CN105230465A - Industrial marijuana soilless rapid propagation method - Google Patents
Industrial marijuana soilless rapid propagation method Download PDFInfo
- Publication number
- CN105230465A CN105230465A CN201510759413.2A CN201510759413A CN105230465A CN 105230465 A CN105230465 A CN 105230465A CN 201510759413 A CN201510759413 A CN 201510759413A CN 105230465 A CN105230465 A CN 105230465A
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- nutrient solution
- cuttings
- liquid
- marijuana
- root
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses an industrial marijuana soilless rapid propagation method and belongs to the technical field of marijuana cultivation and breeding. The method includes the steps that final singling is carried out on plants every 1m<2> or 2m<2> when seedlings are 20 cm high, 2 cm of top shoots are cut when the plants are 1 m high, and 2 cm of top shoots are moved when branches grow to 20 cm high; when the branches grow to 15 cm or 20 cm high, 15 cm or 18 cm of cuttings are cut through a sharp knife, and 2 or 3 leaves which are not completely unfolded on the top are left and soaked in a potassium permanganate solution with the weight ration being 1/5000 for 20 min or 30 min; the portions, 2 cm away from base portions of the cuttings, are wrapped with sponge and planted into cultivation holes of liquid culture tubes; a nutrient solution is prepared through tap water according to requirements, 2-5 L of the nutrient solution is used, the nutrient solution circulates between the liquid culture tubes and a liquid storage barrel through a circulating device, circulation of the nutrient solution is controlled to stop for 20 min every time the nutrient solution circulates for 20 min through a timer, the nutrient solution is replaced once every 10 days, and a ventilating, light-permeable and rainproof environment is required; the cuttings start to root after 10 or 15 days and completely root after 20 or 25 days. The method has the advantages that industrialized seedling production can be achieved, operation is easy, cost is low, stand establishment is quick, marijuana seedlings grow in order, and the method can be widely applied to industrial marijuana cultivation and breeding and relative scientific researches.
Description
Technical field:
The present invention relates to a kind of method numerous soon without soil of industrial hemp, belong to hemp cultivation and breeding technical field.
Background technology:
The application of industrial hemp relates to ecological health food, weaving, biological engineering material, papermaking, military supplies and other light industry field, and recent domestic development rapidly.Industrial hemp is dioecism cross-pollinatd plant, and in kind, interindividual variation is comparatively large, brings adverse effect to culture and utility and scientific experimentation.The seminal propagation fiber crops seedling genotype of industrial hemp is inconsistent, and the cuttings of soil cottage propagation easily go mouldy rotten, rooting rate is on the low side and easily affected by environment and fluctuate.
The method numerous soon without soil of industrial hemp provided by the invention, by literature search, has no the open report identical with the present invention.
Summary of the invention:
The object of the invention is to the deficiency overcoming prior art, provide a kind of result of the test accuracy and scientific industrial hemp of improving without the numerous soon method of soil.
The present invention cultivates the consistent hemp seedling of lots of genes type by the method that Camless valve train is bred, to meet the needs of industrial hemp exact science test to the single test material of heredity.Research finds: industrial hemp cuttings is cultivated in nutrient solution, and at guarantee nutrient solution under appropriate and liquid-circulating condition, start to take root after cuttings 10d or 15d, after 20d or 25d, rooting rate reaches 90% or 100%, a transplantation of seedlings or carry out scientific experimentation under liquid training condition.The identical plant of more than 100 or 150 genotype can be obtained through breeding by a strain industrial hemp.
Industrial hemp of the present invention without native quick-breeding method, its concrete steps are as follows:
1. sow hemp, every 1m during seedling height 20cm when 5cm place, underground soil temperature is stabilized in 8 DEG C
2or 2m
2final singling 1 strain, pinches when plant height 1m, removes top tip 2cm, promotes branch growth, branch growth to removing the branch top tip 2cm during 20cm, point branch needed for acquisition;
2. be used for Camless valve train nursery at branch growth to during 15cm or 20cm, use sharp secateurs or scissor cut cuttings, length 15cm or 18cm, 2, reservation top or 3 not yet full extension blade, remove unnecessary blade and petiole, in the liquor potassic permanganate of weight ratio 1/5000, soak 20min or 30min;
3., apart from cuttings base portion 2cm place sponge wrapping, implant in the culture hole of liquid management, in the nutrient solution that cuttings base portion is stretched in pipe; Prepare nutrient solution with running water, often liter of nutrient solution contains: 303mgKNO
3, 222mgCaCl
2, 246.5mgMgSO
47H
2o, 68mgKH
2pO
4, 27.8mgFeSO
47H
2o, 37.3mgNa
2-EDTA, 9.92mgH
3bO
3, 13.86mgMnCl
24H
2o, 3.16mgZnSO
47H
2o, 0.75mgCuSO
45H
2o, 0.29mgNa
2moO
42H
2o;
4. with commercial EGR, nutrient solution is circulated between liquid management and the liquid storing barrel of black, shading is wrapped up to the culture hole masking foil not implanting cuttings, nutrient solution consumption 25L, Timer Controlling nutrient solution often circulates 20min, static 20min, every 10d changes nutrient solution 1 time, and cuttings liquid is trained the environmental requirement ventilation and penetrating light of taking root and taken shelter from rain;
5. hemp cuttings liquid starts to take root after cultivating 10d or 15d, takes root completely, play transplantation of seedlings or directly under liquid training condition, carry out scientific experimentation after 20d or 25d.
The nutrient solution circulating device used in the present invention is commercial, comprises water pump, flexible pipe, the storage nutrient solution tank of black and timer.Support and the liquid management be placed on support are prepare according to a conventional method with commercial material: liquid management selects the pvc pipe of diameter 10cm or 12cm, body every the culture hole of 8cm or 10cm equidistant perforate footpath 2cm, liquid management length 2m; Supporting structure is conventional structure, and size is optionally determined.
The invention has the advantages that:
1. by obtaining the single seedling of lots of genes fast without native quick-breeding method, overcome the numb seedling genotype of seminal propagation in prior art inconsistent, the cuttings of soil cottage propagation easily go mouldy rotten, rooting rate is on the low side and easily affected by environment and shortcoming that is fluctuation, improves reliability and the repeatability of scientific experimentation.
2. the method embodies advanced Plant breeding techniques; there is the feature of batch production, intensive, scale and precision; simple to operate, cost is low, seedling is fast; the numb seedling growth of breeding is neat, hereditary basis is consistent, can be widely used among industrial hemp cultivation, breeding and relevant scientific research.
Embodiment:
Industrial hemp of the present invention without native quick-breeding method, its concrete steps are as follows:
1. sow hemp, every 1m during seedling height 20cm when 5cm place, underground soil temperature is stabilized in 8 DEG C
2or 2m
2final singling 1 strain, pinches when plant height 1m, removes top tip 2cm, promotes branch growth, branch growth to removing the branch top tip 2cm during 20cm, point branch needed for acquisition;
2. be used for Camless valve train nursery at branch growth to during 15cm or 20cm, use sharp secateurs or scissor cut cuttings, length 15cm or 18cm, 2, reservation top or 3 not yet full extension blade, remove unnecessary blade and petiole, in the liquor potassic permanganate of weight ratio 1/5000, soak 20min or 30min;
3., apart from cuttings base portion 2cm place sponge wrapping, implant in the culture hole of liquid management, in the nutrient solution that cuttings base portion is stretched in pipe; Prepare nutrient solution with running water, often liter of nutrient solution contains: 303mgKNO
3, 222mgCaCl
2, 246.5mgMgSO
47H
2o, 68mgKH
2pO
4, 27.8mgFeSO
47H
2o, 37.3mgNa
2-EDTA, 9.92mgH
3bO
3, 13.86mgMnCl
24H
2o, 3.16mgZnSO
47H
2o, 0.75mgCuSO
45H
2o, 0.29mgNa
2moO
42H
2o;
4. with commercial EGR, nutrient solution is circulated between liquid management and the liquid storing barrel of black, shading is wrapped up to the culture hole masking foil not implanting cuttings, nutrient solution consumption 25L, Timer Controlling nutrient solution often circulates 20min, static 20min, every 10d changes nutrient solution 1 time, and cuttings liquid is trained the environmental requirement ventilation and penetrating light of taking root and taken shelter from rain;
5. hemp cuttings liquid starts to take root after cultivating 10d or 15d, takes root completely, play transplantation of seedlings or directly under liquid training condition, carry out scientific experimentation after 20d or 25d.
The nutrient solution circulating device used in the present invention is commercial, comprises water pump, flexible pipe, the storage nutrient solution tank of black and timer.Support and the liquid management be placed on support are prepare according to a conventional method with commercial material: liquid management selects the pvc pipe of diameter 10cm or 12cm, body every the culture hole of 8cm or 10cm equidistant perforate footpath 2cm, liquid management length 2m; Supporting structure is conventional structure, and size is optionally determined.
The method has been used in industrial hemp breeding by inventor.Facts have proved, the method is simple to operate, cost is low, seedling is fast, the numb seedling growth of breeding is neat, hereditary basis is consistent, inconsistent and the hemp soil cuttage of numb seedling genotype that effectively can overcome cannabis seeds breeding is easily gone mouldy rotten, that rooting rate is unstable shortcoming, be fast, the efficient method for optimizing obtaining the consistent industrial hemp seedling of hereditary basis.
Claims (1)
1. a method numerous soon without soil for industrial hemp, is characterized in that the concrete steps of the method are as follows:
A. hemp is sowed, every 1m during seedling height 20cm when 5cm place, underground soil temperature is stabilized in 8 DEG C
2or 2m
2final singling 1 strain, pinches when plant height 1m, removes top tip 2cm, promotes branch growth, branch growth to removing the branch top tip 2cm during 20cm, point branch needed for acquisition;
B. Camless valve train nursery is used at branch growth to during 15cm or 20cm, use sharp secateurs or scissor cut cuttings, length 15cm or 18cm, 2, reservation top or 3 not yet full extension blade, remove unnecessary blade and petiole, in the liquor potassic permanganate of weight ratio 1/5000, soak 20min or 30min;
C. apart from cuttings base portion 2cm place sponge wrapping, implant in the culture hole of liquid management, in the nutrient solution that cuttings base portion is stretched in pipe; Prepare nutrient solution with running water, often liter of nutrient solution contains: 303mgKNO
3, 222mgCaCl
2, 246.5mgMgSO
47H
2o, 68mgKH
2pO
4, 27.8mgFeSO
47H
2o, 37.3mgNa
2-EDTA, 9.92mgH
3bO
3, 13.86mgMnCl
24H
2o, 3.16mgZnSO
47H
2o, 0.75mgCuSO
45H
2o, 0.29mgNa
2moO
42H
2o;
D. with commercial EGR, nutrient solution is circulated between liquid management and the liquid storing barrel of black, shading is wrapped up to the culture hole masking foil not implanting cuttings, nutrient solution consumption 25L, Timer Controlling nutrient solution often circulates 20min, static 20min, every 10d changes nutrient solution 1 time, and cuttings liquid is trained the environmental requirement ventilation and penetrating light of taking root and taken shelter from rain;
E. hemp cuttings liquid starts to take root after cultivating 10d or 15d, takes root completely, play transplantation of seedlings or directly under liquid training condition, carry out scientific experimentation after 20d or 25d.
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| CN201510759413.2A CN105230465A (en) | 2015-11-10 | 2015-11-10 | Industrial marijuana soilless rapid propagation method |
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|---|---|---|---|
| CN201510759413.2A CN105230465A (en) | 2015-11-10 | 2015-11-10 | Industrial marijuana soilless rapid propagation method |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106900505A (en) * | 2017-03-03 | 2017-06-30 | 福建省中科生物股份有限公司 | A kind of artificial light environment cultural method of hemp |
| CN108934803A (en) * | 2018-04-12 | 2018-12-07 | 中国农业科学院麻类研究所 | A method of cultivating hemp seedling |
| CN112514783A (en) * | 2019-09-19 | 2021-03-19 | 黑龙江汉美生工业大麻科技有限公司 | Method for planting organic industrial hemp in non-woven fabric beauty planting bag |
| WO2022104862A1 (en) * | 2020-11-18 | 2022-05-27 | 福建省中科生物股份有限公司 | Method for promoting rooting of industrial hemp |
| GR1010407B (en) * | 2022-03-28 | 2023-02-16 | Ekati Alchemy Lab Sl, | Novel in vitro propagation method for the preservation and conservation of cannabis species plants |
| CN115989756A (en) * | 2022-12-13 | 2023-04-21 | 东北林业大学 | Cutting method of medicinal cannabis |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102919044A (en) * | 2012-11-22 | 2013-02-13 | 云南大学 | Cutting propagation method of industrial cannabis sativa |
| CN103069949A (en) * | 2013-02-01 | 2013-05-01 | 云南省农业科学院经济作物研究所 | Method for improving germination rate of wild hemp seeds |
| CN103416206A (en) * | 2013-08-20 | 2013-12-04 | 山西省农业科学院经济作物研究所 | Improved variety breeding technology for industrial cannabis sativa L. |
| CN103621392A (en) * | 2013-12-23 | 2014-03-12 | 云南大学 | Method for cultivating industrial hemp liquid |
-
2015
- 2015-11-10 CN CN201510759413.2A patent/CN105230465A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102919044A (en) * | 2012-11-22 | 2013-02-13 | 云南大学 | Cutting propagation method of industrial cannabis sativa |
| CN103069949A (en) * | 2013-02-01 | 2013-05-01 | 云南省农业科学院经济作物研究所 | Method for improving germination rate of wild hemp seeds |
| CN103416206A (en) * | 2013-08-20 | 2013-12-04 | 山西省农业科学院经济作物研究所 | Improved variety breeding technology for industrial cannabis sativa L. |
| CN103621392A (en) * | 2013-12-23 | 2014-03-12 | 云南大学 | Method for cultivating industrial hemp liquid |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106900505A (en) * | 2017-03-03 | 2017-06-30 | 福建省中科生物股份有限公司 | A kind of artificial light environment cultural method of hemp |
| CN108934803A (en) * | 2018-04-12 | 2018-12-07 | 中国农业科学院麻类研究所 | A method of cultivating hemp seedling |
| CN112514783A (en) * | 2019-09-19 | 2021-03-19 | 黑龙江汉美生工业大麻科技有限公司 | Method for planting organic industrial hemp in non-woven fabric beauty planting bag |
| WO2022104862A1 (en) * | 2020-11-18 | 2022-05-27 | 福建省中科生物股份有限公司 | Method for promoting rooting of industrial hemp |
| GR1010407B (en) * | 2022-03-28 | 2023-02-16 | Ekati Alchemy Lab Sl, | Novel in vitro propagation method for the preservation and conservation of cannabis species plants |
| CN115989756A (en) * | 2022-12-13 | 2023-04-21 | 东北林业大学 | Cutting method of medicinal cannabis |
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Application publication date: 20160113 |