CN103026887A - Cutting propagation method for fibraurea recisa pierre - Google Patents
Cutting propagation method for fibraurea recisa pierre Download PDFInfo
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- CN103026887A CN103026887A CN2013100020299A CN201310002029A CN103026887A CN 103026887 A CN103026887 A CN 103026887A CN 2013100020299 A CN2013100020299 A CN 2013100020299A CN 201310002029 A CN201310002029 A CN 201310002029A CN 103026887 A CN103026887 A CN 103026887A
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Abstract
The invention discloses a cutting propagation method for a fibraurea recisa pierre. The method which is simple in operation, fast in rootage of a seedling and high in survival rate and can meet the requirement of producing a large quantity of seedlings in a short period of time is implemented through taking a young branch cut into segments as a material, sieving different concentrations of a rooting agent and the soak time, and regulating a small culture environment. By adopting the method, the problems of less seeds, long time for propagation of seeds and lack of the seedlings in production are solved, and the requirements of a vegetable drug enterprise on raw materials are met.
Description
Technical field
The invention belongs to plant seedling propagation technique field, be specifically related to the cuttage breeding method of rare and endangered medicinal plant huangteng.
Background technology
Huangteng (Fibraurea recisa Pierre), have another name called herba aristolochiae, herba fibraureae recisae, fibraurea recisa, be the perennial liane of Menispermaceae beauty Calamus, at China mainly distribute southeastern Yunnan, south Guangxi and Surface in Southwest Coastal Area of Guangdong, mainly be distributed in Vietnam, Burma, Laos and Cambodia abroad.Be born in dark and damp, fertile place in mountain valley, the hot-zone thick forest of height above sea level 200～700m, tendril often climbs on the large tree or precipice on its next door.Contain palmatine, jateorrhizine, jamaicin, fibranine, fibraminine, fibralactone and sterol etc. in huangteng root and the stem; Wherein palmatine has widely antibacterial action, and the tetrahydropalmatine that obtains through hydrogenation has analgesia, antispastic effect; Jateorrhizine has anesthesia and hypotensive effect to nervous centralis; Often be used to treat trauma infection contamination this plant among the people, the chronic ulcer that sore causes, having preferably, curative effect there is no obvious adverse reaction; Also be used for the treatment of simultaneously gynaecological imflammation, surgical infection, bacillary dysentery, enteritis, respiratory tract infection and eye conjunctivitis etc.
Because huangteng has important medical value, deeply is subjected to the world of medicine and patient's favorable comment.Since last century the seventies, successively built up in Yunnan and a plurality ofly to be engaged in the enterprise that produces with the huangteng autonomic drug, and purchased a large amount of huangteng raw material, wherein all derive from wild resource.Along with the continuous growth of demand, wild huangteng resource is reduced year by year, has been in Endangered status.Therefore, for satisfying the market to raw-material demand, the Cultivated Rheum rattan has become current necessary ways, however huangteng Plantlet formation seed is less, only can not satisfy Production requirement by seminal propagation, become the new way of producing seedling by branch cutting for this reason.
Summary of the invention
The object of the invention is to for the huangteng huge market demand, but the present situation that wild resource is deficient, seedling lacks in the production provides a kind of easy and simple to handle, huangteng seedling cuttage breeding method fast, that survival rate is high of taking root.
Purpose of the present invention is achieved by the following technical programs.
* except as otherwise noted, percentage of the present invention is mass percent.
The cuttage breeding method of a kind of huangteng may further comprise the steps:
(1) husky bed is prepared: make husky bed at the indoor clean river sand of temperature, with clear water the sand bed is irrigated, humidity reaches more than 90%;
(2) root-growing agent preparation: after getting indolebutyric acid and fully dissolving with absolute ethyl alcohol and clear water be mixed with the root-growing agent solution that indolebutyric acid concentration is 5～1500mg/L;
(3) transplant a cutting to prepare: in May～July, gather anosis worm harm, robust growth then or the branch in young age that sends the previous year, all blades are cut off, only keep the long petiole of 1～2cm; Each transplant a cutting to guarantee to have two joints, will transplant a cutting morphologic upper end to be cut into after the flat mouth and to seal after melting with paraffin or with candle, is cut into the angle of 20 °～45° angle with transplanting a cutting the morphology lower end, and the otch at two ends should be apart from joint 1～2cm length;
(4) transplant a cutting to soak: will transplant a cutting morphologic lower end is immersed in the root-growing agent solution, and solution is dipped into a cutting joint bottom at least;
(5) heel in: the cutting that will soak is heeled on the husky bed of step (1), and sprays husky bed with the carbendazim solution of 50～1000 times of liquid, to soak into whole husky bed as degree;
(6) seedbed management: at the agricultural film of the husky bed tiling layer of transparent of heeling in cutting, and will cover tightly around the film with river sand, raised afterwards film every 7 days and irrigate husky bed once with the carbendazim solution of 50～1000 times of liquid, again with on the film cover, water continuously three times; Husky bed tempertaure remains between 20～30 ℃ during this period, and humidity is controlled between 50～90%; Meanwhile, transplant a cutting and after husky bed is heeled in 7～15 days, opened some apertures with small wooden at film every 2～5 days, make the subenvironment of husky bed progressively near external environment condition;
(7) transplant: heel in the plantlet of transplant that will take root after 25～30 days to nutritious bag in, and move in the shed, irrigate with the carbendazim solution of 50～1000 times of liquid, watered once every 5～10 days subsequently, continue to water 3～5 times; The forest soil of matrix in the nutritious bag for not cultivating crops; Transplant that later on to carry out foliage-spray with composite fertilizer's filtrate of 0.05%～2.0% in 20 days be the plant strain growth extra-nutrition; Offspring to be generated was cultivated in nutritious bag after 30～60 days can be transplanted to the land for growing field crops.
Compared with prior art, the present invention has following beneficial effect:
Cutting medium in the present invention, transplant a cutting and need not to carry out separately sterilization, can heel in complete get final product with the carbendazim solution disposable bactericidal afterwards; The seedbed also need not to carry out precision management, and the process simple management can guarantee the rooting rate more than 95%; It is simple to operate, popularization is stronger, and most plantations can be grasped the operation of this technology per family, have reduced management cost; Can plant the family for huangteng by this technology a large amount of production seedlings are provided, also can indirectly provide sufficient raw material for autonomic drug manufacturing enterprise.
Embodiment
By specific embodiment given below; can the present invention is described in further detail; but be to be understood that; embodiment only is explanation of the invention; and limit the invention never in any form; various changes or modification that those skilled in the art's spirit according to the present invention is done fall within protection scope of the present invention equally.
Embodiment 1
In plastic tunnel of the Ping Bianxian of Yunnan Province, be the rectangle that the cement hollow fragment of brick of 25cm surrounds wide 1.0-1.5m with thickness in May, 2012, and length is with the physical condition in the quantity of cottage propagation seedling or greenhouse and decide, but should not be above 20 meters; The river sand that is about 0.05-0.2cm with particle diameter is again filled, and filling river sand thickness is 15-20cm; Need before using with running water whole husky bed to be irrigated, guarantee that the humidity when the cuttage branch reaches more than 90%, for subsequent use.
Take by weighing an amount of indolebutyric acid with analytical balance and also fully it is dissolved with a small amount of absolute ethyl alcohol, add again clear water to be mixed with concentration as the solution of 5-1500mg/L, for subsequent use.
From field acquisition then or the huangteng branch in young age of the anosis or worm that sends the previous year harm, robust growth, cut off all blades, only keep the approximately long petiole of 1-2cm, be cut into the cutting with two joints, being about to transplant a cutting morphologic upper end is cut into flat mouth and seals with paraffin or illuminating candle, and the morphology lower end being cut into the angle at 20-45 ° of angle, the otch at two ends should be long apart from joint 1-2cm.
The above-mentioned cutting morphology lower end that shears and seal is immersed in the indolebutyric acid solution, soak to take out behind 15 min and transplant a cutting and heel on the off-the-shelf husky bed by the specification of seeding row spacing 5cm * 10cm, with the carbendazim solution that dilutes 400 times husky bed is sprayed again, to soak into whole husky bed as degree.Cover tightly around the film afterwards at the agricultural film of the husky bed tiling layer of transparent of heeling in cutting, and with river sand, every 7 days film raised and irrigate husky bed once with carbendazim solution, again with on the film cover, water continuously carbendazim solution three times; Note during this period checking husky bed temperature humidity, temperature remains between 20-30 ℃, and humidity is controlled between the 50-90%.
The film on the husky bed was pierced through in cutting with small wooden every 2-5 days after husky bed is heeled in 7-15 days, form air-vent, the air-vent size be 1-5cm * 1-5cm(long * wide), the air-vent spacing is initially 2-5m * 2-5m, between two air-vents, opened again subsequently an aperture every 2-5 days, increase gradually the quantity of air-vent, make the subenvironment of husky bed progressively near external environment condition, last till when transplanting nutritious bag till.Continue in the nutritious bag to cultivate until can move on to after 25-30 days, the rooting rate that transplant a cutting this moment reaches more than 95%, and root length can reach 2-5cm.
The plantlet of transplant of having taken root in the nutritious bag that red soil is housed, and is placed in the semicircle little shed of height, the wide 1.5m of being respectively and 1m, irrigate with carbendazim solution afterwards, watered once every 5-10 days subsequently, continue to water 3-5 time; Transplant the composite fertilizer (N:P that used later on 0.05%-2.0% in 20 days
2O
5: K
2It is that plant strain growth supplements the nutrients that the filtrate of O=15:15:15) is carried out foliage-spray.Offspring to be generated was cultivated in nutritious bag after 30-60 days can be transplanted to the land for growing field crops.
Mainly obtain seedling with seminal propagation on producing at present, and huangteng needs the above plant of life at least 5 years just to have bearing capacity, and seed amount is few, on producing, only relies on seminal propagation to be difficult to the formation scale, seriously restricted the development of huangteng industry.And the whole process operation that adopts the branch cutting breeding is simple, popularization is stronger, and most plantations can be grasped the operation of this technology per family, have reduced management cost; The rooting of cuttings rate of treated mistake reaches more than 95%, can obtain fast a large amount of seedlings in a short time, for the huangteng industry development provides a favorable security.
Claims (3)
1. the cuttage breeding method of a huangteng may further comprise the steps:
(1) husky bed is prepared: make husky bed at the indoor clean river sand of temperature, with clear water the sand bed is irrigated, humidity reaches more than 90%;
(2) root-growing agent preparation: after getting indolebutyric acid and fully dissolving with absolute ethyl alcohol and clear water be mixed with the root-growing agent solution that indolebutyric acid concentration is 5～1500mg/L;
(3) transplant a cutting to prepare: in May～July, gathers anosis or worm harm, robust growth then or the branch in young age that sends the previous year, all blades are cut off, only keep the petiole that 1～2cm grows; Each transplant a cutting to guarantee to have two joints, will transplant a cutting morphologic upper end to be cut into after the flat mouth and to seal after melting with paraffin or with candle, is cut into the angle of 20 °～45° angle with transplanting a cutting the morphology lower end, and the otch at two ends should be apart from joint 1～2cm length;
(4) transplant a cutting to soak: will transplant a cutting morphologic lower end is immersed in the root-growing agent solution, and solution is dipped into a cutting joint bottom at least;
(5) heel in: the cutting that will soak is heeled on the husky bed of step (1), and sprays husky bed with the carbendazim solution of 50～1000 times of liquid, to soak into whole husky bed as degree;
(6) seedbed management: at the agricultural film of the husky bed tiling layer of transparent of heeling in cutting, and will cover tightly around the film with river sand, raised afterwards film every 7 days and irrigate husky bed once with the carbendazim solution of 50～1000 times of liquid, again with on the film cover, water continuously three times; Husky bed tempertaure remains between 20～30 ℃ during this period, and humidity is controlled between 50～90%; Meanwhile, transplant a cutting and after husky bed is heeled in 7～15 days, opened some apertures with small wooden at film every 2～5 days, make the subenvironment of husky bed progressively near external environment condition;
(7) transplant: heel in the plantlet of transplant that will take root after 25～30 days to nutritious bag in, and move in the shed, irrigate with the carbendazim solution of 50～1000 times of liquid, watered once every 5～10 days subsequently, continue to water 3～5 times; The forest soil of matrix in the nutritious bag for not cultivating crops; Transplant that later on to carry out foliage-spray with composite fertilizer's filtrate of 0.05%～2.0% in 20 days be the plant strain growth extra-nutrition; Offspring to be generated was cultivated in nutritious bag after 30～60 days can be transplanted to the land for growing field crops.
2. the cuttage breeding method of huangteng according to claim 1, it is characterized in that: the seeding row spacing of heeling between cutting described in the step (5) is 3～5cm * 10～15cm.
3. the cuttage breeding method of huangteng according to claim 1, it is characterized in that: in the step (7), described composite fertilizer filtrate is N:P
2O
5: K
2O=15:15:15 dissolves with clear water by 0.05%～2.0% concentration, constantly stirs in the course of dissolution, filters solid residue with hospital gauze at last, and the filtrate that obtains is as blade spraying.
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| CN2013100020299A CN103026887A (en) | 2013-01-05 | 2013-01-05 | Cutting propagation method for fibraurea recisa pierre |
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| CN2013100020299A CN103026887A (en) | 2013-01-05 | 2013-01-05 | Cutting propagation method for fibraurea recisa pierre |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105409487A (en) * | 2014-09-12 | 2016-03-23 | 紫云自治县燎原中药材产业化种植专业合作社 | Cuttage seedling propagation method for Sophora Subprostrata |
| CN105766352A (en) * | 2016-04-11 | 2016-07-20 | 西南林业大学 | Calamus plant cultivation method |
| CN106866271A (en) * | 2017-03-27 | 2017-06-20 | 湖南正清制药集团股份有限公司 | A kind of nutrient solution of promotion caulis sinomenii seedling root growth |
| CN107027483A (en) * | 2017-04-27 | 2017-08-11 | 临沧惠康中药材资源开发有限公司 | A kind of mating system of hymsleya amabilis |
| CN107306740A (en) * | 2017-07-31 | 2017-11-03 | 中国科学院西双版纳热带植物园 | A kind of indian pseudostreblus bark sapling multiplication method |
| CN108370677A (en) * | 2016-10-31 | 2018-08-07 | 昆明仟玺农业科技有限公司 | The implantation methods of huangteng |
| CN109691344A (en) * | 2019-03-08 | 2019-04-30 | 中国科学院西双版纳热带植物园 | A kind of indian pseudostreblus bark cuttage breeding method |
| CN111990079A (en) * | 2020-10-12 | 2020-11-27 | 湖南衡岳中药饮片有限公司 | Cutting seedling raising method for radix stephaniae tetrandrae |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105409487A (en) * | 2014-09-12 | 2016-03-23 | 紫云自治县燎原中药材产业化种植专业合作社 | Cuttage seedling propagation method for Sophora Subprostrata |
| CN105409487B (en) * | 2014-09-12 | 2018-08-24 | 紫云自治县燎原中药材产业化种植专业合作社 | A kind of cuttage and seedling culture method of subprostrate sophora |
| CN105766352A (en) * | 2016-04-11 | 2016-07-20 | 西南林业大学 | Calamus plant cultivation method |
| CN105766352B (en) * | 2016-04-11 | 2018-09-18 | 西南林业大学 | A kind of breeding method of Calamus plant |
| CN108370677A (en) * | 2016-10-31 | 2018-08-07 | 昆明仟玺农业科技有限公司 | The implantation methods of huangteng |
| CN106866271A (en) * | 2017-03-27 | 2017-06-20 | 湖南正清制药集团股份有限公司 | A kind of nutrient solution of promotion caulis sinomenii seedling root growth |
| CN107027483A (en) * | 2017-04-27 | 2017-08-11 | 临沧惠康中药材资源开发有限公司 | A kind of mating system of hymsleya amabilis |
| CN107306740A (en) * | 2017-07-31 | 2017-11-03 | 中国科学院西双版纳热带植物园 | A kind of indian pseudostreblus bark sapling multiplication method |
| CN109691344A (en) * | 2019-03-08 | 2019-04-30 | 中国科学院西双版纳热带植物园 | A kind of indian pseudostreblus bark cuttage breeding method |
| CN111990079A (en) * | 2020-10-12 | 2020-11-27 | 湖南衡岳中药饮片有限公司 | Cutting seedling raising method for radix stephaniae tetrandrae |
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Application publication date: 20130410 |