CN112438129A - Method for promoting industrial hemp rooting - Google Patents
Method for promoting industrial hemp rooting Download PDFInfo
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- CN112438129A CN112438129A CN202011291875.3A CN202011291875A CN112438129A CN 112438129 A CN112438129 A CN 112438129A CN 202011291875 A CN202011291875 A CN 202011291875A CN 112438129 A CN112438129 A CN 112438129A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
- A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
Abstract
The invention discloses a method for promoting industrial hemp to root, which comprises the step of adopting blue light with the wavelength of 410-460 nm as an irradiation light source during indoor cutting propagation. The method of the invention can promote rooting, advance seedling emergence, shorten production period, and has stronger root growth potential, thus being beneficial to the later-stage cultivation of strong seedlings. In addition, the invention adopts the blue light with single wavelength for irradiation, is easy to operate and can reduce the production cost.
Description
Technical Field
The invention belongs to the technical field of industrial hemp planting, and particularly relates to a method for promoting industrial hemp rooting.
Background
The medical industrial hemp is a plant of the genus cannabis in the family of Moraceae, has high comprehensive utilization value, and is widely applied to the fields of fiber raw materials, functional food production, new materials and medical health care.
The propagation method of the medical industrial hemp mainly comprises seed propagation and cutting propagation. The cutting propagation can make the seedlings neat and consistent and fully maintain the characteristics of the original variety, so the cutting propagation is adopted more and more in recent years.
The traditional cuttage method is generally carried out outdoors by using natural light, and the cuttage of part of indoor cultivation mainly adopts a fluorescent lamp as a light source. The fluorescent lamp belongs to a full spectrum light source, and the light source is slow in rooting and long in seedling emergence time.
Disclosure of Invention
In view of the above, the present invention aims to provide a method for promoting industrial hemp rooting.
The invention adopts the specific technical scheme that:
a method for promoting industrial hemp rooting comprises adopting blue light with wavelength of 410-460 nm as irradiation light source in indoor cuttage propagation.
Preferably, the wavelength of the blue light is 410 nm.
Preferably, the environmental conditions during cutting propagation are as follows: the temperature is 25-27 ℃, the light intensity is 61-80 umol/square meter/s, and the relative humidity of air is 70-80%.
Preferably, in the cutting propagation, the cutting substrate is soaked in a complete nutrient solution with EC of 0.4-0.5ms/cm and PH of 5.6-6.0.
Preferably, the relative water content of the cutting medium is controlled between 75 and 85 percent during cutting propagation.
Preferably, the cutting substrate is rock wool.
Preferably, the formula of the total nutrient solution is as follows: fertilizer A (ammonium calcium nitrate 22.72g/L, KNO312g/L, EDTA-Fe 0.96g/L) and B fertilizer (KH)2PO4 11.738g/L,MgSO4·7H2O 10.138g/L，MgCl2·6H2O0.666 g/L) and C fertilizer (MnSO)4·H2O 113.2mg/L，H3BO3 50mg/L,ZnSO4·7H2O 38mg/L，CuSO4·5H2O 16.6mg/L，Na2MoO4 4.6mg/L,CoCl2·6H2O 8.6mg/L，NiCl2·6H2O8.6 mg/L) is prepared into concentrated mother liquor, and then diluted into working solution with EC of 0.4-0.5ms/cm and PH of 5.6-6.0.
Preferably, the daily maintenance of the cutting propagation is as follows: and immediately spraying a foliar fertilizer after the cuttage is finished, wherein the foliar fertilizer is a mixed solution of 0.1 percent of urea and 0.1 percent of monopotassium phosphate, spraying the foliar fertilizer twice every day, and dipping the rock wool blocks on the cuttage seedlings in the full nutrient solution once every 2-3 days.
Correspondingly, the invention also provides an indoor cutting propagation method for industrial cannabis sativa, which comprises the following steps:
(1) preparation work before cuttage: cleaning and sterilizing the seedling raising box for later use, soaking the cuttage matrix in a full nutrient solution with EC of 0.4-0.5ms/cm and PH of 5.6-6.0, controlling the relative water content of the cuttage matrix to be 75-85% by a weighing method, placing the cuttage matrix in the seedling raising box for later use, and arranging a blue light lamp with the wavelength of 410-460 nm in the seedling raising box.
(2) Selecting cutting shoots: selecting cutting slips from a strong stock plant without disease and insect pest infection, and pruning: the length is about 18cm, the thickness of the base part is 2.0-3.0mm, one terminal bud and two axillary buds are reserved, and the corresponding leaves of the axillary buds are reserved;
(3) preparing a rooting agent: diluting 5% indoliding-naphthylacetic acid by 200 times to prepare a rooting solution, soaking the base part of the trimmed cutting in the rooting solution for 1min, then inserting the processed cutting into rock wool blocks with the insertion depth of about 2.5cm, and turning on a blue light lamp;
(4) and (4) daily maintenance: and immediately spraying a foliar fertilizer after the cuttage is finished, wherein the foliar fertilizer is a mixed solution of 0.1 percent of urea and 0.1 percent of monopotassium phosphate, and then spraying the foliar fertilizer once every day in the morning and afternoon, and dipping the cuttage seedlings in the full nutrient solution once every 2-3 days.
Preferably, the cutting substrate is rock wool.
Preferably, the cutting propagation environmental conditions are as follows: the indoor temperature of the seedling raising room is 25-27 ℃, the light intensity is 61-80 umol/square meter/s, and the relative air humidity is 70-80%; the humidity in the seedling box is 80-90%.
Preferably, the cutting is cut from the stock immediately before its base is soaked in RO water.
The invention has the beneficial effects that: the method of the invention can promote rooting, advance seedling emergence, shorten production period, and has stronger root growth potential, thus being beneficial to the later-stage cultivation of strong seedlings. In addition, the invention adopts the blue light with single wavelength for irradiation, is easy to operate and can reduce the production cost.
Drawings
FIG. 1 is a graph of the rate of rooting of industrial hemp as a function of time for different lamp treatments of example 1;
FIG. 2 is a graph of the rate of rooting of industrial hemp as a function of time for different lamp treatments of example 2;
FIG. 3 is a graph of the rooting rate of industrial hemp as a function of time for different lamp treatments at room temperature of 21-23 ℃ in example 3;
FIG. 4 is a graph of the rooting rate of industrial hemp as a function of time for different lamp treatments at room temperature of 23-25 ℃ in example 3;
FIG. 5 is a graph of the rooting rate of industrial hemp as a function of time for the different lamp treatments at room temperature of 25-27 ℃ in example 3.
Detailed Description
The present invention will be described in further detail with reference to examples, but the embodiments of the present invention are not limited thereto, and various substitutions and alterations can be made without departing from the technical idea of the present invention as described above, according to the common technical knowledge and the conventional means in the field.
Example 1
And carrying out a cutting propagation test in an artificial light indoor environment at 8 month and 7 days in 2020. The lamp types used are T5 lamp tube (CK processing), blue light 410nm and blue light 450nm lamps respectively, and the specific test steps are as follows:
(1) and (5) preparing before cuttage. The three boxes of the seedling raising box are cleaned and sterilized for standby, agricultural rock wool blocks (with the specification of 3.6 x 4cm) are soaked in a full nutrient solution with the EC of 0.4-0.5ms/cm and the PH of 5.6-6.0, the relative water content of the rock wool is controlled to be 75-85% by a weighing method, the relative water content of the rock wool is kept between (the weight of the rock wool when the rock wool absorbs water-the weight of the rock wool)/(the weight of the rock wool when the rock wool fully absorbs water-the weight of the rock wool) is 100% }, the rock wool is placed in the seedling raising box for standby, 15 rock wool blocks in each box are used, namely 5 plants are planted by each lamp, and the operation is repeated for 3 times.
(2) And selecting the cutting slips. Selecting cutting slips from a strong stock plant which is not infected by diseases and insect pests, wherein the cut cutting slips require: the length is about 18cm, the thickness of the base part is 2.0-3.0mm, one terminal bud and two axillary buds are reserved, and the corresponding leaves of the axillary buds are reserved. The cuttings are cut from the stock and the base is immediately soaked in RO water.
(3) Preparing a rooting agent, diluting 5% of indin-naphthylacetic acid (Guoguang-trypan) by 200 times to prepare a rooting solution, soaking the trimmed base part of the cutting in the rooting solution for 1min, and then inserting the processed cutting into rock wool blocks with the insertion depth of about 2.5 cm.
(4) And (3) controlling environmental conditions: the indoor temperature of the seedling raising room is 23-25 ℃, the light intensity is 40-60 umol/square meter/s, and the relative air humidity is 70-80%; the humidity in the seedling raising box is preferably 80-90%.
(5) And (5) performing daily maintenance. And immediately spraying a foliar fertilizer after the cuttage is finished, wherein the foliar fertilizer is a mixed solution of 0.1 percent of urea and 0.1 percent of monopotassium phosphate, and then spraying the foliar fertilizer once in the morning and afternoon every day. Every 2-3 days, the rockwool blocks on the cutting seedlings need to be dipped with a complete nutrient solution (EC is 0.4-0.5ms/cm, PH is 5.6-6.0). And (5) carrying out the test till 8d, counting the rooting rate of each treatment, carrying out the test till 12d, counting the rooting rate, the rooting number and the average root length of each treatment, and comparing results shown in table 1 and figure 1.
TABLE 1 Effect of different Lamp treatments on rooting of Industrial hemp cuttings
Note: time 12d is counted.
As can be seen from FIG. 1 and Table 1, the rooting speed of the industrial hemp treated with the blue light 410mm of the example 1 is significantly faster than that of the control group (T5), the rooting time and the emergence time are both 1 day earlier than that of the control group, the average root length is 1.06cm longer than that of the control group, and the rooting number is 17.93 more than that of the control group. The rooting speed of the blue light treatment of 450mm is not obviously different from that of the T5 treatment, but the roots are longer and the rooting quantity is more.
Example 2
And carrying out a cutting propagation test in an artificial light indoor environment at 8 months and 8 days in 2020. The lamp types used are T5 lamp tube (CK processing), blue light 410nm, blue light 430nm, blue light 450nm and blue light 460nm lamps respectively, and the specific test steps are as follows:
(6) and (5) preparing before cuttage. The three boxes of the seedling raising box are cleaned and sterilized for standby, agricultural rock wool blocks (with the specification of 3.6 x 4cm) are soaked in a full nutrient solution with the EC of 0.4-0.5ms/cm and the PH of 5.6-6.0, the relative water content of the rock wool is controlled to be 75-85% by a weighing method, the relative water content of the rock wool is kept between (the weight of the rock wool when the rock wool absorbs water-the weight of the rock wool)/(the weight of the rock wool when the rock wool fully absorbs water-the weight of the rock wool) is 100% }, the rock wool is placed in the seedling raising box for standby, 15 rock wool blocks in each box are used, namely 5 plants are planted by each lamp, and the operation is repeated for 3 times.
(7) And selecting the cutting slips. Selecting cutting slips from a strong stock plant which is not infected by diseases and insect pests, wherein the cut cutting slips require: the length is about 18cm, the thickness of the base part is 2.0-3.0mm, one terminal bud and two axillary buds are reserved, and the corresponding leaves of the axillary buds are reserved. The cuttings are cut from the stock and the base is immediately soaked in RO water.
(8) Preparing a rooting agent, diluting 5% of indin-naphthylacetic acid (Guoguang-trypan) by 200 times to prepare a rooting solution, soaking the trimmed base part of the cutting in the rooting solution for 1min, and then inserting the processed cutting into rock wool blocks with the insertion depth of about 2.5 cm.
(9) And (3) controlling environmental conditions: the indoor temperature of the seedling raising room is 23-25 ℃, the light intensity is 40-60 umol/square meter/s, and the relative air humidity is 70-80%; the humidity in the seedling raising box is preferably 80-90%.
(10) And (5) performing daily maintenance. And immediately spraying a foliar fertilizer after the cuttage is finished, wherein the foliar fertilizer is a mixed solution of 0.1 percent of urea and 0.1 percent of monopotassium phosphate, and then spraying the foliar fertilizer once in the morning and afternoon every day. Every 2-3 days, the rockwool blocks on the cutting seedlings need to be dipped with a complete nutrient solution (EC is 0.4-0.5ms/cm, PH is 5.6-6.0). And (5) carrying out the test till 8d, counting the rooting rate of each treatment, carrying out the test till 12d, counting the rooting rate, the rooting number and the average root length of each treatment, and comparing results shown in a table 2 and a figure 2.
TABLE 2 Effect of different lamp treatments on rooting of cuttings of industrial hemp
Note: time 12d is counted.
As can be seen from FIG. 2 and Table 2, the rooting speed of each wavelength treatment using blue light of this example 2 is significantly faster than that of the control group (T5), the rooting starting time is 1 day earlier than that of the control group, and the emergence time is 1d earlier than that of the control group except for the treatment with blue light of 460 nm. The root length of the blue light treatment of 410nm, 430nm, 450nm and 460nm is respectively 2.09 times, 1.82 times, 2.15 times and 1.97 times of the T5 treatment, and the rooting number is respectively 2.02 times, 1.61 times, 1.96 times and 1.86 times of the T5 treatment. Therefore, the blue light treatment of each wavelength can promote the rooting of the hemp.
Example 3
And carrying out a cutting propagation test in an artificial light indoor environment at 9, 8 and 9 of 2020. The lamp types used are respectively T5 lamp tube (CK processing), blue light 410nm and blue light 450nm, the test is respectively carried out in laboratories with the room temperature of 21-23 ℃, 23-25 ℃ and 25-27 ℃, 3 lamp treatments are carried out in each room temperature treatment, 5 plants are treated in each room temperature treatment, the test is repeated for 3 times, and the specific test steps are as follows:
(11) and (5) preparing before cuttage. The three seedling raising boxes are cleaned and sterilized for later use, agricultural rock wool blocks (with the specification of 3.6 x 4cm) are soaked in a full nutrient solution with the EC of 0.4-0.5ms/cm and the PH of 5.6-6.0, the relative water content of rock wool is controlled to be 75-85% by a weighing method, the relative water content of rock wool is maintained to be (weight of rock wool when the rock wool absorbs water-weight of rock wool)/(weight of rock wool when the rock wool fully absorbs water-weight of rock wool) is 100%, and the rock wool is placed in the seedling raising boxes for later use.
(12) And selecting the cutting slips. Selecting cutting slips from a strong stock plant which is not infected by diseases and insect pests, wherein the cut cutting slips require: the length is about 18cm, the thickness of the base part is 2.0-3.0mm, one terminal bud and two axillary buds are reserved, and the corresponding leaves of the axillary buds are reserved. The cuttings are cut from the stock and the base is immediately soaked in RO water.
(13) Preparing a rooting agent, diluting 5% of indin-naphthylacetic acid (Guoguang-trypan) by 200 times to prepare a rooting solution, soaking the trimmed base part of the cutting in the rooting solution for 1min, and then inserting the processed cutting into rock wool blocks with the insertion depth of about 2.5 cm.
(14) And (3) controlling environmental conditions: the light intensity is 40-60 umol/square meter/s, and the relative air humidity is 70-80%; the humidity in the seedling raising box is preferably 80-90%.
(15) And (5) performing daily maintenance. And immediately spraying a foliar fertilizer after the cuttage is finished, wherein the foliar fertilizer is a mixed solution of 0.1 percent of urea and 0.1 percent of monopotassium phosphate, and then spraying the foliar fertilizer once in the morning and afternoon every day. Every 2-3 days, the rockwool blocks on the cutting seedlings need to be dipped with a complete nutrient solution (EC is 0.4-0.5ms/cm, PH is 5.6-6.0). And (4) carrying out the test till 6d, counting the rooting rate of each treatment, carrying out the test till 13d, counting the rooting rate, the rooting number and the average root length of each treatment, and comparing results shown in a table 3, a figure 4 and a figure 5.
TABLE 3 Effect of different lamps and different room temperature treatments on rooting of hemp cuttings
Note: time 13d is counted.
As can be seen from FIGS. 3, 4, 5 and 3, the rooting rates of the hemp cutting treated with blue light of 410nm and 450nm are significantly faster than those treated with T5 lamp at room temperature of 21-27 deg.C, and the emergence time of the hemp cutting is 1 day earlier than that treated with T5 lamp, wherein the emergence time is earliest when treated with 25-27 deg.C, then treated with 23-25 deg.C, and finally treated with 21-23 deg.C; the root length and the number of roots of the hemp cutting shoot under the blue light treatment are obviously higher than those of the hemp cutting shoot treated by T5 no matter what the room temperature treatment is. In summary, the blue light 410nm and the blue light 450nm treatments are better than the T5 treatments, and the room temperature is 25-27 ℃.
Although the embodiments have been described, once the basic inventive concept is obtained, other variations and modifications of these embodiments can be made by those skilled in the art, so that the above embodiments are only examples of the present invention, and not intended to limit the scope of the present invention, and all equivalent structures or equivalent processes using the contents of the present specification and drawings, or any other related technical fields, which are directly or indirectly applied thereto, are included in the scope of the present invention.
Claims (10)
1. A method for promoting industrial hemp to root is characterized by comprising the step of adopting blue light with the wavelength of 410-460 nm as an irradiation light source during indoor cutting propagation.
2. The method for promoting rooting in industrial hemp according to claim 1, wherein the wavelength of the blue light is 410 nm.
3. The method for promoting industrial hemp rooting according to claim 1 or 2, wherein the environmental conditions during cutting propagation are as follows: the temperature is 21-28 ℃, the light intensity is 30-90 umol/square meter/s, the relative air humidity is 60-80%, and the preferred is: the temperature is 25-27 ℃, the light intensity is 61-80 umol/square meter/s, and the relative humidity of air is 70-80%.
4. The method for promoting rooting in industrial cannabis sativa according to claim 1 or 2, wherein during cutting propagation, the cutting medium is soaked in a complete nutrient solution with an EC of 0.4-0.5ms/cm and a pH of 5.6-6.0.
5. The method of promoting rooting in industrial hemp according to claim 4, further comprising any one or more of the following features: (1) during cutting propagation, controlling the relative water content of a cutting medium to be 75-85%; (2) the cutting substrate is rock wool; (3) the formula of the total nutrient solution is as follows: fertilizer A (ammonium calcium nitrate 22.72g/L, KNO312g/L, EDTA-Fe 0.96g/L) and B fertilizer (KH)2PO411.738g/L,MgSO4·7H2O 10.138g/L，MgCl2·6H2O0.666 g/L) and C fertilizer (MnSO)4·H2O 113.2mg/L，H3BO3 50mg/L,ZnSO4·7H2O 38mg/L，CuSO4·5H2O 16.6mg/L，Na2MoO44.6mg/L,CoCl2·6H2O 8.6mg/L，NiCl2·6H2O8.6 mg/L) is prepared into concentrated mother liquor, and then diluted into working solution with EC of 0.4-0.5ms/cm and PH of 5.6-6.0.
6. The method for promoting rooting in industrial cannabis according to claim 5, wherein the daily maintenance of the cutting propagation is: and immediately spraying a foliar fertilizer after the cuttage is finished, wherein the foliar fertilizer is a mixed solution of 0.1 percent of urea and 0.1 percent of monopotassium phosphate, spraying the foliar fertilizer twice every day, and dipping the rock wool blocks on the cuttage seedlings in the full nutrient solution once every 2-3 days.
7. An indoor cutting propagation method for industrial cannabis sativa is characterized by comprising the following steps:
(1) preparation work before cuttage: cleaning and disinfecting the seedling raising box for later use, soaking the cuttage matrix in a full nutrient solution with EC of 0.4-0.5ms/cm and PH of 5.6-6.0, controlling the relative water content of the cuttage matrix to be 75-85% by a weighing method, placing the cuttage matrix in the seedling raising box for later use, and arranging a blue light lamp with the wavelength of 410-460 nm in the seedling raising box;
(2) selecting cutting shoots: selecting cutting slips from a strong stock plant without disease and insect pest infection, and pruning: the length is about 18cm, the thickness of the base part is 2.0-3.0mm, one terminal bud and two axillary buds are reserved, and the corresponding leaves of the axillary buds are reserved;
(3) preparing a rooting agent: diluting 5% indoliding-naphthylacetic acid by 200 times to prepare a rooting solution, soaking the base part of the trimmed cutting in the rooting solution for 1min, then inserting the processed cutting into rock wool blocks with the insertion depth of about 2.5cm, and turning on a blue light lamp;
(4) and (4) daily maintenance: and immediately spraying a foliar fertilizer after the cuttage is finished, wherein the foliar fertilizer is a mixed solution of 0.1 percent of urea and 0.1 percent of monopotassium phosphate, and then spraying the foliar fertilizer once every day in the morning and afternoon, and the rock wool blocks on the cuttage seedlings need to be dipped in the full nutrient solution once every 2-3 days.
8. The indoor cutting propagation method for industrial cannabis sativa according to claim 7, wherein the cutting medium is rock wool.
9. The indoor cutting propagation method for industrial cannabis sativa according to claim 7, wherein the cutting propagation environmental conditions are as follows: the indoor temperature of the seedling raising room is 25-27 ℃, the light intensity is 61-80 umol/square meter/s, and the relative air humidity is 70-80%; the humidity in the seedling box is 80-90%.
10. The indoor cutting propagation method for industrial cannabis sativa according to claim 7, wherein the base of the cutting shoot is soaked in RO water immediately after being cut from the stock plant.
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| PCT/CN2020/131641 WO2022104862A1 (en) | 2020-11-18 | 2020-11-26 | Method for promoting rooting of industrial hemp |
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| CN115024166B (en) * | 2022-07-08 | 2023-09-08 | 云南大学 | Method for cultivating full female seedlings of hermaphrodite industrial cannabis sativa |
| CN115152352B (en) * | 2022-07-25 | 2023-04-25 | 黑龙江省农业科学院大庆分院 | Industrial hemp aseptic seedling breeding method |
| CN115529952A (en) * | 2022-10-14 | 2022-12-30 | 江西省林业科学院 | Cuttage and rapid propagation method for Phoebe chekiangensis |
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| CN115989756A (en) * | 2022-12-13 | 2023-04-21 | 东北林业大学 | Cutting method of medicinal cannabis |
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| WO2022104862A1 (en) | 2022-05-27 |
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