CN107396728A - A kind of cuttage and seedling culture method for improving ginkgo frost resistance - Google Patents
A kind of cuttage and seedling culture method for improving ginkgo frost resistance Download PDFInfo
- Publication number
- CN107396728A CN107396728A CN201710629099.5A CN201710629099A CN107396728A CN 107396728 A CN107396728 A CN 107396728A CN 201710629099 A CN201710629099 A CN 201710629099A CN 107396728 A CN107396728 A CN 107396728A
- Authority
- CN
- China
- Prior art keywords
- cutting
- cuttage
- frost resistance
- illumination
- ginkgo
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0299—Handling or transporting of soil blocks or seedlings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/14—Measures for saving energy, e.g. in green houses
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Soil Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Cultivation Of Plants (AREA)
Abstract
The present invention relates to a kind of cuttage and seedling culture method for improving ginkgo frost resistance, comprise the following steps：（1）The clip of cutting；（2）Cutting single treatment；（3）Cutting after-treatment；（4）After above-mentioned processing, cutting can be transplanted to outdoor.A kind of cuttage and seedling culture method of raising ginkgo frost resistance provided by the invention is simple to operate, use the inventive method, ginkgo tree cuttage seedling freezing tolerance is substantially increased, the survival rate of passing the winter of ginkgo tree cuttage seedling is promoted to more than 89.5% by the 71.8% of routine, significant effect.
Description
Technical field
The invention belongs to planting technology field, and in particular to a kind of cuttage and seedling culture method for improving ginkgo frost resistance.
Background technology
Ginkgo, it is Ginkgoaceae, Ginkgo deciduous tree.Ginkgo is fallen leaves megaphanerophyte, and the diameter of a cross-section of a tree trunk 1.3 meters above the ground up to 4 meters, bloom by April, 10
The moon is ripe, and the long stalk of seed tool, sagging, normal is ellipse, long inverted ovoid, oval or nearly spheroidal.Kind of skin and flesh matter, by white powder,
Exosper meat, yellow or orange-yellow when ripe.Ginkgo growth is slower, and the life-span is extremely long, is the venerable old man or lady in tree, have view and admire,
The value such as economic, medicinal.
Ginkgo cuttage and seedling culture method is single, does not there is the side for coordinating stress to take exercise during cuttage and seedling culture in the prior art
Method.
The content of the invention
In order to solve the above problems, it is an object of the invention to provide a kind of cuttage and seedling culture side for improving ginkgo frost resistance
Method, the rooting rate of cutting is improved, while reduce required time of taking root.
The present invention is achieved through the following technical solutions：
A kind of cuttage and seedling culture method for improving ginkgo frost resistance, comprises the following steps：
（1）The clip of cutting
Excellent maternal plant is selected, clip no disease and pests harm, annual or biennial, rugosity are inserted in 1-2 centimetres of semi-lignified green branch
Bar, retain cutting top 2-3 piece leaves,
（2）Cutting single treatment
By the peeling of cutting end, water planting 3-5 days in 8-12 DEG C of nutrient solution are placed in, it is daily to keep light application time 12-15 hours, illumination
1500-1800lx；Cultivated using the nutrient solution, can be that adaptation transitional function is played in the cultivation of follow-up low temperature, while avoid only
Cool processing rather than the reduction of integrated environment temperature to cutting end, avoids blade tender shoots from causing cutting dead by freeze injury, simultaneously
Increase illumination, ensure the intensity of photosynthesis of cutting, there is provided enough energy, give full expression to cutting frost resistance related gene,
Improve the frost resistance of ginkgo cutting；
（3）Cutting after-treatment
Environment temperature is promoted to 32-35 DEG C, the solution temperature is reduced to 2-4 DEG C, and light application time, illumination illumination are kept not
Become, continue to cultivate cutting 4-6 days, the nurturing period every 1 hour into the nutrient solution in be passed through 0.3A DC currents 60-90
Second；By step（2）Adaptive processes, cutting is adapted to the stress of lower temperature and take exercise, while improve environment temperature, enter
One step lifts the respiration and intensity of photosynthesis of cutting, ensures that cutting obtains sufficient cold acclimation without freeze injury occurs,
Nurturing period applies electric current, can wake up the differentiation of cutting cell of termination, further stimulates the expression of frost resistance related gene, makes
The frost resistance for the seedling cultivated greatly improves；
（4）After above-mentioned processing, cutting can be transplanted to outdoor.
Beneficial effects of the present invention：The present invention is cultivated using the nutrient solution, can be played and be adapted to for the cultivation of follow-up low temperature
The effect of crossing, while only cool to cutting end processing rather than the reduction of integrated environment temperature are avoided, avoid blade tender shoots from being frozen
Evil causes cutting dead, while increases illumination, ensures the intensity of photosynthesis of cutting, there is provided enough energy, make cutting freeze proof
Property related gene give full expression to, improve ginkgo cutting frost resistance；By step（2）Adaptive processes, adapt to cutting
The stress of lower temperature is taken exercise, secondary reduction solution temperature, while improves environment temperature, and the breathing for further lifting cutting is made
With and intensity of photosynthesis, ensure that cutting obtains sufficient cold acclimation and applies electric current without freeze injury, nurturing period occurs, can be with
The differentiation of cutting cell of termination is waken up, the expression of frost resistance related gene is further stimulated, makes the frost resistance of seedling cultivated
Greatly improve.By experiment, using the inventive method, ginkgo tree cuttage seedling freezing tolerance is substantially increased, makes ginkgo cuttage
The survival rate of passing the winter of seedling is promoted to more than 89.5% by the 71.8% of routine, significant effect.
Embodiment
Embodiment 1
A kind of cuttage and seedling culture method for improving ginkgo frost resistance, comprises the following steps：
（1）The clip of cutting
Excellent maternal plant is selected, clip no disease and pests harm, annual or biennial, rugosity are inserted in 1-2 centimetres of semi-lignified green branch
Bar, retain cutting top 2-3 piece leaves,
（2）Cutting single treatment
By the peeling of cutting end, water planting 3 days in 8-12 DEG C of nutrient solution are placed in, daily to keep light application time 12 hours, illumination
1500lx；Cultivated using the nutrient solution, can be that adaptation transitional function is played in the cultivation of follow-up low temperature, while avoid only to cutting
End cooling processing rather than integrated environment temperature reduce, and avoid blade tender shoots from causing cutting dead by freeze injury, while increase light
According to ensureing the intensity of photosynthesis of cutting, there is provided enough energy, give full expression to cutting frost resistance related gene, improve silver
The frost resistance of apricot cutting；
（3）Cutting after-treatment
Environment temperature is promoted to 32-35 DEG C, the solution temperature is reduced to 2-4 DEG C, and light application time, illumination illumination are kept not
Become, continue to cultivate cutting 4 days, the nurturing period every 1 hour into the nutrient solution in be passed through 0.3A DC currents 60 seconds；By
Step（2）Adaptive processes, cutting is adapted to the stress of lower temperature and take exercise, while improve environment temperature, further carry
The respiration and intensity of photosynthesis of cutting are risen, ensures that cutting obtains sufficient cold acclimation without freeze injury, nurturing period occurs
Between apply electric current, the differentiation of cutting cell of termination can be waken up, further stimulate frost resistance related gene expression, make to cultivate
The frost resistance of seedling greatly improve；
（4）After above-mentioned processing, cutting can be transplanted to outdoor.
Embodiment 2
A kind of cuttage and seedling culture method for improving ginkgo frost resistance, comprises the following steps：
（1）The clip of cutting
Excellent maternal plant is selected, clip no disease and pests harm, annual or biennial, rugosity are inserted in 1-2 centimetres of semi-lignified green branch
Bar, retain cutting top 2-3 piece leaves,
（2）Cutting single treatment
By the peeling of cutting end, water planting 5 days in 8-12 DEG C of nutrient solution are placed in, daily to keep light application time 15 hours, illumination
1800lx；Cultivated using the nutrient solution, can be that adaptation transitional function is played in the cultivation of follow-up low temperature, while avoid only to cutting
End cooling processing rather than integrated environment temperature reduce, and avoid blade tender shoots from causing cutting dead by freeze injury, while increase light
According to ensureing the intensity of photosynthesis of cutting, there is provided enough energy, give full expression to cutting frost resistance related gene, improve silver
The frost resistance of apricot cutting；
（3）Cutting after-treatment
Environment temperature is promoted to 32-35 DEG C, the solution temperature is reduced to 2-4 DEG C, and light application time, illumination illumination are kept not
Become, continue to cultivate cutting 6 days, the nurturing period every 1 hour into the nutrient solution in be passed through 0.3A DC currents 90 seconds；By
Step（2）Adaptive processes, cutting is adapted to the stress of lower temperature and take exercise, while improve environment temperature, further carry
The respiration and intensity of photosynthesis of cutting are risen, ensures that cutting obtains sufficient cold acclimation without freeze injury, nurturing period occurs
Between apply electric current, the differentiation of cutting cell of termination can be waken up, further stimulate frost resistance related gene expression, make to cultivate
The frost resistance of seedling greatly improve；
（4）After above-mentioned processing, cutting can be transplanted to outdoor.
Comparative example 1
This comparative example 1 compared with Example 1, without step（1）The operation, method and step all same in addition.
Comparative example 2
This comparative example 2 compared with Example 2, without using step（2）The operation, method and step all same in addition.
Control group
Existing ginkgo cuttage and seedling culture method.
Ensure that the cutting for being derived from same maternal plant carries out cuttage and seedling culture using above-mentioned 5 kinds of methods respectively, then after contrast transplanting
That passes the winter survives number, can reflect the frost resistance of seedling, by many experiments, as a result such as table 1：：
Table 1
| Cutting number | Pass the winter and survive number | Survival rate（%） | |
| Embodiment 1 | 400 | 358 | 89.5 |
| Embodiment 2 | 400 | 364 | 91.0 |
| Comparative example 1 | 400 | 315 | 78.8 |
| Comparative example 2 | 400 | 303 | 75.8 |
| Control group | 400 | 287 | 71.8 |
As shown in Table 1, using the inventive method, ginkgo tree cuttage seedling freezing tolerance is substantially increased, makes ginkgo tree cuttage seedling
Survival rate of passing the winter is promoted to more than 89.5% by the 71.8% of routine, significant effect.
Claims (3)
1. a kind of cuttage and seedling culture method for improving ginkgo frost resistance, it is characterised in that comprise the following steps：
（1）The clip of cutting
Excellent maternal plant is selected, clip no disease and pests harm, annual or biennial, rugosity are inserted in 1-2 centimetres of semi-lignified green branch
Bar, retain cutting top 2-3 piece leaves,
（2）Cutting single treatment
By the peeling of cutting end, water planting 3-5 days in 8-12 DEG C of nutrient solution are placed in, it is daily to keep light application time 12-15 hours, illumination
1500-1800lx；
（3）Cutting after-treatment
Environment temperature is promoted to 32-35 DEG C, the solution temperature is reduced to 2-4 DEG C, and light application time, illumination illumination are kept not
Become, continue to cultivate cutting 4-6 days, the nurturing period every 1 hour into the nutrient solution in be passed through 0.3A DC currents 60-90
Second；
（4）After above-mentioned processing, cutting can be transplanted to outdoor.
A kind of 2. cuttage and seedling culture method for improving ginkgo frost resistance according to claim 1, it is characterised in that step
（2）The cutting single treatment, method for optimizing are:By the peeling of cutting end, water planting 5 days in 8-12 DEG C of nutrient solution are placed in, daily
Keep light application time 15 hours, illumination 1800lx.
A kind of 3. cuttage and seedling culture method for improving ginkgo frost resistance according to claim 1, it is characterised in that step
（3）The cutting after-treatment, method for optimizing are:Environment temperature is promoted to 32-35 DEG C, the solution temperature is reduced to 2-
4 DEG C, light application time, illumination illumination keep constant, continue to cultivate cutting 6 days, the nurturing period is every 1 hour into the nutrient solution
In be passed through 0.3A DC currents 90 seconds.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710629099.5A CN107396728A (en) | 2017-07-28 | 2017-07-28 | A kind of cuttage and seedling culture method for improving ginkgo frost resistance |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710629099.5A CN107396728A (en) | 2017-07-28 | 2017-07-28 | A kind of cuttage and seedling culture method for improving ginkgo frost resistance |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107396728A true CN107396728A (en) | 2017-11-28 |
Family
ID=60401471
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710629099.5A Pending CN107396728A (en) | 2017-07-28 | 2017-07-28 | A kind of cuttage and seedling culture method for improving ginkgo frost resistance |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107396728A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109906787A (en) * | 2019-01-06 | 2019-06-21 | 山东农业大学 | A kind of method that Cryogenic Temperature Swing tempers raising chrysanthemum cold resistance |
| CN111436323A (en) * | 2020-04-02 | 2020-07-24 | 吉林市林业科学研究院(吉林市林业技术推广站) | Cultivation method for improving cold resistance of roses |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1806513A (en) * | 2006-01-24 | 2006-07-26 | 王录顺 | Cold-resistant cypress breeding method |
| CN102057871A (en) * | 2010-11-08 | 2011-05-18 | 罗先财 | Method for breeding cold-resistant eucalypt Xiang eucalypt No.1 variety suitable for north latitude 26 to 30 degrees |
| CN106561214A (en) * | 2016-10-29 | 2017-04-19 | 蚌埠市涂山绿园蔬菜科研专业合作社 | Cutting seedling growing method for improving freezing resistance of pomegranate |
| CN106688769A (en) * | 2016-11-18 | 2017-05-24 | 蚌埠市金牛湾农业科技发展有限公司 | Cutting treatment method for cutting seedling of maidenhair trees |
-
2017
- 2017-07-28 CN CN201710629099.5A patent/CN107396728A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1806513A (en) * | 2006-01-24 | 2006-07-26 | 王录顺 | Cold-resistant cypress breeding method |
| CN102057871A (en) * | 2010-11-08 | 2011-05-18 | 罗先财 | Method for breeding cold-resistant eucalypt Xiang eucalypt No.1 variety suitable for north latitude 26 to 30 degrees |
| CN106561214A (en) * | 2016-10-29 | 2017-04-19 | 蚌埠市涂山绿园蔬菜科研专业合作社 | Cutting seedling growing method for improving freezing resistance of pomegranate |
| CN106688769A (en) * | 2016-11-18 | 2017-05-24 | 蚌埠市金牛湾农业科技发展有限公司 | Cutting treatment method for cutting seedling of maidenhair trees |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109906787A (en) * | 2019-01-06 | 2019-06-21 | 山东农业大学 | A kind of method that Cryogenic Temperature Swing tempers raising chrysanthemum cold resistance |
| CN111436323A (en) * | 2020-04-02 | 2020-07-24 | 吉林市林业科学研究院(吉林市林业技术推广站) | Cultivation method for improving cold resistance of roses |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106386185A (en) | High-yield early-fruiting cultivation method for mulberry | |
| CN105684811B (en) | A kind of method of pineapple Sakyamuni teletostage fruit production | |
| CN106613718A (en) | Cultivation method for grape cutting seedlings with high cold resistance | |
| CN106305428B (en) | A kind of elaeagnus mollis bud multiplication and plant regeneration method | |
| CN103404438A (en) | Acer paimatum seed tissue culture method | |
| CN103004595A (en) | Twig cuttage breeding method for ginseng fruit | |
| CN107396728A (en) | A kind of cuttage and seedling culture method for improving ginkgo frost resistance | |
| CN104813938A (en) | Panax notoginseng tissue culture seedling raising method | |
| CN103931496A (en) | Dendrobium flexicaule tissue culture and rapid propagation culture medium as well as tissue culture and rapid propagation method | |
| CN103598097B (en) | The cultural method of virus-free snake gourd plantlet in vitro | |
| CN106613993A (en) | Culture method of tissue culture regeneration seedlings of trifoliate oranges | |
| CN111149702A (en) | Rapid propagation method of root segment induced buds of Korean epimedium herb | |
| CN106386344A (en) | Rejuvenation and fruit bearing method for aging fruit bearing jujube trees | |
| CN105724167A (en) | Control method for pomegranate crack resistance in north of Anhui province | |
| CN105145269A (en) | Efficient seedling cultivating method for leafbuds of Yunshan 2# grape novel variety | |
| KR101439618B1 (en) | A Method for Mass Propagation of Rhododendron Keiskei var. hypoglaucum by Plant Tissue Culture | |
| CN109863928A (en) | One seed pod mulberry cultivation management method | |
| CN106358631A (en) | Method for cultivating snowflake pear tree | |
| CN107896987B (en) | Breeding method of Guangxi wild adinandra nitida tissue culture seedlings | |
| CN104521481A (en) | Strawberry planting method | |
| KR101899140B1 (en) | Method of plant culture for mass propagation of Aralia elata Seem | |
| CN104920221A (en) | In-vitro circular conservation method of potato germplasms | |
| CN105284517A (en) | Plantation and management method of apple trees | |
| CN109329051A (en) | Jasmine quickly breeds the preparation method with culture medium | |
| CN117413698B (en) | Grape cutting method suitable for northwest region |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171128 |
|
| RJ01 | Rejection of invention patent application after publication |