CN109329051A - Jasmine quickly breeds the preparation method with culture medium - Google Patents
Jasmine quickly breeds the preparation method with culture medium Download PDFInfo
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- CN109329051A CN109329051A CN201811168493.4A CN201811168493A CN109329051A CN 109329051 A CN109329051 A CN 109329051A CN 201811168493 A CN201811168493 A CN 201811168493A CN 109329051 A CN109329051 A CN 109329051A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/30—Growth substrates; Culture media; Apparatus or methods therefor based on or containing synthetic organic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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Abstract
The present invention provides Jasmine and quickly breeds the preparation method for using culture medium, belong to jasmine breeding field, comprising: 1 preparation initial culture base sequentially adds KT, CTK of formula ratio, sucrose, lucid asparagus extracting solution, agar into MS culture medium to being completely dissolved, adjust pH, high pressure sterilization；2 prepare proliferated culture medium, and the KT of formula ratio, acetic acid, sucrose, agar are sequentially added into MS culture medium to being completely dissolved, adjusts pH, high pressure sterilization；Heteroauxin is added after cooling；3 prepare root media: sequentially adding KT, sucrose, coconut juice, the tender willow juice, agar of formula ratio into MS culture medium, adjust pH, high pressure sterilization.Have the beneficial effect that this preparation method is simple and easy to do, cheap environmental protection, clearly different culture medium is provided for the different phase that Jasmine quickly breeds, Jasmine different reproductive period can be met to the different demands of different-energy substance and the substance that comes into force, promote seedling to grow up healthy and sound, increase robust seedling seedling.
Description
Technical field
The invention belongs to Jasmine breeding fields, and in particular to Jasmine quickly breeds the preparation method with culture medium.
Background technique
Jasmine [Jasminum sambac (L.) Aiton] is Oleaceae jasminum evergreen shrubs, tree crown in vertical or
Half carries out shape, originates in tropical and subtropical region.Common jasmine kind has the jasmine that overgrows, woody jasmine, mound jasmine, ocean
More than 60 kinds of jasmine etc., single-lobe jasmine, bivalve jasmine, 3 kinds of more valve jasmines can be divided into according to its flower-shape construction.China is jasmine in the world
The most country of jasmine yield, annual output account for 60% of Gross World Product or more, now with the ground such as Guangxi, Fujian, Guangdong, Yunnan kind
Plant is the most extensive, and wherein Guangxi is the maximum place of production of China Jasmine, accounts for the 80% of national yield.
However, Jasmine cultivar is single, Hengxian County locality bivalve jasmine, and pest and disease damage are only cultivated for a long time
Gradually increase, as southern blight has begun to extend；Jasmine Natural seed setting rate is low, if bivalve jasmine is only 0.19%；After sexual propagation
Generation separation is serious, mostly uses cutting propagation in production, but long-term vegetative propagation cause jasmine kind sexual involution, resistance decline,
Flower yield reduces year by year.Therefore, the commercialization breeding that should carry out jasmine breeding using group culturation rapid propagating technology as early as possible, passes through mother
The rejuvenation and screening for setting explant remake in vitro culture, can further improve seedling quality；In addition it is also possible to be sieved by bud mutation
Choosing obtains new lines.Explant material needed for tissue culture is seldom, and growth coefficient is high, and cuttage then needs a large amount of branches.Therefore,
The breeding of breeding should be based on tissue culture approach, and during tissue culture, and culture medium is to the condition of rooting of jasmine and survives situation to closing
It is important.
Summary of the invention
The purpose of the present invention is to provide the preparation method of the quick breeding culture medium of Jasmine, the preparation sides of culture medium
Method is simple, and the biological function of the active material of rush differentiation, growth-promoting bud, growth-promoting root in culture medium can arrive preferable preservation,
The nutritional need that the quick breeding culture medium from jasmine flower seed to rooted seedling all the period of time can be met, is remarkably improved and takes root
The robustness of rate and root.
For achieving the above object, the present invention the following technical schemes are provided:
The present invention provides a kind of Jasmine quickly breeding culture mediums, including initial culture base, proliferated culture medium and life
Root culture medium；
The initial culture base are as follows: containing 0.12~0.15mg/L KT, 5~10mg/L CTK, 22~25g/L sucrose,
The MS culture medium of 12~15g/L lucid asparagus extracting solution, 8~10g/L agar, pH are 6.4~6.6；Initial culture base can meet jasmine
Jasmine organizes the nutritional need tentatively cultivated, and CTK can promote cell elongation and division, is ready for further Multiplying culture,
Lucid asparagus extracting solution can effectively avoid jasmine tissue and browning, corruption occur, and promotes its histocyte smoothly to divide, break up, improves
The vigor and robustness of acquired tissue-cultured seedling are conducive to subsequent Multiplying culture and culture of rootage；
The proliferated culture medium are as follows: contain 0.25~0.3mg/L KT, 5~6mg/L heteroauxin, 1~1.2mg/L
(-) -8- phenylmenthol, 1.5~2g/L acetic acid, 25~28g/L sucrose, 3~5g/L agar MS culture medium, pH be 6.5~
6.6；The generation that heteroauxin can promote cell differentiation, promote bud, but it is exposed under light and is easier to by light degradation be indoles and second
The second of (-) -8- phenylmenthol is added to lose the effect of its effect promotees differentiation, growth-promoting bud in acid in proliferated culture medium
Alcoholic solution can be effectively prevented the light degradation of heteroauxin, a small amount of (-) -8- phenylmenthol condition existing for ethyl alcohol
Under recombination reaction promptly can occur with heteroauxin, (-) -8- phenylmenthol-heteroauxin compound of generation can make
For heteroauxin a kind of stable preservation form and enter in plant, so as to avoid the light degradation of heteroauxin, appropriate
Under the conditions of, intracorporal (-) -8- phenylmenthol-heteroauxin compound that is somebody's turn to do of plant can dissociate heteroauxin and play life
Object effect induces the generation of axillary bud and adventitious bud, the quantity and quality of young shoot greatly in promotion Multiple Buds；
The root media are as follows: contain 0.3~0.4mg/L KT, 30~32g/L sucrose, 15~25g/L coconut juice, 1.5
The MS culture medium of the tender willow juice of~3g/L Semen Coicis leachate, 8~10g/L, 4~10g/L agar, pH are 6.5~6.8；Culture of rootage
Base can provide sufficient carbohydrate energy sources for the seedling by Multiplying culture, and Semen Coicis leachate can effectively be strengthened, regulate and control
The activity of the content of endogenous hormones and important enzyme in ase in Jasmine seedlings body, promotes the synthesis of large biological molecule, at the same induce main root and
The growth of lateral root adjusts metabolism degree, enhances the sturdy degree of seedlings root, reaches growth-promoting root, protects the effect of surviving, increases robust seedling seedling,
Nursery reproductive efficiency is greatly improved, economic benefit is expanded.
The melting brown rate and Decayed rate of Jasmine tissue block can be significantly reduced using culture medium prescription provided by the invention, together
Its rooting rate of Shi Tigao and survival rate, root system is huge, sturdy, improves reproductive efficiency, shortens the breeding cycle, increases economic efficiency,
Significantly more efficient approach is provided for the industrialization production of Jasmine.
Preferably, initial culture base are as follows: contain 0.14mg/L KT, 8mg/L CTK, 24g/L sucrose, 15g/L lucid asparagus
The MS culture medium of extracting solution, 8g/L agar, pH 6.55；
Proliferated culture medium are as follows: contain 0.28mg/L KT, 5.5mg/L heteroauxin, 1.1mg/L (-) -8- phenyl peppermint
Alcohol, 1.8g/L acetic acid, 26g/L sucrose, 4g/L agar MS culture medium, pH 6.6；
Root media are as follows: contain 0.35mg/L KT, 30g/L sucrose, 20g/L coconut juice, 2g/L Semen Coicis leachate, 10g/
The MS culture medium of the tender willow juice of L, 8g/L agar, pH 6.7.
In the present invention using lucid asparagus extracting solution, Semen Coicis leachate, tender willow juice be all made of filtration sterilization by the way of carry out
Degerming had not only maintained the bioactivity of natural additive, but also being capable of effective degerming.
The present invention also provides a kind of preparation methods of the quick breeding culture medium of Jasmine, include the following steps:
Preparation initial culture base: 0.12~0.15mg/L KT, 5~10mg/L CTK, 22 are sequentially added into MS culture medium
To being completely dissolved, adjusting pH is 6.4~6.6 for~25g/L sucrose, 12~15g/L lucid asparagus extracting solution, 8~10g/L agar, in
121 DEG C of 15~25min of high pressure sterilization；CTK can promote cell elongation and division in initial culture base, for further proliferation training
It supports and is ready, lucid asparagus extracting solution can effectively avoid jasmine tissue and browning, corruption occurs, its histocyte is promoted smoothly to divide
It splits, break up, improve the vigor and robustness of acquired tissue-cultured seedling, be conducive to subsequent Multiplying culture and culture of rootage；
It prepares proliferated culture medium: sequentially adding 0.25~0.3mg/L KT, 1~1.2mg/L (-) -8- into MS culture medium
Phenylmenthol, 1.5~2g/L acetic acid, 25~28g/L sucrose, 3~5g/L agar to being completely dissolved, adjust pH be 6.4~
6.6, in 121 DEG C of 15~25min of high pressure sterilization, after cooling, 5~6mg/L heteroauxin is added at a temperature of half-light, 1~2 DEG C,
Half-light after dissolution saves at a temperature of 1~2 DEG C；The generation that heteroauxin can promote cell differentiation, promote bud, but it is exposed to light
Under be easier to by light degradation be indoles and acetic acid, so that the effect of its effect promotees differentiation, growth-promoting bud is lost, therefore in half-light, 1~2
Configuration facilitates storage life biological effectiveness at a temperature of DEG C, while (-) -8- phenylmenthol being added in proliferated culture medium
Ethanol solution can be effectively prevented the light degradation of heteroauxin, a small amount of (-) -8- phenylmenthol item existing for ethyl alcohol
Recombination reaction, (-) -8- phenylmenthol-heteroauxin compound of generation promptly can occur with heteroauxin under part
It can be used as a kind of stable preservation form of heteroauxin and enter in plant, so as to avoid the light degradation of heteroauxin,
Under felicity condition, intracorporal (-) -8- phenylmenthol-heteroauxin compound that is somebody's turn to do of plant can dissociate heteroauxin and send out
Biological effectiveness is waved, the generation of axillary bud and adventitious bud is induced, the quantity and quality of young shoot greatly in promotion Multiple Buds；
It prepares root media: sequentially adding 0.3~0.4mg/L KT, 30~32g/L sucrose, 15 into MS culture medium
~25g/L coconut juice, 1.5~3g/L Semen Coicis leachate, the tender willow juice of 8~10g/L, 4~10g/L agar, adjust pH be 6.5~
6.8, in 121 DEG C of 15~25min of high pressure sterilization；Root media can provide sufficient carbon water for the seedling by Multiplying culture
The work of the content of endogenous hormones and important enzyme in ase in Jasmine seedlings body can effectively be strengthened, be regulated and controled to the compound energy, Semen Coicis leachate
Property, promote the synthesis of large biological molecule, while inducing the growth of taproot and lateral root, adjust metabolism degree, enhancing seedlings root is thick
Strong degree reaches growth-promoting root, protects the effect of surviving, increase robust seedling seedling, greatly improve nursery reproductive efficiency, expands economic benefit.
Jasmine provided in the present invention quickly breeds with the preparation method of culture medium is simple and easy to do, cheap environmental protection, clear
Ground provides different culture medium for the different phase that Jasmine quickly breeds, and can meet Jasmine different reproductive period to difference
The different demands of energy matter and the substance that comes into force, had not only avoided the light degradation of auxin substance, but also can reduce it in jasmine
The intracorporal accumulation of seedling is encroached on caused by seedling, is promoted seedling to grow up healthy and sound, is increased robust seedling seedling.
Preferably, the acquisition methods of the lucid asparagus extracting solution are as follows: blend lucid asparagus spray tender leaf, according to solid-to-liquid ratio
Distilled water is added in 1:2.5~4, is stirred continuously 2~3 hours in 45~48 DEG C of temperature, filtering to take filtrate is lucid asparagus extraction
Liquid；The effective component that stirring is conducive in lucid asparagus spray tender leaf at high temperature is released, and lucid asparagus extracting solution can effectively avoid jasmine
Browning, corruption occur for jasmine tissue, its histocyte is promoted smoothly to divide, break up, and improve the vigor of acquired tissue-cultured seedling and are good for
Strong degree, is conducive to subsequent Multiplying culture and culture of rootage.
Preferably, the acquisition methods of the Semen Coicis leachate are as follows: as 2~3 times of weight after taking SEMEN COICIS to eluriate completely
In water, vinegar and white sugar are added after maceration, stirring dissolves white sugar completely, and room temperature extracts 36~48 hours, filters to take filtrate i.e.
Obtain Semen Coicis leachate；Addition vinegar and white sugar facilitate the leaching of effective component in SEMEN COICIS, the obtained heart of a lotus seed after Semen Coicis softening
The activity of the content of endogenous hormones and important enzyme in ase in Jasmine seedlings body can effectively be strengthened, be regulated and controled to rice leachate, promote biology big
The synthesis of molecule, while the growth of taproot and lateral root is induced, metabolism degree is adjusted, enhances the sturdy degree of seedlings root, reaches growth-promoting
The effect of root, guarantor survive, increases robust seedling seedling, greatly improves nursery reproductive efficiency, expands economic benefit.
As to present invention further optimization, in the acquisition methods of Semen Coicis leachate, the additive amount of vinegar is water weight
0.5~1%, the additive amount of white sugar is the 1% of water weight；Vinegar and white sugar facilitate the leaching of effective component in SEMEN COICIS,
The activity of the content of endogenous hormones and important enzyme in ase in Jasmine seedlings body can effectively be strengthened, be regulated and controled to obtained Semen Coicis leachate,
Promote the synthesis of large biological molecule, while inducing the growth of taproot and lateral root, adjust metabolism degree, enhancing seedlings root is sturdy
Degree reaches growth-promoting root, protects the effect of surviving.
Preferably, the acquisition methods of the tender willow juice are as follows: current year raw tender withy is taken, the soft rear dissection of pressing is concentrated, as
It is extracted in water, water had not had tender withy section just, persistently extracted 24~48 hours, took out withy section, removed insoluble matter up to tender willow
Juice.
The present invention at least has one of following advantage:
1) lucid asparagus extracting solution is added in initial culture base can effectively avoid jasmine tissue generation browning, corruption, promote it
Histocyte smoothly divides, breaks up, and improves the vigor and robustness of acquired tissue-cultured seedling, is conducive to subsequent Multiplying culture and life
Root culture；
2) (-) -8- phenylmenthol added in proliferated culture medium existing for the ethyl alcohol under the conditions of can promptly with Yin
Recombination reaction occurs for indolylbutyric acid, and the compound of generation can be used as a kind of stable preservation form of heteroauxin, avoid indoles second
The light degradation of acid, under felicity condition, plant it is intracorporal should (-) -8- phenylmenthol-heteroauxin compound can dissociate
Heteroauxin and play biological effectiveness, induce axillary bud and adventitious bud generation；
3) Semen Coicis leachate is added in root media, Semen Coicis leachate can effectively be strengthened, regulate and control ase in Jasmine seedlings body
The content of interior endogenous hormones and the activity of important enzyme, promote the synthesis of large biological molecule, while inducing the life of taproot and lateral root
It is long, metabolism degree is adjusted, the sturdy degree of seedlings root is enhanced, reaches growth-promoting root, protect the effect of surviving, increase robust seedling seedling, greatly improve
Nursery reproductive efficiency；
4) culture medium prescription provided by the invention can significantly reduce the melting brown rate and Decayed rate of Jasmine tissue block, simultaneously
Its rooting rate and survival rate are improved, root system is huge, sturdy, improves reproductive efficiency, shortens the breeding cycle, increases economic efficiency, be
The industrialization production of Jasmine provides significantly more efficient approach；
5) vinegar and white sugar are added after Semen Coicis softening facilitates the leaching of effective component in SEMEN COICIS, obtained Semen Coicis
The activity of the content of endogenous hormones and important enzyme in ase in Jasmine seedlings body can effectively be strengthened, be regulated and controled to leachate, promote big point of biology
The synthesis of son, while inducing the growth of taproot and lateral root；
6) this preparation method is simple and easy to do, cheap environmental protection, clearly provides for the different phase that Jasmine quickly breeds
Different culture medium can meet Jasmine different reproductive period to the different demands of different-energy substance and the substance that comes into force, both avoid
The light degradation of auxin substance, and it can be reduced in ase in Jasmine seedlings intracorporal accumulation infringement caused by seedling, promote
Seedling grows up healthy and sound, and increases robust seedling seedling.
Present invention employs above-mentioned technical proposals to provide the preparation method of the quick breeding culture medium of Jasmine, compensates for
The deficiencies in the prior art, reasonable design, easy operation.
Specific embodiment
The invention discloses a kind of preparation method of the quick breeding culture medium of Jasmine, those skilled in the art can be with
Present disclosure is used for reference, realization of process parameters is suitably modified.In particular, it should be pointed out that all similar substitutions and modifications are to this
It is it will be apparent that they are considered as being included in the present invention for the technical staff of field.Method and application of the invention is
Be described by preferred embodiment, related personnel obviously can not depart from the content of present invention, in spirit and scope to herein
The methods and applications are modified or appropriate changes and combinations, carry out implementation and application the technology of the present invention.
Present invention is further described in detail with reference to embodiments:
Embodiment 1:
A kind of Jasmine quickly breeds the preparation method with culture medium, includes the following steps:
Preparation initial culture base: sequentially added into MS culture medium 0.12mg/L KT, 5mg/L CTK, 22g/L sucrose,
12g/L lucid asparagus extracting solution, 8g/L agar are to being completely dissolved, and adjusting pH is 6.4, in 121 DEG C of high pressure sterilization 15min；Just it is commissioned to train
Supporting CTK in base can promote cell elongation and division, be ready for further Multiplying culture, lucid asparagus extracting solution can be effective
It avoids jasmine tissue that browning, corruption occurs, its histocyte is promoted smoothly to divide, break up, improve the work of acquired tissue-cultured seedling
Power and robustness are conducive to subsequent Multiplying culture and culture of rootage；
Prepare proliferated culture medium: sequentially added into MS culture medium 0.25mg/L KT, 1.5g/L acetic acid, 25g/L sucrose,
For 3g/L agar to being completely dissolved, adjusting pH is 6.4, in 121 DEG C of high pressure sterilization 15min, after cooling, at a temperature of half-light, 1 DEG C
5mg/L heteroauxin is added, half-light after dissolution saves at a temperature of 1 DEG C；The life that heteroauxin can promote cell differentiation, promote bud
At, the generation of induction axillary bud and adventitious bud, the quantity and quality of young shoot greatly in promotion Multiple Buds；
Prepare root media: sequentially added into MS culture medium 0.3mg/L KT, 30g/L sucrose, 15g/L coconut juice,
The tender willow juice of 1.5g/L Semen Coicis leachate, 8g/L, 4g/L agar, adjusting pH is 6.5, in 121 DEG C of high pressure sterilization 15min；It takes root training
Feeding base can provide sufficient carbohydrate energy sources for the seedling by Multiplying culture, and Semen Coicis leachate can effectively be strengthened, adjust
The content of endogenous hormones and the activity of important enzyme in ase in Jasmine seedlings body are controlled, promotes the synthesis of large biological molecule, while inducing main root
With the growth of lateral root, metabolism degree is adjusted, enhances the sturdy degree of seedlings root, reaches growth-promoting root, protect the effect of surviving, increase robust seedling
Seedling greatly improves nursery reproductive efficiency, expands economic benefit.
The acquisition methods of the lucid asparagus extracting solution are as follows: lucid asparagus spray tender leaf is blended, is added according to solid-to-liquid ratio 1:2.5
Enter distilled water, is stirred continuously 2 hours in 45 DEG C of temperature, filtering to take filtrate is lucid asparagus extracting solution；Stirring is advantageous at high temperature
Effective component in lucid asparagus spray tender leaf is released, and lucid asparagus extracting solution can effectively avoid jasmine tissue and browning, corruption occurs
It loses, its histocyte is promoted smoothly to divide, break up, improve the vigor and robustness of acquired tissue-cultured seedling, be conducive to subsequent proliferation
Culture and culture of rootage.
The acquisition methods of the Semen Coicis leachate are as follows: take SEMEN COICIS eluriate it is clean after as in the water of 2 times of weight, maceration
The vinegar of water weight 0.5% and 1% white sugar are added afterwards, stirring dissolves white sugar completely, and room temperature extracts 36 hours, filters to take
Filtrate is up to Semen Coicis leachate；Addition vinegar and white sugar facilitate the leaching of effective component in SEMEN COICIS, gained after Semen Coicis softening
To Semen Coicis leachate can effectively strengthen, regulate and control the activity of the content of endogenous hormones and important enzyme in ase in Jasmine seedlings body, promote
The synthesis of large biological molecule, while the growth of taproot and lateral root is induced, metabolism degree is adjusted, enhances the sturdy degree of seedlings root, reaches
To growth-promoting root, the effect of surviving is protected, increases robust seedling seedling, greatly improves nursery reproductive efficiency, expands economic benefit.
The acquisition methods of the tender willow juice are as follows: take current year raw tender withy, dissection after concentrating pressing soft, as soaking in water
It mentions, water had not had tender withy section just, persistently extracted 24 hours, took out withy section, removed insoluble matter up to tender willow juice.
Embodiment 2:
A kind of Jasmine quickly breeds the preparation method with culture medium, includes the following steps:
Preparation initial culture base: sequentially added into MS culture medium 0.15mg/L KT, 10mg/L CTK, 25g/L sucrose,
15g/L lucid asparagus extracting solution, 10g/L agar are to being completely dissolved, and adjusting pH is 6.6, in 121 DEG C of high pressure sterilization 25min；It is primary
CTK can promote cell elongation and division in culture medium, be ready for further Multiplying culture, lucid asparagus extracting solution can have
Effect avoids jasmine tissue that browning, corruption occurs, its histocyte is promoted smoothly to divide, break up, and improves acquired tissue-cultured seedling
Vigor and robustness are conducive to subsequent Multiplying culture and culture of rootage；
It prepares proliferated culture medium: sequentially adding 0.3mg/L KT, 1.2mg/L (-) -8- phenyl peppermint into MS culture medium
To being completely dissolved, adjusting pH is 6.6 for alcohol, 2g/L acetic acid, 28g/L sucrose, 5g/L agar, cold in 121 DEG C of high pressure sterilization 25min
But after, 6mg/L heteroauxin is added at a temperature of half-light, 2 DEG C, half-light after dissolution saves at a temperature of 2 DEG C；Heteroauxin can promote
Into cell differentiation, the generation of promotion bud, but it is exposed under light and is easier to by light degradation be indoles and acetic acid, to lose its function
Effect promotees the effect of differentiation, growth-promoting bud, therefore configuration facilitates storage life biological effectiveness under half-light, low temperature, while increasing
The ethanol solution for growing addition (-) -8- phenylmenthol in culture medium can be effectively prevented the light degradation of heteroauxin, on a small quantity
(-) -8- phenylmenthol existing for the ethyl alcohol under the conditions of recombination reaction promptly can occur with heteroauxin, generation
(-) -8- phenylmenthol-heteroauxin compound can be used as a kind of stable preservation form of heteroauxin and enter plant
Interior, so as to avoid the light degradation of heteroauxin, under proper condition, plant is intracorporal should (-) -8- phenylmenthol-indoles
Acetic acid compound can dissociate heteroauxin and play biological effectiveness, induce the generation of axillary bud and adventitious bud, greatly mention
Rise the quantity and quality of young shoot in Multiple Buds；
Prepare root media: sequentially added into MS culture medium 0.4mg/L KT, 32g/L sucrose, 25g/L coconut juice,
The tender willow juice of 3g/L Semen Coicis leachate, 10g/L, 10g/L agar, adjusting pH is 6.8, in 121 DEG C of high pressure sterilization 25min；It takes root training
Feeding base can provide sufficient carbohydrate energy sources for the seedling by Multiplying culture, and Semen Coicis leachate can effectively be strengthened, adjust
The content of endogenous hormones and the activity of important enzyme in ase in Jasmine seedlings body are controlled, promotes the synthesis of large biological molecule, while inducing main root
With the growth of lateral root, metabolism degree is adjusted, enhances the sturdy degree of seedlings root, reaches growth-promoting root, protect the effect of surviving, increase robust seedling
Seedling greatly improves nursery reproductive efficiency, expands economic benefit.
The acquisition methods of the lucid asparagus extracting solution are as follows: lucid asparagus spray tender leaf is blended, is added according to solid-to-liquid ratio 1:4
Distilled water is stirred continuously 3 hours in 48 DEG C of temperature, and filtering to take filtrate is lucid asparagus extracting solution.
The acquisition methods of the Semen Coicis leachate are as follows: take SEMEN COICIS eluriate it is clean after as in the water of 3 times of weight, bubble chamber
Temperature extraction 48 hours, filters to take filtrate up to Semen Coicis leachate.
The acquisition methods of the tender willow juice are as follows: take current year raw tender withy, dissection after concentrating pressing soft, as soaking in water
It mentions, water had not had tender withy section just, persistently extracted 48 hours, took out withy section, removed insoluble matter up to tender willow juice.
Embodiment 3:
A kind of Jasmine quickly breeds the preparation method with culture medium, includes the following steps:
Preparation initial culture base: sequentially added into MS culture medium 0.14mg/L KT, 8mg/L CTK, 24g/L sucrose,
8g/L agar is to being completely dissolved, and adjusting pH is 6.5, in 121 DEG C of high pressure sterilization 20min；CTK can promote carefully in initial culture base
Born of the same parents extend and division, are ready for further Multiplying culture, and it is brown that lucid asparagus extracting solution can effectively avoid jasmine tissue generation
Change, corruption, its histocyte is promoted smoothly to divide, break up, the vigor and robustness of acquired tissue-cultured seedling is improved, after being conducive to
Continuous Multiplying culture and culture of rootage；
It prepares proliferated culture medium: sequentially adding 0.28mg/L KT, 1mg/L (-) -8- phenyl peppermint into MS culture medium
To being completely dissolved, adjusting pH is 6.5 for alcohol, 1.8g/L acetic acid, 26g/L sucrose, 4g/L agar, in 121 DEG C of high pressure sterilization 20min,
After cooling, 5.5mg/L heteroauxin is added at a temperature of half-light, 1 DEG C, half-light after dissolution saves at a temperature of 1 DEG C；Heteroauxin
It can promote cell differentiation, the generation for promoting bud, but it is exposed under light and is easier to by light degradation be indoles and acetic acid, to lose
Its effect promotees the effect of differentiation, growth-promoting bud, therefore configuration facilitates storage life biological effectiveness under half-light, low temperature, simultaneously
The ethanol solution that (-) -8- phenylmenthol is added in proliferated culture medium can be effectively prevented the light degradation of heteroauxin,
Recombination reaction can promptly occur under the conditions of a small amount of (-) -8- phenylmenthol is existing for the ethyl alcohol with heteroauxin, it is raw
At (-) -8- phenylmenthol-heteroauxin compound can be used as a kind of stable preservation form of heteroauxin and enter and plant
In object, so as to avoid the light degradation of heteroauxin, under proper condition, plant is intracorporal should (-) -8- phenylmenthol -
Heteroauxin compound can dissociate heteroauxin and play biological effectiveness, induce the generation of axillary bud and adventitious bud, greatly
Ground promotes the quantity and quality of young shoot in Multiple Buds；
Prepare root media: sequentially added into MS culture medium 0.35mg/L KT, 32g/L sucrose, 18g/L coconut juice,
The tender willow juice of 2g/L Semen Coicis leachate, 9g/L, 8g/L agar, adjusting pH is 6.6, in 121 DEG C of high pressure sterilization 22min；Culture of rootage
Base can provide sufficient carbohydrate energy sources for the seedling by Multiplying culture, and Semen Coicis leachate can effectively be strengthened, regulate and control
The activity of the content of endogenous hormones and important enzyme in ase in Jasmine seedlings body, promotes the synthesis of large biological molecule, at the same induce main root and
The growth of lateral root adjusts metabolism degree, enhances the sturdy degree of seedlings root, reaches growth-promoting root, protects the effect of surviving, increases robust seedling seedling,
Nursery reproductive efficiency is greatly improved, economic benefit is expanded.
The acquisition methods of the Semen Coicis leachate are as follows: take SEMEN COICIS eluriate it is clean after as in the water of 2 times of weight, maceration
The vinegar of water weight 0.6% and 1% white sugar are added afterwards, stirring dissolves white sugar completely, and room temperature extracts 36 hours, filters to take
Filtrate is up to Semen Coicis leachate；Addition vinegar and white sugar facilitate the leaching of effective component in SEMEN COICIS, gained after Semen Coicis softening
To Semen Coicis leachate can effectively strengthen, regulate and control the activity of the content of endogenous hormones and important enzyme in ase in Jasmine seedlings body, promote
The synthesis of large biological molecule, while the growth of taproot and lateral root is induced, metabolism degree is adjusted, enhances the sturdy degree of seedlings root, reaches
To growth-promoting root, the effect of surviving is protected, increases robust seedling seedling, greatly improves nursery reproductive efficiency, expands economic benefit.
The acquisition methods of the tender willow juice are as follows: take current year raw tender withy, dissection after concentrating pressing soft, as soaking in water
It mentions, water had not had tender withy section just, persistently extracted 36 hours, took out withy section, removed insoluble matter up to tender willow juice.
Embodiment 4:
Jasmine, which quickly breeds, uses culture medium, including initial culture base, proliferated culture medium and root media,
The initial culture base are as follows: extracted containing 0.14mg/L KT, 8mg/L CTK, 24g/L sucrose, 15g/L lucid asparagus
The MS culture medium of liquid, 8g/L agar, pH 6.55；Preparation method are as follows: sequentially added into MS culture medium KT, CTK, sucrose,
Lucid asparagus extracting solution, agar adjust pH, in 121 DEG C of high pressure sterilization 20min to being completely dissolved；Initial culture base can meet jasmine
The nutritional need tentatively cultivated is organized, CTK can promote cell elongation and division, be ready for further Multiplying culture, day
Door winter extracting solution can effectively avoid jasmine tissue and browning, corruption occur, and promotes its histocyte smoothly to divide, break up, improves institute
The vigor and robustness of the tissue-cultured seedling of acquisition are conducive to subsequent Multiplying culture and culture of rootage；
The proliferated culture medium are as follows: thin containing 0.28mg/L KT, 5.5mg/L heteroauxin, 1.1mg/L (-) -8- phenyl
Lotus alcohol, 1.8g/L acetic acid, 26g/L sucrose, 4g/L agar MS culture medium, pH 6.6；Preparation method are as follows: toward MS culture medium
In sequentially add KT, (-) -8- phenylmenthol, acetic acid, sucrose, agar to being completely dissolved, adjust pH, go out in 121 DEG C of high pressures
Heteroauxin is added after cooling in 20min at a temperature of half-light, 1 DEG C, and half-light after dissolution saves at a temperature of 1 DEG C；It is trained in proliferation
The ethanol solution for supporting addition (-) -8- phenylmenthol in base can be effectively prevented the light degradation of heteroauxin, on a small quantity
Recombination reaction promptly can occur with heteroauxin under the conditions of (-) -8- phenylmenthol is existing for the ethyl alcohol, generation
(-) -8- phenylmenthol-heteroauxin compound can be used as a kind of stable preservation form of heteroauxin and enter plant
Interior, so as to avoid the light degradation of heteroauxin, under proper condition, plant is intracorporal should (-) -8- phenylmenthol-indoles
Acetic acid compound can dissociate heteroauxin and play biological effectiveness, induce the generation of axillary bud and adventitious bud, greatly mention
Rise the quantity and quality of young shoot in Multiple Buds；
The root media are as follows: containing 0.35mg/L KT, 30g/L sucrose, 20g/L coconut juice, 2g/L Semen Coicis leachate,
The MS culture medium of the tender willow juice of 10g/L, 8g/L agar, pH 6.7；Preparation method are as follows: sequentially added into MS culture medium KT,
Sucrose, coconut juice, Semen Coicis leachate, tender willow juice, agar adjust pH, in 121 DEG C of high pressure sterilization 20min；Root media can be
Sufficient carbohydrate energy sources are provided by the seedling of Multiplying culture, Semen Coicis leachate can effectively be strengthened, regulate and control jasmine children
The activity of the content of endogenous hormones and important enzyme in seedling body, promotes the synthesis of large biological molecule, while inducing taproot and lateral root
Growth adjusts metabolism degree, enhances the sturdy degree of seedlings root, reaches growth-promoting root, protects the effect of surviving, increases robust seedling seedling, mention significantly
High nursery reproductive efficiency expands economic benefit.
The acquisition methods of initial culture base Mid-Heaven Gate winter extracting solution are as follows: blend lucid asparagus spray tender leaf, according to solid-to-liquid ratio
Distilled water is added in 1:3, is stirred continuously 2.5 hours in 45 DEG C of temperature, filtering to take filtrate is lucid asparagus extracting solution；At high temperature
It stirs the effective component be conducive in lucid asparagus spray tender leaf to release, lucid asparagus extracting solution can effectively avoid jasmine tissue and occur
Browning, corruption promote its histocyte smoothly to divide, break up, and improve the vigor and robustness of acquired tissue-cultured seedling, are conducive to
Subsequent Multiplying culture and culture of rootage.
The acquisition methods of Semen Coicis leachate in root media are as follows: as 2.5 times of weight after taking SEMEN COICIS to eluriate completely
In water, the vinegar of water weight 1% and 1% white sugar are added after maceration, stirring dissolves white sugar completely, and room temperature extracts 48 hours,
Filtrate is filtered to take up to Semen Coicis leachate；Addition vinegar and white sugar facilitate the leaching of effective component in SEMEN COICIS after Semen Coicis softening
Out, the work of the content of endogenous hormones and important enzyme in ase in Jasmine seedlings body can effectively be strengthened, be regulated and controled to obtained Semen Coicis leachate
Property, promote the synthesis of large biological molecule, while inducing the growth of taproot and lateral root, adjust metabolism degree, enhancing seedlings root is thick
Strong degree reaches growth-promoting root, protects the effect of surviving, increase robust seedling seedling, greatly improve nursery reproductive efficiency, expands economic benefit.
The acquisition methods of the tender willow juice of root media are as follows: current year raw tender withy is taken, the soft rear dissection of pressing is concentrated, as
It is extracted in water, water had not had tender withy section just, persistently extracted 36 hours, took out withy section, removed insoluble matter up to tender willow juice.
Test case 5:
Jasmine quickly breeds, and peeling obtains jasmine seed embryo after jasmine flower seed is soaking, disinfection, successively presses
The following steps are cultivated and are transplanted:
Preliminary culture: embryo is inoculated in preliminary culture medium, cultivation temperature be 26 DEG C, light application time 12h, illumination
Intensity is 2500Lux, obtains tissue-cultured seedling after cultivating 25d；
Multiplying culture: the stem section that tissue-cultured seedling is chosen under aseptic condition is cut into segment according to 2~3 sections, is inserted into Multiplying culture
In base, cultivation temperature be 23 DEG C, light application time 10h, intensity of illumination 2200Lux, cultivate 25d after obtain Multiple Buds；
Culture of rootage: 1.5~3cm of clip has the stem section of bud from Multiple Buds under aseptic condition, is inserted into root media
In, cultivation temperature be 22 DEG C, light application time 10h, intensity of illumination 2800Lux, cultivate 30d after obtain rooted seedling；
Transplanting: it is transplanted after selecting rooted seedling hardening of fine quality.
The preliminary culture medium of difference, proliferated culture medium and root media successively in selection example 1~4, according to above-mentioned
Step carries out culture breeding to jasmine seed, detects rooted seedling when final acclimatization and transplants, and ocular estimate measures item number of averagely taking root
With rooting rate, vernier caliper method measures average root length, while detecting the seedling percent of transplanting after a week, and statistical data is such as
Shown in table 1.
The statistical data of rooted seedling in 1. Examples 1 to 4 of table
| Example is not | It averagely takes root item number (item) | Rooting rate | Average root length (cm) | Seedling percent |
| Embodiment 1 | 6.5 | 82.6% | 1.8 | 92.5% |
| Embodiment 2 | 18.6 | 98.5% | 3.0 | 97.8% |
| Embodiment 3 | 21.5 | 99.5% | 3.5 | 100% |
| Embodiment 4 | 22.5 | 100% | 3.8 | 100% |
As can be seen from Table 1, in example 4, seedling rooting rate and survival rate have reached 100%, and average
Item number of taking root has been more than 20, illustrate the method for the present invention have in terms of promoting root activity, rooting rate and seedling percent compared with
For positive effect.
The prior art of routine techniques dawn known to those skilled in the art in above-described embodiment, therefore herein no longer in detail
Carefully repeat.
The above embodiments are only used to illustrate the present invention, and not limitation of the present invention, the ordinary skill people of this field
Member can also make a variety of changes and modification without departing from the spirit and scope of the present invention.Therefore, all equivalent
Technical solution also belong to scope of the invention, scope of patent protection of the invention should be defined by the claims.
Claims (10)
1. a kind of Jasmine quickly breeds with culture medium, which is characterized in that including initial culture base, proliferated culture medium and training of taking root
Support base；
The initial culture base are as follows: containing 0.12~0.15mg/L KT, 5~10mg/L CTK, 22~25g/L sucrose, 12~
The MS culture medium of 15g/L lucid asparagus extracting solution, 8~10g/L agar, pH are 6.4~6.6；
The proliferated culture medium are as follows: containing 0.25~0.3mg/L KT, 5~6mg/L heteroauxin, 1.5~2g/L acetic acid, 25~
The MS culture medium of 28g/L sucrose, 3~5g/L agar, pH are 6.5~6.6；
The root media are as follows: contain 0.3~0.4mg/L KT, 30~32g/L sucrose, 15~25g/L coconut juice, 8~10g/L
The MS culture medium of tender willow juice, 4~10g/L agar, pH are 6.5~6.8.
2. a kind of Jasmine according to claim 1 quickly breeds with culture medium, which is characterized in that the proliferated culture medium
In also contain 1~1.2mg/L (-) -8- phenylmenthol.
3. a kind of Jasmine according to claim 1 quickly breeds with culture medium, which is characterized in that the root media
In also contain 1.5~3g/L Semen Coicis leachate.
4. a kind of Jasmine as described in claim 1 quickly breeds the preparation method with culture medium characterized by comprising
Preparation initial culture base: the KT, CTK, sucrose, lucid asparagus extracting solution, agar of formula ratio are sequentially added into MS culture medium
To being completely dissolved, pH, 121 DEG C of 15~25min of high pressure sterilization are adjusted；
It prepares proliferated culture medium: sequentially adding the KT of formula ratio, acetic acid, sucrose, agar into MS culture medium to being completely dissolved, adjust
PH is saved, in 121 DEG C of 15~25min of high pressure sterilization；After cooling, 5~6mg/L heteroauxin is added at a temperature of half-light, 1~2 DEG C,
Half-light after dissolution saves at a temperature of 1~2 DEG C；
It prepares root media: sequentially adding KT, sucrose, coconut juice, the tender willow juice, agar of formula ratio into MS culture medium, adjust
PH, in 121 DEG C of 15~25min of high pressure sterilization.
5. a kind of Jasmine according to claim 4 quickly breeds the preparation method with culture medium, which is characterized in that described
(-) -8- phenylmenthol of 1~1.2mg/L is also added into formula ratio in proliferated culture medium.
6. a kind of Jasmine according to claim 4 quickly breeds the preparation method with culture medium, which is characterized in that described
The Semen Coicis leachate of 1.5~3g/L is also added into formula ratio in root media.
7. a kind of Jasmine according to claim 6 quickly breeds the preparation method with culture medium, which is characterized in that described
State the acquisition methods of Semen Coicis leachate are as follows: as in the water of 2~3 times of weight after taking SEMEN COICIS to eluriate completely, food is added after maceration
Vinegar and white sugar, stirring dissolve white sugar completely, and room temperature extracts 36~48 hours, filter to take filtrate up to Semen Coicis leachate.
8. a kind of Jasmine according to claim 7 quickly breeds the preparation method with culture medium, which is characterized in that described
In the acquisition methods for stating Semen Coicis leachate, the additive amount of vinegar is the 0.5~1% of water weight, and the additive amount of white sugar is water weight
1%.
9. a kind of Jasmine according to claim 4 quickly breeds the preparation method with culture medium, which is characterized in that described
The acquisition methods of lucid asparagus extracting solution are as follows: lucid asparagus spray tender leaf is blended, distilled water is added according to solid-to-liquid ratio 1:2.5~4,
45~48 DEG C of temperature are stirred continuously 2~3 hours, and filtering to take filtrate is lucid asparagus extracting solution.
10. quickly application of the breeding culture medium in Jasmine quickly breeds of the described in any item Jasmines of claim 1-9.
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| WO2022024091A1 (en) * | 2020-07-30 | 2022-02-03 | Rothwell Victoria | Plant rooting composition and uses thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2022024091A1 (en) * | 2020-07-30 | 2022-02-03 | Rothwell Victoria | Plant rooting composition and uses thereof |
| GB2612753A (en) * | 2020-07-30 | 2023-05-10 | Rothwel Victoria | Plant rooting composition and uses thereof |
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