CN106613654A

CN106613654A - Regulation method of increasing taxus polysaccharide content in Taxus Chinensis Var.Mairei leaves

Info

Publication number: CN106613654A
Application number: CN201611137680.7A
Authority: CN
Inventors: 阮晓; 王强
Original assignee: Ningbo Institute of Technology of ZJU
Current assignee: Ningbo Institute of Technology of ZJU
Priority date: 2016-12-12
Filing date: 2016-12-12
Publication date: 2017-05-10

Abstract

The invention discloses a regulation method of increasing taxus polysaccharide content in Taxus Chinensis Var.Mairei leaves. The regulation method comprises the following steps: before harvesting for 4-6 days, irrigating seedlings with a NaCl solution of proper concentration, and collecting Taxus Chinensis Var.Mairei leaves after watering for 4-6 days, so as to obtain Taxus Chinensis Var.Mairei leaves with increased taxus polysaccharide content. The method is simple and practical, the increasing effect of the taxus polysaccharide content is obvious, and the taxus polysaccharide content can be increased by three times.

Description

Improve the regulation and control method of yew amylose in leaves of Taxus mairei

Technical field

The present invention relates to the control technique of medicinal plant active component, and in particular to improve Chinese yew in leaves of Taxus mairei The regulation and control method of polysaccharide.

Background technology

Southerm yew（T chinesis var. Mairei）It is height above sea level 800-1600 m Subtropical Evergreen Broad-leaf Forests, often Green and Deciduous Broad Leaved Mixed Forest characteristic species, are distributed in the mountain region small stream of the province such as China Yangtze river basin, Nanling mountain and Henan, Shaanxi Paddy.The plant species natural propagation updating ability is extremely low, and in addition the mankind are more in habitat to deposit in occasional species form to its predatoriness exploitation Existing population quantity is on a declining curve, is listed in country-level Top-rated protected wild plants within 1999.Chinese yew medicinal function It is recorded in earliest《Compendium of Materia Medica》, treatment cholera, typhoid fever, diuresis, the disease such as stimulate the menstrual flow.Yew amylose is with other medicinal plant polysaccharides Equally, with extensive pharmacotoxicological effect, mainly including it is antitumor, prevent myocardial infarction, enhance immunity and anti-diabetic Deng wherein especially with the more of the antitumor action research of yew amylose.

The content of the invention

The technical problem to be solved is to provide a kind of regulation and control of yew amylose in raising leaves of Taxus mairei Method, the method is simple and practical, and yew amylose content improves effect substantially, can improve more than 3 times of level.

The present invention solve the technical scheme that adopted of above-mentioned technical problem for：Improve yew amylose in leaves of Taxus mairei Regulation and control method, its regulation and control method is：4～6 days before September part harvests the leaf of 3～5 years raw southerm yew saplings, matter is used Amount percentage concentration is that 0.6～1.2% NaCl solution pours southerm yew sapling, every plant of southerm yew sapling NaCl solution Irrigation amount is 35～40ml；Collect leaves within 4～6 days after pouring, obtain the leaf of yew amylose content raising.

It is 0.9% with mass percentage concentration 5 days before September part harvests the tree China fir leaf of 3 years raw southerm yew saplings NaCl solution pours southerm yew sapling.

Compared with prior art it is an advantage of the invention that improving the regulation and control method of yew amylose in leaves of Taxus mairei, adopt Receive first 4～6 days NaCl solutions with debita spissitudo and pour sapling, 4～6 days collection southerm yew leaves, obtain red after pouring The southerm yew leaf that beans China fir polyoses content is improved, the method is simple and practical, and yew amylose content improves effect substantially, can To improve more than 3 times of level.

Specific embodiment

The present invention is described in further detail with reference to embodiments.

Embodiment 1

5 days before September part harvests the leaf of 3 years raw southerm yew saplings, poured with the NaCl solution of mass percentage concentration 0.9% Sapling is filled, NaCl solution irrigation amount is per plant of 35～40ml, is collected leaves within 5 days after pouring, obtain the south of yew amylose raising Square leaf of Japanese Yew, resonant light scattering（RLS）Method determines yew amylose class content of material in leaf, and 5 days Chinese yew are more after pouring Sugared content reaches highest, and weight percentage is 0.72%, is 4.3 times of blank control group content.

Embodiment 2

Substantially the same manner as Example 1, different simply southerm yew saplings is life in 4 years, and harvesting is 0.6% with concentration in first 6 days NaCl solution pours sapling, 6 days collection southerm yew leaves, resonant light scattering after pouring（RLS）Method determines Chinese yew in leaf Polysaccharose substance content, 6 days yew amylose contents reach highest after pouring, and weight percentage is 0.51%, are that control group contains 3.18 times of amount.

Embodiment 3

Substantially the same manner as Example 1, different simply southerm yew saplings is life in 5 years, and harvesting is 1.2% with concentration in first 4 days NaCl solution pours sapling, 4 days collection southerm yew leaves, resonant light scattering after pouring（RLS）Method determines Chinese yew in leaf Polysaccharose substance content, 4 days yew amylose contents reach highest after pouring, and weight percentage is 0.65%, are that control group contains 4.06 times of amount.

Reference examples

Substantially the same manner as Example 1, the different simply harvesting day before yesterday pour sapling with clear water, 4～6 days collection south after pouring Leaf of Japanese Yew, resonant light scattering（RLS）Method determines yew amylose class content of material in leaf, and 4～6 days Chinese yew are more after pouring Sugared content is 0.16% or so.

Claims

1. the regulation and control method of yew amylose in leaves of Taxus mairei is improved, it is characterised in that regulation and control method is：In the harvesting of September part 4～6 days before the leaf of 3～5 years raw southerm yew saplings, poured with the NaCl solution that mass percentage concentration is 0.6～1.2% Southerm yew sapling, every plant of southerm yew sapling NaCl solution irrigation amount is 35～40ml；4～6 days collection trees after pouring Leaf, obtains the leaf of yew amylose content raising.

2. the regulation and control method of yew amylose in leaves of Taxus mairei is improved as claimed in claim 1, it is characterised in that regulation and control Method is：It is molten with the NaCl that mass percentage concentration is 0.9% 5 days before September part harvests the leaf of 3 years raw southerm yew saplings Liquid pours southerm yew sapling.