CN106613718A - Cultivation method for grape cutting seedlings with high cold resistance - Google Patents
Cultivation method for grape cutting seedlings with high cold resistance Download PDFInfo
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- CN106613718A CN106613718A CN201611057006.8A CN201611057006A CN106613718A CN 106613718 A CN106613718 A CN 106613718A CN 201611057006 A CN201611057006 A CN 201611057006A CN 106613718 A CN106613718 A CN 106613718A
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- cuttage
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/02—Cultivation of hops or vines
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/14—Measures for saving energy, e.g. in green houses
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- Biodiversity & Conservation Biology (AREA)
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Abstract
The invention discloses a cultivation method for grape cutting seedlings with the high cold resistance, and belongs to the field of planting. The cultivation method includes the following specific steps of (1) cutting selection; (2) soaking treatment; (3) cutting inserting; (4) post-cutting management; (5) seedling transplanting. By means of the cultivation method, the survival rate of grape cutting can be well increased, the cultivated cutting seedlings are high in cold resistance, and the cultivation method is suitable for industrial seedling cultivating production.
Description
Technical field
The invention belongs to field of planting, and in particular to a kind of strong grape cuttage seeding cultivating method of winter resistance.
Background technology
Grape is one of common fruit in people's life, because its nutritive value is abundant, better economic benefit and it is extensive
Plantation.The temperature-resistance characteristic of grapevine seedling is general, needs to carry out artificial protection process, such as mulching straw stalk when generally surviving the winter
Deng, it is clear that increased the labour intensity of artificial growth.The existing method for lifting grape cold resistance has transgenosis to process, low temperature forging
Refining process, radiation modification process etc., transgenosis process is complex, and not with dissemination, the time is maintained in cold acclimation process
It is shorter, do not have persistence, radiation modification processing method is various, and using effect is still waited to improve.Current people educate for grape cuttage
The effective ways for how improving grape cold resistance in seedling are still more deficient.
The content of the invention
The present invention is intended to provide a kind of strong grape cuttage seeding cultivating method of winter resistance, the cuttage seeding for bringing out has good
Cold resistant property.
The present invention is achieved through the following technical solutions：
A kind of strong grape cuttage seeding cultivating method of winter resistance, comprises the steps：
（1）Cuttings is chosen：
1 year raw branch for growing full sturdy, eye, no disease and pests harm, semi-lignified is chosen as skewer fringe, cuttings is intercepted a length of
14-18cm, retains 2 ~ 3 sections, and the otch of skewer fringe lower end will be near section portion or axil portion, and distance is 0.2-0.3cm, its otch
With sharp blade or razor, chamfer by 45° angle, imposed uniformity without examining individual cases disconnected to ensure that otch is smoothed, cuttings is obtained after cutting standby；
（2）Immersion treatment：
By step（1）In the cuttings bottom immersion treatment fluid of gained, it is 32 ~ 35 DEG C that heating keeps processing liquid temp, immersion treatment
Take out after 20 ~ 25min standby；The treatment fluid by following percentage by weight material composition：0.004 ~ 0.006% copper sulphate,
0.005 ~ 0.007% zinc sulfate, 0.003 ~ 0.005% manganese sulfate, 0.008 ~ 0.01% ferrous sulfate, 0.01 ~ 0.013% boric acid,
0.004 ~ 0.006% sodium molybdate, 0.03 ~ 0.05% polyethylene glycol, 0.04 ~ 0.07% fructose, 0.03 ~ 0.06% chitosan oligosaccharide, 0.03 ~
0.04% gibberellin, 0.01 ~ 0.03% methyl α-naphthyl acetate, 0.004 ~ 0.006% abscisic acid, 0.2 ~ 0.5% sucrose, 0.1 ~ 0.2% alginic acid
Sodium, balance of water；
（3）Cuttings cuttage：
By step（2）Cuttings oblique cutting after process keeps the eye of cuttings on the upside of branch on cuttage and seedling culture bed, inserts during cuttage
Fringe is 30 ~ 40 ° with the inclination angle of vertical direction；
（4）Manage after cuttage：
A. grow before 3 ~ 4 true leaves to cuttage seeding after cuttings cuttage, ultrasound-microwave alternate treatment is carried out to cuttings, it is described super
Sound-microwave alternate treatment is referred to and first cuttings ultrasonic radiation processed after 3 ~ 4h, and then with microwave to cuttings microwave with ultrasonic wave
The applying frequency of 2 ~ 3h of radiation treatment, wherein ultrasonic wave is 51 ~ 53kHz, and the applying power of microwave is 200 ~ 240W, every other day
Carry out once ultrasound-microwave alternate treatment；
B. grow during 3 ~ 4 true leaf to cuttage seedings grow up to a height of 28 ~ 32cm seedlings in cuttage seeding, every 13 ~ 15 days to cuttage
Seedling applies a ultraviolet light irradiation and processes, and the ultraviolet light irradiation is processed and referred to the ultraviolet light that wavelength is 270 ~ 300nm to skewer
Seedlings plugging radiation treatment, when a length of 6 ~ 8h per treatment；
（5）Into transplantation of seedlings：
When cuttage seeding grows up to the seedling of a height of 28 ~ 32cm, nursery is completed, then can be carried out after the hardening treatment of 3 ~ 5 days
Transplant operation.
Further, step（4）Described in ultrasound-microwave alternate treatment carry out every morning 7.00.
Further, step（4）Described in ultraviolet light irradiation process and carried out every morning 5.00.
The present invention has the advantages that：
Present invention improves the drawbacks of traditional cottage method, special process, the use before cuttage have been carried out during cuttage
Reason immersion bubble, enhances well the vigor of cuttings root, improves the overall activity of cuttings, it is ensured that root survival,
To growing before 3 ~ 4 true leaves after cuttage, the ultrasound for carrying out-microwave alternate treatment can change well to cuttage seeding overall permanence
Property improve and process, development and the vigor of its root system can be strengthened, improve its adaptability to adverse circumstances, the applying of ultrasound and microwave
With synergy, the ultraviolet light irradiation for carrying out at later stages processes the maintenance and lifting for being conducive to cuttage seeding overall permanence,
Accelerate its speed of growth, further improve its resistance.The inventive method can well improve the survival rate of grape cuttage, educate
Cuttage seeding cold resistance is strong, is adapted to industrial seedling rearing production.
Specific embodiment
Embodiment 1
A kind of strong grape cuttage seeding cultivating method of winter resistance, comprises the steps：
（1）Cuttings is chosen：
1 year raw branch for growing full sturdy, eye, no disease and pests harm, semi-lignified is chosen as skewer fringe, cuttings is intercepted a length of
15-18cm, retains 2 ~ 3 sections, and the otch of skewer fringe lower end will be near section portion or axil portion, and distance is 0.2-0.3cm, its otch
With sharp blade or razor, chamfer by 45° angle, imposed uniformity without examining individual cases disconnected to ensure that otch is smoothed, cuttings is obtained after cutting standby；
（2）Immersion treatment：
By step（1）In the cuttings bottom immersion treatment fluid of gained, it is 32 DEG C that heating keeps processing liquid temp, immersion treatment
Take out after 20min standby；The treatment fluid by following percentage by weight material composition：0.004% copper sulphate, 0.005% sulfuric acid
Zinc, 0.003% manganese sulfate, 0.008% ferrous sulfate, 0.01% boric acid, 0.004% sodium molybdate, 0.03% polyethylene glycol, 0.04% fruit
Sugar, 0.03% chitosan oligosaccharide, 0.03% gibberellin, 0.01% methyl α-naphthyl acetate, 0.004% abscisic acid, 0.2% sucrose, 0.1% sodium alginate, it is remaining
Measure as water；
（3）Cuttings cuttage：
By step（2）Cuttings oblique cutting after process keeps the eye of cuttings on the upside of branch on cuttage and seedling culture bed, inserts during cuttage
Fringe is 30 ~ 35 ° with the inclination angle of vertical direction；
（4）Manage after cuttage：
A. grow before 3 ~ 4 true leaves to cuttage seeding after cuttings cuttage, ultrasound-microwave alternate treatment is carried out to cuttings, it is described super
Sound-microwave alternate treatment is referred to and first cuttings ultrasonic radiation processed after 3h, and then with microwave to cuttings microwave spoke with ultrasonic wave
Process 2h is penetrated, the wherein applying frequency of ultrasonic wave is 51kHz, and the applying power of microwave is 200W, is every other day once surpassed
Sound-microwave alternate treatment；
B. grow during 3 ~ 4 true leaf to cuttage seedings grow up to a height of 28 ~ 30cm seedlings in cuttage seeding, cuttage seeding is applied every 13 days
Plus a ultraviolet light irradiation is processed, the ultraviolet light irradiation is processed and referred to the ultraviolet light that wavelength is 270 ~ 280nm to cuttage seeding
Radiation treatment, when a length of 6h per treatment；
（5）Into transplantation of seedlings：
When cuttage seeding grows up to the seedling of a height of 28 ~ 30cm, nursery is completed, then can be moved after the hardening treatment of 3 days
Plant operation.
Further, step（4）Described in ultrasound-microwave alternate treatment carry out every morning 7.00.
Further, step（4）Described in ultraviolet light irradiation process and carried out every morning 5.00.
Embodiment 2
A kind of strong grape cuttage seeding cultivating method of winter resistance, comprises the steps：
（1）Cuttings is chosen：
1 year raw branch for growing full sturdy, eye, no disease and pests harm, semi-lignified is chosen as skewer fringe, cuttings is intercepted a length of
16-18cm, retains 2 ~ 3 sections, and the otch of skewer fringe lower end will be near section portion or axil portion, and distance is 0.2-0.3cm, its otch
With sharp blade or razor, chamfer by 45° angle, imposed uniformity without examining individual cases disconnected to ensure that otch is smoothed, cuttings is obtained after cutting standby；
（2）Immersion treatment：
By step（1）In the cuttings bottom immersion treatment fluid of gained, it is 35 DEG C that heating keeps processing liquid temp, immersion treatment
Take out after 25min standby；The treatment fluid by following percentage by weight material composition：0.006% copper sulphate, 0.007% sulfuric acid
Zinc, 0.005% manganese sulfate, 0.01% ferrous sulfate, 0.013% boric acid, 0.006% sodium molybdate, 0.05% polyethylene glycol, 0.07% fruit
Sugar, 0.06% chitosan oligosaccharide, 0.04% gibberellin, 0.03% methyl α-naphthyl acetate, 0.006% abscisic acid, 0.5% sucrose, 0.2% sodium alginate, it is remaining
Measure as water；
（3）Cuttings cuttage：
By step（2）Cuttings oblique cutting after process keeps the eye of cuttings on the upside of branch on cuttage and seedling culture bed, inserts during cuttage
Fringe is 35 ~ 40 ° with the inclination angle of vertical direction；
（4）Manage after cuttage：
A. grow before 3 ~ 4 true leaves to cuttage seeding after cuttings cuttage, ultrasound-microwave alternate treatment is carried out to cuttings, it is described super
Sound-microwave alternate treatment is referred to and first cuttings ultrasonic radiation processed after 4h, and then with microwave to cuttings microwave spoke with ultrasonic wave
Process 3h is penetrated, the wherein applying frequency of ultrasonic wave is 53kHz, and the applying power of microwave is 240W, is every other day once surpassed
Sound-microwave alternate treatment；
B. grow during 3 ~ 4 true leaf to cuttage seedings grow up to a height of 30 ~ 32cm seedlings in cuttage seeding, cuttage seeding is applied every 15 days
Plus a ultraviolet light irradiation is processed, the ultraviolet light irradiation is processed and referred to the ultraviolet light that wavelength is 290 ~ 300nm to cuttage seeding
Radiation treatment, when a length of 8h per treatment；
（5）Into transplantation of seedlings：
When cuttage seeding grows up to the seedling of a height of 30 ~ 32cm, nursery is completed, then can be moved after the hardening treatment of 5 days
Plant operation.
Further, step（4）Described in ultrasound-microwave alternate treatment carry out every morning 7.00.
Further, step（4）Described in ultraviolet light irradiation process and carried out every morning 5.00.
Comparative example 1
This comparative example 1 compared with Example 1, in step（4）In the operation a managed after cuttage, ultrasonication is not carried out,
With waiting microwave treatment of duration to replace, method and step all same in addition.
Comparative example 2
This comparative example 2 compared with Example 1, in step（4）In the operation a managed after cuttage, do not carry out at microwave
Reason, with etc. the ultrasonication of duration replace, method and step all same in addition.
Comparative example 3
This comparative example 3 compared with Example 2, saves step（4）The operation b managed after cuttage, method and step in addition
All same.
Control group
Existing conventional grape cottage method.
In order to contrast effect of the present invention, the grape of " huge peak " kind is chosen as experimental subjects, with a collection of grape stalk,
Carry out cuttage and seedling culture with above-mentioned six kinds of methods respectively, educate after seedling, then be transplanted under low temperature environment, observe it and survive
Rate, table 1 below is corresponding correction data：
Table 1
| Cuttage survival rate（%） | Low temperature survival rate（%） | |
| Embodiment 1 | 99.0 | 90.6 |
| Embodiment 2 | 99.3 | 91.0 |
| Comparative example 1 | 95.1 | 76.2 |
| Comparative example 2 | 94.8 | 77.3 |
| Comparative example 3 | 96.3 | 81.6 |
| Control group | 90.5 | 69.4 |
Note：Cuttage survival rate described in upper table 1 refer to cuttings start to seedling from cuttage during survival rate；The low temperature into
Motility rate refers to and seedling is transplanted in the environment of -10 DEG C, carries out identical management, counts the survival rate in one month.
The survival rate of cuttage can be effectively improved by can be seen that the inventive method in upper table 1, strengthening the low temperature resistant of cuttage seeding
Performance, there is good promotional value.
The root system of seedling is bred as to embodiment 2 and control group carries out detection discovery, proline, solubility in the former root system
Sugar, soluble protein content lift more than 20% compared with the latter, are the main causes that its cold resistance is lifted.
Claims (3)
1. the strong grape cuttage seeding cultivating method of a kind of winter resistance, it is characterised in that comprise the steps：
（1）Cuttings is chosen：
1 year raw branch for growing full sturdy, eye, no disease and pests harm, semi-lignified is chosen as skewer fringe, cuttings is intercepted a length of
14-18cm, retains 2 ~ 3 sections, and the otch of skewer fringe lower end will be near section portion or axil portion, and distance is 0.2-0.3cm, its otch
With sharp blade or razor, chamfer by 45° angle, imposed uniformity without examining individual cases disconnected to ensure that otch is smoothed, cuttings is obtained after cutting standby；
（2）Immersion treatment：
By step（1）In the cuttings bottom immersion treatment fluid of gained, it is 32 ~ 35 DEG C that heating keeps processing liquid temp, immersion treatment
Take out after 20 ~ 25min standby；The treatment fluid by following percentage by weight material composition：0.004 ~ 0.006% copper sulphate,
0.005 ~ 0.007% zinc sulfate, 0.003 ~ 0.005% manganese sulfate, 0.008 ~ 0.01% ferrous sulfate, 0.01 ~ 0.013% boric acid,
0.004 ~ 0.006% sodium molybdate, 0.03 ~ 0.05% polyethylene glycol, 0.04 ~ 0.07% fructose, 0.03 ~ 0.06% chitosan oligosaccharide, 0.03 ~
0.04% gibberellin, 0.01 ~ 0.03% methyl α-naphthyl acetate, 0.004 ~ 0.006% abscisic acid, 0.2 ~ 0.5% sucrose, 0.1 ~ 0.2% alginic acid
Sodium, balance of water；
（3）Cuttings cuttage：
By step（2）Cuttings oblique cutting after process keeps the eye of cuttings on the upside of branch on cuttage and seedling culture bed, inserts during cuttage
Fringe is 30 ~ 40 ° with the inclination angle of vertical direction；
（4）Manage after cuttage：
A. grow before 3 ~ 4 true leaves to cuttage seeding after cuttings cuttage, ultrasound-microwave alternate treatment is carried out to cuttings, it is described super
Sound-microwave alternate treatment is referred to and first cuttings ultrasonic radiation processed after 3 ~ 4h, and then with microwave to cuttings microwave with ultrasonic wave
The applying frequency of 2 ~ 3h of radiation treatment, wherein ultrasonic wave is 51 ~ 53kHz, and the applying power of microwave is 200 ~ 240W, every other day
Carry out once ultrasound-microwave alternate treatment；
B. grow during 3 ~ 4 true leaf to cuttage seedings grow up to a height of 28 ~ 32cm seedlings in cuttage seeding, every 13 ~ 15 days to cuttage
Seedling applies a ultraviolet light irradiation and processes, and the ultraviolet light irradiation is processed and referred to the ultraviolet light that wavelength is 270 ~ 300nm to skewer
Seedlings plugging radiation treatment, when a length of 6 ~ 8h per treatment；
（5）Into transplantation of seedlings：
When cuttage seeding grows up to the seedling of a height of 28 ~ 32cm, nursery is completed, then can be carried out after the hardening treatment of 3 ~ 5 days
Transplant operation.
2. a kind of strong grape cuttage seeding cultivating method of winter resistance according to claim 1, it is characterised in that step（4）
Described in ultrasound-microwave alternate treatment carry out every morning 7.00.
3. a kind of strong grape cuttage seeding cultivating method of winter resistance according to claim 1, it is characterised in that step（4）
Described in ultraviolet light irradiation process and carried out every morning 5.00.
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| CN201611057006.8A CN106613718A (en) | 2016-11-26 | 2016-11-26 | Cultivation method for grape cutting seedlings with high cold resistance |
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| CN201611057006.8A CN106613718A (en) | 2016-11-26 | 2016-11-26 | Cultivation method for grape cutting seedlings with high cold resistance |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107041276A (en) * | 2017-06-22 | 2017-08-15 | 安庆市双益生态农业有限公司 | A kind of high-yield planting method of high-quality grapes |
| CN107125009A (en) * | 2017-06-22 | 2017-09-05 | 和县赭洛山茶叶种植专业合作社 | A kind of sweet osmanthus cottage breeding method |
| CN107950216A (en) * | 2017-12-21 | 2018-04-24 | 安庆市枞阳县阳和苗圃 | A kind of breeding method of grape cuttage seeding |
| CN108617430A (en) * | 2018-05-16 | 2018-10-09 | 内江师范学院 | A kind of mating system of cold-resistant ornamental value silk screen rattan seedling |
| CN110839651A (en) * | 2019-11-21 | 2020-02-28 | 广西壮族自治区农业科学院 | Vitis heyneana rooting agent and Vitis heyneana cutting seedling method |
| CN112567990A (en) * | 2020-11-28 | 2021-03-30 | 巢湖市年晟农业生态有限公司 | Cutting seedling method for improving survival rate of tea trees in alpine mountain areas |
| CN112567989A (en) * | 2020-11-28 | 2021-03-30 | 巢湖市年晟农业生态有限公司 | Ecological planting method for improving quality of tea in alpine mountain area |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107041276A (en) * | 2017-06-22 | 2017-08-15 | 安庆市双益生态农业有限公司 | A kind of high-yield planting method of high-quality grapes |
| CN107125009A (en) * | 2017-06-22 | 2017-09-05 | 和县赭洛山茶叶种植专业合作社 | A kind of sweet osmanthus cottage breeding method |
| CN107950216A (en) * | 2017-12-21 | 2018-04-24 | 安庆市枞阳县阳和苗圃 | A kind of breeding method of grape cuttage seeding |
| CN108617430A (en) * | 2018-05-16 | 2018-10-09 | 内江师范学院 | A kind of mating system of cold-resistant ornamental value silk screen rattan seedling |
| CN110839651A (en) * | 2019-11-21 | 2020-02-28 | 广西壮族自治区农业科学院 | Vitis heyneana rooting agent and Vitis heyneana cutting seedling method |
| CN112567990A (en) * | 2020-11-28 | 2021-03-30 | 巢湖市年晟农业生态有限公司 | Cutting seedling method for improving survival rate of tea trees in alpine mountain areas |
| CN112567989A (en) * | 2020-11-28 | 2021-03-30 | 巢湖市年晟农业生态有限公司 | Ecological planting method for improving quality of tea in alpine mountain area |
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Application publication date: 20170510 |