CN110972918A - Water culture rooting method for cutting seedlings of medicinal industrial hemp - Google Patents
Water culture rooting method for cutting seedlings of medicinal industrial hemp Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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Abstract
The invention discloses a method for water culture rooting of cutting seedlings of cannabis sativa in pharmaceutical industry. The method is characterized in that a special water culture device comprising an upper water tank, a lower water tank, a submersible pump and a foam plate which are nested is adopted, tap water, sterilized water or distilled water is used as water culture liquid, and the submersible pump is required to be in a working state in the cutting rooting process; before the flowering of the medical industrial hemp, the leaf spike at the top of a semi-lignified or lignified main branch or lateral branch is used as a cutting, the cutting is inserted into a planting hole on a foam board, and the lower part of the cutting, 2 cm-2.5 cm, is immersed into a water culture solution; water culture environmental conditions: the temperature is 25-35 ℃, the water temperature is 20-25 ℃, the illumination is natural light, and the cuttage seedlings are irradiated by natural astigmatism in the daytime; the water culture solution is sprayed on the leaf surface 4-6 times every day to keep the leaf surface humidity, the invention does not use chemical agents, the root hair of the cutting seedling can grow to more than 10cm in 12-22 days, the rooting rate of the cutting reaches 100%, the water culture solution does not need to be replaced in the midway, the cost is low, and the environment is protected.
Description
Technical Field
The invention belongs to the technical field of cuttage breeding of industrial hemp, and relates to a water culture rooting method for high-quality medicinal industrial hemp cuttage seedlings.
Background
The cannabis sativa L.in the pharmaceutical industry is an agricultural variety artificially bred from an annual herb cannabis sativa L.of the cannabis genus of the cannabinaceae family, and the hallucinogenic addiction component Tetrahydrocannabinol (THC) content in the plant is less than 0.3% (on dry matter), which cannot be directly utilized as a drug, but contains a plurality of cannabinol components such as Cannabidiol (CBD), cannabidiolic acid (CBDA), tetrahydrocannabinolic acid (THCA), Cannabinol (CBN), sub-Cannabidiol (CBDV) and the like. Clinical researches find that the components have the effects of resisting epilepsy, protecting the nervous system and the like. In particular, CBD has been marketed in a number of countries such as the United kingdom and Canada for 2 commercial drugs containing it as a main component, each of which is owned by GW pharmaceutical company in the United kingdom
Medicine for treating hepatitis
Can be used for treating intractable epilepsia diseases such as infantile severe myoclonic epilepsy, and adult multiple sclerosis.
Cultivation of cannabis in the pharmaceutical industry is one of the major problems facing the development of the industrial cannabis industry. The existing medicinal industrial hemp varieties are mostly male and female heteroplants, and are mostly in a direct seed sowing or seed seedling transplanting mode in production, so that the problem of individual difference of seed propagation cannot be fundamentally solved, and the cutting seedling culture is a vegetative propagation technology, and is one of the methods for quickly obtaining high-quality, tidy and consistent medicinal industrial hemp seedlings.
The existing industrial hemp cutting rooting methods comprise two methods, namely a cutting method adopting soil or rock wool absorbing nutrient solution as a matrix and a method adopting nutrient solution for culture. These methods have the following drawbacks: the soil is used as a substrate, so that the survival rate of the seedling is low; the cuttage method using rock wool absorbing nutrient solution as a substrate uses rooting powder, artificially prepared nutrient solution, phytohormone and other chemical reagents, and is not environment-friendly. For mass rooting propagation, the method for rooting culture by using the nutrient solution has the advantages of large using amount of the nutrient solution, low utilization rate, easy environmental pollution after wastewater discharge and high cost investment.
Disclosure of Invention
In order to solve the technical problems of high cost and environmental pollution of the existing nutrient solution cutting seedling rooting culture method, the invention provides a water culture rooting method for cutting seedlings of cannabis sativa in pharmaceutical industry.
The invention provides a water culture rooting method for cutting seedlings of Cannabis sativa in pharmaceutical industry, which comprises the following steps:
a, cultivating medicinal industrial hemp cutting seedlings by using a special water culture device to root, respectively containing water culture solution in an upper water tank and a lower water tank of the special water culture device, inserting cutting slips into planting holes of a foam board floating on the water surface in the upper water tank, immersing the lower parts of the cutting slips into the water culture solution by 2-2.5 cm, selecting 8-15 cm long main branch top leaf spikes with semi-lignified or lignified stems before the medicinal industrial hemp blooms, or 8-15 cm long side branch top leaf spikes with semi-lignified or lignified stems, and trimming the main branch top leaf spikes or the side branch top leaf spikes, wherein only spike tips and 1-2 leaves are reserved for the main branch top leaf spikes or the side branch top leaf spikes, and half leaves are cut off for each leaf; the water culture solution is tap water, sterilized water or distilled water;
step B, the water culture environmental conditions are as follows: the temperature is 25-35 ℃, the water temperature is 20-25 ℃, the illumination is natural light, and the cuttage seedlings are irradiated by natural astigmatism in the daytime;
c, the submersible pump is in an open working state in the whole water planting and rooting process of the cutting seedlings, so that the water planting liquid in the lower water tank is continuously pumped into the upper water tank through the submersible pump, and the water planting liquid is sprayed to the leaf surfaces of the cutting seedlings 4-6 times every day;
the special water culture device has the structure that: the device comprises an upper layer of water tank, a lower layer of water tank, a submerged pump and a foam board which are nested, wherein the lower layer of water tank and the upper layer of water tank are nested in the lower layer of water tank from bottom to top; a foam plate is arranged in the upper layer water tank, and an overflow hole is arranged on the upper tank wall of the upper layer water tank; water pumping holes with the interval of 20-30cm are formed in the bottom of the upper-layer water tank, a submersible pump is placed in the lower-layer water tank, a water outlet pipe of the submersible pump penetrates through the water pumping holes of the upper-layer water tank and part of the water outlet pipe penetrates through the tank body of the upper-layer water tank, and the contact part of the pipe wall of the water outlet pipe of the submersible pump and the water pumping holes is hermetically connected through a; planting holes with the interval of 4-6cm are formed in the foam board; the nesting is that a clamping groove is arranged at the edge of the top of the upper-layer water tank, the top of the lower-layer water tank is inserted into the clamping groove, the tank body of the upper-layer water tank is arranged in the inner cavity of the lower-layer water tank, and the bottom of the upper-layer water tank is not contacted with the submersible pump in the lower-layer water tank.
In the process of water culture rooting, the foam plate is positioned below the overflow hole or is flush with the overflow hole and is positioned above the submersible pump water outlet pipe in the upper-layer water tank.
Particularly, the diameter of a water pumping hole at the bottom surface of an upper layer water tank in the special water culture device is 1.8-2.2 cm; the aperture of the planting holes on the foam board is 2.0-2.5 cm.
Particularly, the depth of the upper layer water tank is 7-10 cm, and the thickness of the foam plate is 1.0-3.0 cm.
Compared with the prior art, the invention has the beneficial effects that:
1. the method does not use chemical agents, solves the technical problems that in the prior industrial production, a large amount of nutrient solution is adopted to cultivate the root seedlings, the cost of the required nutrient solution is high, and the discharge of waste liquid causes certain pollution to the environment.
2. The rooting rate is high. Compared with the conventional water culture method (contrast), the method greatly improves the rooting rate, and the rooting rate of the cutting seedlings reaches 100 percent.
3. The rooting time of the cutting seedlings is short, and the roots of the cutting seedlings can grow to be more than 10cm only in 12-22 days.
4. The hydroponic liquid does not need to be replaced in the hydroponic process, and the management is simple.
Drawings
FIG. 1: the structure of the special water culture device is shown schematically.
FIG. 2: schematic diagram of a foam board structure. A cutting is inserted into a planting hole on the foam board.
FIG. 3: upper water tank structure schematic diagram, wherein, left picture: a front view of the upper water tank; right panel: side view of the upper water tank.
FIG. 4: the structure of the lower water tank and the submersible pump is shown schematically.
Each of fig. 1-4 is labeled as: 1-foam plate, 2-upper layer water tank, 3-lower layer water tank, 4-submersible pump, 5-water pumping hole, 6-submersible pump water outlet pipe, 7-overflow hole, 8-planting hole, 9-clamping groove, 10-sealing ring, and 11-groove body of upper layer water tank.
Detailed Description
The present invention will be described in further detail with reference to specific examples, but conventional methods are not specifically described below.
Example 1
A, cultivating medicinal industrial hemp cutting seedlings by adopting a special water culture device to take roots, respectively containing water culture solution in an upper water tank 2 and a lower water tank 3 of the special water culture device, inserting cutting slips into planting holes 8 of a foam plate 1 floating on the water surface in the upper water tank 2, immersing the lower parts of the cutting slips into the water culture solution by 2-2.5 cm, selecting 8-15 cm long main branch top leaf spikes with semi-lignified or lignified stems before the medicinal industrial hemp blooms, or 8-15 cm long side branch top leaf spikes with semi-lignified or lignified stems, and trimming the main branch top leaf spikes or the side branch top leaf spikes, wherein only spike tips and 1-2 leaves are reserved for the main branch top leaf spikes or the side branch top leaf spikes, and half leaves are cut off for each leaf; the water culture solution is tap water, sterilized water or distilled water;
step B, the water culture environmental conditions are as follows: the temperature is 25-35 ℃, the water temperature is 20-25 ℃, the illumination is natural light, and the cuttage seedlings are irradiated by natural astigmatism in the daytime;
and step C, the submersible pump 4 is in an open working state in the whole water planting rooting process of the cutting seedlings, so that the water planting liquid in the lower-layer water tank 3 is continuously pumped into the upper-layer water tank 2 through the submersible pump 4, and 4-6 times of water planting liquid is sprayed to the leaf surfaces every day.
The special water culture device has the structure that: the device comprises an upper layer of water tank, a lower layer of water tank 3 and an upper layer of water tank 2 which is nested in the lower layer of water tank 3 from bottom to top, wherein the upper layer of water tank, the submersible pump and the foam board are nested in the upper layer of water tank; the foam board 1 is arranged in the upper layer water tank 2, and the upper tank wall of the upper layer water tank 2 is provided with an overflow hole 7; water pumping holes 5 with the interval of 20-30cm are formed in the bottom of the upper-layer water tank 2, a submersible pump 4 is placed in the lower-layer water tank 3, a water outlet pipe 6 of the submersible pump penetrates through the water pumping holes 5 of the upper-layer water tank and part of the water outlet pipe penetrates through a tank body 11 of the upper-layer water tank, and the pipe wall of the water outlet pipe 6 of the submersible pump is in sealing connection with the contact parts of the water pumping holes 5 through a; planting holes 8 with the interval of 4-6cm are formed in the foam board; the nesting is that a clamping groove 9 is arranged at the top edge of the upper-layer water tank 2, the top of the lower-layer water tank 3 is inserted into the clamping groove 9, the tank body 11 of the upper-layer water tank is arranged in the inner cavity of the lower-layer water tank 3, and the bottom of the upper-layer water tank 2 is not contacted with the submersible pump 4 in the lower-layer water tank 3. In the process of water culture rooting, the foam plate 1 is positioned below the overflow hole 7 or is flush with the overflow hole 7, and the foam plate 1 is positioned above the submersible pump water outlet pipe 6 in the upper-layer water tank 2.
And C, under the same water culture environment condition in the step B, observing parameters of the water culture device by using the same cutting seedlings, and carrying out the influence of water culture rooting of the cutting seedlings of the Cannabis sativa in the pharmaceutical industry by using tap water, wherein the influence is shown in table 1.
TABLE 1 influence of different parameter settings of special hydroponics device on rooting of medicinal industrial hemp cutting seedlings
The data in table 1 show that:
1. under the whole in-process immersible pump open mode of water planting, all promote the running water in the lower floor's basin to the upper water tank in through the outlet pipe, the smaller is the water lifting hole interval of upper water tank bottom, and the interval of cystosepiment planting hole is bigger, is favorable to the medicinal industry marijuana cutting to take root more, and the length of root is longer promptly, and the growth time of root is shorter more, at immersible pump water lifting hole interval 20-30cm, cystosepiment planting hole interval is 4-6cm within range, and 100% rooting rate can all be guaranteed.
2. Under the condition that the distance between the water-raising holes is too large and exceeds 30cm (contrast 1-2), even if the distance between the foam board planting holes is increased, 100 percent of rooting rate cannot be ensured.
3. The submersible pump is turned on until the water culture solution overflows and then is turned off, and the cutting seedlings of the hemp in the pharmaceutical industry can not root. The optimal parameters for hydroponic device preparation are therefore: the submersible pump is in an open state, the distance between the water pumping holes is 20cm, and the distance between the planting holes is 6 cm.
4. In the case of conventional tap water hydroponics where no submersible pump is used to extract water from the lower water tank to the upper water tank, the cutting seedlings of Cannabis sativa in the pharmaceutical industry could not root (control 5).
Example 2
Under the same water culture environment conditions described in the step B of the embodiment 1, the submersible pump is used to be in an open state, the spacing between the water-raising holes is 20cm, and the spacing between the planting holes is 6cm, and the influence of the selection of the water culture solution on the rooting of the cutting seedlings of the Cannabis sativa in the pharmaceutical industry is examined by using the same cutting seedlings, which is specifically shown in the table 2.
TABLE 2 influence of different hydroponic liquids of hydroponic devices with optimal parameters on rooting of cutting seedlings of medicinal industrial cannabis sativa
The data in table 2 show that: under the optimal parameter condition of the special water culture device, tap water, sterilized water or distilled water has no obvious difference.
Example 3: the special water culture device using the optimal parameters uses tap water as water culture solution, 4-6 times of water culture solution is sprayed on the leaf surface every day at intervals, the interval time is 1-2 hours every time, and the influence of the air temperature and the water temperature on the rooting of the cutting seedlings of the Cannabis sativa in the pharmaceutical industry is inspected, which is specifically shown in table 3.
TABLE 3 influence of air and water temperature on rooting of cutting seedlings of Cannabis sativa in pharmaceutical industry
In table 3, the experiment illumination is natural illumination, and the cutting seedlings are irradiated by natural astigmatism in the daytime, so that the direct irradiation of sunlight to the cutting seedlings is avoided.
The data in table 3 show that: the higher air temperature and water temperature are beneficial to the rooting of the cutting of the medical industrial hemp. The optimal environmental conditions are that the air temperature is 25-35 ℃, the water temperature is 20-25 ℃, the cutting seedlings are naturally irradiated in a scattered light manner in the daytime, and the direct irradiation of sunlight to the cutting seedlings is avoided.
Claims (3)
1. A method for water culture rooting of cutting seedlings of Cannabis sativa in pharmaceutical industry is characterized by comprising the following steps:
a, cultivating medicinal industrial hemp cutting seedlings by adopting a special water culture device to root, respectively containing water culture solution in an upper water tank (2) and a lower water tank (3) of the special water culture device, inserting cutting strips into planting holes (8) of a foam plate (1) floating on the water surface in the upper water tank (2), immersing the lower parts of the cutting strips in the water culture solution by 2-2.5 cm, selecting main branch top leaf spikes with stems being semi-lignified or lignified 8-15 cm long before the medicinal industrial hemp blooms, or side branch top leaf spikes with stems being semi-lignified or lignified 8-15 cm long, and trimming the main branch top leaf spikes or the side branch top leaf spikes, wherein the trimming is to only reserve spike tips and 1-2 leaves for the main branch top leaf spikes or the side branch top leaf spikes, and half leaves are cut off for each leaf; the water culture solution is tap water, sterilized water or distilled water;
step B, the water culture environmental conditions are as follows: the temperature is 25-35 ℃, the water temperature is 20-25 ℃, the illumination is natural light, and the cuttage seedlings are irradiated by natural astigmatism in the daytime;
c, the submersible pump is in an open working state in the whole water culture rooting process of the cutting seedlings, so that the water culture solution in the lower-layer water tank (3) is continuously pumped into the upper-layer water tank (2) through the submersible pump (4), and the water culture solution is sprayed to the leaf surfaces of the cutting seedlings 4-6 times every day;
the special water culture device has the structure that: comprises an upper layer of water tank, a lower layer of water tank (3) and an upper layer of water tank (2) which is nested in the lower layer of water tank (3) from bottom to top; a foam plate (1) is arranged in the upper layer water tank (2), and an overflow hole (7) is arranged on the upper tank wall of the upper layer water tank (2); water pumping holes (5) with the interval of 20-30cm are formed in the bottom of the upper-layer water tank (2), a submersible pump (4) is placed in the lower-layer water tank (3), a submersible pump water outlet pipe (6) penetrates through the water pumping holes (5) of the upper-layer water tank and penetrates through part of the upper-layer water tank in a groove body (11), and the contact part of the pipe wall of the submersible pump water outlet pipe (6) and the water pumping holes (5) is hermetically connected through a sealing ring (10); planting holes (8) with the interval of 4-6cm are arranged on the foam board (1); the nesting is that a clamping groove (9) is arranged at the edge of the top of the upper-layer water tank (2), the top of the lower-layer water tank (3) is inserted into the clamping groove (9), a tank body (11) of the upper-layer water tank is arranged in the inner cavity of the lower-layer water tank (3), and the bottom of the upper-layer water tank (2) is not contacted with a submersible pump (4) in the lower-layer water tank (3);
in the process of water culture rooting, the foam plate (1) is positioned below the overflow hole (7) or is parallel and level to the overflow hole (7) and is positioned above the submersible pump water outlet pipe (6) in the upper-layer water tank (2).
2. The method for rooting by water planting of cutting seedlings of Cannabis sativa in pharmaceutical industry according to claim 1, wherein the diameter of water-lifting hole (5) on the bottom surface of upper water tank (2) in the special water planting device is 1.8-2.2 cm; the aperture of the planting holes (8) on the foam board (1) is 2.0-2.5 cm.
3. The method for rooting by water culture of cutting seedlings of Cannabis sativa in pharmaceutical industry according to claim 1 or 2, wherein the depth of the upper water tank (2) is 7-10 cm, and the thickness of the foam board (1) is 1.0-3.0 cm.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112438195A (en) * | 2020-11-18 | 2021-03-05 | 福建省中科生物股份有限公司 | Industrial hemp water culture cuttage method |
| CN112772390A (en) * | 2021-01-11 | 2021-05-11 | 中国农业科学院麻类研究所 | Method for promoting industrial hemp cutting seedling water culture rooting |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112293240A (en) * | 2020-10-29 | 2021-02-02 | 信阳农林学院 | Soilless culture device |
| CN112438195A (en) * | 2020-11-18 | 2021-03-05 | 福建省中科生物股份有限公司 | Industrial hemp water culture cuttage method |
| CN112772390A (en) * | 2021-01-11 | 2021-05-11 | 中国农业科学院麻类研究所 | Method for promoting industrial hemp cutting seedling water culture rooting |
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| CN110972918B (en) | 2021-09-28 |
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