CN1203754C - Breeding technology of konjak testtube taro - Google Patents

Breeding technology of konjak testtube taro Download PDF

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Publication number
CN1203754C
CN1203754C CN 03135572 CN03135572A CN1203754C CN 1203754 C CN1203754 C CN 1203754C CN 03135572 CN03135572 CN 03135572 CN 03135572 A CN03135572 A CN 03135572A CN 1203754 C CN1203754 C CN 1203754C
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China
Prior art keywords
culture
bottom set
taro
temperature
konjaks
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Expired - Fee Related
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CN 03135572
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Chinese (zh)
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CN1493186A (en
Inventor
王玲
马继琼
房亚南
李勇军
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Biotechnology and Germplasm Resource Institute of Yunnan Academy of Agricultural Sciences
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Biotechnology and Germplasm Resource Institute of Yunnan Academy of Agricultural Sciences
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Priority to CN 03135572 priority Critical patent/CN1203754C/en
Publication of CN1493186A publication Critical patent/CN1493186A/en
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Publication of CN1203754C publication Critical patent/CN1203754C/en
Expired - Fee Related legal-status Critical Current
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Abstract

The present invention relates to test-tube konjak propagating technology which belongs to the technical field of agricultural biology. The present invention is composed of common propagation procedures, and also comprises: 1 to 6 ppm of paclobutrazol is added to rootage culture media with MS+NAA5-1 mg/L during the rootage culture of tissue culture seedlings of konjaks; the tissue culture seedlings of konjaks are cultured for 20 to 25 days in an environment with 1000 LX illumination and a temperature of more than 20 DEG C; when the tissue culture seedlings are rooted, tubers expands to form small corms; when the small corms grow to 2 to 4 grams, dark culture is carried out, and a culture temperature is below 15 DEG C; after the tissue culture seelings are cultured in bottles for 10 to 15 days, mature small corms are formed; the mature small corms are taken out of bottles, and are implanted into seedbeds after sprouting treatment; nutrients are exerted on the mature small corms under the condition of appropriate shielding at a temperature of more than 20 DEG C, and the exerted nutrients have the proportion of N to P to K to Ca to Mg as 5 to 1 to 5 to 0.5 to 1.100 to 200 grams of seed konjaks and four to five small lateral konjaks can be harvested by the propagating technology. The present invention has the advantages of high survival rate and reproduction rate of seed konjaks, simple management, etc.

Description

The raising technology of konjaku test-tube taro
Technical field:
The present invention relates to a kind of raising technology of konjaku test-tube taro, belong to agricultural biological technical field.
Background technology:
Konjaku is the unique plant that can synthesize Glucomannan in a large number, and Glucomannan all has extensive effect on industry, agricultural and medicine, health food, more and more cause people's interest and attention, and cultivated area constantly enlarges.But rate height, good strains of seeds serious degradation have restricted the development of this industry in spite of illness to plant taro.Breed virus-free high quality seedling, plant taro and on various plants such as potato, lily, obtained success by biotechnology, and extensively apply to produce, obtained the economic benefit of good social benefit.Konjaku can have been bred out good seed and be transplanted to the seedbed at present and carried out stock breeding by biotechnology, and effective solution is provided for konjaku kind sexual involution, good species taro are in great shortage.But the cultivation management of konjaku tissue cultivating seedling is a very meticulous job, and technology operation and cultivation condition do not note reducing greatly the survival rate and the knot taro rate of tissue cultivating seedling slightly.
Up to now, do not see the open report of breeding the konjaku bottom set in vitro.
Summary of the invention:
Management is difficult, specification requirement is high overcoming when the konjaku tissue cultivating seedling is transplanted for purpose of the present invention, is difficult for the deficiency of being held by support, and a kind of raising technology of managing the konjaku test-tube taro simple, that survival rate is high is provided.
The raising technology of konjaku test-tube taro of the present invention, by the flushing of konjaku explant, sterilization, inoculation, successive transfer culture, culture of rootage, induce bottom set and ripe bottom set bottle outlet to implant the seedbed, breeding stem tuber program is formed, wherein:
The paclobutrazol that adds 1-6ppm during konjaku tissue cultivating seedling culture of rootage in root media MS+NAA5-1mg/L was cultivated 20-25 days in the environment of temperature more than 20 ℃, illumination 1000LX, and stem tuber expanded when tissue cultivating seedling was taken root, and formed bottom set.When bottom set grows to 2-4 when gram, secretly to cultivate, cultivation temperature is below 15 ℃, and tissue cultivating seedling fell seedling in 10-15 days in bottle, form ripe bottom set.Ripe bottom set bottle outlet is implanted the seedbed after vernalization is handled, and is that one-period applies nutrition with 30 days under the condition that temperature is suitably sheltered from heat or light more than 20 ℃, and the ratio that applies nutrition is N: P: K: Ca: Mg=5: 1: 5: 0.5: 1.This numerous the region between the heart and the diaphragm technology just can be gathered in the crops kind taro and 4-5 boy taro of 100-200 gram then.
The flushing of above-mentioned konjaku explant, sterilization, inoculation, successive transfer culture and corresponding condition of culture are same as the prior art.
The present invention breeds bottom set in vitro by biotechnology, has reduced the program that test-tube seedling transplanting is managed this high request, has advantages such as the survival rate of kind of taro and reproduction rate height, management be simple.
Embodiment:
Earlier with tuberous konjaku explant flushing, and be that specification is got the terminal bud part by 0.5cm * 0.5cm, soaked 15 minutes with 75% alcohol-pickled 2~3 seconds and 0.1% mercuric chloride respectively again, use aseptic water washing immediately, konjaku stem tuber explant after the flushing is put into MS+6BA6-0.1mg/L+NAA6-0mg/L, PH5.8 medium, in temperature is more than 20 ℃, cultivates in the environment of illumination 1000LX and got final product once-seedling forming in 45 days.Put into tissue cultivating seedling same medium again and carry out successive transfer culture this moment, and every 20 days subcultures once, the propagation multiple is 1: 4~1: 6.Temperature in the successive transfer culture process is more than 20 ℃, illumination 1000LX.When seedling grows to 3~4cm when high, carry out the plant division cutting, put into root media MS+NAA5-1mg/L culture of rootage 20-25 days of the paclobutrazol that is added with 1-6ppm, stem tuber expanded when tissue cultivating seedling was taken root, and formed bottom set.When bottom set grows to 2-4 when gram, secretly to cultivate, cultivation temperature is below 15 ℃, and tissue cultivating seedling fell seedling in 10-15 days in bottle, form ripe bottom set.Ripe bottom set bottle outlet is implanted the seedbed after conventional vernalization is handled, and is that one-period applies nutrition with 30 days under the condition that temperature is suitably sheltered from heat or light more than 20 ℃, and the ratio that applies nutrition is N: P: K: Ca: Mg=5: 1: 5: 0.5: 1.This raising technology just can be gathered in the crops kind taro and 4-5 boy taro of 100-200 gram then.

Claims (1)

1, a kind of mating system of konjaku test-tube taro, by the flushing of konjaku explant, sterilization, inoculation, successive transfer culture, culture of rootage, induce bottom set and ripe bottom set bottle outlet to implant the seedbed, breeding stem tuber program is formed, it is characterized in that:
1.1 in root media MS+NAA5-1mg/L, add the paclobutrazol of 1-6ppm during konjaku tissue cultivating seedling culture of rootage, in the environment of temperature more than 20 ℃, illumination 1000LX, cultivated 20-25 days, stem tuber expanded when tissue cultivating seedling was taken root, and formed bottom set;
1.2 when bottom set grows to 2-4 when gram, secretly cultivate, cultivation temperature is below 15 ℃, tissue cultivating seedling fell seedling in 10-15 days in bottle, form ripe bottom set;
1.3 ripe bottom set bottle outlet is implanted the seedbed after vernalization is handled, and is that one-period applies nutrition with 30 days under the condition that temperature is sheltered from heat or light more than 20 ℃, the ratio that applies nutrition is N: P: K: Ca: Mg=5: 1: 5: 0.5: 1; This raising technology just can be gathered in the crops kind taro and 4-5 boy taro of 100-200 gram then.
CN 03135572 2003-08-09 2003-08-09 Breeding technology of konjak testtube taro Expired - Fee Related CN1203754C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 03135572 CN1203754C (en) 2003-08-09 2003-08-09 Breeding technology of konjak testtube taro

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 03135572 CN1203754C (en) 2003-08-09 2003-08-09 Breeding technology of konjak testtube taro

Publications (2)

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CN1493186A CN1493186A (en) 2004-05-05
CN1203754C true CN1203754C (en) 2005-06-01

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CN 03135572 Expired - Fee Related CN1203754C (en) 2003-08-09 2003-08-09 Breeding technology of konjak testtube taro

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Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100355333C (en) * 2004-09-17 2007-12-19 中国科学院昆明植物研究所 Bulbil konjak sprout breeding method
CN100469238C (en) * 2006-02-20 2009-03-18 云南师范大学 Method for producing test-tube carmus for high efficiency commercialized prodn. of devilstrongue
CN100370021C (en) * 2006-02-20 2008-02-20 云南师范大学 Capsular coating method for micro test tube corm of konjack
CN100574611C (en) * 2007-12-04 2009-12-30 云南省农业科学院生物技术与种质资源研究所 A kind of method for breeding konjak fine breed
CN102763597B (en) * 2012-08-15 2013-11-20 云南省农业科学院生物技术与种质资源研究所 Two-generation in one year reproduction method for konjac cross breeding
CN102986532A (en) * 2012-11-10 2013-03-27 上饶师范学院 Red-bud taro micro tuber callus induction, differentiation and plant regeneration method
CN103430822B (en) * 2013-09-09 2015-03-18 云南省农业科学院生物技术与种质资源研究所 Aquaculture seed reproduction method for micro seed tubers of konjac
CN105393788B (en) * 2015-12-17 2017-12-12 昆明瀚钰百林农业科技有限公司 A kind of cultural method of bulbil type konjac underground bulb high yield blade face bulb
CN113973717A (en) * 2021-11-29 2022-01-28 云南省热带作物科学研究所 Germination accelerating and seedling raising method for bulbil konjak tissue culture micro-corms

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