CN104303804A - Method for facilitating cutting rooting of tilia miqueliana by magnetic treatment - Google Patents

Abstract

The invention provides a Nanjing linden twig cutting method, which strictly controls the intensity and frequency of magnetized water treatment for twig cuttings, exogenous hormone treatment before cutting, and cutting environmental conditions. Water treatment, cutting, post-planting management steps to achieve, due to the strengthening of the control of the key links of Nanjing Linden twig cuttings, magnetic treatment and exogenous hormones to stimulate the cuttings to form callus and root induction, optimize the rooting environment, ensure It ensures that the cuttings can take root quickly, and at the same time reduces the probability of cuttings rot, so that the survival rate of tender branch cuttings has reached a level of more than 85%, which has laid a good seedling foundation for the realization of the industrialization of Nanjing bass seedling cultivation.

Description

A method of magnetic treatment to promote rooting of Nanjing Tilia cuttings

technical field

The invention relates to the field of tree seedling propagation, in particular to a method for promoting rooting of Nanjing Linden cuttings by magnetic treatment.

Background technique

Nanjing Tilia (Tilia miqueliana Maxim.), also known as Tilia miqueliana Maxim., is commonly known as "linden tree". Nanjing linden has a strong fragrance, thick leaves, and tall and straight trunks. It is a landscaping, medicinal, timber, and nectar plant. Because of its mysterious religious and cultural color, it has been introduced and cultivated in Japan in large numbers. Nanjing Tilia was once widely distributed as a native tree species in East China, but due to difficult reproduction and habitat destruction, its population and individual numbers have gradually declined. Research on reproductive technology has become a top priority for the protection, development and utilization of Nanjing Tilia resources!

Nanjing Tilia's propagation technology is mainly based on seed propagation and cutting propagation, but Nanjing Tilia's low seed yield, strong "small and big years", low seed plumpness and deep dormancy characteristics seriously affect seedling propagation, making cutting propagation the most potential propagation method . At present, there is no Nanjing linden cutting propagation applied to seedling production at home and abroad. The main reason is that the cuttings are difficult to root and the cuttings are easy to rot during the cutting process, which often leads to seedling failure.

Since 2003, Nanjing Forestry University has carried out systematic and continuous scientific research on Nanjing Tilia germplasm resources and reproduction technology, focusing on the development of reproduction technology, and has conducted many researches on Nanjing Tilia seedling propagation technology and cutting seedling raising technology . Years of scientific research has proved that it is extremely difficult to reproduce Nanjing bass seedlings, and it is extremely difficult to obtain high-quality seedlings and realize the industrialization of seedlings.

After many attempts and gradually improving the experimental methods, Nanjing Tilia twig cutting propagation technology has made a breakthrough and achieved success. Magnetic treatment promotes rooting technology of Nanjing Linden twig cuttings, including strict control of intensity and frequency of magnetized water treatment for twig cuttings, exogenous hormone treatment before cutting, and cutting environmental conditions. Treatment, cutting, and post-planting management steps are realized, which strengthens the control of the key links of Nanjing Linden twig cuttings. Magnetic treatment and exogenous hormones work together to stimulate callus formation and root induction of cuttings, optimize the rooting environment, and ensure cuttings It can take root quickly, reduce the probability of cuttings rot, and greatly increase the survival rate of tender branch cuttings, laying a good seedling foundation for the realization of the industrial development of Nanjing bass seedling cultivation.

Contents of the invention

The invention provides a method of magnetic treatment to promote the rooting of Nanjing linden twig cuttings. The intensity and frequency of magnetized water treatment for twig cuttings, the treatment of exogenous hormones before cutting and the environmental conditions of cuttings are strictly controlled, and excellent results have been obtained. Effect.

The present invention is realized through the following technical solutions:

A method for promoting rooting of Nanjing Tilia cuttings by magnetic treatment, the specific steps comprising:

(1) Spike preparation: use the semi-lignified branches of 1-2 year old seedlings as materials to make corresponding specifications of spikes; the collection and preparation time of spikes is in the middle and late August; use 1000mg/L high Potassium manganate solution was eluted for 5 minutes, and then thoroughly washed with water.

The specifications of the spikes are: length 10-15cm, thickness 0.5-0.8cm, 1 1/2-1 leaf is reserved at the top of the spike, the top of the spike is truncated, and the bottom is obliquely truncated.

(2) Hormone treatment: when cutting, the base of the cuttings is dipped with 1000-1500 mg/kg of hormone (indole butyric acid IBA) powder.

(3) Magnetized water treatment: use perlite as the cutting matrix; before cutting, the matrix is fully watered with 7500-10000GS magnetized water; after cutting, the matrix is fully watered with 7500-10000GS magnetized water again. From cutting to seedling hardening and transplanting, an automatic spray system is used to keep the water in the cutting pool, the water source uses 7500-10000GS magnetized water, and the spraying time and interval are 5-10S and 2h, respectively.

(4) Cutting: The cutting density is 5cm×10cm, and the lower part of the cuttings is inserted into the substrate 2/3.

(5) Post-planting management: After cuttings, use 50% carbendazim wettable powder 600-800 solution every 7 days to spray the matrix and cuttings for disinfection; % of shade net shade.

10-15 days after cutting, the cuttings will form callus; 25-30 days will induce a large number of adventitious roots; 50-60 days, the seedlings can be hardened and transplanted out of the cutting pond; the rooting rate of the cuttings of twigs and spikes is over 85%.

The advantage of technical scheme of the present invention is:

(1) Use the semi-lignified branches of the 1-2 year-old seedlings as ear-making materials, and prepare ear bars of corresponding specifications, so that the cutting ear bars have the advantage of physiological age, and the internal nutrient reserves of the cutting ears can meet the rooting requirements.

(2) Using indole butyric acid powder to treat cuttings can promote rooting of cuttings, and at the same time, the action time of powder is longer than that of solvent.

(3) Use magnetized water to treat the cutting bed and spray regularly, which can stimulate the cuttings to form callus and root induction. The magnetized water has special physical characteristics, which has a strong effect of inducing rooting, and optimizes the rooting environment to ensure rapid rooting of cuttings.

(4) Regular spraying and shading with sunshade nets can ensure the water demand during the cutting process of the cuttings, reduce the drying of the cuttings under high temperature, and enable the cuttings to take root in a superior environment.

(5) Using perlite as the cutting substrate, long spraying time interval and short single spraying time can better reduce the moisture of the substrate and reduce the rot of the spikes.

detailed description

The following examples are now used to further illustrate the present invention, but the scope of the invention is not limited to the following examples.

Example 1

A method for promoting rooting of Nanjing Tilia cuttings by magnetic treatment, the specific steps comprising:

1. Collect branches and prepare spikes

In mid-to-late August, in the morning and evening or on cloudy days, choose semi-lignified branches of 1-2 years old seedlings as materials to make cuttings. The cuttings are 10-15cm long and 0.5-0.8cm thick, and one 1/2-1 Leaves, spikes truncate at upper end, obliquely truncate at lower end.

2. Disinfection treatment of spikes

Use 1000mg/L potassium permanganate solution to elute the base 3-5cm of the spikes for 5 minutes, and then wash them thoroughly with clean water.

3. Disinfection of cutting substrate

Perlite is used as the cutting substrate, and the cutting substrate is filled with a thickness of 25-35cm; one day before the perlite cutting, spray with 50% carbendazim wettable powder 600-800 solution and stir thoroughly.

4. Cuttings

Before cutting, the matrix is fully watered with 7500-10000GS magnetized water.

When cutting, the base of the cuttings is dipped with hormone (indole butyric acid IBA) powder 1000-1500mg/kg; the cutting density is 5cm×10cm, and the lower part of the cuttings is inserted into the substrate 2/3.

After cutting, the matrix is fully watered with 7500-10000GS magnetized water again.

5. Management after insertion

Water management: After cutting to hardening and transplanting, an automatic spray system is used to keep the water in the cutting pool, the water source uses 7500-10000GS magnetized water, and the spraying time and interval are 5-10S and 2h respectively.

Periodic disinfection: after cuttings, use 50% carbendazim wettable powder 600-800 solution every 7 days to spray the matrix and cuttings for disinfection.

Shading: On sunny days, use shade nets with a light transmittance of 50-60% from 9:00 to 16:00.

6. Seedling hardening

A large number of adventitious roots are induced 25 to 30 days after cutting, and the seedlings can be hardened and transplanted out of the cutting pond in 50 to 60 days. When transplanting cutting seedlings, the roots can be cut appropriately to promote the development of the root system of the seedlings. The medium for hardening seedlings is yellow heart soil: peat soil: perlite = 2:3:1, fully mixed. After transplanting, pour enough root-fixing water, and then water appropriately according to the humidity of the substrate. In the early stage of transplanting, when the sun is strong, there is a little shade, and after the seedlings survive, there is no need for shade at all.

Claims (1)

1. magnetic treatment promotes a method for tilia miqueliana cuttage root-taking, it is characterized in that, adopts following steps:

(1) fringe bar preparation: give birth to the fringe bar of semi-lignified branch for material making dimension then with 1 ~ 2 year raw nursery stock; It is mid or late August that fringe bar gathers preparation time; Fringe bar base portion 3 ~ 5cm uses 1000mg/L liquor potassic permanganate wash-out 5min, re-uses clear water and thoroughly cleans.

Described fringe bar specification is: long 10 ~ 15cm, thick 0.5 ~ 0.8cm, fringe bar upper end retains 1 1/2 ~ 1 blade, and fringe bar upper end is truncate, lower end bevel.

(2) HORMONE TREATMENT: during cuttage, fringe bar base portion dips hormone (indolebutyric acid IBA) pulvis 1000 ~ 1500mg/kg.

(3) magnetized water treatment: using perlite as cutting medium; Before cuttage, matrix uses 7500 ~ 10000GS magnetized drinking water fully to irrigate; After cuttage, matrix reuses 7500 ~ 10000GS magnetized drinking water and fully irrigates.To in acclimatization and transplants process after cuttage, use automatic spraying system to keep cuttage pond moisture, water source uses 7500 ~ 10000GS magnetized drinking water, and spray time and interval are respectively 5 ~ 10S and 2h.

(4) cuttage: cutting density is 5cm × 10cm, matrix 2/3 is inserted in cuttings bottom.

(5) management after inserting: after cuttage, used 50% carbendazol wettable powder 600 ~ 800 liquid to spray matrix and the sterilization of fringe bar every 7 days; Fine day, 9:00-16:00 uses the sunshade net of light transmittance 50 ~ 60% to shelter from heat or light.

After cuttage 10 ~ 15 days, cuttings formed callus; Induction in 25 ~ 30 days produces a large amount of adventive root; Within 50 ~ 60 days, cuttage pond can be gone out by acclimatization and transplants; Spray tloig-cutlage rooting rate more than 85%.