CN108307911A - A kind of root cutting growth method of barna - Google Patents
A kind of root cutting growth method of barna Download PDFInfo
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- CN108307911A CN108307911A CN201810191848.5A CN201810191848A CN108307911A CN 108307911 A CN108307911 A CN 108307911A CN 201810191848 A CN201810191848 A CN 201810191848A CN 108307911 A CN108307911 A CN 108307911A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
The present invention provides a kind of root cutting growth methods of barna, include the following steps：(1) according to the rich seed selection root of rudiment ability and kind root origin；(2) interception and disinfection of root are planted；(3) root slotting illumination, moisture, the control of temperature and substrate composition；(4) type of HORMONE TREATMENT, concentration and time；(5) cuttage mode and insert after water management；(6) divide root and seedling.The present invention chooses the root segment kind root slotting as root of barna root cap marginal belt fibrous root, does not influence that elite stand is crown, easy to operate, breeding coefficient is big；Optimize the proportioning for the slotting matrix of kind rough degree, cuttage mode, root inserted suitable for root, the time of hormone kind, hormone concentration and HORMONE TREATMENT; shorten bud sprout time; effectively increase bud germination rate, rooting rate and planting percent; the fast seedling growing of barna in a short time is realized, is laid a good foundation for the development of resources and protection of barna.
Description
Technical field
The present invention relates to a kind of method for culturing seedlings of barna, more particularly to a kind of root cutting growth method of barna.
Background technology
Barna (CratevareligiosaG.Forster) is Capparidaceae (Capparidaceae) barna category
(Crateva) perennial defoliation small arbor is distributed in the areas such as Taiwan, Guangdong, Guangxi and the Sichuan in China, is a kind of excellent
Flower trees of one's native land.Its timber can make musical instrument and small timber, and material is light and hard, its wood of the ground fisherman such as Taiwan and Ryukyu
Material is carved into small fish shape and makees bait, and toxic to fish, therefore has the title of " barna "；From barna bark extract plumage China fir alcohol have it is anti-inflammatory,
Antibacterial, antitumor, diuresis, blood pressure lowering and the effect for reducing cholesterol level, pericarp have certain antifungic action；Fruit contains
Alkaloid, can make adhesive, and pericarp can make dyestuff.It can be seen that barna is a kind of excellent ornamental, medicinal, industrial seeds,
With wide development prospect.However, under natural conditions, the setting percentage of barna and the germination rate of seed are very low, and breeding is very
It is difficult.Barna mainly uses overhead layering to breed at present, has no the report that stem is inserted or root is slotting.The propagation method of overhead layering operates
It is complex, and tree management to elite stand and it is crown destroy it is very big.Application No. is the Chinese inventions of CN201710133857.4
It discloses " a kind of culture medium box set of barna Vitro Quick Reproduction ", the culture medium of the barna Vitro Quick Reproduction described in the invention
Box set provides a new method for barna fast seedling growing.Studies have shown that with 1 year or more for the breeding cycle, the in vitro training of plant
Support it is very fast than other propagation methods, but since to establish sterile system early period more time-consuming for cultured in vitro, with other modess of reproduction
It compares, the reproduction speed in 1 year is not dominant.We have found that the stem of barna is inserted into work in the Primary Study of early period
Rate is low, and each segment can only cultivate 1 young plant, and root is inserted and is easy to happen adventitious bud, and survival rate is high, and each segment can generate very much
Bud, breeding potential are high, are ideal propagating materials, are expected to improve barna breeding side's relatively low problem of speed in a short time in vitro.No
Only in this way, the root segment suitable for rugosity has no effect on the crown and vigor of elite stand, more horn of plenty is inserted in source compared with stem, breeding operation compared with from
Body culture is easy, will lay the foundation for the resources development and utilization of barna and protection.
Invention content
The technical problem to be solved in the present invention is to provide a kind of root cutting growth method of barna, can significantly improve rooting rate,
Planting percent, and seedling is fast, breeding coefficient is big.
In order to solve the above technical problems, the technical scheme is that：
A kind of root cutting growth method of barna, includes the following steps：
(1) seed selection root：Late Febuary-late March takes the root segment with fibrous root as kind at the root cap edge of barna elite stand
Root deposits in shady place；
(2) interception and disinfection of root are planted：The kind root that step (1) is adopted back is truncated into the slotting section of 15cm long, rejecting is undermined band
The slotting section of pest and disease damage is put into for use after disinfectant solution impregnates after tying up；
(3) root slotting illumination, moisture, the control of temperature and substrate composition：Seedbed be arranged in greenhouse, seedbed it is upper
Side's installation automatic sprayer, the matrix of different ratio is respectively placed in different seedbeds, is also equipped in seedbed temperature automatically controlled
The temperature control of device, matrix is 23-25 DEG C；
(4) HORMONE TREATMENT：Will through step (2) treated insert section be put into plant hormone solution impregnate after it is to be inserted；
(5) cuttage mode and insert after water management：By step (4), treated inserts segment cuttage and enter in seedbed, inserts section
Top is equal with the surface of matrix, and every 2d auto sprayings 1 time spray 1 fungicide per 7d；
(6) divide root and seedling：24-38d after inserting, adventitious bud are grown successively；60d divides root hardening, according to a of bud when dividing root
Number and position, intercept in the Seedling bag that corresponding root segment is transplanted in greenhouse, using sand, perlite, peat mixture as matrix；
Each spraying is primary sooner or later daily, and Seedling bag is removed greenhouse by new fibrous root after growing, and carries out common aqueous management until seedling.
Further, in step (1) of the present invention, the rugosity of root segment is 0.3-0.6cm.
Further, in step (2) of the present invention, the item number that section is inserted when tying up is 30, and disinfectant solution is a concentration of
The liquor potassic permanganate of 0.05-0.3%, soaking time 10-60min.
Further, in step (3) of the present invention, matrix includes sand, perlite, peat, and the volume ratio of three is (0-
4):(0-4):(0-4)；The obscurity of greenhouse is 20%, and the length in seedbed is 5m, width 1.2m.
Further, sand of the present invention, perlite, peat volume ratio be 1:1:2.
Further, matrix of the present invention further includes shell powder, sand, perlite, peat, shell powder volume ratio be 1:
1:2:0.5, the preparation process of the shell powder is：
Clam shell coarse powder one is obtained after clam shell is crushed, clam shell coarse powder one is added in distilled water, 2h is impregnated at 80 DEG C, is taken out
After filter, will obtained filter residue crush after obtain clam shell coarse powder two, by clam shell coarse powder two be added hydrogen peroxide in, be placed in ultrasonic wave hair
It is ultrasonically treated 15min under 250W power in raw device, is filtered after taking-up, obtained filter residue is washed with deionized 3 times, after drying
Grinding crosses 40 mesh and sieves to obtain shell powder.
Further, in step (4) of the present invention, a concentration of 200-1000mg/L of plant hormone solution, when immersion
Between be 10-60min, plant hormone IBA, NAA, GA3Or rat-tail algae extract, the preparation process of the rat-tail algae extract
For：
Sargassum thunbergii is cleaned, smash to pieces after with tissue refiner be homogenized 8min, be transferred in distilled water and extract 100min at 40 DEG C,
Leaching liquor and particle are obtained after centrifugation, and particle, cellulase are added in distilled water, 200min is digested at 35 DEG C, it is cooling
It is centrifugally separating to obtain supernatant after to room temperature, concentrated by rotary evaporation is in liquid is thick after mixing with leaching liquor by supernatant
Only obtain rat-tail algae extract.
Further, in step (5) of the present invention, the mode of cuttage be it is flat bury, oblique cutting or straight cutting, spacing in the rows when cuttage
For 20cm × 10cm, spray time is each 30min, and the temperature in seedbed controls within the scope of 23-25 DEG C, and fungicide is carbendazim
1000ppm liquids, fungicide spray 2min every time.
Further, in step (6) of the present invention, sand in the matrix of hardening, perlite, peat volume ratio be 1:1:
2, a diameter of 12cm of Seedling bag are highly 15cm, and the time of spraying is 60s, and the temperature in seedbed is controlled in 23-25 DEG C of range
It is interior.
Compared with prior art, the invention has the advantages that：
1) barna root is easy to happen adventitious bud, can grow up to the new plant for keeping elite stand characteristic, be ideal cutting propagation
Material, the present invention choose the fibrous root kind root slotting as root at root cap edge, do not influence elite stand is crown, easy to operate, rooting rate is high,
Seedling is fast, breeding coefficient is big.
2) present invention optimizes cuttage mode, the proportioning of the slotting matrix of root, hormone kind, hormone concentration and HORMONE TREATMENTs
Time shortens bud sprout time, and effectively increases bud germination rate, rooting rate and planting percent, realizes quickly educating for barna
Seedling provides technical support for the industrial seedling rearing of barna, lays a good foundation for protection of resources, the development and utilization of barna.
3) clam shell is the casing part of freshwater mussel, contains more calcium and other a small amount of minerals.The present invention is by it through powder
It is broken, impregnate, be ultrasonically treated after obtain the shell powder with porous structure, be added in matrix calcareous etc. in addition to that can supplement offer
Except minerals, also there is preferable water-retaining property and water penetration, be conducive to further promote taking root for barna seedling.
4) sargassum thunbergii, scientific name Sargassumthunbergii (Mert.) Kuntze, the Endogenous hormones containing there are many, this
Invention pass it through homogenate, extraction, enzymolysis and etc. Endogenous hormones are effectively extracted to obtain rat-tail algae extract, can
As IBA, NAA, GA3Another Plant Hormone in addition is conducive to that the bud of barna seedling is further promoted to sprout.
Specific implementation mode
Below in conjunction with specific embodiment, the present invention will be described in detail, herein illustrative embodiments and their description of the invention
For explaining the present invention, but it is not as a limitation of the invention.English initialism of the present invention is explained as follows：IBA is
Indolebutyric acid, NAA are methyl α-naphthyl acetate, GA3It is gibberellin.
Term：
Term " hardening " is tamed, and can improve root seedlings plugging to the adaptability of external environmental condition, it is photosynthetic to improve its
Ability promotes root seedlings plugging healthy and strong, is finally reached the purpose for improving root seedlings plugging transplanting survival rate.
Embodiment 1
The root cutting growth method of barna, includes the following steps：
(1) seed selection root：Late Febuary-late March takes the band fibrous root that rugosity is 0.3cm at the root cap edge of barna elite stand
Root segment as kind of a root, deposit in shady place；
(2) interception and disinfection of root are planted：The kind root that step (1) is adopted back is truncated into the slotting section of 15cm long, rejecting is undermined band
The slotting section of pest and disease damage is put into after 0.1% liquor potassic permanganate immersion 30min for use after tying up；
(3) root slotting illumination, moisture, the control of temperature and substrate composition：Seedbed is arranged in the greenhouse that obscurity is 20%
In greenhouse, the length in seedbed is 5m, and the top of width 1.2m, seedbed install automatic sprayer, the matrix prepared are placed in
In seedbed, sand in matrix, perlite, peat, shell powder volume ratio be 1:1:2:0.5, temperature automatically controlled dress is also equipped in seedbed
It sets, the temperature control of matrix is 23-25 DEG C.Wherein, the preparation process of shell powder is：Clam shell coarse powder is obtained after clam shell is crushed
One, clam shell coarse powder one is added in distilled water, 2h is impregnated at 80 DEG C, is filtered after taking-up, freshwater mussel is obtained after obtained filter residue is crushed
Clam shell coarse powder two is added in hydrogen peroxide, is placed in supersonic generator and is ultrasonically treated 15min under 250W power by shell coarse powder two,
It is filtered after taking-up, obtained filter residue is washed with deionized 3 times, is ground after drying, crossed 40 mesh and sieve to obtain shell powder；
(4) HORMONE TREATMENT：It will be to be inserted after impregnating 30min in step (2) the IBA solution that treated inserts section 500mg/L；
(5) cuttage mode and insert after water management：By step (4) treated insert section by spacing in the rows 20cm × 10cm, tiltedly
It is inserted into seedbed, the top for inserting section is equal with the surface of matrix, automatic sprayer is sprayed 1 time per 2d, spray time is
The temperature of 30min, seedbed control within the scope of 23-25 DEG C, spray 1 carbendazim 1000ppm liquid after inserting per 7d, spray every time
2min；
(6) divide root and seedling：24-38d after inserting, adventitious bud are grown successively；60d divides root hardening, according to a of bud when dividing root
Number with position distribute corresponding root system be transplanted to a diameter of 12cm in greenhouse, highly for 15cm Seedling bag in, be with volume ratio
1:1:2 sand, perlite, peat mixture be matrix；The same step of temperature (5) in seedbed, stop-spraying carbendazim liquid are early daily
Late each spraying is primary, each 60s, and Seedling bag is removed greenhouse by new fibrous root after growing, and carries out common aqueous management until seedling.
Embodiment 2
As different from Example 1, in step (1), the rugosity for planting root is 0.4cm.
Embodiment 3
As different from Example 1, in step (1), the rugosity for planting root is 0.5cm.
Embodiment 4
As different from Example 1, in step (1), the rugosity for planting root is 0.6cm.
Embodiment 5
As different from Example 3, in step (3), sand in matrix, perlite, peat volume ratio be 4:0:0.
Embodiment 6
As different from Example 3, in step (3), sand in matrix, perlite, peat volume ratio be 0:4:0.
Embodiment 7
As different from Example 3, in step (3), sand in matrix, perlite, peat volume ratio be 0:0:4.
Embodiment 8
As different from Example 3, in step (3), sand in matrix, perlite, peat volume ratio be 2:1:1.
Embodiment 9
As different from Example 3, in step (3), sand in matrix, perlite, peat volume ratio be 1:2:1.
Embodiment 10
As different from Example 3, in step (3), sand in matrix, perlite, peat volume ratio be 1:1:2.
Embodiment 11
As different from Example 3, in step (4), a concentration of 200mg/L of IBA solution, soaking time 30min.
Embodiment 12
As different from Example 3, in step (4), a concentration of 1000mg/L of IBA solution, soaking time 60min.
Embodiment 13
As different from Example 3, in step (4), the hormone of immersion is NAA, a concentration of 200mg/L of NAA solution, leaching
The bubble time is 30min.
Embodiment 14
As different from Example 3, in step (4), the hormone of immersion is NAA, a concentration of 500mg/L of NAA solution, leaching
The bubble time is 60min.
Embodiment 15
As different from Example 3, in step (4), the hormone of immersion is NAA, a concentration of 1000mg/L of NAA solution,
Soaking time is 10min.
Embodiment 16
As different from Example 3, in step (4), the hormone of immersion is GA3, GA3A concentration of 200mg/L of solution, leaching
The bubble time is 10min.
Embodiment 17
As different from Example 3, in step (4), the hormone of immersion is GA3, GA3A concentration of 500mg/L of solution, leaching
The bubble time is 30min.
Embodiment 18
As different from Example 3, in step (4), the hormone of immersion is GA3, GA3A concentration of 1000mg/L of solution, leaching
The bubble time is 60min.
Embodiment 19
As different from Example 3, in step (4), the hormone of immersion is rat-tail algae extract, sargassum thunbergii extract solution
A concentration of 500mg/L, soaking time 30min.Wherein, the preparation process of rat-tail algae extract is：Sargassum thunbergii is cleaned, is smash
After broken with tissue refiner be homogenized 8min, be transferred in distilled water and extract 100min at 40 DEG C, obtained after centrifugation leaching liquor and
Particle, cellulase are added in distilled water, digest 200min at 35 DEG C, be centrifugally separating to obtain after being cooled to room temperature by particle
Clear liquid, by supernatant, concentrated by rotary evaporation obtains rat-tail algae extract until thick in liquid after mixing with leaching liquor.
Embodiment 20
As different from Example 3, in step (5), cuttage mode is flat buries.
Embodiment 21
As different from Example 3, in step (5), cuttage mode is straight cutting.
Comparative example
In order to protrude the effect of barna root cutting growth, the present invention with barna hardwood cutting and seedling raising as a comparison case：Barna
Hardwood cutting and seedling raising method selects 1 year raw branch as cuttings as different from Example 3 in step (1), step (6) is moved
The step of cultivation is：It is transplanted to after seedling grows 3-4 sprouting in a diameter of 12cm, highly in the Seedling bag for being 15cm, and with body
Product is than being 1:1:2 sand, perlite, peat mixture be matrix.
Experimental example：
The seedling growth test of barna in embodiment 1-21 and comparative example is observed and recorded as a result, embodiment 1-21 statistic procedures (5)
Germination rate, average height of seedling, rooting rate, average root long, lateral root number and the middle left sides transplanting 30d of step (6) of middle cuttage 60d or so
Right planting percent；In comparative example statistic procedure (5) in the germination rate of cuttage 90d or so, rooting rate, lateral root number and step (6)
Transplant the planting percent of 30d or so.Statistical result is shown in Table 1：
Table 1
From the point of view of the embodiment 3 of table 1 and the result of comparative example, barna root cutting growth 34d just starts rudiment, and height of seedling reaches
15.4cm, rooting rate is up to 98%, and lateral root number is up to 18.5, planting percent 98%, and hardwood cutting and seedling raising 76d just starts rudiment, sprouts
Bud rate is only 54%, and lateral root number is also only 5.7, and planting percent is only 48%, illustrates that root cutting growth can greatly shorten the seedling time, is increased
Plus side radical improves rooting rate and planting percent.
In terms of kind rough degree, it can be seen that from embodiment 1-4：Bud germination rate, average height of seedling, rooting rate, lateral root number and seedling
Rate is improved as kind of rough degree (0.3-0.6cm) increases.But from the point of view of the source of kind of root, the smaller quantity of kind rough degree is got over
It is more and smaller to the injury of elite stand；The bigger quantity of rugosity is fewer, also bigger to the injury of elite stand.In summary factor,
0.5cm is that barna root inserts kind of the best size of rough degree.
In terms of cutting medium, the effect of the embodiment 5-7 of husky, perlite or peat soil as matrix when is used alone not
The effect of the embodiment 8-10 of such as 3 kinds of matrix mixing, and with sand in mixed-matrix：Perlite：Peat soil volume ratio is 1:1:2
(embodiment 6) is best, and adventitious bud is sprouted after cuttage 33d, germination rate 93%, height of seedling 15.7cm, and rooting rate is up to 97%, side
Radical is up to 18.3, and planting percent is up to 98%.It can be seen that from the result of embodiment 3 and 10：The matrix (embodiment 17) of shell powder is added
Rooting rate, lateral root number, the planting percent of obtained barna are slightly better than embodiment 6, other aspects are slightly worse than embodiment 6, illustrate freshwater mussel
Shell powder is conducive to further hestening rooting.
In terms of growth hormone, it can be seen that from the result of embodiment 3 and 9-17：IBA, which may advantageously facilitate, takes root, GA3Be conducive to
Bud is promoted to sprout, the effect of NAA is in IBA and GA3Between, planting percent and lateral root number are substantially directly proportional.From embodiment 3 and 18
As a result it can be seen that：It is slightly better than using rat-tail algae extract as the germination rate of plant hormone (embodiment 18) obtained barna real
Example 3 is applied, other aspects illustrate that rat-tail algae extract is conducive to that bud is further promoted to sprout then slightly poorer to embodiment 3.
In terms of cuttage mode, oblique cutting (embodiment 3) and flat bury between (embodiment 20), straight cutting (embodiment 21) have significance difference
It is different, and the flat difference buried between straight cutting is little.The growth and development of tree root and the aeration status of soil and water content are close
Correlation, it is flat to bury that root system is relatively shallower, although aeration status is preferable, the easy dehydration of cuttings influences survival rate；And straight cutting earthing mistake
Deep, cuttings poor aeration also affects the development of root system, and only oblique cutting can meet the aeration condition of cuttings, also make cuttings not
The excessive dehydration of meeting, therefore oblique cutting survival rate highest.
The above-described embodiments merely illustrate the principles and effects of the present invention, and is not intended to limit the present invention.It is any ripe
The personage for knowing this technology can all carry out modifications and changes to above-described embodiment without violating the spirit and scope of the present invention.Cause
This, institute is complete without departing from the spirit and technical ideas disclosed in the present invention by those of ordinary skill in the art such as
At all equivalent modifications or change, should by the present invention claim be covered.
Claims (9)
1. a kind of root cutting growth method of barna, it is characterised in that：Include the following steps：
(1) seed selection root：Late Febuary-late March takes the root segment with fibrous root as kind of a root at the root cap edge of barna elite stand, deposits
It is put in shady place；
(2) interception and disinfection of root are planted：The kind root that step (1) is adopted back is truncated into the slotting section of 15cm long, rejecting is undermined worm in spite of illness
Harmful slotting section is put into for use after disinfectant solution impregnates after tying up；
(3) root slotting illumination, moisture, the control of temperature and substrate composition：Seedbed is arranged in greenhouse, and the top in seedbed is pacified
Automatic sprayer is filled, the matrix of different ratio is respectively placed in different seedbeds, automatic temp controller is also equipped in seedbed,
The temperature control of matrix is 23-25 DEG C；
(4) HORMONE TREATMENT：Will through step (2) treated insert section be put into plant hormone solution impregnate after it is to be inserted；
(5) cuttage mode and insert after water management：By step (4) treated insert segment cuttage enter in seedbed, insert section top
Equal with the surface of matrix, every 2d auto sprayings 1 time spray 1 fungicide per 7d；
(6) divide root and seedling：24-38d after inserting, adventitious bud are grown successively；60d divides root hardening, when dividing root according to the number of bud with
Position intercepts in the Seedling bag that corresponding root segment is transplanted in greenhouse, using sand, perlite, peat mixture as matrix；Daily
Sooner or later each spraying is primary, and Seedling bag is removed greenhouse by new fibrous root after growing, and carries out common aqueous management until seedling.
2. a kind of root cutting growth method of barna according to claim 1, it is characterised in that：In the step (1), root segment
Rugosity be 0.3-0.6cm.
3. a kind of root cutting growth method of barna according to claim 1, it is characterised in that：In the step (2), tie up
When insert section item number be 30, disinfectant solution be a concentration of 0.05-0.3% liquor potassic permanganate, soaking time 10-
60min。
4. a kind of root cutting growth method of barna according to claim 1, it is characterised in that：In the step (3), matrix
Including sand, perlite, peat, the volume ratio of three is (0-4):(0-4):(0-4)；The obscurity of greenhouse is 20%, seedling
The length of bed is 5m, width 1.2m.
5. a kind of root cutting growth method of barna according to claim 4, it is characterised in that：The sand, perlite, peat
Volume ratio be 1:1:2.
6. a kind of root cutting growth method of barna according to claim 4, it is characterised in that：The matrix further includes clam shell
Powder, sand, perlite, peat, shell powder volume ratio be 1:1:2:0.5, the preparation process of the shell powder is：
Clam shell coarse powder one is obtained after clam shell is crushed, and clam shell coarse powder one is added in distilled water, 2h, mistake after taking-up are impregnated at 80 DEG C
Filter obtains clam shell coarse powder two after crushing obtained filter residue, and clam shell coarse powder two is added in hydrogen peroxide, supersonic generator is placed in
It is ultrasonically treated 15min under middle 250W power, is filtered after taking-up, obtained filter residue is washed with deionized 3 times, is ground after drying
Mill crosses 40 mesh and sieves to obtain shell powder.
7. a kind of root cutting growth method of barna according to claim 1, it is characterised in that：In the step (4), plant
A concentration of 200-1000mg/L of hormone solution, soaking time 10-60min, plant hormone IBA, NAA, GA3Or sargassum thunbergii
The preparation process of extract, the rat-tail algae extract is：
Sargassum thunbergii is cleaned, smash to pieces after with tissue refiner be homogenized 8min, be transferred in distilled water and extract 100min at 40 DEG C, centrifuge
Leaching liquor and particle are obtained after separation, and particle, cellulase are added in distilled water, 200min is digested at 35 DEG C, is cooled to room
Supernatant is centrifugally separating to obtain after temperature, concentrated by rotary evaporation obtains until thick in liquid after mixing with leaching liquor by supernatant
To rat-tail algae extract.
8. a kind of root cutting growth method of barna according to claim 1, it is characterised in that：In the step (5), cuttage
Mode be it is flat bury, oblique cutting or straight cutting, spacing in the rows when cuttage is 20cm × 10cm, and spray time is each 30min, the temperature in seedbed
Within the scope of 23-25 DEG C, fungicide is carbendazim 1000ppm liquids for degree control, and fungicide sprays 2min every time.
9. a kind of root cutting growth method of barna according to claim 1, it is characterised in that：In the step (6), hardening
Matrix in sand, perlite, peat volume ratio be 1:1:2, a diameter of 12cm of Seedling bag are highly 15cm, spraying when
Between be 60s, the temperature in seedbed controls within the scope of 23-25 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201810191848.5A CN108307911B (en) | 2018-03-08 | 2018-03-08 | Root cutting seedling raising method for fish wood |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810191848.5A CN108307911B (en) | 2018-03-08 | 2018-03-08 | Root cutting seedling raising method for fish wood |
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| CN108307911A true CN108307911A (en) | 2018-07-24 |
| CN108307911B CN108307911B (en) | 2022-04-19 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109716941A (en) * | 2019-02-27 | 2019-05-07 | 四川农业大学 | A kind of Peony Root segment cuttage quick-breeding method |
| CN111264200A (en) * | 2020-03-19 | 2020-06-12 | 四川七彩林科股份有限公司 | Wisteria root cutting propagation method |
| WO2020220672A1 (en) * | 2019-04-28 | 2020-11-05 | 广东美景环境科技有限公司 | Clustered cultivation method for tabebuia chrysantha |
| CN115399185A (en) * | 2022-09-29 | 2022-11-29 | 潮州市香林沉香种植科技有限公司 | Method for promoting quick incense formation of Kyara |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101874447A (en) * | 2009-11-17 | 2010-11-03 | 金陵科技学院 | Beach plum root cutting seeding method |
| CN104255484A (en) * | 2014-09-12 | 2015-01-07 | 南京通泽农业科技有限公司 | Rapid propagation method of crateva unilocularis |
| CN105340678A (en) * | 2015-12-05 | 2016-02-24 | 安徽省农业科学院园艺研究所 | Clonal propagation method of Chinese walnuts |
| CN106431631A (en) * | 2016-08-31 | 2017-02-22 | 高亚莉 | Growth promotion watermelon fertilizer and preparation method thereof |
| CN106857148A (en) * | 2017-02-22 | 2017-06-20 | 南京林业大学 | A kind of method that secondary HORMONE TREATMENT promotes the loyal China fir cuttage root-taking seedling of training |
-
2018
- 2018-03-08 CN CN201810191848.5A patent/CN108307911B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101874447A (en) * | 2009-11-17 | 2010-11-03 | 金陵科技学院 | Beach plum root cutting seeding method |
| CN104255484A (en) * | 2014-09-12 | 2015-01-07 | 南京通泽农业科技有限公司 | Rapid propagation method of crateva unilocularis |
| CN105340678A (en) * | 2015-12-05 | 2016-02-24 | 安徽省农业科学院园艺研究所 | Clonal propagation method of Chinese walnuts |
| CN106431631A (en) * | 2016-08-31 | 2017-02-22 | 高亚莉 | Growth promotion watermelon fertilizer and preparation method thereof |
| CN106857148A (en) * | 2017-02-22 | 2017-06-20 | 南京林业大学 | A kind of method that secondary HORMONE TREATMENT promotes the loyal China fir cuttage root-taking seedling of training |
Non-Patent Citations (3)
| Title |
|---|
| 徐英宝 等: ""广东省城市林业优良树种及栽培技术"", 《广东省城市林业优良树种及栽培技术》 * |
| 杨建兵等: "紫花醉鱼木全光照喷雾扦插育苗技术试验", 《甘肃林业科技》 * |
| 蒋军: ""紫花醉鱼木栽培要点及繁殖方法"", 《现代园艺》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109716941A (en) * | 2019-02-27 | 2019-05-07 | 四川农业大学 | A kind of Peony Root segment cuttage quick-breeding method |
| WO2020220672A1 (en) * | 2019-04-28 | 2020-11-05 | 广东美景环境科技有限公司 | Clustered cultivation method for tabebuia chrysantha |
| CN111264200A (en) * | 2020-03-19 | 2020-06-12 | 四川七彩林科股份有限公司 | Wisteria root cutting propagation method |
| CN115399185A (en) * | 2022-09-29 | 2022-11-29 | 潮州市香林沉香种植科技有限公司 | Method for promoting quick incense formation of Kyara |
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