CN1493186A - Breeding technology of konjak testtube taro - Google Patents
Breeding technology of konjak testtube taro Download PDFInfo
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- CN1493186A CN1493186A CNA031355722A CN03135572A CN1493186A CN 1493186 A CN1493186 A CN 1493186A CN A031355722 A CNA031355722 A CN A031355722A CN 03135572 A CN03135572 A CN 03135572A CN 1493186 A CN1493186 A CN 1493186A
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- bottom set
- taro
- konjaku
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Abstract
A test tube technique for reproducing the konjak features that the paclobutrazol is added to the culture medium MS+NAA5-1 mg/L used for rooting the tissue culture seedlings of konjak, they are cultured at temp higher than 20 deg.C, under light radiation (1000 LX) for 20-25 days to form small bulb, when the weight of small bulb reeches 2-4 g they are cultured at lower than 15 deg.C in dark environment, after cultured in flask for 10-15 day they are sprouted and then transplanted in seedbed, and they are cultured at higher than 20 deg.C, while the fertilizer is proportionally and periodically applied.
Description
Technical field:
The present invention relates to a kind of numerous the region between the heart and the diaphragm technology of konjaku test-tube taro, belong to agricultural biological technical field.
Background technology:
Konjaku is the unique plant that can synthesize Glucomannan in a large number, and Glucomannan all has extensive effect on industry, agricultural and medicine, health food, more and more cause people's interest and attention, and cultivated area constantly enlarges.But rate height, good strains of seeds serious degradation have restricted the development of this industry in spite of illness to plant taro.Breed virus-free high quality seedling, plant taro and on various plants such as potato, lily, obtained success by biotechnology, and extensively apply to produce, obtained the economic benefit of good social benefit.Konjaku can have been bred out good seed and be transplanted to the seedbed at present and carried out stock breeding by biotechnology, and effective solution is provided for konjaku kind sexual involution, good species taro are in great shortage.But the cultivation management of konjaku tissue cultivating seedling is a very meticulous job, and technology operation and cultivation condition do not note reducing greatly the survival rate and the knot taro rate of tissue cultivating seedling slightly.
Up to now, do not see the open report of breeding the konjaku bottom set in vitro.
Summary of the invention:
Management is difficult, specification requirement is high overcoming when the konjaku tissue cultivating seedling is transplanted for purpose of the present invention, is difficult for the deficiency of being held by support, and a kind of numerous the region between the heart and the diaphragm technology of managing the konjaku test-tube taro simple, that survival rate is high is provided.
Numerous the region between the heart and the diaphragm technology of konjaku test-tube taro of the present invention, by the flushing of konjaku explant, sterilization, inoculation, successive transfer culture, culture of rootage, induce bottom set and ripe bottom set bottle outlet to implant the seedbed, breeding stem tuber program is formed, wherein:
During konjaku tissue cultivating seedling culture of rootage at root media MS+NAA
5～1The paclobutrazol that adds 1-6ppm among the mg/L was cultivated 20-25 days in the environment of temperature more than 20 ℃, illumination 1000LX, and stem tuber expanded when tissue cultivating seedling was taken root, and formed bottom set.When bottom set grows to 2-4 when gram, secretly to cultivate, cultivation temperature is below 15 ℃, and tissue cultivating seedling fell seedling in 10-15 days in bottle, form ripe bottom set.Ripe bottom set bottle outlet is implanted the seedbed after vernalization is handled, and is that one-period applies nutrition with 30 days under the condition that temperature is suitably sheltered from heat or light more than 20 ℃, and the ratio that applies nutrition is N: P: K: Ca: Mg=5: 1: 5: 0.5: 1.This numerous the region between the heart and the diaphragm technology just can be gathered in the crops kind taro and 4-5 boy taro of 100-200 gram then.
The flushing of above-mentioned konjaku explant, sterilization, inoculation, successive transfer culture and corresponding condition of culture are same as the prior art.
The present invention breeds bottom set in vitro by biotechnology, has reduced the program that test-tube seedling transplanting is managed this high request, has advantages such as the survival rate of kind of taro and reproduction rate height, management be simple.
Embodiment:
Earlier with tuberous konjaku explant flushing, and by 0.5cm * 0.5cm is that specification is got the terminal bud part, soaks 15 minutes with 75% alcohol-pickled 2～3 seconds and 0.1% mercuric chloride respectively again, uses aseptic water washing immediately, and the konjaku stem tuber explant after the flushing is put into MS+6BA
6～0.1Mg/L+NAA
6～0In mg/L, the PH5.8 medium, be more than 20 ℃, cultivate in the environment of illumination 1000LX and got final product once-seedling forming in 45 days in temperature.Put into tissue cultivating seedling same medium again and carry out successive transfer culture this moment, and every 20 days subcultures once, the propagation multiple is 1: 4～1: 6.Temperature in the successive transfer culture process is more than 20 ℃, illumination 1000LX.When seedling grows to 3～4cm when high, carry out the plant division cutting, put into the root media MS+NAA of the paclobutrazol that is added with 1-6ppm
5～1Among the mg/L culture of rootage 20-25 days, stem tuber expanded when tissue cultivating seedling was taken root, and formed bottom set.When bottom set grows to 2-4 when gram, secretly to cultivate, cultivation temperature is below 15 ℃, and tissue cultivating seedling fell seedling in 10-15 days in bottle, form ripe bottom set.Ripe bottom set bottle outlet is implanted the seedbed after conventional vernalization is handled, and is that one-period applies nutrition with 30 days under the condition that temperature is suitably sheltered from heat or light more than 20 ℃, and the ratio that applies nutrition is N: P: K: Ca: Mg=5: 1: 5: 0.5: 1.This numerous the region between the heart and the diaphragm technology just can be gathered in the crops kind taro and 4-5 boy taro of 100-200 gram then.
Claims (1)
1, a kind of numerous the region between the heart and the diaphragm technology of konjaku test-tube taro, by the flushing of konjaku explant, sterilization, inoculation, successive transfer culture, culture of rootage, induce bottom set and ripe bottom set bottle outlet to implant the seedbed, breeding stem tuber program is formed, it is characterized in that:
1.1 during konjaku tissue cultivating seedling culture of rootage at root media MS+NAA
5～1The paclobutrazol that adds 1-6ppm among the mg/L was cultivated 20-25 days in the environment of temperature more than 20 ℃, illumination 1000LX, and stem tuber expanded when tissue cultivating seedling was taken root, and formed bottom set;
1.2 when bottom set grows to 2-4 when gram, secretly cultivate, cultivation temperature is below 15 ℃, tissue cultivating seedling fell seedling in 10-15 days in bottle, form ripe bottom set;
1.3 ripe bottom set bottle outlet is implanted the seedbed after vernalization is handled, and is that one-period applies nutrition with 30 days under the condition that temperature is suitably sheltered from heat or light more than 20 ℃, the ratio that applies nutrition is N: P: K: Ca: Mg=5: 1: 5: 0.5: 1; This numerous the region between the heart and the diaphragm technology just can be gathered in the crops kind taro and 4-5 boy taro of 100-200 gram then.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03135572 CN1203754C (en) | 2003-08-09 | 2003-08-09 | Breeding technology of konjak testtube taro |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03135572 CN1203754C (en) | 2003-08-09 | 2003-08-09 | Breeding technology of konjak testtube taro |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1493186A true CN1493186A (en) | 2004-05-05 |
| CN1203754C CN1203754C (en) | 2005-06-01 |
Family
ID=34240031
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 03135572 Expired - Fee Related CN1203754C (en) | 2003-08-09 | 2003-08-09 | Breeding technology of konjak testtube taro |
Country Status (1)
| Country | Link |
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| CN (1) | CN1203754C (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100355333C (en) * | 2004-09-17 | 2007-12-19 | 中国科学院昆明植物研究所 | Bulbil konjak sprout breeding method |
| CN100370021C (en) * | 2006-02-20 | 2008-02-20 | 云南师范大学 | Capsular coating method for micro test tube corm of konjack |
| CN100469238C (en) * | 2006-02-20 | 2009-03-18 | 云南师范大学 | Method for producing test-tube carmus for high efficiency commercialized prodn. of devilstrongue |
| CN100574611C (en) * | 2007-12-04 | 2009-12-30 | 云南省农业科学院生物技术与种质资源研究所 | A kind of method for breeding konjak fine breed |
| CN102763597A (en) * | 2012-08-15 | 2012-11-07 | 云南省农业科学院生物技术与种质资源研究所 | Two-generation in one year reproduction method for konjac cross breeding |
| CN102986532A (en) * | 2012-11-10 | 2013-03-27 | 上饶师范学院 | Red-bud taro micro tuber callus induction, differentiation and plant regeneration method |
| CN103430822A (en) * | 2013-09-09 | 2013-12-11 | 云南省农业科学院生物技术与种质资源研究所 | Aquaculture seed reproduction method for micro seed tubers of konjac |
| CN105393788A (en) * | 2015-12-17 | 2016-03-16 | 昆明瀚钰百林农业科技有限公司 | Cultivation method for high yield of leaf surface bulbs of bulbel knojak underground bulbs |
| CN113973717A (en) * | 2021-11-29 | 2022-01-28 | 云南省热带作物科学研究所 | Germination accelerating and seedling raising method for bulbil konjak tissue culture micro-corms |
-
2003
- 2003-08-09 CN CN 03135572 patent/CN1203754C/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100355333C (en) * | 2004-09-17 | 2007-12-19 | 中国科学院昆明植物研究所 | Bulbil konjak sprout breeding method |
| CN100370021C (en) * | 2006-02-20 | 2008-02-20 | 云南师范大学 | Capsular coating method for micro test tube corm of konjack |
| CN100469238C (en) * | 2006-02-20 | 2009-03-18 | 云南师范大学 | Method for producing test-tube carmus for high efficiency commercialized prodn. of devilstrongue |
| CN100574611C (en) * | 2007-12-04 | 2009-12-30 | 云南省农业科学院生物技术与种质资源研究所 | A kind of method for breeding konjak fine breed |
| CN102763597A (en) * | 2012-08-15 | 2012-11-07 | 云南省农业科学院生物技术与种质资源研究所 | Two-generation in one year reproduction method for konjac cross breeding |
| CN102763597B (en) * | 2012-08-15 | 2013-11-20 | 云南省农业科学院生物技术与种质资源研究所 | Two-generation in one year reproduction method for konjac cross breeding |
| CN102986532A (en) * | 2012-11-10 | 2013-03-27 | 上饶师范学院 | Red-bud taro micro tuber callus induction, differentiation and plant regeneration method |
| CN103430822A (en) * | 2013-09-09 | 2013-12-11 | 云南省农业科学院生物技术与种质资源研究所 | Aquaculture seed reproduction method for micro seed tubers of konjac |
| CN105393788A (en) * | 2015-12-17 | 2016-03-16 | 昆明瀚钰百林农业科技有限公司 | Cultivation method for high yield of leaf surface bulbs of bulbel knojak underground bulbs |
| CN105393788B (en) * | 2015-12-17 | 2017-12-12 | 昆明瀚钰百林农业科技有限公司 | A kind of cultural method of bulbil type konjac underground bulb high yield blade face bulb |
| CN113973717A (en) * | 2021-11-29 | 2022-01-28 | 云南省热带作物科学研究所 | Germination accelerating and seedling raising method for bulbil konjak tissue culture micro-corms |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1203754C (en) | 2005-06-01 |
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Owner name: KUNMING INST OF BOTANY CAS Free format text: FORMER OWNER: INST. OF BIOLOGICAL TECHNOLOGY, YUNNAN PROV. ACADEMY OF AGRICULTURE Effective date: 20041231 |
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Effective date of registration: 20041231 Address after: 650223 East Road, Kunming, Yunnan Applicant after: Institute of Biotechnology and Germplasm Resources, Yunnan Academy of Agricultural Sciences Address before: 650223 East Road, Kunming, Yunnan Applicant before: Biological Technology Research Institute of Yunnan Agricultural Academy |
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