CN103548553A - Cutting propagation method of Marsdenia tenocissima (Roxb) Wight et Arm - Google Patents
Cutting propagation method of Marsdenia tenocissima (Roxb) Wight et Arm Download PDFInfo
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Abstract
The invention discloses a cutting propagation method of Marsdenia tenocissima (Roxb) Wight et Arm. The method comprises the steps as follows: a vinyl house is constructed in a reddish yellow soil area with an altitude of 800 m-1,000 m; lignified Marsdenia tenocissima(Roxb)Wight et Arm stems with more than three years of growth is picked in March to May, soaked in a 300mg/L IBA (indole butyric acid) solution after disinfection and sterilization, and then inserted in a seedbed; a sun shading mesh is constructed above the seedbed; in a first week after cuttage, the matrix humidity is controlled to be 90%-95%, the daytime temperature is controlled to be 25 DEG C- 34 DEG C, and the nocturnal temperature is controlled to be 20 DEG C-24 DEG C; in a second week, the matrix humidity is controlled to be 80%-89%, the daytime temperature is controlled to be 30 DEG C- 35 DEG C, and the nocturnal temperature is controlled to be 25 DEG C-28 DEG C; from a third week, the matrix humidity is controlled to be 60%-70%, the daytime temperature and the nocturnal temperature are controlled within 20 DEG C-35 DEG C; during the cuttage, ventilation is performed for half an hour every morning and 5 hours every noon until acclimatization and transplant after rooting of cuttings. According to the method, the cutting wood rooting percentage of the Marsdenia tenocissima(Roxb)Wight et Arm can reach 85%-93%, and the transplant survival rate can be higher than 92%.
Description
Technical field
The invention belongs to plant asexual multiplication technology field, be specifically related to the asexual cuttage breeding method of a kind of traditional Chinese medicine CAULIS MARSDENIAE TENACISSIMAE.
Background technology
CAULIS MARSDENIAE TENACISSIMAE (Marsdenia tenocissima (Roxb) Wight et Arm) is Asclepiadaceae Marsdenia liane, and its stem, root, leaf have anti-inflammatory, the effect such as clearing heat and detoxicating.The contained C21 steroid compound of CAULIS MARSDENIAE TENACISSIMAE has the ability of rooting out the lively power of tumour cell mitogen, take it as the Chinese medicine Xiaoaiping preparation against cancers that primary raw material is prepared from, and is used for the treatment of clinically various malignant tumours, and curative effect is good.
CAULIS MARSDENIAE TENACISSIMAE mainly relies on natural breed and limited wild resource to supply with at present.Due to the biological nature of himself There are few many fruits of flower, plant natural propagation power is low.In addition, high-intensity felling is gathered, and causes the sharply atrophy of current CAULIS MARSDENIAE TENACISSIMAE wild resource amount, manually foster or cultivate imperative.The quick obtaining of high quality seedling is that CAULIS MARSDENIAE TENACISSIMAE is cultivated the most key basic link.Planting seed can be bred seedling; but plant need be after limit growth more all the year round can solid seed; and There are few many fruits of flower ripening rate is low; therefore; explore a kind of CAULIS MARSDENIAE TENACISSIMAE high quality seedling asexual reproduction method quick, proper scale that has, significant to guaranteeing the lasting supply of CAULIS MARSDENIAE TENACISSIMAE raw medicinal material.
At present, the research of CAULIS MARSDENIAE TENACISSIMAE mainly concentrates on effective ingredients in plant and pharmacological action aspect thereof, there is no the research report of the asexual Technique on Cuttage Propagation of CAULIS MARSDENIAE TENACISSIMAE.CAULIS MARSDENIAE TENACISSIMAE is mainly distributed in the middle low altitude area that economize in Yunnan, Guangxi, Guizhou etc., and distributed areas are narrower, and plant rattan is loose porous, include a large amount of milk, after gathering, evaporation water loss is fast, thereby plant cuttage propagation method routinely, cutting is easily rotted, and is difficult to Rapid Rooting or rooting rate extremely low.
Summary of the invention
The present invention seeks to solve CAULIS MARSDENIAE TENACISSIMAE cuttage vegetative propagation and be difficult to survive, the technical problem that rooting rate is low, provide a kind of suitability for scale production, breeding fast, be easy to take root, the cuttage breeding method of CAULIS MARSDENIAE TENACISSIMAE that survival rate is high.
The cuttage breeding method of a kind of CAULIS MARSDENIAE TENACISSIMAE provided by the present invention, comprises the following steps:
(1) the bar time of adopting of cuttage branch and the selection of cuttage branch
In the lignified CAULIS MARSDENIAE TENACISSIMAE rattan of taking the annual 3-5 month to grow more than 3 years, it is cuttage branch;
(2) foundation in seedbed:
In the smooth red-yellow soil area of the karst landform physical features of height above sea level 800m～1000m, build the plastic tunnel of high 2m～4m, at plastic tunnel, built-in 10cm is high, the seedbed of wide 2m, the matrix that for seedbed, peat, perlite, river sand mix for 1:1:1 ratio by volume, stromal thickness is 15cm, it is 60%～80% sunshade net that light transmittance is built in seedbed top, matrix replaces sterilization once with the carbendazim of 500 times of liquid of dilution and the potassium permanganate of 500 times of liquid of dilution, waters permeable standby;
(3) pretreatment of cuttage branch
The cuttage branch that step (1) is gathered is cut into containing two joints, the cutting that length is 15cm～20cm, and cutting base portion is cut inclined-plane at 45 °, cutting base portion is dipped in soon after 20 seconds in 500 times of liquid carbendazim solutions, then the IBA solution that is placed in 300mg/L soaks 2 hours;
(4) cuttage
Behind the described seedbed of the cutting inserting step (2) that will process through step (3), water permeablely, the seeding row spacing of cutting is 8cm * 8cm;
(5) after cuttage, manage
After cuttage first week, controlling soil humidity was 90%～95%, and day temperature is controlled at 25 ℃～34 ℃, and nocturnal temperature is controlled at 20 ℃～24 ℃; It is 80%～89% that second week is controlled soil humidity, and day temperature is controlled at 30～35 ℃, and nocturnal temperature is controlled at 25～28 ℃, and since the 3rd week, controlling soil humidity was 60%～70%, and diurnal temperature maintains 20 ℃～35 ℃; During whole cuttage, every mornings 8 point～8 ventilate 30 minutes thirty, noons 13, point～14 ventilated 1 hour, until remove sunshade net after rooting of cuttings, after hardening, can transplant.
The invention has the beneficial effects as follows:
1, the inventive method is by the suitable comprehensive technical measure of key link, make the rooting rate of CAULIS MARSDENIAE TENACISSIMAE cutting reach 86%～93%, the seedling stalwartness of taking root, transplanting survival rate reaches more than 92%, solved because CAULIS MARSDENIAE TENACISSIMAE plant rattan is loose porous, included a large amount of milk, cutting is easily rotted, cutting survival is difficult, the problem that rooting rate is low; Solved the difficulty that the solid cycle of CAULIS MARSDENIAE TENACISSIMAE is long, There are few many fruits of flower ripening rate is low and be difficult to carry out seminal propagation, for CAULIS MARSDENIAE TENACISSIMAE artificial planting seedling breeding provides new approach.
2, operation is simple and feasible, cost is low, the cycle is short for the inventive method; cuttage only needs 3 months to seedling; realized CAULIS MARSDENIAE TENACISSIMAE vegetative propagation fast and effectively; reached suitability for scale production, breeding fast, be easy to the object of taking root, survival rate is high, to the popularization of the rattan choiceness that speeds passenger flow with guarantee that the continual and steady supply of anticancer bulk drug has very important function and significance.
3, the inventive method can also make offspring keep the merit of medicinal material, expands numerous high-quality strain, shortens the breed breeding cycle, for CAULIS MARSDENIAE TENACISSIMAE artificial cultivation provides high quality seedling material.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated.
Embodiment 1
Test site: Honghe state, Yunnan Province Mengzi city fascine dam.
(1) the bar time of adopting of cuttage branch and the selection of cuttage branch
Taking the lignified CAULIS MARSDENIAE TENACISSIMAE rattan of growing 3 years in March, 2013 is cuttage branch;
(2) foundation in seedbed:
At the Yunnan Province of height above sea level 800m Mengzi city fascine dam, choose the plastic tunnel of building high 2m on the smooth red soil of physical features, at the built-in high 10cm of plastic tunnel, the seedbed of wide 2m, the matrix that for seedbed, peat, perlite, river sand mix for 1:1:1 ratio by volume, stromal thickness is 15cm, it is 60% sunshade net that light transmittance is built in seedbed top, matrix is alternately sterilized once with the potassium permanganate of 500 times of liquid of 50% carbendazol wettable powder and 500 times of liquid of dilution, waters permeable standby;
(3) pretreatment of cuttage branch
Above-mentioned cuttage branch is cut into containing two joints, length is the cutting of 16cm left and right, cutting base portion is cut inclined-plane at 45 °, cuttings base portion is dipped in soon after 20 seconds in 500 times of liquid of 50% carbendazol wettable powder, then IBA (indolebutyric acid) solution that is placed in 300mg/L soaks 2 hours;
(4) cuttage
With the waddy close with cutting diameter, on seedbed, by seeding row spacing 8cm * 8cm, beat the hole that 5cm is dark, by the cutting of handling well the compacting that inserts in the hole, water permeable.
(5) after cuttage, manage
After cuttage first week, controlling soil humidity was 90%～95%, and day temperature is controlled at 25 ℃～27 ℃, and nocturnal temperature is controlled at 22 ℃～24 ℃; It is 80%～84% that second week is controlled soil humidity, and day temperature is controlled at 30 ℃～32 ℃, and nocturnal temperature is controlled at 25 ℃～28 ℃, and since the 3rd week, controlling soil humidity was 60%～70%, and diurnal temperature maintains 20 ℃～35 ℃; During whole cuttage, every mornings 8 point～8 ventilate half an hour thirty, noons 13, point～14 ventilated one hour, until rooting of cuttings, then hardening can be transplanted in the usual way.
Embodiment 2
Test site: copper work, Honghe state, Yunnan Province Jin Ping county.
(1) the bar time of adopting of cuttage branch and the selection of cuttage branch
Taking the lignified CAULIS MARSDENIAE TENACISSIMAE rattan of growing 4 years in May, 2012 is cuttage branch;
(2) foundation in seedbed:
In copper work, height above sea level 1000m Jin Ping county, choose red-yellow soil level land, the foot of the hill, build the plastic tunnel of high 4m, at plastic tunnel, built-in 10cm is high, the seedbed of wide 2m, the matrix that for seedbed, peat, perlite, river sand mix for 1:1:1 ratio by volume, stromal thickness is 15cm, it is 80% sunshade net that light transmittance is built in seedbed top, matrix is alternately sterilized once with the potassium permanganate of 500 times of liquid of 50% carbendazol wettable powder and 500 times of liquid of dilution, waters permeable standby;
(3) pretreatment of cuttage branch
The cuttage branch that step (1) is gathered is cut into containing two joints, length is the cutting of 15-20cm, cutting base portion is cut inclined-plane at 45 °, cuttings base portion is dipped in soon after 20 seconds in 500 times of liquid of 50% carbendazol wettable powder, then IBA (indolebutyric acid) solution that is placed in 300mg/L soaks 2 hours;
(4) cuttage
Behind the described seedbed of the cutting inserting step (2) that will process through step (3), water permeablely, the line space of cutting is 8cm * 8cm;
(5) after cuttage, manage
After cuttage first week, controlling soil humidity was 90%～95%, and day temperature is controlled at 28 ℃～34 ℃, and nocturnal temperature is controlled at 22 ℃～24 ℃; It is 85%～89% that second week is controlled soil humidity, and day temperature is controlled at 33 ℃～35 ℃, and nocturnal temperature is controlled at 25～28 ℃, and since the 3rd week, controlling soil humidity was 60%～70%, and diurnal temperature maintains 25 ℃～35 ℃; During whole cuttage, every mornings 8 point～8 ventilate half an hour thirty, noons 13, point～14 ventilated one hour, until rooting of cuttings, then hardening can be transplanted in the usual way.
The result of the test of embodiment 1 and embodiment 2 refers to table 1.
The test effect of table 1 embodiment 1-2
CAULIS MARSDENIAE TENACISSIMAE wild resource is seriously damaged, and medicinal material demand is still soaring year by year, and medicinal material artificial planting is extremely urgent.High quality seedling breeding is the basis of artificial planting.Limit for length of solid seed demand year of CAULIS MARSDENIAE TENACISSIMAE plant, ripening rate is low, has limited the sapling multiplication of seed.For vegetative propagation, because CAULIS MARSDENIAE TENACISSIMAE plant rattan is loose porous, include a large amount of milk, after gathering, evaporation water loss is fast, plant cuttage breeding routinely, cutting is easily rotted, be difficult to Rapid Rooting, rooting rate is extremely low, be difficult to survive, the present invention is by lot of experiments and research, find Different Altitude, different soils weather, different substrates, homophyletic age not, different cuttings position, different plant growth regulator types and concentration and processing time have a significant impact CAULIS MARSDENIAE TENACISSIMAE rooting rate, technical solution of the present invention is preferred technical scheme on the basis by aforementioned each parameter lot of experiments, the CAULIS MARSDENIAE TENACISSIMAE seedling that it breeds is healthy and strong, rooting rate, survival rate is best, mean elements 12, the long 11.5cm of average root, cutting survival rate is more than 85%, cuttage can be transplanted after 3 months, survival rate is more than 92%.
Claims (1)
1. a method for CAULIS MARSDENIAE TENACISSIMAE cottage propagation, comprises the following steps:
(1) the bar time of adopting of cuttage branch and the selection of cuttage branch
In the lignified CAULIS MARSDENIAE TENACISSIMAE rattan of taking the annual 3-5 month to grow more than 3 years, it is cuttage branch;
(2) foundation in seedbed:
In the smooth red-yellow soil area of the karst landform physical features of height above sea level 800m～1000m, build the plastic tunnel of high 2m～4m, at plastic tunnel, built-in 10cm is high, the seedbed of wide 2m, the matrix that for seedbed, peat, perlite, river sand mix for 1:1:1 ratio by volume, stromal thickness is 15cm, it is 60%～80% sunshade net that light transmittance is built in seedbed top, matrix replaces sterilization once with the carbendazim of 500 times of liquid of dilution and the potassium permanganate of 500 times of liquid of dilution, waters permeable standby;
(3) pretreatment of cuttage branch
The cuttage branch that step (1) is gathered is cut into containing two joints, the cutting that length is 15cm～20cm, and cutting base portion is cut inclined-plane at 45 °, cutting base portion is dipped in soon after 20 seconds in 500 times of liquid carbendazim solutions, then the IBA solution that is placed in 300mg/L soaks 2 hours;
(4) cuttage
Behind the described seedbed of the cutting inserting step (2) that will process through step (3), water permeablely, the seeding row spacing of cutting is 8cm * 8cm;
(5) after cuttage, manage
After cuttage first week, controlling soil humidity was 90%～95%, and day temperature is controlled at 25 ℃～34 ℃, and nocturnal temperature is controlled at 20 ℃～24 ℃; It is 80%～89% that second week is controlled soil humidity, and day temperature is controlled at 30 ℃～35 ℃, and nocturnal temperature is controlled at 25 ℃～28 ℃, and since the 3rd week, controlling soil humidity was 60%～70%, and diurnal temperature maintains 20 ℃～35 ℃; During whole cuttage, every mornings 8 point～8 ventilate 30 minutes thirty, noons 13, point～14 ventilated 1 hour, until remove sunshade net after rooting of cuttings, after hardening, can transplant.
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Cited By (14)
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| CN104145639A (en) * | 2014-07-17 | 2014-11-19 | 广西大学 | Calotropis plant cuttage breeding method |
| CN104170655A (en) * | 2014-07-17 | 2014-12-03 | 广西大学 | Calotropis gigantea grafting propagation method |
| CN104542302A (en) * | 2015-01-28 | 2015-04-29 | 云南农业大学 | Rapid marsdenia tenacissima propagation method |
| CN104542303A (en) * | 2015-01-28 | 2015-04-29 | 云南农业大学 | Rapid marsdenia tenacissima propagation culture media |
| CN104719151A (en) * | 2015-02-27 | 2015-06-24 | 中国医学科学院药用植物研究所云南分所 | Rapid propagation method of dregea sinensis hemsl |
| CN105612990A (en) * | 2015-12-29 | 2016-06-01 | 安徽有机良庄农业科技发展有限公司 | Solar greenhouse green plantation method for cherry tomatoes |
| CN106034602A (en) * | 2016-05-30 | 2016-10-26 | 江苏农牧科技职业学院 | Artificial seedling growing technology for Hypericum japonicum |
| CN107278599A (en) * | 2017-08-03 | 2017-10-24 | 云南信通植物药业有限公司 | A kind of CAULIS MARSDENIAE TENACISSIMAE implantation methods |
| CN107333550A (en) * | 2017-08-03 | 2017-11-10 | 云南信通植物药业有限公司 | A kind of CAULIS MARSDENIAE TENACISSIMAE seed seedling-raising method |
| CN107667795A (en) * | 2017-11-15 | 2018-02-09 | 云南农业大学 | A kind of method for shortening CAULIS MARSDENIAE TENACISSIMAE and spending age and improving flowering rate |
| CN108934811A (en) * | 2018-07-10 | 2018-12-07 | 云南农业大学 | A kind of natural cultivation method of CAULIS MARSDENIAE TENACISSIMAE |
| CN109121980A (en) * | 2018-09-14 | 2019-01-04 | 云南信通植物药业有限公司 | The implantation methods of CAULIS MARSDENIAE TENACISSIMAE |
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| CN112997832A (en) * | 2021-03-04 | 2021-06-22 | 云南海达新生态环境建设有限公司 | Rapid seedling hardening method for rhododendron lapponicum tissue culture seedlings |
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| CN104170655B (en) * | 2014-07-17 | 2016-07-06 | 广西大学 | A kind of Calotropis gigantea propagation by grafiting method |
| CN104170655A (en) * | 2014-07-17 | 2014-12-03 | 广西大学 | Calotropis gigantea grafting propagation method |
| CN104145639A (en) * | 2014-07-17 | 2014-11-19 | 广西大学 | Calotropis plant cuttage breeding method |
| CN104145639B (en) * | 2014-07-17 | 2016-08-24 | 广西大学 | A kind of Calotropis plant cuttage propagation method |
| CN104542302A (en) * | 2015-01-28 | 2015-04-29 | 云南农业大学 | Rapid marsdenia tenacissima propagation method |
| CN104542303A (en) * | 2015-01-28 | 2015-04-29 | 云南农业大学 | Rapid marsdenia tenacissima propagation culture media |
| CN104542303B (en) * | 2015-01-28 | 2016-05-18 | 云南农业大学 | One group of CAULIS MARSDENIAE TENACISSIMAE fast propagating culture medium |
| CN104719151A (en) * | 2015-02-27 | 2015-06-24 | 中国医学科学院药用植物研究所云南分所 | Rapid propagation method of dregea sinensis hemsl |
| CN104719151B (en) * | 2015-02-27 | 2018-03-02 | 中国医学科学院药用植物研究所云南分所 | The rapid propagation method that a kind of Dai Nationality hundred solves |
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| CN106034602A (en) * | 2016-05-30 | 2016-10-26 | 江苏农牧科技职业学院 | Artificial seedling growing technology for Hypericum japonicum |
| CN107278599A (en) * | 2017-08-03 | 2017-10-24 | 云南信通植物药业有限公司 | A kind of CAULIS MARSDENIAE TENACISSIMAE implantation methods |
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| CN107667795A (en) * | 2017-11-15 | 2018-02-09 | 云南农业大学 | A kind of method for shortening CAULIS MARSDENIAE TENACISSIMAE and spending age and improving flowering rate |
| CN108934811A (en) * | 2018-07-10 | 2018-12-07 | 云南农业大学 | A kind of natural cultivation method of CAULIS MARSDENIAE TENACISSIMAE |
| CN108934811B (en) * | 2018-07-10 | 2020-12-15 | 云南农业大学 | Wild tending method for marsdenia tenacissima |
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