CN105191769B - A kind of cultural method for improving polysaccharide content of dendrobium candidum - Google Patents
A kind of cultural method for improving polysaccharide content of dendrobium candidum Download PDFInfo
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- CN105191769B CN105191769B CN201510607293.4A CN201510607293A CN105191769B CN 105191769 B CN105191769 B CN 105191769B CN 201510607293 A CN201510607293 A CN 201510607293A CN 105191769 B CN105191769 B CN 105191769B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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Abstract
The invention discloses a kind of cultural method for improving polysaccharide content of dendrobium candidum, comprise the following steps：Tissue culture seedling, seedling packing, seedling replanting, spraying are watered, temperature treatment, topdress management, illumination management, disease control and pest control.A kind of cultural method for raising polysaccharide content of dendrobium candidum that the present invention is provided, simple and reasonable steps, dendrobium candidum survival rate after transplanting is high, fast growth, and pest and disease damage is few, by to the humidity in greenhouse, temperature and the management of being controlled of illumination, make the day and night temperature in greenhouse convenient while ensure that effective accumulated temperature, under sufficient illumination condition, be conducive to photosynthesis, nutriment is conducive to transport and accumulate to cane, the polyoses content for the dendrobium candidum cultivated compared with prior art is obviously improved, the polyoses content for solving dendrobium candidum in the prior art is relatively low, the not high technical problem of its medical value, add the medical value of dendrobium candidum.
Description
Technical field
The present invention relates to the technical field of officinal dendrobium stem plantation, more particularly to a kind of cultivation for improving polysaccharide content of dendrobium candidum
Culture method.
Background technology
Dendrobium candidum is a kind of rare medicinal material, and its cold nature is sweet, light, the work(with nourishing Yin and clearing heat, nourishing the stomach to improve the production of body fluid
Effect, available for treatment with the acute and pharyngo-laryngitis chronica of yin deficiency syndrome, chronic bronchitis, tuberculosis, hyperthyroidism,
The illnesss such as diabetes, chronic hepatitis, atrophic gastritis, immunologic function syndrome and carcinosis radiotherapy and chemotherapy conditioning, and can strengthen
In old resistance against diseases.
Dendrobium officinale polysaccharide belongs to one kind of active polysaccharide, with multiple efficacies.The immunoregulation effect of human body is most
The collective effect of number active polysaccharide, modern pharmacology research analysis shows, the emplastic of dendrobium candidum stem is mainly water solubility
Polysaccharide, the ratio that content accounts for its dry weight is up to more than 25%, and it is a para-immunity reinforcing agent, to improving peripheral leukocytes and promotion
Lymphocyte, which produces shifter factor, has obvious effect, and Dendrobium officinale polysaccharide composition is thin with T, bone-marrow-derived lymphocyte, macrophage is increased
The remarkable effect of the immune cell function such as born of the same parents and NK (NK), can effectively suppress tumour, Dendrobium officinale polysaccharide has
Hypoglycemic effect, long-term taking Western medicine is hypoglycemic, insulin injection has very big side effect, and Dendrobium officinale polysaccharide is pure natural
Plant extraction, is had no side effect, different from oral hypoglycemic sulfonylurea, and it is acted on without reduction euglycemia, is not also improved
Serum insulin level, and there is repair to islet cells, while also there is effect hypoglycemic outside pancreas, by suppressing kidney
Hepatic glycogenolytic caused by upper parathyrine and promotion hepatic glycogen synthesis, reach the purpose of reduction blood glucose value, in addition, Dendrobium officinale polysaccharide
Anti-oxidant, anti-aging effects.
At present, in officinal dendrobium stem plantation technology, the speed of growth of dendrobium candidum is very fast, and in dendrobium candidum polyoses content compared with
It is low, reduce its medical value, it is therefore necessary to propose a kind of cultural method for improving polysaccharide content of dendrobium candidum.
The content of the invention
It is an object of the invention to overcome above-mentioned the deficiencies in the prior art, there is provided a kind of raising polysaccharide content of dendrobium candidum
Cultural method, it aims to solve the problem that the polyoses content of dendrobium candidum in the prior art is relatively low, the not high technical problem of its medical value.
To achieve the above object, the present invention proposes a kind of cultural method for improving polysaccharide content of dendrobium candidum, including such as
Lower step：
Step 1: tissue culture seedling：Wild dendrobium candidum seedling is chosen, explant culture, bud to be turned out is carried out
Or after leaf bud, bud or leaf bud are put into progress sterile propagation culture, 25 DEG C~28 DEG C of cultivation temperature, daily illumination in culture medium
9~10 hours, intensity of illumination was 32.4~34.2 μm of ol.m-2.s-1；
Step 2: seedling dispenses：When growth of seedling to 2cm or so, it is dispensed into blake bottle and is cultivated, each culture
4~6 plants of seedling are cultivated in bottle；
Step 3: seedling replanting：As a length of 0.5~1cm of the seedling root in blake bottle, it is one clump to choose 3 plants of seedling, often
Strain seedling 4~5cm of height of seedling, at least containing 5 blades, 5 corings, first cleans the culture medium of seedling root, then use with clear water
0.1% potassium permanganate dilution spray disinfectant, is then colonized in the matrix into greenhouse, greatly with the cm of 20cm × 20 distance between rows and hills
Light irradiation apparatus, ventilation unit and humidity conditioner are set in canopy；
Step 4: spraying is watered：The seedling just transplanted to greenhouse is watered in the way of spraying, waters to hand and grabs base
Matter has wet sense but not dripped, and transplants in one week, controls the humidity in greenhouse to be maintained at 90% or so, one week with humidity conditioner
Afterwards, the humidity in control greenhouse is maintained at 70~80%, and is aerated using ventilation unit；
Step 5: temperature treatment：Day temperature control in greenhouse is at 25~30 DEG C, and night temperatures are controlled 18~20
DEG C, during summer high temperature, cooled in the form of spraying, and ventilation and heat, during winter low temperature, greenhouse is coated with double-plastic
Sealing, prevents plant freeze injury；
Step 6: management of topdressing：After seedling replanting two weeks, when seedling grows new root, foliage-spray is made with alga fertilizer, seedling is treated
When growing at slightly larger, foliage spray is carried out with the general fertilizer of doctor Lu, the root compound organic fertilizer material of nutrient >=45% sprays
Apply, the amount of spraying of topdressing is few, carry out once every two weeks；
Step 7: illumination management：Greenhouse is sheltered from heat or light using 70% shade net, and carries out illumination to plant using light irradiation apparatus
Management, it is 80~120 μm of ol.m to control the intensity of illumination in greenhouse-2.s-1；
Step 8: disease control：Monthly sprayed with Bordeaux mixture and carry out foliar control；
Step 9: pest control：One layer of fly net of greenhouse outer cladding, is entered by way of manually being combined with medicine to greenhouse
Row insect pest is removed, and prevents plant to wither.
Preferably, the matrix in the step 3 by bark sawdust into bits, and pass through boiling water 1 hour, natural cooling
Immersion 2~3 days, then dry and form, the filling thickness of greenhouse mesostroma is more than 8cm.
Preferably, the water pH value watered of being sprayed in the step 4 is controlled between 4.5~5.5.
Preferably, disease control uses 800~1000 times of liquid of equivalent formula Bordeaux mixture in the step 8.
Beneficial effects of the present invention：Compared with prior art, a kind of raising polysaccharide content of dendrobium candidum that the present invention is provided
Cultural method, simple and reasonable steps, dendrobium candidum survival rate after transplanting is high, fast growth, and pest and disease damage is few, by right
Humidity, temperature and the management of being controlled of illumination in greenhouse, it is ensured that make the day and night temperature in greenhouse while effective accumulated temperature
Conveniently, under sufficient illumination condition, be conducive to photosynthesis, be conducive to nutriment to transport and accumulate to cane, improve
Polyoses content in dendrobium candidum, so as to improve the medical value of dendrobium candidum.
The feature and advantage of the present invention will be described in detail by embodiment.
Embodiment
To make the object, technical solutions and advantages of the present invention of greater clarity, below by embodiment, the present invention is entered
Row is further described.However, it should be understood that the specific embodiments described herein are merely illustrative of the present invention, and without
In limitation the scope of the present invention.In addition, in the following description, eliminate the description to known features and technology, so that avoid need not
Obscure idea of the invention in strategic point.
The embodiment of the present invention provides a kind of cultural method for improving polysaccharide content of dendrobium candidum, comprises the following steps：
Step 1: tissue culture seedling：Wild dendrobium candidum seedling is chosen, explant culture, bud to be turned out is carried out
Or after leaf bud, bud or leaf bud are put into progress sterile propagation culture, 25 DEG C~28 DEG C of cultivation temperature, daily illumination in culture medium
9~10 hours, intensity of illumination was 32.4~34.2 μm of ol.m-2.s-1；
Step 2: seedling dispenses：When growth of seedling to 2cm or so, it is dispensed into blake bottle and is cultivated, each culture
4~6 plants of seedling are cultivated in bottle；
Step 3: seedling replanting：As a length of 0.5~1cm of the seedling root in blake bottle, it is one clump to choose 3 plants of seedling, often
Strain seedling 4~5cm of height of seedling, at least containing 5 blades, 5 corings, first cleans the culture medium of seedling root, then use with clear water
0.1% potassium permanganate dilution spray disinfectant, is then colonized in the matrix into greenhouse, greatly with the cm of 20cm × 20 distance between rows and hills
Light irradiation apparatus, ventilation unit and humidity conditioner are set in canopy；
Step 4: spraying is watered：The seedling just transplanted to greenhouse is watered in the way of spraying, waters to hand and grabs base
Matter has wet sense but not dripped, and transplants in one week, controls the humidity in greenhouse to be maintained at 90% or so, one week with humidity conditioner
Afterwards, the humidity in control greenhouse is maintained at 70~80%, and is aerated using ventilation unit；
Step 5: temperature treatment：Day temperature control in greenhouse is at 25~30 DEG C, and night temperatures are controlled 18~20
DEG C, during summer high temperature, cooled in the form of spraying, and ventilation and heat, during winter low temperature, greenhouse is coated with double-plastic
Sealing, prevents plant freeze injury；
Step 6: management of topdressing：After seedling replanting two weeks, when seedling grows new root, foliage-spray is made with alga fertilizer, seedling is treated
When growing at slightly larger, foliage spray is carried out with the general fertilizer of doctor Lu, the root compound organic fertilizer material of nutrient >=45% sprays
Apply, the amount of spraying of topdressing is few, carry out once every two weeks；
Step 7: illumination management：Greenhouse is sheltered from heat or light using 70% shade net, and carries out illumination to plant using light irradiation apparatus
Management, it is 80~120 μm of ol.m to control the intensity of illumination in greenhouse-2.s-1；
Step 8: disease control：Monthly sprayed with Bordeaux mixture and carry out foliar control；
Step 9: pest control：One layer of fly net of greenhouse outer cladding, is entered by way of manually being combined with medicine to greenhouse
Row insect pest is removed, and prevents plant to wither.
Matrix in the step 3, into bits, and passes through boiling water 1 hour, natural cooling immersion 2~3 by bark sawdust
My god, then dry and form, the filling thickness of greenhouse mesostroma is more than the water pH value control watered of being sprayed in 8cm, the step 4 and existed
Between 4.5~5.5, disease control uses 800~1000 times of liquid of equivalent formula Bordeaux mixture in the step 8.
The dendrobium candidum that the embodiment of the present invention is cultivated significantly is carried compared with the dendrobium candidum that prior art is cultivated, the speed of growth
Height, and plant is larger, survival rate is high, and pest and disease damage is less.When the dendrobium candidum that the embodiment of the present invention is cultivated enters picking time,
The dendrobium candidum that the dendrobium candidum cultivated of the embodiment of the present invention and prior art of same time period transplanting are cultivated is won, is chosen
The more consistent dendrobium candidum of growing way is selected to carry out measurement of the polysaccharide content, polyoses content is carried out using the method for phenol-sulfuric acid colorimetric
Determine.
Measurement result is it has been found by contrast that the average value of polyoses content is obvious in the dendrobium candidum that the embodiment of the present invention is cultivated
The polyoses content average value for the dendrobium candidum cultivated higher than prior art, shows that the present invention can improve the polysaccharide in dendrobium candidum
Content, improves the medical value of dendrobium candidum.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
Any modification, equivalent substitution or improvement made within refreshing and principle etc., should be included in the scope of the protection.
Claims (4)
1. a kind of cultural method for improving polysaccharide content of dendrobium candidum, it is characterised in that：Comprise the following steps：
Step 1: tissue culture seedling：Wild dendrobium candidum seedling is chosen, explant culture, bud or leaf to be turned out is carried out
After bud, bud or leaf bud are put into culture medium and carry out sterile propagation culture, 25 DEG C~28 DEG C of cultivation temperature, daily illumination 9~
10 hours, intensity of illumination was 32.4~34.2 μm of ol.m-2.s-1；
Step 2: seedling dispenses：When growth of seedling to 2cm or so, it is dispensed into blake bottle and is cultivated, in each blake bottle
Cultivate 4~6 plants of seedling；
Step 3: seedling replanting：As a length of 0.5~1cm of the seedling root in blake bottle, it is one clump, every plant of children to choose 3 plants of seedling
Seedling 4~5cm of height of seedling, at least containing 5 blades, 5 corings, first cleans the culture medium of seedling root with clear water, then with 0.1%
Potassium permanganate dilution spray disinfectant, is then colonized in the matrix into greenhouse with the cm of 20cm × 20 distance between rows and hills, is set in greenhouse
Put light irradiation apparatus, ventilation unit and humidity conditioner；
Step 4: spraying is watered：Watered to just transplanting to the seedling of greenhouse in the way of spraying, water and grab matrix to hand and have
Wet sense but do not drip, transplant in one week, control the humidity in greenhouse to be maintained at behind 90% or so, one week with humidity conditioner, control
Humidity in greenhouse processed is maintained at 70~80%, and is aerated using ventilation unit；
Step 5: temperature treatment：Day temperature control in greenhouse is at 25~30 DEG C, and night temperatures are controlled in 18~20 DEG C, summer
During Ji Gaowen, cooled in the form of spraying, and ventilation and heat, during winter low temperature, greenhouse is coated with double-plastic and sealed,
Prevent plant freeze injury；
Step 6: management of topdressing：After seedling replanting two weeks, when seedling grows new root, foliage-spray is made with alga fertilizer, treats that seedling is growed
When slightly larger, foliage spray is carried out with the general fertilizer of doctor Lu, root is sprayed with the compound organic fertilizer material of nutrient >=45%, chased after
The fertilizer amount of spraying is few, carries out once every two weeks；
Step 7: illumination management：Greenhouse is sheltered from heat or light using 70% shade net, and carries out illumination pipe to plant using light irradiation apparatus
Reason, it is 80~120 μm of ol.m to control the intensity of illumination in greenhouse-2.s-1；
Step 8: disease control：Monthly sprayed with Bordeaux mixture and carry out foliar control；
Step 9: pest control：One layer of fly net of greenhouse outer cladding, worm is carried out by way of manually being combined with medicine to greenhouse
Evil is removed, and prevents plant to wither.
2. a kind of cultural method for improving polysaccharide content of dendrobium candidum as claimed in claim 1, it is characterised in that：The step
Matrix in three is by bark sawdust into bits, and by boiling water 1 hour, natural cooling was soaked 2~3 days, then dries and form, greenhouse
The filling thickness of mesostroma is more than 8cm.
3. a kind of cultural method for improving polysaccharide content of dendrobium candidum as claimed in claim 1, it is characterised in that：The step
The water pH value watered of being sprayed in four is controlled between 4.5~5.5.
4. a kind of cultural method for improving polysaccharide content of dendrobium candidum as claimed in claim 1, it is characterised in that：The step
Disease control uses 800~1000 times of liquid of equivalent formula Bordeaux mixture in eight.
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| CN105941106B (en) * | 2016-04-27 | 2019-02-19 | 庐江县天地禾生物技术有限公司 | A kind of maintenance process improving polysaccharide content of dendrobium candidum |
| CN108739105A (en) * | 2018-05-18 | 2018-11-06 | 广西中医药大学 | A method of improving polysaccharide content of dendrobium candidum |
| CN114306590A (en) * | 2021-12-31 | 2022-04-12 | 皖西学院 | Veterinary vaccine diluent with immunity enhancement effect and preparation method and application thereof |
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Inventor after: He Yunfang Inventor after: Teng Shiyuan Inventor after: Zheng Bingsong Inventor after: Ren Wei Inventor after: Wang Yang Inventor after: Zhong Shuanglin Inventor before: Teng Shiyuan Inventor before: He Yunfang Inventor before: Zheng Bingsong Inventor before: Ren Wei Inventor before: Wang Yang Inventor before: Zhong Shuanglin |