CN115989756A - Cutting method of medicinal cannabis - Google Patents

Cutting method of medicinal cannabis Download PDF

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Publication number
CN115989756A
CN115989756A CN202211604032.3A CN202211604032A CN115989756A CN 115989756 A CN115989756 A CN 115989756A CN 202211604032 A CN202211604032 A CN 202211604032A CN 115989756 A CN115989756 A CN 115989756A
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China
Prior art keywords
branch
medicinal
cannabis
substrate
cuttage
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CN202211604032.3A
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Chinese (zh)
Inventor
谢姿雁
徐志超
孙伟
陈姗姗
米要磊
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Northeast Forestry University
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Northeast Forestry University
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

Abstract

A cutting method of medicinal cannabis relates to the technical field of plant cultivation. The invention aims to solve the problems of low rooting speed, low survival rate, long breeding time and high labor cost in the conventional breeding of medicinal cannabis sativa. The method comprises the following steps: placing the trimmed morphological lower end of the branch into rooting liquid which is diluted 1000 times and consists of 2.5% of naphthalene acetic acid and 2.5% of indolebutyric acid, and soaking for 30s; cooling the sterilized substrate, filling the substrate into a container, compacting and digging holes; inserting the soaked medicinal cannabis sativa branches into the dug holes, compacting by using a matrix, spraying by using 50% carbendazim diluted 500 times, shading and standing for 3 days at the temperature of 25 ℃ and the humidity of 70%, and continuously culturing for 1-2 weeks after shading, thereby completing cuttage of medicinal cannabis sativa; during the incubation period, 50% carbendazim diluted 500-fold was sprayed every 3 d. The invention can obtain a cuttage method of medicinal cannabis.

Description

Cutting method of medicinal cannabis
Technical Field
The invention relates to the technical field of plant cultivation, in particular to a cutting method of medicinal cannabis.
Background
Industrial cannabis is an annual herb of the cannabis genus of the cannabis family, a hermaphroditic strain, widely distributed worldwide. Industrial hemp is an important commercial crop and is widely used in textile and medical fields. The female flowers and leaves of industrial hemp contain abundant cannabinoids, of which Tetrahydrocannabinol (THC) and Cannabidiol (CBD) are the main two cannabinoids. Tetrahydrocannabinol (THC) has a magic activity, whereas Cannabidiol (CBD) is a non-psychoactive ingredient and has a rich pharmacological activity. The medicinal cannabis refers to a cannabis variety with high Cannabidiol (CBD) content and Tetrahydrocannabinol (THC) content of less than 0.3%, and because the Cannabidiol (CBD) has important medicinal value, the current market demand for the medicinal cannabis has high water-rise, so that the medicinal cannabis is particularly important for efficient breeding. However, the current breeding of the medicinal cannabis has the defects of low rooting speed, low survival rate, long breeding time, high labor cost and the like.
Disclosure of Invention
The invention aims to solve the problems of low rooting speed, low survival rate, long breeding time and high labor cost in the conventional breeding of medicinal cannabis sativa, and provides a cuttage method of medicinal cannabis sativa.
A cutting method of medicinal cannabis sativa comprises the following steps:
firstly, selecting a medicinal hemp branch with two stem knots at the top end of an incompletely lignified or semi-lignified branch, cutting an inclined opening at the morphological lower end of the branch and a flat opening at the morphological upper end of the branch, keeping one stem knot of the branch, and removing the terminal bud, wherein the length of the branch is 5-6 cm; soaking the trimmed morphological lower end of the branch in rooting liquid diluted 1000 times for 30-60 s to obtain soaked medicinal hemp branch; the mass fraction of the naphthylacetic acid in the rooting liquid diluted 1000 times is 2.5%, and the mass fraction of the indolebutyric acid is 2.5%;
adding water into the substrate, sterilizing at 121 ℃ for 15-20 min, cooling the sterilized substrate, filling the cooled substrate into a container, compacting, and digging a hole in the center of the substrate;
inserting the soaked medicinal hemp branches in the first step into the holes dug in the second step, compacting by using a matrix, spraying by using 50% carbendazim diluted 500 times, shading and standing for 3 days at the temperature of 25-28 ℃ and the humidity of 70-85%, and culturing for 1-2 weeks after shading, so as to finish cutting of medicinal hemp; during the incubation period, 50% carbendazim diluted 500-fold was sprayed every 3 d.
The invention has the beneficial effects that:
the invention discloses a cuttage method of medicinal cannabis: the semi-lignified shoots of the medicinal cannabis sativa are used as materials, each cuttage branch is 5-6cm, one stem node is reserved, the tender leaves can be reserved, half of the old leaves are cut off, and terminal buds are removed. The soaking time of the branches in rooting liquid (NAA 2.5% of naphthylacetic acid and IBA2.5% of indolebutyric acid) of 1000 times of liquid is 30-60 s, the NAA can induce the formation of adventitious roots, the IBA can induce the formation of root protoplasm, the rooting liquid is used for promoting the rooting of cuttings, and the germination of the roots can be promoted through the combination of two auxins. The method utilizes the high-pressure sterilization method, has better effect of removing pathogenic bacteria in soil, and avoids the invasion of soil-borne bacteria on cutting branches. In addition, 500 times of liquid 50% carbendazim is used for spraying the cutting seedlings and the environment where the cutting seedlings are positioned, including the inside of the plug cover or the arch shed, and the cutting seedlings and the matrix, so that the contact between branch wounds and environmental germs is avoided, and the survival rate of the cutting branches can be greatly improved. Shading for three days after cutting, and ventilating for 1-2 hours in the morning, so as to keep the activity of the branches. By the method, a high-efficiency medicinal cannabis sativa cutting system is established, the occupied area is small, and the method is suitable for small-amount cutting propagation for scientific research. The test results prove that the rooting speed of the cuttage branches propagated by the method is high, the growth of roots at the morphological lower end can be observed about one week, a large number of roots can be observed about two weeks, the length is about 1cm or more, and the survival rate of cuttage seedlings is 100%. The invention can effectively conduct cuttage propagation of the medicinal cannabis, and has the advantages of high rooting speed, high survival rate and high survival rate up to 100 percent. The seedling raising period is obviously shortened, the occupied area is small, the transplanting is convenient, and the time and labor cost are greatly reduced.
The invention can obtain a cuttage method of medicinal cannabis.
Drawings
FIG. 1 is a graph showing root system of the hemp branch for two weeks after cutting;
FIG. 2 shows the morphology of the medicinal cannabis branch after two weeks of cutting in example 1.
Detailed Description
The first embodiment is as follows: the cuttage method of the medicinal cannabis in the embodiment is carried out according to the following steps:
firstly, selecting a medicinal hemp branch with two stem knots at the top end of an incompletely lignified or semi-lignified branch, cutting an inclined opening at the morphological lower end of the branch and a flat opening at the morphological upper end of the branch, keeping one stem knot of the branch, and removing the terminal bud, wherein the length of the branch is 5-6 cm; soaking the trimmed morphological lower end of the branch in rooting liquid diluted 1000 times for 30-60 s to obtain soaked medicinal hemp branch; the mass fraction of the naphthylacetic acid in the rooting liquid diluted 1000 times is 2.5%, and the mass fraction of the indolebutyric acid is 2.5%;
adding water into the substrate, sterilizing at 121 ℃ for 15-20 min, cooling the sterilized substrate, filling the cooled substrate into a container, compacting, and digging a hole in the center of the substrate;
inserting the soaked medicinal hemp branches in the first step into the holes dug in the second step, compacting by using a matrix, spraying by using 50% carbendazim diluted 500 times, shading and standing for 3 days at the temperature of 25-28 ℃ and the humidity of 70-85%, and culturing for 1-2 weeks after shading, so as to finish cutting of medicinal hemp; during the incubation period, 50% carbendazim diluted 500-fold was sprayed every 3 d.
The second embodiment is as follows: the present embodiment differs from the specific embodiment in that: in the first step, the lower morphological end of the branch is sheared into 45-degree bevel openings by scissors sterilized by alcohol, and the upper morphological end is sheared into flat openings.
The other steps are the same as in the first embodiment.
And a third specific embodiment: the present embodiment differs from the first or second embodiment in that: in the first step, tender leaves of the medicinal hemp branches are reserved during pruning, and half of old leaves are pruned.
Other steps are the same as those of the first or second embodiment.
The specific embodiment IV is as follows: one difference between this embodiment and the first to third embodiments is that: the matrix in the second step consists of charcoal soil, vermiculite, perlite and water.
Other steps are the same as those of the first to third embodiments.
Fifth embodiment: one to four differences between the present embodiment and the specific embodiment are: the mass ratio of the charcoal soil to the vermiculite to the perlite is 3:1:1.
other steps are the same as those of the first to fourth embodiments.
Specific embodiment six: the present embodiment differs from the first to fifth embodiments in that: the container adopted in the second step is a plug.
Other steps are the same as those of the first to fifth embodiments.
Seventh embodiment: one difference between the present embodiment and the first to sixth embodiments is that: and step two, adding water into the matrix, then placing the matrix into an autoclave, and sterilizing the matrix for 15 to 20 minutes at the temperature of 121 ℃.
Other steps are the same as those of embodiments one to six.
Eighth embodiment: one difference between the present embodiment and the first to seventh embodiments is that: and step two, the depth of the dug hole is 3-5 cm.
Other steps are the same as those of embodiments one to seven.
Detailed description nine: one of the differences between this embodiment and the first to eighth embodiments is: and step three, inserting the soaked medicinal hemp branches into the holes to a depth of 3-5 cm.
Other steps are the same as those of embodiments one to eight.
Detailed description ten: the present embodiment differs from the first to ninth embodiments in that: and step three, ventilation is carried out for 1-2 hours in the morning and fresh water is sprayed to the leaf surfaces in the morning and evening every day during the culture period.
Other steps are the same as those of embodiments one to nine.
The following examples are used to verify the benefits of the present invention:
example 1: a cutting method of medicinal cannabis sativa comprises the following steps:
firstly, selecting a medicinal hemp branch with two stem knots at the top end of an incompletely lignified branch or a semi-lignified branch, shearing a 45-degree bevel by scissors with alcohol sterilized at the lower morphological end of the branch, shearing a flat mouth at the upper morphological end of the branch, reserving one stem knot of the branch, reserving tender leaves, shearing half of old leaves, and removing terminal buds, wherein the length of the branch is 5-6 cm; soaking the trimmed morphological lower end of the branch in rooting liquid diluted 1000 times for 30s to obtain soaked medicinal hemp branches;
the mass fraction of the naphthylacetic acid in the rooting liquid diluted 1000 times is 2.5%, and the mass fraction of the indolebutyric acid is 2.5%.
Step two, the substrate is prepared according to peat soil, vermiculite and perlite 3:1:1, adding a proper amount of water, wherein the wettability of the matrix is standardized by water seepage by hand, agglomeration by loose hands and falling to the ground; then placing in an autoclave, sterilizing at 121deg.C for 20min, cooling the sterilized matrix, placing into clean tray, compacting, and punching a hole slightly larger than diameter of medicinal hemp branch at the center of the matrix, wherein the depth of the hole is 3cm.
Vertically inserting the soaked medicinal hemp branches into the holes dug in the second step for 3cm, compacting by using a matrix, covering the hole tray with the hole tray cover or building a small arch shed, uniformly spraying the hole tray cover or the arch shed with 50% carbendazim diluted 500 times, and the medicinal hemp branches and the matrix, and then shading and placing under the conditions of 25 ℃ and 70% humidity after the completion; during the cultivation period, opening the plug cover or the arch shed for ventilation for 1h in the morning and spraying clear water to the leaf surfaces in the morning and evening every day, spraying 50% carbendazim diluted 500 times every 3d, shading and placing for 3 days, and removing the shading device and the plug cover or the arch shed; rooting is started after one week of cuttage, and a large amount of rooting is performed after two weeks; as shown in fig. 1-2, fig. 1 is a root system diagram of the medicinal cannabis sativa branches of example 1 grown after two weeks of cutting, and fig. 2 is a morphology of the medicinal cannabis sativa branches of example 1 after two weeks of cutting.
The test results prove that the rooting speed of the cuttage medicinal cannabis sativa branches propagated by the embodiment is high, the growth of roots at the morphological lower end can be observed about one week, a large number of roots can be observed about two weeks, and the survival rate of cuttage seedlings is 100%. The embodiment proves that the cutting propagation of the medicinal cannabis can be efficiently carried out, the rooting speed is high, the survival rate is high and is up to 100%, the seedling period is obviously shortened, the occupied area is small, the transplanting is convenient, and the time and the labor cost are greatly reduced.

Claims (10)

1. A cutting method of medicinal cannabis is characterized by comprising the following steps:
firstly, selecting a medicinal hemp branch with two stem knots at the top end of an incompletely lignified or semi-lignified branch, cutting an inclined opening at the morphological lower end of the branch and a flat opening at the morphological upper end of the branch, keeping one stem knot of the branch, and removing the terminal bud, wherein the length of the branch is 5-6 cm; soaking the trimmed morphological lower end of the branch in rooting liquid diluted 1000 times for 30-60 s to obtain soaked medicinal hemp branch; the mass fraction of the naphthylacetic acid in the rooting liquid diluted 1000 times is 2.5%, and the mass fraction of the indolebutyric acid is 2.5%;
adding water into the substrate, sterilizing at 121 ℃ for 15-20 min, cooling the sterilized substrate, filling the cooled substrate into a container, compacting, and digging a hole in the center of the substrate;
inserting the soaked medicinal hemp branches in the first step into the holes dug in the second step, compacting by using a matrix, spraying by using 50% carbendazim diluted 500 times, shading and standing for 3 days at the temperature of 25-28 ℃ and the humidity of 70-85%, and culturing for 1-2 weeks after shading, so as to finish cutting of medicinal hemp; during the incubation period, 50% carbendazim diluted 500-fold was sprayed every 3 d.
2. The cuttage method of cannabis sativa as in claim 1, wherein in the first step, the morphological lower end of the branches is cut into 45-degree bevel openings by scissors sterilized by alcohol, and the morphological upper end is cut into flat openings.
3. The cuttage method of cannabis sativa as claimed in claim 1, wherein the tender leaves of the branches of cannabis sativa are remained and half of the old leaves are cut off during the pruning in the first step.
4. The cuttage method of cannabis sativa as claimed in claim 1, wherein the matrix in the second step is composed of charcoal soil, vermiculite, perlite and water.
5. The cuttage method of medicinal cannabis as claimed in claim 4, wherein the mass ratio of the charcoal soil, the vermiculite and the perlite is 3:1:1.
6. the cuttage method of cannabis as claimed in claim 1, wherein the container used in the second step is a tray.
7. The cuttage method of cannabis as claimed in claim 1, wherein in the second step, water is added into the substrate, and then the substrate is placed in an autoclave and sterilized at 121 ℃ for 15-20 min.
8. The cuttage method of cannabis as claimed in claim 1, wherein the depth of the hole dug in the second step is 3-5 cm.
9. The cuttage method of medicinal hemp according to claim 1, wherein the depth of the soaked medicinal hemp branches inserted into the holes in the step three is 3-5 cm.
10. A cutting method of cannabis sativa as claimed in claim 1, wherein in step three, ventilation is performed for 1-2 hours in the morning and fresh water is sprayed to the foliage in the morning and evening every day during the cultivation period.
CN202211604032.3A 2022-12-13 2022-12-13 Cutting method of medicinal cannabis Pending CN115989756A (en)

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Cited By (2)

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CN117016391A (en) * 2023-09-05 2023-11-10 东北林业大学 Method for culturing industrial adventitious roots of cannabis sativa for efficiently producing cannabidiol and polysaccharide
CN117016391B (en) * 2023-09-05 2024-05-03 东北林业大学 Method for culturing industrial adventitious roots of cannabis sativa for producing cannabidiol and polysaccharide

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Publication number Priority date Publication date Assignee Title
CN117016391A (en) * 2023-09-05 2023-11-10 东北林业大学 Method for culturing industrial adventitious roots of cannabis sativa for efficiently producing cannabidiol and polysaccharide
CN117016391B (en) * 2023-09-05 2024-05-03 东北林业大学 Method for culturing industrial adventitious roots of cannabis sativa for producing cannabidiol and polysaccharide

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