CN112438195A - Industrial hemp water culture cuttage method - Google Patents
Industrial hemp water culture cuttage method Download PDFInfo
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- CN112438195A CN112438195A CN202011291869.8A CN202011291869A CN112438195A CN 112438195 A CN112438195 A CN 112438195A CN 202011291869 A CN202011291869 A CN 202011291869A CN 112438195 A CN112438195 A CN 112438195A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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Abstract
The invention discloses a water culture cutting method for industrial hemp, which controls the temperature of water culture cutting liquid to be 22-28 ℃. The method can promote rooting by controlling the liquid temperature, advance seedling emergence, shorten the production period, reduce the production cost, is simple and easy to operate, can make the root system grow more strongly by using the method, and is favorable for later-stage cultivation of strong seedlings. And the daily maintenance process can be simplified, and the maintenance efficiency is improved.
Description
Technical Field
The invention belongs to the technical field of industrial hemp planting, and particularly relates to a water culture cutting method for industrial hemp.
Background
The medicinal hemp is a plant of the cannabis genus in the Moraceae family, has high comprehensive utilization value, and is widely applied to the fields of fiber raw materials, functional food production, new materials and medical health care.
The propagation method of the medical industrial hemp mainly comprises seed propagation and cutting propagation. The cutting propagation can make the seedlings neat and consistent and fully maintain the characteristics of the original variety, so the cutting propagation is adopted more and more in recent years.
The traditional cuttage is generally matrix cuttage, and the cuttage for indoor cultivation mainly uses a fluorescent lamp as a light source and uses rock wool as a cultivation matrix. The cuttage seedling raising is carried out indoors, the temperature and the humidity of the environment can be accurately controlled, the cuttage seedling raising period is not affected theoretically, but the rooting is slower in winter and spring in the actual production, and the seedling raising time is long.
Disclosure of Invention
In view of the above, the present invention aims to provide an industrial marijuana water planting cutting method, which promotes rooting and seedlings emerge in advance by controlling the temperature of a water planting cutting solution.
The invention adopts the specific technical scheme that:
an industrial marijuana water culture cutting method is characterized in that the temperature of a water culture cutting solution is controlled to be 22-28 ℃.
Preferably, the temperature of the water culture cutting solution is 26 ℃.
Further, in the above hydroponic cutting method, the EC value of the hydroponic nutrient solution is in the range of 0.3-0.8ms/cm, the pH value is in the range of 5.5-6.5, and preferably: the EC value is in the range of 0.4-0.5ms/cm, and the pH value is in the range of 5.6-6.0.
Further, in the above hydroponic cutting method, the environmental conditions of the hydroponic cutting are as follows: the temperature is 21-28 ℃, the light intensity is 60-90 mu mol/square meter/s, and the preference is as follows: the temperature is 23-25 ℃, and the light intensity is 61-80 mu mol/square meter/s.
Further, in the above hydroponic cutting method, the daily management is as follows: spraying foliar fertilizer twice a day after cuttage is finished, wherein the foliar fertilizer is 0.1 percent of urea and 0.1 percent of KH2PO4Mixing the liquid, and dipping the rock wool blocks on the cutting seedlings with the full nutrient solution once every 2-3 days.
Correspondingly, the invention also provides an industrial hemp water culture cutting method, which comprises the following steps:
(1) preparing before cuttage: cleaning and sterilizing the seedling raising box for later use, soaking a cutting medium in a nutrient solution with EC of 0.3-0.8ms/cm and PH of 5.5-6.5, keeping the relative water content of the cutting medium at 75-85%, and placing the cutting medium in the seedling raising box for later use;
(2) preparing water culture cuttage: preparing a nutrient solution with EC of 0.3-0.8ms/cm and PH of 5.5-6.5, and setting the temperature of a water tank to be 22-28 ℃;
(3) selecting cutting shoots: selecting cutting slips from strong stock plants without diseases and insect pests infection: the length is 18cm, the base part thickness is 2.0-3.0mm, the terminal bud is one terminal bud and two axillary buds, the corresponding leaves of the axillary buds are reserved, and the base part of the cutting slip is immediately soaked in RO water after being cut off from the stock plant;
(4) a rooting agent: diluting 5% indoliding-naphthylacetic acid by 200 times to prepare a rooting solution, soaking the trimmed base part of the cutting slip in the rooting solution for 1min, then inserting the treated cutting slip into a cutting matrix with the insertion depth of 2-3cm, wherein the water culture insertion depth is that the base part of the cutting slip can be soaked in water by 1.5 cm;
(5) daily management: immediately spraying a leaf fertilizer after cuttage is finished, wherein the leaf fertilizer comprises 0.1 percent of urea and 0.1 percent of KH2PO4The mixed solution is sprayed once every day in the morning and afternoon, and the rockwool blocks on the cutting seedlings need to be dipped with the nutrient solution once every 2-3 days.
7. The industrial marijuana water culture cutting method according to claim 6, wherein the water culture cutting environmental conditions are as follows: the temperature is 21-28 ℃, the light intensity is 60-90 mu mol/square meter/s, and the preference is as follows: the temperature is 23-25 ℃, and the light intensity is 61-80 mu mol/square meter/s.
8. The industrial marijuana hydroponic cutting method according to claim 6, wherein the cutting medium is rock wool.
The invention has the beneficial effects that: the invention can promote rooting by controlling the temperature and/or the light intensity of the liquid, the seedling emergence is advanced, the production period is shortened, the production cost is reduced, the method is simple and easy to operate, and the method can ensure that the root growth vigor is stronger and is beneficial to the later-stage cultivation of strong seedlings. And the daily maintenance process can be simplified, and the maintenance efficiency is improved.
Drawings
FIG. 1 is a graph of the rooting rate of industrial hemp as a function of time for different liquid temperature treatments of example 1;
FIG. 2 is a graph of the rooting rate of industrial hemp as a function of time for different light intensity treatments of example 2.
Detailed Description
The present invention will be described in further detail with reference to examples, but the embodiments of the present invention are not limited thereto, and various substitutions and alterations can be made without departing from the technical idea of the present invention as described above, according to the common technical knowledge and the conventional means in the field. The water culture nutrient solutions used in the following examples 1-2 are all general nutrient solutions, and the formula is as follows: its nutrient components include nitrogen 6.08.5mmol/L, 4.0-6.0mmol/L potassium, 0.4-1.0mmol/L phosphorus, 0.8-1.8mmol/L calcium, 0.6-1.0mmol/L magnesium, 0.03-0.07mmol/L iron, 0.006-0.036mmol/L manganese, 0.0001-0.0003mmol/L copper, 0.018-0.05mmol/L boron, 0.007-0.03mmol/L zinc, 0.0002-0.0004mmol/L molybdenum, 0.7-1.0mmol/L sulfur, 0-0.007mmol/L chlorine, wherein the nitrogen-containing material comprises NH4+And NO3，NH4+-N/NO3The value of-N is 0.04-0.08.
Example 1
The cuttage propagation test is carried out in the artificial light indoor environment in 1 day 4 in 2020. The type of the used lamp is a T7 lamp tube, rock wool cuttage is used as a reference, different treatments are carried out in the research direction of liquid temperature 24 ℃, 26 ℃, 28 ℃ and 30 ℃, 9 plants are treated each, and the treatment is repeated for 3 times, wherein the specific test steps are as follows:
(1) and (5) rock wool cutting preparation. The three seedling raising boxes are cleaned and sterilized for later use, agricultural rock wool blocks (with the specification of 3.6 x 4cm) are soaked in a universal nutrient solution with the EC of 0.4-0.5ms/cm and the pH of 5.6-6.0, the relative water content of rock wool is controlled to be 75-85% by a weighing method, the relative water content of rock wool is maintained to be (weight of rock wool when the rock wool absorbs water-weight of rock wool)/(weight of rock wool when the rock wool fully absorbs water-weight of rock wool) is 100%, and the rock wool is placed in the seedling raising boxes for later use.
(2) And (5) preparing water culture cutting. The cultivation water circulation module set (Zhongke san an) is adopted, and the cultivation water circulation module set is cleaned and disinfected for standby. Preparing a universal nutrient solution with EC of 0.4-0.5ms/cm and pH of 5.6-6.0, placing a cypress-Kale electronic energy-saving heating rod (Shanghai pet crown) in each water tank, and setting the temperature at 24 deg.C, 26 deg.C, 28 deg.C and 30 deg.C respectively.
(3) And selecting the cutting slips. Selecting cutting slips from a strong stock plant which is not infected by diseases and insect pests, wherein the cut cutting slips require: the length is about 18cm, the thickness of the base part is 2.0-3.0mm, one terminal bud and two axillary buds are reserved, and the corresponding leaves of the axillary buds are reserved. The cuttings are cut from the stock and the base is immediately soaked in RO water.
(4) Preparing a rooting agent, diluting 5% of indagan-naphthylacetic acid (Guoshian-trypan) by 200 times to prepare a rooting solution, soaking the trimmed base part of the cutting in the rooting solution for 1min, then respectively inserting the treated cutting into a rock wool block and a cultivation groove, wherein the insertion depth of the rock wool is about 2.5cm, and the insertion depth of the water cultivation is preferably that the base part of the cutting can be soaked in water by 1.5 cm.
(5) And (3) controlling environmental conditions: the indoor temperature of the seedling raising room is 23-25 ℃, the light intensity is 40-60 mu mol/square meter/s, and the relative air humidity is 50-80%; the humidity in the seedling raising box is preferably 80-90%.
(6) And (5) performing daily maintenance. Immediately spraying a foliar fertilizer after the cuttage is finished, wherein the foliar fertilizer is prepared from 0.1 percent of urea and 0.1 percent of KH2PO4Mixing the solution, and spraying once every day in the morning and afternoon. Every 2-3 days, the rockwool blocks on the cutting seedlings need to be dipped with general nutrient solution (EC is 0.4-0.5ms/cm, pH is 5.6-6.0). And (4) carrying out the test till 5d, counting the rooting rate of each treatment, carrying out the test till 9d, counting the rooting rate, the basal rot rate, the rooting quantity and the average root length of the cuttings treated at different liquid temperatures, carrying out the cutting till 12d, counting the rooting rate, the basal rot rate, the rooting quantity and the average root length of the cuttings obtained by cutting with rock wool, and showing a comparison result in a chart 1 and a table 1.
TABLE 1 Effect of different liquid temperature treatments on rooting of cuttings of industrial hemp
Remarking: and (5) counting the CK rooting rate, the basal rot rate, the average root length and the rooting number for 12d of cuttage, and counting the rest treatment for 9 d.
The rooting speed of the water culture cutting treatment for improving the liquid temperature is obviously higher than that of rock wool Cutting (CK), the rooting starting time is 2-3d earlier than that of CK, the seedling emergence time is about 3d earlier than that of CK treatment, the treatment at the liquid temperature of 26 ℃ is optimal, the average root length and the rooting quantity are obviously different from that of CK treatment, and the root length and the rooting quantity are respectively 2.22 times and 1.69 times of that of CK treatment under the condition that the statistical time is 3d earlier than that of CK treatment.
Example 2
And carrying out a water culture cutting propagation test in an artificial light indoor environment at 8 month and 27 days in 2020. The lamp type is a T7 lamp tube, the liquid temperature is 26 ℃, 3 light intensity treatments are set, the light intensity is respectively 21-40 mu mol/square meter/s, 41-60 mu mol/square meter/s, 61-80 mu mol/square meter/s, 16 plants are treated for each treatment, and the test is repeated for 3 times, and the specific test steps are as follows:
(7) and (5) rock wool cutting preparation. The three seedling raising boxes are cleaned and sterilized for later use, agricultural rock wool blocks (with the specification of 3.6 x 4cm) are soaked in a universal nutrient solution with the EC of 0.4-0.5ms/cm and the pH of 5.6-6.0, the relative water content of rock wool is controlled to be 75-85% by a weighing method, the relative water content of rock wool is maintained to be (weight of rock wool when the rock wool absorbs water-weight of rock wool)/(weight of rock wool when the rock wool fully absorbs water-weight of rock wool) is 100%, and the rock wool is placed in the seedling raising boxes for later use.
(8) And (5) preparing water culture cutting. The cultivation water circulation module set (Zhongke san an) is adopted, and the cultivation water circulation module set is cleaned and disinfected for standby. Preparing a universal nutrient solution with EC of 0.4-0.5ms/cm and pH of 5.6-6.0, placing a cypress-Kale electronic energy-saving heating rod (Shanghai pet crown) in each water tank, and setting the liquid temperature at 26 ℃.
(9) And selecting the cutting slips. Selecting cutting slips from a strong stock plant which is not infected by diseases and insect pests, wherein the cut cutting slips require: the length is about 18cm, the thickness of the base part is 2.0-3.0mm, one terminal bud and two axillary buds are reserved, and the corresponding leaves of the axillary buds are reserved. The cuttings are cut from the stock and the base is immediately soaked in RO water.
(10) Preparing a rooting agent, diluting 5% of indagan-naphthylacetic acid (Guoshian-trypan) by 200 times to prepare a rooting solution, soaking the trimmed base part of the cutting in the rooting solution for 1min, then respectively inserting the treated cutting into a rock wool block and a cultivation groove, wherein the insertion depth of the rock wool is about 2.5cm, and the insertion depth of the water cultivation is preferably that the base part of the cutting can be soaked in water by 1.5 cm.
(11) And (3) controlling environmental conditions: indoor temperature of the seedling raising room is 23-25 ℃, and relative air humidity is 50-80%; the humidity in the seedling raising box is preferably 80-90%.
(12) And (5) performing daily maintenance. Immediately spraying a foliar fertilizer after the cuttage is finished, wherein the foliar fertilizer is prepared from 0.1 percent of urea and 0.1 percent of KH2PO4Mixing the solution, and spraying once every day in the morning and afternoon. Every 2-3 days, the rockwool blocks on the cutting seedlings need to be dipped with general nutrient solution (EC is 0.4-0.5ms/cm, pH is 5.6-6.0). The test is carried out to the 5 th day, the rooting rate of each treatment is counted, the test is carried out to the 9 th day, the rooting rate, the basal rot rate, the rooting quantity and the average root length of the cutting slips treated by different liquid temperatures are counted, and the results are comparedSee fig. 2, table 2.
TABLE 2 Effect of different light intensity treatments on rooting of cuttings of industrial hemp
Note: the rooting rate, the basal decay rate, the average root length and the rooting number counting time are all in 9 d.
As can be seen from the figure 2 and the table 2, the light intensity is 41-60 mu mol/square meter/s, the treatment of 61-80 mu mol/square meter/s is 1d earlier rooting and 1d early seedling emergence compared with the light intensity of 21-40 mu mol/square meter/s, and when seedlings emerge, the light intensity is 41-60 mu mol/square meter/s, the root length processed by 61-80 mu mol/square meter/s is respectively 1.97 times and 2.83 times of the light intensity 21-40 mu mol/square meter/s, the rooting quantity is respectively 1.47 times and 1.56 times of the light intensity 21-40 mu mol/square meter/s, the root length processed by 61-80 mu mol/square meter/s and the rooting quantity are respectively 1.44 times and 1.06 times of the light intensity 41-60 mu mol/square meter/s, therefore, the light intensity of 61-80 mu mol/square meter/s is most beneficial to rooting of the cutting shoots.
Although the embodiments have been described, once the basic inventive concept is obtained, other variations and modifications of these embodiments can be made by those skilled in the art, so that the above embodiments are only examples of the present invention, and not intended to limit the scope of the present invention, and all equivalent structures or equivalent processes using the contents of the present specification and drawings, or directly or indirectly using other related technical fields, are included in the scope of the present invention.
Claims (8)
1. An industrial marijuana water culture cutting method is characterized in that the temperature of water culture cutting liquid is controlled to be 22-28 ℃.
2. The industrial marijuana hydroponic cutting method according to claim 1, wherein the temperature of the hydroponic cutting liquid is 26 ℃.
3. An industrial marijuana hydroponic cutting method according to claim 1 or 2, characterized in that the EC value of the hydroponic nutrient solution is in the range of 0.3-0.8ms/cm, the pH value is in the range of 5.5-6.5, preferably: the EC value is in the range of 0.4-0.5ms/cm, and the pH value is in the range of 5.6-6.0.
4. The industrial marijuana hydroponic cutting method according to claim 1 or 2, characterized in that the environmental conditions of the hydroponic cutting are as follows: the temperature is 21-28 ℃, the light intensity is 60-90 mu mol/square meter/s, and the preference is as follows: the temperature is 23-25 ℃, and the light intensity is 61-80 mu mol/square meter/s.
5. The industrial cannabis hydroponic cutting method according to any one of claims 1 or 2, characterized in that the daily management of hydroponic cutting is as follows: spraying foliar fertilizer twice a day after cuttage is finished, wherein the foliar fertilizer is 0.1 percent of urea and 0.1 percent of KH2PO4Mixing the liquid, and dipping the rock wool blocks on the cutting seedlings with the full nutrient solution once every 2-3 days.
6. An industrial marijuana water culture cutting method is characterized by comprising the following steps:
(1) preparing before cuttage: cleaning and sterilizing the seedling raising box for later use, soaking a cutting medium in a nutrient solution with EC of 0.3-0.8ms/cm and PH of 5.5-6.5, keeping the relative water content of the cutting medium at 75-85%, and placing the cutting medium in the seedling raising box for later use;
(2) preparing water culture cuttage: preparing a nutrient solution with EC of 0.3-0.8ms/cm and PH of 5.5-6.5, and setting the temperature of a water tank to be 22-28 ℃;
(3) selecting cutting shoots: selecting cutting slips from strong stock plants without diseases and insect pests infection: the length is 18cm, the base part thickness is 2.0-3.0mm, the terminal bud is one terminal bud and two axillary buds, the corresponding leaves of the axillary buds are reserved, and the base part of the cutting slip is immediately soaked in RO water after being cut off from the stock plant;
(4) a rooting agent: diluting 5% indoliding-naphthylacetic acid by 200 times to prepare a rooting solution, soaking the trimmed base part of the cutting slip in the rooting solution for 1min, then inserting the treated cutting slip into a cutting matrix with the insertion depth of 2-3cm, wherein the water culture insertion depth is that the base part of the cutting slip can be soaked in water by 1.5 cm;
(5) daily management: immediately spraying foliar fertilizer after cuttage is finishedThe foliar fertilizer is 0.1 percent of urea and 0.1 percent of KH2PO4The mixed solution is sprayed once every day in the morning and afternoon, and the rockwool blocks on the cutting seedlings need to be dipped with the nutrient solution once every 2-3 days.
7. The industrial marijuana water culture cutting method according to claim 6, wherein the water culture cutting environmental conditions are as follows: the temperature is 21-28 ℃, the light intensity is 60-90 mu mol/square meter/s, and the preference is as follows: the temperature is 23-25 ℃, and the light intensity is 61-80 mu mol/square meter/s.
8. The industrial marijuana hydroponic cutting method according to claim 6, wherein the cutting medium is rock wool.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115989756A (en) * | 2022-12-13 | 2023-04-21 | 东北林业大学 | Cutting method of medicinal cannabis |
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| CN102919044A (en) * | 2012-11-22 | 2013-02-13 | 云南大学 | Cutting propagation method of industrial cannabis sativa |
| CN106900505A (en) * | 2017-03-03 | 2017-06-30 | 福建省中科生物股份有限公司 | A kind of artificial light environment cultural method of hemp |
| CN109452154A (en) * | 2018-11-09 | 2019-03-12 | 福建省中科生物股份有限公司 | Cuttage and seedling culture method in hemp room |
| CN109644721A (en) * | 2019-02-15 | 2019-04-19 | 福建省中科生物股份有限公司 | A kind of light source of indoor growing plant |
| CN110178558A (en) * | 2019-06-28 | 2019-08-30 | 广西壮族自治区林业科学研究院 | A kind of method of sweetgum Water culture nursery |
| CN110972918A (en) * | 2019-12-31 | 2020-04-10 | 云南省农业科学院经济作物研究所 | Water culture rooting method for cutting seedlings of medicinal industrial hemp |
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- 2020-11-18 CN CN202011291869.8A patent/CN112438195A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102919044A (en) * | 2012-11-22 | 2013-02-13 | 云南大学 | Cutting propagation method of industrial cannabis sativa |
| CN106900505A (en) * | 2017-03-03 | 2017-06-30 | 福建省中科生物股份有限公司 | A kind of artificial light environment cultural method of hemp |
| CN109452154A (en) * | 2018-11-09 | 2019-03-12 | 福建省中科生物股份有限公司 | Cuttage and seedling culture method in hemp room |
| CN109644721A (en) * | 2019-02-15 | 2019-04-19 | 福建省中科生物股份有限公司 | A kind of light source of indoor growing plant |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115989756A (en) * | 2022-12-13 | 2023-04-21 | 东北林业大学 | Cutting method of medicinal cannabis |
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