CN106717980A - The method that UV beta radiations improve mung bean shoot vegetable vitamin and carotene carotene content - Google Patents

The method that UV beta radiations improve mung bean shoot vegetable vitamin and carotene carotene content Download PDF

Info

Publication number
CN106717980A
CN106717980A CN201710018937.5A CN201710018937A CN106717980A CN 106717980 A CN106717980 A CN 106717980A CN 201710018937 A CN201710018937 A CN 201710018937A CN 106717980 A CN106717980 A CN 106717980A
Authority
CN
China
Prior art keywords
mung bean
bean sprout
carotene
vitamin
content
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201710018937.5A
Other languages
Chinese (zh)
Inventor
贵梦园
王丹
张亮
王瀚博
李景原
王太霞
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Henan Normal University
Original Assignee
Henan Normal University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Henan Normal University filed Critical Henan Normal University
Priority to CN201710018937.5A priority Critical patent/CN106717980A/en
Publication of CN106717980A publication Critical patent/CN106717980A/en
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
    • A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/14—Measures for saving energy, e.g. in green houses

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention discloses a kind of method that UV beta radiations improve mung bean shoot vegetable vitamin and carotene carotene content, belong to the production technical field of high-quality mung bean sprout vegetable.Technical scheme main points are：Take mung bean seed, cleaned up after soaking 4h under 30 DEG C of dark conditions with running water, the mung bean that will be soaked is moved into and germinateed in bean sprout growing machine, regulation indoor temperature is 20 DEG C and keeps constant, the growth time of mung bean sprout is calculated when immersion, mung bean sprout is taken out from bean sprout growing machine after 60h, and UV B irradiation is carried out under dark condition, by the UV B fluorescent tubes vertical hangings of 40W in mung bean sprout top radiation 1h.The present invention effectively increases the nutritional quality of mung bean sprout vegetable.

Description

The method that uv b radiation improves mung bean shoot vegetable vitamin and carotene carotene content
Technical field
The invention belongs to the production technical field of high-quality mung bean sprout vegetable, and in particular to a kind of uv b radiation improves green The method of beans shoot vegetable vitamin and carotene carotene content.
Background technology
Sprout vegetable refers to that, using crop seed or plant nutrition storage device official, being grown under dark or illumination condition can Edible sprout, tender shoots or young stem.Sprout vegetable have quality it is tender, rich in vitamin (Guo X.B., Li T., Tang K.X.&Liu R.H.(2012).Effect of germination on phytochemical profiles and antioxidant activity of mung bean sprouts(Vigna radiata).Journal of Agricultural and Food Chemistry, 60,11050-11055.) etc. food feature, while have being not take up agriculture Field, with short production cycle, working condition can artificial regulatory, can the production and processing feature such as factorial praluction.Therefore, sprout vegetable is received deeply Consumers in general and the favor of manufacturing enterprise.
Mung bean sprout vegetable is the common sprout produced by 3-4 days sprout growths using mung bean seed under dark condition Vegetables.Contain vitamin C, vitamin B in mung bean sprouts2With the nutriment such as carrotene.Mung bean sprouts has medical value very high, The traditional Chinese medical science thinks that mung bean sprouts is cool in nature sweet, can not only clearly hot summer weather, qualcomm meridian, solve all poison, moreover it is possible to kidney tonifying, nourishing and strengthening vital, reducing blood lipid and Softening blood vessel, anti-cancer, anti-aging.
Mung bean sprouts is produced under dark condition, therefore mung bean shoot vegetable has the advantages that cellulose is few, quality is tender.So And also just because of production under dark condition, although vitamin content can increase in mung bean germination process, but also much than not On the green vegetables that grow in the sun.One of plant growth and the most important factor of metabolism are just influenceed, numerous studies are proved, Light quality species and intensity of illumination have a significant impact to vegetable nutrient composition.(Hu Y.B., the Sun G.Y.＆Wang X.C. such as Hu (2007).Induction characteristics and response of photosynthetic quantum conversion to changes in irradiance in mulberry plants.Journal of Plant Physiology, 164,959-968.) point out that rational light intensity can promote photosynthesis and dry-matter accumulation, and excess Irradiation can cause plant light injury and cause obvious influence to growing for plant.(the Li Q.＆Kubota C. such as Li (2009).Effects of supplemental light quality on growth and phytochemicals of Baby leaf lettuce.Environmental and Experimental Botany, 67,59-64.) etc. find with it is white Light can lift anthocyanidin content in lettuce compared to uv b radiation and blue light, and far-red light can then reduce its anthocyanidin content. (Jiang Z., Zheng Y., Qiu R., Yang Y., Xu M., Ye Y.＆Xu M. (2015) .Short UV-B such as Jiang exposure stimulated enzymatic and nonenzymatic antioxidants and reduced oxidative stress of cold-stored mangoes.Journal of Agricultural and Food Chemistry, 63,10965-10972.) research have shown that UV-B irradiation can increase ascorbic acid content in mango.In a word, UV- Beta radiation is used to improve plant nutrient quality by increasing pass (Wargent J.J.＆Jordan as physics regulation technology B.R.(2013).From ozone depletion to agriculture:understanding the role of UV radiation in sustainable crop production.New Phytologist,197,1058-1076.)。
Therefore, this research finds that UV-B can improve vitamin C, vitamin B in mung bean sprout under irradiating by determining2With The content of carrotene, is improved mung bean sprout nutritional quality, and this method is also for the production of high-quality shoot vegetable provides new Method.
The content of the invention
Present invention solves the technical problem that there is provided a kind of uv b radiation improves mung bean shoot vegetable vitamin and carrotene The method of content, the method can be improved the nutritional quality of mung bean sprout vegetable.
The present invention adopts the following technical scheme that uv b radiation improves mung bean shoot vegetable vitamin to solve above-mentioned technical problem With the method for carotene carotene content, it is characterised in that detailed process is：Mung bean seed is taken, is cleaned up after 30 DEG C with running water 4h is soaked under dark condition, the mung bean that will be soaked is moved into and germinateed in DY801B type bean sprout growing machines, regulation indoor temperature is 20 DEG C and keep constant, since immersion when calculate mung bean sprout growth time, by mung bean sprout from DY801B types bean sprouts after 60h Taken out in machine, and UV-B irradiation is carried out under dark condition, the UV-B fluorescent tubes vertical hanging of 40W is radiated in mung bean sprout top 1h.
The present invention is most suitable with the UV-B fluorescent tubes radiation 1h that power is 40W in the production of mung bean shoot vegetable, now, vitamin B2Highest is reached with the content of carrotene, Vitamin C content applies the UV- of appropriateness close to highest in the production of mung bean shoot vegetable Beta radiation is to improve vitamin B in mung bean shoot vegetable2, carrotene and Vitamin C content effective ways.
Specific embodiment
The above of the invention is described in further details by the following examples, but this should not be interpreted as this The scope for inventing above-mentioned theme is only limitted to following embodiment, and all technologies realized based on the above of the present invention belong to this hair Bright scope.
Embodiment
1 materials and methods
1.1 experimental designs
Mung bean seed is taken, is cleaned up after 4h is soaked under 30 DEG C of dark conditions with running water, the mung bean that will be soaked is moved Enter and germinateed in DY801B type bean sprout growing machines, regulation indoor temperature is 20 DEG C and to keep constant, when immersion calculate mung bean The growth time of sprout, takes out mung bean sprout from DY801B type bean sprout growing machines after 60h, is divided into 5 groups, and enter under dark condition Row UV-B is irradiated.
In mung bean sprout top, measure its irradiation intensity with ultraviolet irradiation intensity meter is UV-B fluorescent tubes (40W) vertical hanging 20.5μW/cm2.Exposure time (intensity) is respectively 0h (0kJ/m2)、0.5h(0.369kJ/m2)、1h(0.738kJ/m2)、1.5h (1.107kJ/m2) and 2h (1.476kJ/m2), wherein 0h (0kJ/m2) group is as a control group.
The measure of Vitamin C content in 1.2 mung bean sprouts
Using ascorbic acid content in molybdenum blue colorimetric method measure mung bean sprout.The fresh mung bean sprouts of 0.5g accurately are weighed, plus Enter 4mL oxalic acid-EDTA solution lucifuges to be fully ground, homogenate is transferred in centrifuge tube, 10000g, 4 DEG C of centrifugation 15min, supernatant As extract solution.Accurate 2mL extract solutions of drawing are placed in 25mL volumetric flasks, sequentially add 0.5mL metaphosphoric acids-acetic acid solution (3% (W/V) 10% (V/V)), 1mL 5% ((V/V)) sulfuric acid solution, 2mL 5% ((W/V)) ammonium molybdate solution.Use distilled water constant volume To 25mL, mix, 30 DEG C of warm bath 15min, mensuration absorbance value A under 760nm.The ascorbic acid solution of 0.4g/L is used as measurement institute Use standard items.Vitamin C is represented (mg/100g FW) with per ascorbic acid contained by 100g unit sample fresh weights.Data are used flat Mean value ± SD is represented (n=3).
Vitamin B in 1.3 mung bean sprouts2The measure of content
(1) preparation of standard liquid
Take vitamin B2Standard liquid (10.0 μ g/mL) 1.00mL, 2.00mL, 3.00mL, 4.00mL and 5.00mL distinguishes It is placed in the volumetric flask of 50mL, it is each to add deionized water to be diluted to scale, shake up.Labeled as a series of vitamin Bs2Standard is molten Liquid, it is to be measured.
(2) prepared by prepare liquid
Take 5.00mL unknown solutions to be placed in 50mL volumetric flasks, add deionized water to be diluted to scale, shake up, it is to be measured.
(3) drafting of excitation spectrum and fluorescence emission spectrum
It is launch wavelength to set λ em=540nm, and the scanning standard solution in the range of 250-500nm, record fluorescent emission is strong The relation curve of degree and excitation wavelength, just obtains excitation spectrum.Its maximum excitation wavelength X ex is found out from exciting light spectrogram. Under this excitation wavelength, scanned in the range of 400-600nm, the functional relation between record emissive porwer and launch wavelength is just obtained Fluorescence emission spectrum, finds out its maximum emission wavelength λ em from fluorescence emission spectrum.
(4) fluoremetry of standard liquid and sample
Excitation wavelength is fixed on maximum excitation wavelength X ex, fluorescence emission wavelengths are fixed on λ at maximum emission wavelength em.The fluorescent emission intensity of scanning distilled water and above-mentioned series standard liquid.Fluorescent emission intensity with solution as ordinate, standard Solution concentration is abscissa, makes standard curve.
The fluorescence intensity of unknown solution is determined under similarity condition, and vitamin B in unknown sample is determined by standard curve2 Concentration, calculate testing sample solution in vitamin B2Content.
The measure of carotene carotene content in 1.4 mung bean sprouts
The measure of carrotene in mung bean sprout, uses《National Standard of the People's Republic of China》GB/T.5009.83- 2003, the measure of carrotene in food.
1.5 statistical analyses
Statistical analysis is carried out using SPSS14.0 (SPSS Inc., Chicago, IL, USA).Use one-way analysis of variance (ANOVA) analysis result, the difference between using Tukey ' s multiple comparative tests to detect average value is divided using Pearson is related Analysis is to carrying out correlation analysis between each variable.Significant difference is defined as p<0.05.All data are expressed as average value ± mark Quasi- deviation (n=3).
2 results and analysis
Vitamin C, vitamin B in the lower mung bean sprout of UV-B irradiation2Change such as following table with carotene carotene content.
UV-B exposure times (h) 0 0.5 1 1.5 2
Vitamin C (mg/100gFW) 20.20±0.50 21.36±0.16 28.83±0.23 29.16±0.12 29.20±0.26
Vitamin B2(mg/100gFW) 0.60±0.01 0.76±0.04 0.83±0.03 0.79±0.04 0.80±0.06
Carrotene (mg/100gFW) 1.66±0.08 2.19±0.06 2.37±0.03 2.21±0.05 2.19±0.09
Test result indicate that, the uv b radiation of appropriateness can stimulate mung bean sprout to synthesize more vitamin Cs, vitamin B2With Carrotene, so that vitamin C, vitamin B in mung bean shoot vegetable2Increase with carotene carotene content, improve mung bean shoot vegetable Nutrient composition content.Wherein, uv b radiation 1h, Vitamin C content quickly increases (increase by 42.72%), is further added by uv b radiation Time, Vitamin C content is increased slightly.Uv b radiation 1h, vitamin B2Dramatically increased with carotene carotene content, wherein vitamin B2 Content increases by 38.33%, and carotene carotene content increases by 42.77%, continues to extend the uv b radiation time, vitamin B2And carrotene Content is not further added by, and is declined slightly.
Embodiment above describes general principle of the invention, principal character and advantage, the technical staff of the industry should Understand, the present invention is not limited to the above embodiments, simply original of the invention is illustrated described in above-described embodiment and specification Reason, under the scope for not departing from the principle of the invention, various changes and modifications of the present invention are possible, and these changes and improvements each fall within In the scope of protection of the invention.

Claims (1)

  1. The method that 1.UV-B radiation improves mung bean shoot vegetable vitamin and carotene carotene content, it is characterised in that detailed process is：Take Mung bean seed, is cleaned up after 4h is soaked under 30 DEG C of dark conditions with running water, and the mung bean that will be soaked is moved into bean sprout growing machine Germinateed, regulation indoor temperature is 20 DEG C and to keep constant, when immersion calculate the growth time of mung bean sprout, 60h Mung bean sprout is taken out from bean sprout growing machine afterwards, and UV-B irradiation is carried out under dark condition, by the UV-B fluorescent tube vertical hangings of 40W In mung bean sprout top radiation 1h.
CN201710018937.5A 2017-01-11 2017-01-11 The method that UV beta radiations improve mung bean shoot vegetable vitamin and carotene carotene content Pending CN106717980A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710018937.5A CN106717980A (en) 2017-01-11 2017-01-11 The method that UV beta radiations improve mung bean shoot vegetable vitamin and carotene carotene content

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710018937.5A CN106717980A (en) 2017-01-11 2017-01-11 The method that UV beta radiations improve mung bean shoot vegetable vitamin and carotene carotene content

Publications (1)

Publication Number Publication Date
CN106717980A true CN106717980A (en) 2017-05-31

Family

ID=58949128

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710018937.5A Pending CN106717980A (en) 2017-01-11 2017-01-11 The method that UV beta radiations improve mung bean shoot vegetable vitamin and carotene carotene content

Country Status (1)

Country Link
CN (1) CN106717980A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108668557A (en) * 2018-04-16 2018-10-19 江苏省农业科学院 It is a kind of to utilize method of germination corn enrichment lutein and products thereof and application
WO2020032601A3 (en) * 2018-08-09 2020-05-07 서울바이오시스 주식회사 Plant cultivation apparatus, and cultivation method using same
CN111418380A (en) * 2020-04-15 2020-07-17 厦门大学 Illumination culture method for promoting green stalk vegetable and four-nine heart vegetable to increase green

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101731133A (en) * 2010-01-20 2010-06-16 南京财经大学 Method for producing mung bean sprout
CN103181528A (en) * 2013-04-07 2013-07-03 苏州众仕达蔬菜食品有限公司 Production technology for mung bean sprouts
CN103636479A (en) * 2013-12-06 2014-03-19 占行波 Method for cultivating mung bean sprouts by oneself
CN104642079A (en) * 2014-06-24 2015-05-27 何叶 Method of cultivating bean sprouts

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101731133A (en) * 2010-01-20 2010-06-16 南京财经大学 Method for producing mung bean sprout
CN103181528A (en) * 2013-04-07 2013-07-03 苏州众仕达蔬菜食品有限公司 Production technology for mung bean sprouts
CN103636479A (en) * 2013-12-06 2014-03-19 占行波 Method for cultivating mung bean sprouts by oneself
CN104642079A (en) * 2014-06-24 2015-05-27 何叶 Method of cultivating bean sprouts

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
司超: "紫外线B对采后药用菊花花序生理特性和品质的影响及其机制", 《中国优秀硕士学位论文全文数据库农业科技辑》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108668557A (en) * 2018-04-16 2018-10-19 江苏省农业科学院 It is a kind of to utilize method of germination corn enrichment lutein and products thereof and application
CN108668557B (en) * 2018-04-16 2021-01-19 江苏省农业科学院 Method for enriching lutein by using germinated corn, product and application thereof
WO2020032601A3 (en) * 2018-08-09 2020-05-07 서울바이오시스 주식회사 Plant cultivation apparatus, and cultivation method using same
CN111418380A (en) * 2020-04-15 2020-07-17 厦门大学 Illumination culture method for promoting green stalk vegetable and four-nine heart vegetable to increase green

Similar Documents

Publication Publication Date Title
CN100546474C (en) A kind of method that promotes fruit color
CN109197219A (en) A kind of method for growing vegetables and LED light source
CN105580735B (en) Culture solution capable of improving resveratrol content in polygonum cuspidatum callus and culture method
CN106717980A (en) The method that UV beta radiations improve mung bean shoot vegetable vitamin and carotene carotene content
CN104351665A (en) Preparation method of high-flavonoid-content tartary buckwheat product
CN112273085B (en) Method for improving lettuce quality based on FR and UVA
CN104974914A (en) Blueberry wine formula
Linatoc et al. Influence of light intensity on the photosynthesis and phenolic contents of Mangifera indica
CN105379571B (en) A kind of method of anthocyanin accumulation in promotion Roselle calyx
Osuna-Ruíz et al. Enhancement of in-vitro antioxidant properties and growth of amaranth seed sprouts treated with seaweed extracts
KR101258275B1 (en) A method of cultivation soy bean sprout by using black garlic extract and the product thereby
Joshi et al. Optimization of extraction treatment and concentration of extract on yield and quality of anthocyanins from plum var.‘Santa Rosa’
CN104172002B (en) A kind of method preparing green Bulbus Allii
CN115777806A (en) Tea spreading/withering method
Fariña et al. Carotenoid profile evolution in Vitis vinifera cv. Tannat grapes during ripening
CN107496251A (en) A kind of Skin whitening care cosmeticses containing Chinese herbaceous peony stem cell extract
CN104286143B (en) A kind of ultraviolet irradiation preservation method of olive fruits
EP3369308B1 (en) Method for increasing content of coumestrol of germinated and fermented beans by using light emitting diodes
Deis et al. Effect of water stress in grape berries Cabernet Sauvignon (Mendoza, Argentina) during four years consecutives
Deloire New method to determine optimal ripeness for white wine styles
Jafari et al. Evaluation of far-infrared drying on the quality properties of zucchini slices: influence of operating parameters
Canton et al. Chlorophyll evaluation on leaves of'Sauvignon Blanc'during vegetative growth in São Joaquim, Santa Catarina, Brazil
Siriwattananon et al. Effect of ethylene curing on the physical properties and nutritional contents of Thai’s banana (Kluai Namwa)
CN101889511B (en) Processing technology for planting and dehydrating selenium-enriched leaf mustard
Angelov et al. Comparison of processing fruit quality of several grape varieties cultivated in climatic conditions of Poland and Bulgaria

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20170531