CN106857148A - A kind of method that secondary HORMONE TREATMENT promotes the loyal China fir cuttage root-taking seedling of training - Google Patents
A kind of method that secondary HORMONE TREATMENT promotes the loyal China fir cuttage root-taking seedling of training Download PDFInfo
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- CN106857148A CN106857148A CN201710098483.7A CN201710098483A CN106857148A CN 106857148 A CN106857148 A CN 106857148A CN 201710098483 A CN201710098483 A CN 201710098483A CN 106857148 A CN106857148 A CN 106857148A
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- root
- cuttage
- cuttings
- hormone
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0299—Handling or transporting of soil blocks or seedlings
Abstract
A kind of method of seedling of being taken root the invention provides secondary loyal China fir epicormic branch cuttage of HORMONE TREATMENT promotion training,To the loyal China fir spray cuttings root induction hormone kind of training,Concentration and process time,Extraneous root hormone kind,Concentration and process time,And cuttage environmental condition has carried out strict control,Prepared by fringe bar,Root induction HORMONE TREATMENT,Cuttage,Managed after inserting,Cut root treatment,Extraneous root HORMONE TREATMENT,The step such as management is realized after secondary-plug,Due to strengthening to the loyal China fir epicormic branch cuttage root induction of training,Cut the control of root and extraneous root key link,HORMONE TREATMENT have adjusted cuttings inside hormone ratio twice,Ensure that cuttings can be with Rapid Rooting and extraneous root,Improve cuttage seeding root system quality,Epicormic branch cuttage rooting rate is set to have reached more than 90%,Cuttings of taking root planting percent reaches more than 90% level,To realize that preferable technical foundation has been established in the loyal China fir cultivating seedlings industrialized development of training.
Description
Technical field
The present invention relates to achievements in forest-tree seedling breeding field, and in particular to a kind of secondary HORMONE TREATMENT promotes the loyal China fir cuttage root-taking of training
The method of seedling.
Background technology
The loyal China fir (× Taxodiomeria peizhongii) of training is the late forest genetics expert Ye Peizhong of Nanjing Forestry University
Taught in 1963 using Taxodium mucronatum (Taxodium mucronatum Ten.) (♀) and Chinese cryptomeria
(Cryptomeria fortunei Hooibrenk ex Otto et Dietr.)The remote edge for being hybridized and being obtained is miscellaneous
Hand over and plant, can be rated as " the liger beast " of plant kingdom.The loyal China fir of training is half evergreen species, and tree crown is cylinder or oval, and fast-growing character is obvious
Parent and other taxodiaceae plants better than identical land occupation condition, show the leaf fall period it is short (month of In Nanjing 2 fall leaves, sprouted at the beginning of 3 months
Raw young leaves), tree-like graceful, water-fast wet, Salt And Alkali Tolerance, the good characteristic such as stronger to air-filtering ability.
The loyal China fir of training is distant hybridses, shaky, it is impossible to Seedling propagation to be carried out, based on cutting propagation；But loyal China fir inserts for training
Fringe is taken root difficulty, and adventitious root negligible amounts are given birth in cuttage, and transplanting is difficult to survive, and this bottleneck problem is not yet solved completely so far
Certainly so that the annual a large amount of cuttings of cuttage are only capable of obtaining a small amount of nursery stock.
Nanjing Forestry University carries out systematicness, continues to contents such as the loyal China fir germ plasm resource of training, clonal verification, reproduction techniques
Property scientific research, study emphasis reproduction technique exploitation, successively carried out multiple batches of cottage nursing experiment for many years.Practice card for many years
The real loyal China fir seedling propagation of training is extremely difficult, obtains high quality seedling and realizes that Industrialization of seeds and seedlings is extremely difficult.
By repeatedly trial, and experimental technique is gradually improved, the loyal China fir softwood cutting propagation technology of training makes a breakthrough, and obtains
Success.Promote the loyal China fir cuttage root-taking seedling of training using secondary HORMONE TREATMENT, to spray cuttings root induction hormone kind, concentration and
Process time, extraneous root hormone kind, concentration and process time, and cuttage environmental condition has carried out strict control, by fringe
Bar preparation, root induction HORMONE TREATMENT, cuttage, insert after manage, cut management etc. after root treatment, extraneous root HORMONE TREATMENT, secondary-plug and walk
It is rapid to realize, due to strengthening the control to the root induction of training loyal China fir epicormic branch cuttage, Qie Gen and extraneous root key link, hormone twice
Treatment have adjusted cuttings inside hormone ratio, it is ensured that cuttings can improve cuttage seeding root system quality with Rapid Rooting and extraneous root,
Epicormic branch cuttage rooting rate is set to have reached the level that more than 90%, cuttings of taking root planting percent reaches more than 90%, to realize the loyal China fir of training
Preferable technical foundation has been established in cultivating seedlings industrialized development.
The content of the invention
The invention provides a kind of method that secondary HORMONE TREATMENT promotes the loyal China fir cuttage root-taking seedling of training, spray cuttings is lured
Root-promoting hormone species, concentration and process time are led, extraneous root hormone kind, concentration and process time, and cuttage environmental condition are entered
Go strict control, achieve splendid effect.
The present invention is realized by following technical solution：
A kind of method that secondary HORMONE TREATMENT promotes the loyal China fir cuttage root-taking seedling of training, specific steps include：
(1) prepared by fringe bar：The branch that raw cuttage seeding gave birth to semi-lignified then with 1~2 year is material making cuttings；Fringe bar is adopted
It is late June-early July to integrate preparation time, moisturizing of being sprayed water after fringe bar collection；13~15cm of cutting length, fringe bar rugosity 4mm with
On, top retains 3~4 compound leaves, and upper lower cut requirement is smooth.
(2) root induction HORMONE TREATMENT：Before cuttage, 500~1000mg/L root induction hormone solution (indolebutyric acids are used
IBA and methyl α-naphthyl acetate NAA equal quality ratios mix) soak at 2~3cm of fringe bar base portion, soak time 10~15 seconds.
(3) cuttage：Using perlite as cutting medium, matrix uses 50% carbendazim soluble powder in 7 days before cuttage
500 times of liquid carry out spraying disinfection；Cutting density is 5cm × 10cm, and cuttage depth is the 1/2~2/3 of cuttings length.
(4) managed after inserting：After cuttage, according to weather condition, cuttage bed surface is carried out using automatic or manual spraying apparatus
Spraying, is degree to keep blade face to moisten；Every 7 days matrix was sprayed using the liquid of 50% carbendazol wettable powder 600~800
Sterilized with fringe bar；Fine day, 9:00~16:00 is sheltered from heat or light using the sunshade net of shading rate 50~60%.
(5) root treatment is cut：50~60 days after cuttage, by the cuttings taken root by gently being taken out in matrix, cut using sharp
Knife carries out cutting back to adventitious root, and it is 5cm or so to retain length.
(6) extraneous root HORMONE TREATMENT：Soaked using 500~1000mg/L extraneous root hormone (methyl α-naphthyl acetate NAA) solution after cutting root
At 3~5cm of adventitious root base portion, soak time 10~15 seconds.
(7) managed after secondary-plug：Cuttings of taking root after by extraneous root HORMONE TREATMENT is gently imbedded in pearlite interstitial substance again, strain
Line-spacing 5cm × 10cm；Timely and appropriate discovery is sprayed, and is degree to keep blade face to moisten；It is soluble multiple using 0.1~0.3% every 10 days
Hefei solution carries out 1 foliage spray to cuttage seeding, topdresses 2~3 times.
30~40 days after cuttage, adventitious root is produced by the induction of cuttings skin zone；50~60 days, rooting percent up to 90% with
On；Cuttage pond can be gone out with acclimatization and transplantses within 90~100 days, cuttings of taking root planting percent is up to more than 90%.
The advantage of technical solution of the present invention is：
(1) use 1~2 year raw nursery stock to give birth to semi-lignified branch then as fringe material processed, and be prepared into dimension
Cuttings, it is desirable to 13~15cm of cutting length, more than fringe bar rugosity 4mm so that cuttage fringe bar possesses physiological age advantage, in cuttings
Portion's nutrient reserve can meet requirement of taking root.
(2) cuttings of HORMONE TREATMENT induction twice can take root and can be with many fibrous roots of hyperplasia.Root induction HORMONE TREATMENT (Yin
Diindyl butyric acid+methyl α-naphthyl acetate) what is solved is the difficult problem of the loyal China fir spray cuttage rooting of training, induction extraneous root HORMONE TREATMENT (methyl α-naphthyl acetate) solution
Certainly be that cuttings is given birth to adventitious root negligible amounts and transplants the problem that survives of being difficult.
(3) root-cutting shovel and extraneous root HORMONE TREATMENT be combined with each other application, raising cuttage seeding root system quality and transplanting survival rate.
Epicormic branch cuttage gives birth to adventitious root negligible amounts, and (i.e. restricted root is long-living to abolish adventitious root apical growth advantage using root-cutting shovel
It is long), using extraneous root HORMONE TREATMENT, promote adventitious root it is mitogenetic go out a large amount of fibrous roots, fibrous root quality is the key that nursery stock transplanting is survived, can
With water balance and nutrient absorption in nursery stock body after maintaining cuttage seeding hardening and transplanting.
Specific embodiment
The present invention is further described using following examples now, but the involved scope of invention is not restricted to
Following examples.
Embodiment 1
A kind of method that secondary HORMONE TREATMENT promotes the loyal China fir cuttage root-taking seedling of training, specific steps include：
1st, cutting bed construction
Automated spray cuttage pond is built, using electronic timing relay, spraying gap can automatically control with spray time；
Cuttage pond side and bottom discharge are unobstructed, it is to avoid draining is difficult to when water supply in media is excessive；Cutting medium uses perlite, and filling is thick
Degree 35cm, perlite carries out spraying disinfection in 7 days before cuttage using 50% carbendazim soluble powder, 500 times of liquid；On cuttage pond
At square 2m, moveable sunshade net (shading rate 50~60%) is set up.
2nd, collection branch, preparation fringe bar
Late June~early July, 1~2 year raw cuttage seeding of clip gives birth to the branch making cuttings of semi-lignified, collection then
Branch and making spray water moisturizing during cuttings；Cuttings manufacturing specification is：13~15cm of length, more than rugosity 4mm, top retains
3 or 4 compound leaves, upper lower cut requirement are smooth.
3rd, root induction HORMONE TREATMENT
Before cuttage, use 1000mg/L root inductions hormone (indolebutyric acid IBA and methyl α-naphthyl acetate NAA equal quality ratios mix)
At solution immersion cuttings base portion 3cm, soak time 15 seconds.
4th, cuttage
Cutting density is 5cm × 10cm, and cuttage depth is the 1/2~2/3 of cuttings length.
5th, managed after inserting
Water management：After cuttage, according to weather condition, cuttage bed surface is sprayed using automatic spraying system, sprayed
Time and interval are respectively 5~10S and 2h, to keep blade face to moisten.
Periodically sterilization：After cuttage, matrix and fringe were sprayed using the liquid of 50% carbendazol wettable powder 600~800 every 7 days
Bar carries out disinfection.
Shelter from heat or light：Fine day, 9:00-16:00 is sheltered from heat or light using the sunshade net of light transmittance 50~60%.
6th, root treatment is cut
50~60 days after cuttage, by the cuttings taken root by gently being taken out in matrix, adventitious root is carried out using sharp scissors
Cutting back, it is 5cm or so to retain length.
7th, extraneous root HORMONE TREATMENT
Soaked using 1000mg/L methyl α-naphthyl acetate NAA solution and cut at the 3~5cm of adventitious root base portion after root, soak time 10 seconds.
8th, managed after secondary-plug
Cuttings imbeds matrix：Cuttings of taking root after by extraneous root HORMONE TREATMENT is gently imbedded in pearlite interstitial substance again, plant
Away from 5cm × 10cm；
Water management：Timely and appropriate discovery is sprayed, and is degree to keep blade face to moisten；
Periodically sterilization：Spraying matrix and fringe bar using the liquid of 50% carbendazol wettable powder 600~800 every 7 days is carried out
Sterilization；
Foliage spray：Foliage spray was carried out to cuttage seeding using 0.1% soluble composite fertilizer's solution every 10 days, is topdressed
3 times.
9th, hardening
30~40 days after secondary-plug, can be by cuttage transplantation of seedlings hardening.Hardening matrix selects yellow soil: turfy soil: pearl
Rock=2: 3: 1, it is sufficiently mixed.After transplanting, root water is poured, watered in right amount according to soil humidity later.Transplant early stage, sun
Slightly shelter from heat or light when light is stronger, can be completely dispensed with after Survival rate of nursery stock and sheltered from heat or light.
Claims (1)
1. a kind of method that secondary HORMONE TREATMENT promotes the loyal China fir cuttage root-taking seedling of training, it is characterised in that use following steps：
(1) prepared by fringe bar：The branch that raw cuttage seeding gave birth to semi-lignified then with 1~2 year is material making cuttings；Fringe bar collection system
The standby time is late June-early July, moisturizing of being sprayed water after fringe bar collection；13~15cm of cutting length, more than fringe bar rugosity 4mm,
Top retains 3~4 compound leaves, and upper lower cut requirement is smooth.
(2) root induction HORMONE TREATMENT：Before cuttage, 500~1000mg/L root inductions hormone solution (indolebutyric acid IBA is used
Mix with methyl α-naphthyl acetate NAA equal quality ratios) soak at 2~3cm of fringe bar base portion, soak time 10~15 seconds.
(3) cuttage：Using perlite as cutting medium, matrix uses 500 times of 50% carbendazim soluble powder in 7 days before cuttage
Liquid carries out spraying disinfection；Cutting density is 5cm × 10cm, and cuttage depth is the 1/2~2/3 of cuttings length.
(4) managed after inserting：After cuttage, according to weather condition, cuttage bed surface is sprayed using automatic or manual spraying apparatus,
It is degree to keep blade face to moisten；Every 7 days matrix and fringe were sprayed using the liquid of 50% carbendazol wettable powder 600~800
Bar is sterilized；Fine day, 9:00~16:00 is sheltered from heat or light using the sunshade net of shading rate 50~60%.
(5) root treatment is cut：50~60 days after cuttage, by the cuttings taken root by gently being taken out in matrix, sharp scissors pair is used
Adventitious root carries out cutting back, and it is 5cm or so to retain length.
(6) extraneous root HORMONE TREATMENT：Soak indefinite after cutting root using 500~1000mg/L extraneous root hormone (methyl α-naphthyl acetate NAA) solution
At 3~5cm of foundation portion, soak time 10~15 seconds.
(7) managed after secondary-plug：Cuttings of taking root after by extraneous root HORMONE TREATMENT is gently imbedded in pearlite interstitial substance again, seeding row spacing
5cm×10cm；Timely and appropriate discovery is sprayed, and is degree to keep blade face to moisten；0.1~0.3% soluble composite fertilizer was used every 10 days
Solution carries out 1 foliage spray to cuttage seeding, topdresses 2~3 times.
30~40 days after cuttage, adventitious root is produced by the induction of cuttings skin zone；50~60 days, rooting percent was up to more than 90%；
Cuttage pond can be gone out with acclimatization and transplantses within 90~100 days, cuttings of taking root planting percent is up to more than 90%.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107568045A (en) * | 2017-08-14 | 2018-01-12 | 中国科学院华南植物园 | A kind of rapid propagation method of emerald Pueraria lobota |
| CN108307911A (en) * | 2018-03-08 | 2018-07-24 | 广州恒盛园林股份有限公司 | A kind of root cutting growth method of barna |
| CN115462248A (en) * | 2021-06-11 | 2022-12-13 | 上海市崇明区港沿镇农业综合技术推广服务中心(上海市崇明区港沿镇畜牧兽医站) | Method for obtaining boxwood container seedlings through substrate cuttage |
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| CN102138482A (en) * | 2011-02-28 | 2011-08-03 | 中国科学院武汉植物园 | Method for carrying out asexual cuttage propagation on vernicia fordii |
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| CN101695249A (en) * | 2009-10-26 | 2010-04-21 | 重庆市中药研究院 | Non-tube rapid propagation method for dendrobium devonianum |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107568045A (en) * | 2017-08-14 | 2018-01-12 | 中国科学院华南植物园 | A kind of rapid propagation method of emerald Pueraria lobota |
| CN108307911A (en) * | 2018-03-08 | 2018-07-24 | 广州恒盛园林股份有限公司 | A kind of root cutting growth method of barna |
| CN115462248A (en) * | 2021-06-11 | 2022-12-13 | 上海市崇明区港沿镇农业综合技术推广服务中心(上海市崇明区港沿镇畜牧兽医站) | Method for obtaining boxwood container seedlings through substrate cuttage |
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