CN101805721A - Method for culturing corn haploid coleoptile section tissue and specific culture medium thereof - Google Patents
Method for culturing corn haploid coleoptile section tissue and specific culture medium thereof Download PDFInfo
- Publication number
- CN101805721A CN101805721A CN 201010139732 CN201010139732A CN101805721A CN 101805721 A CN101805721 A CN 101805721A CN 201010139732 CN201010139732 CN 201010139732 CN 201010139732 A CN201010139732 A CN 201010139732A CN 101805721 A CN101805721 A CN 101805721A
- Authority
- CN
- China
- Prior art keywords
- final concentration
- monoploid
- proline
- culture medium
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a method for culturing corn haploid coleoptile section tissue and a specific culture medium thereof. The differentiation culture medium provided by the invention is the culture medium used in the differentiation and shoot formation of corn haploid coleoptile section induced callus, and is prepared by adding the following substances into MS basic culture medium: proline, casein hydrolyzate, carbon and gel, wherein the final concentration of the proline is 0.7g/L, and the final concentration of the casein hydrolyzat is 0.5g/L. The method of the invention adopts corn coleoptile section as the explant induced callus, and then differentiates the callus into a haploid strain to establish a stable and high-efficiency tissue culture system. The invention can be used for differentiation rate genetic research, material selection for tissue culture, and the like.
Description
Technical field
The present invention relates to plant tissue culture and crop breeding field, particularly corn haploid bud scale joint tissue culture and doubling monoploids and special culture media.
Background technology
Corn (Zea mays L.) also claim Zea mays, maize, maize, ear of maize; Guangdong language is called maize, and the south of Fujian Province language is called a kind wheat, is the annual gramineae herbaceous plant, also is the highest food crop of whole world ultimate production.Class of trade is mainly divided according to the quality of seed, is divided into horse tooth kind, hard kind, powder seed, explosion kind and Glutinous Semen Maydis, sweet corn etc.Seed top depression causes because of the ridity of hard starch of seed and soft starch is unequal.It is few that flint corn contains soft starch, dry back top depression.Flour corn mainly contains soft starch, and opaque easily pulverizes.Sweet corn is sent out wrinkle, and is transparent, and sugar is not converted into starch.Pop corn is the extreme form of jadeite rice, and seed is little and hard, does not contain soft starch, and ICW expands during heating, the seed explosion.Can improve the corn type with good hybridization between selfed lines.
Corn is as feed, food and industrial raw material, at the main food of many areas conduct.Except that edible, corn also is the main raw material of industrial spirit and liquor.The other parts purposes of milpa is also quite extensive: cornstalk is used for papermaking and system wallboard; Foreskin can be done packing material and the braiding of grass skill; Corn cob can be made fuel, also is used for preparing industrial solvent, and cauline leaf is except that being used as animal feed, or the methane-generating pit good raw material.
Utilize haploid breeding can overcome hybrid and separate, shortening the breeding cycle, improve selection rate.By obtaining haplobiont, pass through chromosome doubling again, can obtain the diploid of isozygotying very soon, like this general shortening the breeding cycle greatly.
Corn haploid bud scale joint tissue culture obtains the technology of haplobiont, and not seeing at present has report.
Summary of the invention
The object of the present invention is to provide a kind of special culture media that is used for corn haploid bud scale joint callus differentiation culture.
Division culture medium provided by the invention is to add the solid medium that following material obtains in the MS basic culture solution: proline(Pro), casein hydrolysate, carbon source and gelifying agent;
The solvent of above-mentioned MS basic culture solution is that water, solute are as shown in table 1;
The final concentration of proline(Pro) is that the final concentration of 0.6-0.8g/L, casein hydrolysate is 0.4-0.6g/L in the above-mentioned division culture medium.
The solute of table 1.MS basic culture solution
| Macroelement | Concentration (gL in the substratum -1) |
| ??NH 4NO 3 | ??1.65 |
| ??KNO 3 | ??1.9 |
| ??KH 2PO 4 | ??0.17 |
| ??MgSO 4.7H 2O | ??0.37 |
| ??CaCl 2 | ??0.44 |
| Trace element | Concentration (mgL in the substratum -1) |
| ??FeSO 4.7H 2O | ??27.8 |
| ??Na 2EDTA | ??37.3 |
| ??MnSO 4.4H 2O | ??22.3 |
| ??ZnSO 4.4H 2O | ??8.6 |
| ??H 3BO 3 | ??6.2 |
| ??KI | ??0.83 |
| ??Na 2MoO 4.2H 2O | ??0.25 |
| ??CuSO 4.5H 2O | ??0.025 |
| ??CoCl 2.6H 2O | ??0.025 |
| Organic composition | Concentration (mgL in the substratum -1) |
| Glycine | ??2.0 |
| Vitamin | ??0.4 |
| Pyridoxine hydrochloride | ??0.5 |
| Nicotinic acid | ??0.5 |
| Inositol | ??100 |
Above-mentioned gelifying agent is tissue culture such as agar powder, carrageenin or Gelrite solidifying agent commonly used, Gelrite preferably, the substratum hardness of the consumption of Gelrite (gelatin) reaching can suitably be adjusted consumption, and the concentration of Gelrite in division culture medium specifically can be 3.0g/L.
Above-mentioned carbon source is glucose, maltose or sucrose, is preferably sucrose, and the concentration of sucrose in division culture medium specifically can be 30g/L.
Further, in the above-mentioned division culture medium final concentration of proline(Pro) and casein hydrolysate preferably proline(Pro) be that 0.7g/L, casein hydrolysate are 0.5g/L.
The present invention also aims to provide a kind of method of producing the corn haplobiont.
The invention provides the method for producing corn monoploid regeneration plant, may further comprise the steps:
1) mature embryo with the monoploid corn kernel places the MSVS34 substratum to cultivate, and sprouts to obtain plumule, chooses colourless monoploid plumule;
2) with above-mentioned steps 1) in the monoploid plumule that obtains cut the coleoptile joint and be inoculated in the MSW57 callus inducing medium and cultivate, obtain the monoploid callus;
3) with above-mentioned steps 2) in the monoploid callus that obtains place above-mentioned division culture medium to cultivate, obtain the monoploid regeneration plant.
Above-mentioned MSVS34 substratum and MSW57 callus inducing medium all are substratum commonly used in the plant tissue culture.
The method of production corn haplobiont of the present invention is in described step 2) and step 3) between also can comprise succeeding transfer culture, described succeeding transfer culture is with described step 2) in the monoploid callus that obtains place the N6 subculture medium to cultivate propagation, the monoploid callus that obtains breeding.
Above-mentioned N6 subculture medium is to add casein hydrolysate, proline(Pro), 2, the solid medium that 4-D, carbon source and gelifying agent obtain in the N6 basic culture solution; Described carbon source is preferably sucrose, and gelifying agent is preferably Gelrite.The final concentration of described sucrose in described N6 subculture medium is 30.0g/L, and the final concentration of described Gelrite in described N6 subculture medium is 2.7g/L
The solvent of described N6 basic culture solution is that water, solute are as shown in table 2; Wherein casein hydrolysate, proline(Pro) and 2, the final concentration of 4-D is respectively following 1) or 2):
1) casein hydrolysate is 0.5g/L, and proline(Pro) is 0.7g/L, 2, and 4-D is 1.5-2.5mg/L;
2) casein hydrolysate is 0.5g/L, and proline(Pro) is 0.7g/L, 2, and 4-D is 2mg/L.
Casein hydrolysate, proline(Pro) and 2 in the optimum N6 subculture medium, the final concentration of 4-D is above-mentioned 2) described.
The solute of table 2.N6 basic culture solution
| Macroelement | Concentration (gL in the substratum -1) |
| ??KNO 3 | ??2.83 |
| ??(NH 4) 2SO 4 | ??0.46 |
| ??KH 2PO 4 | ??0.4 |
| ??MgSO 4.7H 2O | ??0.185 |
| ??CaCl 2.2H 2O | ??0.166 |
| Macroelement | Concentration (gL in the substratum -1) |
| Trace element | Concentration (mgL in the substratum -1) |
| ??FeSO 4.7H 2O | ??27.8 |
| ??Na 2EDTA | ??37.3 |
| ??MnSO 4.4H 2O | ??4.4 |
| ??ZnSO 4.7H 2O | ??1.5 |
| ??H 3BO 3 | ??1.6 |
| ??KI | ??0.8 |
| Organic composition | Concentration (mgL in the substratum -1) |
| Glycine | ??2.0 |
| Vitamin | ??1.0 |
| Pyridoxine hydrochloride | ??0.5 |
| Nicotinic acid | ??0.5 |
The method of above-mentioned production corn haplobiont also can comprise: the haplobiont that obtains in the step 3) is inoculated into carries out root culture, the haplobiont that obtains taking root in the root media.
Above-mentioned root media is to add the solid medium that NAA, carbon source and gelifying agent obtain in the MS basic culture solution;
The solvent of described MS basic culture solution is that water, solute are as shown in table 1; Described carbon source is preferably sucrose, and gelifying agent is preferably Gelrite.The final concentration of described sucrose in described root media is 30g/L, and the final concentration of described Gelrite in described root media is 1.2g/L.Wherein the final concentration of NAA is 0.25-0.7mg/L, preferably 0.5mg/L.
Division culture medium of the present invention can be divided into plant with maize bud scale joint inductive callus effectively, and the differentiation adventitious buds rate reaches 36%, and after cultivating through root media, rooting rate reaches 88.9%.
Method of the present invention is to utilize the coleoptile joint of corn haploid bud differentiation obtains haplobiont as the explant induction callus, the tissue culturing system that the present invention sets up can be used for haploid breeding research effectively, for keeping good monoploid material, and good monoploid material is doubled as zygoid good material is provided.
Description of drawings
Fig. 1 is a corn monoploid seed.
The monoploid plumule that Fig. 2 sprouts for mature embryo of the present invention.
Fig. 3 is the present invention's haploid embryo bud scale joint to be inoculated.
Fig. 4 induces the monoploid callus that obtains for haploid embryo bud scale joint.
Fig. 5 is the monoploid callus of subculture.
The monoploid regeneration plant that Fig. 6 obtains for the callus induction differentiation.
The haplobiont that Fig. 7 survives for rooting and transplant.
Embodiment
The invention will be further described below in conjunction with specific embodiment, but the present invention is not limited to following examples.
Among the following embodiment, if no special instructions, be ordinary method.
The preparation of embodiment 1, corn haplobiont and statistical observation
One, the acquisition of monoploid seed
, hybridize as male parent with corn haploid inducing line Stock6 (U.S. profound scholar gets company, Garst Seed Co) with agricultural university 108 (purchase) in the greatly healthy scientific and technological development of Beijing middle peasant company limited.After seed maturity, the drying, select the seed of the white embryo in purple top, standby.
Two, the acquisition of monoploid plumule and statistical observation
1, the pre-treatment of monoploid seed
The purple that from step 1, obtains push up white embryo corn kernel (be corn monoploid seed, select 100 in as shown in Figure 1), the envelope of packing into, opening is put into moisture eliminator with envelope.Use the beaker of 250mL, contain 200mL 5.25% (percent by volume) chlorine bleach liquor, in beaker, add the 2mL concentrated hydrochloric acid again, put into moisture eliminator central authorities, encapsulation process 8-15 hour.Seed is taken out, put into beaker, in Bechtop, add 70% (percent by volume) Ethanol Treatment 1min; Remove 70% ethanol, add 2% (percent by volume) chlorine bleach liquor, soak 20min; With aseptic washing 4-5 time, use the sterilized water soaked overnight at last.
2, obtain the monoploid plumule
On super clean bench, the seed of soaked overnight is peeled off, obtain mature embryo, be inoculated in the MSVS34 substratum, place in the illumination cultivation chamber and cultivated 7-10 days, culture condition is in the illumination cultivation chamber: place 25 ℃ of illumination cultivation, intensity of illumination 2000lx, illumination every day 16h cultivates 7-10d.Plumule after obtaining sprouting is rejected the plumule material that radicle, embryo or plumule are purple, chooses acquisition radicle, embryo and plumule and is colourless monoploid plumule (as shown in Figure 2) material.
Above-mentioned MSVS34 substratum is to add the substratum that following material obtains in the MS basic culture solution: casein hydrolysate, MES (2-morpholinoethanesulfonic acid), magnesium chloride, glutamine, xitix, picloram (picloram), BAP (benzyladenine), carbon source and gelifying agent; The solvent of MS basic culture solution is that water, solute are as shown in table 1; Wherein the final concentration of casein hydrolysate is 0.1g/L, the final concentration of MES is 1.95g/L, the final concentration of magnesium chloride is 0.75g/L, the final concentration of glutamine is 0.5g/L, the final concentration of xitix is 0.1g/L, and the final concentration of picloram is 10mg/L, and the final concentration of BAP is 3mg/L, above-mentioned carbon source is a maltose, and gelifying agent is an agar; The final concentration of maltose is 40g/L, and the final concentration of agar is 8g/L, can suitably adjust its consumption, and the pH of MSVS34 substratum is 5.8.
3, statistical observation
Experiment repeats 3 times, and the MSVS34 substratum is induced the result of mature embryo sprouting and the yield of monoploid plumule in the statistical observation above-mentioned steps 2.Experimental result sees the following form 3.As can be seen from Table 3, after mature embryo was sprouted, rejecting radicle, plumule were the material of purple, and keeping radicle and plumule is the monoploid material of non-purple.
Table 3.MSVS34 substratum is induced mature embryo sprouting situation statistics
Three, the acquisition of callus and statistical observation
1, the acquisition of callus
When the monoploid plumule for the treatment of in the above-mentioned steps two grows to about 5-6cm monoploid seedling, cut coleoptile joint (upper and lower each 0.5cm of stipes of about 1em, as shown in Figure 3), and use the scalpel five equilibrium, tangent plane is close to substratum, be inoculated in the MSW57 callus inducing medium, 25 ℃ of illumination cultivation, intensity of illumination 2000lx, illumination every day 16h, illumination cultivation 21d obtains monoploid callus (as shown in Figure 4).
Above-mentioned MSW57 callus inducing medium is to add vitamin, casamino acids, proline(Pro), Silver Nitrate, 2, the substratum that 4-D, picloram, carbon source and gelifying agent obtain in the MS basic culture solution; The solvent of MS basic culture solution is that water, solute are as shown in table 1; Wherein the final concentration of vitamin is 0.9mg/L, and the final concentration of casamino acids is 0.5g/L, and the final concentration of proline(Pro) is 1.38g/L, and the final concentration of Silver Nitrate is 3.4mg/L, 2, and the final concentration of 4-D is 0.5mg/L, the final concentration of picloram is 2.2mg/L; Above-mentioned carbon source is a sucrose, and gelifying agent is an agar; The final concentration of sucrose is 30g/L, and the final concentration of agar is 8g/L, and the pH of substratum is 5.8.
2, statistical observation
Experiment repeats 3 times, in the statistical observation above-mentioned steps 1 callus induce situation, experimental result sees the following form 4, the inductivity of callus can reach more than 36.9%.
Table 4. callus induction rate
Four, the succeeding transfer culture of callus and statistical observation
1, succeeding transfer culture
The callus that obtains in the step 3 is transferred in the N6 subculture medium, is under 25 ℃ the condition in temperature, the dark cultivation, and per 21 days subcultures once obtain the monoploid callus (as shown in Figure 5) of required propagation quantity.
Above-mentioned N6 subculture medium is to add the substratum that following material obtains in the N6 basic culture solution: casein hydrolysate, proline(Pro), 2,4-D, carbon source and gelifying agent; Wherein carbon source is a sucrose, and the final concentration of sucrose in substratum is 30g/L, and gelifying agent is Gelrite, and the final concentration of Gelrite is 2.7g/L, and the final concentration of casein hydrolysate is 0.5g/L, and the final concentration of proline(Pro) is 0.7g/L, 2, and the final concentration of 4-D is 2mg/L; The pH of substratum is 5.8, and the solute of N6 basic culture solution is as shown in table 2, and its solvent is a water.
Five, the acquisition of monoploid regeneration plant and statistical observation
1, obtains the monoploid regeneration plant
The monoploid callus that succeeding transfer culture obtains in monoploid callus that above-mentioned steps three is obtained or the step 4 is cut into small pieces and changes in the division culture medium, illumination cultivation, culture condition is, temperature is 25 ℃, be placed under the intensity of illumination of 2000lx and cultivate, every day, light was cultivated 16 hours, secretly cultivated 8 hours, and differentiation obtains monoploid regeneration plant (as shown in Figure 6).
Above-mentioned division culture medium is to add the substratum that proline(Pro), casein hydrolysate, carbon source and gelifying agent obtain in the MS basic culture solution, and the solvent of MS basic culture solution is that water, solute are as shown in table 1; Wherein carbon source is that final concentration is the sucrose of 30g/L, and gelifying agent is Gelrite, and the final concentration of Gelrite is 3.0g/L, and the final concentration of proline(Pro) is 0.7g/L, and the final concentration of casein hydrolysate is 0.5g/L, and the pH of substratum is 5.8.
2, statistical observation
Experiment repeats 3 times, the situation of regeneration differentiation in the statistical observation above-mentioned steps 1, and experimental result sees the following form 5, and as can be seen from Table 5, the differentiation of calli rate can reach more than 54%, and the differentiation rate of indefinite bud reaches more than 22%.
The differentiation rate of table 5. regeneration differentiation culture
Six, root culture and statistical observation
1, the monoploid acquisition of plant of taking root
When the haplobiont that obtains in the above-mentioned steps five is grown to 3-5cm, change over to and carry out root culture in the root media, culture condition is 25 ℃ of illumination cultivation, intensity of illumination 2000lx, illumination every day 16h cultivated about 21 days, the good monoploid that obtains the taking root plant that takes root.
Above-mentioned root media is to add the substratum that NAA, carbon source and gelifying agent obtain in the MS basic culture solution, and the solvent of MS basic culture solution is that water, solute are as shown in table 1; Wherein carbon source is that final concentration is the sucrose of 30g/L, and gelifying agent is Gelrite, and the final concentration of Gelrite is 1.2g/L, and the final concentration of NAA is 0.5mg/L, and the pH of substratum is 5.8.
2, statistical observation
Experiment repeats 3 times, the root culture situation of statistical observation above-mentioned steps 1, and experimental result sees the following form 6, and rooting rate reaches more than 81.7%, and every strain haplobiont is on average taken root 2.1.
The rooting rate of table 6. root culture
Seven, acclimatization and transplants and check and analysis
1, acclimatization and transplants
The good monoploid that takes root in the above-mentioned steps six is taken root after the plant hardening, transplant to flowerpot, growth is 7-10 days in the greenhouse, and the plant of transplant survival is planted in the greenhouse, and the haplobiont that obtains transplant survival (is the regenerated plant, as shown in Figure 7).The surviving rate of transplanting is 75%.
2, check and analysis
When regeneration plant in the above-mentioned steps 1 (be monoploid take root plant) is transplanted flowerpot, cut 1-2 the tip of a root, usefulness Kano stationary liquid (dehydrated alcohol: Glacial acetic acid=3: 1) fixedly more than the 2h.Use ddH
2O adds an amount of cellulase and polygalacturonase mixture enzymolysis 2h after cleaning for several times.Compressing tablet is examined under a microscope chromosome number, found that chromosome number is 10 all, illustrates that the regenerated plant all is a haplobiont.
Claims (10)
1. a division culture medium is to add the solid medium that following material obtains in the MS basic culture solution: proline(Pro), casein hydrolysate, carbon source and gelifying agent;
The solvent of described MS basic culture solution is that water, solute are as shown in table 1;
The final concentration of proline(Pro) is that the final concentration of 0.6-0.8g/L, casein hydrolysate is 0.4-0.6g/L in the described division culture medium.
2. division culture medium according to claim 1 is characterized in that: described gelifying agent is agar powder, carrageenin or Gelrite, Gelrite preferably, and wherein the final concentration of Gelrite in described division culture medium is 3.0g/L; Described carbon source is glucose, maltose or sucrose, is preferably sucrose, and wherein the final concentration of sucrose in described division culture medium is 30g/L.
3. division culture medium according to claim 2 is characterized in that: the final concentration of proline(Pro) and casein hydrolysate is respectively in the described division culture medium: proline(Pro) is that 0.7g/L, casein hydrolysate are 0.5g/L.
4. method of producing corn monoploid regeneration plant may further comprise the steps:
1) mature embryo with the monoploid corn kernel places the MSVS34 substratum to cultivate, and sprouts to obtain plumule, chooses colourless monoploid plumule;
2) with above-mentioned steps 1) in the monoploid plumule that obtains cut the coleoptile joint and be inoculated in the MSW57 callus inducing medium and cultivate, obtain the monoploid callus;
3) with above-mentioned steps 2) in the monoploid callus that obtains place the arbitrary described division culture medium of claim 1-3 to cultivate, obtain the monoploid regeneration plant.
5. method according to claim 4, it is characterized in that: the method for described production corn haplobiont is in described step 2) and step 3) between also comprise succeeding transfer culture, described succeeding transfer culture is with described step 2) in the monoploid callus that obtains place the N6 subculture medium to cultivate propagation, the monoploid callus that obtains breeding.
6. method according to claim 5 is characterized in that: described N6 subculture medium is to add casein hydrolysate, proline(Pro), 2, the solid medium that 4-D, carbon source and gelifying agent obtain in the N6 basic culture solution;
Described carbon source is sucrose preferably, and described gelifying agent is Gelrite preferably;
The solvent of described N6 basic culture solution is that water, solute are as shown in table 2;
Wherein casein hydrolysate, proline(Pro) and 2, the final concentration of 4-D is respectively following 1) or 2):
1) casein hydrolysate is 0.5g/L, and proline(Pro) is 0.7g/L, 2, and 4-D is 1.5-2.5mg/L;
2) casein hydrolysate is 0.5g/L, and proline(Pro) is 0.7g/L, 2, and 4-D is 2mg/L.
7. method according to claim 6 is characterized in that: the final concentration of described sucrose in described N6 subculture medium is 30.0g/L, and the final concentration of described Gelrite in described N6 subculture medium is 2.7g/L.
8. according to arbitrary described method among the claim 4-7, it is characterized in that: the method for described production corn haplobiont also comprises: the haplobiont that obtains in the step 3) is inoculated into carries out root culture, the haplobiont that obtains taking root in the root media.
9. method according to claim 8 is characterized in that: described root media is to add the solid medium that NAA, carbon source and gelifying agent obtain in the MS basic culture solution;
The solvent of described MS basic culture solution is that water, solute are as shown in table 1;
Wherein the final concentration of NAA is 0.25-0.7mg/L, preferably 0.5mg/L.
10. method according to claim 9 is characterized in that: the carbon source in the described root media is a sucrose, and gelifying agent is Gelrite; The final concentration of described sucrose in described root media is 30g/L, and the final concentration of described Gelrite in described root media is 1.2g/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101397320A CN101805721B (en) | 2010-04-01 | 2010-04-01 | Method for culturing corn haploid coleoptile section tissue and specific culture medium thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101397320A CN101805721B (en) | 2010-04-01 | 2010-04-01 | Method for culturing corn haploid coleoptile section tissue and specific culture medium thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101805721A true CN101805721A (en) | 2010-08-18 |
| CN101805721B CN101805721B (en) | 2012-05-02 |
Family
ID=42607675
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010101397320A Active CN101805721B (en) | 2010-04-01 | 2010-04-01 | Method for culturing corn haploid coleoptile section tissue and specific culture medium thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101805721B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103173487A (en) * | 2013-04-23 | 2013-06-26 | 北京金冠丰生物技术有限公司 | Anniversary large-scale maize transformation method |
| CN103190346A (en) * | 2013-04-22 | 2013-07-10 | 北京金冠丰生物技术有限公司 | Method for constructing corn reproduction system taking coleoptile section as explant |
| CN104026017A (en) * | 2014-06-20 | 2014-09-10 | 四川农业大学 | Culture method of corn haplobiont |
| CN108368518A (en) * | 2015-10-02 | 2018-08-03 | 主基因有限公司 | The method for preparing monoploid and subsequent doubled haploid plant |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101011031A (en) * | 2007-02-07 | 2007-08-08 | 华中农业大学 | Callus induction and plant regeneration method by corn mature embryo approach |
| US20080274547A1 (en) * | 2003-12-19 | 2008-11-06 | Monsanto Technology Llc | Use of nitric oxide modulators in agrobacterium-mediated plant transformation |
-
2010
- 2010-04-01 CN CN2010101397320A patent/CN101805721B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080274547A1 (en) * | 2003-12-19 | 2008-11-06 | Monsanto Technology Llc | Use of nitric oxide modulators in agrobacterium-mediated plant transformation |
| CN101011031A (en) * | 2007-02-07 | 2007-08-08 | 华中农业大学 | Callus induction and plant regeneration method by corn mature embryo approach |
Non-Patent Citations (1)
| Title |
|---|
| 《Plant Cell Rep》 20051027 Vladimir Sidorov et al Agrobacterium-mediated transformation of seedling-derived maize callus 320-328 1-10 第25卷, 第4期 2 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103190346A (en) * | 2013-04-22 | 2013-07-10 | 北京金冠丰生物技术有限公司 | Method for constructing corn reproduction system taking coleoptile section as explant |
| CN103173487A (en) * | 2013-04-23 | 2013-06-26 | 北京金冠丰生物技术有限公司 | Anniversary large-scale maize transformation method |
| CN104026017A (en) * | 2014-06-20 | 2014-09-10 | 四川农业大学 | Culture method of corn haplobiont |
| CN108368518A (en) * | 2015-10-02 | 2018-08-03 | 主基因有限公司 | The method for preparing monoploid and subsequent doubled haploid plant |
| CN108368518B (en) * | 2015-10-02 | 2023-12-05 | 主基因有限公司 | Method for producing haploid and subsequent doubled haploid plants |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101805721B (en) | 2012-05-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101611697B (en) | Virus removal and rapid propagation technology of sweet potato variety 'Shangshu 19' | |
| CN101779598B (en) | Method for building high-efficiency regeneration system of superior corn self-bred line agriculture line 531 | |
| CN111492973A (en) | Method for obtaining regeneration plants from common camellia oleifera through somatic embryogenesis | |
| CN103416304A (en) | Method for cultivating water-saving and drought-resistant rice anther | |
| CN101805721B (en) | Method for culturing corn haploid coleoptile section tissue and specific culture medium thereof | |
| CN101548646B (en) | Method for rapidly propagating aralia elata through somatic embryo and secondary somatic embryogenesis | |
| CN103039360B (en) | Method for quickly propagating leeka through tissue culture | |
| Hegele et al. | Low-cost gelling agents for tissue culture propagation of plantain | |
| CN1203754C (en) | Breeding technology of test-tube konjac | |
| CN101233825A (en) | Edible lily detoxifying fast breeding culture medium | |
| CN101904302B (en) | Method for somatic cell embryogeny and plant regeneration of medicinal plant schisandga chinensis baill | |
| Burbulis et al. | Influence of genotype, growth regulators, sucrose level and preconditioning of donor plants on flax (Linum usitatissimum L.) anther culture | |
| CN110199884A (en) | A kind of breeding method of setting seeds under quinoa tissue-cultured seedling aseptic condition | |
| CN113575422B (en) | Efficient in-vitro regeneration method of pineapple leaves | |
| CN100362096C (en) | Method for obtaining amphihaploid plant of pumpkin ovule and its special culture medium | |
| KR101439618B1 (en) | A Method for Mass Propagation of Rhododendron Keiskei var. hypoglaucum by Plant Tissue Culture | |
| CN108552059B (en) | Plant tissue culture method for promoting strong roots of potato seedlings | |
| Feyisa | Micropropagation of Cassava (Manihot esculenta Crantz) | |
| CN106538381B (en) | A kind of plant establishment strong seedling culture base and preparation method and application containing paclobutrazol | |
| Berhanu et al. | Factors Affecting in Vitro Propagation of Cassava (Manihot Esculenta Crantz.) Euphorbiaceae, Varieties of ‘Kello’and ‘Qulle | |
| Cai et al. | Optimization of a rapid propagation system for mass production of high-quality plantlets of Trichosanthes kirilowii cv.‘Yuelou-2’via organogenesis | |
| Heidari et al. | Efficient androgenic embryo induction and plant regeneration in different genotypes of sweet pepper via anther culture | |
| Dash et al. | Effect of Thermo-Stress on Morpho-Phenological and Reproductive Behaviour of Groundnut (Arachis hypogaea L.) | |
| CN109329051A (en) | Jasmine quickly breeds the preparation method with culture medium | |
| Hoque et al. | Variation of callus induction through anther culture in water chestnut (Trapa sp.) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |