CN105850679A - Tobacco seedling growing method by increasing UV-B irradiation - Google Patents
Tobacco seedling growing method by increasing UV-B irradiation Download PDFInfo
- Publication number
- CN105850679A CN105850679A CN201610212968.XA CN201610212968A CN105850679A CN 105850679 A CN105850679 A CN 105850679A CN 201610212968 A CN201610212968 A CN 201610212968A CN 105850679 A CN105850679 A CN 105850679A
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- tobacco
- irradiation
- seedling
- seedlings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
Abstract
The invention relates to a tobacco seedling growing method by increasing UV-B irradiation. The method includes the following steps of floating tobacco seedling raising: performing floating tobacco seedling raising according to a conventional floating tobacco seedling raising operation procedure; UV-B irradiation: performing 10min/d UV-B irradiation at the time between 9:00am and 10:00 am every day from the grand cross period of tobacco seedlings to the seedling development period; horizontally hanging lamp tubes of UV-B lamps on seedling nursery trays, and dynamically adjusting the positions of the lamp tubes with increase of the height of the tobacco seedlings in the irradiation treatment period to enable the space between the lamps and the tobacco seedlings to be larger than 30cm and UV-B strength to be around 100 microwatts/cm<2>. The UV-B is utilized to perform irradiation treatment on the tobacco seedlings, shapes of the tobacco seedlings can be controlled properly, content of some important stress resistant substances in the tobacco seedling plants is increased, and the method is particularly suitable for culture of floating tobacco seedling raising.
Description
Technical field
The present invention relates to a kind of tobacco seedling method, increase, particularly to a kind of, the tobacco seedling that UV-B irradiates
Method.
Background technology
Ultraviolet light (Ultraviolet, UV) comprises short wave ultraviolet light (UV-C, 200-280nm), medium wave purple
Outer light (UV-B, 280-320nm) and long wave ultraviolet light, in, shortwave UV have sterilization and induction plant produce
The effect of raw multiple defense reaction, appropriate ultraviolet light irradiates and is conducive to crop disease prevention and improves quality.Nicotiana tabacum L.
Nursery stage can cause uitraviolet intensity in seedling growth greenhouse to reduce because of blocking of plastic sheeting with meteorological reason, easily makes cigarette
Seedling forms the very thin weak Seedling of excessive growth.UV is applied to the crops such as Fructus Lycopersici esculenti, Fructus Capsici and Fructus Cucumidis sativi by existing researcher
Nurturing staff in nursery, and improve the quality of solanberry greengrocery in industrialized agriculture.There is not yet and UV is applied
To Nicotiana tabacum L. nurturing staff, the report of raising seedling quality.Nicotiana tabacum L. to the response that UV irradiates be one multilevel
Complicated regulation and control reaction, relate to the regulation of physiological-biochemical level, the adaptation of morphosis and gene expression
Regulation and control.(it is parallel right to make with UV-C by analyzing UV-B from form with physiological and biochemical index for the present invention
According to) impact on tobacco seedlings, it is desirable to provide a kind of UV processing method being applicable to cultivate Nicotiana tabacum L. strong sprout, come rich
Rich Nicotiana tabacum L. technology in strong sprout.
Summary of the invention
The technical problem to be solved in the present invention determines that one improves cigarette be applicable to tobacco floating seed rearing works
The UV-B illuminating method of Seedling quality.
For solving above-mentioned technical problem, the tobacco seedling method that this increase UV-B irradiates comprises the following steps:
(1) tobacco floating seed rearing: carry out tobacco floating seed rearing work according to conventional floating seedlings rule of operation；
(2) UV irradiates: starting from the tobacco seedlings grand cross phase until seedling, every day is in 9:00 to 10 in morning:
UV irradiation is carried out, respectively using UV-C lamp (as parallel control) and UV-B lamp during irradiation between 00
Fluorescent tube is flatly suspended in Miao Panshang, and with the increase of tobacco seedlings height during treatment with irradiation, dynamically adjusts position
Put.
Optimizing as concrete, the UV irradiation time described in step (2) is each 10min.
Optimizing as concrete, the UV-C lamp wavelength described in step (2) is 253.7nm, power is 30W；
Described UV-B lamp wavelength is 297nm, power is 40W.
Optimize as concrete, described in step (2), dynamically adjust position, specially remain UV lamp
Distance tobacco seedlings top is irradiated more than 300mm.
Optimizing as concrete, step (2) is additionally included between UV-B lamp and tobacco seedling and carries out with preservative film
Block, coordinate lamp away from the position that dynamically adjusts jointly regulate the intensity of UV-B, be provided with at described UV-B lamp
UV-B type intensitometer is irradiated intensity monitoring, makes the UV-B exposure rate accepted at tobacco seedlings apical position
It is about 100 μ W/cm2。
By control experiment it is found that Enhanced UV-B irradiation can cause plant leaf blade to thicken, contracting stem strengthens bar,
The change of form, suppresses Seedling excessive growth, and excessive UV irradiates then can destroy plant photosynthesis system, suppresses it raw
Long growth.In a large amount of repeatable experiments, intensity is 100 μ W/cm2UV-B process biology to tobacco seedlings
Amount, maximum leaf length and width, the impact that stem is thick are notable, but can significantly increase root length and vane thickness, fall
The plant height of low tobacco seedlings, this is consistent to the result of study of cucumber seedling with UV-B.Additionally, what UV-B irradiated
Last stage can significantly reduce tobacco seedlings chlorophyll content, but is recovered to this chlorophyll content of seedling after date,
This constantly grows up with tobacco seedlings, the raising of self resistance is relevant；The irradiation of UV-C is to tobacco seedlings form and biology
The impact of amount is even more than UV-B, significantly suppress that stem is thick and the growth of stem length, reduction chlorophyll content, makes
The blade face uneven whole thickness of deformity increases, the growth of tobacco seedlings of too strong UV-C severe jamming.
The content of malonaldehyde can reflect that the degree that damaged membrane is hindered, Proline and polyphenols can be straight
Connect and protect cells from stress damage.Substantial amounts of research shows, UV irradiation can affect the third the two of plant cell
Aldehyde, Proline and the content of plant total polyphenols.In this test, when UV irradiates 10d, at UV
Reason malonaldehyde is significantly higher than CK, consistent with previous karyotype studies；But during to irradiation 30d, three process
Mda content very close to, illustrate that what tobacco seedlings adapted to that this kind of UV exposure rate cause coerces, make
Lipid peroxidation metabolism is eased.UV irradiates and makes the Proline of tobacco seedlings and Determination of Polyphenols persistently increase
Add, study with forefathers consistent.To when irradiating 30d, the free proline content under UV-B processes is significantly high
In CK and UV-C process.
The tobacco seedling method that a kind of UV-B of increasing of the present invention irradiates is 100 μ W/cm with intensity2UV-B
Tobacco seedlings is carried out the treatment with irradiation of 10min/d, can moderate control tobacco seedlings form, improve some in tobacco seedlings plant
The content of important resistance material, is particularly well-suited to cultivate Nicotiana tabacum L. strong sprout, and has unobviousness.
Detailed description of the invention
Embodiment: the tobacco seedling method increasing UV-B irradiation comprises the following steps:
(1) tobacco floating seed rearing: carry out tobacco floating seed rearing work according to conventional floating seedlings rule of operation；
(2) UV irradiates: starting from the tobacco seedlings grand cross phase until seedling, every day is in 9:00 to 10 in morning:
Carrying out UV irradiation between 00, described UV irradiation time is each 10min, by UV-B lamp during irradiation
Fluorescent tube be flatly suspended in Miao Panshang, described UV-C lamp wavelength is 253.7nm, power is 30W；
Described UV-B lamp wavelength is 297nm, power is 40W, and with tobacco seedlings height during treatment with irradiation
Increase, dynamically adjust position, specially remain that UV lamp distance tobacco seedlings top is carried out more than 300mm
Irradiate.This step also can be blocked with preservative film between UV-B lamp and tobacco seedling, coordinate lamp away from dynamic
State adjusts position and jointly regulates the intensity of UV-B, is provided with UV-B type intensitometer and carries out at described UV-B lamp
UV intensity monitors, and makes the UV-B exposure rate accepted at tobacco seedlings apical position be about 100 μ W/cm2。
Experiment and matched group: during treatment with irradiation, separate each process, each process with the gobo of anti-ultraviolet
Under have 640 strain tobacco seedlings, tobacco seedlings (CK) and intensity not irradiated by UV in same nursery pond are about
100μW/cm2UV-C irradiate as comparison.
Morphological index measures: this morphological index measures after reaching seedling, randomly selects 10 from each process
Strain tobacco seedlings measures that its plant height, stem be thick, root length, mean leaf thickness, maximum leaf leaf area, aerial parts are done
The fresh and dried ratio of/fresh weight, aerial parts, measuring method is with reference to Nicotiana tabacum L. agronomic trait bioassay standard.
Reason Biochemical Indexes: respectively take a sample, every time when irradiating 10d, 20d and 30d after UV
Take three parts biology repeated sample；Using spectrophotometry chlorophyll, acid ninhydrine colorimetric method for determining is swum
From proline, measure malonaldehyde by thiobarbituricacidα-method.The salt of Nicotiana tabacum L. total polyphenols mensuration 0.1mol/L
Extract 30min at acid solution 40 DEG C and extract the total polyphenols in fresh tobacco leaf, with forint colorimetric method for determining total polyphenols
Content.
Test the data obtained uses EXCEL 2003 and SPSS 11.5 software to carry out statistical analysis, with new multiple
Range method carries out significance test of difference (P < 0.05).
Table 1
UV irradiates the impact on tobacco seedlings economical character as shown in Table 1 above, and the tobacco seedlings under UV process is at Seedling
All it is substantially less than CK, UV-B and UV-C process on plant height degree and maximum leaf leaf area on plant height, compares CK
Reducing 20% and 37% respectively, two process have dropped 17% He than CK in maximum leaf leaf area respectively
50%.Tobacco seedlings root length under UV-B and UV-C processes adds about 33% and about 38% respectively than CK,
Vane thickness adds about 33% than CK.Under UV-C processes, the dry weight of tobacco seedlings aerial parts, fresh weight are all
It is to significantly reduce, but it is fresh and dried than similar to the change that UV-B processes, both greater than CK；This shows
It is relatively low that UV processes Free water ratio in lower Seedling.Change on these morphological indexs is conducive to tobacco seedlings reply higher
The UV of intensity irradiates environment.When tobacco seedlings by higher-strength UV-B and UV-C irradiate time, plant height and
The decline of leaf area is beneficial to reduce the area radiated by UV.This change can slow down on Seedling dish between strain
Competition, be beneficial to reduce cultivate tobacco seedlings time leaf-cutting workload.UV irradiates lower plant leaf blade and thickens, mainly
Showing as the increase of blade palisade tissue thickness, this can reduce the penetration capacity of UV, strengthen the tolerance of blade
Ability.Irradiate though root system the most directly accepts UV, but also show and relatedness between UV intensity, this
Affect seedling Endogenous hormones with UV and then to affect its root growth relevant.By to blade face Appearance View
Examining discovery CK maximum leaf oval for width, the maximum leaf under UV processes is oblong.It has been observed that
UV-C process lower maximum leaf have obvious leaf rolling, uneven, have the phenomenon burnt, display cigarette
Seedling is difficult in adapt to 100 long-term μ W/cm2UV-C and irradiates, by irreversible injury.
Table 2
Above-mentioned table 2 is tobacco seedlings chlorophyllous changes of contents after UV processes 10d and 30d.All in all, institute
The chlorophyll content having tobacco seedlings is all as the prolongation of nursery stage and increases；Compared with CK it, at UV-B and
Tobacco seedlings Determination of Chlorophyll a and the content of Chlorophyll after UV-C irradiation 10d are all decreased significantly, and UV-C process
Under chlorophyll content be substantially less than UV-B process.UV irradiates after 30d, UV-B process with CK chlorophyll a,
The difference of chlorophyll b and Chlorophyll content is the most notable；But the chlorophyll a under UV-C process and Chlorophyll
Content be still significantly lower than CK, the difference of chlorophyll b is the most notable.Have passed through the 100 μ W/cm of 30d2UV-B
Irradiate, tobacco seedlings total chlorophyll content has shown adaptive recovery, but the UV-C of equality strength irradiates
Under still the lowest by 13% than CK, this show UV-C to the effect more than UV-B of chlorophyllous suppression or damaging action,
UV-B process is more suitable for tobacco seedling.Under UV-C processes, the content of tobacco seedlings Determination of Chlorophyll a and b of 10d is all
Lower by about 21% than CK；When processing 30d, the content of tobacco seedlings chlorophyll b and CK difference is not the most notable, but leaf is green
The significant difference of 15% is still had, it is seen that chlorophyll a is more vulnerable to UV-C's than chlorophyll b between element a content and CK
Impact.
It can be that professional and technical personnel in the field realize or use that above-mentioned embodiment is intended to illustrate the present invention,
Above-mentioned embodiment is modified and will be apparent from for those skilled in the art, therefore this
Invention includes but not limited to above-mentioned embodiment, any meets the claims or description describes, and meets
With principles disclosed herein and novelty, the method for inventive features, technique, product, each fall within this
Within bright protection domain.
Claims (5)
1. increase the tobacco seedling method that UV-B irradiates, it is characterized in that: described method includes following
Step:
Step (1) tobacco floating seed rearing: carry out tobacco floating according to conventional floating seedlings rule of operation
Nursery works；
Step (2) UV irradiates: starting until seedling from the tobacco seedlings grand cross phase, every day is morning 9:
Carry out UV irradiation between 00 to 10:00, respectively the fluorescent tube of UV-B lamp is flatly suspended in during irradiation
On Seedling dish, and with the increase of tobacco seedlings height during treatment with irradiation, dynamically adjust position.
The tobacco seedling method that increase UV-B the most according to claim 1 irradiates, is characterized in that:
UV irradiation time described in step (2) is each 10min.
The tobacco seedling method that increase UV-B the most according to claim 1 irradiates, is characterized in that:
UV-B lamp wavelength described in step (2) is 297nm, power is 40W.
The tobacco seedling method that increase UV-B the most according to claim 1 irradiates, is characterized in that:
Dynamically adjust position described in step (2), specially remain that UV lamp distance tobacco seedlings top is more than
300mm is irradiated.
The tobacco seedling method that increase UV-B the most according to claim 1 irradiates, is characterized in that:
Step (2) also includes blocking with preservative film between UV-B lamp and tobacco seedling, coordinate lamp away from dynamic
Adjust position and jointly regulate the intensity of UV-B, be provided with UV-B type intensitometer at described UV-B lamp and carry out UV
Intensity monitors, and makes the UV-B exposure rate accepted at tobacco seedlings apical position be about 100 μ W/cm2。
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Cited By (7)
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| CN108266704A (en) * | 2018-01-18 | 2018-07-10 | 电子科技大学 | A kind of pc-LEDs plant growth lamps based on upper conversion ultraviolet fluorescence powder |
| CN108626592A (en) * | 2018-07-10 | 2018-10-09 | 横店集团得邦照明股份有限公司 | It is a kind of based on UVB ultraviolet antibacterial plant lamp and its implementation |
| CN110809398A (en) * | 2017-04-28 | 2020-02-18 | 日亚化学工业株式会社 | Method for increasing phenolic compounds in plants |
| CN111418380A (en) * | 2020-04-15 | 2020-07-17 | 厦门大学 | Illumination culture method for promoting green stalk vegetable and four-nine heart vegetable to increase green |
| CN113271764A (en) * | 2018-11-09 | 2021-08-17 | 拜欧卢米克有限公司 | UV-B induced resistance to plant pathogens |
| CN115053713A (en) * | 2022-07-18 | 2022-09-16 | 贵州省烟草科学研究院 | Method for inducing tobacco seedling to resist myzus persicae through UV-B irradiation |
| CN114830982B (en) * | 2021-09-16 | 2023-06-16 | 湖南省烟草公司衡阳市公司常宁市分公司 | Tobacco floating seedling pulling and transplanting method under film |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110809398A (en) * | 2017-04-28 | 2020-02-18 | 日亚化学工业株式会社 | Method for increasing phenolic compounds in plants |
| CN108266704A (en) * | 2018-01-18 | 2018-07-10 | 电子科技大学 | A kind of pc-LEDs plant growth lamps based on upper conversion ultraviolet fluorescence powder |
| CN108626592A (en) * | 2018-07-10 | 2018-10-09 | 横店集团得邦照明股份有限公司 | It is a kind of based on UVB ultraviolet antibacterial plant lamp and its implementation |
| CN108626592B (en) * | 2018-07-10 | 2024-01-23 | 横店集团得邦照明股份有限公司 | Plant lamp based on UVB ultraviolet bacteriostasis and implementation method thereof |
| CN113271764A (en) * | 2018-11-09 | 2021-08-17 | 拜欧卢米克有限公司 | UV-B induced resistance to plant pathogens |
| CN111418380A (en) * | 2020-04-15 | 2020-07-17 | 厦门大学 | Illumination culture method for promoting green stalk vegetable and four-nine heart vegetable to increase green |
| CN114830982B (en) * | 2021-09-16 | 2023-06-16 | 湖南省烟草公司衡阳市公司常宁市分公司 | Tobacco floating seedling pulling and transplanting method under film |
| CN115053713A (en) * | 2022-07-18 | 2022-09-16 | 贵州省烟草科学研究院 | Method for inducing tobacco seedling to resist myzus persicae through UV-B irradiation |
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