CN107278758A - The breeding method of sea-buckthorn seedling on a kind of salt-soda soil - Google Patents

The breeding method of sea-buckthorn seedling on a kind of salt-soda soil Download PDF

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Publication number
CN107278758A
CN107278758A CN201710406804.5A CN201710406804A CN107278758A CN 107278758 A CN107278758 A CN 107278758A CN 201710406804 A CN201710406804 A CN 201710406804A CN 107278758 A CN107278758 A CN 107278758A
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sea
buckthorn
salt
culture hole
soil
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毛吉贤
郭飞
胡芳
赵亮
韩顺涛
叶紫
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05CNITROGENOUS FERTILISERS
    • C05C3/00Fertilisers containing other salts of ammonia or ammonia itself, e.g. gas liquor
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80Soil conditioners

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Soil Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Fertilizers (AREA)

Abstract

A kind of breeding method of sea-buckthorn seedling on salt-soda soil, material includes:Cinder, ardealite, ammonium sulfate, Concave-convex clay rod, wormcast, easily farm manure, decomposition sponge, bacillus grease, soil, the labile starch film for being adapted to sea-buckthorn growth of seedling;The present invention is easy and effective to the breeding method of sea-buckthorn seedling, it is pollution-free, and whole improvement need not be carried out to the salt-soda soil of large area, cost and the energy are saved, the present invention can be such that the sea-buckthorn seedling of plantation is largely survived on salt-soda soil, sea-buckthorn after surviving can carry out effective improvement after raised growth is bred to salt-soda soil, while the fruit that the sea-buckthorn after growth adult is born has very high economic value.

Description

The breeding method of sea-buckthorn seedling on a kind of salt-soda soil
Technical field
The present invention relates to the improving technology field in salt-soda soil, and in particular on a kind of salt-soda soil the cultivation side of sea-buckthorn seedling Method.
Background technology
Salt-soda soil refers to that the salt alkali number contained by inside soil has influence on the normal growth of crop, according to the United Nations's education, science and culture group Knit with food and agricultural organization's incomplete statistics, the area in whole world salt-soda soil is 9.5438 hundred million hectares, and wherein China is 99,130,000 hectares. The formation of China's alkaline earth and alkali-affected soil, it is most of relevant with the accumulation of Carbonates In Soil, and basicity is universal higher, seriously Salt affected soil regional plant can hardly survive.Various saline-alkali soil are formed under certain natural conditions, and it is formed Substantive reason be mainly various ease of solubility salts and be dissolved in after water with the evaporation and flowing of moisture, make on ground horizontal direction with Vertical direction is redistributed, so that upper soll layer of the salinity in collection salt area is built up.For changing for salt-soda soil Good, chemical modifying method has preferable effect within the shorter time limit time limit, and chemical modifying is exactly to apply some bisalts Material is micro- in the property in salt-soda soil, the cation replacement ability of increase soil, the salt content of reduction soil, enhancing soil to improve The activity of biological and enzyme, promotes plant root growth.Improve the physical property of soil, increase the crumb structure of soil, coordinate soil Earth liquid manure gas heat, increases soil fertility.But chemical modifying has chemical composition residual, scale is big, cost is high, it is longer etc. to take Shortcoming, simultaneously as it is that the saline and alkaline flowing with moisture in underground is assembled in earth's surface that salt-soda soil, which is formed, by chemical modifying Soil is general can be with planting plants in the first two years, but have crossed after 2 to three years, and the salt-soda soil after improvement will enter one Step is returned original salt-soda soil, is made improvement not thorough by salinization of soil, pays very high financial cost and can not obtain for a long time Economy return, and chemical modifying cost far beyond the economy return after improvement.
At present, some have the plant of economic value and health value can be in saline and alkaline aerial, such as plant Sea-buckthorn.Plant sea-buckthorn is Elaeangnaceae Hippophne, is a kind of fallen leaves property shrub, and the sea-buckthorn of adult has drought-resistant, anti-blown sand, can It with the characteristic survived on wetland with saline-alkaline, and can gradually change the physical characteristic of soil, soil texture is changed, Quality becomes loose, breathes freely and water storage capacity enhancing, therefore be widely used in water and soil conservation.Sea-buckthorn is medicinal and edible plant.It is husky The root of spine, stem, leaf, flower, fruit, particularly sea buckthorn fruit contain abundant nutriment and bioactive substance, can answer extensively Many fields for national economy such as food, medicine, light industry, space flight, farming, animal husbandry and fishery.Sea buckthorn fruit is used as medicine with cough-relieving The effect of phlegm, stomach strengthening and digestion promoting, promoting blood circulation to remove blood stasis.Modern medicine study, sea-buckthorn can reduce cholesterol, and allevating angina pectoris breaking-out also has Prevent and treat the effect of coronary atherosclerotic heart disease.
Ripe sea-buckthorn can improve in saline and alkaline aerial and by amount reproduction root system to salt-soda soil, China north There is substantial amounts of salt-soda soil and can not be exploited in portion area, these salt-soda soils can be improved and be opened by planting sea-buckthorn Hair is utilized, but by substantial amounts of research and experiment find sea-buckthorn after field planting in two years, grown with underground based on, aerial part It is slow-growing, and the salt tolerant basicity in sea-buckthorn seedling period is not strong, is planted by conventional implantation methods on salt-soda soil The survival rate of seedling sea-buckthorn is very low, the seedling sea-buckthorn of plantation can not survive be puzzlement sea-buckthorn can not on salt-soda soil amount reproduction Main cause, and if planted again behind large area improvement salt-soda soil, it is necessary to pay huge economy and artificial input, mesh The preceding effective ways that transplanting sea-buckthorn improved soil is also carried out not on salt-soda soil.
The content of the invention
It is an object of the invention to provide a kind of breeding method of sea-buckthorn seedling on salt-soda soil, salt-soda soil is carried out by part Initial optimization and saline and alkaline flowing is locally isolated, so as to obtain after local optimum and the culture of 1 to two years high-quality soils can be kept Cheat soil, it is ensured that the sea-buckthorn seedling initially planted can largely be survived on salt-soda soil and after raised growth breeding root system to saline and alkaline Ground effectively utilize and improve.
The breeding method of sea-buckthorn seedling on a kind of salt-soda soil, it is characterised in that comprise the following steps:
(1) get the raw materials ready:Cinder, ardealite, ammonium sulfate, Concave-convex clay rod, wormcast, easily farm manure, decomposition sponge, bacillus Grease, soil, the labile starch film for being adapted to sea-buckthorn growth of seedling;
(2) a length of 80~120cm, a width of 60~100cm, the culture of a height of 50~90cm cuboid are first dug on salt-soda soil Hole, then cinder is ground into 100~200 targeted fine powders, it is laid in culture hole, its thickness is 5~8cm;Phosphogypsum powder is broken into The coarse powder of 50~80 mesh, is mixed with ammonium sulfate according to mass ratio 1: 2, be mixed thing pour into culture hole, its thickness 5~ 10cm;Mass percent is poured into culture hole for 10~20% Concave-convex clay rod again, its thickness is 4~8cm;Finally will Wormcast is mixed with farm manure according to mass ratio 1: 1, is poured into culture hole, and its thickness is 7~15cm;
(3) labile starch film is taped against on the inwall of culture hole surrounding, and on the surface of labile starch film Coat bacillus grease;Thickness is taped against on labile starch film for 3~5cm easy decomposition water-absorbing sponge again, and Bacillus grease is coated on the surface for easily decomposing water-absorbing sponge;The soil that suitable sea-buckthorn seedling grows up is filled up into culture hole, i.e., Watering Irrigation finally can be carried out to sea-buckthorn seedling culture hole by sea-buckthorn seedling replanting of the root system with parent-soil to culture hole.
Further, in the step (1), the content of bacillus is not less than 2,000,000,000 cfu/g in bacillus grease.
Further, in the step (3), the area for the labile starch film being taped against on the surrounding inwall of culture hole is equal to The area of culture hole surrounding, is taped against area of the area equal to culture hole surrounding that sponge is easily decomposed on labile starch film.
The beneficial effects of the present invention are:
The present invention carries out initial optimization to salt-soda soil by part and is locally isolated saline and alkaline flowing, so as to obtain after local optimum And the culture hole soil of 1 to two years high-quality soils can be kept, it is ensured that the sea-buckthorn seedling initially planted can largely be survived on salt-soda soil And effective utilization and improvement is carried out to salt-soda soil after raised growth breeds root system.The present invention is according to substantial amounts of in salt-soda soil The experimental data of cultivation sea-buckthorn in culture hole, it is ensured that water-absorbing sponge is easily decomposed in culture hole can provide early stage life for sea-buckthorn seedling Long required moisture, while the brushing bacillus grease on labile starch film and easily decomposition water-absorbing sponge, makes easy point The starch film of solution and easily decomposition water-absorbing sponge are completed one to decomposition in two years.Breeding method letter of the present invention to sea-buckthorn seedling It is single effective, it is pollution-free, and whole improvement need not be carried out to the salt-soda soil of large area, cost and the energy are saved, the present invention The sea-buckthorn seedling of plantation can be made largely to be survived on salt-soda soil, the sea-buckthorn after surviving can be to salt-soda soil after raised growth is bred Effectively improved, while the fruit that the sea-buckthorn after growth adult is born has very high economic value.
Embodiment
Example 1
The breeding method of sea-buckthorn seedling on a kind of salt-soda soil, it is characterised in that comprise the following steps:
(1) get the raw materials ready:Cinder, ardealite, ammonium sulfate, Concave-convex clay rod, wormcast, easily farm manure, decomposition sponge, bacillus Grease, soil, the labile starch film for being adapted to sea-buckthorn growth of seedling;
(2) a length of 80cm, a width of 60cm, the culture hole of a height of 50cm cuboid are first dug on salt-soda soil, then cinder is crushed Into 100 targeted fine powders, it is laid in culture hole, its thickness is 5cm;Phosphogypsum powder is broken into the coarse powder of 50 mesh, with ammonium sulfate according to Mass ratio 1: 2 is mixed, and is mixed thing and is poured into culture hole, its thickness is 5cm;Again by mass percent be 10% bumps Rod stone clay is poured into culture hole, and its thickness is 4cm;Finally wormcast is mixed with farm manure according to mass ratio 1: 1, training is poured into Support in hole, its thickness is 7cm;
(3) labile starch film is taped against on the inwall of culture hole surrounding, and on the surface of labile starch film Coat bacillus grease;Thickness is taped against on labile starch film for 3cm easy decomposition water-absorbing sponge again, and easy Coat bacillus grease in the surface for decomposing water-absorbing sponge;The soil that suitable sea-buckthorn seedling grows up is filled up into culture hole, you can will Sea-buckthorn seedling replanting of the root system with parent-soil is interior to culture hole, finally carries out watering Irrigation to sea-buckthorn seedling culture hole.
Example 2
The breeding method of sea-buckthorn seedling on a kind of salt-soda soil, it is characterised in that comprise the following steps:
(1) get the raw materials ready:Cinder, ardealite, ammonium sulfate, Concave-convex clay rod, wormcast, easily farm manure, decomposition sponge, bacillus Grease, soil, the labile starch film for being adapted to sea-buckthorn growth of seedling;
(2) a length of 100cm, a width of 80cm, the culture hole of a height of 70cm cuboid are first dug on salt-soda soil, then cinder is crushed Into 150 targeted fine powders, it is laid in culture hole, its thickness is 7cm;Phosphogypsum powder is broken into the coarse powder of 70 mesh, with ammonium sulfate according to Mass ratio 1: 2 is mixed, and is mixed thing and is poured into culture hole, its thickness 8cm;Again by mass percent be 15% concave convex rod Stone clay is poured into culture hole, and its thickness is 6cm;Finally wormcast is mixed with farm manure according to mass ratio 1: 1, culture is poured into In hole, its thickness is 12cm;
(3) labile starch film is taped against on the inwall of culture hole surrounding, and on the surface of labile starch film Coat bacillus grease;Thickness is taped against on labile starch film for 4cm easy decomposition water-absorbing sponge again, and easy Coat bacillus grease in the surface for decomposing water-absorbing sponge;The soil that suitable sea-buckthorn seedling grows up is filled up into culture hole, you can will Sea-buckthorn seedling replanting of the root system with parent-soil is interior to culture hole, finally carries out watering Irrigation to sea-buckthorn seedling culture hole.
Example 3
The breeding method of sea-buckthorn seedling on a kind of salt-soda soil, it is characterised in that comprise the following steps:
(1) get the raw materials ready:Cinder, ardealite, ammonium sulfate, Concave-convex clay rod, wormcast, easily farm manure, decomposition sponge, bacillus Grease, soil, the labile starch film for being adapted to sea-buckthorn growth of seedling;
(2) first a length of 120cm is dug on salt-soda soil, a width of 100cm, the culture hole of a height of 90cm cuboid, then by cinder powder 200 targeted fine powders are broken into, are laid in culture hole, its thickness is 8cm;Phosphogypsum powder is broken into the coarse powder of 80 mesh, is pressed with ammonium sulfate Mixed according to mass ratio 1: 2, be mixed thing and pour into culture hole, its thickness 10cm;Again by mass percent for 20% it is recessed Convex rod stone clay is poured into culture hole, and its thickness is 8cm;Finally wormcast is mixed with farm manure according to mass ratio 1: 1, poured into In culture hole, its thickness is 15cm;
(3) labile starch film is taped against on the inwall of culture hole surrounding, and on the surface of labile starch film Coat bacillus grease;Thickness is taped against on labile starch film for 5cm easy decomposition water-absorbing sponge again, and easy Coat bacillus grease in the surface for decomposing water-absorbing sponge;The soil that suitable sea-buckthorn seedling grows up is filled up into culture hole, you can will Sea-buckthorn seedling replanting of the root system with parent-soil is interior to culture hole, finally carries out watering Irrigation to sea-buckthorn seedling culture hole.

Claims (3)

1. the breeding method of sea-buckthorn seedling on a kind of salt-soda soil, it is characterised in that comprise the following steps:
(1) get the raw materials ready:Cinder, ardealite, ammonium sulfate, Concave-convex clay rod, wormcast, easily farm manure, decomposition sponge, bacillus Grease, soil, the labile starch film for being adapted to sea-buckthorn growth of seedling;
(2) a length of 80~120cm, a width of 60~100cm, the culture of a height of 50~90cm cuboid are first dug on salt-soda soil Hole, then cinder is ground into 100~200 targeted fine powders, it is laid in culture hole, its thickness is 5~8cm;Ardealite is crushed Into the coarse powder of 50~80 mesh, mixed with ammonium sulfate according to mass ratio 1: 2, be mixed thing pour into culture hole, its thickness 5~ 10cm;Mass percent is poured into culture hole for 10~20% Concave-convex clay rod again, its thickness is 4~8cm;Finally will Wormcast is mixed with farm manure according to mass ratio 1: 1, is poured into culture hole, and its thickness is 7~15cm;
(3) labile starch film is taped against on the inwall of culture hole surrounding, and on the surface of labile starch film Coat bacillus grease;Thickness is taped against on labile starch film for 3~5cm easy decomposition water-absorbing sponge again, and Bacillus grease is coated on the surface for easily decomposing water-absorbing sponge;The soil that suitable sea-buckthorn seedling grows up is filled up into culture hole, i.e., Watering Irrigation finally can be carried out to sea-buckthorn seedling culture hole by sea-buckthorn seedling replanting of the root system with parent-soil to culture hole.
2. the breeding method of sea-buckthorn seedling on a kind of salt-soda soil according to claim 1, it is characterised in that:The step (3) in, the content of bacillus is not less than 2,000,000,000 cfu/g in bacillus grease.
3. the breeding method of sea-buckthorn seedling on a kind of salt-soda soil according to claim 1, it is characterised in that:The step (3) in, the area for the labile starch film being taped against on the surrounding inwall of culture hole is equal to the area of culture hole surrounding, is taped against easily The area that sponge is easily decomposed on the starch film of decomposition is equal to the area of culture hole surrounding.
CN201710406804.5A 2017-05-14 2017-05-14 The breeding method of sea-buckthorn seedling on a kind of salt-soda soil Pending CN107278758A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108307913A (en) * 2018-03-21 2018-07-24 江孜县林业局 Hippophne seedling plants breeding method
CN109247179A (en) * 2018-10-09 2019-01-22 江苏省中国科学院植物研究所 A method of in salt-soda soil, plantation angstrom is than thorny elaeagnus
CN114752112A (en) * 2022-04-29 2022-07-15 贵州开磷磷石膏综合利用有限公司 Application of modified phosphogypsum in preparation of degradable flower mud

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102498837A (en) * 2011-09-30 2012-06-20 山东胜伟园林科技有限公司 Seedling planting method in alkaline land
CN202489027U (en) * 2012-03-27 2012-10-17 山东胜伟园林科技有限公司 Garden greening water storage device
CN103131424A (en) * 2013-03-13 2013-06-05 刘海崇 Saline alkali soil conditioner
CN103275731A (en) * 2013-06-17 2013-09-04 新疆农业科学院土壤肥料与农业节水研究所 Saline-alkali soil modifier and preparation method and application thereof
CN103937507A (en) * 2014-03-31 2014-07-23 高程华 Saline-alkali soil improver as well as preparation method and use method thereof
CN104449757A (en) * 2014-12-01 2015-03-25 安徽养禾天生物科技有限公司 Modifier for saline-alkali soil as well as production method and application method of modifier

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102498837A (en) * 2011-09-30 2012-06-20 山东胜伟园林科技有限公司 Seedling planting method in alkaline land
CN202489027U (en) * 2012-03-27 2012-10-17 山东胜伟园林科技有限公司 Garden greening water storage device
CN103131424A (en) * 2013-03-13 2013-06-05 刘海崇 Saline alkali soil conditioner
CN103275731A (en) * 2013-06-17 2013-09-04 新疆农业科学院土壤肥料与农业节水研究所 Saline-alkali soil modifier and preparation method and application thereof
CN103937507A (en) * 2014-03-31 2014-07-23 高程华 Saline-alkali soil improver as well as preparation method and use method thereof
CN104449757A (en) * 2014-12-01 2015-03-25 安徽养禾天生物科技有限公司 Modifier for saline-alkali soil as well as production method and application method of modifier

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
宋德禄: "盐碱地营造沙棘林技术研究", 《资源建设与保护》 *
胡景文等: "吉林西北部盐碱地沙棘育苗技术", 《防护林科技》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108307913A (en) * 2018-03-21 2018-07-24 江孜县林业局 Hippophne seedling plants breeding method
CN109247179A (en) * 2018-10-09 2019-01-22 江苏省中国科学院植物研究所 A method of in salt-soda soil, plantation angstrom is than thorny elaeagnus
CN114752112A (en) * 2022-04-29 2022-07-15 贵州开磷磷石膏综合利用有限公司 Application of modified phosphogypsum in preparation of degradable flower mud
CN114752112B (en) * 2022-04-29 2024-03-19 贵州开磷磷石膏综合利用有限公司 Application of modified phosphogypsum in preparation of degradable flower mud

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