CN101874447A - Beach plum root cutting seeding method - Google Patents
Beach plum root cutting seeding method Download PDFInfo
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- CN101874447A CN101874447A CN2009102342290A CN200910234229A CN101874447A CN 101874447 A CN101874447 A CN 101874447A CN 2009102342290 A CN2009102342290 A CN 2009102342290A CN 200910234229 A CN200910234229 A CN 200910234229A CN 101874447 A CN101874447 A CN 101874447A
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- 238000000034 method Methods 0.000 title claims abstract description 25
- 235000008433 Prunus maritima var maritima Nutrition 0.000 title claims abstract description 12
- 238000005520 cutting process Methods 0.000 title claims abstract description 10
- 238000010899 nucleation Methods 0.000 title claims abstract description 8
- 240000007322 Prunus maritima Species 0.000 title abstract description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 20
- 241000196324 Embryophyta Species 0.000 claims abstract description 4
- 239000007921 spray Substances 0.000 claims description 18
- 239000004576 sand Substances 0.000 claims description 12
- 235000014347 soups Nutrition 0.000 claims description 12
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 8
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 8
- 239000006013 carbendazim Substances 0.000 claims description 8
- 229910052799 carbon Inorganic materials 0.000 claims description 8
- 238000005507 spraying Methods 0.000 claims description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 6
- 230000018044 dehydration Effects 0.000 claims description 6
- 238000006297 dehydration reaction Methods 0.000 claims description 6
- 238000011081 inoculation Methods 0.000 claims description 6
- 235000015097 nutrients Nutrition 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 238000005286 illumination Methods 0.000 claims description 4
- 239000011159 matrix material Substances 0.000 claims description 4
- 238000012545 processing Methods 0.000 claims description 4
- 235000016709 nutrition Nutrition 0.000 claims description 3
- 230000035764 nutrition Effects 0.000 claims description 3
- 239000002689 soil Substances 0.000 claims description 3
- 241000195940 Bryophyta Species 0.000 claims description 2
- 241001123597 Funneliformis mosseae Species 0.000 claims description 2
- 241000238631 Hexapoda Species 0.000 claims description 2
- 239000005842 Thiophanate-methyl Substances 0.000 claims description 2
- 201000009310 astigmatism Diseases 0.000 claims description 2
- 238000001816 cooling Methods 0.000 claims description 2
- 201000010099 disease Diseases 0.000 claims description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 2
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 claims description 2
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims description 2
- 239000002068 microbial inoculum Substances 0.000 claims description 2
- 208000004141 microcephaly Diseases 0.000 claims description 2
- 239000003415 peat Substances 0.000 claims description 2
- 239000002985 plastic film Substances 0.000 claims description 2
- 229920006255 plastic film Polymers 0.000 claims description 2
- 238000010008 shearing Methods 0.000 claims description 2
- QGHREAKMXXNCOA-UHFFFAOYSA-N thiophanate-methyl Chemical group COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC QGHREAKMXXNCOA-UHFFFAOYSA-N 0.000 claims description 2
- 244000132691 Prunus maritima var. maritima Species 0.000 claims 1
- 230000004083 survival effect Effects 0.000 abstract description 14
- 206010020649 Hyperkeratosis Diseases 0.000 abstract description 7
- 230000008569 process Effects 0.000 abstract description 6
- 235000013399 edible fruits Nutrition 0.000 abstract description 4
- 238000011161 development Methods 0.000 abstract description 2
- 230000003716 rejuvenation Effects 0.000 abstract description 2
- 235000015571 Prunus maritima Nutrition 0.000 description 10
- 238000007726 management method Methods 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 244000144987 brood Species 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000002420 orchard Substances 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
Classifications
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- Y02P60/216—
Landscapes
- Cultivation Of Plants (AREA)
Abstract
The invention belongs to the technical field of vegetative propagation of plants, in particular to a beach plum root cutting seeding method. By applying the invention, the problems of callus decay of a beach plum cuttage shoot root, low rooting rate, low survival rate and tender seeding which is easy to lose water and then withers are solved. Acclimatization in the process strengthens the growth and development of roots, stems and leaves and rejuvenation in the beach plum cuttage seeding process, remarkably improves the survival rate of field planting and establishes the firm foundation for the cultivation and generalization of the beach plum seeding which is a unique forest fruit.
Description
One, technical field
This invention belongs to the plant asexual multiplication technology field, is specifically related to a kind of beach plum root cutting seeding method.
Two, background technology
Shore plum (Prunus maritima) is a kind of desert shrub fruit tree, originates in the U.S., Atlantic bank, from the Newfoundland to North Carolina Na Zhou distribution is arranged all.It can adapt to existence in arid (comprising very low soil moisture content), barren, strong wind, sand, unstable matrix and highly-saline environment, have sweet good to eat fruit, has the characteristics of beautifying the environment and recovering husky living vegetation concurrently.The shore plum will be a pioneer that stands in the breach in the development of beach and salt tolerant orchard, other solonchak zone industry.Domestic successful introduction, but the bottleneck of brood body supply has greatly limited its popularization cultivation in China.Make remarkable progress aspect breeding at cell engineering Seedling with the research team headed by the Nanjing University, but also have problems such as cost is too high, the survival rate of the open-air field planting of tissue cultivating seedling is not high.Therefore carry out the road that propagation in scale is undoubtedly another light by low-cost, cuttage technique easy and simple to handle.But the shore plum is adopted in hard branch or the epicormic branch cuttage process, typical problems such as the root callus rots, rooting rate is low, survival rate is low, the more delicate very easily dehydration of seedling body withers often occur, and process management is very strict, the operation easier height.Therefore, the selection of best plugged ear kind, access time and method, the preparation of suitable matrix, insert the screening of back management and hardening off method, the simplification of the program of growing seedlings and management method, to the raising of the callus healthy growth of shore plum seedling, rooting rate, survival rate, healthy and strong growth of seedling body and seedling-cultivating method are promoted most important, and optimizing and combining of correlative factor is the cuttage technique key of success.
Three, summary of the invention
The problem that the present invention need solve is: overcome the problem that shore plum cuttage shoot root callus rots, rooting rate is low, survival rate is low, the more delicate very easily dehydration of seedling body withers and transplanting survival rate is low.
Concrete technical scheme of the present invention is: the present invention is directed to the problem that callus is perishable in the feature of shore plum and biological property and the cuttage process, rooting rate is low, survival rate is low, the more delicate very easily dehydration of seedling body withers and growing-seedling period is long, invented the beach plum root cutting method of " seed selection root → cut kind of root → vernalization → dosing and processing → seedbed arrangement → cuttage and insert after management → spraying liquid and nutrient solution → root division hardening and transplanting ".Difficult problems such as the root callus rots, rooting rate is low, survival rate is low in the beach plum root cutting process, have been solved, perfect moisture and nutrition (particularly nitrogen, P elements) supply conditions, strengthened growing and rejuvenation of root in the beach plum root cutting process, stem, leaf, significantly improved its survival rate, promoted laying a solid foundation for the cultivation of characteristic woods fruit shore plum seedling.
Concrete technical scheme is as follows:
1. the choose seeds Best Times and the points for attention of root.
2. cut kind of root and respective handling method
3. vernalization and respective handling method
4. the determining of the screening of hormone kind, concentration, optimum organization and processing time
5. slotting machine arrangement
6. cuttage and insert back management (water control, temperature control, light-operated etc.)
7. root division hardening off method
The present invention compared with prior art, its beneficial effect is:
Root insert grow seedlings easy and simple to handle, the reproduction coefficient height, cost is low and can keep parent's proterties.The shore plum is in hard branch or epicormic branch cuttage process, and the normal typical problem that occurs is: cuttage shoot root callus rots, rooting rate is low, survival rate is low, the more delicate very easily dehydration of seedling body withers.Use the present invention to address the above problem effectively, significantly improve survival rate, and the strong stress resistance of seedling.Innovation part of the present invention mainly contains following points: the 1. 2. 3. 4. root division hardening off method of management of the factors such as cuttage Hou Shui, temperature, light of vernalization and respective handling method of the select time of plugged ear kind and the method for cutting kind of root.Use the survival rate of beach plum root cutting seedling of the present invention can reach more than 86%, open-air transplanting survival rate can reach more than 95%, really realizes the asexual fast numerous advantage of shore plum seedling, promotes cultivation for its large tracts of land reliable guarantee is provided.
Four, embodiment
1. the root of choosing seeds: early December-early January, the root cap edge takes the root segment of thick 0.5-1cm band fibrous root and does kind of a root, plants the root collection in a large number and takes longlyer, is not exposed to the sun when long, deposit in shady and cool place;
2. cut kind of a root: the kind root that adopt back in the field is cut slotting section that is cut into long 15cm immediately, and major part is cut into flat mouthful, and microcephaly's angle is rejected and split root, damage and damage by disease and insect part, and slotting section of shearing is a bundle with the 30-50 bar, and the carbendazim soup that immerses 800ppm is handled 20s;
3. vernalization: in the high and dry plot of physical features, dig the ditch of the wide 100cm of dark 80cm, bottom of trench is the clean wet river sand of shop 20cm earlier, and root is erected at husky going up (greatly head-up, upside down little), charge into fine sand between bundle, the husky one deck root segment of one deck, pendulum is extremely apart from exit or entrance of a clitch 15cm place, cover fine sand above, earthing is trained into mound again, and covered with plastic film carries out the winter and hides vernalization;
4. dosing and processing: indolebutyric acid and methyl liquid were mixed in by volume 1: 1, and separately final concentration is 1000ppm in the mixed liquor.Take out kind of root and insert section March, and base portion adopts fast libation at an ancient wedding ceremony method, uses above-mentioned mixed liquor to handle 10s, again with to be inserted behind the active carbon on the otch libation at an ancient wedding ceremony;
5. seedbed arrangement: the seedbed is arranged in the green house, the thick cobblestone of slotting machine bottom shop 10cm, and the thick thin river sand of 30cm is spread on top, and automatic sprayer is installed on the slotting machine;
6. cuttage reaches and inserts the back management: the slotting section of the kind root after soup is handled, slotting section of active carbon is inserted slotting machine immediately on the otch libation at an ancient wedding ceremony, by spacing in the rows 30cm * 30cm, 30 ° of inclinations are gone in the husky bed, the top is equal with Shamian Island, and slotting machine humidity is adjusted into one grade at dusk 30%~40% with automatic sprayer, automatically control water spraying time, water spray was 1 time in three days, had water droplet to ooze out after water spray presses with sand at the duration and was as the criterion, general 60s, with the illumination of sunshade network control system, insert in the 3d of back and see astigmatism, see direct light, shading at noon after 2 weeks gradually, whole day is seen light behind the 30d, the slotting machine temperature is than the high 2-3 of air themperature ℃, and maintains 22-25 ℃, if air themperature can be sprayed when being higher than 25 ℃ or be aeration-cooling, insert every 1 carbendazim 1000ppm soup of 7d spray in back or thiophanate methyl 800ppm soup, hocket, behind the slotting section growth 45d, in water spray, add the spray nutrient solution, see Table 1, nutrient solution and spray carbendazim soup carry out simultaneously, every 7d spray 1 time.
7. root division hardening and transplanting: insert about the about 40d in back, root is inserted seedling and is grown successively, when treating height of seedling 10cm, selects the reasonably strong bud in 1-3 orientation to do trunk; Can the root division hardening after 60 days, corresponding root system is distributed in number and position according to trunk during root division, moves the basin hardening behind the active carbon on the libation at an ancient wedding ceremony of the new wound of root; During the root division hardening, the inoculation commercially available Glomus mosseae (Nicol.﹠amp in Beijing City Agriculture and Forestry Institute; Gerd.) microbial inoculum, hardening matrix are the peat composed of rotten mosses of 2 parts of volumes and the mellow soil mixture of 1 part of volume, every basin inoculation 20g, and the basin of inoculation basin directly is 13cm, the high 13cm of basin; Temperature, illumination condition during hardening are the same, stop-spraying carbendazim soup, and each week spray one time of nutrition liquid sees Table 1, the control water yield is as the criterion with not dehydration of blade, reduces the water yield gradually, and water spray once every night, water spraying time is 60s, can open-air field planting after 90 days, and preceding 1 week of field planting puts the seedling basin in the open.
Table 1 nutrient composition proportioning
Composition | Dosage (g/L) | Composition | Dosage (g/L) | ||
??1 | ??KH2PO 4 | ??0.16 | ??7 | Ironic citrate | ??0.03 |
??2 | ??KNO 3 | ??0.51 | ??8 | ??ZnSO 4·7H 2O | ??0.28 |
??3 | ??Ca(NO 3) 2 | ??0.80 | ??9 | ??CuSO 4·5H 2O | ??0.08 |
??4 | ??MgSO 4 | ??0.49 | ??10 | ??H 2MO 4·H 2O | ??0.04 |
??5 | ??H 3BO 3 | ??2.86 | |||
??6 | ??MnCl 2·4H 2O | ??1.81 |
Claims (1)
1. beach plum root cutting seeding method is characterized in that being made of following steps:
1. the root of choosing seeds: early December-early January, the root cap edge takes the root segment of thick 0.5-1cm band fibrous root and does kind of a root, plants the root collection in a large number and takes longlyer, is not exposed to the sun when long, deposit in shady and cool place;
2. cut kind of a root: the kind root that adopt back in the field is cut slotting section that is cut into long 15cm immediately, and major part is cut into flat mouthful, and microcephaly's angle is rejected and split root, damage and damage by disease and insect part, and slotting section of shearing is a bundle with the 30-50 bar, and the carbendazim soup that immerses 800ppm is handled 20s;
3. vernalization: in the high and dry plot of physical features, dig the ditch of the wide 100cm of dark 80cm, bottom of trench is the clean wet river sand of shop 20cm earlier, root is erected on the sand, greatly head-up, upside down little, charge into fine sand between bundle, the husky one deck root segment of one deck, pendulum covers fine sand, again earthing above to apart from exit or entrance of a clitch 15cm place, train into mound, covered with plastic film carries out the winter and hides vernalization;
4. dosing and processing: indolebutyric acid and methyl liquid were mixed in by volume 1: 1, separately final concentration is 1000ppm in the mixed liquor, and take out kind of root and insert section March, and base portion adopts fast libation at an ancient wedding ceremony method, use above-mentioned mixed liquor to handle 10s, again with to be inserted behind the active carbon on the otch libation at an ancient wedding ceremony;
5. seedbed arrangement: the seedbed is arranged in the green house, the thick cobblestone of slotting machine bottom shop 10cm, and the thick thin river sand of 30cm is spread on top, and automatic sprayer is installed on the slotting machine;
6. cuttage reaches and inserts the back management: the slotting section of the kind root after soup is handled, slotting section of active carbon is inserted slotting machine immediately on the otch libation at an ancient wedding ceremony, by spacing in the rows 30cm * 30cm, 30 ° of inclinations are gone in the husky bed, the top is equal with Shamian Island, slotting machine humidity is adjusted into one grade at dusk 30%~40% with automatic sprayer, controls water spraying time automatically, water spray was 1 time in three days, have water droplet to ooze out after water spray presses with sand at the duration and be as the criterion, general 60s is with the illumination of sunshade network control system, insert in the 3d of back and see astigmatism, see direct light after 2 weeks gradually, the shading at noon, whole day is seen light behind the 30d, the slotting machine temperature is than the high 2-3 of air themperature ℃, and maintain 22-25 ℃, if air themperature can be sprayed when being higher than 25 ℃ or be aeration-cooling, insert every 1 carbendazim 1000ppm soup of 7d spray in back or thiophanate methyl 800ppm soup, hocket, after inserting section growth 45d, in water spray, add the spray nutrient solution, nutrient solution and spray carbendazim soup carry out simultaneously, every 7d spray 1 time;
7. root division hardening and transplanting: insert about the about 40d in back, root is inserted seedling and is grown successively, when treating height of seedling 10cm, selects the reasonably strong bud in 1-3 orientation to do trunk; Can the root division hardening after 60 days, corresponding root system is distributed in number and position according to trunk during root division, moves the basin hardening behind the active carbon on the libation at an ancient wedding ceremony of the new wound of root; During the root division hardening, the inoculation commercially available Glomus mosseae (Nicol.﹠amp in Beijing City Agriculture and Forestry Institute; Gerd.) microbial inoculum, hardening matrix are the peat composed of rotten mosses of 2 parts of volumes and the mellow soil mixture of 1 part of volume, every basin inoculation 20g, and the basin of inoculation basin directly is 13cm, the high 13cm of basin; Temperature, illumination condition during hardening are the same, stop-spraying carbendazim soup, each week spray one time of nutrition liquid, the control water yield, be as the criterion with not dehydration of blade, reduce the water yield gradually, water spray once every night, water spraying time is 60s, can open-air field planting after 90 days, and preceding 1 week of field planting puts the seedling basin in the open.
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CN2009102342290A CN101874447B (en) | 2009-11-17 | 2009-11-17 | Beach plum root cutting seedling method |
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Cited By (13)
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CN102318502A (en) * | 2011-09-26 | 2012-01-18 | 金陵科技学院 | Root grating and seedling raising method of beach plum |
CN102630444A (en) * | 2011-12-10 | 2012-08-15 | 何寒 | Non-test-tube rapid propagation technology of plants |
CN103460944A (en) * | 2013-09-18 | 2013-12-25 | 南京大学连云港高新技术研究院 | Prunus maritima marshall hard branch cutting propagation method |
CN103718908A (en) * | 2014-01-14 | 2014-04-16 | 金陵科技学院 | Method for cultivating seedlings of prunus maritime root suckers |
CN103749137A (en) * | 2014-01-16 | 2014-04-30 | 四川农业大学 | Cutting seedling method for malus toringoides |
CN105230314A (en) * | 2015-11-05 | 2016-01-13 | 广西壮族自治区农业科学院花卉研究所 | Cutting propagation method for jasmine |
CN105532270A (en) * | 2016-01-25 | 2016-05-04 | 金陵科技学院 | Beach plum scion grafting seedling culture method |
CN105900769A (en) * | 2016-04-18 | 2016-08-31 | 旌德县隐龙梅园生态农场 | Red plum cultivation method |
CN106416810A (en) * | 2016-09-30 | 2017-02-22 | 金华市农业科学研究院 | Labor-saving grafting reproduction method capable of increasing emergence rate of grape rootstock cuttings |
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CN105230314B (en) * | 2015-11-05 | 2018-03-13 | 广西壮族自治区农业科学院花卉研究所 | The cuttage breeding method of jasmine |
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CN109315158A (en) * | 2018-09-29 | 2019-02-12 | 十堰中盈生态农业发展有限公司 | Northwest of Hubei Province oil tree peony fast asexual propagation technology |
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CN110603969A (en) * | 2019-10-12 | 2019-12-24 | 金陵科技学院 | Root cutting method for hybrid paper mulberry |
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