings, blue light light
It composes (450~480nm), matches with the absorbing wavelength of photosynthetic pigments, especially chlorophyll a, b, absorbed with Photosynthetic Pigment
Spectrum coincide, and Chinese rose Aseptic Seedling Growth is healthy and strong, promotes the generation of Chinese rose root system, increases the quantity and length of root, and shortening is taken root
It is time, apparent to the rooting efficiency of Chinese rose aseptic seedling.
1/2-1/4MS minimal mediums of the present invention are the MS announced with reference to Murashige and Skoog in 1962
The concentration of a great number of elements, trace element, molysite and organic substance is reduced to the 1/2-1/4 of original content, matched by culture medium prescription
Concentration setting, the pH value requirement of the sucrose and agar of the present invention are closed, hormone is added, obtains root media, Chinese rose root growth
Comparatively fast, root system and seedling quality are preferable.
Compared with prior art, the present invention has the advantages that：
The present invention significantly improves the rooting rate in Chinese rose rooting process by changing luminous environment condition, and rooting rate is from common
60% under daylight source culture is increased to 100%, and radical increases to 10 or more/strain from 5.2/plant, and root long is from 0.86cm
Increase to 2.23cm, and plant height and leaf area are all suitable with common fluorescent lamp.
The present invention is taken root soon, and 7d or so can take root, and Root length growth is rapid, can shorten incubation time, root long
During 1.0-1.5cm, you can carry out acclimatization and transplants.
The present invention is using the compound light quality of LED light source as culture light source, and low energy consumption, so as to reduce production cost.
Description of the drawings
Fig. 1 is the Chinese rose rooted seedling in the embodiment of the present invention 1.
Specific embodiment
Below in conjunction with specific embodiment, the invention will be further described.
Embodiment 1
By the in vitro cuttings (plant height >=2.0cm) that early period, tissue culture obtained, one plant of strain is individually separated, is then vertically inoculated in
On root media, wherein, the root media is 1/4MS minimal medium+NAA 0.1mg/L+ sucrose 30g/L+ agar
4g/L, pH 5.8-6.0, condition of culture：25 DEG C of temperature, common daylight light irradiation, light application time 12h/d, intensity of illumination
1500lx cultivates 5d.
Then, the compound light quality illumination of LED light source, feux rouges (wavelength 640nm) are transferred to：Blue light (wavelength 460nm)=1：Under 3
20d is cultivated, photosynthetically active radiation is 20~30 μm of olm-2·s-1, 22-27 DEG C of cultivation temperature, light application time 10-12h/d,
Root long is obtained as 2cm or so, the rooted seedling of every plant of 13 roots.
The test tube seedling taken root in bottle is taken out, cleans the agar on root system, transplants in 32 hole disks, greenhouse item
Under part, shading, holding air and matrix moistening, 25 DEG C or so of temperature after 15 days, remove shading and carry out full exposure, every to pour within 2-3 days
Water, transplant planting after 1 month, survival rate 100%, referring to Fig. 1, it is seen then that the root system of Chinese rose rooted seedling is healthy and strong, growth of seedling
It is vigorous.
Embodiment 2
By the in vitro cuttings (plant height >=2.0cm) that early period, tissue culture obtained, one plant of strain is individually separated, is then vertically inoculated in
On root media, wherein, the root media is 1/3MS minimal medium+NAA 0.3mg/L+ sucrose 35g/L+ agar
5g/L, pH 5.8-6.0.Condition of culture, 25 DEG C of temperature, common daylight light irradiation, light application time 10h/d, intensity of illumination
2000lx cultivates 7d.
Then, the compound light quality illumination of LED light source, feux rouges (wavelength 650nm) are transferred to：Blue light (wavelength 450nm)=1：Under 5
15d is cultivated, photosynthetically active radiation is 50~60 μm of olm-2·s-1, 22-27 DEG C of cultivation temperature, light application time 10-12h/d,
Root long is obtained as 1.5cm or so, the rooted seedling of every plant of 13 roots.
The test tube seedling taken root in bottle is taken out, cleans the agar on root system, transplants in 32 hole disks.Greenhouse item
Under part, shading keeps air and matrix moistening, 25 DEG C or so of temperature.After 10 days, full exposure, per pouring within 2-3 days a water, 1 month
Transplant planting afterwards, survival rate 100%.
Embodiment and comparative example
In addition to LED illumination treatment conditions are different, remaining incubation step and condition are the same as embodiment 1.
Embodiment 3-5 and comparative example 1-3 is using the monochromatic spectrum that LED light source emits by white light, feux rouges, blue light and compound
The situation of taking root of the totally 6 kinds of proportioning combinations of feux rouges-blue light, specific light processing and Tissue Culture Shoot of Rosa Chinensis Jacq is referring to table 1.
Claims (7)
1. a kind of method that culture acquisition Chinese rose rooted seedling is handled by LED light, includes the following steps：
1) it is inoculated with test tube seedling
The sterile single plant test tube seedling of Chinese rose of plant height >=2.0cm is taken, is inoculated in root media；
2) culture of rootage
The in vitro cuttings being inoculated in step 1) are first cultivated under daylight source, light application time 10-12h/d, temperature 22-27
DEG C, intensity of illumination 1000-2000lx cultivates 5-7d, then is transferred to illumination cultivation 15-20d under the compound light quality of LED light source, photosynthetic
Net long wave radiation is 20~60 μm of olm-2·s-1, obtain the test tube seedling taken root；
3) hardening, transplanting
The test tube seedling taken root is taken out, agar is cleaned, transplants into matrix, under greenhouse experiment, shading, moisturizing, 20-25 DEG C of temperature,
After 10 days, removal shade, per a water is poured within 2-3 days, transplant planting after 20-40 days obtains Chinese rose rooted seedling.
2. the method for culture acquisition Chinese rose rooted seedling is handled by LED light according to claim 1, which is characterized in that described
Contain in root media：1/4-1/2MS minimal mediums, methyl α-naphthyl acetate 0.05-0.5mg/L, sucrose 20-40g/L, agar 3-
7g/L, pH 5.8-6.0.
3. the method for culture acquisition Chinese rose rooted seedling is handled by LED light according to claim 1, which is characterized in that described
The compound light quality of LED light source includes, the blue light that the feux rouges and wavelength that wavelength is 615-650nm are 450~480nm.
4. the method for culture acquisition Chinese rose rooted seedling is handled by LED light according to claim 1, which is characterized in that described
Light quantity proportioning in compound light quality is feux rouges：Blue light=1：3-8.
5. the method for culture acquisition Chinese rose rooted seedling is handled by LED light according to claim 1, which is characterized in that described
Transplanting medium is peat：Perlite=1-5：1, volume ratio.
6. the method for culture acquisition Chinese rose rooted seedling is handled by LED light according to claim 1, which is characterized in that step
3) in, the test tube seedling root long 1.0-1.5cm taken root.
7. the method for culture acquisition Chinese rose rooted seedling is handled by LED light according to claim 1, which is characterized in that step
3) in, 10/plant of Chinese rose rooted seedling radical ＞ of acquisition, root long ＞ 1.8cm.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711327687.XA CN108184663A (en) | 2017-12-13 | 2017-12-13 | A kind of method that culture acquisition Chinese rose rooted seedling is handled by LED light |
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| CN201711327687.XA CN108184663A (en) | 2017-12-13 | 2017-12-13 | A kind of method that culture acquisition Chinese rose rooted seedling is handled by LED light |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112438129A (en) * | 2020-11-18 | 2021-03-05 | 福建省中科生物股份有限公司 | Method for promoting industrial hemp rooting |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103931429A (en) * | 2014-03-14 | 2014-07-23 | 云南云琦丹生物科技有限公司 | Artificial light seedling culture method capable of promoting plant growth and differentiation |
| CN106613994A (en) * | 2017-01-19 | 2017-05-10 | 上海市农业科学院 | Method for acquiring fragrant plantain lily herb rooting seedlings by LED (light-emitting diode) light sources |
-
2017
- 2017-12-13 CN CN201711327687.XA patent/CN108184663A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103931429A (en) * | 2014-03-14 | 2014-07-23 | 云南云琦丹生物科技有限公司 | Artificial light seedling culture method capable of promoting plant growth and differentiation |
| CN106613994A (en) * | 2017-01-19 | 2017-05-10 | 上海市农业科学院 | Method for acquiring fragrant plantain lily herb rooting seedlings by LED (light-emitting diode) light sources |
Non-Patent Citations (2)
| Title |
|---|
| 吕永平等: "光照环境对大花月季组织培养的影响", 《浙江农业学报》 * |
| 李坤峰: "大花月季脱病毒苗产业化快繁体系建立研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112438129A (en) * | 2020-11-18 | 2021-03-05 | 福建省中科生物股份有限公司 | Method for promoting industrial hemp rooting |
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Application publication date: 20180622 |