CN106538381B - A kind of plant establishment strong seedling culture base and preparation method and application containing paclobutrazol - Google Patents
A kind of plant establishment strong seedling culture base and preparation method and application containing paclobutrazol Download PDFInfo
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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Abstract
The present invention relates to a kind of plant establishment strong seedling culture base and preparation method and application containing paclobutrazol.The present invention is by studying effect of the different additives to plant test tube seedling, filter out a kind of efficient Rooting and hardening-off culture base, the plant establishment strong seedling culture base is to add paclobutrazol in root media and be made, wherein the concentration of paclobutrazol is 0.3 2.5mg/L, the culture medium can be such that test tube seedling is compared with control, not only seedling becomes short strong, dark green leaf color, and it is fast to take root, root is thicker and root hair increase it is elongated, after transplanting, seedling turns green soon, growing way is good, so as to improve the transplanting survival rate of plant test tube seedling, the final efficiency for improving research gene function and tissue culture breeding, had a good application prospect in plant genetic engineering field.
Description
Technical field
The present invention relates to a kind of plant establishment strong seedling culture base and its preparation method and application, belong to Plant Tissue Breeding and
Field of transgenic technology.
Background technology
Plant tissue culture technique is the technology to grow up early 20th century, is referred to aseptically, by vitro plant
Sundries official, tissue, cell, protoplast etc. carry out cultured in vitro in suitable condition, and induction produces callus, adventitious bud
Deng, afterwards formed intact plant process.Tissue culture technique can utilize the control of various culture conditions thin because it has
The advantages that growth of born of the same parents, differentiation, plant physiology, pathology, pharmacy, science of heredity, breeding and bioid are effectively promoted
The cross development of etc. subject, and it is widely used in a variety of industries such as agricultural, forestry, gardening, industry, pharmaceutical sector, produce huge
Economic benefit and social benefit, be summed up, there is the following aspects：1st, the rapid propagation in vitro of nursery stock；2nd, detoxic seedling is cultivated；3、
Culture medium transgenic breeding applied to plant breeding such as haploid breeding, protoplast fusion, embryo and endosperm etc.；4th, production time
Raw metabolite such as traditional Chinese medicine ingredients etc.；5th, artificial seed and preserving seed；6th, gene functional research etc..
Under suitable isolated culture condition, the cell of organizing specific, again division formation possessed all-round originally
Property cell mass, i.e. callus, this process is referred to as " dedifferentiation "；Totipotent cell through " dedifferentiation ", re-form root, bud
And the process of all kinds cell such as conducting system is referred to as " breaking up again ".Dedifferentiation and differentiation are the important of Plant Tissue Breeding
Link, decisive influence is played to tissue cultures efficiency.In addition, the test tube seedling that tissue cultures and genetic transformation are obtained is past
Weaker toward growing way, root system is undeveloped, and survival rate is low after transplanting, and the culture efficiency and further application for having a strong impact on test tube seedling are imitated
Fruit.Therefore, a kind of efficient plant establishment strong sprout system is established, is just particularly important.
Paclobutrazol (Paclobutrazol) is a kind of triazole type plant growth regulator, is the suppression of endogenous gibberellins synthesis
Preparation, the activity of rice indoleacetic acid oxidase can be improved, reduce the level of rice seedling Endogenous IAA.With plant growth is delayed, press down
Cane elongation processed, shortens internode；Weaken rice seedling apical growth advantage, promote lateral bud (tiller) to grow, promote bud differentiation；Increase
Plant stress-resistance performance, improve yield and other effects.Paclobutrazol can be by seed, leaf, root absorption, after spraying paclobutrazol, outside rice shoot
The short strong more tillers of performance are seen, leaf color is dark green, well developed root system.
The content of the invention
It is an object of the present invention to provide a kind of plant establishment strong seedling culture base.The present invention is by exploring different additives to planting
The effect of thing test tube seedling, a kind of Rooting and hardening-off culture base is filtered out, makes plant seedlings and contrast ratio, not only seedling becomes short strong and leaf
Color depth is green, and fast, thicker and root hair of taking root increases elongated, and after transplanting, seedling turns green soon, and growing way is good, so as to improve plant test tube seedling
Transplanting survival rate, the final efficiency for improving research gene function and tissue culture breeding.
Plant establishment strong seedling culture base provided by the invention, it is the root media containing paclobutrazol.
The root media includes but is not limited to MS culture mediums, N6 culture mediums or B5 medium etc., the one of the present invention
In individual embodiment, the root media used is 1/2MS culture medium.The root media of the present invention includes but is not limited to 1/2MS
Culture medium.1/2MS basal mediums described in the embodiment of the present invention is term generally in the art, is commercially available, also can be voluntarily
Prepare, its formula is common knowledge.
In the plant establishment strong seedling culture base of the present invention, the concentration of paclobutrazol is 0.3-2.5mg/L.
Preferably, the final concentration of 0.4-1.5mg/L of paclobutrazol.
It is highly preferred that the final concentration of 0.5-1mg/L of paclobutrazol.
Further, plant establishment strong seedling culture base of the invention also contains sugar, and the sugar is in the plant establishment
Concentration in strong seedling culture base is 15-25g/L, preferably 20g/L.
The plant establishment strong seedling culture base pH value of the present invention is 5.7-6.0.The plant establishment strong seedling culture base of the present invention is also
Containing coagulator, preferable coagulator is Phytagel plant gels or agar powder.Phytagel plant gels are from false unit cell
A kind of substitute of agar of bacterium secretion, also known as Gelrite, Geuam Gum etc..It can be obtained from commercially available, such as SIGMA public
Department.
Concentration of the Phytagel plant gels in the Rooting and hardening-off culture base is 3-4g/L, preferably 3g/L.
Concentration of the agar powder in the Rooting and hardening-off culture base is 6-8g/L, preferably 7.5g/L.
It is preferred that above-mentioned Rooting and hardening-off culture base, be added in 1/2MS basal mediums paclobutrazol, sucrose and
Phytagel plant gels and be made；Wherein, the paclobutrazol, sucrose and Phytagel plant gels are trained in the strong plantlets and rootage
The concentration supported in base is respectively 0.5-1mg/L, 20g/L, 3g/L；Described its pH value of Rooting and hardening-off culture base is 5.7-6.0.
The invention provides above-mentioned plant establishment strong seedling culture base to cultivate plant test tube seedling, tissue cultures or genetic transformation
In application, the plant be rice, corn, wheat, barley, millet, sorghum, peanut, soybean, sweet potato, potato, cotton or
Rape.
In above-mentioned application, condition of culture is 28-35 DEG C of temperature, illumination cultivation, cultivation cycle 7-15d.
The invention provides application of the paclobutrazol in plant establishment strong seedling culture base is prepared, added in root media
Paclobutrazol, the final concentration of 0.3-2.5mg/L of paclobutrazol, the plant establishment strong seedling culture base can make plant seedlings take root
It hurry up, root is thicker, and root hair increases elongated, and transplanting survival rate is high, and after transplanting, seedling turns green soon, and growing way is good.
The root media includes but is not limited to 1/2MS culture mediums, 1/2N6 culture mediums or 1/2B5 culture mediums.
The present invention also provides above-mentioned Rooting and hardening-off culture base answering in plant test tube seedling, tissue cultures and genetic transformation
With.
The present invention also provides a kind of method for cultivating strong plantlets and rootage, comprises the following steps：The children that will be obtained through tissue cultures
Seedling is placed on above-mentioned Rooting and hardening-off culture base ready in advance and cultivated；Preferably, above-mentioned condition of culture is 28-35 DEG C of temperature,
Illumination cultivation, cultivation cycle 7-15d.
The advantage of the invention is that：
1st, the present invention adds 0.3-2.5mg/L paclobutrazol in culture medium, test tube seedling is become short strong, dark green leaf color, moves
Seedling turns green soon after cultivation, and growing way is good, improves the transplanting survival rate of test tube seedling.
2nd, condition of culture of the present invention is 28-35 DEG C, illumination cultivation, induces rooting of vitro seedling under this condition, is taken root fast, root
Thick and root hair increases elongated.
The present invention further optimizes plant establishment strong seedling culture base by groping and putting into practice, and finally finds out a kind of efficient
Plant establishment strong seedling culture base.The root media is to crops such as rice, corn, wheat, barley, peanut, soybean, cottons
Test tube seedling, tissue cultures and gene functional research etc. have stronger practical value.Rooting and hardening-off culture base of the present invention
With making, plant rooting of vitro seedling is fast, and when ordinary culture medium is used for culture of rootage, growing new root needs 2d, and uses the present invention
Culture medium then only needs 1d, and new root is thick and root hair grows (see Fig. 2) more, is turned green after the short strong, dark green leaf color (see Fig. 3) of seedling and transplanting
Hurry up, transplant just turn green after 2d, the advantages of growing way is good, so as to significantly improve the transplanting survival rate of test tube seedling, survival rate could (see Fig. 5)
Reach 98%, the final efficiency for improving research gene function and tissue culture breeding.
Brief description of the drawings
Fig. 1 is that root long, bud length and radical purpose of the rice seedling in different culture media after Rooting and hardening-off culture 15d become
Change.The culture medium of addition paclobutrazol can suppress cauline leaf excessive growth.1/2MS, any hormone is not added in root media；MET0.5、
MET1, MET1.5, MET2, MET2.5, MET3, MET5 be respectively added in root media paclobutrazol 0.5mg/L, 1mg/L,
1.5mg/L、2mg/L、2.5mg/L、3mg/L、5mg/L。
Fig. 2 is young root microstructure of the rice seedling in different culture media after Rooting and hardening-off culture 15d.Seedling is being added
Young root is sturdy in the culture medium of paclobutrazol and root hair is extremely more and long.1/2MS, any hormone is not added in root media；
MET0.5, MET1, MET1.5, MET2, MET2.5, MET3, MET5 be respectively added in root media paclobutrazol 0.5mg/L,
1mg/L、1.5mg/L、2mg/L、2.5mg/L、3mg/L、5mg/L。
Fig. 3 is upgrowth situation of the rice seedling in different culture media after Rooting and hardening-off culture 15d.Add the training of paclobutrazol
Support seedling in base it is short it is strong, blade is generous, dark green leaf color.1/2MS, any hormone is not added in root media；MET0.5, take root
Paclobutrazol 0.5mg/L is added in culture medium；MET1, MET1.5, MET2, MET2.5, MET3, MET5 are respectively in root media
Add paclobutrazol 1mg/L, 1.5mg/L, 2mg/L, 2.5mg/L, 3mg/L, 5mg/L.
Fig. 4 is growth of the rice seedling in the culture medium 1/2MSIBA of the IBA containing 0.5mg/L after Rooting and hardening-off culture 15d
Situation.Seedling growth is similar to 1/2MS in addition IBA culture medium, and seedling is elongated, and young root is short and thin.1/2MS, root media
In do not add any hormone；1/2MSIBA, 0.5mg/L IBA are added in root media.
Fig. 5 is that rice seedling after Rooting and hardening-off culture 15d, transplants 0d, 7d, 15d upgrowth situation in different culture media.
The seedling added when transplanting 7 days in paclobutrazol culture medium has begun to grow, but the seedling grown in 1/2MS culture mediums is still located
In period of seedling establishment.After transplanting 15 days, the Seedling Height in paclobutrazol culture medium is added more than in 1/2MS culture mediums.1/2MS,
Any hormone is not added in root media；MET0.5, paclobutrazol 0.5mg/L is added in root media；MET1, MET1.5,
MET2, MET2.5, MET3, MET5 be respectively added in root media paclobutrazol 1mg/L, 1.5mg/L, 2mg/L, 2.5mg/L,
3mg/L、5mg/L。
Embodiment
Following examples are used to illustrate the present invention, but are not limited to the scope of the present invention.Without departing substantially from spirit of the invention
In the case of essence, the modifications or substitutions made to the inventive method, step or condition belong to the scope of the present invention.
Unless otherwise specified, the conventional meanses that technological means used in embodiment is well known to those skilled in the art.
It is convenient to prepare, first prepare following mother liquor, then by mother liquor into each culture medium.Each mother liquor is by solvent and solute water
Composition, solute specific formula are as follows：
Embodiment 1
A kind of Rooting and hardening-off culture base (being abbreviated as MET0.25), it is to add paclobutrazol in 1/2MS basal mediums and make
；Wherein, concentration of the paclobutrazol in the Rooting and hardening-off culture base is 0.25mg/L；In the Rooting and hardening-off culture base also
Containing concentration be 20g/L sucrose, concentration be 3g/L Phytagel plant gels, pH value 5.7.
Embodiment 2
A kind of Rooting and hardening-off culture base (being abbreviated as MET0.5), it is to add paclobutrazol in 1/2MS basal mediums and make
；Wherein, concentration of the paclobutrazol in the Rooting and hardening-off culture base is 0.5mg/L；In the Rooting and hardening-off culture base also
Containing concentration be 20g/L sucrose, concentration be 3g/L Phytagel plant gels, pH value 5.8.
Embodiment 3
A kind of Rooting and hardening-off culture base (being abbreviated as MET0.75), it is to add paclobutrazol in 1/2MS basal mediums and make
；Wherein, concentration of the paclobutrazol in the Rooting and hardening-off culture base is 0.75mg/L；In the Rooting and hardening-off culture base also
Containing concentration be 20g/L sucrose, concentration be 3g/L Phytagel plant gels, pH value 6.0.
Embodiment 4
A kind of Rooting and hardening-off culture base (being abbreviated as MET1.5), it is to add paclobutrazol in 1/2MS basal mediums and make
；Wherein, concentration of the paclobutrazol in the Rooting and hardening-off culture base is 1.5mg/L；In the Rooting and hardening-off culture base also
Containing concentration be 20g/L sucrose, concentration be 3g/L Phytagel plant gels, pH value 5.7.
Embodiment 5
A kind of Rooting and hardening-off culture base (being abbreviated as MET1.75), it is to add paclobutrazol in 1/2MS basal mediums and make
；Wherein, concentration of the paclobutrazol in the Rooting and hardening-off culture base is 1.75mg/L；In the Rooting and hardening-off culture base also
Containing concentration be 20g/L sucrose, concentration be 3g/L Phytagel plant gels, pH value 5.9.
Embodiment 6
A kind of Rooting and hardening-off culture base (being abbreviated as MET2), it is to add paclobutrazol in 1/2MS basal mediums and be made；
Wherein, concentration of the paclobutrazol in the Rooting and hardening-off culture base is 2mg/L；Also containing dense in the Rooting and hardening-off culture base
Spend the sucrose for 20g/L, concentration is 3g/L Phytagel plant gels, pH value 5.7.
Embodiment 7
A kind of Rooting and hardening-off culture base (being abbreviated as MET2.25), it is to add paclobutrazol in 1/2MS basal mediums and make
；Wherein, concentration of the paclobutrazol in the Rooting and hardening-off culture base is 2.25mg/L；In the Rooting and hardening-off culture base also
Containing concentration be 20g/L sucrose, concentration be 3g/L Phytagel plant gels, pH value 6.0.
Embodiment 8
A kind of Rooting and hardening-off culture base (being abbreviated as MET2.5), it is to add paclobutrazol in 1/2MS basal mediums and make
；Wherein, concentration of the paclobutrazol in the Rooting and hardening-off culture base is 2.5mg/L；In the Rooting and hardening-off culture base also
Containing concentration be 20g/L sucrose, concentration be 3g/L Phytagel plant gels, pH value 5.8.
Embodiment 9
A kind of Rooting and hardening-off culture base (being abbreviated as MET2.75), it is to add paclobutrazol in 1/2MS basal mediums and make
；Wherein, concentration of the paclobutrazol in the Rooting and hardening-off culture base is 2.75mg/L；In the Rooting and hardening-off culture base also
Containing concentration be 20g/L sucrose, concentration be 3g/L Phytagel plant gels, pH value 5.8.
Embodiment 10
A kind of Rooting and hardening-off culture base (being abbreviated as MET3), it is to add paclobutrazol in 1/2MS basal mediums and be made；
Wherein, concentration of the paclobutrazol in the Rooting and hardening-off culture base is 3mg/L；Also containing dense in the Rooting and hardening-off culture base
Spend the sucrose for 20g/L, concentration is 3g/L Phytagel plant gels, pH value 5.9.
Embodiment 11
A kind of Rooting and hardening-off culture base (being abbreviated as MET3.25), it is to add paclobutrazol in 1/2MS basal mediums and make
；Wherein, concentration of the paclobutrazol in the Rooting and hardening-off culture base is 3.25mg/L；In the Rooting and hardening-off culture base also
Containing concentration be 20g/L sucrose, concentration be 3g/L Phytagel plant gels, pH value 5.8.
Embodiment 12
A kind of Rooting and hardening-off culture base (being abbreviated as MET5), it is to add paclobutrazol in 1/2MS basal mediums and be made；
Wherein, concentration of the paclobutrazol in the Rooting and hardening-off culture base is 5mg/L；Also it is containing concentration in the Rooting and hardening-off culture base
20g/L sucrose, concentration be 3g/L Phytagel plant gels, pH value 5.9.
Rooting and hardening-off culture base described in embodiment 1-12, its preparation method include each component is added into 1/2MS by formula
Produced in basal medium.
Embodiment 13
A kind of Rooting and hardening-off culture base, it is to add paclobutrazol in 1/2MS basal mediums and be made；Wherein, paclobutrazol
Concentration in the Rooting and hardening-off culture base is 1mg/L；Also containing the sugarcane that concentration is 20g/L in the Rooting and hardening-off culture base
Sugar, concentration be 3g/L Phytagel plant gels, pH value 5.8.
Embodiment 14
The present embodiment provides a kind of preparation method of Rooting and hardening-off culture base, and it is formulated with embodiment 13.Should to configure 1L
Exemplified by Rooting and hardening-off culture base, its preparation method includes：Take a large amount of (10 ×) 100ml, 1/2MS micro (100 ×) of 1/2MS
10ml, organic (100 ×) 10ml, add 20g sucrose after molysite (100 ×) 10ml mixing, addition paclobutrazol is complete to required concentration
PH 5.7-6.0 are adjusted after fully dissolved, obtain mixed liquor.Weigh 3g Phytagel plant gels and be dissolved in 800ml deionized waters, be placed in micro-
Boiled in ripple stove to being completely dissolved, add constant volume in above-mentioned mixed liquor and, to 1L, dispense into pipe of taking root, 116 DEG C of sterilizing 15min.
Comparative example 1 does not add paclobutrazol
A kind of culture medium 1/2MS, exemplified by configuring the 1L Rooting and hardening-off culture bases, its preparation method includes：Take 1/2MS big
Added after measuring (10 ×) 100ml, 1/2MS micro (100 ×) 10ml, organic (100 ×) 10ml, molysite (100 ×) 10ml mixing
20g sucrose, pH 5.7-6.0 are adjusted after being completely dissolved, obtain mixed liquor.Weigh 3g Phytagel plant gels be dissolved in 800ml go from
Sub- water, it is placed in micro-wave oven and boils to being completely dissolved, adds constant volume in above-mentioned mixed liquor and, to 1L, dispense into pipe of taking root, 116 DEG C
Sterilize 15min.
Comparative example 2 does not add paclobutrazol, adds IBA
A kind of culture medium 1/2MSIBA, exemplified by configuring the 1L Rooting and hardening-off culture bases, its preparation method includes：Take 1/
After 2MS a large amount of (10 ×) 100ml, 1/2MS micro (100 ×) 10ml, organic (100 ×) 10ml, molysite (100 ×) 10ml mixing
Add 20g sucrose, 0.5mg/L IBA；PH 5.7-6.0 are adjusted after being completely dissolved, obtain mixed liquor.Weigh 3g Phytagel plants
Gel is dissolved in 800ml deionized waters, is placed in micro-wave oven and boils to being completely dissolved, and adds constant volume in above-mentioned mixed liquor and, to 1L, divides
It is filled in pipe of taking root, 116 DEG C of sterilizing 15min.
Experimental example 1
Rice is chosen through the seedling that tissue cultures obtain as donor, be respectively placed in embodiment 2,4,6,8,10,12,13 and
Root induction, strong sprout are cultivated made from comparative example 1-2 on culture medium, condition of culture control is 28-35 DEG C of temperature, illumination cultivation,
Routine observation.Counted after culture 15d：After bud length, root long, radical, transplanting plant height and transplanting after survival rate.
Embodiment 13 Rooting and hardening-off culture base (being abbreviated as MET1) inducing paddy rice rooting of vitro seedling strong sprout result such as Fig. 1 institutes
Show：Test tube seedling is after paclobutrazol is handled, and compared with the test tube seedling of 1/2MS medium cultures, spire is short thick, dark green leaf color, healthy and strong,
Fast, radical of taking root increases, and root is sturdy and root hair increases elongated, as a result sees Fig. 1,2,3.Therefore, it is resistant to lodging after test tube transplantation of seedlings
Ability is strong, and survival rate is high, and survival rate can reach 98%, turn green soon, i.e. occurs as soon as and turn green after transplanting 2d, growth is fast, as a result sees figure
5。
Culture medium embodiment induction result made from the culture medium 1/2MS of comparative example 1 and embodiment 2,4,6,8,10,12,13
As shown in Fig. 1,2,3：Test tube seedling cultivates growth comparatively fast on the culture medium 1/2MS of comparative example 1, and spire growth is fast but elongated thin and weak,
Young root is thin and delicate, easily causes spire excessive growth, and survival rate is relatively low after transplanting, only up to reach 80%, and period of seedling establishment is grown, about 7-10
My god, as shown in Figure 5.
The culture medium 1/2MS of the comparative example 1 and culture medium 1/2MSIBA of comparative example 2 induction rooting of vitro seedling strong sprout results such as Fig. 4
It is shown：Test tube seedling root induction on these culture mediums, spire growth is fast elongated thin and weak, though root is more but thin and delicate and short, easily
Spire excessive growth is caused, survival rate is relatively low after transplanting, only up to reach 80%, and period of seedling establishment is grown, about 7-10 days, as shown in Figure 5.
Transplanting result after embodiment 13 Rooting and hardening-off culture base (being abbreviated as MET1) inducing paddy rice rooting of vitro seedling strong sprout
As shown in Figure 5：The test tube seedling handled through MET is turned green soon, and transplanting 7d rear blades are greener than control 1/2MS, there is obvious tiller, and long
Gesture is good, and plant height exceedes control, as shown in Figure 5.
Plant height is measured after 15d, experimental result is shown in Table 1：Although the test tube seedling handled through paclobutrazol before transplanting it is obvious it is short in
1/2MS is compareed, but after transplanting, the test tube seedling after paclobutrazol is handled adapts to rapidly environment, and 2 days start to turn green, and embody birth
Long advantage, its plant height exceed control gradually, as shown in Figure 5.And the trend embodies difference and become apparent from high temperature, when temperature is 26
When degree and the above, through the test tube seedling growing way that paclobutrazol is handled significantly better than control；When outdoor temperature be 22 degree and below when transplant
Test tube seedling, control and the test tube seedling growing way difference handled through paclobutrazol is not notable, as shown in table 1.Therefore, training provided by the invention
Support base to be applied to the tissue-cultured seedling strong plantlets and rootages of plant such as rice and subsequently transplant, the transplanting under the hot environment that is particularly suitable for use in.
The rice seedling of table 1 after Rooting and hardening-off culture 15d, transplants 15d plant height in different culture media
Several culture medium strong plantlets and rootage Contrast on effect of summary are it can be found that the culture medium test tube seedling that uses of the present invention is short
Strong, root is slightly and root hair increases elongated, dark green leaf color, generous, is turned green after transplanting fast, and test tube seedling leaf color is greener than compareing after transplanting 7d,
There is obvious tiller, and growing way is good.
To sum up, Rooting and hardening-off culture base provided by the invention makes plant by rationally adding the paclobutrazols (MET) of various concentrations
Thing seedling and contrast ratio seedling become short strong, and its dark green leaf color is generous, and the fast, root of taking root is thick and root hair is more and grows, after transplanting, children
Seedling is turned green soon, and growing way is good, final to improve research gene function and tissue culture breeding so as to improve the transplanting survival rate of plant test tube seedling
Efficiency.
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (3)
1. a kind of application of rice Rooting and hardening-off culture base in rice test tube seedling, tissue cultures or genetic transformation is cultivated, described
The condition of culture of culture medium be 28-35 DEG C of temperature, illumination cultivation, cultivation cycle 7-15d；
Wherein, described rice Rooting and hardening-off culture base is 1/2MS culture mediums, is by being added in 1/2MS basal mediums
Paclobutrazol, sugar and coagulator and be made；
Wherein, concentration of the paclobutrazol in the rice Rooting and hardening-off culture base is 0.5-1.5mg/L；Sucrose concentration is
15-25g/L；The Medium's PH Value is 5.7-6.0.
2. application as claimed in claim 1, it is characterised in that Phytagel plant gels that the coagulator is 3-4g/L or
6-8g/L agar powder；The Phytagel plant gels are the substitutes from a kind of agar of pseudomonad secretion.
3. the purposes of the rice Rooting and hardening-off culture base prepared by final concentration of 0.5-1.5mg/L paclobutrazol, the culture medium
Suitable for rice；
The purposes is rice seedling is taken root soon, and root is thicker, and root hair increases elongated, and transplanting survival rate is high, and after transplanting, seedling returns
Blue or green fast, growing way is good；
Characterized in that, paclobutrazol is added in Rooting and hardening-off culture base, the final concentration of 0.5-1.5mg/L of paclobutrazol；
Wherein, described rice Rooting and hardening-off culture base is 1/2MS culture mediums, is by being added in 1/2MS basal mediums
Paclobutrazol, sugar and coagulator and be made；
Wherein, concentration of the paclobutrazol in the Rooting and hardening-off culture base is 0.5-1.5mg/L；Sucrose concentration is 15-
25g/L；The coagulator is 3-4g/L Phytagel plant gels；The Medium's PH Value is 5.7-6.0.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610871415.5A CN106538381B (en) | 2016-09-30 | 2016-09-30 | A kind of plant establishment strong seedling culture base and preparation method and application containing paclobutrazol |
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