CN105850679B - A kind of tobacco seedling method increasing UV-B irradiation - Google Patents
A kind of tobacco seedling method increasing UV-B irradiation Download PDFInfo
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- CN105850679B CN105850679B CN201610212968.XA CN201610212968A CN105850679B CN 105850679 B CN105850679 B CN 105850679B CN 201610212968 A CN201610212968 A CN 201610212968A CN 105850679 B CN105850679 B CN 105850679B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
The present invention relates to a kind of tobacco seedling methods of increase UV-B irradiation.It the described method comprises the following steps: tobacco floating seed rearing: carrying out tobacco floating seed rearing work according to conventional floating seedlings operating instruction；UV-B irradiation: up to seedling stage, every morning 9:00 to the UV-B irradiation for carrying out 10min/d between 10:00 since the tobacco seedlings grand cross phase；The fluorescent tube of UV-B lamp is horizontally suspended on seedlings nursing plate, and with the increase of tobacco seedlings height during treatment with irradiation, and it is about 100 μ W/cm that dynamic, which adjusts position lamp and tobacco seedlings spacing is made to be greater than 30cm and UV-B intensity,2.This method is irradiated processing to tobacco seedlings using UV-B, can moderate control tobacco seedlings form, improve tobacco seedlings plant in certain important resistance substances content, especially suitable for cultivate tobacco floating seed rearing.
Description
Technical field
The present invention relates to a kind of tobacco seedling method, in particular to a kind of tobacco seedling method for increasing UV-B irradiation.
Background technique
Ultraviolet light (Ultraviolet, UV) include short wave ultraviolet light (UV-C, 200-280nm), medium wave ultraviolet light (UV-B,
280-320nm) and long wave ultraviolet light, in, shortwave UV plays the role of disinfection and induction plant generates a variety of defense reactions, appropriate
Ultraviolet light is conducive to crop disease prevention and improves quality.The tobacco seedling phase can cause because of blocking for plastic film with meteorological reason
Uitraviolet intensity reduces in seedling growth greenhouse, and tobacco seedlings is easily made to form the very thin weak seedling of excessive growth.UV is applied to tomato, peppery by existing research person
Nurturing staff in the nursery of the crops such as green pepper and cucumber, and improve the quality of solanberry greengrocery in industrialized agriculture.There is not yet by UV
The report for being applied to tobacco nurturing staff, improving seedling quality.Tobacco is a multilevel complicated tune to the UV response irradiated
Control reaction, is related to the adjusting of physiological-biochemical level, the regulation of adaptation and the gene expression of morphosis.The present invention is by from shape
Influence of the UV-B (parallel control is made with UV-C) to tobacco seedlings is analyzed in state and physiological and biochemical index, it is desirable to provide one kind is suitable for
Cultivate the UV processing method of tobacco strong sprout, Lai Fengfu tobacco strong sprout technology.
Summary of the invention
Seedling quality is improved suitable for tobacco floating seed rearing work the technical problem to be solved by the present invention is to determine a kind of
UV-B illuminating method.
In order to solve the above technical problems, this increase UV-B irradiation tobacco seedling method the following steps are included:
(1) tobacco floating seed rearing: tobacco floating seed rearing work is carried out according to conventional floating seedlings operating instruction；
(2) UV irradiates: up to seedling since the tobacco seedlings grand cross phase, daily in morning 9:00 to carrying out UV between 10:00
The fluorescent tube of UV-C lamp (as parallel control) and UV-B lamp is horizontally suspended on seed plate by irradiation, when irradiation respectively, and in photograph
The increase during handling with tobacco seedlings height is penetrated, dynamic adjusts position.
As specific optimization, UV irradiation time described in step (2) is each 10min.
As specific optimization, UV-C lamp wavelength described in step (2) is 253.7nm, power 30W；The UV-B lamp
Wavelength is 297nm, power 40W.
As specific optimization, dynamic described in step (2) adjusts position, specially remains UV lamp apart from tobacco seedlings top
It is irradiated greater than 300mm.
As specific optimization, step (2) further includes being blocked between UV-B lamp and tobacco seedling with preservative film, cooperates lamp
Away from dynamic adjustment position adjust the intensity of UV-B jointly, UV-B type intensitometer is equipped at the UV-B lamp and is irradiated intensity prison
Control, making the UV-B exposure intensity received at tobacco seedlings apical position is about 100 μ W/cm2。
By control experiment it can be found that Enhanced UV-B irradiation can cause plant leaf blade to thicken, contracting stem strengthens bar, and form changes
Become, inhibit seedling excessive growth, excessive UV irradiation can then destroy plant photosynthesis system, inhibit its growth and development.A large amount of repeatabilities are real
In testing, intensity is 100 μ W/cm2UV-B processing to the biomass of tobacco seedlings, maximum leaf is long and wide, the influence of stem thickness is not significant, but
Root long and vane thickness can be increased significantly, reduce the plant height of tobacco seedlings, this is consistent with result of study of the UV-B to cucumber seedling.This
Outside, the last stage of UV-B irradiation can significantly reduce tobacco seedlings chlorophyll content, but obtain to this chlorophyll content after seedling stage
Restore, this constantly grows up with tobacco seedlings, the raising of itself resistance is related；Influence of the irradiation of UV-C to tobacco seedlings form and biomass
Even more than UV-B significantly suppresses the growth of stem thickness and stem length, reduces chlorophyll content, makes the uneven whole thickness of blade face deformity
Increase, the growth of tobacco seedlings of too strong UV-C severe jamming.
The content of malonaldehyde can reflect the degree of damaged membrane wound, and Proline and polyphenols can be protected directly
Cell is from stress damage.Largely studies have shown that UV irradiation will affect malonaldehyde, Proline and the plant of plant cell
The content of total polyphenols.In this test, when UV irradiates 10d, UV processing malonaldehyde is significantly higher than CK, with previous karyotype studies one
It causes；But when to irradiation 30d, the mda contents of three processing are very close to it is strong to illustrate that tobacco seedlings have adapted to such UV irradiation
Stress, makes Lipid peroxidation metabolism be eased caused by degree.UV irradiation is holding the Proline of tobacco seedlings and Determination of Polyphenols all
It is continuous to increase, it is consistent with forefathers' research.To when irradiating 30d, the free proline content under UV-B processing is significantly higher than CK and UV-C
Processing.
A kind of tobacco seedling method for increasing UV-B irradiation of the present invention is with intensity for 100 μ W/cm2UV-B to tobacco seedlings carry out
The treatment with irradiation of 10min/d, can moderate control tobacco seedlings form, improve tobacco seedlings plant in certain important resistance substances content,
Especially suitable for cultivating tobacco strong sprout, and there is unobviousness.
Specific embodiment
Embodiment: increase UV-B irradiation tobacco seedling method the following steps are included:
(1) tobacco floating seed rearing: tobacco floating seed rearing work is carried out according to conventional floating seedlings operating instruction；
(2) UV irradiates: up to seedling since the tobacco seedlings grand cross phase, daily in morning 9:00 to carrying out UV between 10:00
Irradiation, the UV irradiation time are each 10min, and the fluorescent tube of UV-B lamp is horizontally suspended on seed plate by when irradiation, described
UV-C lamp wavelength be 253.7nm, power 30W；The UV-B lamp wavelength is 297nm, power 40W, and at irradiation
With the increase of tobacco seedlings height during reason, dynamic adjusts position, specially remain UV lamp apart from tobacco seedlings top be greater than 300mm into
Row irradiation.The step can also be blocked between UV-B lamp and tobacco seedling with preservative film, cooperation lamp away from dynamic adjust position
The intensity of UV-B is adjusted jointly, is equipped with UV-B type intensitometer at the UV-B lamp and is carried out the monitoring of UV intensity, makes tobacco seedlings apical position
The UV-B exposure intensity that place receives is about 100 μ W/cm2。
Experiment and control group: when treatment with irradiation, each processing is separated with the black-out cloth of antiultraviolet, has 640 under each processing
Strain tobacco seedlings, in same nursery pond not by UV irradiate tobacco seedlings (CK) and intensity be about 100 μ W/cm2UV-C irradiation as pair
According to.
Morphological index measurement: after the measurement of this morphological index reaches seedling, 10 plants of tobacco seedlings are randomly selected from each processing and are surveyed
Its fixed plant height, stem thickness, root long, mean leaf thickness, maximum leaf leaf area, aerial part do/fresh weight, the fresh and dried ratio of aerial part,
Measurement method measures standard referring to tobacco agronomic trait.
Reason Biochemical Indexes: a sample is respectively taken when irradiating 10d, 20d and 30d after UV, takes three parts of biology every time
Repeated sample；Chlorophyll is measured with spectrophotometry, acid ninhydrine colorimetric method for determining Proline uses thiobarbituricacidα-
Method measures malonaldehyde.The measurement of tobacco leaf total polyphenols is extracted in fresh tobacco leaf with extraction 30min at 40 DEG C of hydrochloric acid solution of 0.1mol/L
Total polyphenols, with the content of forint colorimetric method for determining total polyphenols.
Test the data obtained using EXCEL 2003 and 11.5 software of SPSS it is for statistical analysis, with duncan's new multiple range method into
Row significance test of difference (P < 0.05).
Table 1
Influence of the UV irradiation to tobacco seedlings economical character is as shown in Table 1 above, and the tobacco seedlings under UV processing are in seedling plant height degree and most
CK, UV-B and UV-C processing are substantially less than in great Ye leaf area reduces 20% and 37% than CK in plant height respectively, at two
Reason has dropped 17% and 50% than CK respectively in maximum leaf leaf area.Tobacco seedlings root long ratio CK difference under UV-B and UV-C processing
About 33% and about 38% are increased, vane thickness ratio CK increases about 33%.UV-C processing under tobacco seedlings aerial part dry weight,
Fresh weight all significantly reduces, but its is fresh and dried more similar than the variation handled to UV-B, both greater than CK；This shows that UV handles lower seedling
Middle Free water ratio is lower.Variation on these morphological indexs is conducive to the UV irradiation environment of tobacco seedlings reply higher-strength.Work as cigarette
When seedling is irradiated by the UV-B and UV-C of higher-strength, the decline of plant height and leaf area is beneficial to reduce the face radiated by UV
Product.This change can slow down competition on seed plate between strain, conducive to the leaf-cutting workload reduced when cultivating tobacco seedlings.UV irradiation is lower to plant
Object blade thickness is mainly shown as the increase of blade palisade tissue thickness, this can reduce the penetration capacity of UV, enhance the resistance to of blade
By ability.Though root system does not receive UV irradiation directly, the relevance between UV intensity is also shown, this is influenced in seedling with UV
Source plant hormone and then to influence its root growth related.By finding that CK maximum leaf is wide ellipse, UV to blade face morphologic observation
Maximum leaf under processing is oblong.It has been observed that the maximum leaf under UV-C processing has apparent leaf rolling, bumps not
The phenomenon that putting down, burning, display tobacco seedlings are difficult to adapt to long-term 100 μ W/cm2UV-C irradiation, by irreversible injury.
Table 2
Above-mentioned table 2 is the changes of contents of tobacco seedlings chlorophyll after UV handles 10d and 30d.All in all, the leaf of all tobacco seedlings
Chlorophyll contents are all the extensions with nursery stage and increase；Compared with CK it, UV-B and UV-C irradiation 10d after the tobacco seedlings middle period
The content of green element a and Chlorophyll is decreased significantly, and the chlorophyll content under UV-C processing is substantially less than UV-B processing.UV
After irradiating 30d, UV-B processing and CK be not significant in the difference of chlorophyll a, chlorophyll b and Chlorophyll content；But at UV-C
The content of chlorophyll a and Chlorophyll under reason is still significantly lower than CK, and the difference of chlorophyll b is not significant.It has passed through 100 μ of 30d
W/cm2UV-B irradiates, and the recovery of adaptability has been shown on tobacco seedlings total chlorophyll content, but under the UV-C irradiation of equality strength
But still lower than CK 13%, this shows that UV-C is greater than the effect of UV-B to the inhibition of chlorophyll or damaging action, and UV-B processing is more suitable
For tobacco seedling.The content all lower than CK about 21% of tobacco seedlings the Determination of Chlorophyll a and b of 10d under UV-C processing；Handle 30d
When, the content and CK difference of tobacco seedlings chlorophyll b be not significant, but still has 15% significant difference between Chlorophyll-a Content and CK, can
See that chlorophyll a is more vulnerable to the influence of UV-C than chlorophyll b.
Above embodiment, which is intended to illustrate the present invention, to be realized or use for professional and technical personnel in the field, to above-mentioned
Embodiment, which is modified, will be readily apparent to those skilled in the art, therefore the present invention includes but is not limited to
Above embodiment, it is any to meet the claims or specification description, meet with principles disclosed herein and novelty,
The method of inventive features, technique, product, fall within the scope of protection of the present invention.
Claims (3)
1. a kind of tobacco seedling method for increasing UV-B irradiation, it is characterized in that: the described method comprises the following steps:
Step (1) --- tobacco floating seed rearing: tobacco floating seed rearing work is carried out according to conventional floating seedlings operating instruction；
Step (2) --- UV irradiation: up to seedling since the tobacco seedlings grand cross phase, daily in morning 9:00 between 10:00 into
The fluorescent tube of UV-B lamp is horizontally suspended on seed plate by row UV irradiation, irradiation time 10min, when irradiation respectively, and in irradiation
With the increase of tobacco seedlings height during processing, dynamic adjusts position；
Blocked between the UV-B lamp and tobacco seedling with preservative film, cooperation lamp away from dynamic adjustment position adjust UV-B jointly
Intensity, UV-B type intensitometer is equipped at the UV-B lamp and carries out the monitoring of UV intensity, makes the UV-B received at tobacco seedlings apical position
Exposure intensity is about 100 μ W/cm2。
2. the tobacco seedling method according to claim 1 for increasing UV-B irradiation, it is characterized in that: UV- described in step (2)
B lamp wavelength is 297nm, power 40W.
3. the tobacco seedling method according to claim 1 for increasing UV-B irradiation, it is characterized in that: being moved described in step (2)
State adjusts position, specially remains that UV lamp is irradiated apart from tobacco seedlings top greater than 300mm.
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| CN108266704A (en) * | 2018-01-18 | 2018-07-10 | 电子科技大学 | A kind of pc-LEDs plant growth lamps based on upper conversion ultraviolet fluorescence powder |
| CN108626592B (en) * | 2018-07-10 | 2024-01-23 | 横店集团得邦照明股份有限公司 | Plant lamp based on UVB ultraviolet bacteriostasis and implementation method thereof |
| AU2019376891A1 (en) * | 2018-11-09 | 2021-06-17 | Biolumic Limited | UV-B induced plant pathogen resistance |
| CN111418380B (en) * | 2020-04-15 | 2022-07-19 | 厦门大学 | Illumination culture method for promoting green stalk vegetable and Chinese cabbage heart to increase green |
| CN113711880A (en) * | 2021-09-16 | 2021-11-30 | 湖南省烟草公司衡阳市公司常宁市分公司 | Method for transplanting tobacco in floating seedling raising and plant pulling under film |
| CN115053713A (en) * | 2022-07-18 | 2022-09-16 | 贵州省烟草科学研究院 | Method for inducing tobacco seedling to resist myzus persicae through UV-B irradiation |
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