CN109496824A - Collect the hydroponic device and ciltivating process of cultivated plant root rhizosphere secreta - Google Patents
Collect the hydroponic device and ciltivating process of cultivated plant root rhizosphere secreta Download PDFInfo
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- 230000008569 process Effects 0.000 title claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 102
- 235000015097 nutrients Nutrition 0.000 claims abstract description 13
- 230000028327 secretion Effects 0.000 claims description 15
- 238000011010 flushing procedure Methods 0.000 claims description 3
- 210000003296 saliva Anatomy 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 9
- 229910052760 oxygen Inorganic materials 0.000 abstract description 9
- 239000001301 oxygen Substances 0.000 abstract description 9
- 230000008635 plant growth Effects 0.000 abstract description 6
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- 235000007201 Saccharum officinarum Nutrition 0.000 description 15
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- 101100298225 Caenorhabditis elegans pot-2 gene Proteins 0.000 description 11
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- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
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- 239000007788 liquid Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
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- 238000012360 testing method Methods 0.000 description 2
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- 229930003944 flavone Natural products 0.000 description 1
- 150000002213 flavones Chemical class 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
- A01G31/06—Hydroponic culture on racks or in stacked containers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Hydroponics (AREA)
Abstract
The invention discloses a kind of hydroponic devices for collecting cultivated plant root rhizosphere secreta, comprising: laying rack is stair-stepping multilayer layer frame structure；Planting pot is used to hold mill water culture nutrient solution, is placed in the layer frame of the laying rack, and the tub wall of the planting pot is equipped with apopore, and the apopore is connect with outlet pipe；The water outlet of the outlet pipe of the planting pot of a upper layer frame is correspondingly arranged at the basin mouth of the planting pot of next layer frame, set on the top of planting pot basin mouth described in next layer frame；And reservoir, it includes inlet and outlet, the water inlet is connect with the planting pot outlet pipe being set in lowest level layer frame, the water outlet is connect by water pump with water pipe, the water outlet of the water pipe is correspondingly arranged at the basin mouth of the planting pot of top layer's layer frame, positioned at the top of basin mouth.Hydroponic device of the present invention, by being circulated to nutrient solution between planting pot and reservoir, the automatic water-replacing of water planting plantation.Sufficient oxygen is provided for the growth of plant.
Description
Technical field
The present invention relates to field of plant cultivation, in particular to a kind of hydroponic device for collecting cultivated plant root rhizosphere secreta and
Ciltivating process, the hydroponic device are mainly used for collecting the root rhizosphere secreta of macrophyte, such as sugarcane etc..
Background technique
With development in science and technology and social progress, novel agriculture cultivation model is gradually applied and promotes.Water planting conduct
One kind of novel agricultural training method saves fertilizer with it, advantage easy to clean gradually captures market.Meanwhile water culture technique
Application, for people further investigate the micro- domain ecology of plant rhizosphere variation and analysis plant growth main affecting factors provide
Great convenience.
Root exudates refers to the organic matter that plant is discharged by root into growth substrate in growth and development process
General name, including exudate, secretion, cast etc..Plant is with root secretion during the growth process there are about 20% synthetic
Form discharged into growth substrate by root system, the formation of root exudates is life important in plant metabolic process
Phenomenon is managed, is the key factor for adjusting rhizosphere microecological system vigor and function.Organic compound one contained by root exudates
As at 200 kinds or more, be divided into 3 classes, one kind is macromolecular compound, mainly includes sugar, protein, enzyme and gel etc.；One kind is
Small molecule compound mainly includes acid, phenol, ketone etc.；One kind is growth hormone, flavones, steroid etc..Root secretion composition and content
Variation be plant responding environment-stress most directly, most apparent reaction, be that different ecological type plant is long-term to its living environment
The result of adaptation.Plant rhizosphere secretion especially plant root exudation proteomics research is widely closed in recent years
Note.Secretory protein participates in a variety of lifes such as the relevant root system extracellular signal approach of plant, form generation, Apoptosis, cell differentiation
Object process, and root system secretory protein also has chemical activation effect, modification soil and the degeneration-resistant energy for improving plant to soil nutrient
Power.The specific root secretion inhereditary feature important as one, plant nutrient heredity improvement in by people increasingly
Pay attention to, the research of especially secretory protein group has become the key areas of domestic and foreign scholars' Recent study exploration.
The processing that completely collects and analyzes of plant rhizosphere secretion is the premise for obtaining true and reliable result of study.Due to soil
The reasons such as the absorption of earth and soil contained substance are uncontrollable, so being collected by soil potting to secretions from plant roots
In the presence of centainly more difficult.The collection method of plant rhizosphere secretion mainly has direct water planting collecting method at present, and water culture is in root system
Secretion is widely used on collecting, and easy to operate, water planting liquid Composition Control is accurate, reflects certain specific root systems to a certain extent
The variation of secretion, and the interference for excluding edaphon etc..However, due in mill water culture nutrient solution dissolved oxygen content it is low, directly
Water planting collecting method is generally only applicable to that growth increment is smaller, itself oxygen demand also little crop, big for oxygen demand
Plant or the big growth phase root of nutritional requirement are easy because supporting deficiency to cause plant strain growth bad or bad root phenomenon occur.Cause
This, in practical application, root rhizosphere secreta collection method more commonly used at present is to grow into crop in soil matrix centainly
Stage is transplanted in water planting is cultivated in short term again.In this way, which the root rhizosphere secreta being collected into actually can not be sufficiently anti-
Reflect or represent the truth in the micro- domain of plant rhizosphere.Although can certain journey after passing through improvement there are many hydroponic device at present
Aerobic when solving the problems, such as water culture on degree, still, the demand of crop water planting condition biggish for some increments is still
It cannot fully meet.
Sugarcane is annual gramineae crop, has the characteristics that increment is big, suction fertilizer is more, root system is huge, growth cycle is long,
Its growth period is generally up to 12-18 months or even longer.The growth cycle of sugarcane has a stage: budding period, Seedling Stage, tiller
Phase extends phase and maturity period, and sugarcane is since tillering stage, especially in the elongation phase, all to nutritional requirements such as moisture and nutrients
It is very big, the needs of general hydroponic device is difficult to meet sugarcane production after tillering stage.However, it will be appreciated that the rhizosphere of sugarcane is micro-
Domain ecology, the root rhizosphere secreta that at least collect its grand period of growth, which carries out analysis, could obtain with certain representative research
As a result.
The information disclosed in the background technology section is intended only to increase the understanding to general background of the invention, without answering
When being considered as recognizing or imply that the information constitutes the prior art already known to those of ordinary skill in the art in any form.
Summary of the invention
The purpose of the present invention is to provide a kind of hydroponic devices for collecting cultivated plant root rhizosphere secreta, to overcome large size
Secretions from plant roots collection be not easy, the disadvantage of oxygen supply equipment complexity.
Another object of the present invention is to provide a kind of ciltivating process.
To achieve the above object, the present invention provides a kind of hydroponic devices for collecting cultivated plant root rhizosphere secreta, comprising:
Laying rack is stair-stepping multilayer layer frame structure；Planting pot is used to hold mill water culture nutrient solution, is placed in the laying rack
In layer frame, the tub wall of the planting pot is equipped with apopore, and the apopore is connect with outlet pipe；The plantation of a upper layer frame
The water outlet of the outlet pipe of basin is correspondingly arranged at the basin mouth of the planting pot of next layer frame, is set to planting pot described in next layer frame
The top of basin mouth；And reservoir comprising inlet and outlet, the water inlet be set to lowest level layer frame on it is described
The connection of planting pot outlet pipe, the water outlet are connect by water pump with water pipe, and the water outlet of the water pipe is correspondingly arranged at top layer
At the basin mouth of the planting pot of layer frame, positioned at the top of basin mouth.
Preferably, in above-mentioned technical proposal, the laying rack includes: objective table, is used to place the planting pot；And
Telescopic rod, the telescopic rod are connect with the lower surface of the objective table, are used to support the objective table, the telescopic rod is at least
One flexible point.
Preferably, in above-mentioned technical proposal, the telescopic rod includes: support rod, is in hollow structure, sets on the support rod
There is perforation；Motion bar, in mobilizable cavity for being sheathed on the support rod, the motion bar is equipped with multiple fixation holes,
In a state of use, the fixation hole of at least one motion bar and the perforation of the support rod are correspondingly arranged；And fixing piece,
Its perforation for being arranged in the support rod and the fixation hole of the motion bar, for fixing the motion bar and the support rod.
Preferably, in above-mentioned technical proposal, field planting basket is arranged in the planting pot.
Preferably, in above-mentioned technical proposal, the fixed planting pot is made of light-proof material.
Preferably, in above-mentioned technical proposal, the middle and lower part tub wall of the planting pot is equipped with apopore.
A kind of ciltivating process using above-mentioned hydroponic device cultivated plant, comprising the following steps:
(1) plantling of cultivation is extracted, is rinsed with water plant root；
(2) a period of time in water is impregnated into the root of the plantling after flushing；
(3) plantling is transplanted in the field planting basket of planting pot of water planting, the root of plant completely in the bottom of field planting basket,
And be free in culture solution, stem is fixed in field planting basket with fixing piece；
(4) planting pot is placed in the layer frame of laying rack, shelf upper layer planting pot apopore is connected to outlet pipe
On next layer of planting pot, the water outlet of outlet pipe is placed in above next layer of planting pot basin saliva face, and water flow is from upper layer planting pot
Bring air when flowing into lower layer's planting pot into, the water of bottom planting pot flows into reservoir, and the water of reservoir passes through water pump and water pipe
It is introduced into top planting pot, forms water circulation；
(5) culture solution that timed collection contains the culture solution of cultivated plant root rhizosphere secreta, and more renews continues to collect and plant
Object root rhizosphere secreta.
Preferably, in above-mentioned technical proposal, plantling root is impregnated 1-2 days with flowing water in step (2).
Compared with prior art, the invention has the following beneficial effects:
(1) hydroponic device of collection cultivated plant root rhizosphere secreta of the invention, has structurally reasonable simple, water planting quality
The characteristics of height, energy conservation and environmental protection, realizes the automatic of water planting plantation by circulating to nutrient solution between planting pot and reservoir
Water is changed, the efficiency of water planting plantation is effectively improved.Planting pot can be replaced and be adjusted according to the size of water planting crops plant, full
The needs of sufficient different growing stages sugarcane water planting plantation.Meanwhile the height-adjustable water planting by adjusting field planting basket in planting pot is planted
Depth of the object root in nutrient solution guarantees the speed of growth and growth quality of hydroponic plant as needed.Operation of the present invention side
Just, different culture solutions can be added as needed while carrying out multiple and different test process, work efficiency is high, and research is planted
The research that object Rhizosphere environment and analysis main environment factor pair plant growth influence has important practical advice effect.
(2) when the water of hydroponic device of the invention, laying rack upper layer planting pot flows into next layer of planting pot from apopore
Bring air into, the water of bottom planting pot flows into reservoir, and the water of reservoir introduces top plantation by water pump and water pipe
Basin forms water circulation.Sufficient oxygen is provided for the growth of plant, no longer needs to setting oxygen supply equipment.
(3) ciltivating process of the invention, cultural method is simple, convenient to carry out.It can be needed to be precisely controlled training according to test
Nutrient solution, can be with the ecological environment of soil of simulation of plant growth phase, while being suitble to the ciltivating process of the collection of secretions from plant roots.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the hydroponic device according to the present invention for collecting cultivated plant root rhizosphere secreta.
Fig. 2 is the structural representation of telescopic rod in the hydroponic device according to the present invention for collecting cultivated plant root rhizosphere secreta
Figure.
Specific embodiment
With reference to the accompanying drawing, specific embodiments of the present invention will be described in detail, it is to be understood that guarantor of the invention
Shield range is not limited by the specific implementation.
Unless otherwise explicitly stated, otherwise in entire disclosure and claims, term " includes " or its change
Changing such as "comprising" or " including " etc. will be understood to comprise stated element or component, and not exclude other members
Part or other component parts.
As shown in Figure 1, a kind of water planting of collection cultivated plant root rhizosphere secreta of specific embodiment fills according to the present invention
It sets, comprising: laying rack 1, planting pot 2 and reservoir 3.Laying rack 1 is stair-stepping multilayer layer frame structure, the pendulum in the present embodiment
Putting frame is 5 layers of structure, per the high 20-30cm of frame layer by layer.Planting pot 2 is placed in the layer frame of laying rack 1, for holding water planting nutrition
Liquid.Planting pot 2 is made of black non transparent material, round, rectangular or polygon of the shape of planting pot 2 etc., high-order 20-30cm.
Field planting basket 21 is arranged in planting pot 2.The middle and lower part tub wall of planting pot 2 be equipped with apopore 22, water outlet bore dia 2-3mm it
Between, apopore 22 is connect with outlet pipe 23.The water outlet of the outlet pipe of the planting pot of a upper layer frame is correspondingly arranged at next layer frame
At the basin mouth of planting pot, set on the top of next layer frame planting pot basin mouth.Reservoir 3 includes inlet and outlet, reservoir
Water inlet connect with the outlet pipe of the planting pot 2 in lowest level layer frame, 3 water outlet of reservoir is connected by water pump 4 and water pipe 5
It connects, water pump 4 is connect with power supply 6, and power supply 6 is water-proofing power socket.The water outlet of water pipe 5 is correspondingly arranged at the plantation of top layer's layer frame
At the basin mouth of basin, positioned at the top of basin mouth.Wherein, water pipe 5 is hose.
It drives water pump 5 to work by power supply 6, nutrient solution between planting pot 2 and reservoir 3 is circulated, realize water
The automatic water-replacing for cultivating plant effectively improves the efficiency of water planting plantation.Planting pot 2 can be carried out according to the size of water planting crops plant
Replacement and adjustment, meet the needs of different growing stages sugarcane water planting plantation.Meanwhile the height by adjusting field planting basket in planting pot
Adjustable depth of the hydroponic plant root in nutrient solution, guarantees the speed of growth and growth quality of hydroponic plant as needed.
The water of 1 upper layer planting pot of laying rack brings air, the water flow of bottom planting pot into when flowing into next layer of planting pot from apopore
Enter reservoir, the water of reservoir introduces top planting pot by water pump and water pipe, forms water circulation.It is mentioned for the growth of plant
Sufficient oxygen has been supplied, setting oxygen supply equipment is no longer needed to.
A relatively good embodiment about laying rack is that laying rack 1 includes telescopic rod 11 and objective table 12, telescopic rod
11 are set to the lower surface of objective table 12, are used to support objective table 12.There is at least one movable telescopic point 13 on telescopic rod 11.Pass through
The whole height of the flexible adjustable laying rack 1 of telescopic rod 11.It is adjustable and retractable bar without using laying rack 1, the laying rack made
Objective table 12 in a plane, multiple laying racks are stackable to be put, convenient for storage, save space.It is also convenient for tearing open simultaneously
Dress.
Specific embodiment about telescopic rod 11 is, telescopic rod 11 includes hollow support rod 11 and telescopic is sheathed on
Motion bar 112 in support rod.Support rod 11 is equipped with perforation, and motion bar 112 is equipped with multiple fixation holes, is adjusted to needs
After height, the fixation hole in perforation and motion bar 112 on support rod is passed through with fixing piece 113 (fixing piece is fixed pin),
Motion bar 112 is fixed on to certain height.
A kind of ciltivating process using above-mentioned hydroponic device cultivated plant, the present embodiment are specifically included for cultivating sugarcane
Following steps:
(1) the sugarcane seedling of sand culture is pulled up, rinses plant root with circulating water, to obtain than more complete root system；
(2) root of the sugarcane after flushing is immersed in 1-2d in the clear water of flowing, so that its root system can be adapted to preferably
Hydroponic growth conditions；
(3) by the field planting basket of sugarcane transplantation of seedlings to planting pot, the root of sugarcane submerges completely in the bottom of field planting basket
In culture solution, stem is buried with handstone in field planting basket and is fixed；
(4) planting pot is placed in the layer frame of laying rack 1,1 planting pot 2 of every layer of shelf placement, 5 layers of column composition 5
The processing that basin is 1 group repeats, and shelf upper layer planting pot apopore is connected on next layer of planting pot with outlet pipe, outlet pipe
Water outlet is placed in above next layer of planting pot basin saliva face, and water flow brings air into when flowing into lower layer's planting pot from upper layer planting pot,
The water of bottom water planting planting pot 2 flows into reservoir 3, and the water of reservoir is introduced into top plantation by water pump and water pipe
Basin forms water circulation；One shelf can place multiple row, can design multiple processing and repeat；
(5) culture solution that timed collection contains the culture solution of Among the Sugarcane root secretion, and more renews continues to collect sugarcane root
Border secretion.
The aforementioned description to specific exemplary embodiment of the invention is in order to illustrate and illustration purpose.These descriptions
It is not wishing to limit the invention to disclosed precise forms, and it will be apparent that according to the above instruction, can much be changed
And variation.The purpose of selecting and describing the exemplary embodiment is that explaining specific principle of the invention and its actually answering
With so that those skilled in the art can be realized and utilize a variety of different exemplary implementation schemes of the invention and
Various chooses and changes.The scope of the present invention is intended to be limited by claims and its equivalents.
Claims (8)
1. a kind of hydroponic device for collecting cultivated plant root rhizosphere secreta characterized by comprising
Laying rack is stair-stepping multilayer layer frame structure；
Planting pot is used to hold mill water culture nutrient solution, is placed in the layer frame of the laying rack, and the tub wall of the planting pot is equipped with
Apopore, the apopore are connect with outlet pipe；The water outlet of the outlet pipe of the planting pot of a upper layer frame is correspondingly arranged at down
At the basin mouth of the planting pot of one layer frame, set on the top of planting pot basin mouth described in next layer frame；And
Reservoir comprising inlet and outlet, the water inlet are discharged with the planting pot being set in lowest level layer frame
Pipe connection, the water outlet are connect by water pump with water pipe, and the water outlet of the water pipe is correspondingly arranged at the described of top layer's layer frame
At the basin mouth of planting pot, positioned at the top of basin mouth.
2. the hydroponic device according to claim 1 for collecting cultivated plant root rhizosphere secreta, which is characterized in that described to put
Frame includes:
Objective table is used to place the planting pot；And
Telescopic rod, the telescopic rod are connect with the lower surface of the objective table, are used to support the objective table, the telescopic rod is extremely
A rare flexible point.
3. the hydroponic device according to claim 1 for collecting cultivated plant root rhizosphere secreta, which is characterized in that described flexible
Bar includes:
Support rod, is in hollow structure, and the support rod is equipped with perforation；
Motion bar, in mobilizable cavity for being sheathed on the support rod, the motion bar is equipped with multiple fixation holes, makes
With under state, the fixation hole of at least one motion bar and the perforation of the support rod are correspondingly arranged；And
Fixing piece, be arranged in the support rod perforation and the motion bar fixation hole, for fix the motion bar and
The support rod.
4. the hydroponic device according to claim 1 for collecting cultivated plant root rhizosphere secreta, which is characterized in that the plantation
Field planting basket is arranged in basin.
5. the hydroponic device according to claim 1 for collecting cultivated plant root rhizosphere secreta, which is characterized in that the field planting
Basin is made of light-proof material.
6. the hydroponic device according to claim 1 for collecting cultivated plant root rhizosphere secreta, which is characterized in that the plantation
The middle and lower part tub wall of basin is equipped with apopore.
7. a kind of ciltivating process using hydroponic device cultivated plant as described in claim 1, which is characterized in that including following step
It is rapid:
(1) plantling of cultivation is extracted, is rinsed with water plant root；
(2) a period of time in water is impregnated into the root of the plantling after flushing；
(3) plantling is transplanted in the field planting basket of planting pot of water planting, the root of plant is swum completely in the bottom of field planting basket
From in culture solution, stem is fixed in field planting basket with fixing piece；
(4) planting pot is placed in the layer frame of laying rack, shelf upper layer planting pot apopore is connected to outlet pipe next
On the planting pot of layer, the water outlet of outlet pipe is placed in above next layer of planting pot basin saliva face, and the water of bottom planting pot flows into
The water of reservoir, reservoir introduces top planting pot by water pump and water pipe, forms water circulation；
(5) culture solution that timed collection contains the culture solution of cultivated plant root rhizosphere secreta, and more renews continues to collect plant roots
Border secretion.
8. ciltivating process according to claim 7, which is characterized in that impregnate plantling root with flowing water in step (2)
Portion 1-2 days.
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| CN201811644766.8A CN109496824A (en) | 2018-12-29 | 2018-12-29 | Collect the hydroponic device and ciltivating process of cultivated plant root rhizosphere secreta |
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| CN201811644766.8A CN109496824A (en) | 2018-12-29 | 2018-12-29 | Collect the hydroponic device and ciltivating process of cultivated plant root rhizosphere secreta |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110741921A (en) * | 2019-11-30 | 2020-02-04 | 宁波大龙农业科技有限公司 | Three-dimensional water culture device |
| CN111056643A (en) * | 2020-01-13 | 2020-04-24 | 上海世渊环保科技有限公司 | River channel ecological treatment system and treatment method |
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| CN103039339A (en) * | 2012-12-24 | 2013-04-17 | 何寒 | Plant water planting production device |
| CN103759978A (en) * | 2014-02-10 | 2014-04-30 | 金陵科技学院 | Multi-combined device for sterile collection of plant root exudates and application thereof |
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| CN110741921B (en) * | 2019-11-30 | 2021-04-27 | 宁波大龙农业科技有限公司 | Three-dimensional water culture device |
| CN111056643A (en) * | 2020-01-13 | 2020-04-24 | 上海世渊环保科技有限公司 | River channel ecological treatment system and treatment method |
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