CN105165508A - High-yield sugar-increasing pear tree dwarf culture method - Google Patents
High-yield sugar-increasing pear tree dwarf culture method Download PDFInfo
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- CN105165508A CN105165508A CN201410251746.XA CN201410251746A CN105165508A CN 105165508 A CN105165508 A CN 105165508A CN 201410251746 A CN201410251746 A CN 201410251746A CN 105165508 A CN105165508 A CN 105165508A
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Abstract
The invention discloses a high-yield sugar-increasing pear tree dwarf culture method, characterized by comprising steps of (1) manufacturing a culture medium; (2) breeding seedlings; (3) planting; (4) transplanting; (5) and pruning and shaping. The culture medium properly increases saccharose and agar concentration, and browning phenomenon does not exist. A week after inoculation, a callus is generated from a bottom part. Growth vigour of the pear seedlings is in order, and rooting percentage is high. The method lays a foundation for improving yield later. The culture method improves disease resistance of seedlings, and provides abundant nutrient elements for growth of pear trees, so growth of pear tree is fast, and maneuverability is good, and yield of pear tree seeds is greatly increased. After pruning and shaping, dwarf effect of pear trees is realized, and obtained pear fruits are big and are high in sugar concentration. The method has obvious production increasing and sugar increasing effects.
Description
Technical field
The invention belongs to the technical field of cultivation of crop, be specifically related to a kind of pear tree dwarfing culture method that high yield increases sugar.
Background technology
Pear sapling is the same with other perennial woody fruit trees, its rooting of vitro seedling is by the impact of the many factors such as physiological age of its genotype, condition of culture, subculture number, plant, difficulty of taking root is large, some kind rooting rates are less than 30%, and condition of rooting is unstable, repeatable poor, constrain biotechnology breeding process and the choiceness Fast-propagation of pears to a great extent.In recent years, although more to the research of pears rooting of vitro seedling, all concentrate on medium, plant growth substance, activated carbon (AC) and osmotic pressure etc. to the impact of taking root, and few to the research report of the aspects such as root induction phase and root growth phase.For this reason, inquire into and affect the medium and condition of culture that pears take root, being intended to raising pears rooting rate, is the foundation instructing pears Fast-propagation and biotechnology breeding to provide certain.
At present, China's pear sapling cultivates general employing MS minimal medium, and compared with the basis of other medium, the content of the nitrate in MS medium, potassium and ammonium is high.The inorganic salt concentration that MS minimal medium is higher, very favourable to the growth of the mineral nutrition ensured needed for tissue growth and acceleration callus.Because the ion concentration in formula is high, in the processes such as preparation, storage, sterilization, even if some composition is slightly different, the also interionic balance of unlikely impact.MS solid culture medium can be used to evoked callus, or for embryo, stem section, stem apex and anther culture, can obtain obviously success when its liquid nutrient medium is used for cell suspension cultures.Quantity and the ratio of the inorganic nutrients in this medium are proper, be enough to meet plant cell nutritionally with physiological needs.
Current pocket culture requires the tree low body height of tree about 2 meters, is no more than 2.5 meters.Pocket culture is a kind of high-tech, normalized meticulous planting type: if tree body is too high, and artificial pollination, flower and fruit thinning, bagging, harvesting, spray medicine, to draw branch, pruning etc. to operate very inconvenient, not only time-consuming but also effect is bad.Windproof also harmful to combating a natural disaster.Stand in ground during best operated can raise one's hand and labor.Just can be regarded as stunted tree body.Most pears agriculture pruning aspect in the winter time, all adopts traditional trimming method: see that branch is just cut, (every root branch is cut all by the waist), sees that branch just stays (no matter intersect branch, overlapping branch all do not wipe out).Include some expert of certain technology, go to the countryside and instruct all in this way.And in the middle of actual management, major branch should be cut every year on the original basis under 50-60 centimetre again, impel raw 1-2 side shoot in next year, leading major branch still extends forward.Having the pears agriculture of managerial experiences all to know, can there is 2-3 young sprout at each clip, so prune every 50-60 centimeters, just have two side shoots in left and right next year in pear tree apical dominance strong (particularly emerald green hat, water-rich areas).And the pruning to side shoot, science is higher, if every root side shoot cuts off by the waist, 2-3 young sprout will be there is next year, occur side shoot raw side side shoot, and in scientific management angle, side shoot mainly cultivates short fruit bearing shoot, puts or light pruning so will grow, and could effectively achieve the goal.If side shoot cuts a cutter by the waist not only can not effective cultivation results brachyplast, and can bear unnecessary useless branch, upset tree-like, affect illumination, affect again the seed output and quality in the coming year.
Summary of the invention
The object of the present invention is to provide a kind of high yield to increase the pear tree dwarfing culture method of sugar, a kind of pear tree nanization high and stable yields cultivation method, to be conducive to the exploitation of pear tree.
The technical scheme that the present invention takes is:
High yield increases a pear tree dwarfing culture method for sugar, it is characterized in that, comprises the steps:
(1) make medium: get optimize MS basal liquid 15-18ml/L, sucrose 25-30g/L, agar 6-7g/L, indolebutyric acid IBA0.2-0.3mg/L, carragheen 7-9g/L mix, for subsequent use;
(2) seedling is cultivated: after getting Pear Bud sterilization treatment, as explant material, be inoculated in differential medium, within 20 days, change a subculture, change 3 subcultures and can differentiate pear tree seedling;
(3) plant: in booth, execute the base manure containing 0.6-0.8% dimethyl phosphoric acid pyrazoles, after ploughing, make the wide furrow for 4-5cm, by pear sapling with containing 0.5% carbendazim and the limewash of volumetric concentration 2% immersion 10-12min, with line-spacing 20-30cm, clump spacing 15-18cm is planted in furrow;
(4) transplant: treat that pear sapling sprouts the spray/bud that makes new advances, prune away when growing to 6-8cm pear tree seedling radical leaves, only stays stem apex end 3-5 blade, according to spacing in the rows 1.2m, line-spacing 2.1m transplants to field planting hole, waters sufficient normal root water;
(5) pruning integer: when Growth of Pear Trees is to 1-1.2m height, start to carry out pruning integer, pinching is bound, and cultivates most side shoot, every strain only retains a healthy and strong spray/bud, is trimmed to down umbrella shape tree-like.
The mixing fertilizer that base manure in described step (3) is urea, potassium chloride, diammonium phosphate are 5:3:2 composition according to weight ratio.
Beneficial effect of the present invention is:
1, medium of the present invention improves with pear tree face medium existing, suitably improves sucrose, agar concentration, and without browning, after inoculating one week, bottom produces callus, and pear sapling growing way is neat, and rooting rate is high, for follow-up raising output is laid a good foundation.
2, the seedling pretreatment before the plantation of this cultivation method improves the disease resistance of seedling, growth for pear tree provides abundant nutritive element, Growth of Pear Trees is fast, workable, the output of pear tree seed is able to significantly increase, the Dwarf Effect on Dwarf of pear tree is achieved after pruning integer, the pear fruit obtained is large and sugar content is high, has obvious volume increase, increases sugared effect.
Embodiment
Embodiment 1
High yield increases a pear tree dwarfing culture method for sugar, it is characterized in that, comprises the steps:
(1) make medium: get optimize MS basal liquid 15ml/L, sucrose 25g/L, agar 6g/L, indolebutyric acid IBA0.2mg/L, carragheen 7g/L mix, for subsequent use;
(2) seedling is cultivated: after getting Pear Bud sterilization treatment, as explant material, be inoculated in differential medium, within 20 days, change a subculture, change 3 subcultures and can differentiate pear tree seedling;
(3) plant: executing in booth is base manure that 5:3:2 form by urea, potassium chloride, diammonium phosphate according to weight ratio containing 0.6% dimethyl phosphoric acid pyrazoles, after ploughing, make the wide furrow for 4cm, by pear sapling with containing 0.5% carbendazim and the limewash of volumetric concentration 2% immersion 10min, with line-spacing 20cm, clump spacing 15cm is planted in furrow;
(4) transplant: treat that pear sapling sprouts the spray/bud that makes new advances, prune away when growing to 6cm pear tree seedling radical leaves, only stays stem apex end 3-5 blade, according to spacing in the rows 1.2m, line-spacing 2.1m transplants to field planting hole, waters sufficient normal root water;
(5) pruning integer: when Growth of Pear Trees is to 1m height, start to carry out pruning integer, pinching is bound, and cultivates most side shoot, every strain only retains a healthy and strong spray/bud, is trimmed to down umbrella shape tree-like.
Embodiment 2
High yield increases a pear tree dwarfing culture method for sugar, it is characterized in that, comprises the steps:
(1) make medium: get optimize MS basal liquid 18ml/L, sucrose 30g/L, agar 7g/L, indolebutyric acid IBA0.3mg/L, carragheen 9g/L mix, for subsequent use;
(2) seedling is cultivated: after getting Pear Bud sterilization treatment, as explant material, be inoculated in differential medium, within 20 days, change a subculture, change 3 subcultures and can differentiate pear tree seedling;
(3) plant: executing in booth is base manure that 5:3:2 form by urea, potassium chloride, diammonium phosphate according to weight ratio containing 0.8% dimethyl phosphoric acid pyrazoles, after ploughing, make the wide furrow for 5cm, by pear sapling with containing 0.5% carbendazim and the limewash of volumetric concentration 2% immersion 12min, with line-spacing 30cm, clump spacing 18cm is planted in furrow;
(4) transplant: treat that pear sapling sprouts the spray/bud that makes new advances, prune away when growing to 8cm pear tree seedling radical leaves, only stays stem apex end 3-5 blade, according to spacing in the rows 1.2m, line-spacing 2.1m transplants to field planting hole, waters sufficient normal root water;
(5) pruning integer: when Growth of Pear Trees is to 1.2m height, start to carry out pruning integer, pinching is bound, and cultivates most side shoot, every strain only retains a healthy and strong spray/bud, is trimmed to down umbrella shape tree-like.
Claims (2)
1. high yield increases a pear tree dwarfing culture method for sugar, it is characterized in that, comprises the steps:
(1) make medium: get optimize MS basal liquid 15-18ml/L, sucrose 25-30g/L, agar 6-7g/L, indolebutyric acid IBA0.2-0.3mg/L, carragheen 7-9g/L mix, for subsequent use;
(2) seedling is cultivated: after getting Pear Bud sterilization treatment, as explant material, be inoculated in differential medium, within 20 days, change a subculture, change 3 subcultures and can differentiate pear tree seedling;
(3) plant: in booth, execute the base manure containing 0.6-0.8% dimethyl phosphoric acid pyrazoles, after ploughing, make the wide furrow for 4-5cm, by pear sapling with containing 0.5% carbendazim and the limewash of volumetric concentration 2% immersion 10-12min, with line-spacing 20-30cm, clump spacing 15-18cm is planted in furrow;
(4) transplant: treat that pear sapling sprouts the spray/bud that makes new advances, prune away when growing to 6-8cm pear tree seedling radical leaves, only stays stem apex end 3-5 blade, according to spacing in the rows 1.2m, line-spacing 2.1m transplants to field planting hole, waters sufficient normal root water;
(5) pruning integer: when Growth of Pear Trees is to 1-1.2m height, start to carry out pruning integer, pinching is bound, and cultivates most side shoot, every strain only retains a healthy and strong spray/bud, is trimmed to down umbrella shape tree-like.
2. a kind of high yield according to claim 1 increases the pear tree dwarfing culture method of sugar, it is characterized in that, the base manure in described step (3) is urea, potassium chloride, diammonium phosphate be that the mixing of 5:3:2 composition is fertile according to weight ratio.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106900471A (en) * | 2017-04-25 | 2017-06-30 | 龙胜裕丰农业发展有限公司 | The cultural method of pear tree good quality and high output |
| CN107580957A (en) * | 2017-10-20 | 2018-01-16 | 太湖县香茗山生态果品科技园有限公司 | Pear tree high-yield planting method |
| CN107593220A (en) * | 2017-09-21 | 2018-01-19 | 山西省农业科学院果树研究所 | A kind of northern pear tree early ties the cultural method of high yield |
| CN111820062A (en) * | 2020-07-20 | 2020-10-27 | 湖北省农业科学院果树茶叶研究所 | Method for improving fruit setting rate, fruit quality and field yield of Hubei pear No. 2 and application thereof |
| CN113016483A (en) * | 2021-03-09 | 2021-06-25 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Dwarfing cultivation method for relieving top advantage of mango tree |
-
2014
- 2014-06-09 CN CN201410251746.XA patent/CN105165508A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106900471A (en) * | 2017-04-25 | 2017-06-30 | 龙胜裕丰农业发展有限公司 | The cultural method of pear tree good quality and high output |
| CN107593220A (en) * | 2017-09-21 | 2018-01-19 | 山西省农业科学院果树研究所 | A kind of northern pear tree early ties the cultural method of high yield |
| CN107580957A (en) * | 2017-10-20 | 2018-01-16 | 太湖县香茗山生态果品科技园有限公司 | Pear tree high-yield planting method |
| CN111820062A (en) * | 2020-07-20 | 2020-10-27 | 湖北省农业科学院果树茶叶研究所 | Method for improving fruit setting rate, fruit quality and field yield of Hubei pear No. 2 and application thereof |
| CN113016483A (en) * | 2021-03-09 | 2021-06-25 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Dwarfing cultivation method for relieving top advantage of mango tree |
| CN113016483B (en) * | 2021-03-09 | 2024-06-07 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Dwarf cultivation method for slowing down top dominance of mango trees |
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