CN102726183A - Cultivation method for improving polysaccharide content of dendrobium candidum - Google Patents
Cultivation method for improving polysaccharide content of dendrobium candidum Download PDFInfo
- Publication number
- CN102726183A CN102726183A CN2012100966520A CN201210096652A CN102726183A CN 102726183 A CN102726183 A CN 102726183A CN 2012100966520 A CN2012100966520 A CN 2012100966520A CN 201210096652 A CN201210096652 A CN 201210096652A CN 102726183 A CN102726183 A CN 102726183A
- Authority
- CN
- China
- Prior art keywords
- dendrobium candidum
- light
- led lamp
- content
- film
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention discloses a cultivation method for improving polysaccharide content of dendrobium candidum. The dendrobium candidum is cultivated in red light or in mixed light of the red light and blue light. The cultivation method disclosed by the invention can effectively improve the polysaccharide content of the dendrobium candidum; the polysaccharide content of the dendrobium candidum cultivated by the method meets the specification of a pharmacopeia; and the content of alkaloid is not reduced. Furthermore, according to the cultivation method, the energy consumption is low, the increased cost is less, and the polysaccharide content of the dendrobium candidum can be greatly improved.
Description
Technical field
The invention belongs to the plant cultivation field, be specifically related to a kind of cultivation method that improves Dendrobium officinale polysaccharide content.
Background technology
Dendrobium candidum (Dendrobium officinale Kimura et Migo) is China's tradition rare traditional Chinese medicine; Have reinforcing stomach reg fluid, unique effects such as nourishing Yin and clearing heat; Be used to treat diseases such as atrophic gastritis, hypertension, diabetes, tumour, and the anti-ageing effect of waiting for a long time is arranged.
Polysaccharide is the main component of dendrobium candidum, with pharmacological action close getting in touch is arranged, and the height of polysaccharide mass fraction is a main foundation of judging the dendrobium candidum quality at present, and " Chinese pharmacopoeia is classified polysaccharide as dendrobium candidum " assay " index to version in 2010.But; Owing to reasons such as kind, cultivations; Some dendrobium candidum medicinal materials can not circulate on market because of the requirement that its polyoses content does not reach the pharmacopeia regulation, and therefore, the polyoses content that how to improve the dendrobium candidum medicinal material is a problem demanding prompt solution in the dendrobium candidum industry development.
Summary of the invention
In order to address the above problem, the present invention provides a kind of cultivation method that improves Dendrobium officinale polysaccharide content.
The cultivation method of raising Dendrobium officinale polysaccharide content provided by the invention, it is for to cultivate dendrobium candidum under ruddiness or ruddiness and blue light mixed light condition.
In one embodiment of the invention, be light source with the LED lamp of redness, dendrobium candidum is cultivated.
In another embodiment of the invention, cover dendrobium candidum with red film and cultivate.
In yet another embodiment of the present invention, the LED lamp with redness is a light source cultivation dendrobium candidum with blue LED lamp.
Wherein, can natural daylight arranged or not have under the natural daylight condition, be light source cultivation dendrobium candidum with required LED lamp.
In another embodiment, cover dendrobium candidum with red film, and the LED lamp with blueness is a light source cultivation dendrobium candidum in film; Or cover dendrobium candidum with blue membrane, and the LED lamp with redness is a light source cultivation dendrobium candidum in film.
Among the present invention, the spectral wavelength of said ruddiness is 620～760nm, and the spectral wavelength of said blue light is 455～492nm.
Cultivation method of the present invention can improve the polyoses content in the dendrobium candidum effectively, and presses the dendrobium candidum of the inventive method cultivation, and its polyoses content all satisfies the regulation of pharmacopeia, and alkaloids content does not reduce yet.In addition, the present invention is consumed energy low, and cost increases few, but can improve the polyoses content in the dendrobium candidum greatly.
Embodiment
Following examples are used to explain the present invention, but are not used for limiting scope of the present invention.
Embodiment 1
Choose the dendrobium candidum of four provenances, seen table 1.
Carry out Artificial Control pollination to the dendrobium candidum of each provenance in June, 2009, carried out aseptic seeding during fruit maturation on November 20th, 2009.
Table 1 dendrobium candidum provenance
The medium of seed germination is 1/2MS+6-benzyladenine (BA)+indolebutyric acid (IBA)+20% banana extract+2% active carbon+sucrose; Protocorm and strong seedling cultivation medium are 1/2MS+ indolebutyric acid (IBA)+20% banana extract+2% active carbon+sucrose.
Select in the vial that highly about 1.5cm seedling is inoculated in (diameter 7cm, high 10cm) respectively every bottle 8 strain.With different LED is that light source is cultivated, and regulates the distance of LED lamp control software and light source and plant, makes light quantity all at 50 μ molm
-2S
-1About, the photoperiod is 16hd
-1, cultivate 90d.Group training chamber relative moisture 70% ± 5%, temperature (24 ± 1) ℃.
Wherein, light source is the LED lamp available from Chinese emblem, Hangzhou Electro-optical Technology, INC. (US) 62 Martin Road, Concord, Massachusetts 017.Concrete technical parameter is seen table 2.
The important technological parameters of table 2 different light medium led light source
After three months, after seedling is cultivated and finished, add up the sprout tillers number of every bottle of seedling, ruler is measured the long and height of seedling of root, and it is thick that slide measure is measured stem.Reference literature (Wang Xuekui, 2006) is measured chlorophyll content, and acid-dye colorimetry is measured total alkaloid content (Zhu Yan etc., 2010), the content of phenolsulfuric acid colorimetric method for determining polysaccharide (Zhu Yan etc., 2010).With every bottle of measured value is the unit, and with Excel 2003 disposal datas, DPS software carries out variance analysis.
The statistics of different light medium seedlings of Dendrobium officinale economical character, chlorophyll and active ingredient is listed in table 3.
Table 3 light quality is to the influence of seedlings of Dendrobium officinale economical character, chlorophyll and active ingredient
Annotate: different 5% significance level that reaches of different lowercase alphabet differentials, down together.
Visible simultaneously by table 3, all there is appreciable impact in the LED light quality to seedlings of Dendrobium officinale chlorophyll and active constituent content.The luffing of chlorophyll content is: 1.058mgg
-1FM～1.574mgg
-1FM, mixed light 1 is handled the highest, is 1.574mgg
-1FM is than the high 0.187mgg of CK
-1FM; G handles minimum, is 1.058mgg
-1FM is than the low 0.329mgg of CK
-1FM; During all are handled; The chlorophyll content of ruddiness and 3 mixed lights is higher than CK; And blue light, gold-tinted and green glow significantly are lower than CK, explain that monochromatic ruddiness and red blue mixed light more help chlorophyllous accumulation, and it is chlorophyllous synthetic that blue light, gold-tinted and green glow then suppress in various degree.
Can find out that from table 3 polyoses content of ruddiness and 3 mixed lights all is significantly higher than CK.Mixed light 1 is handled the highest, is 8.90%, and is high by 2.79% than CK; It is minimum that green glow is handled, and only 3.74%, than CK low 2.37%.The alkaloid luffing is: 0.021%～0.042%, and blue light is handled the highest, is 0.042%, and is high by 0.01% than CK; Green glow is handled minimum, is 0.021%, than CK low 0.011%.Above result shows that red blue mixed light and ruddiness help the accumulation of polysaccharide, and blue light then can promote alkaloidal synthetic, and green glow suppresses polysaccharide and the accumulation of alkaloid in plant to some extent.
Embodiment 2
The dendrobium candidum axenic seedlings of four provenances among the embodiment 1 is carried out the greenhouse transplanting by conventional method.The dendrobium candidum of each provenance carries out following four groups of tests.100 test-tube plantlets of every group of test, triplicate.
Group 1: cultivate under the red film; The ruddiness light quantity of measuring that sees through film is 50 μ molm
-2S
-1
Group 2: cultivate under the red film, and be light source with the LED lamp of blueness; The ruddiness light of measuring in the film that sees through film is 40 μ molm
-2S
-1, the light quantity that the blue led lamp sends is 10 μ molm
-2S
-1
Group 3: cultivate under the blue membrane, and be light source with the LED lamp of redness; The blue light quantity of measuring in the film that sees through film is 20 μ molm
-2S
-1, the light quantity that red LED lamp is sent is 30 μ molm
-2S
-1
Control group 4: cultivate under the colorless film.The light quantity of measuring in the film is 50 μ molm
-2S
-1
In the test, redness, blueness and colorless film reach plastic film factory by Wenzhou, Zhejiang hundred million and produce.Other cultivation conditions of each experimental group are identical, are the conventional cultivation method of this area.
After 12 months, measure Dendrobium officinale polysaccharide content, assay method is referring to 2010 editions Chinese Pharmacopoeia, and the result sees table 4.
The difference of Dendrobium officinale polysaccharide content under the different films of table 4
Can find out that from table 4 red light and ruddiness and blue light mixed light cultivation dendrobium candidum can improve the polyoses content of dendrobium candidum.Ruddiness light transmission capacity 40 μ molm wherein
-2S
-1, blue light 10 μ molm
-2S
-1More can improve Dendrobium officinale polysaccharide content significantly, the lower provenance of some Dendrobium officinale polysaccharide content particularly, the product that adopts method of the present invention also can make not reach originally pharmacopeia to require becomes and meets the product that pharmacopeia requires.
Embodiment 3
The dendrobium candidum axenic seedlings of four provenances among the embodiment 1 is carried out the booth transplanting by conventional method.The dendrobium candidum of each provenance carries out following four groups of tests.100 test-tube plantlets of every group of test, triplicate.
Group 1: set up the red-light LED lamp in the colourless film.
Group 2: set up red-light LED lamp and blue LED lamp in the colourless film, wherein red-light LED: blue-ray LED is 4: 1.
Group 3: set up red-light LED lamp and blue LED lamp in the colourless film, wherein red-light LED: blue-ray LED is 7: 3.
Organize in the 4 colourless films and set up red-light LED lamp and blue LED lamp, wherein red-light LED: blue-ray LED is 3: 2.
Control group 5: set up white light LEDs in the colourless film.
In 5 groups of tests, the power and the distribution density of the LED lamp of selecting for use are identical, and it is identical to control 5 groups of cultivation conditions in the test.
After 12 months, measure Dendrobium officinale polysaccharide content, assay method is referring to 2010 editions Chinese Pharmacopoeia, and the result sees table 5.
The difference of Dendrobium officinale polysaccharide content under the table 5LED lamp
From table 5, can find out, augment the polyoses content that ruddiness or ruddiness and blue light also can effectively improve dendrobium candidum.Red-light LED wherein: blue-ray LED is that 4: 1 effect is better obviously, can improve polyoses content greatly.
The above only is a preferred implementation of the present invention; Should be pointed out that for those skilled in the art, under the prerequisite that does not break away from know-why of the present invention; Can also make some improvement and retouching, these improvement and retouching also should be regarded as protection scope of the present invention.
Claims (5)
1. a cultivation method that improves Dendrobium officinale polysaccharide content is characterized in that, under ruddiness or ruddiness and blue light mixed light condition, cultivates dendrobium candidum.
2. method according to claim 1 is characterized in that, is light source with the LED lamp of redness, and dendrobium candidum is cultivated.
3. method according to claim 1 is characterized in that, covers dendrobium candidum with red film and cultivates.
4. method according to claim 1 is characterized in that, is light source cultivation dendrobium candidum with the LED lamp of redness and the LED lamp of blueness.
5. method according to claim 1 is characterized in that, covers dendrobium candidum with red film, and the LED lamp with blueness is a light source cultivation dendrobium candidum in film; Or cover dendrobium candidum with blue membrane, and the LED lamp with redness is a light source cultivation dendrobium candidum in film.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100966520A CN102726183A (en) | 2012-01-20 | 2012-04-05 | Cultivation method for improving polysaccharide content of dendrobium candidum |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210017875.3 | 2012-01-20 | ||
| CN201210017875 | 2012-01-20 | ||
| CN2012100966520A CN102726183A (en) | 2012-01-20 | 2012-04-05 | Cultivation method for improving polysaccharide content of dendrobium candidum |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102726183A true CN102726183A (en) | 2012-10-17 |
Family
ID=46983074
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012100966520A Pending CN102726183A (en) | 2012-01-20 | 2012-04-05 | Cultivation method for improving polysaccharide content of dendrobium candidum |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102726183A (en) |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103931429A (en) * | 2014-03-14 | 2014-07-23 | 云南云琦丹生物科技有限公司 | Artificial light seedling culture method capable of promoting plant growth and differentiation |
| CN104026016A (en) * | 2014-06-20 | 2014-09-10 | 山东理工大学 | Method for producing dendrobium polysaccharides by inducing dendrobium candidum by jasmonic acid methyl ester |
| CN104285632A (en) * | 2014-09-10 | 2015-01-21 | 韦俊 | Cultivation method for improving polysaccharide content of Chinese yam |
| CN104855262A (en) * | 2015-06-05 | 2015-08-26 | 杭州正德农业开发有限公司 | Method for cultivating and planting organic dendrobium candidum |
| CN105191769A (en) * | 2015-09-22 | 2015-12-30 | 苏州神元生物科技股份有限公司 | Cultivation method for increasing content of dendrobium officinale polysaccharides |
| CN105941106A (en) * | 2016-04-27 | 2016-09-21 | 庐江县天地禾生物技术有限公司 | Maintenance method for increasing content of dendrobium officinale polysaccharides |
| CN106258528A (en) * | 2016-08-09 | 2017-01-04 | 华南农业大学 | A kind of cultural method improving lemon mint volatile oil content |
| CN106359040A (en) * | 2016-08-26 | 2017-02-01 | 厦门市同安区坤灵中药种植专业合作社 | Novel planting method for solving water cracking phenomenon of dendrobium officinale |
| CN107354124A (en) * | 2016-05-10 | 2017-11-17 | 苏州天成新农生物科技有限公司 | A kind of dendrobium candidum cell scale evaluation method |
| CN107354123A (en) * | 2016-05-10 | 2017-11-17 | 张�杰 | A kind of plant cell scale evaluation method |
| CN108934924A (en) * | 2018-05-30 | 2018-12-07 | 江苏圣福来生态农业有限公司 | A kind of dendrobium candidum axenic culture seed broadcasts seedling culture medium and its application |
| CN109997622A (en) * | 2019-04-17 | 2019-07-12 | 广州市八斗农业科技有限公司 | The method for improving dendrobium candidum active constituent content |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101810140A (en) * | 2009-09-03 | 2010-08-25 | 四川万安石斛产业开发有限公司 | Method for propagating dendrobium candidum test-tube plantlets |
| CN102057867A (en) * | 2009-11-14 | 2011-05-18 | 斯金平 | Light control method for dendrobium officinale tissue culture |
-
2012
- 2012-04-05 CN CN2012100966520A patent/CN102726183A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101810140A (en) * | 2009-09-03 | 2010-08-25 | 四川万安石斛产业开发有限公司 | Method for propagating dendrobium candidum test-tube plantlets |
| CN102057867A (en) * | 2009-11-14 | 2011-05-18 | 斯金平 | Light control method for dendrobium officinale tissue culture |
Non-Patent Citations (3)
| Title |
|---|
| 付卫民等，: "《有色材料覆盖对心里美萝卜品质及产量的影响》", 《山东农业科学》, 30 June 2011 (2011-06-30) * |
| 高亭亭等: "《光质与种质对铁皮石斛种苗生长和有效成分的影响》", 《中国中药杂志》, 15 January 2012 (2012-01-15) * |
| 高亭亭等: "《光质与种质对铁皮石解试管苗生长的影响》", 《第十届全国药用植物及植物药学术研讨会》, 12 August 2011 (2011-08-12), pages 63 * |
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103931429A (en) * | 2014-03-14 | 2014-07-23 | 云南云琦丹生物科技有限公司 | Artificial light seedling culture method capable of promoting plant growth and differentiation |
| CN104026016A (en) * | 2014-06-20 | 2014-09-10 | 山东理工大学 | Method for producing dendrobium polysaccharides by inducing dendrobium candidum by jasmonic acid methyl ester |
| CN104285632A (en) * | 2014-09-10 | 2015-01-21 | 韦俊 | Cultivation method for improving polysaccharide content of Chinese yam |
| CN104855262B (en) * | 2015-06-05 | 2017-03-15 | 杭州正德农业开发有限公司 | The cultivation implantation methods of organic Herba Dendrobii |
| CN104855262A (en) * | 2015-06-05 | 2015-08-26 | 杭州正德农业开发有限公司 | Method for cultivating and planting organic dendrobium candidum |
| CN105191769A (en) * | 2015-09-22 | 2015-12-30 | 苏州神元生物科技股份有限公司 | Cultivation method for increasing content of dendrobium officinale polysaccharides |
| CN105191769B (en) * | 2015-09-22 | 2017-10-31 | 苏州神元生物科技股份有限公司 | A kind of cultural method for improving polysaccharide content of dendrobium candidum |
| CN105941106A (en) * | 2016-04-27 | 2016-09-21 | 庐江县天地禾生物技术有限公司 | Maintenance method for increasing content of dendrobium officinale polysaccharides |
| CN107354124A (en) * | 2016-05-10 | 2017-11-17 | 苏州天成新农生物科技有限公司 | A kind of dendrobium candidum cell scale evaluation method |
| CN107354123A (en) * | 2016-05-10 | 2017-11-17 | 张�杰 | A kind of plant cell scale evaluation method |
| CN106258528A (en) * | 2016-08-09 | 2017-01-04 | 华南农业大学 | A kind of cultural method improving lemon mint volatile oil content |
| CN106258528B (en) * | 2016-08-09 | 2021-12-28 | 华南农业大学 | Cultivation method for increasing content of lemon balm volatile oil |
| CN106359040A (en) * | 2016-08-26 | 2017-02-01 | 厦门市同安区坤灵中药种植专业合作社 | Novel planting method for solving water cracking phenomenon of dendrobium officinale |
| CN108934924A (en) * | 2018-05-30 | 2018-12-07 | 江苏圣福来生态农业有限公司 | A kind of dendrobium candidum axenic culture seed broadcasts seedling culture medium and its application |
| CN108934924B (en) * | 2018-05-30 | 2020-06-09 | 江苏圣福来生态农业有限公司 | Dendrobium officinale sterile culture seed seeding culture medium and application thereof |
| CN109997622A (en) * | 2019-04-17 | 2019-07-12 | 广州市八斗农业科技有限公司 | The method for improving dendrobium candidum active constituent content |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102726183A (en) | Cultivation method for improving polysaccharide content of dendrobium candidum | |
| CN104351054B (en) | One kind promotes iris Multiple Buds rapid propagation method using LED/light source | |
| CN104429949B (en) | A kind of method that utilization LED light source promotes iris tissue culture seedling rooting | |
| Manchanda et al. | Effect of activated charcoal, carbon sources and gelling agents on direct somatic embryogenesis and regeneration in sugarcane via leaf roll segments | |
| Jha et al. | Production of the alkaloids emetine and cephaeline in callus cultures of Cephaelis ipecacuanha | |
| Huang et al. | Optimization of induction, subculture conditions, and growth kinetics of Angelica sinensis (Oliv.) Diels callus | |
| CN110073835A (en) | A kind of cultural method of lycoris plants | |
| CN109329217B (en) | Method for artificially inoculating pine wood nematodes by using skin scratching method of tender tips of semi-annual Chinese red pine seedlings and application of method | |
| CN104651474B (en) | A kind of rapid identification method of muskmelon powdery mildew biological strain | |
| CN1918959A (en) | Sprout period authenticating method for tobacco mosaic resistantance | |
| Yang et al. | Variation in growth, photosynthesis and water-soluble polysaccharide of Cyclocarya paliurus under different light regimes | |
| CN102511368A (en) | Method for evaluating resistance of brassica plants on club root | |
| Kong et al. | Effect of supplemental lighting with red and blue light on the characters of container-growing seedlings of muskmelon | |
| Yan et al. | Efficient organogenesis and taxifolin production system from mature zygotic embryos and needles in larch | |
| CN104304002B (en) | Inducing method of lonicera confusa leaf adventitious bud | |
| CN103430839A (en) | Culture method of ellcalyplus grandis Hill ex Maid tissue culture seedling | |
| CN102939841B (en) | Method for improving anthocyanidin contents in purple white birch leaves | |
| CN206879684U (en) | A kind of work tree of dendrobium candidum possesses a person culture apparatus | |
| CN110050696B (en) | Method for obtaining high-polysaccharide high-yield dendrobium officinale through ion beam mutagenesis and application | |
| CN106305382A (en) | Three-dimensional dendrobium officinale culture substrate and preparation method thereof | |
| CN106605596A (en) | Method for mass propagation of lycoris aurea through somatic embryogenesis | |
| Chang et al. | New cultivation methods for Anoectochilus formosanus Hayata | |
| CN105794506B (en) | A method of improving Mongolian oak fern egagametophyte ratio | |
| CN105794648B (en) | The cultural method of the natural bog bilberry test tube strong sprout in Daxing'an Mountainrange | |
| Ilyas et al. | Analysis of physicochemical parameters to evaluate the mycelia growth of Pleurotus pulmonarius |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20121017 |