CN115989756A - A kind of cutting method of medicinal marijuana - Google Patents

Abstract

The invention relates to a cutting method of medicinal marijuana, which relates to the technical field of plant cultivation. The purpose of the invention is to solve the problems of slow rooting speed, low survival rate, long breeding time and high labor cost in the current breeding of medicinal cannabis. Method: Soak the morphological lower end of the pruned branch in the rooting solution diluted 1000 times and composed of 2.5% naphthaleneacetic acid and 2.5% indolebutyric acid for 30s; cool the sterilized matrix and put it into a container for compaction. Dig holes; insert the soaked medicinal marijuana branches into the dug holes and compact them with the substrate, spray with 50% carbendazim diluted 500 times, and place in shade under the conditions of a temperature of 25°C and a humidity of 70% for 3 After 1-2 weeks of shading, the cuttings of medicinal marijuana were completed; during the cultivation period, 50% carbendazim diluted 500 times was sprayed every 3 days. The invention can obtain a cutting method of medicinal marijuana.

Description

A kind of cutting method of medicinal marijuana

technical field

The invention relates to the technical field of plant cultivation, in particular to a cutting method of medicinal cannabis.

Background technique

Industrial hemp is an annual herbaceous plant of the Cannabis family Cannabis genus, dioecious and widely distributed around the world. Industrial hemp is an important economic crop widely used in textile and medicine fields. The female flowers and leaves of industrial hemp are rich in cannabinoids, among which tetrahydrocannabinol (THC) and cannabidiol (CBD) are the main two cannabinoids. Tetrahydrocannabinol (THC) has hallucinogenic activity, while cannabidiol (CBD) is a non-psychoactive component and has a wealth of pharmacological activities. Medicinal cannabis refers to cannabis varieties with high content of cannabidiol (CBD) and tetrahydrocannabinol (THC) content <0.3%, and because cannabidiol (CBD) has important medicinal value, it is currently on the market The high demand for cannabis makes the efficient breeding of cannabis for medicinal use especially important. However, the current breeding of medicinal cannabis has disadvantages such as slow rooting speed, low survival rate, long breeding time and high labor costs.

Contents of the invention

The purpose of the present invention is to provide a cutting method of medicinal cannabis in order to solve the problems of slow rooting speed, low survival rate, long breeding time and high labor cost in the current breeding of medicinal cannabis.

A kind of cutting method of medicinal marijuana, carries out according to the following steps:

Step 1. Select a medicinal marijuana branch with two stem nodes at the top of a branch that is not fully lignified or semi-lignified, cut the morphological lower end of the branch obliquely, and cut the morphological upper end flat, and the branch retains a stem node, remove The length of the branch after the terminal bud is 5-6cm; the morphological lower end of the pruned branch is placed in the rooting solution diluted 1000 times and soaked for 30-60s to obtain the soaked medicinal cannabis branch; the rooting solution diluted 1000 times The massfraction of naphthaleneacetic acid in the liquid is 2.5%, and the massfraction of indolebutyric acid is 2.5%;

Step 2: Add water to the matrix, then sterilize at 121°C for 15-20 minutes, cool the sterilized matrix, put it into a container for compaction, and dig a hole in the center of the matrix;

Step 3. Insert the soaked medicinal marijuana branches in step 1 into the hole dug in step 2 and compact it with the substrate, then spray with 50% carbendazim diluted 500 times, and then spray at a temperature of 25-28°C and humidity Place it in shading for 3 days under the condition of 70-85%, and continue to cultivate for 1-2 weeks after the shading ends, and complete the cuttings of medicinal marijuana; during the cultivation period, spray 50% carbendazim diluted 500 times every 3 days.

Beneficial effects of the present invention:

The invention discloses a cutting method of medicinal marijuana: using semi-lignified shoots of medicinal marijuana as materials, each cutting branch is 5-6 cm long, one stem node is reserved, the young leaves can be kept, and half of the old leaves are cut off. Remove the top buds. The immersion time of branch in the rooting solution of 1000 times liquid (naphthalene acetic acid NAA2.5%, indole butyric acid IBA2.5%) is 30～60s, and NAA can induce to form adventitious root, and IBA can induce the formation of rhizoplasma, for To promote rooting of cuttings, the combination of two auxins can promote root germination. The method utilizes the high-pressure sterilization method, so that the effect of removing the pathogenic bacteria in the soil is better, and the invasion of the cutting branches by the soil-borne pathogenic bacteria is avoided. In addition, use 500 times liquid 50% carbendazim to spray the cutting seedlings themselves and their environment, including the cover of the plug or the inside of the shed, as well as the cutting seedlings and the substrate, so as to avoid the contact between the branch wounds and the environmental germs, so it can greatly improve the quality of the cuttings. The survival rate of branches. Shade for three days after the cuttings are completed, and open the shed every morning for 1-2 hours to keep the branches active. Through the above method, an efficient medicinal cannabis cutting system is established, and the occupied area is small, which is suitable for a small amount of cutting propagation for scientific research purposes. According to the statistics of the test results, the rooting speed of the expanded cutting branches of the present invention is fast, and it can be observed that there are roots growing out of the lower end of the morphology in about one week, and a large number of roots are observed growing out in about two weeks, with a length of about 1 cm or more. 100%. The invention can effectively carry out cutting propagation of medicinal cannabis, has fast rooting speed and high survival rate, and the survival rate is as high as 100%. The seedling raising cycle is significantly shortened, and the land occupation is small, the transplanting is convenient, and the time and labor costs are greatly reduced.

The invention can obtain a cutting method of medicinal marijuana.

Description of drawings

Fig. 1 is the root system diagram that grows after two weeks of cuttings of Chinese medicinal cannabis branch in embodiment 1;

Fig. 2 is the form of the medicinal marijuana branch two weeks after cutting in embodiment 1.

Detailed ways

Specific embodiment one: the present embodiment a kind of cutting method of medicinal marijuana, carries out according to the following steps:

Step 1. Select a medicinal marijuana branch with two stem nodes at the top of a branch that is not fully lignified or semi-lignified, cut the morphological lower end of the branch obliquely, and cut the morphological upper end flat, and the branch retains a stem node, remove The length of the branch after the terminal bud is 5-6cm; the morphological lower end of the pruned branch is placed in the rooting solution diluted 1000 times and soaked for 30-60s to obtain the soaked medicinal cannabis branch; the rooting solution diluted 1000 times The massfraction of naphthaleneacetic acid in the liquid is 2.5%, and the massfraction of indolebutyric acid is 2.5%;

Step 2: Add water to the matrix, then sterilize at 121°C for 15-20 minutes, cool the sterilized matrix, put it into a container for compaction, and dig a hole in the center of the matrix;

Step 3. Insert the soaked medicinal marijuana branches in step 1 into the hole dug in step 2 and compact it with the substrate, then spray with 50% carbendazim diluted 500 times, and then spray at a temperature of 25-28°C and humidity Place it in shading for 3 days under the condition of 70-85%, and continue to cultivate for 1-2 weeks after the shading ends, and complete the cuttings of medicinal marijuana; during the cultivation period, spray 50% carbendazim diluted 500 times every 3 days.

Specific embodiment two: the difference between this embodiment and specific embodiment one is: in step one, the scissors after the lower end of branch morphology is sterilized with alcohol cuts the 45 ° oblique mouth, and the upper end of the morphology cuts the flat mouth.

Other steps are the same as in the first embodiment.

Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: when pruning in step one, the young leaves of the medicinal marijuana branches are kept, and half of the old leaves are cut off.

Other steps are the same as those in Embodiment 1 or 2.

Embodiment 4: This embodiment differs from Embodiment 1 to Embodiment 3 in that the matrix in step 2 is composed of charcoal, vermiculite, perlite and water.

Other steps are the same as those in Embodiments 1 to 3.

Embodiment 5: This embodiment differs from Embodiments 1 to 4 in that the mass ratio of carbon soil, vermiculite and perlite is 3:1:1.

Other steps are the same as those in Embodiments 1 to 4.

Embodiment 6: This embodiment differs from Embodiment 1 to Embodiment 5 in that the container used in step 2 is a plug tray.

Other steps are the same as those in Embodiments 1 to 5.

Embodiment 7: The difference between this embodiment and Embodiments 1 to 6 is that in step 2, water is added to the matrix, and then placed in an autoclave, and sterilized at 121°C for 15-15°C. 20min.

Other steps are the same as those in Embodiments 1 to 6.

Embodiment 8: This embodiment differs from Embodiment 1 to Embodiment 7 in that the depth of the hole dug in step 2 is 3-5 cm.

Other steps are the same as those in Embodiments 1 to 7.

Embodiment 9: The difference between this embodiment and Embodiment 1 to Embodiment 8 is that in step 3, the depth of inserting the soaked medicinal marijuana branch into the hole is 3-5 cm.

Other steps are the same as those in Embodiments 1 to 8.

Embodiment 10: This embodiment differs from Embodiment 1 to Embodiment 9 in that: in step 3, ventilation is performed for 1-2 hours every morning during the cultivation period, and clear water is sprayed on the leaves every morning and evening.

Other steps are the same as those in Embodiments 1 to 9.

Adopt the following examples to verify the beneficial effects of the present invention:

Embodiment 1: a kind of cutting method of medicinal marijuana, carries out according to the following steps:

Step 1. Select a medicinal marijuana branch with two stem nodes at the top of a branch that is not fully lignified or semi-lignified, cut the morphological lower end of the branch with alcohol-sterilized scissors to cut a 45° oblique cut, and cut the morphological upper end to a flat cut, And the branches keep a stem node, keep the young leaves, cut off half of the old leaves, and the length of the branches after removing the terminal buds is 5-6cm; soak the morphological lower end of the pruned branches in the rooting solution diluted 1000 times for 30 seconds, and get soaked medicinal marijuana sprigs;

The mass fraction of naphthaleneacetic acid in the rooting solution diluted 1000 times is 2.5%, and the mass fraction of indolebutyric acid is 2.5%.

Step 2. The substrate is prepared according to the ratio of peat soil, vermiculite and perlite 3:1:1, adding appropriate amount of water, and the humidity of the substrate is based on the standard of water seepage when holding hands, forming a ball when loosening hands, and dispersing when it falls on the ground; then put it in an autoclave and sterilized at 121°C for 20 minutes, put the sterilized base into a clean tray for compaction after cooling, and poke a hole in the center of the base slightly larger than the diameter of the medicinal marijuana branch. 3cm deep.

Step 3. Insert the soaked medicinal marijuana branch in step 1 vertically into the hole dug in step 2 for 3cm and compact it with the substrate. Cover the tray with a tray cover or build a small shed, and then use 50% diluted 500 times Carbendazim is evenly sprayed on the cover of the hole tray or the inside of the shed, as well as the branches and substrates of medicinal cannabis, and then placed in a shade at a temperature of 25°C and a humidity of 70%; during the cultivation period, open the hole every morning Ventilate the plate cover or shed for 1 hour, and spray clear water on the leaves every morning and evening, spray 50% carbendazim diluted 500 times every 3 days, and remove the shading device and the hole plate cover or shed after 3 days of shading; the cuttings start to take root within a week, A large number of roots after two weeks; as shown in Figure 1-2, Fig. 1 is the root system diagram that grows after two weeks of cuttings of medicinal cannabis branches in embodiment 1, and Fig. 2 is the medicinal cannabis after two weeks of cuttings in embodiment 1 The shape of the branches.

According to the statistics of the test results, the cutting medicinal cannabis branches propagated in this example have a fast rooting speed, and it can be observed that there are roots growing from the lower end of the morphology in about a week, and a large number of roots are observed growing in about two weeks, and the survival rate of cutting seedlings is 100%. . It is proved that this embodiment can effectively carry out the cutting propagation of medicinal cannabis, the rooting speed is fast and the survival rate is high, the survival rate is as high as 100%, and the seedling cultivation period is significantly shortened, and the floor area is small, and the transplanting is convenient, which greatly reduces the time and labor costs.

Claims (10)

1. A cutting method of medicinal cannabis, characterized in that the cutting method is carried out in the following steps: Step 1. Select a medicinal marijuana branch with two stem nodes at the top of a branch that is not fully lignified or semi-lignified, cut the morphological lower end of the branch obliquely, and cut the morphological upper end flat, and the branch retains a stem node, remove The length of the branch after the terminal bud is 5-6cm; the morphological lower end of the pruned branch is placed in the rooting solution diluted 1000 times and soaked for 30-60s to obtain the soaked medicinal cannabis branch; the rooting solution diluted 1000 times The massfraction of naphthaleneacetic acid in the liquid is 2.5%, and the massfraction of indolebutyric acid is 2.5%; Step 2: Add water to the matrix, then sterilize at 121°C for 15-20 minutes, cool the sterilized matrix, put it into a container for compaction, and dig a hole in the center of the matrix; Step 3. Insert the soaked medicinal marijuana branches in step 1 into the hole dug in step 2 and compact it with the substrate, then spray with 50% carbendazim diluted 500 times, and then spray at a temperature of 25-28°C and humidity Place it in shading for 3 days under the condition of 70-85%, and continue to cultivate for 1-2 weeks after the shading ends, and complete the cuttings of medicinal marijuana; during the cultivation period, spray 50% carbendazim diluted 500 times every 3 days. 2. The cutting method of a kind of medicinal marijuana according to claim 1, characterized in that in step 1, the lower end of branch morphology is cut with scissors sterilized with alcohol at 45 ° oblique mouth, and the upper end of branch morphology is cut with flat mouth. 3. The cutting method of a kind of medicinal marijuana according to claim 1, wherein the young leaves of the medicinal marijuana branches are kept when pruning in step 1, and half of the old leaves are cut off. 4. the cutting method of a kind of medicinal cannabis according to claim 1, is characterized in that the matrix in step 2 is made up of carbon soil, vermiculite, perlite and water. 5. A cutting method of medicinal cannabis according to claim 4, characterized in that the mass ratio of charcoal, vermiculite and perlite is 3:1:1. 6. The cutting method of a kind of medicinal marijuana according to claim 1, characterized in that the container adopted in step 2 is a plug. 7. The cutting method of a kind of medicinal cannabis according to claim 1, characterized in that in step 2, water is added to the substrate, then placed in an autoclave, and sterilized at a temperature of 121°C for 15 ~20min. 8. The cutting method of a kind of medicinal cannabis according to claim 1, characterized in that the depth of the hole dug in step 2 is 3-5 cm. 9. The cutting method of a kind of medicinal marijuana according to claim 1, characterized in that in step 3, the depth of inserting the soaked medicinal marijuana branch into the hole is 3-5 cm. 10. The cutting method of medicinal cannabis according to claim 1, characterized in that in step 3, during the cultivation period, ventilation is carried out for 1-2 hours every morning, and clear water is sprayed on the leaves every morning and evening.

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