CN105706840A - Method for improving cutting propagation survival rate of clerodendrum trichotomum - Google Patents

Abstract

The invention discloses a method for improving the survival rate of cuttage propagation in Changshan, Haizhou: cut the twigs of the current year from an adult mother tree as cuttings, wrap them with damp cloth, and keep them fresh; The length is controlled at 8~10cm, and 1~2 leaves are reserved on the upper part and 1/2~2/3 of the leaves are cut off; after cutting, the lower incision is dipped in a small amount of viscous liquid; The depth is 1/2 of the length of the cuttings, the first leaf is exposed above the substrate, and the twig cuttings are carried out, and the cuttings are watered immediately after the cuttings; after that, manual management is carried out, and weeding and weeding are carried out every 2 weeks and sprayed with 0.2% carbendazim solution. Disinfection; transplant in time after the root system of Changshan in Haizhou becomes lignified. The Haizhou Changshan twig cutting rooting method of the present invention has a cutting survival rate of nearly 97.6%, and high-quality Haizhou Changshan seedlings can be obtained. This Haizhou Changshan clonal seedling breeding system has low cost, simple technical requirements, and is easy to popularize and apply. Using the method can greatly improve the efficiency of its seedling cultivation.

Description

A method for improving the survival rate of cutting propagation in Changshan, Haizhou

technical field

The invention belongs to the technical field of plant propagation, and in particular relates to a method for improving the survival rate of cutting propagation by using Haizhou Changshan twigs as materials.

Background technique

Haizhou Changshan ( Clerodendrumtrichotomum. Thunb) is a deciduous shrub or small tree of the Verbenaceae Daqing genus, with a height of 1.5-8m. It is widely distributed in more than 20 provinces in North China, East China, Central South and Southwest my country. Haizhou Changshan has large inflorescences, tender green or pink buds, white or reddish petals, bright red calyx, dark blue fruit, persistent calyx, flowering and fruit setting for half a year from June to November, and is highly ornamental . It is a rare excellent garden flower and tree for viewing flowers and fruits in summer and autumn. Haizhou Changshan is resistant to salt and alkali, water and humidity, and harmful gases, and has strong tolerance and resistance to adverse environments. It is an ideal tree species for greening in coastal areas and saline-alkali lands. Haizhou Changshan can be used as medicine for the whole body. It has the effects of dispelling rheumatism, clearing heat and diuresis, calming the liver and lowering blood pressure. Its branches and leaves can be used as biological pesticides to kill spider mites, cotton aphids and underground pests; the oil content of the seeds is 34.1%, which is a biodiesel plant worthy of popularization and planting. Therefore, it has a variety of extremely high research and development application values, but most of Haizhou Changshan is still wildly distributed at present, and there are few garden applications, and Haizhou Changshan seedlings are hard to find in the market.

The cutting propagation method has the advantages of high reproduction coefficient, simple operation and low cost. Compared with sowing propagation, it has the advantages of maintaining the excellent characteristics of the female parent variety. At present, there are few reports on the cuttings of Haizhou Changshan twigs. In order to promote the large-scale seedling production of Haizhou Changshan, it is necessary to invent and create the cutting propagation technology of Haizhou Changshan.

Contents of the invention

Purpose of the present invention: aiming at the deficiencies in the prior art, the present invention provides a high-efficiency Haizhou Changshan cutting breeding technology, which is used to improve the efficiency of Haizhou Changshan seedling breeding, reduce the cost of Haizhou Changshan seedlings, and shorten the sea area. It is of great significance for the large-scale rapid propagation and cultivation promotion of Changshan in Haizhou to ensure the seedling cycle of Changshan improved varieties in Haizhou and ensure that the traits of excellent strains can be inherited stably.

Technical solution: In order to realize the above-mentioned purpose of the invention, the technical solution adopted in the present invention is as follows:

A method to improve the survival rate of cutting propagation in Changshan, Haizhou: cut the twigs of the current year from the adult mother tree as cuttings, wrap them with damp cloth, and keep them fresh; cut the cuttings flat, cut obliquely at the bottom, and control the length of the cuttings to 8 ~10cm, keep 1~2 leaves on the upper part and cut off 1/2~2/3 of the leaves; after cutting, dip a small amount of viscous liquid in the lower incision; on the substrate, the row spacing of plants is 8~10cm, and the depth of cuttings is the length of cuttings 1/2 of the first leaf was exposed above the substrate, and the twig cuttings were carried out, and the water was poured immediately after the cuttings; after that, manual management was carried out, and 0.2% carbendazim solution was sprayed for disinfection every 2 weeks; Transplant in time after the root system of Changshan in Changzhou becomes lignified. Among them, the viscous liquid is prepared by mixing 500~1000mg/L of naphthaleneacetic acid, 10~20g/L of hymexazol and 450~550g/L of talcum powder.

According to the method for improving the survival rate of cutting propagation in Changshan, Haizhou, the cuttings come from the semi-lignified green branches of the adult mother tree in the same year, and the middle and upper parts of the leafy branches that grow vigorously and are free of diseases and insect pests are selected. The upper incision on the cuttings is 1 cm away from the first bud point, and the lower incision is located at 0.5 cm from the bud to the bottom bud.

In the method for improving the survival rate of cutting propagation in Changshan, Haizhou, the substrate is peat and yellow mud with a volume ratio of 1:1.

According to the method for improving the survival rate of cutting propagation in Changshan, Haizhou, the time for cutting young shoots is from May to June.

According to the method for improving the survival rate of cutting propagation in Changshan, Haizhou, callus tissue is formed 10 to 15 days after cutting, adventitious roots appear in 20 to 25 days, and a large number of adventitious roots emerge after 30 days.

Beneficial effects: Compared with the prior art, the method for cutting and propagating the soft branches of Haizhou Changshan of the present invention adopts the viscous liquid prepared by mixing 400mg/L of naphthaleneacetic acid, 10-20g/L of hymexazol and talcum powder as the rooting method. Accelerator, which can prolong the action time of the accelerator, and at the same time play the role of sterilizing the base of the cuttings, avoiding the rot of the base of the cuttings, greatly improving the survival rate of the cuttings, forming calluses 10~15 days after cutting, and 20~ Adventitious roots appeared in 25 days, and a large number of adventitious roots emerged after 30 days. The survival rate of cuttings was nearly 97.6%. The Haizhou Changshan asexual seedling raising technology established by this method can obtain high-quality seedlings. The method is simple to operate, easy to master, reduces production cost, has good practicability, is easy to popularize and apply, and the like.

Description of drawings

Fig. 1 is a picture of the forming callus of Changshan twig cuttings in Haizhou;

Figure 2 shows the adventitious roots produced by cuttings of Changshan twigs in Haizhou.

detailed description

The present invention will be further described below in conjunction with specific examples. The following descriptions are only preferred embodiments of the present invention, and are only used to describe the present invention, and should not be construed as limiting the scope of the present invention. It should be noted that any modifications, equivalent replacements and improvements made within the spirit and principles of the present invention shall be included within the protection scope of the present invention.

Example 1

The test site is located in the Baima Teaching and Research Base of Nanjing Forestry University, located in Baima Town, Lishui District, Nanjing City, with four distinct seasons, mild and humid climate, abundant rainfall, sufficient sunlight, long frost-free period, and the same season of water and heat. The annual average temperature is 15.4°C, the average sunshine is 2240h, and the frost-free period is 237 days. The annual average rainfall is 1087mm, which is a typical low hilly area in southern Jiangsu.

The young shoots of the current year were collected from the adult Haizhou Changshan mother tree planted in the Baima base as test materials, and the middle and upper parts of the leafy branches that grew robustly and were free from diseases and insect pests were selected. The twig cuttings were collected in the afternoon of June 20, 2015, and the cutting time was in the morning of June 21. The experiment was carried out in the full-light intermittent cutting pond of Baima Base, and three different substrates were selected for the cutting of Haizhou Changshan twigs, namely, perlite, peat: yellow mud=1:1 (volume ratio), and yellow mud. Three repetitions were used in the experiment, and 100 twig cuttings were used in each repetition, and the viscous solution prepared without rooting promoter and fungicide was used as the control of each corresponding substrate.

Cut the twigs of the current year from the adult mother tree as cuttings, wrap them in a damp cloth to keep them fresh, and take them back indoors to cut the cuttings. Cut the upper incision of the cuttings flat, and cut the lower incision obliquely. The length of the cuttings is controlled at 8-10 cm, and 1-2 leaves are reserved on the upper part and 1/2-2/3 of the leaves are cut off.

After cutting, dip in the lower incision to take a small amount of viscous liquid prepared by mixing 500~1000mg/L of naphthaleneacetic acid, 10~20g/L of hymexazol and 450~550g/L of talcum powder. On the substrate, the distance between plants and rows is 8~10cm, and the depth of cuttings is about 1/2 of the length of cuttings. The first leaf is exposed above the substrate, and the young shoots are cut, and watered immediately after the cuttings; after that, manual management is carried out every 2 weeks. Cultivation and weeding and spraying 0.2% carbendazim solution for disinfection; transplanting in time after the root system of Changshan, Haizhou is lignified. Observe regularly during the cutting, and record the callus formation time (days), decay rate (%), survival rate (%) and root number.

After cutting, calluses formed at the base of the cuttings in 10-15 days, as shown in Figure 1, and adventitious roots appeared in 20-25 days, and as shown in Figure 2, a large number of adventitious roots emerged after 30 days.

The results of Haizhou Changshan twig cuttings in the three substrates are shown in Table 1. The survival rate is the most important indicator to measure the success of cutting seedlings. The decay rate of Haizhou Changshan cuttings with perlite as the matrix is 12.3%, the rooting rate is 78.3%, and the average root number is 5.3; the decay rate of Haizhou Changshan cuttings in the yellow mud matrix is 11.5%, and the rooting rate is 82.4% , with an average root number of 6.1; while using peat: yellow mud as the substrate for cuttings, the rot rate of the cuttings was only 1.7%, the rooting rate was 97.6%, and the average root number was 9.8. Compared with the corresponding control, the survival rate has been greatly improved (see Table 1). It can be seen that, adopting this method to carry out Haizhou Changshan twig cuttings, supplemented with a small amount of naphthalene acetic acid 400mg/L, hymexazol 10~20g/L and talcum powder mixed with viscous liquid as a multifunctional rooting accelerator. Cutting on the substrate of peat: yellow mud can significantly improve the survival rate of cuttings in Changshan, Haizhou.

Table 1 Test data statistics

Claims (6)

1. the method improving clerodendron trichotomum cottage propagation survival rate, it is characterised in that: on the adult elite stand of clip, raw twig, as cutting, is wrapped with wet cloth then, keeps fresh state；Being cut flat by cutting upper cut, lower cut is tiltedly cut, and cutting length controls at 8 ~ 10cm, and top retains 1 ~ 2 leaf and blade is cut off 1/2 ~ 2/3；Shear rear lower cut and dip a small amount of viscous solution；With seeding row spacing 8 ~ 10cm in substrate, the cutting degree of depth is the 1/2 of cutting length, and first blade exposes on substrate, carries out epicormic branch cuttage, cuttage is complete water immediately permeable；Carry out afterwards labor management and within every 2 weeks, carry out middle weeding and spray 0.2% carbendazim solution carry out disinfection；Transplant in time after clerodendron trichotomum root system lignifying；Wherein, viscous solution is hybridly prepared into by naphthalene acetic acid 500 ~ 1000mg/L, hymexazol 10 ~ 20g/L and Pulvis Talci 450 ~ 550g/L.

2. the method for raising clerodendron trichotomum cottage propagation survival rate according to claim 1, it is characterised in that: cutting comes from adult elite stand raw semi-lignified green branch then, chooses robust growth, the middle and upper part with blade branch without pest and disease damage.

3. the method for raising clerodendron trichotomum cottage propagation survival rate according to claim 1, it is characterised in that: first bud point 1cm of cutting upper cut distance, lower cut is positioned at bud from bottom bud 0.5cm.

4. the method for raising clerodendron trichotomum cottage propagation survival rate according to claim 1, it is characterised in that: substrate is peat and the yellow mud of volume ratio 1:1.

5. the method for raising clerodendron trichotomum cottage propagation survival rate according to claim 1, it is characterised in that: the time of epicormic branch cuttage is 5 ~ June.

6. the method for raising clerodendron trichotomum cottage propagation survival rate according to claim 1, it is characterised in that: within after cuttage 10 ~ 15 days, form callus, adventitious root occurred in 20 ~ 25 days, after 30 days, emerge in large numbers a large amount of adventitious root.