CN105941106B - A kind of maintenance process improving polysaccharide content of dendrobium candidum - Google Patents
A kind of maintenance process improving polysaccharide content of dendrobium candidum Download PDFInfo
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- CN105941106B CN105941106B CN201610267395.0A CN201610267395A CN105941106B CN 105941106 B CN105941106 B CN 105941106B CN 201610267395 A CN201610267395 A CN 201610267395A CN 105941106 B CN105941106 B CN 105941106B
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- dendrobium candidum
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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Abstract
The invention discloses a kind of maintenance processes for improving polysaccharide content of dendrobium candidum, suitable environment is provided within the growth period of 4~September dendrobium candidum biomass Rapid Accumulation, the accelerated accumulation for promoting dendrobium candidum biomass utilizes the fast lifting of polysaccharide content of dendrobium candidum in light filling measure and adverse circumstance environmental Kuznets Curves appropriate promotion dendrobium candidum in 10~December.Maintenance process of the present invention not only can be improved the content of Dendrobium officinale polysaccharide but also can ensure the yield of dendrobium candidum, guarantee the quality of dendrobium candidum product.The method of the present invention is simple and efficient, it convenient for practical application popularization, is detected, after sampling the method for the present invention conserves officinal dendrobium stem plantation kind 1 year, polysaccharide content of dendrobium candidum in dendrobium candidum can reach 35.69%~42.18%, much higher than 25% specified in China's pharmacopeia.
Description
Technical field
The invention belongs to Plant Biotechnology technical field, specifically a kind of maintenance side for improving polysaccharide content of dendrobium candidum
Method.
Background technique
Dendrobium candidum (Dendrobium officinale) it is orchid family Dendrobium herbaceos perennial, it is commonly called as ribbed hedyotis herb,
It is mainly distributed on the ground such as Zhejiang, Anhui, Guangdong, Guangxi.Dendrobium candidum has nourishing the stomach to improve the production of body fluid, strong negative strengthening the essence, balance the five internal organs, drop three
It is high, antitumor, improve the immunity of the human body and other effects, be listed in " Taoist Scriptures " first of " Chinese nine big mesonas ", dendrobium candidum
Because its unique effect is favored by vast health care occurrences in human life, the civil laudatory title for being known as " help mesona " and " Radix Rhodiolae ".Iron
Skin dendrobium nobile field natural breed rate is extremely low, in addition the mad excavation of people, and dendrobium officinale resource is endangered at present,
Artificial cultivation technique comparative maturity.
Modern pharmacological research finds that active constituent is mainly Dendrobium officinale polysaccharide, dendrobine, dendrobium nobile phenanthrene in dendrobium candidum
Deng.Wherein Dendrobium officinale polysaccharide belongs to immunopotentiator, has effects that increase immunocyte and macrophage immunity activity,
And it can effectively inhibit tumour.The height of polysaccharide content of dendrobium candidum, which is directly becoming, judges referring mainly to for dendrobium candidum quality
It marks, the national standard of Dendrobium officinale polysaccharide is scheduled on 25% in Pharmacopoeias of People's Republic of China in 2010.The medicines such as Dendrobium officinale polysaccharide
It is its secondary metabolite with ingredient, is not necessary to itself growth and development, only in the case where it encounters adverse circumstance
It can just generate.Dendrobium candidum is relatively high to moisture, illumination, temperature requirement during artificial cultivation as orchid.But
In modern artificial cultivation, farmer largely uses liquid manure to covet yield, to the growing environment of dendrobium candidum
Excessively it is suitable for, the requirement of national standard is not achieved in polysaccharide content of dendrobium candidum in the dendrobium candidum for causing more farmers to be planted, and causes
The amount of having is detained without the dendrobium candidum product of matter, the event that is difficult to sell takes place frequently, and it is necessary to develop to can be improved iron sheet stone thus
The maintenance process of dry measure used in former times polyoses content.
Summary of the invention
The technical problem to be solved by the present invention is in view of the deficiencies of the prior art, it is more provide a kind of raising dendrobium candidum
The maintenance process of sugared content can either effectively improve the quality of dendrobium candidum, in turn, ensure that the yield of dendrobium candidum, side of the present invention
Method is simple and efficient, and is promoted convenient for practical application.
The technical scheme of the invention to solve the technical problem is: a kind of improve supporting for polysaccharide content of dendrobium candidum
Maintaining method, comprising the following steps:
1) selection of officinal dendrobium stem plantation kind:
For 1~2 year domesticated seedlings of dendrobium candidum for selecting Zhejiang or Anhui to plant as cultivar, cultural area is Zhejiang
Or Anhui, cultivation mode are the bed planting of disjunctor greenhouse；
2) maintenance of officinal dendrobium stem plantation kind First Year:
1~2 year domesticated seedlings of dendrobium candidum are colonized in disjunctor greenhouse seedbed at the beginning of annual 4 months, after field planting, in 4~May
By air humidity control in greenhouse 70~90%, 60~70%, temperature is controlled at 10~25 DEG C the control of sunshade rate, and carries out it
His routine servicing；In 6~July by air humidity control in greenhouse 60~80%, 65~75%, temperature controls the control of sunshade rate
At 15~35 DEG C, and carry out other routine servicings；In 8~September by air humidity control is 50~60% in greenhouse, sunshade rate control
70~80%, temperature is controlled at 20~33 DEG C system, and carries out other routine servicings；In seedbed bottom and greenhouse management at the beginning of 10 months
Aisle is laid with Reflecting mulch film, and above the seedbed 3500~4800Lux of the place 40~60cm installation full spectrum Plant Tissue Breeding
Special lamp, the quantity for the full spectrum Plant Tissue Breeding special lamp installed above seedbed per acre are 60~80, full spectrum plant
The lighting time of tissue cultures special lamp is turned on light from 16 points to 21 points daily in 10~December, will be empty in greenhouse in 10~December
Gas humid control is 30~50%, and 30~40%, temperature is controlled at -4~12 DEG C the control of sunshade rate, and it is daily feeding to carry out other
Shield, until completing the maintenance to officinal dendrobium stem plantation kind First Year by the end of December；
3) harvesting of officinal dendrobium stem plantation kind:
Maintenance 1~2 time of step 2 is repeated, completes that the entire maintenance processes of officinal dendrobium stem plantation kind can be carried out
The harvesting of officinal dendrobium stem plantation kind.Since dendrobium candidum is herbaceos perennial, usual maintenance of plant can be adopted for 2~3 years
It receives, therefore after First Year maintenance, subsequent conserve again can be completed to the entire feeding of officinal dendrobium stem plantation kind for 1~2 year
Shield process simultaneously harvests.
Annual 4~September is the growth period of dendrobium candidum biomass accelerated accumulation, and maintenance process of the present invention is according to different months
External environment feature controls air humidity, sunshade rate and temperature range in greenhouse, provides suitable ring for the growth of dendrobium candidum
Border promotes the accelerated accumulation of dendrobium candidum biomass.With climate change, the outside air temperature in 10~December is gradually decreased, minimum
In subzero, and dendrobium candidum is easy frostbite at -5 DEG C of temperature or less, is freezed to death due to its biological nature.Present invention maintenance side
In method, suitably increase the temperature difference, light application time and the intensity of illumination in greenhouse in annual 10~December, is mentioned for the growth of dendrobium candidum
For adverse circumstance environment appropriate, dendrobium candidum is allowed to be in -4~12 DEG C of temperature range, which is not Growth of Dendrobium candidum
Preference temperature scope, but a kind of adverse circumstance temperature appropriate, it is sufficient to make dendrobium candidum be unlikely to frost bitten or freeze to death, tie simultaneously
The control for closing light application time and intensity of illumination, finally makes the polysaccharide content of dendrobium candidum in dendrobium candidum obtain accelerated accumulation, mention
It rises.
Other described routine servicings are conventional organic farming maintenance, including watering, weeding and fertilising.
Compared with the prior art, the advantages of the present invention are as follows: the present invention improves the maintenance process of polysaccharide content of dendrobium candidum,
Suitable environment is provided within the growth period of 4~September dendrobium candidum biomass Rapid Accumulation, promotes the fast of dendrobium candidum biomass
Speed accumulation promotes Dendrobium officinale polysaccharide in dendrobium candidum to contain in 10~December using light filling measure and adverse circumstance environmental Kuznets Curves appropriate
The fast lifting of amount.Maintenance process of the present invention not only can be improved the content of Dendrobium officinale polysaccharide but also can ensure the yield of dendrobium candidum,
Guarantee the quality of dendrobium candidum product.The method of the present invention is simple and efficient, and is promoted, is detected convenient for practical application, the sampling present invention
After method conserves officinal dendrobium stem plantation kind 1 year, polysaccharide content of dendrobium candidum in dendrobium candidum can reach 35.69%~
42.18%, much higher than 25% specified in China's pharmacopeia.
Specific embodiment
Present invention is further described in detail with reference to embodiments.
The maintenance process of the raising polysaccharide content of dendrobium candidum of embodiment 1, comprising the following steps:
1) selection of officinal dendrobium stem plantation kind:
For the 1 year domesticated seedlings of dendrobium candidum for selecting Zhejiang to plant as cultivar, cultural area is Ningbo of Zhejiang, cultivates mould
Formula is the bed planting of disjunctor greenhouse；
2) maintenance of officinal dendrobium stem plantation kind First Year:
1 year domesticated seedlings of dendrobium candidum of selection are colonized by April 2nd, 2015 in disjunctor greenhouse seedbed, after field planting,
4~May, 60~65%, temperature was controlled at 10~22 DEG C the control of sunshade rate by air humidity control in greenhouse 70~90%, and
Carry out other routine servicings；In 6~July by air humidity control in greenhouse 60~80%, the control of sunshade rate is 65~70%, temperature
Degree control carries out other routine servicings at 15~35 DEG C；Air humidity control in greenhouse is hidden 50~60% in 8~September
70~75%, temperature is controlled at 20~33 DEG C, and carries out other routine servicings for positive rate control；In seedbed bottom and greatly at the beginning of 10 months
Canopy manages aisle and is laid with Reflecting mulch film, and the full spectrum Plant Tissue Breeding of 3500 Lux of the place 40 cm installation is special above the seedbed
With lamp, the quantity for the full spectrum Plant Tissue Breeding special lamp installed above seedbed per acre is 60, full spectrum plant tissue training
The lighting time of special lamp is supported in 10~December, is turned on light daily from 16 points to 21 points, in 10~December by air humidity in greenhouse
Control is 30~50%, and the control of sunshade rate is 30~40%, and temperature is controlled at 0~12 DEG C, and carries out other routine servicings, until December
Bottom is to complete maintenance to officinal dendrobium stem plantation kind First Year, in extraction on December 28th, 2015 dendrobium candidum sample, according to
Pharmacopeia detection method carries out the detection of polysaccharide content of dendrobium candidum within 2015, and measuring polyoses content is 35.69%.；
3) harvesting of officinal dendrobium stem plantation kind:
Maintenance 2 times of step 2 are repeated, complete that iron sheet can be carried out to the entire maintenance processes of officinal dendrobium stem plantation kind
The harvesting of dendrobe cultivation kind.
The maintenance process of the raising polysaccharide content of dendrobium candidum of embodiment 2, comprising the following steps:
1) selection of officinal dendrobium stem plantation kind:
For the 2 years domesticated seedlings of dendrobium candidum for selecting Zhejiang to plant as cultivar, cultural area is Lujiang, Anhui, cultivates mould
Formula is the bed planting of disjunctor greenhouse；
2) maintenance of officinal dendrobium stem plantation kind First Year:
2 years domesticated seedlings of dendrobium candidum of selection are colonized by April 2nd, 2015 in disjunctor greenhouse seedbed, after field planting,
4~May, 65~70%, temperature was controlled at 10~25 DEG C the control of sunshade rate by air humidity control in greenhouse 70~90%, and
Carry out other routine servicings；In 6~July by air humidity control in greenhouse 60~80%, the control of sunshade rate is 70~75%, temperature
Degree control carries out other routine servicings at 15~35 DEG C；Air humidity control in greenhouse is hidden 50~60% in 8~September
75~80%, temperature is controlled at 20~33 DEG C, and carries out other routine servicings for positive rate control；In seedbed bottom and greatly at the beginning of 10 months
Canopy manages aisle and is laid with Reflecting mulch film, and the full spectrum Plant Tissue Breeding of 4800 Lux of the place 60 cm installation is special above the seedbed
With lamp, the quantity for the full spectrum Plant Tissue Breeding special lamp installed above seedbed per acre is 80, full spectrum plant tissue training
The lighting time of special lamp is supported in 10~December, is turned on light daily from 16 points to 21 points, in 10~December by air humidity in greenhouse
Control is 30~50%, and the control of sunshade rate is 30~40%, and temperature is controlled at -4~12 DEG C, and carries out other routine servicings, until 12
The end of month is that the maintenance completed to officinal dendrobium stem plantation kind First Year is pressed in extraction on December 30th, 2015 dendrobium candidum sample
The detection of polysaccharide content of dendrobium candidum is carried out according to pharmacopeia detection method in 2015, measuring polyoses content is 41.52%；
3) harvesting of officinal dendrobium stem plantation kind:
Maintenance 1 time of step 2 is repeated, completes that iron sheet can be carried out to the entire maintenance processes of officinal dendrobium stem plantation kind
The harvesting of dendrobe cultivation kind.
The maintenance process of the raising polysaccharide content of dendrobium candidum of embodiment 3, comprising the following steps:
1) selection of officinal dendrobium stem plantation kind:
For the 1 year domesticated seedlings of dendrobium candidum for selecting Anhui to plant as cultivar, cultural area is Zhejiang Hangzhou, cultivates mould
Formula is the bed planting of disjunctor greenhouse；
2) maintenance of officinal dendrobium stem plantation kind First Year:
2 years domesticated seedlings of dendrobium candidum of selection are colonized by April 3rd, 2015 in disjunctor greenhouse seedbed, after field planting,
4~May, 60~70%, temperature was controlled at 10~25 DEG C the control of sunshade rate by air humidity control in greenhouse 70~90%, and
Carry out other routine servicings；In 6~July by air humidity control in greenhouse 60~80%, the control of sunshade rate is 70~75%, temperature
Degree control carries out other routine servicings at 15~35 DEG C；Air humidity control in greenhouse is hidden 50~60% in 8~September
75~80%, temperature is controlled at 20~33 DEG C, and carries out other routine servicings for positive rate control；In seedbed bottom and greatly at the beginning of 10 months
Canopy manages aisle and is laid with Reflecting mulch film, and the full spectrum Plant Tissue Breeding of 4800 Lux of the place 60 cm installation is special above the seedbed
With lamp, the quantity for the full spectrum Plant Tissue Breeding special lamp installed above seedbed per acre is 80, full spectrum plant tissue training
The lighting time of special lamp is supported in 10~December, is turned on light daily from 16 points to 21 points, in 10~December by air humidity in greenhouse
Control is 30~50%, and the control of sunshade rate is 30~40%, and temperature is controlled at -1~12 DEG C, and carries out other routine servicings, until 12
The end of month is that the maintenance completed to officinal dendrobium stem plantation kind First Year is pressed in extraction on December 30th, 2015 dendrobium candidum sample
The detection of polysaccharide content of dendrobium candidum is carried out according to pharmacopeia detection method in 2015, measuring polyoses content is 38.25%；
3) harvesting of officinal dendrobium stem plantation kind:
Maintenance 1 time of step 2 is repeated, completes that iron sheet can be carried out to the entire maintenance processes of officinal dendrobium stem plantation kind
The harvesting of dendrobe cultivation kind.
The maintenance process of the raising polysaccharide content of dendrobium candidum of embodiment 4, comprising the following steps:
1) selection of officinal dendrobium stem plantation kind:
For the 2 years domesticated seedlings of dendrobium candidum for selecting Anhui to plant as cultivar, cultural area is Huoshan, cultivates mould
Formula is the bed planting of disjunctor greenhouse；
2) maintenance of officinal dendrobium stem plantation kind First Year:
2 years domesticated seedlings of dendrobium candidum of selection are colonized by April 3rd, 2015 in disjunctor greenhouse seedbed, after field planting,
4~May, 60~65%, temperature was controlled at 10~22 DEG C the control of sunshade rate by air humidity control in greenhouse 70~90%, and
Carry out other routine servicings；In 6~July by air humidity control in greenhouse 60~80%, the control of sunshade rate is 65~70%, temperature
Degree control carries out other routine servicings at 15~35 DEG C；Air humidity control in greenhouse is hidden 50~60% in 8~September
70~75%, temperature is controlled at 20~33 DEG C, and carries out other routine servicings for positive rate control；In seedbed bottom and greatly at the beginning of 10 months
Canopy manages aisle and is laid with Reflecting mulch film, and the full spectrum Plant Tissue Breeding of 3500 Lux of the place 40 cm installation is special above the seedbed
With lamp, the quantity for the full spectrum Plant Tissue Breeding special lamp installed above seedbed per acre is 60, full spectrum plant tissue training
The lighting time of special lamp is supported in 10~December, is turned on light daily from 16 points to 21 points, in 10~December by air humidity in greenhouse
Control is 30~50%, and the control of sunshade rate is 30~40%, and temperature is controlled at -4~10 DEG C, and carries out other routine servicings, until 12
The end of month is that the maintenance completed to officinal dendrobium stem plantation kind First Year is pressed in extraction on December 30th, 2015 dendrobium candidum sample
The detection of polysaccharide content of dendrobium candidum is carried out according to pharmacopeia detection method in 2015, measuring polyoses content is 42.18%；
3) harvesting of officinal dendrobium stem plantation kind:
Maintenance 2 times of step 2 are repeated, complete that iron sheet can be carried out to the entire maintenance processes of officinal dendrobium stem plantation kind
The harvesting of dendrobe cultivation kind.
Other routine servicings taken in embodiments above are, including watering, weeding and fertilising etc., specifically may be used
It is carried out referring to existing conventional organic farming maintenance means and mode.
Claims (2)
1. a kind of maintenance process for improving polysaccharide content of dendrobium candidum, it is characterised in that the following steps are included:
1) selection of officinal dendrobium stem plantation kind:
Select 1~2 year domesticated seedlings of dendrobium candidum of Zhejiang perhaps Anhui plantation as cultivar cultural area for Zhejiang or
Anhui, cultivation mode are the bed planting of disjunctor greenhouse；
2) maintenance of officinal dendrobium stem plantation kind First Year:
1~2 year domesticated seedlings of dendrobium candidum are colonized in disjunctor greenhouse seedbed at the beginning of annual 4 months, it, will be big in 4~May after field planting
Air humidity control is 70~90% in canopy, and 60~70%, temperature is controlled at 10~25 DEG C the control of sunshade rate, and carries out other days
Often maintenance；In 6~July by air humidity control in greenhouse 60~80%, 65~75%, temperature is controlled 15 the control of sunshade rate
~35 DEG C, and carry out other routine servicings；In 8~September by air humidity control in greenhouse 50~60%, the control of sunshade rate exists
70~80%, temperature is controlled at 20~33 DEG C, and carries out other routine servicings；Aisle is managed in seedbed bottom and greenhouse at the beginning of 10 months
It is laid with Reflecting mulch film, and the full spectrum Plant Tissue Breeding of 3500~4800Lux of the place 40~60cm installation is dedicated above the seedbed
Lamp, the quantity for the full spectrum Plant Tissue Breeding special lamp installed above seedbed per acre are 60~80, full spectrum plant tissue
The lighting time of special lamp is cultivated in 10~December, is turned on light daily from 16 points to 21 points, it is in 10~December that air in greenhouse is wet
Degree control is 30~50%, and 30~40%, temperature is controlled at -4~12 DEG C the control of sunshade rate, and carries out other routine servicings, until
The maintenance to officinal dendrobium stem plantation kind First Year is completed by the end of December；
3) harvesting of officinal dendrobium stem plantation kind:
Maintenance 1~2 time of step 2 is repeated, completes that iron sheet can be carried out to the entire maintenance processes of officinal dendrobium stem plantation kind
The harvesting of dendrobe cultivation kind.
2. a kind of maintenance process for improving polysaccharide content of dendrobium candidum according to claim 1, it is characterised in that described
Other routine servicings are conventional organic farming maintenance, including watering, weeding and fertilising.
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| CN106613654A (en) * | 2016-12-12 | 2017-05-10 | 浙江大学宁波理工学院 | Regulation method of increasing taxus polysaccharide content in Taxus Chinensis Var.Mairei leaves |
| CN109997622A (en) * | 2019-04-17 | 2019-07-12 | 广州市八斗农业科技有限公司 | The method for improving dendrobium candidum active constituent content |
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| CN101999290B (en) * | 2010-09-14 | 2011-11-30 | 吴江市苗圃集团有限公司 | Cultivation method of dendrobium candidum |
| CN102726183A (en) * | 2012-01-20 | 2012-10-17 | 浙江农林大学 | Cultivation method for improving polysaccharide content of dendrobium candidum |
| CN104982169A (en) * | 2015-06-10 | 2015-10-21 | 安徽森亚生态农林有限公司 | Dendrobium officinale cultivation technology |
| CN105191769B (en) * | 2015-09-22 | 2017-10-31 | 苏州神元生物科技股份有限公司 | A kind of cultural method for improving polysaccharide content of dendrobium candidum |
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