CN106866271A - A kind of nutrient solution of promotion caulis sinomenii seedling root growth - Google Patents

A kind of nutrient solution of promotion caulis sinomenii seedling root growth Download PDF

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CN106866271A
CN106866271A CN201710188450.1A CN201710188450A CN106866271A CN 106866271 A CN106866271 A CN 106866271A CN 201710188450 A CN201710188450 A CN 201710188450A CN 106866271 A CN106866271 A CN 106866271A
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nutrient solution
caulis sinomenii
seedling
promotion
mnso
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杨华
何莹
郭纯
熊兴耀
吴飞驰
曾茜垚
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ZHENGQING PHARMACEUTICAL GROUP CORP Ltd HUNAN PROV
Hunan Agricultural University
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ZHENGQING PHARMACEUTICAL GROUP CORP Ltd HUNAN PROV
Hunan Agricultural University
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers
    • C05G5/23Solutions
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • A01G31/02Special apparatus therefor
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B7/00Fertilisers based essentially on alkali or ammonium orthophosphates
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Environmental Sciences (AREA)
  • Hydroponics (AREA)
  • Fertilizers (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

A kind of nutrient solution of promotion caulis sinomenii seedling root growth, every liter of nutrient solution contains KNO3 0.346‑0.350g、NH4NO3 0.2910‑0.2914g、KH2PO4 0.0543‑0.0547g、CaCl2·2H2O 0.20‑0.24g、MgSO4·7H2O 0.183‑0.187g、MnSO4·4H2O 0.01113‑0.01117g、ZnSO4·7H2O 0.0041‑0.0045g、CuSO4·5H2O 0.0000123‑0.0000127g、H3BO3 0.0029‑0.0033g、Na2MoO4·2H2O 0.000123‑0.000127g、KI 0.000413‑0.000417g、CoCl2·6H2O 0.0000123‑0.0000127g、Na2The 0.01867g of EDTA 0.01863 and FeSO4·7H2O 0.0137‑0.0141g.Promotion caulis sinomenii seedling root growth nutrient solution water planting of the invention can quickly provide moisture and nutrition needed for seedling, can not only improve the rooting rate of material;The absorbability of root system can be strengthened simultaneously, improve the growth conditions of caulis sinomenii, shorten breeding time.

Description

A kind of nutrient solution of promotion caulis sinomenii seedling root growth
Technical field
The present invention relates to a kind of nutrient solution of promotion plant-root growth, and in particular to one kind promotes caulis sinomenii seedling root growth Nutrient solution.
Background technology
Caulis sinomenii also known as Sabia japonica, wind rattan, sinomenium acutum are sought, can wind-damp dispelling, degrading the channel, diuresis.Version in 2010《The Chinese people Republic's pharmacopeia》Regulation caulis sinomenii original plant is Menispermaceae root of fangji platymiscium sinomenium acutum Sinomenium acutum (Thumb) Rehd.et Wils and hair sinomenium acutum Sinomenium acutum (thumb) Rehd.et Wils.Var cinereum Rehd.Et Two kinds of Wils.
Caulis sinomenii is usually used in arthralgia pain due to rheumatism, and itch is benumbed in arthroncus;It is also used for renal glomerular disease, osteoproliferation, spinal cord Inflammation treatment;Also it is used for the treatment of the diseases such as arrhythmia cordis, erythematosus lupus and heroin dependence disease.It is clinical with its simple and Compound treatment rheumatic disease.Caulis sinomenii preparation on the market mainly has ZHENGQINGFENGTONGNING parenteral solution, ZHENGQINGFENGTONGNING to delay Release piece, rheumatic pain killing medicinal liquor, ZHENGQINGFENGTONGNING PIAN, Qishejingkang pills, chase after wind collaterals-activating capsule etc..
Caulis sinomenii, as a kind of Chinese medicine with unique drug effect, is always wild plant, rarely seen artificial cultivation.In recent years Come, with the disease such as rheumatism and rheumatoid arthritis, limbs pain numbness the incidence of disease be on the increase, the demand of caulis sinomenii Amount also increases therewith.The approximate madness of medicinal herb grower is disorderly excavated, and causes caulis sinomenii population to be cut down rapidly.Due to wild resource start it is deficient Weary, the partly pharmacy corporation with caulis sinomenii as raw material, such as Hunan Zheng Qing pharmacy group have started artificial cultivation caulis sinomenii.
Currently, plant vegetative propagation refers to be bred with vegetable nutritorium, including grafting, tissue cultures, cuttage etc.. It is wherein more convenient with cuttage, there is special meaning in the breeding of plant improved seeds.Cottage breeding offspring out, it is complete Site preparation, the good characteristic for solely remaining parent;The seed of the plant having in addition is little, it is difficult to largely obtain, a large amount of numerous Grow and also commonly use cuttage.But because many plant cuttages are difficult to take root, hinder this technology vegetative propagation field application and Promote.
The content of the invention
It is an object of the present invention to be directed to above-mentioned the deficiencies in the prior art, there is provided it is a kind of it is optimal, green grass or young crops can be rapidly promoted The nutrient solution of wind rattan seedling root growth, using nutrient solution of the invention, original soil cultivation is replaced using water planting, can shorten green grass or young crops Rootage duration in wind rattan cultivation and raising are taken root quantity, to promote the speed of growth of material, shorten growing-seedling period.
It is that, up to above-mentioned purpose, the technical solution adopted in the present invention is:A kind of nutrition of promotion caulis sinomenii seedling root growth Liquid, every liter of nutrient solution contains KNO3 0.346-0.350g、NH4NO3 0.2910-0.2914g、KH2PO40.0543-0.0547g、 CaCl2·2H2O 0.20-0.24g、MgSO4·7H2O 0.183-0.187g、MnSO4·4H2O0.01113-0.01117g、 ZnSO4·7H2O 0.0041-0.0045g、CuSO4·5H2O0.0000123-0.0000127g、H3BO3 0.0029- 0.0033g、Na2MoO4·2H2O0.000123-0.000127g、KI 0.000413-0.000417g、CoCl2· 6H2O0.0000123-0.0000127g、Na2- EDTA 0.01863-0.01867g and FeSO4·7H2O0.0137-0.0141g。
Preferably, every liter of nutrient solution contains KNO3 0.348g、NH4NO3 0.2912g、KH2PO4 0.0545g、CaCl2· 2H2O 0.22g、MgSO4·7H2O 0.185g、MnSO4·4H2O 0.01115g、ZnSO4·7H2O 0.0043g、CuSO4· 5H2O 0.0000125g、H3BO3 0.0031g、Na2MoO4·2H2O 0.000125g、KI 0.000415g、CoCl2·6H2O 0.0000125g、Na2- EDTA 0.01865g and FeSO4·7H2O 0.0139g。
The preparation method of above-mentioned nutrient solution:KNO is weighed respectively3、NH4NO3、KH2PO4、CaCl2·2H2O、MgSO4·7H2O、 MnSO4·4H2O、ZnSO4·7H2O、CuSO4·5H2O、H3BO3、Na2MoO4·2H2O、KI、CoCl2·6H2O、Na2- EDTA and FeSO4·7H2O, mixes, then be settled to 1000ml with distilled water after being dissolved respectively with distilled water.
Beneficial effects of the present invention are:Using water planting seedling method, caulis sinomenii seedling is entered into water-filling with nutrient solution of the invention Training, can quickly provide moisture and nutrition needed for seedling, simple to operate, with low cost, and operation can guarantee that seedling is normal in implementing Fast-growth and take root, grow seedling link shortens the root growth time compared with conventional soil cultivation, improves survival rate.
Specific embodiment
Technical scheme is described in further detail with reference to specific embodiment.
Analysis of the embodiment 1 to wild caulis sinomenii rhizosphere soil
Early stage shows that caulis sinomenii grows the demand ratio to N P and K to the information that the analysis of wild caulis sinomenii rhizosphere soil is obtained It is higher, specifically see table 1 below.
The situation of the caulis sinomenii rhizosphere soil N P and K of table 1
As can be seen from the above table, requirement of the caulis sinomenii to nitrogen and potassium is higher than general cultivated soil, it is contemplated that needs are added.
The influence that the nutrient solution of difference N, P, K content of embodiment 2 is taken root to caulis sinomenii seedling water planting 12d
Method:(1) seedling treatment:Excavation caulis sinomenii seedling of the same size, healthy growth, is cleaned soil with clear water Totally;
(2) sandstone treatment:Fine sand is rinsed well with clear water;
(3) prepared by nutrient solution:According to the information that the wild caulis sinomenii rhizosphere soil analysis of early stage is obtained, 9 groups of caulis sinomeniis are designed Nutrient solution:
1) No. 1 nutrient solution:Every liter of nutrient solution contains KNO30.2265g、NH4NO30.19555g、KH2PO40.0436g、 CaCl2·2H2O 0.22g、MgSO4·7H2O 0.185g、MnSO4·4H2O 0.01115g、ZnSO4·7H2O 0.0043g、 CuSO4·5H2O 0.0000125g、H3BO3 0.0031g、Na2MoO4·2H2O 0.000125g、KI 0.000415g、 CoCl2·6H2O 0.0000125g、Na2- EDTA 0.01865g and FeSO4·7H2O 0.0139g;
2) No. 2 nutrient solutions:Every liter of nutrient solution contains KNO30.3480g、NH4NO30.147g、KH2PO40.0545g、CaCl2· 2H2O 0.22g、MgSO4·7H2O 0.185g、MnSO4·4H2O 0.01115g、ZnSO4·7H2O 0.0043g、CuSO4· 5H2O 0.0000125g、H3BO3 0.0031g、Na2MoO4·2H2O 0.000125g、KI 0.000415g、CoCl2·6H2O 0.0000125g、Na2- EDTA 0.01865g and FeSO4·7H2O 0.0139g;
3) No. 3 nutrient solutions:Every liter of nutrient solution contains KNO30.2744g、NH4NO30.177g、KH2PO40.0654g、CaCl2· 2H2O 0.22g、MgSO4·7H2O 0.185g、MnSO4·4H2O 0.01115g、ZnSO4·7H2O 0.0043g、CuSO4· 5H2O 0.0000125g、H3BO3 0.0031g、Na2MoO4·2H2O 0.000125g、KI 0.000415g、CoCl2·6H2O 0.0000125g、Na2- EDTA 0.01865g and FeSO4·7H2O 0.0139g;
4) No. 4 nutrient solutions:Every liter of nutrient solution contains KNO30.35595g、NH4NO30.21555g、KH2PO4 0.0436g、 CaCl2·2H2O 0.22g、MgSO4·7H2O 0.185g、MnSO4·4H2O 0.01115g、ZnSO4·7H2O 0.0043g、 CuSO4·5H2O 0.0000125g、H3BO3 0.0031g、Na2MoO4·2H2O 0.000125g、KI 0.000415g、 CoCl2·6H2O 0.0000125g、Na2- EDTA 0.01865g and FeSO4·7H2O 0.0139g;
5) No. 5 nutrient solutions:Every liter of nutrient solution contains KNO30.28345g、NH4NO30.24405g、KH2PO4 0.0545g、 CaCl2·2H2O 0.22g、MgSO4·7H2O 0.185g、MnSO4·4H2O 0.01115g、ZnSO4·7H2O 0.0043g、 CuSO4·5H2O 0.0000125g、H3BO3 0.0031g、Na2MoO4·2H2O 0.000125g、KI 0.000415g、 CoCl2·6H2O 0.0000125g、Na2- EDTA 0.01865g and FeSO4·7H2O 0.0139g;
6) No. 6 nutrient solutions:Every liter of nutrient solution contains KNO30.20965g、NH4NO30.27405g、KH2PO40.0654g、 CaCl2·2H2O 0.22g、MgSO4·7H2O 0.185g、MnSO4·4H2O 0.01115g、ZnSO4·7H2O 0.0043g、 CuSO4·5H2O 0.0000125g、H3BO3 0.0031g、Na2MoO4·2H2O 0.000125g、KI 0.000415g、 CoCl2·6H2O 0.0000125g、Na2- EDTA 0.01865g and FeSO4·7H2O 0.0139g;
7) No. 7 nutrient solutions:Every liter of nutrient solution contains KNO30.2912g、NH4NO30.3126g、KH2PO40.0436g、CaCl2· 2H2O 0.22g、MgSO4·7H2O 0.185g、MnSO4·4H2O 0.01115g、ZnSO4·7H2O 0.0043g、CuSO4· 5H2O 0.0000125g、H3BO3 0.0031g、Na2MoO4·2H2O 0.000125g、KI 0.000415g、CoCl2·6H2O 0.0000125g、Na2- EDTA 0.01865g and FeSO4·7H2O 0.0139g;
8) No. 8 nutrient solutions:Every liter of nutrient solution contains KNO30.21875g、NH4NO30.34115g、KH2PO40.0545g、 CaCl2·2H2O 0.22g、MgSO4·7H2O 0.185g、MnSO4·4H2O 0.01115g、ZnSO4·7H2O 0.0043g、 CuSO4·5H2O 0.0000125g、H3BO3 0.0031g、Na2MoO4·2H2O 0.000125g、KI 0.000415g、 CoCl2·6H2O 0.0000125g、Na2- EDTA 0.01865g and FeSO4·7H2O 0.0139g;
9) No. 9 nutrient solutions:Every liter of nutrient solution contains KNO30.3391g、NH4NO30.2912g、KH2PO40.0654g、CaCl2· 2H2O 0.22g、MgSO4·7H2O 0.185g、MnSO4·4H2O 0.01115g、ZnSO4·7H2O 0.0043g、CuSO4· 5H2O 0.0000125g、H3BO3 0.0031g、Na2MoO4·2H2O 0.000125g、KI 0.000415g、CoCl2·6H2O 0.0000125g、Na2- EDTA 0.01865g and FeSO4·7H2O 0.0139g。
(4) cultivate:The aperture for cultivating tray bottom is blocked with the small scraps of paper, culture plate fills fine sand, then by clean green grass or young crops Wind rattan seedling kind is finally placed on culture plate in the pallet equipped with different culture formula of liquid in fine sand, covers culture lid, does Good mark;
(5) condition control:Culture materials are placed in illumination box and are cultivated, the condition of culture of illumination box is temperature 22-28 DEG C of degree, intensity of illumination 2000Lx and daily illumination in 12 hours.
As a result:The influence that the nutrient solution of different N, P, K contents is taken root to caulis sinomenii seedling water planting 12d, is shown in Table 2.
The material of each nutrient solution culture of table 2 is taken root situation
Analysis:Caulis sinomenii seedling is cultivated with above-mentioned nutrient solution, the growing way of caulis sinomenii root is periodically investigated.Data are carried out orthogonal Analysis, as a result shows, works as KNO3 0.348g、NH4NO3 0.2912g、KH2PO4 0.0545g、CaCl2·2H2O 0.22g、 MgSO4·7H2O 0.185g、MnSO4·4H2O 0.01115g、ZnSO4·7H2O 0.0043g、CuSO4·5H2O 0.0000125g、H3BO3 0.0031g、Na2MoO4·2H2O 0.000125g、KI 0.000415g、CoCl2·6H2O 0.0000125g、Na2- EDTA 0.01865g and FeSO4·7H2The hydroponic caulis sinomenii root configured during O 0.0139g Quantity is more and long, and growth is vigorous, energetic.
Embodiment 3
KNO is weighed respectively3 0.348g、NH4NO3 0.2912g、KH2PO4 0.0545g、CaCl2·2H2O 0.22g、 MgSO4·7H2O 0.185g、MnSO4·4H2O 0.01115g、ZnSO4·7H2O 0.0043g、CuSO4· 5H2O0.0000125g、H3BO3 0.0031g、Na2MoO4·2H2O 0.000125g、KI 0.000415g、CoCl2· 6H2O0.0000125g、Na2- EDTA 0.01865g and FeSO4·7H2O 0.0139g, mix after being dissolved respectively with distilled water, 1000ml is settled to distilled water again, that is, nutrient solution of the invention is obtained.With the Solution culture method caulis sinomenii seedling, caulis sinomenii children The situation that seedling water planting 12d is taken root, is shown in Table 3.
The situation of taking root of the Solution culture method material of the present invention of table 3
Nutrient solution Rooting rate % Always take root number (root) Take root material average total root it is (cm) long
Nutrient solution of the present invention 64.8 54 21.6
Analysis:With above-mentioned nutrient fluid cultivation caulis sinomenii seedling, the growing way of root is periodically investigated.As known from Table 3, present invention culture , for 9 tissue culture nutrient solutions in embodiment 2, the caulis sinomenii seedling rooting rate highest of culture, number of taking root is most, material growing way for liquid phase It is best.
Embodiment 4
KNO is weighed respectively3 0.346g、NH4NO3 0.2910g、KH2PO4 0.0.0543g、CaCl2·2H2O 0.20g、 MgSO4·7H2O 0.183g、MnSO4·4H2O 0.01113g、ZnSO4·7H2O 0.0041g、CuSO4· 5H2O0.0000123g、H3BO3 0.0029g、Na2MoO4·2H2O 0.000123g、KI 0.000413g、CoCl2· 6H2O0.0000123g、Na2- EDTA 0.01863g and FeSO4·7H2O 0.0137g, mix after being dissolved respectively with distilled water, 1000ml is settled to distilled water again, nutrient solution of the invention is obtained final product.
Embodiment 5
KNO is weighed respectively3 0.350g、NH4NO3 0.2914g、KH2PO4 0.0547g、CaCl2·2H2O 0.24g、 MgSO4·7H2O 0.187g、MnSO4·4H2O 0.01117g、ZnSO4·7H2O 0.0045g、CuSO4· 5H2O0.0000127g、H3BO3 0.0033g、Na2MoO4·2H2O 0.000127g、KI 0.000417g、CoCl2· 6H2O0.0000127g、Na2- EDTA 0.01867g and FeSO4·7H2O 0.0141g, mix after being dissolved respectively with distilled water, 1000ml is settled to distilled water again, nutrient solution of the invention is obtained final product.

Claims (2)

1. a kind of nutrient solution for promoting caulis sinomenii seedling root growth, it is characterised in that every liter of nutrient solution contains KNO3 0.346- 0.350g、NH4NO3 0.2910-0.2914g、KH2PO4 0.0543-0.0547g、CaCl2·2H2O 0.20-0.24g、 MgSO4·7H2O 0.183-0.187g、MnSO4·4H2O 0.01113-0.01117g、ZnSO4·7H2O 0.0041- 0.0045g、CuSO4·5H2O 0.0000123-0.0000127g、H3BO3 0.0029-0.0033g、Na2MoO4·2H2O 0.000123-0.000127g、KI 0.000413-0.000417g、CoCl2·6H2O 0.0000123-0.0000127g、Na2- EDTA 0.01863-0.01867g and FeSO4·7H2O 0.0137-0.0141g。
2. a kind of nutrient solution for promoting caulis sinomenii seedling root growth as claimed in claim 1, it is characterised in that every liter of nutrient solution Containing KNO3 0.348g、NH4NO3 0.2912g、KH2PO4 0.0545g、CaCl2·2H2O 0.22g、MgSO4·7H2O 0.185g、MnSO4·4H2O 0.01115g、ZnSO4·7H2O 0.0043g、CuSO4·5H2O 0.0000125g、H3BO3 0.0031g、Na2MoO4·2H2O 0.000125g、KI 0.000415g、CoCl2·6H2O 0.0000125g、Na2-EDTA 0.01865g and FeSO4·7H2O 0.0139g。
CN201710188450.1A 2017-03-27 2017-03-27 A kind of nutrient solution of promotion caulis sinomenii seedling root growth Pending CN106866271A (en)

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CN107568047A (en) * 2017-09-20 2018-01-12 中国农业科学院柑桔研究所 For evaluating the liquid film ciltivating process of citrus resistance
CN107568047B (en) * 2017-09-20 2020-09-22 中国农业科学院柑桔研究所 Liquid membrane water culture method for evaluating stress resistance of citrus
CN108684346A (en) * 2018-05-03 2018-10-23 贵阳中医学院 A kind of cuttage breeding method of Sabia parviflora Wall.ex Roxb

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Application publication date: 20170620