CN111418380B

CN111418380B - Illumination culture method for promoting green stalk vegetable and Chinese cabbage heart to increase green

Info

Publication number: CN111418380B
Application number: CN202010295530.9A
Authority: CN
Inventors: 黄烯; 钱崇祯; 毛伟伟
Original assignee: Xiamen University
Current assignee: Xiamen University
Priority date: 2020-04-15
Filing date: 2020-04-15
Publication date: 2022-07-19
Anticipated expiration: 2040-04-15
Also published as: CN111418380A

Abstract

A Chinese medicinal composition for promoting growth of caulis et folium Brassicae Capitatae and caulis et folium Brassicae CapitataeA light culture method for increasing green color relates to the planting culture of green stem vegetable and four-nine heart vegetable. During the growth period of the green stalk vegetable and the four-nine cabbage heart seedlings, the green stalk vegetable and the four-nine cabbage heart seedlings are irradiated by an artificial light source, so that the growth of leaves of the green stalk vegetable and the four-nine cabbage heart seedlings is facilitated, the leaves become green, and chlorophyll is accumulated. The light supply mode of the artificial light source is a light supply mode of conventional white light and low-dose UV-B illumination. The wavelength of low-dose UV-B light is 280-320 nm, and the light quantum flux density is 3 mu mol.m^‑2·s^‑1. Is suitable for the growth of seedlings of full-artificial caulis et folium Brassicae Capitatae and caulis et folium Brassicae Junceae. The illumination method is optimized, the growth of seedlings of the green stem vegetables and the four-nine heart vegetables is promoted, the production period is shortened, and the energy consumption is reduced. The method is simple, is convenient to operate, and has wide application space and market prospect in agricultural modern production.

Description

Illumination culture method for promoting green stalk vegetable and four-nine heart vegetable to increase green

Technical Field

The invention relates to planting and culturing of green stem vegetables and four-nine heart vegetables, in particular to an illumination culturing method for promoting the green stem vegetables and the four-nine heart vegetables to increase green.

Background

Light is one of the most important environmental factors affecting life activities of animals and plants. It is not only an energy source for plant photosynthesis, but also acts as a signaling molecule to regulate multiple processes of plant growth and development. UV-B light is an inherent component of sunlight, high doses of UV-B light cause plant damage, and low doses of UV-B light can serve as a signaling factor to promote the photomorphogenetic response of plants.

An artificial light type plant factory is a facility form which provides a growing light source for crops by means of an artificial light source and has an accurate and adjustable environment. The leaf vegetables have the characteristics of short growth cycle, short plant type, high planting density, convenience for unified management and the like, so the leaf vegetables are ideal vegetable types for artificial light type plant factories from the economic viewpoint. The production regulation and control means which is commonly used in plant factories at present is to improve the yield and the quality of leaf vegetables through reasonable light environment regulation and control. The specific light quality conditions have influence on the vegetable morphology, the chlorophyll content of leaves, the photosynthesis characteristics and the product quality, and the light quality favorable for the growth of vegetable seedlings can be selected according to the specific light quality conditions. The seedlings are irradiated by light with specific wavelength, so that the seedlings can grow according to the preset required indexes after coming out of the soil, and the growth speed is high, and the plant diseases and insect pests are reduced.

Green stalk vegetable and four nine heart vegetables. The green stem vegetable has high vitamin content and rich nutrition, and is one of the favorite vegetables. The sijiu flowering cabbage is tender in texture, fragrant in taste, capable of promoting digestion and popular with the masses. The green stem vegetable and the four-nine heart vegetable can be planted all the year round, but the continuous planting can cause the yield to be reduced and the diseases and insect pests are easy to generate. Therefore, the yield reduction caused by plant diseases and insect pests can be avoided by planting the green-stalk vegetables and the four-nine heart vegetables in a plant factory, for example, patent CN110036896A, "a method for continuous LED light soilless culture of green-stalk vegetables in a climatic chamber", discloses a method for planting green-stalk vegetables. However, the light source in the current artificial light type plant factory is mainly white light, and no UV-B light is involved. Moreover, it is not known how UV-B light affects the growth of green stem vegetables and heart of the four and nine vegetables. There are studies reporting that low doses of UV-B light can promote photomorphogenesis and stress resistance in plants (Jenkins G I. Signal transduction in stresses to UV-B irradiation [ J ]. Annu Rev Plant Biol,2009,60: 407-31; Rizzini L, Favory J J, Cloix C, et al. Perception of UV-B by the Arabidopsis UVR8 protein [ J ]. Science,2011,332(6025): 103-6.). Therefore, when planting green stem vegetables and four-nine heart vegetables in a plant factory, increasing UV-B light on the basis of conventional white light may be used as a scheme for improving yield.

Disclosure of Invention

The invention aims to provide an illumination culture method for promoting green stalk vegetable and four-nine heart cabbage to increase green aiming at the defects in the prior art for planting green stalk vegetable and four-nine heart cabbage.

The method comprises the following specific steps:

during the growth period of the green stem vegetable seedlings and the four-nine cabbage heart seedlings, the green stem vegetable seedlings and the four-nine cabbage heart seedlings are irradiated by an artificial light source, and the light supply mode of the artificial light source is a conventional white light and low-dose UV-B illumination light supply mode.

The light supply mode of the conventional white light and the low-dose UV-B light comprises that in a 24h day-night period, the irradiation time of the conventional white light is 14h, and the low-dose UV-B light is irradiated for 3h at the same time of the conventional white light irradiation.

The irradiation time of the conventional white light can be selected from 08: 00-22: 00; the time of UV-B light irradiation can be selected from 11: 00-14: 00.

The conventional white light has a photon flux density (PPFD) of 80. mu. mol. m^-2·s^-1(ii) a The light quantum flux density of low-dose UV-B illumination is 3 mu mol.m^-2·s^-1。

The wavelength of the low-dose UV-B light is 280-320 nm.

The low-dose UV-B light irradiation time and the light quantum flux density are UV-B light irradiation time and light quantum flux density when sunlight is the strongest in a simulated natural day.

The invention relates to a method for adding low-dose UV-B light on the basis of a conventional white light source in the cultivation process of green stem vegetables and four-nine flowering cabbage in plant factories. A large number of experiments show that during the growth period of green stem vegetables and seedling of the four-nine-heart cabbage, 3h of low-dose UV-B illumination is added every day on the basis of conventional white light, and medium-wave ultraviolet light (UV-B, 280-320 nm) is used as a necessary component of sunlight which can reach the earth surface, so that the low-dose UV-B illumination is beneficial to plant development in the aspects of photomorphogenesis, secondary metabolism, disease and insect resistance and the like. The technical scheme of the invention is beneficial to the growth of the leaves of the seedlings of the green stem vegetables and the four-nine heart vegetables, the green leaves and the accumulation of chlorophyll. The invention is suitable for the growth of seedlings of full-artificial pedunculate cabbage and four-nine cabbage heart. The invention utilizes an optimized illumination method to promote the growth of seedlings of green stem vegetables and four-nine heart vegetables, shorten the production period and reduce the energy consumption. The method is simple, is convenient to operate, and has wide application space and market prospect in agricultural modern production.

Drawings

FIG. 1 is a graph of a white light boost low dose UV-B light exposure test setup in accordance with the present invention.

FIG. 2 is a graph of the growth of seedlings of green stem vegetable and Chinese cabbage heart promoted by white light added with low dose UV-B light irradiation in the present invention.

FIG. 3 is a graph showing the growth of green stem vegetables and four-nine cabbage heart seedlings promoted by white light addition and low dose UV-B light irradiation in the present invention.

FIG. 4 is a graph of the increase of chlorophyll accumulation in seedlings of green stem vegetables and four-nine-cabbage heart plants by white light and low-dose UV-B light irradiation in the present invention.

Detailed Description

The following examples will further illustrate the present invention with reference to the accompanying drawings.

Example 1

The green stem vegetable or the four-nine heart vegetable seedlings with the same specification are separately planted into a culture device and divided into 2 groups, wherein the 1 st group is only irradiated by conventional white light, and the 2 nd group is irradiated by conventional white light and low-dose UV-B light (shown in figure 1). The set light environment parameters are as follows: the light quantum flux density of the conventional white light is 80 mu mol.m^-2·s^-1The illumination time is 14h/d, and the photon flux density of low-dose UV-B illumination is 3 mu mol.m^-2·s^-1The illumination time is 3h/d, and the ambient temperature is 20-24 ℃.

During the test, the rosette diameter was counted every 7 days, and the chlorophyll content was measured on day 14.

After the green stem vegetables and the four-nine heart vegetables grow under the 2 conditions for the same days, the shapes of the green stem vegetables and the four-nine heart vegetables are observed, and the green stem vegetables and the four-nine heart vegetables grow slowly and have smaller leaves only under the conventional white light irradiation; when irradiated with conventional white light plus low dose UV-B light, green stem vegetables and four-nine heart vegetables grow faster with larger leaves (fig. 2).

First, the sizes of the green stem vegetable and the sijiu vegetable rosette were counted. The result shows that under the conventional white light condition, the rosette diameters of the green stem vegetable and the four-nine heart vegetable are about 20mm, and the growth is slow along with the increase of time; under the condition of conventional white light and low-dose UV-B illumination, the green stalk vegetable and the four-nine flowering cabbage grow faster, the diameter of the rosette increases gradually with the increase of the growth time, and the diameter of the rosette exceeds 40mm after 21 days of growth (figure 3). This result demonstrates that increasing low dose UV-B light can promote the growth of green stem vegetables and leaf of the four-nine heart vegetable.

Secondly, the chlorophyll content in the leaf of the stem vegetable and the leaf of the four nine vegetable heart is detected under the two conditions. The results show a significant increase in chlorophyll content in the leaves of the green stem vegetable and the leaf of the nineteen-cabbages under conventional white light + low dose UV-B illumination conditions (figure 4). This result demonstrates that increased low dose UV-B illumination can induce accumulation of chlorophyll in the leaves of the green stem vegetables and the leaf of the heart of the nineteen vegetables.

In conclusion, the technical scheme of the invention has a remarkable promoting effect by increasing the low-dose UV-B light irradiation, effectively improves the growth and chlorophyll accumulation of seedlings of green stem vegetables and Chinese cabbage heart, and has outstanding advantages. The invention utilizes an optimized illumination method to promote the growth of seedlings of green stem vegetables and four-nine heart vegetables, shorten the production period and reduce the energy consumption. Compared with the traditional illumination mode, the cultivation light environment with the conventional white light and low-dose UV-B illumination provided by the invention can promote the growth of green stem vegetables and leaves of the leaf of the Chinese cabbage and the leaf of the Chinese cabbage and the chlorophyll accumulation, and has good implementation prospect.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are also included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims

1. A light culture method for promoting green stalk vegetable and four nine heart vegetables to increase green is characterized by comprising the following specific steps:

during the growth period of the green stem vegetable and the seedling of the four-nine flowering cabbage, irradiating the green stem vegetable and the seedling of the four-nine flowering cabbage by adopting an artificial light source, wherein the light supply mode of the artificial light source is a light supply mode of conventional white light plus low-dose UV-B illumination;

the light supply mode of the conventional white light and the low-dose UV-B illumination is as follows: in a day-night period of 24h, the irradiation time of the conventional white light is 14h, and the conventional white light is irradiated simultaneously with low-dose UV-B light for 3 h;

the conventional white light has a photon flux density of 80 μmol. m^-2·s^-1(ii) a The irradiation time of the conventional white light is selected from 08: 00-22: 00; the time for irradiating the UV-B light is selected from 11: 00-14: 00;

the light quantum flux density of the low-dose UV-B illumination is 3 mu mol.m^-2·s^-1(ii) a The wavelength of the low-dose UV-B light is 280-320 nm;

the illumination culture method is beneficial to the growth of the leaves of seedlings of green stem vegetables and heart of four-nine cabbages, the green leaves and the chlorophyll accumulation.